FvH4_4g05680

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006689
Physical Location & Seq
Reverse (-)
5001809 .. 5008702
6894 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05680.t4

Sequence Viewer

Length: 2148 bp
ATGGAGGGAGGGAGCTCCAAATTGCAGAGCATACAAGATGCCATCAGATCCTCAGATGTGGTGGAGAATCGGATTGAGTTAATTAAGAAGCTTGGTGATTTGAAAATAACAGAGAAATCTGAGATGGCTTCGCTTGCCGAGTGTTTGACAATATTCTGGGAGGACTATACTTGCTTGGATTTAAGCCAGTGCATGTTAAATAGTGCTATAATACAGGTGGCATCGAAGCACCTAGAATCGGACATATCTAACTGTCTAGCACATTTCCTTGTCCTTGGGACCAAGGCAAGCATATGGTGTGGAAAGCACCTAAAAATGACTCTTATGTCGACCGAGGAATCTCAAGAAGAAGAGCATGAGAACCTGTTCTTTCAGCTGTTTCTGGATCTGCTTAGCTTTTCGGGGGCTAGCTTTTCAGCACTGGCAAGATACCCTGTTTTTGTTGAGAAGATGACAGTGGATATAGTTGAAACCTTTTTTGATGAAGAGTTAAACTTGATCAAAGATTCATTACCAGAGATAAAGAGAATTCAGTCATTTGTATCAGAAGAAGTAGTTGTAAAGGTCACACAGGAGGTCATTGATTCTGTAATTAGACTGTGTGGAACATATGCCCGAGCTTTGAATTGGGAGTCCTGGGAAGAAAAACTCGAGAGGGACAAAACTGACATGGACTTTCAAGGGGCCAAAAGTATGAATCATGTTATCAATGTAACAAAGTGTACCGTAGAAAAACTATGTGAGATGGGAGTTGTTGCAGCAAATAATGGAGGAAGTCTAGTACCAATCATTAATATGTCTTGGAAAGGTGTGGTTACTTTGCTTCAGCTTGGTGATGGTGTATTAGCAACAAAGGTGAACATAGCAGTTCTAATTTCAAATCTAATCTCATTGGTTAATGAATCTATAAGACGTGCTGCTGAGGCTTGGTTTTCATCACTGAAGGAAAACATTTCTGTGACAGAAGCTAGAAAGGCGTTTCTTCCTGTAAAGTTTTATTTGGTTACTGCAGTGAAAATATCTTCCCTATACCCATATCAAGCATATCTGGTGCGAAGTGAGATAACAAAATGTATCCTGATGATGTCAATGTTAAAGTGTTCACTGAGCAAAGAAAATCTGTTAAAAACTGCCAGTGATGTCTTCACAGAACTCTTGGAGAAGACATCCTTGGATTTGCTTATTGCTATGCTGAACTCAGTTCAAGTAAAGCAGGAGCTGAAGGTTGAGTTTCTAGACTATCTCTTCAGTGAAGGAAGCTGCACAGATTCTGTCGCTGGAGATCCAAGTAAGATGCATATAATGGTTGACTTATTTTCTACATGTGGGGAAGTATTGCCTGGAGAAAGAACCTTATTGATTGGCCATATTTCATTGTTTCTTAATTTTCTGAGATTTTCTCTTGATCTTGACGATGATGTAAAGCTTTGGATTGCTGGAAAGCTTGATTGGTTCTTTGATATGTTGGTAAATGAAGAGGTATATTCTTCCATTCTTCTCCTGCAAGTTCCTGTGCTATATGGTTGTGGAAAAACTGTGGAAGTTGTCTGGCAGCCTATGTTTTCTTCTCTTTTGAATGCATTGCAAACCTTCATGCTTGTGGTTTCTTCTAATCCTGCTTGGACGGAGTTGGAGTCTTTCCTTCTTGAGAATTTATTTCACCCCCACTTTCTTTGCTGGGAGATTGTCATGGAACTTTGGTGCTTTATGGTTCGCCATGCTGAATCAGGGATGGTGAGTGGCATTGTTGGTAAACTTTGCTCATTAATGAAGTTGGTGGCATCTACTGAATCAGTTCTTATCGCTGATTCTGCTCTGAGAAAAGTTGCAAGGTCAATCAGCACAATTCTTACTTTTGGCGCACAATCTATGGTAGACCAGGTTTACATGGCCATTGTCCGTGATGAGGGAACCCAACTGTCATCAGTTATGCGTCTGGCGCTGTTCATGGAGGGCTTCCCACTGAATTTACTGTCAGACAAGATGAAAAGAATTGTGACGCAAAGGATAATGTCTGATTACTGTCTCTTCATTGAAAATTTTGATGAGAAGTCAATGAACTCTCTTGACTGTGGTCTTTTCGGGGCCCCAGTTTTTGCTTTGTCATCTTCGTTGCAATCTCTATCAACACATCTGATATCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

