Rh5DG533900

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006832
Physical Location & Seq
Forward (+)
82228360 .. 82236273
7914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG533900.1

Sequence Viewer

Length: 4053 bp
ATGGACACGAATTGGAGGCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTACAGCAGGATTCGCGGCAGAGAATCGTCAATAAGATAATGGATACATTGAAGAGGCATCTTCCCTTCTCCGGTCAAGAGGGATTGCATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATACTGCTGCTACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAAATGCTCACTATGGAGACCAAGTCTCAGAACCCAATTGGCACTTCTGTACAAAGCAACTCTGCTGGTAATAGTAACAGGCCCCCTGATCCAGGATCTTTCATGCAACCCCAAGTTCCCAATCAAGGACAGTCACTCCCTATGTCAGTGCCAGCCAATCAATCTCAACCACGCCAACAACTCTTATCACAGAACATTCAGAATAACATTCCGCCCAATTCTGCTGGCTTATCATCTGCATTATCTCCTGCATCTGGGCTTACCCAGACTCCTGTACCGAGTATTGTTGGAAATGCAAACTTGCAAAGTATGTCGGGGACATCACAGAATTCGTTGGGGAACTCCATAGGCCAGGGGGTAACTTCAAATATGTTTGCTAATTCCCAAAGGCAAATGCCAGGACGACAACAGGTAGTTCAGCAGCAGCAACAGCAGCAGCCCCAGAATTCACAGCAGTATATGTACCAGCAACAGTTACAACAACAGCTCATGAAGCCAAAATTTCCGCAGCAGGGAAACATCCAACTTGTGCAAAATCATATGCAACAGCAACAACAGCAGCAGCAGCAGCAGAACTTATTACAACCACCTCAGTTGCAATCTTCTCAGCAATCTGTTATGCAACCTCCTGCAGTGCAGTCGTCTCTCTCAGGTCTTCAGCAGAATCAACAATCTGCTATGCAACACTCTTCACAAGCCATGCTTCAGCAGCATCCTCAATCTGTTCTGCGGCAGCAACAGCAACAGCAACAGCAACAGCAGCCTCAACAGTCTTCTGTTGTCCATCAGCAGCAAACACCAATCCCACAGCAGTCAATATTGCCCCCACAGCAGCAGCAGCAACAGCCACAGCTTATGGTGCAACAGCCAAATTCAACAAACTTGCCGCAGAATCAGCTTATTGGACAACAAAACAATGTAGGGGACATGCAGCAACAGCAACAGCAAAGGTTACTAAGCCAGCAGAATAATTTGTTAAACTTGCAACAGCAGCAGCAGCAGCAGCAACAACAACACCAATTAATGGCTCAGCAAAACAACCTCTCAAATATGCATCAGCAACAGTTGGGCCCTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTTGGTACTCAAACTGGTAACTCAAGCATGCAAACTAATCAGCACTCTGCACACATGCTGCAATCCAAGGTTCAGCAACAACATCAGAGTTCAGCATTACCAATTCAAGGACATCAGTCCCAACCTCAGGCGTCACAGCAACAATTATTATCACAGATTCAATCTCAGCCTGCACAGATACAACAACAGATGGGTATGCAACAGCAATCGAACCCGCTACAACGAGATATGCAGCAAAGGATCCAAGCATCAGGTCAGGTATCAGGATCCATGCTTCAACCACCAAATGTAATGGATCAGCAAAAGCAGTTATATCAAGCTCAAAGAGCCCTTCCTGAGACGTCATCAACGTCTCTAGATTCCACAGCCCAGACTGGACATACAAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGATATGTACTACCCCGAACTAAGTGAAATGTACCAAAAAATTGCTACTAAACTTCATCAGCACGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAATTTCCAAAACTAACATATCACCTGGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATAATAAATTTTATTAATACAAATAGACCAAGGAAGCCTGTTTCTTCTCTGCCGCCAGGGCAGCTTCCCCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAGATTACTCAAGTGCAATCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGACAACAATGCAGCAGAACAATATGACGGGCATGCAGCAAAATTCTATGTCTTCTTTATCCGGGGTTTCAACAGCACAGCAAAACATGATGAATCCATTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAGACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCCTTATCATCACAGAATGGGATTAACATGCTGCAGAGCAACATTAATCCCCTTCAGTCAAATTCTGGTATGCTTCATAGCCAACATCTGAAACAGCAGGAACAGCAATTGTATCAGAATCAACTCAAGCAACAACAATTTCAACAACGACAGATGCAGCAGCAGCAGTTGATGCAGAAGCAGCAGATAATTCAACAACAACAGCAGCAACAGCAACAACAGCTACAACAGCAAGCAAAGCAGCAGCTGCCTGGACAGTTGCAGGCACACCAAATGCCACAGCTTCACCAAATCAGTGATGTTAATGATGTGAAAATAAGGCCGGGGATGGGTGTTAAGCCAGGGGCCTTTCAGCAACATATGCCTGCAGGCCAGCGTGCTTATCCTCATTCGCAATTAAAACCAGGATCTCCATATCCTATTACATCAAGCAACCAACTCCTTCAGGCTCAATCTCCTCAACTGTCACAGCATTCTTCTCCACAAGTTGACCAGCCATTGTTAGTCCCAAAAGCTCAAAATGCTAGTTCACCATTTGTCATCCCATCTCCTTCAACTCCCATAGCTCCATCACCTATGCCAGGTGATTCAGAGAAACCTTCCTCACTCACAAATGCTGGGAATGTTGGACATCAACAAACAACTGCTGTCGGAGCTCAGGTCCAGTCCCTTGCCATCGGCACCCCTGGGATATCAGCCTCTCCTTTGCTTGCTGAATTTATCGGTCCAGATGGCACTCATGCTAATGCTTTGTCAGCTATATCTGGCAAGTCAAGTGTTACAGAGCAACCTCTTGAACGCTTGATTAAAGCGGTCAAGTCAATATCACCAAAGGCATTAAGTGCATCTGTCAGTGACATAGGCTCAGTAGTGAGTATGATTGATAGGATAGCAGGTTCAGCACCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATCTGGTTGCTATGACAAAATGTCGTCTACAGGCGAGAAACTTCATCACTCATGATGGAACAACAAATGGGACAAGGAAAATGAGGCGATACACTAGTGCCATGCCCTTAAATGTTGTGTCATCAGGTGGCAGCATGAATGATAGTTTCAAGCAGTTGACCTGTTCAGAGACATCTGACCTGGAGTCAACTGCAACGTCTCGTATCAAAAGGCCTAGGATTGAGGCTAATCATGCTCTTATGGAAGAAATAAGAGAAATAAATCGGCGGCTTATAGATACAGTTGTTGATATCAGTGATGAAGATGTTGATCCAAGTGCGGCTGCTGCTGAAGGGGGTGAAGGAACCATCGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCCAACTTGAAATCGCAGTATGCTTCAGCACAAATGTCTCCGATTCAGCCATTAAGACTGCTTGTTCCGACAAATTATCCCAACTGCTCTCCAATACTGTTAGACAAGTTTCCGGTTGAAGTCAGTAAGGAGTATGAAGATCTTTCTGTGAAGGCAAAATCAAGGTTTAGCATCTCTTTACGAAGCATTTCGCAGCCCATGTCCCTTGGGGAGATAGCAAGGGCTTGGGATAATTGTGCACGTACAGTTATTTCTGAGCATGCACAGCGAAGTGGTGGAGGCAGCTTCAGCTCAAAGTATGGGACATGGGAGAACTGTCTGAGTGCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1350

Amino Acids

149.05

Weight (kDa)

9.4

Isoelectric Point (pI)

71.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 18 - 95 3e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1700
Acc36I ACCTGC 2 cut(s) 2329, 3284
