MD03G1018500.v1.1

X-Pro dipeptidyl-peptidase (S15 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009940
Physical Location & Seq
Forward (+)
1441878 .. 1443680
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1018500.v1.1.491

Sequence Viewer

Length: 678 bp
ATGTCAAACTGTACGGTGGAGCGCTGCACAGTTGAGACCAGCGATGGAGTCAAGCTCAGCGCAAAGCTCTTCAAACCCAGAGAAGAAGAAGAGATCAAGAAGGGCAGCCCTGTGGTGGTTCTGGTGCACCCATACTCCGTCCTCGGCGGCTGCCAAGGCCTGTTGAGAGGAATAGCAGCTGGGTTGGCAGACGGAGGCTATAAAGCTTTGACCTTTGACATGAGAGGGGTTGGGAGGTCAACAGGAAGGGCCTCTCTCACTGGGTTTGCAGAAATTAAGGATGTGATTGCTGTGTGCAAATGGGTTTCTGAGAATCAATCAGCTGACAGGATTTTGTTGGTGGGTTCTTCTGCAGGTGCCCCGATTGCAGGCTCTGCGGTAGATCAGGTTGAACAAGTTGTGGGCTATGTTAGTCTGGGGTATCCTTTCGGCATGCTTGCCTCAATCCTTTTCGGGAGACACCACAAAGCCGTACTACAATCCTCAAAACCCAAACTTTTCGTAATGGGGACACAGGACGGGTTCACGAGCGTGAAGCAGTTAAAGAACAAGCTGAGTTCTGCAGCCGGTCGTGTTGAAACACACCTGATTGAAGGAGCAGGCCACTTCCAAATGGAAGGCCCTGCTTATGATGCACAGATGGTGAATCTTATCCTTGAATTCATTGCATCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S15

Protein Analysis

226

Amino Acids

23.85

Weight (kDa)