716

Amino Acids

80.21

Weight (kDa)

4.92

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 222 - 709 9.2e-115 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 325
AccI GTMKAC 2 cut(s) 329, 1875
AclWI GGATC 3 cut(s) 42, 393, 1277
AcoI YGGCCR 2 cut(s) 1363, 1890
AcsI RAATTY 4 cut(s) 528, 1651, 1966, 2038
AcuI CTGAAG 4 cut(s) 809, 962, 1231, 1241
AfaI GTAC 2 cut(s) 724, 783
AfiI CCNNNNNNNGG 2 cut(s) 238, 1906
AflIII ACRYGT 1 cut(s) 1322
AgsI TTSAA 8 cut(s) 103, 470, 625, 680, 879, 1205, 1576, 2036
AjiI CACGTC 1 cut(s) 914
AjnI CCWGG 3 cut(s) 635, 1339, 1878
AjuI GAANNNNNNNTTGG 4 cut(s) 1154, 1186, 1432, 1464
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 2030
AlwI GGATC 3 cut(s) 42, 393, 1277
AlwNI CAGNNNCTG 2 cut(s) 1219, 1271
Ama87I CYCGRG 2 cut(s) 615, 650
AoxI GGCC 4 cut(s) 684, 1363, 1890, 2085
ApaI GGGCCC 1 cut(s) 2089
ApeKI GCWGC 4 cut(s) 758, 917, 1260, 1552
ApoI RAATTY 4 cut(s) 528, 1651, 1966, 2038
AseI ATTAAT 2 cut(s) 792, 1766
Asp700I GAANNNNTTC 2 cut(s) 365, 1794
AspLEI GCGC 2 cut(s) 1862, 1942
AspS9I GGNCC 4 cut(s) 279, 684, 2085, 2086
AsuHPI GGTGA 5 cut(s) 107, 845, 868, 1652, 1747
AsuNHI GCTAGC 1 cut(s) 407
AvaI CYCGRG 2 cut(s) 615, 650
AvaII GGWCC 1 cut(s) 279
BaeGI GKGCMC 1 cut(s) 2089
BalI TGGCCA 2 cut(s) 1365, 1892
BanII GRGCYC 2 cut(s) 17, 2089
BbsI GAAGAC 2 cut(s) 1135, 1169
Bbv12I GWGCWC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 4 cut(s) 770, 904, 1247, 1564
BccI CCATC 5 cut(s) 50, 118, 739, 830, 1726
BciT130I CCWGG 3 cut(s) 637, 1341, 1880
BciVI GTATCC 1 cut(s) 1085
BclI TGATCA 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 2030
BfaI CTAG 6 cut(s) 233, 257, 408, 779, 969, 1235
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 1 cut(s) 1943
BfuI GTATCC 1 cut(s) 1085
BisI GCNGC 4 cut(s) 759, 918, 1261, 1553
BlpI GCTNAGC 1 cut(s) 392
BlsI GCNGC 4 cut(s) 760, 919, 1262, 1554
Bme1390I CCNGG 3 cut(s) 637, 1341, 1880
Bme18I GGWCC 1 cut(s) 279
BmeT110I CYCGRG 2 cut(s) 615, 650
BmgBI CACGTC 1 cut(s) 914
BmgT120I GGNCC 4 cut(s) 279, 684, 2085, 2086
BmiI GGNNCC 6 cut(s) 280, 685, 1912, 2086, 2087, 2088
BmrFI CCNGG 3 cut(s) 637, 1341, 1880
BmrI ACTGGG 1 cut(s) 2084
BmsI GCATC 4 cut(s) 28, 230, 1284, 1790
BmtI GCTAGC 1 cut(s) 411
BmuI ACTGGG 1 cut(s) 2084
BoxI GACNNNNGTC 1 cut(s) 2073