AccB1I GGYRCC 1 cut(s) 3080
AccB7I CCANNNNNTGG 2 cut(s) 1270, 3311
AccI GTMKAC 1 cut(s) 3364
AccII CGCG 1 cut(s) 85
AcuI CTGAAG 8 cut(s) 887, 935, 1919, 2438, 2828, 3687, 3729, 3991
AcyI GRCGYC 2 cut(s) 1487, 1697
AfaI GTAC 7 cut(s) 297, 522, 710, 1363, 1799, 1823, 3964
AhdI GACNNNNNGTC 2 cut(s) 3357, 3520
AhlI ACTAGT 1 cut(s) 3431
AjuI GAANNNNNNNTTGG 4 cut(s) 274, 306, 1967, 1999
AloI GAACNNNNNNTCC 2 cut(s) 3280, 3312
Alw21I GWGCWC 4 cut(s) 2380, 3058, 3698, 3961
Alw26I GTCTC 8 cut(s) 257, 276, 894, 1688, 1713, 3500, 3540, 3762
Alw44I GTGCAC 1 cut(s) 3957
AlwNI CAGNNNCTG 1 cut(s) 2647
AoxI GGCC 8 cut(s) 17, 326, 595, 1315, 2720, 2745, 2770, 3548
ApaI GGGCCC 1 cut(s) 1319
ApaLI GTGCAC 1 cut(s) 3957
AseI ATTAAT 3 cut(s) 1268, 2019, 2445
Asp700I GAANNNNTTC 1 cut(s) 3907
AspA2I CCTAGG 1 cut(s) 3551
AspLEI GCGC 1 cut(s) 1926
AspS9I GGNCC 7 cut(s) 327, 1315, 1316, 2341, 2745, 3061, 3125
AsuC2I CCSGG 2 cut(s) 2245, 2724
AsuHPI GGTGA 8 cut(s) 1956, 2678, 2922, 2964, 2996, 3219, 3344, 3686
AvaII GGWCC 3 cut(s) 2341, 3061, 3125
AvrII CCTAGG 1 cut(s) 3551
AxyI CCTNAGG 1 cut(s) 1482
BaeGI GKGCMC 2 cut(s) 1319, 3961
BaeI ACNNNNGTAYC 6 cut(s) 504, 537, 3418, 3451, 3606, 3639
BamHI GGATCC 3 cut(s) 1596, 1622, 2302
BanI GGYRCC 1 cut(s) 3080
BanII GRGCYC 4 cut(s) 1319, 1687, 2354, 3058
BarI GAAGNNNNNNTAC 2 cut(s) 1781, 1813
BbsI GAAGAC 3 cut(s) 893, 1011, 2226
Bbv12I GWGCWC 4 cut(s) 2380, 3058, 3698, 3961
BccI CCATC 9 cut(s) 1038, 1540, 2722, 2953, 2977, 3083, 3125, 3386, 3692
BcgI CGANNNNNNTGC 2 cut(s) 867, 901
BciVI GTATCC 1 cut(s) 106
BcnI CCSGG 2 cut(s) 2245, 2724
BcoDI GTCTC 8 cut(s) 257, 276, 894, 1688, 1713, 3500, 3540, 3762
BcuI ACTAGT 1 cut(s) 3431
BfaI CTAG 5 cut(s) 1712, 2925, 3318, 3432, 3552
BfmI CTRYAG 6 cut(s) 876, 2333, 2432, 2766, 3324, 3365
BfoI RGCGCY 1 cut(s) 1927
BfuAI ACCTGC 2 cut(s) 2329, 3284
BfuI GTATCC 1 cut(s) 106
BglI GCCNNNNNGGC 1 cut(s) 2062
BglII AGATCT 1 cut(s) 3859
BlnI CCTAGG 1 cut(s) 3551
BlpI GCTNAGC 1 cut(s) 1275
Bme18I GGWCC 3 cut(s) 2341, 3061, 3125
BmeRI GACNNNNNGTC 2 cut(s) 3357, 3520
BmgT120I GGNCC 7 cut(s) 327, 1315, 1316, 2341, 2745, 3061, 3125
BoxI GACNNNNGTC 2 cut(s) 1471, 2369
BpiI GAAGAC 3 cut(s) 893, 1011, 2226
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 1940, 3539
Bpu10I CCTNAGC 1 cut(s) 3057
Bpu1102I GCTNAGC 1 cut(s) 1275
