FvH4_3g44090

X-Pro dipeptidyl-peptidase (S15 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009940
Physical Location & Seq
Reverse (-)
36781244 .. 36784543
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44090.t1

Sequence Viewer

Length: 672 bp
ATGTCAAACTGTACTGTTGAGACCTGCACAGTTGAGACCAGTGATGGAGCCAAGCTCAACACACGGCTCTTCAGACCCAGAGAAGCCAAAGAGGGCAATGATCCTGTGGTGGTTCTGGTCCATCCCTACTCTGTCCTTGGTGGCTGTCAAGGCCTTTTGAGAGGCATGGCAACTGGGTTAGCTGAGAAAGGTTTGCAAGCTGTCACCTTTGACATGAGAGGAGTTGGGAGGTCAACTGGGAAGGCTTCTCTTACTGGGTTTGCTGAAATCAAAGATGTCATTGCTGTCTGCAAATGGGTCTCTGACAATTTGGATGCCAATAGAATTTTGCTTGTGGGTTCTTCTGCAGGTGCCCCAATTGCAGGGTCAGCAGTTGATCAGATTGAACAAGTTGTGGGATATGTGAGTCTGGGGTATCCTTTTGGCATGACTGCCTCAATTCTTTTTGGGAGACACCACAAAGCCATCCTACAATCCCCGAAACCAAAACTTTTTGTAATGGGGACTCGAGATGGGTTTACAAGTGTGAAGCAACTGAAGAACAAGCTAAGTTCTGCGGCAGGGCGTGTCGAAACACACCTTATTGAAGGAGTTAGCCACTTCCAAATGGAAGGCGCAGCTTATGATACACAGATGGTGAACCTTATCCTTGAATTTATTGTATCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S15

Protein Analysis

224

Amino Acids

23.77

Weight (kDa)

