Rh5BG544100

X-Pro dipeptidyl-peptidase (S15 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009940
Physical Location & Seq
Reverse (-)
86649460 .. 86651844
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG544100.1

Sequence Viewer

Length: 690 bp
ATGTCAAACTGTACGGTCGAGACCTGCACAGTTGAGACCAGTGATGGAGCCAAGCTCAACACAAGGCTCTTCAGACCCAGAGAAGCCCAACAACAACATCAAATGACGGAGGGCAATGATCTTGTGGTGGTTCTGGTGCATCCCTACTCTGTCCTGGGTGGCTGTCAGGGACTTTTGAGAGGCATAGCAGCTGGGTTAGCTGAGAAAGGCTTCAAAGCTGTCACTTTTGACATGAGAGGAGTGGGGAGGTCAACTGGGAAGGCTTCTCTTACCGGGTTTGCAGAAATCAAGGATGTGATTGCTGTCTGCAAATGGGTCTCTGAGAATTTGTCTGCTGATAGAATTTTGCTTGTGGGTTCTTCTGCAGGTGCCCCGATTGCAGGCTCAGCGGTTGATCAGATTGAACAAGTTGTAGGATATGTGAGTCTGGGGTATCCTTTTGGCATGACTGCCTCGATCCTTTTTGGGAGACACCACAAAGCCATCCTACAATCCCCGAAACCAAAACTTTTTGTAATGGGGACTCGAGATGGGTTTACAAGTGTGAAGCAACTGAAGAACAAGCTAAGTTCTGCAGCAGGGCGTGTTGAAACACACCTTATTGAAGGAGTTAGCCACTTCCAAATGGAAGGCGCAGCTTATGATACACAGATGGTGAATCTTATCCTTGAATTTATTGTGTCGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S15

Protein Analysis

229

Amino Acids

24.52

Weight (kDa)