BpuEI CTTGAG 6 cut(s) 9, 149, 1363, 1910, 2103, 2510
BpuMI CCSGG 2 cut(s) 2245, 2724
BsaAI YACGTR 1 cut(s) 3962
BsaBI GATNNNNATC 1 cut(s) 3645
BsaHI GRCGYC 2 cut(s) 1487, 1697
BsaI GGTCTC 1 cut(s) 257
BsaWI WCCGGW 2 cut(s) 140, 3832
BsaXI ACNNNNNCTCC 8 cut(s) 254, 284, 366, 396, 499, 529, 3045, 3075
Bse1I ACTGG 5 cut(s) 217, 1339, 1375, 1735, 3064
Bse21I CCTNAGG 1 cut(s) 1482
Bse3DI GCAATG 2 cut(s) 48, 2279
Bse8I GATNNNNATC 1 cut(s) 3645
BseGI GGATG 5 cut(s) 52, 765, 958, 2733, 2940
BseJI GATNNNNATC 1 cut(s) 3645
BseMI GCAATG 2 cut(s) 48, 2279
BseNI ACTGG 5 cut(s) 217, 1339, 1375, 1735, 3064
BseRI GAGGAG 4 cut(s) 12, 76, 1775, 2847
BseSI GKGCMC 2 cut(s) 1319, 3961
BseYI CCCAGC 1 cut(s) 3017
BsgI GTGCAG 3 cut(s) 902, 1389, 1512
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 8 cut(s) 19, 328, 597, 1317, 2722, 2747, 2772, 3550
BshNI GGYRCC 1 cut(s) 3080
BsiHKAI GWGCWC 4 cut(s) 2380, 3058, 3698, 3961
BsiSI CCGG 4 cut(s) 141, 2244, 2723, 3833
BsmAI GTCTC 8 cut(s) 257, 276, 894, 1688, 1713, 3500, 3540, 3762
BsmBI CGTCTC 4 cut(s) 894, 1688, 1713, 3540
BsmI GAATGC 2 cut(s) 2404, 2872
BsnI GGCC 8 cut(s) 19, 328, 597, 1317, 2722, 2747, 2772, 3550
Bso31I GGTCTC 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 1315
Bsp1286I GDGCHC 7 cut(s) 1319, 1687, 2354, 2380, 3058, 3698, 3961
Bsp1407I TGTACA 1 cut(s) 295
Bsp1720I GCTNAGC 1 cut(s) 1275
BspANI GGCC 8 cut(s) 19, 328, 597, 1317, 2722, 2747, 2772, 3550
BspFNI CGCG 1 cut(s) 85
BspHI TCATGA 4 cut(s) 735, 1785, 2131, 3388
BspMAI CTGCAG 5 cut(s) 880, 2337, 2436, 2770, 3328
BspMI ACCTGC 2 cut(s) 2329, 3284
BspT107I GGYRCC 1 cut(s) 3080
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 2 cut(s) 48, 2279
BsrGI TGTACA 1 cut(s) 295
BsrI ACTGG 5 cut(s) 217, 1339, 1375, 1735, 3064
BssNI GRCGYC 2 cut(s) 1487, 1697
BssT1I CCWWGG 5 cut(s) 1357, 1422, 2033, 3551, 3925
Bst6I CTCTTC 2 cut(s) 116, 940
BstACI GRCGYC 2 cut(s) 1487, 1697
BstAPI GCANNNNNTGC 6 cut(s) 47, 2092, 2572, 2647, 2761, 3242
BstAUI TGTACA 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 3962
BstENI CCTNNNNNAGG 3 cut(s) 24, 336, 2979
BstF5I GGATG 5 cut(s) 52, 765, 958, 2733, 2940
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 1 cut(s) 1927
BstHHI GCGC 1 cut(s) 1926
BstMAI GTCTC 8 cut(s) 257, 276, 894, 1688, 1713, 3500, 3540, 3762