BpiI GAAGAC 2 cut(s) 1135, 1169
BplI GAGNNNNNCTC 2 cut(s) 1384, 1416
BpmI CTGGAG 2 cut(s) 1299, 1362
Bpu10I CCTNAGC 1 cut(s) 921
Bpu1102I GCTNAGC 1 cut(s) 392
BpuEI CTTGAG 2 cut(s) 327, 1667
BsaJI CCNNGG 5 cut(s) 274, 282, 333, 636, 1170
BsaXI ACNNNNNCTCC 2 cut(s) 1619, 1649
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 1906
Bse1I ACTGG 4 cut(s) 187, 426, 1134, 2090
Bse3DI GCAATG 1 cut(s) 1580
BseBI CCWGG 3 cut(s) 637, 1341, 1880
BseDI CCNNGG 5 cut(s) 274, 282, 333, 636, 1170
BseGI GGATG 2 cut(s) 1166, 1737
BseLI CCNNNNNNNGG 2 cut(s) 238, 1906
BseMI GCAATG 1 cut(s) 1580
BseMII CTCAG 7 cut(s) 66, 111, 912, 1097, 1212, 1382, 1808
BseNI ACTGG 4 cut(s) 187, 426, 1134, 2090
BseSI GKGCMC 1 cut(s) 2089
BseXI GCAGC 4 cut(s) 770, 904, 1247, 1564
BseYI CCCAGC 1 cut(s) 1677
BsgI GTGCAG 1 cut(s) 1246
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 4 cut(s) 686, 1365, 1892, 2087
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 2 cut(s) 615, 650
BslFI GGGAC 2 cut(s) 292, 671
BslI CCNNNNNNNGG 2 cut(s) 238, 1906
BsmAI GTCTC 1 cut(s) 2030
BsmFI GGGAC 2 cut(s) 292, 671
BsmI GAATGC 1 cut(s) 1582
BsnI GGCC 4 cut(s) 686, 1365, 1892, 2087
BsoBI CYCGRG 2 cut(s) 615, 650
Bsp120I GGGCCC 1 cut(s) 2085
Bsp1286I GDGCHC 2 cut(s) 17, 2089
Bsp143I GATC 5 cut(s) 47, 385, 498, 1282, 1405
Bsp1720I GCTNAGC 1 cut(s) 392
BspANI GGCC 4 cut(s) 686, 1365, 1892, 2087
BspCNI CTCAG 7 cut(s) 65, 112, 913, 1098, 1211, 1383, 1809
BspLI GGNNCC 6 cut(s) 280, 685, 1912, 2086, 2087, 2088
BspMAI CTGCAG 1 cut(s) 1012
BspOI GCTAGC 1 cut(s) 411
BspPI GGATC 3 cut(s) 42, 393, 1277
BspQI GCTCTTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 1580
BsrI ACTGG 4 cut(s) 187, 426, 1134, 2090
BssECI CCNNGG 5 cut(s) 274, 282, 333, 636, 1170
BssMI GATC 5 cut(s) 47, 385, 498, 1282, 1405
BssT1I CCWWGG 3 cut(s) 274, 282, 1170
Bst2UI CCWGG 3 cut(s) 637, 1341, 1880
Bst4CI ACNGT 9 cut(s) 254, 457, 600, 727, 1537, 1920, 1974, 2024, 2072
Bst6I CTCTTC 5 cut(s) 345, 480, 1250, 1470, 2033
BstC8I GCNNGC 3 cut(s) 135, 289, 409
BstDEI CTNAG 8 cut(s) 52, 120, 392, 921, 1106, 1198, 1391, 1817
BstF5I GGATG 2 cut(s) 1166, 1737
BstH2I RGCGCY 1 cut(s) 1943
BstHHI GCGC 2 cut(s) 1862, 1942
BstKTI GATC 5 cut(s) 50, 388, 501, 1285, 1408
BstMAI GTCTC 1 cut(s) 2030
BstMBI GATC 5 cut(s) 47, 385, 498, 1282, 1405
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 5 cut(s) 134, 923, 974, 1811, 1939