8.32

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S15 PF02129 15 - 122 2.7e-11 X-Pro dipeptidyl-peptidase (S15 family)
Abhydrolase_1 PF00561 51 - 130 6.6e-06 alpha/beta hydrolase fold
Hydrolase_4 PF12146 53 - 137 8.5e-08 Serine aminopeptidase, S33
Abhydrolase_11 PF20408 96 - 202 3.7e-09 Alpha/beta hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 356
AciI CCGC 2 cut(s) 147, 377
AcsI RAATTY 1 cut(s) 659
AfaI GTAC 2 cut(s) 13, 474
AfeI AGCGCT 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 115, 368
AgsI TTSAA 5 cut(s) 73, 392, 578, 593, 659
AleI CACNNNNGTG 1 cut(s) 530
AluBI AGCT 6 cut(s) 55, 67, 179, 206, 323, 553
AluI AGCT 6 cut(s) 55, 67, 179, 206, 323, 553
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 29, 451
Alw44I GTGCAC 1 cut(s) 125
AlwNI CAGNNNCTG 1 cut(s) 374
Aor51HI AGCGCT 1 cut(s) 23
AoxI GGCC 4 cut(s) 157, 249, 601, 619
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 5 cut(s) 24, 105, 150, 176, 563
ApoI RAATTY 1 cut(s) 659
AspLEI GCGC 2 cut(s) 24, 62
AspS9I GGNCC 2 cut(s) 249, 620
AsuHPI GGTGA 1 cut(s) 655
BaeGI GKGCMC 2 cut(s) 129, 361
BanI GGYRCC 1 cut(s) 356
BauI CACGAG 1 cut(s) 526
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 5 cut(s) 11, 117, 137, 188, 575
BccI CCATC 2 cut(s) 38, 634
BceAI ACGGC 1 cut(s) 455
BciVI GTATCC 1 cut(s) 432
BcoDI GTCTC 2 cut(s) 29, 451
BfmI CTRYAG 2 cut(s) 351, 561
BfoI RGCGCY 1 cut(s) 25
BfuAI ACCTGC 1 cut(s) 344
BfuI GTATCC 1 cut(s) 432
BisI GCNGC 6 cut(s) 25, 106, 148, 151, 177, 564
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 6 cut(s) 26, 107, 149, 152, 178, 565
BmgT120I GGNCC 2 cut(s) 249, 620
BmiI GGNNCC 1 cut(s) 358
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 622
BmuI ACTGGG 1 cut(s) 270
BplI GAGNNNNNCTC 2 cut(s) 39, 71
Bpu1102I GCTNAGC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 142, 154
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 368
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 663
BseDI CCNNGG 2 cut(s) 142, 154
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 115, 368
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 3 cut(s) 70, 300, 545
BseNI ACTGG 1 cut(s) 265
BseSI GKGCMC 2 cut(s) 129, 361
BseXI GCAGC 5 cut(s) 11, 117, 137, 188, 575
BseYI CCCAGC 1 cut(s) 179
BsgI GTGCAG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 571
BshFI GGCC 4 cut(s) 159, 251, 603, 621
BshNI GGYRCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 1 cut(s) 567
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 2 cut(s) 115, 368
BsmAI GTCTC 2 cut(s) 29, 451
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 4 cut(s) 159, 251, 603, 621
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 129, 361
Bsp143I GATC 2 cut(s) 93, 382
Bsp1720I GCTNAGC 1 cut(s) 56
BspACI CCGC 2 cut(s) 147, 377
BspANI GGCC 4 cut(s) 159, 251, 603, 621
BspCNI CTCAG 3 cut(s) 69, 301, 546
BspLI GGNNCC 1 cut(s) 358
BspMAI CTGCAG 2 cut(s) 355, 565
BspMI ACCTGC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 663
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 2 cut(s) 142, 154
BssMI GATC 2 cut(s) 93, 382
BssSI CACGAG 1 cut(s) 526
BssT1I CCWWGG 1 cut(s) 154
Bst2BI CACGAG 1 cut(s) 526
Bst4CI ACNGT 3 cut(s) 11, 16, 31
Bst6I CTCTTC 2 cut(s) 74, 84
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 4 cut(s) 370, 434, 438, 601
BstDEI CTNAG 3 cut(s) 56, 309, 554
BstF5I GGATG 1 cut(s) 286
BstH2I RGCGCY 1 cut(s) 25
BstHHI GCGC 2 cut(s) 24, 62
BstKTI GATC 2 cut(s) 96, 385
BstMAI GTCTC 2 cut(s) 29, 451
BstMBI GATC 2 cut(s) 93, 382
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 5 cut(s) 156, 185, 365, 374, 632
BstNSI RCATGY 1 cut(s) 436
BstSFI CTRYAG 2 cut(s) 351, 561
BstSLI GKGCMC 2 cut(s) 129, 361
BstV1I GCAGC 5 cut(s) 11, 117, 137, 188, 575
BsuI GTATCC 1 cut(s) 432
BsuRI GGCC 4 cut(s) 159, 251, 603, 621
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 258
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 4 cut(s) 370, 434, 438, 601
