Rw4G005980

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006689
Physical Location & Seq
Reverse (-)
12100417 .. 12107129
6713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005980.1

Sequence Viewer

Length: 3063 bp
ATGGAGGGAGGGAGCTCAGCATTGCAGAGCATACAAGACGCCATCAGATCCTCAGATGTGGTGGAGAATCGGATTGAATTACTTAAGAAGCTTGGCGATTTGAAAATAACAGAGAAGTCTGAATTGGCTTCCCTTGCCGAGTGTTTGACAATACTTATTTGGTCCATTGTTTTGCACTTAACAACAAGCTCGATATTCTGGGAGGAGTATACTTGCTTGGATTTAAGCCAGTGCATGTTAAATAGCGCTATATTACAGGTGGCTGCGAAGCACCTAGAATCAGACACATCCAACTGTCTAGCACATTTCCTTGTCCTTGGGACCAAGGCAAGCATATGGTGTGGAAAGCACCTAAAAATGACTCTTATGTCAACCGAGGAATCTCAAGAAGAAGAGCACGAGAACCTGTTCTTTCAGCTGTTTCTGGATCTGCTGAGCTTTTTGAGGGCTTGCTTTTCAGCTTTGGCAAGATACCCTGTTTCTGTTGAGAAGATGACAGTGGATGTAGTTGAAACATTCTTTGATGAAGAGTTAAACTTGATCAAAGATTCTATATCAGAGATAAAGAGAATTCAGTCATTTGTATCAGAAGAAGTCGTAGTAAAGGTTACACAGGAGGTCATTGATTCTGTAATAAGATTGTGTGGAGCATATGCCCGAGCTTTGAATTGGGAGTCTTGGGAAGAAAAACTCGAGAGGGATAAAACTGACATGGACTTTGAAGGGGCCAATAGTATGAATCATGTTATTAATGTAACAAAGTGTACAATAGAAAAACTATGTGAGATGGGCATTATTGCAGCTAATAATGGCGGAAGTCTGGTACCGATTATTAATATGTCGTGGAAAGGTGTGGTCACTTTGCTTCAGCTCGGTGATGGGGTATTAGCAACAAAGGTGAATGTAGCAGCTCTAATATCAAATCTAATCTCATTGGTTAATGAATCTATAAGACATGCAGCTGAGGCATGGTTTTCATCACTGAAGGAAAACATCTCTGTGTCAGAAGCTAGAAAGGCATTTCTTCCTATAAAGTTTTATTTGGTTAATGCAGTAAAAATATCTTCCCTATACCCATATCAAGCATATCTGGTGCAAAGTGAGATAACAAAATGTATCCTGATGATGTCAACCTTAAAGATTTCATTGAGCAATGAAAACCTGTTAAAAACTGCCAGTGATGTTTTCACAGAACTCTTGGAGAAGACATCCTTGGATTTGCTTATTGCTCTACTGAACTCAGTTCAAGTGAAGCGGGAGCTGAAGGTTGAGATTCTAGACTATCTCTTCGGTGAAGGAAGCTGCACAAATTCTGTTGATGGAGATCCGACTACATGTGGGGAAGCATTGCCCGGAGAAAGGACCTTCTTGATTGGCCGTGTTTCATTGTTTCTTAATTTTCTGAGATTTTCTCTCGATCTTGACGATGATGTAAAGCTTGGGATTGCTGGAAAGCTTGGTTGGTTCTTTGATATGTTAGTAAATGAAGAGGTATATTCTTCCATTCTTCTTTTGCAAGTTCCTGTGCTATATGGTTCTGGAAAAACTGTGGAAGTTGTCTGGCAACCTTTGTTTTCTTCTCTTTTGAATGCATTGCAAATCTACATGCTTGTCGTTTCTTCTGGTCCTGCTTGGAGGGAGCTGGAGTCTTTTCTTCTTGAGAATTTATTTCACCCCCACTTTCTTTGCTGGGAGATTGTAATGGAACTTTGGTGCTTTATGGTTCGCCATGCTGAGTCTGGGATGGTGAGTGGCATTGCTGGTAAACTTTGCTCATTAATGAAGTTGGTGGCATCTACTGAATCCGTTCTTGTAGCTGATTCTGCTCTGAGAAAAGTGGCAAGGTCAATCAGCATAATTCTTACTTTTGGTGCACAATCTATGGTAGACCAGGTTTACATGGCCATTGTACGTGATGAGGGAACCCAATTGTCATCAGTTATGCGTCTGGCGTTGTTCATGGAGGGCTTCCCACTGAATTTACTGTCAGACAAGATGAAAAGAATTGCGACGCAAAGGATAATTACTGATTACTGTCTCTTCGTTGAAAATTTTGATGAGAAGTCAATGAAATCTCTTGATTGTGGTCTTTTTGGGGCCCCAGTTTTTGCTTTGTCAGCTTCGTTGCAGTCTCTTCAGATCAGCACATCTGATATCAATGTGAAGACCTTGAAGTTTTTAGTTGCCATCATTCATAACTACAGAGTTTGTGAAGATCAGCTAATGAAGGACCAGTACCGTAAGCTTCTGAGTGAAACATTAGTGATCATCTCAAATATGAAGCACCTATATGCATCTCTTGAAATGGAGAAAGTAATCTGTGAGCTTGAAAACCTGTTTATCTCGGCACCTGCTGTTTCTGATACCCAATTGTATGAATGCAAACCAGATCTGGCTCTTTTCATGGCAGGCCTTGCTCACATGGATATCAAAGAAGCTGATGAAAGTTCCAAGATCTCTGCTCTATGGGATTTGTATCACATGTTATTGGGAGAGCGGCACTGGGCATTTATTCATTTGGCAATTACAGCATTTGGATACTTTTCTGCTCGGACAAATTGCAATAAGCTCTGGAGATTCGTGCCTCAGAATGCAGCGCTTTCCTATGATCTGACTTCTGGAAATGAGGCCAATCTGGAGCGATTTATGTCTGAGTTCAAGGTTTTTCTTGAGAAGGAAACGGCACTTCTCACAATAACGGCTAGCTCTGACCAGCTTGGTCTGCTTGTGAGGGAGGGTCTAAAACTAAAAGAAATGTTCCAAAAGATTTCAAAAGTCGTCGTTGAGACTGCGGAATGTGACTACATGGAAATTGATGGTGAAAAGCAGGCCATGGAGATGAACGGTGGAAAACAATCCAATAGGAAAAGGAAATTTCCTGATGGAATTGAAAAGGGAATGGAACTGCTACAAAGTGGTATGAAGGTTATTGTGGATAGTATTTCTCAGTTGCAGCAAAATGATCAACTCAGTCCCAATGAACTACCCCACAACTTCTTGACCACCTTTTCTCGCCTTGAAGCTGAAATTTCTCAACTAGTTGGCCTGGCTGGTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