BstNSI RCATGY 6 cut(s) 1177, 1387, 1414, 2218, 2431, 3983
BstPAI GACNNNNGTC 2 cut(s) 1471, 2369
BstSFI CTRYAG 6 cut(s) 876, 2333, 2432, 2766, 3324, 3365
BstSLI GKGCMC 2 cut(s) 1319, 3961
BstUI CGCG 1 cut(s) 85
BstV2I GAAGAC 3 cut(s) 893, 1011, 2226
BstX2I RGATCY 8 cut(s) 341, 1596, 1622, 2302, 2807, 3314, 3337, 3859
BstYI RGATCY 8 cut(s) 341, 1596, 1622, 2302, 2807, 3314, 3337, 3859
Bsu36I CCTNAGG 1 cut(s) 1482
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 8 cut(s) 19, 328, 597, 1317, 2722, 2747, 2772, 3550
BtsCI GGATG 5 cut(s) 52, 765, 958, 2733, 2940
BtsI GCAGTG 2 cut(s) 885, 2330
BtsIMutI CAGTG 8 cut(s) 399, 885, 2321, 2330, 2380, 2701, 3259, 3637
BveI ACCTGC 2 cut(s) 2329, 3284
CaiI CAGNNNCTG 1 cut(s) 2647
CciI TCATGA 4 cut(s) 735, 1785, 2131, 3388
CfoI GCGC 1 cut(s) 1926
Cfr13I GGNCC 7 cut(s) 327, 1315, 1316, 2341, 2745, 3061, 3125
CseI GACGC 1 cut(s) 1476
CsiI ACCWGGT 2 cut(s) 1966, 3304
Csp6I GTAC 7 cut(s) 296, 521, 709, 1362, 1798, 1822, 3963
CspCI CAANNNNNGTGG 2 cut(s) 822, 857
CviQI GTAC 7 cut(s) 296, 521, 709, 1362, 1798, 1822, 3963
DriI GACNNNNNGTC 2 cut(s) 3357, 3520
Eam1104I CTCTTC 2 cut(s) 116, 940
Eam1105I GACNNNNNGTC 2 cut(s) 3357, 3520
EarI CTCTTC 2 cut(s) 116, 940
EciI GGCGGA 1 cut(s) 447
Ecl136II GAGCTC 1 cut(s) 3056
Eco130I CCWWGG 5 cut(s) 1357, 1422, 2033, 3551, 3925
Eco147I AGGCCT 2 cut(s) 19, 3550
Eco24I GRGCYC 4 cut(s) 1319, 1687, 2354, 3058
Eco31I GGTCTC 1 cut(s) 257
Eco32I GATATC 2 cut(s) 3093, 3628
Eco47I GGWCC 3 cut(s) 2341, 3061, 3125
Eco53kI GAGCTC 1 cut(s) 3056
Eco57I CTGAAG 8 cut(s) 887, 935, 1919, 2438, 2828, 3687, 3729, 3991
Eco81I CCTNAGG 1 cut(s) 1482
EcoICRI GAGCTC 1 cut(s) 3056
EcoNI CCTNNNNNAGG 3 cut(s) 24, 336, 2979
EcoO109I RGGNCCY 4 cut(s) 327, 1316, 2341, 2745
EcoRI GAATTC 2 cut(s) 574, 691
EcoRV GATATC 2 cut(s) 3093, 3628
EcoT14I CCWWGG 5 cut(s) 1357, 1422, 2033, 3551, 3925
EcoT22I ATGCAT 1 cut(s) 1302
EcoT38I GRGCYC 4 cut(s) 1319, 1687, 2354, 3058
ErhI CCWWGG 5 cut(s) 1357, 1422, 2033, 3551, 3925
Esp3I CGTCTC 4 cut(s) 894, 1688, 1713, 3540
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FauI CCCGC 1 cut(s) 1578
FauNDI CATATG 3 cut(s) 786, 2082, 2760
FblI GTMKAC 1 cut(s) 3364
FokI GGATG 5 cut(s) 59, 752, 945, 2740, 2927
FriOI GRGCYC 4 cut(s) 1319, 1687, 2354, 3058
FspBI CTAG 5 cut(s) 1712, 2925, 3318, 3432, 3552
GlaI GCGC 1 cut(s) 1925