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 2 cut(s) 24, 62
Cfr10I RCCGGY 1 cut(s) 566
Cfr13I GGNCC 2 cut(s) 249, 620
Csp6I GTAC 2 cut(s) 12, 473
CviAII CATG 2 cut(s) 220, 433
CviQI GTAC 2 cut(s) 12, 473
DdeI CTNAG 3 cut(s) 56, 309, 554
DpnI GATC 2 cut(s) 95, 384
DpnII GATC 2 cut(s) 93, 382
Eam1104I CTCTTC 2 cut(s) 74, 84
EarI CTCTTC 2 cut(s) 74, 84
Eco130I CCWWGG 1 cut(s) 154
Eco147I AGGCCT 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 29
Eco47III AGCGCT 1 cut(s) 23
EcoO109I RGGNCCY 2 cut(s) 249, 620
EcoRI GAATTC 1 cut(s) 659
EcoT14I CCWWGG 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 2 cut(s) 223, 436
FaiI YATR 6 cut(s) 133, 201, 221, 408, 434, 630
FaqI GGGAC 1 cut(s) 523
FatI CATG 2 cut(s) 219, 432
Fnu4HI GCNGC 6 cut(s) 25, 106, 148, 151, 177, 564
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 6 cut(s) 25, 106, 148, 151, 177, 564
GlaI GCGC 2 cut(s) 23, 61
GluI GCNGC 6 cut(s) 25, 106, 148, 151, 177, 564
GsaI CCCAGC 1 cut(s) 183
HaeII RGCGCY 1 cut(s) 25
HaeIII GGCC 4 cut(s) 159, 251, 603, 621
HapII CCGG 1 cut(s) 567
HhaI GCGC 2 cut(s) 24, 62
Hin1II CATG 2 cut(s) 223, 436
Hin6I GCGC 2 cut(s) 22, 60
HinP1I GCGC 2 cut(s) 22, 60
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HindIII AAGCTT 1 cut(s) 204
HinfI GANTC 3 cut(s) 48, 313, 646
HpaII CCGG 1 cut(s) 567
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 3 cut(s) 127, 240, 525
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 3 cut(s) 97, 454, 526
Hpy8I GTNNAC 3 cut(s) 127, 240, 525
HpyAV CCTTC 4 cut(s) 94, 240, 587, 611
HpyCH4III ACNGT 3 cut(s) 11, 16, 31
HpyCH4V TGCA 9 cut(s) 27, 127, 269, 297, 353, 368, 563, 635, 668
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 185, 365, 374, 632
HpyF3I CTNAG 3 cut(s) 56, 309, 554
Hsp92II CATG 2 cut(s) 223, 436
HspAI GCGC 2 cut(s) 22, 60
Kzo9I GATC 2 cut(s) 93, 382
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 2 cut(s) 19, 596
Lsp1109I GCAGC 5 cut(s) 11, 117, 137, 188, 575
LweI GCATC 1 cut(s) 622
MalI GATC 2 cut(s) 95, 384
MboI GATC 2 cut(s) 93, 382
MboII GAAGA 5 cut(s) 61, 95, 98, 101, 339
MhlI GDGCHC 2 cut(s) 129, 361
MluCI AATT 2 cut(s) 273, 659
MlyI GAGTC 1 cut(s) 57
MnlI CCTC 8 cut(s) 152, 161, 188, 218, 228, 262, 451, 493
MseI TTAA 2 cut(s) 276, 542
MslI CAYNNNNRTG 1 cut(s) 530
MspA1I CMGCKG 2 cut(s) 179, 323
MspI CCGG 1 cut(s) 567
MwoI GCNNNNNNNGC 5 cut(s) 156, 185, 365, 374, 632
NdeII GATC 2 cut(s) 93, 382
NlaIII CATG 2 cut(s) 223, 436
NlaIV GGNNCC 1 cut(s) 358
NmeAIII GCCGAG 1 cut(s) 123
NspI RCATGY 1 cut(s) 436
OliI CACNNNNGTG 1 cut(s) 530
PaeI GCATGC 1 cut(s) 436
PaqCI CACCTGC 1 cut(s) 344
PceI AGGCCT 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 313, 646
PkrI GCNGC 6 cut(s) 26, 107, 149, 152, 178, 565
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PspFI CCCAGC 1 cut(s) 179
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 2 cut(s) 249, 620
PstI CTGCAG 2 cut(s) 355, 565
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 2 cut(s) 179, 323
RsaI GTAC 2 cut(s) 13, 474
RsaNI GTAC 2 cut(s) 12, 473
RseI CAYNNNNRTG 1 cut(s) 530
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 2 cut(s) 276, 542
SatI GCNGC 6 cut(s) 25, 106, 148, 151, 177, 564
Sau3AI GATC 2 cut(s) 93, 382
Sau96I GGNCC 2 cut(s) 249, 620
SchI GAGTC 1 cut(s) 57
SduI GDGCHC 2 cut(s) 129, 361
SfaNI GCATC 1 cut(s) 622
SfcI CTRYAG 2 cut(s) 351, 561
SmiMI CAYNNNNRTG 1 cut(s) 530
SphI GCATGC 1 cut(s) 436
Sse9I AATT 2 cut(s) 273, 659
SseBI AGGCCT 1 cut(s) 159
SsiI CCGC 2 cut(s) 147, 377
StuI AGGCCT 1 cut(s) 159
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 11, 16, 31
TasI AATT 2 cut(s) 273, 659
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 2 cut(s) 313, 646
Tru1I TTAA 2 cut(s) 276, 542
Tru9I TTAA 2 cut(s) 276, 542
TscAI CASTG 1 cut(s) 265
TseI GCWGC 5 cut(s) 24, 105, 150, 176, 563
TspDTI ATGAA 1 cut(s) 652
TspGWI ACGGA 2 cut(s) 127, 207
TspRI CASTG 1 cut(s) 265
VneI GTGCAC 1 cut(s) 125
XapI RAATTY 1 cut(s) 659
XceI RCATGY 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.