BstNI CCWGG 3 cut(s) 637, 1341, 1880
BstNSI RCATGY 2 cut(s) 196, 1326
BstPAI GACNNNNGTC 1 cut(s) 2073
BstSCI CCNGG 3 cut(s) 635, 1339, 1878
BstSFI CTRYAG 1 cut(s) 1008
BstSLI GKGCMC 1 cut(s) 2089
BstV1I GCAGC 4 cut(s) 770, 904, 1247, 1564
BstV2I GAAGAC 2 cut(s) 1135, 1169
BstX2I RGATCY 3 cut(s) 47, 385, 1282
BstYI RGATCY 3 cut(s) 47, 385, 1282
BsuI GTATCC 1 cut(s) 1085
BsuRI GGCC 4 cut(s) 686, 1365, 1892, 2087
BtrI CACGTC 1 cut(s) 914
BtsCI GGATG 2 cut(s) 1166, 1737
BtsI GCAGTG 1 cut(s) 1017
BtsIMutI CAGTG 9 cut(s) 194, 419, 462, 938, 1017, 1103, 1141, 1255, 1961
Cac8I GCNNGC 3 cut(s) 135, 289, 409
CaiI CAGNNNCTG 2 cut(s) 1219, 1271
CfoI GCGC 2 cut(s) 1862, 1942
Cfr13I GGNCC 4 cut(s) 279, 684, 2085, 2086
CseI GACGC 2 cut(s) 1922, 2008
CsiI ACCWGGT 1 cut(s) 1878
Csp6I GTAC 2 cut(s) 723, 782
CspCI CAANNNNNGTGG 2 cut(s) 1655, 1690
CviQI GTAC 2 cut(s) 723, 782
DdeI CTNAG 8 cut(s) 52, 120, 392, 921, 1106, 1198, 1391, 1817
DpnI GATC 5 cut(s) 49, 387, 500, 1284, 1407
DpnII GATC 5 cut(s) 47, 385, 498, 1282, 1405
DrdI GACNNNNNNGTC 1 cut(s) 325
DseDI GACNNNNNNGTC 1 cut(s) 325
EaeI YGGCCR 2 cut(s) 1363, 1890
Eam1104I CTCTTC 5 cut(s) 345, 480, 1250, 1470, 2033
EarI CTCTTC 5 cut(s) 345, 480, 1250, 1470, 2033
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 3 cut(s) 274, 282, 1170
Eco24I GRGCYC 2 cut(s) 17, 2089
Eco32I GATATC 1 cut(s) 2139
Eco47I GGWCC 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 809, 962, 1231, 1241
Eco88I CYCGRG 2 cut(s) 615, 650
EcoICRI GAGCTC 1 cut(s) 15
EcoO109I RGGNCCY 2 cut(s) 2085, 2086
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 3 cut(s) 635, 1339, 1878
EcoRV GATATC 1 cut(s) 2139
EcoT14I CCWWGG 3 cut(s) 274, 282, 1170
EcoT22I ATGCAT 2 cut(s) 1299, 1582
EcoT38I GRGCYC 2 cut(s) 17, 2089
ErhI CCWWGG 3 cut(s) 274, 282, 1170
FalI AAGNNNNNCTT 2 cut(s) 1154, 1186
FaqI GGGAC 2 cut(s) 292, 671
FauNDI CATATG 2 cut(s) 293, 610
FbaI TGATCA 1 cut(s) 498
FblI GTMKAC 2 cut(s) 329, 1875
Fnu4HI GCNGC 4 cut(s) 759, 918, 1261, 1553
FokI GGATG 2 cut(s) 1153, 1744
FriOI GRGCYC 2 cut(s) 17, 2089
Fsp4HI GCNGC 4 cut(s) 759, 918, 1261, 1553
FspBI CTAG 6 cut(s) 233, 257, 408, 779, 969, 1235
GlaI GCGC 2 cut(s) 1861, 1941
GluI GCNGC 4 cut(s) 759, 918, 1261, 1553
GsaI CCCAGC 1 cut(s) 1681
GsuI CTGGAG 2 cut(s) 1299, 1362
HaeII RGCGCY 1 cut(s) 1943