8.34

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S15 PF02129 15 - 124 6.5e-09 X-Pro dipeptidyl-peptidase (S15 family)
Hydrolase_4 PF12146 57 - 151 5.6e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 59 - 134 5.8e-08 alpha/beta hydrolase fold
Abhydrolase_11 PF20408 61 - 206 1.2e-09 Alpha/beta hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 356
Acc36I ACCTGC 2 cut(s) 32, 356
AccB1I GGYRCC 1 cut(s) 368
AciI CCGC 1 cut(s) 389
AclWI GGATC 1 cut(s) 451
AcsI RAATTY 3 cut(s) 325, 342, 671
AcuI CTGAAG 2 cut(s) 55, 575
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 380
AgsI TTSAA 5 cut(s) 214, 404, 590, 605, 671
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 6 cut(s) 55, 191, 200, 218, 565, 638
AluI AGCT 6 cut(s) 55, 191, 200, 218, 565, 638
Alw26I GTCTC 4 cut(s) 14, 29, 322, 463
AlwI GGATC 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 525
ApeKI GCWGC 3 cut(s) 188, 575, 635
ApoI RAATTY 3 cut(s) 325, 342, 671
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 1 cut(s) 635
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 1 cut(s) 667
AvaI CYCGRG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 368
BbvI GCAGC 3 cut(s) 200, 587, 647
BccI CCATC 4 cut(s) 38, 491, 524, 646
BciT130I CCWGG 1 cut(s) 155
BciVI GTATCC 1 cut(s) 444
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 4 cut(s) 14, 29, 322, 463
BfmI CTRYAG 2 cut(s) 363, 573
BfuAI ACCTGC 2 cut(s) 32, 356
BfuI GTATCC 1 cut(s) 444
BisI GCNGC 3 cut(s) 189, 576, 636
BlpI GCTNAGC 1 cut(s) 385
BlsI GCNGC 3 cut(s) 190, 577, 637
Bme1390I CCNGG 2 cut(s) 155, 274
BmeT110I CYCGRG 1 cut(s) 525
BmiI GGNNCC 2 cut(s) 49, 370
BmrFI CCNGG 2 cut(s) 155, 274
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 264
BplI GAGNNNNNCTC 2 cut(s) 39, 71
Bpu1102I GCTNAGC 1 cut(s) 385
BpuMI CCSGG 1 cut(s) 274
BsaI GGTCTC 3 cut(s) 14, 29, 322
BsaJI CCNNGG 1 cut(s) 154
Bsc4I CCNNNNNNNGG 1 cut(s) 380
Bse1I ACTGG 2 cut(s) 39, 259
Bse3DI GCAATG 1 cut(s) 121
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 3 cut(s) 139, 298, 483
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 3 cut(s) 192, 312, 399
BseNI ACTGG 2 cut(s) 39, 259
BseRI GAGGAG 1 cut(s) 252
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 3 cut(s) 200, 587, 647
BseYI CCCAGC 1 cut(s) 191
BsgI GTGCAG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 18
BshNI GGYRCC 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 525
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 2 cut(s) 183, 535
BslI CCNNNNNNNGG 1 cut(s) 380
BsmAI GTCTC 4 cut(s) 14, 29, 322, 463
BsmFI GGGAC 2 cut(s) 183, 535
Bso31I GGTCTC 3 cut(s) 14, 29, 322
BsoBI CYCGRG 1 cut(s) 525
Bsp1286I GDGCHC 1 cut(s) 373
Bsp143I GATC 3 cut(s) 118, 394, 456
Bsp1720I GCTNAGC 1 cut(s) 385
BspACI CCGC 1 cut(s) 389
BspCNI CTCAG 3 cut(s) 193, 313, 398
BspLI GGNNCC 2 cut(s) 49, 370
BspMAI CTGCAG 2 cut(s) 367, 577
BspMI ACCTGC 2 cut(s) 32, 356
BspPI GGATC 1 cut(s) 451
BspQI GCTCTTC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 368
BspTNI GGTCTC 3 cut(s) 14, 29, 322
BsrDI GCAATG 1 cut(s) 121
BsrI ACTGG 2 cut(s) 39, 259
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 118, 394, 456
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 3 cut(s) 11, 16, 31
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 4 cut(s) 201, 321, 385, 566
BstF5I GGATG 3 cut(s) 139, 298, 483
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 3 cut(s) 121, 397, 459
BstMAI GTCTC 4 cut(s) 14, 29, 322, 463
BstMBI GATC 3 cut(s) 118, 394, 456
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 3 cut(s) 197, 377, 386
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 153, 272
BstSFI CTRYAG 2 cut(s) 363, 573
BstSLI GKGCMC 1 cut(s) 373
BstV1I GCAGC 3 cut(s) 200, 587, 647
BsuI GTATCC 1 cut(s) 444
BtsCI GGATG 3 cut(s) 139, 298, 483
BtsIMutI CAGTG 1 cut(s) 46
BveI ACCTGC 2 cut(s) 32, 356
Cac8I GCNNGC 1 cut(s) 382
CfoI GCGC 1 cut(s) 635
Csp6I GTAC 1 cut(s) 12
CviAII CATG 2 cut(s) 232, 445
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 4 cut(s) 201, 321, 385, 566
DpnI GATC 3 cut(s) 120, 396, 458
DpnII GATC 3 cut(s) 118, 394, 456
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco31I GGTCTC 3 cut(s) 14, 29, 322
Eco57I CTGAAG 2 cut(s) 55, 575
Eco88I CYCGRG 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 153
FaeI CATG 2 cut(s) 235, 448
FaiI YATR 5 cut(s) 185, 233, 420, 446, 642
FaqI GGGAC 2 cut(s) 183, 535
FatI CATG 2 cut(s) 231, 444
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 3 cut(s) 189, 576, 636
FokI GGATG 3 cut(s) 126, 305, 470
Fsp4HI GCNGC 3 cut(s) 189, 576, 636
GlaI GCGC 1 cut(s) 634
GluI GCNGC 3 cut(s) 189, 576, 636
GsaI CCCAGC 1 cut(s) 195
HapII CCGG 1 cut(s) 273
HhaI GCGC 1 cut(s) 635
Hin1II CATG 2 cut(s) 235, 448
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 3 cut(s) 424, 523, 658
HpaII CCGG 1 cut(s) 273
HphI GGTGA 1 cut(s) 667
Hpy166II GTNNAC 2 cut(s) 252, 537
Hpy188I TCNGA 3 cut(s) 74, 322, 399
Hpy188III TCNNGA 2 cut(s) 19, 527
Hpy8I GTNNAC 2 cut(s) 252, 537
HpyAV CCTTC 3 cut(s) 253, 599, 623
HpyCH4III ACNGT 3 cut(s) 11, 16, 31
HpyCH4V TGCA 7 cut(s) 27, 139, 281, 309, 365, 380, 575
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 377, 386
HpyF3I CTNAG 4 cut(s) 201, 321, 385, 566
Hsp92II CATG 2 cut(s) 235, 448
HspAI GCGC 1 cut(s) 633
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 3 cut(s) 118, 394, 456
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 3 cut(s) 200, 587, 647
LweI GCATC 1 cut(s) 148
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 120, 396, 458
MboI GATC 3 cut(s) 118, 394, 456
MboII GAAGA 3 cut(s) 61, 351, 568
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 3 cut(s) 325, 342, 671
MlyI GAGTC 2 cut(s) 433, 517
MnlI CCTC 5 cut(s) 103, 173, 230, 240, 463
MroXI GAANNNNTTC 1 cut(s) 209
MspA1I CMGCKG 2 cut(s) 191, 389
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 2 cut(s) 155, 274
MvaI CCWGG 1 cut(s) 155
MwoI GCNNNNNNNGC 3 cut(s) 197, 377, 386
NciI CCSGG 1 cut(s) 274
NdeII GATC 3 cut(s) 118, 394, 456
NlaIII CATG 2 cut(s) 235, 448
NlaIV GGNNCC 2 cut(s) 49, 370
NmuCI GTSAC 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 525
PaqCI CACCTGC 1 cut(s) 356
PciSI GCTCTTC 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 658
PkrI GCNGC 3 cut(s) 190, 577, 637
PleI GAGTC 2 cut(s) 432, 517
PpsI GAGTC 2 cut(s) 432, 517
Psp6I CCWGG 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 49, 370
PstI CTGCAG 2 cut(s) 367, 577
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 74
SatI GCNGC 3 cut(s) 189, 576, 636
Sau3AI GATC 3 cut(s) 118, 394, 456
SchI GAGTC 2 cut(s) 433, 517
ScrFI CCNGG 2 cut(s) 155, 274
SduI GDGCHC 1 cut(s) 373
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 2 cut(s) 363, 573
Sfr274I CTCGAG 1 cut(s) 525
SlaI CTCGAG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 525
SmoI CTYRAG 1 cut(s) 525
Sse9I AATT 3 cut(s) 325, 342, 671
SsiI CCGC 1 cut(s) 389
StyD4I CCNGG 2 cut(s) 153, 272
TaaI ACNGT 3 cut(s) 11, 16, 31
TaqI TCGA 3 cut(s) 18, 455, 526
TasI AATT 3 cut(s) 325, 342, 671
TfiI GAWTC 1 cut(s) 658
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 3 cut(s) 188, 575, 635
Tsp45I GTSAC 1 cut(s) 220
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 1 cut(s) 46
XapI RAATTY 3 cut(s) 325, 342, 671
XhoI CTCGAG 1 cut(s) 525
XmnI GAANNNNTTC 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.