8.34

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S15 PF02129 15 - 116 2e-11 X-Pro dipeptidyl-peptidase (S15 family)
Abhydrolase_1 PF00561 49 - 128 1.7e-06 alpha/beta hydrolase fold
Hydrolase_4 PF12146 51 - 138 1.4e-06 Serine aminopeptidase, S33
Abhydrolase_11 PF20408 53 - 200 4.7e-10 Alpha/beta hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 338
Acc36I ACCTGC 2 cut(s) 32, 338
AccB1I GGYRCC 1 cut(s) 350
AciI CCGC 1 cut(s) 557
AclWI GGATC 1 cut(s) 95
AcsI RAATTY 2 cut(s) 324, 653
AcuI CTGAAG 2 cut(s) 55, 557
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 3 cut(s) 386, 587, 653
AluBI AGCT 5 cut(s) 55, 182, 200, 547, 620
AluI AGCT 5 cut(s) 55, 182, 200, 547, 620
Alw26I GTCTC 4 cut(s) 14, 29, 304, 445
AlwI GGATC 1 cut(s) 95
Ama87I CYCGRG 1 cut(s) 507
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 617
ApoI RAATTY 2 cut(s) 324, 653
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 196, 649
AvaI CYCGRG 1 cut(s) 507
AvaII GGWCC 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 350
BbvI GCAGC 1 cut(s) 629
BccI CCATC 5 cut(s) 38, 129, 473, 506, 628
BceAI ACGGC 1 cut(s) 80
BciVI GTATCC 1 cut(s) 426
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 4 cut(s) 14, 29, 304, 445
BfmI CTRYAG 1 cut(s) 345
BfuAI ACCTGC 2 cut(s) 32, 338
BfuI GTATCC 1 cut(s) 426
BisI GCNGC 2 cut(s) 558, 618
BlsI GCNGC 2 cut(s) 559, 619
Bme18I GGWCC 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 507
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 2 cut(s) 49, 352
BmrI ACTGGG 3 cut(s) 183, 246, 264
BmsI GCATC 1 cut(s) 304
BmuI ACTGGG 3 cut(s) 183, 246, 264
BplI GAGNNNNNCTC 2 cut(s) 39, 71
BsaI GGTCTC 3 cut(s) 14, 29, 304
BsaJI CCNNGG 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse1I ACTGG 4 cut(s) 39, 178, 241, 259
Bse3DI GCAATG 2 cut(s) 103, 279
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 121, 319, 465
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMI GCAATG 2 cut(s) 103, 279
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 4 cut(s) 39, 178, 241, 259
BseRI GAGGAG 1 cut(s) 234
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 1 cut(s) 629
BsgI GTGCAG 1 cut(s) 10
BshFI GGCC 1 cut(s) 153
BshNI GGYRCC 1 cut(s) 350
BsiHKCI CYCGRG 1 cut(s) 507
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 4 cut(s) 14, 29, 304, 445
BsmFI GGGAC 1 cut(s) 517
BsnI GGCC 1 cut(s) 153
Bso31I GGTCTC 3 cut(s) 14, 29, 304
BsoBI CYCGRG 1 cut(s) 507
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 2 cut(s) 100, 376
BspACI CCGC 1 cut(s) 557
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 49, 352
BspMAI CTGCAG 1 cut(s) 349
BspMI ACCTGC 2 cut(s) 32, 338
BspPI GGATC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 3 cut(s) 14, 29, 304
BsrDI GCAATG 2 cut(s) 103, 279
BsrI ACTGG 4 cut(s) 39, 178, 241, 259
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 100, 376
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 3 cut(s) 11, 16, 31
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 2 cut(s) 183, 548
BstF5I GGATG 3 cut(s) 121, 319, 465
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 2 cut(s) 103, 379
BstMAI GTCTC 4 cut(s) 14, 29, 304, 445
BstMBI GATC 2 cut(s) 100, 376
BstMWI GCNNNNNNNGC 3 cut(s) 150, 359, 368
BstSFI CTRYAG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 629
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 1 cut(s) 153
BtsCI GGATG 3 cut(s) 121, 319, 465
BtsIMutI CAGTG 1 cut(s) 46
BveI ACCTGC 2 cut(s) 32, 338
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 12
CviAII CATG 3 cut(s) 166, 214, 427
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 2 cut(s) 183, 548
DpnI GATC 2 cut(s) 102, 378
DpnII GATC 2 cut(s) 100, 376
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 153
Eco31I GGTCTC 3 cut(s) 14, 29, 304
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 2 cut(s) 55, 557
Eco88I CYCGRG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 3 cut(s) 169, 217, 430
FaiI YATR 5 cut(s) 167, 215, 402, 428, 624
FaqI GGGAC 1 cut(s) 517
FatI CATG 3 cut(s) 165, 213, 426
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 2 cut(s) 558, 618
FokI GGATG 3 cut(s) 108, 326, 452
Fsp4HI GCNGC 2 cut(s) 558, 618
GlaI GCGC 1 cut(s) 616
GluI GCNGC 2 cut(s) 558, 618
HaeIII GGCC 1 cut(s) 153
HhaI GCGC 1 cut(s) 617
Hin1II CATG 3 cut(s) 169, 217, 430
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HinfI GANTC 2 cut(s) 406, 505
HphI GGTGA 2 cut(s) 196, 649
Hpy166II GTNNAC 3 cut(s) 234, 519, 640
Hpy188I TCNGA 3 cut(s) 74, 304, 381
Hpy188III TCNNGA 1 cut(s) 509
Hpy8I GTNNAC 3 cut(s) 234, 519, 640
HpyAV CCTTC 3 cut(s) 235, 581, 605
HpyCH4III ACNGT 3 cut(s) 11, 16, 31
HpyCH4V TGCA 5 cut(s) 27, 196, 291, 347, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 359, 368
HpyF3I CTNAG 2 cut(s) 183, 548
Hsp92II CATG 3 cut(s) 169, 217, 430
HspAI GCGC 1 cut(s) 615
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 2 cut(s) 100, 376
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 1 cut(s) 629
LweI GCATC 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 202
MalI GATC 2 cut(s) 102, 378
MboI GATC 2 cut(s) 100, 376
MboII GAAGA 3 cut(s) 61, 333, 550
MfeI CAATTG 1 cut(s) 357
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 5 cut(s) 307, 324, 357, 438, 653
MlyI GAGTC 2 cut(s) 415, 499
MnlI CCTC 5 cut(s) 85, 155, 212, 222, 445
MunI CAATTG 1 cut(s) 357
MwoI GCNNNNNNNGC 3 cut(s) 150, 359, 368
NdeII GATC 2 cut(s) 100, 376
NlaIII CATG 3 cut(s) 169, 217, 430
NlaIV GGNNCC 2 cut(s) 49, 352
NmuCI GTSAC 1 cut(s) 202
PaeR7I CTCGAG 1 cut(s) 507
PaqCI CACCTGC 1 cut(s) 338
PceI AGGCCT 1 cut(s) 153
PciSI GCTCTTC 1 cut(s) 74
PkrI GCNGC 2 cut(s) 559, 619
PleI GAGTC 2 cut(s) 414, 499
PpsI GAGTC 2 cut(s) 414, 499
PspN4I GGNNCC 2 cut(s) 49, 352
PspPI GGNCC 1 cut(s) 118
PstI CTGCAG 1 cut(s) 349
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 74
SatI GCNGC 2 cut(s) 558, 618
Sau3AI GATC 2 cut(s) 100, 376
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 2 cut(s) 415, 499
SduI GDGCHC 1 cut(s) 355
SfaNI GCATC 1 cut(s) 304
SfcI CTRYAG 1 cut(s) 345
Sfr274I CTCGAG 1 cut(s) 507
SinI GGWCC 1 cut(s) 118
SlaI CTCGAG 1 cut(s) 507
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
Sse9I AATT 5 cut(s) 307, 324, 357, 438, 653
SseBI AGGCCT 1 cut(s) 153
SsiI CCGC 1 cut(s) 557
StuI AGGCCT 1 cut(s) 153
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 11, 16, 31
TaqI TCGA 2 cut(s) 508, 570
TasI AATT 5 cut(s) 307, 324, 357, 438, 653
TatI WGTACW 1 cut(s) 11
TauI GCSGC 1 cut(s) 560
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 617
Tsp45I GTSAC 1 cut(s) 202
TspRI CASTG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 2 cut(s) 324, 653
XhoI CTCGAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.