HaeIII GGCC 4 cut(s) 686, 1365, 1892, 2087
HgaI GACGC 2 cut(s) 1922, 2008
HhaI GCGC 2 cut(s) 1862, 1942
Hin6I GCGC 2 cut(s) 1860, 1940
HinP1I GCGC 2 cut(s) 1860, 1940
HincII GTYRAC 2 cut(s) 330, 1309
HindII GTYRAC 2 cut(s) 330, 1309
HindIII AAGCTT 3 cut(s) 89, 1424, 1442
HphI GGTGA 5 cut(s) 107, 845, 868, 1652, 1747
Hpy166II GTNNAC 8 cut(s) 330, 723, 859, 1103, 1309, 1754, 1876, 1885
Hpy188III TCNNGA 9 cut(s) 344, 383, 652, 1078, 1235, 1403, 1409, 1646, 2066
Hpy8I GTNNAC 8 cut(s) 330, 723, 859, 1103, 1309, 1754, 1876, 1885
HpyAV CCTTC 5 cut(s) 937, 1216, 1247, 1600, 1652
HpyCH4III ACNGT 9 cut(s) 254, 457, 600, 727, 1537, 1920, 1974, 2024, 2072
HpyCH4IV ACGT 1 cut(s) 913
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 923, 974, 1811, 1939
HpyF3I CTNAG 8 cut(s) 52, 120, 392, 921, 1106, 1198, 1391, 1817
HpySE526I ACGT 1 cut(s) 913
HspAI GCGC 2 cut(s) 1860, 1940
Ksp22I TGATCA 1 cut(s) 498
Kzo9I GATC 5 cut(s) 47, 385, 498, 1282, 1405
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 3 cut(s) 12, 20, 1216
Lsp1109I GCAGC 4 cut(s) 770, 904, 1247, 1564
LweI GCATC 4 cut(s) 28, 230, 1284, 1790
MabI ACCWGGT 1 cut(s) 1878
MaeI CTAG 6 cut(s) 233, 257, 408, 779, 969, 1235
MaeII ACGT 1 cut(s) 913
MaeIII GTNAC 6 cut(s) 565, 712, 814, 958, 1003, 1996
MalI GATC 5 cut(s) 49, 387, 500, 1284, 1407
MboI GATC 5 cut(s) 47, 385, 498, 1282, 1405
MflI RGATCY 3 cut(s) 47, 385, 1282
MhlI GDGCHC 2 cut(s) 17, 2089
MlsI TGGCCA 2 cut(s) 1365, 1892
MluNI TGGCCA 2 cut(s) 1365, 1892
MlyI GAGTC 3 cut(s) 313, 641, 1643
MmeI TCCRAC 1 cut(s) 1611
Mox20I TGGCCA 2 cut(s) 1365, 1892
Mph1103I ATGCAT 2 cut(s) 1299, 1582
MroXI GAANNNNTTC 2 cut(s) 365, 1794
MscI TGGCCA 2 cut(s) 1365, 1892
MslI CAYNNNNRTG 3 cut(s) 794, 956, 1598
Msp20I TGGCCA 2 cut(s) 1365, 1892
MspA1I CMGCKG 1 cut(s) 376
MspR9I CCNGG 3 cut(s) 637, 1341, 1880
Mva1269I GAATGC 1 cut(s) 1582
MvaI CCWGG 3 cut(s) 637, 1341, 1880
MwoI GCNNNNNNNGC 5 cut(s) 134, 923, 974, 1811, 1939
NdeI CATATG 2 cut(s) 293, 610
NdeII GATC 5 cut(s) 47, 385, 498, 1282, 1405
NheI GCTAGC 1 cut(s) 407
NlaIV GGNNCC 6 cut(s) 280, 685, 1912, 2086, 2087, 2088
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 3 cut(s) 565, 958, 1996
NsiI ATGCAT 2 cut(s) 1299, 1582
NspI RCATGY 2 cut(s) 196, 1326
PacI TTAATTAA 1 cut(s) 84
PaeR7I CTCGAG 1 cut(s) 650
PciI ACATGT 1 cut(s) 1322
PciSI GCTCTTC 1 cut(s) 345
PctI GAATGC 1 cut(s) 1582