GsaI CCCAGC 1 cut(s) 3021
GsuI CTGGAG 2 cut(s) 1940, 3539
HaeII RGCGCY 1 cut(s) 1927
HaeIII GGCC 8 cut(s) 19, 328, 597, 1317, 2722, 2747, 2772, 3550
HapII CCGG 4 cut(s) 141, 2244, 2723, 3833
HgaI GACGC 1 cut(s) 1476
HhaI GCGC 1 cut(s) 1926
Hin1I GRCGYC 2 cut(s) 1487, 1697
Hin6I GCGC 1 cut(s) 1924
HinP1I GCGC 1 cut(s) 1924
HincII GTYRAC 3 cut(s) 2890, 3495, 3525
HindII GTYRAC 3 cut(s) 2890, 3495, 3525
HpaII CCGG 4 cut(s) 141, 2244, 2723, 3833
HphI GGTGA 8 cut(s) 1956, 2678, 2922, 2964, 2996, 3219, 3344, 3686
Hpy166II GTNNAC 6 cut(s) 2890, 2930, 3365, 3495, 3525, 3959
Hpy8I GTNNAC 6 cut(s) 2890, 2930, 3365, 3495, 3525, 3959
HpyCH4IV ACGT 4 cut(s) 1697, 1706, 3533, 3961
HpySE526I ACGT 4 cut(s) 1697, 1706, 3533, 3961
Hsp92I GRCGYC 2 cut(s) 1487, 1697
HspAI GCGC 1 cut(s) 1924
LmnI GCTCC 5 cut(s) 63, 1921, 2383, 2971, 3053
MabI ACCWGGT 2 cut(s) 1966, 3304
MaeI CTAG 5 cut(s) 1712, 2925, 3318, 3432, 3552
MaeII ACGT 4 cut(s) 1697, 1706, 3533, 3961
MfeI CAATTG 3 cut(s) 282, 2153, 2507
MflI RGATCY 8 cut(s) 341, 1596, 1622, 2302, 2807, 3314, 3337, 3859
MhlI GDGCHC 7 cut(s) 1319, 1687, 2354, 2380, 3058, 3698, 3961
MlyI GAGTC 4 cut(s) 508, 1850, 2359, 3530
MmeI TCCRAC 7 cut(s) 201, 514, 793, 2383, 3007, 3031, 3812
Mph1103I ATGCAT 1 cut(s) 1302
MroXI GAANNNNTTC 1 cut(s) 3907
MslI CAYNNNNRTG 2 cut(s) 1740, 3144
MspA1I CMGCKG 1 cut(s) 2647
MspI CCGG 4 cut(s) 141, 2244, 2723, 3833
MunI CAATTG 3 cut(s) 282, 2153, 2507
Mva1269I GAATGC 2 cut(s) 2404, 2872
MvnI CGCG 1 cut(s) 85
NciI CCSGG 2 cut(s) 2245, 2724
NdeI CATATG 3 cut(s) 786, 2082, 2760
NmuCI GTSAC 6 cut(s) 378, 1488, 2100, 2182, 2865, 3254
NsiI ATGCAT 1 cut(s) 1302
NspI RCATGY 6 cut(s) 1177, 1387, 1414, 2218, 2431, 3983
PaeI GCATGC 3 cut(s) 1387, 2218, 3983
PagI TCATGA 4 cut(s) 735, 1785, 2131, 3388
PasI CCCWGGG 1 cut(s) 3086
PceI AGGCCT 2 cut(s) 19, 3550
PcsI WCGNNNNNNNCGW 1 cut(s) 1703
PctI GAATGC 2 cut(s) 2404, 2872
PdmI GAANNNNTTC 1 cut(s) 3907
PflFI GACNNNGTC 1 cut(s) 268
PflMI CCANNNNNTGG 2 cut(s) 1270, 3311
PfoI TCCNGGA 2 cut(s) 337, 2305
PleI GAGTC 4 cut(s) 508, 1850, 2359, 3529
PpsI GAGTC 4 cut(s) 508, 1850, 2359, 3529
Ppu21I YACGTR 1 cut(s) 3962
PpuMI RGGWCCY 1 cut(s) 2341
PshAI GACNNNNGTC 2 cut(s) 1471, 2369
PshBI ATTAAT 3 cut(s) 1268, 2019, 2445
Psp124BI GAGCTC 1 cut(s) 3058
Psp5II RGGWCCY 1 cut(s) 2341
PspFI CCCAGC 1 cut(s) 3017