PdmI GAANNNNTTC 2 cut(s) 365, 1794
PkrI GCNGC 4 cut(s) 760, 919, 1262, 1554
PleI GAGTC 3 cut(s) 313, 640, 1642
PpsI GAGTC 3 cut(s) 313, 640, 1642
PscI ACATGT 1 cut(s) 1322
PshAI GACNNNNGTC 1 cut(s) 2073
PshBI ATTAAT 2 cut(s) 792, 1766
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 635, 1339, 1878
PspFI CCCAGC 1 cut(s) 1677
PspGI CCWGG 3 cut(s) 635, 1339, 1878
PspN4I GGNNCC 6 cut(s) 280, 685, 1912, 2086, 2087, 2088
PspOMI GGGCCC 1 cut(s) 2085
PspPI GGNCC 4 cut(s) 279, 684, 2085, 2086
PstI CTGCAG 1 cut(s) 1012
PstNI CAGNNNCTG 2 cut(s) 1219, 1271
PsuI RGATCY 3 cut(s) 47, 385, 1282
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 2 cut(s) 724, 783
RsaNI GTAC 2 cut(s) 723, 782
RseI CAYNNNNRTG 3 cut(s) 794, 956, 1598
SacI GAGCTC 1 cut(s) 17
SalI GTCGAC 1 cut(s) 328
SapI GCTCTTC 1 cut(s) 345
SatI GCNGC 4 cut(s) 759, 918, 1261, 1553
Sau3AI GATC 5 cut(s) 47, 385, 498, 1282, 1405
Sau96I GGNCC 4 cut(s) 279, 684, 2085, 2086
SchI GAGTC 3 cut(s) 313, 641, 1643
ScrFI CCNGG 3 cut(s) 637, 1341, 1880
SduI GDGCHC 2 cut(s) 17, 2089
SexAI ACCWGGT 1 cut(s) 1878
SfaNI GCATC 4 cut(s) 28, 230, 1284, 1790
SfcI CTRYAG 1 cut(s) 1008
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 1 cut(s) 279
SlaI CTCGAG 1 cut(s) 650
SmiMI CAYNNNNRTG 3 cut(s) 794, 956, 1598
SmlI CTYRAG 3 cut(s) 342, 650, 1646
SmoI CTYRAG 3 cut(s) 342, 650, 1646
SspI AATATT 1 cut(s) 153
SspMI CTAG 6 cut(s) 233, 257, 408, 779, 969, 1235
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 3 cut(s) 635, 1339, 1878
StyI CCWWGG 3 cut(s) 274, 282, 1170
TaaI ACNGT 9 cut(s) 254, 457, 600, 727, 1537, 1920, 1974, 2024, 2072
TaiI ACGT 1 cut(s) 916
TaqI TCGA 3 cut(s) 224, 329, 651
TaqII GACCGA 1 cut(s) 347
TscAI CASTG 9 cut(s) 194, 426, 462, 945, 1017, 1110, 1141, 1255, 1968
TseFI GTSAC 3 cut(s) 565, 958, 1996
TseI GCWGC 4 cut(s) 758, 917, 1260, 1552
Tsp45I GTSAC 3 cut(s) 565, 958, 1996
TspGWI ACGGA 2 cut(s) 1640, 1889
TspRI CASTG 9 cut(s) 194, 426, 462, 945, 1017, 1110, 1141, 1255, 1968
VpaK11BI GGWCC 1 cut(s) 279
VspI ATTAAT 2 cut(s) 792, 1766
XapI RAATTY 4 cut(s) 528, 1651, 1966, 2038
XbaI TCTAGA 1 cut(s) 1234
XceI RCATGY 2 cut(s) 196, 1326
XhoI CTCGAG 1 cut(s) 650
XmiI GTMKAC 2 cut(s) 329, 1875
XmnI GAANNNNTTC 2 cut(s) 365, 1794
XspI CTAG 6 cut(s) 233, 257, 408, 779, 969, 1235
Zsp2I ATGCAT 2 cut(s) 1299, 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.