PspOMI GGGCCC 1 cut(s) 1315
PspPI GGNCC 7 cut(s) 327, 1315, 1316, 2341, 2745, 3061, 3125
PspPPI RGGWCCY 1 cut(s) 2341
PstI CTGCAG 5 cut(s) 880, 2337, 2436, 2770, 3328
PstNI CAGNNNCTG 1 cut(s) 2647
PsuI RGATCY 8 cut(s) 341, 1596, 1622, 2302, 2807, 3314, 3337, 3859
PsyI GACNNNGTC 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 2647
RsaI GTAC 7 cut(s) 297, 522, 710, 1363, 1799, 1823, 3964
RsaNI GTAC 7 cut(s) 296, 521, 709, 1362, 1798, 1822, 3963
RseI CAYNNNNRTG 2 cut(s) 1740, 3144
SacI GAGCTC 1 cut(s) 3058
Sau96I GGNCC 7 cut(s) 327, 1315, 1316, 2341, 2745, 3061, 3125
SbfI CCTGCAGG 1 cut(s) 2770
SchI GAGTC 4 cut(s) 508, 1850, 2359, 3530
SdaI CCTGCAGG 1 cut(s) 2770
SduI GDGCHC 7 cut(s) 1319, 1687, 2354, 2380, 3058, 3698, 3961
SexAI ACCWGGT 2 cut(s) 1966, 3304
SfcI CTRYAG 6 cut(s) 876, 2333, 2432, 2766, 3324, 3365
SinI GGWCC 3 cut(s) 2341, 3061, 3125
SmiMI CAYNNNNRTG 2 cut(s) 1740, 3144
SmlI CTYRAG 6 cut(s) 24, 164, 1378, 1889, 2118, 2525
SmoI CTYRAG 6 cut(s) 24, 164, 1378, 1889, 2118, 2525
SpeI ACTAGT 1 cut(s) 3431
SphI GCATGC 3 cut(s) 1387, 2218, 3983
Sse8387I CCTGCAGG 1 cut(s) 2770
SseBI AGGCCT 2 cut(s) 19, 3550
SspI AATATT 1 cut(s) 1065
SspMI CTAG 5 cut(s) 1712, 2925, 3318, 3432, 3552
SstI GAGCTC 1 cut(s) 3058
StuI AGGCCT 2 cut(s) 19, 3550
StyI CCWWGG 5 cut(s) 1357, 1422, 2033, 3551, 3925
TaiI ACGT 4 cut(s) 1700, 1709, 3536, 3964
TaqI TCGA 1 cut(s) 1565
TaqII GACCGA 1 cut(s) 3113
TatI WGTACW 2 cut(s) 295, 1797
TauI GCSGC 7 cut(s) 88, 979, 1135, 2059, 3607, 3659, 4049
TscAI CASTG 8 cut(s) 399, 885, 2328, 2337, 2380, 2701, 3259, 3637
TseFI GTSAC 6 cut(s) 378, 1488, 2100, 2182, 2865, 3254
Tsp45I GTSAC 6 cut(s) 378, 1488, 2100, 2182, 2865, 3254
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 8 cut(s) 399, 885, 2328, 2337, 2380, 2701, 3259, 3637
Tth111I GACNNNGTC 1 cut(s) 268
Van91I CCANNNNNTGG 2 cut(s) 1270, 3311
VneI GTGCAC 1 cut(s) 3957
VpaK11BI GGWCC 3 cut(s) 2341, 3061, 3125
VspI ATTAAT 3 cut(s) 1268, 2019, 2445
XagI CCTNNNNNAGG 3 cut(s) 24, 336, 2979
XbaI TCTAGA 2 cut(s) 1711, 3317
XceI RCATGY 6 cut(s) 1177, 1387, 1414, 2218, 2431, 3983
XmaJI CCTAGG 1 cut(s) 3551
XmiI GTMKAC 1 cut(s) 3364
XmnI GAANNNNTTC 1 cut(s) 3907
XspI CTAG 5 cut(s) 1712, 2925, 3318, 3432, 3552
ZraI GACGTC 1 cut(s) 1698
Zsp2I ATGCAT 1 cut(s) 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.