1020

Amino Acids

114.3

Weight (kDa)

5.0

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 277 - 857 2.4e-149 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2359
AasI GACNNNNNNGTC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 2359
Acc65I GGTACC 1 cut(s) 823
AccB1I GGYRCC 2 cut(s) 823, 2347
AccBSI CCGCTC 1 cut(s) 2497
AccI GTMKAC 2 cut(s) 209, 1887
AciI CCGC 4 cut(s) 813, 1255, 2497, 2792
AclWI GGATC 3 cut(s) 42, 435, 1319
AcoI YGGCCR 2 cut(s) 1375, 1902
AcsI RAATTY 7 cut(s) 570, 1309, 1663, 1978, 2050, 2873, 3027
AcuI CTGAAG 4 cut(s) 851, 1004, 1283, 2120
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 4 cut(s) 766, 825, 1911, 2237
AfeI AGCGCT 2 cut(s) 247, 2598
AfiI CCNNNNNNNGG 1 cut(s) 1359
AflII CTTAAG 1 cut(s) 83
AflIII ACRYGT 2 cut(s) 1334, 2481
AhlI ACTAGT 1 cut(s) 3037
AjnI CCWGG 2 cut(s) 1890, 3045
AjuI GAANNNNNNNTTGG 6 cut(s) 107, 139, 1196, 1228, 1444, 1476
Alw21I GWGCWC 3 cut(s) 17, 399, 1876
Alw26I GTCTC 3 cut(s) 2042, 2136, 2780
Alw44I GTGCAC 1 cut(s) 1872
AlwI GGATC 3 cut(s) 42, 435, 1319
Ama87I CYCGRG 2 cut(s) 657, 692
Aor51HI AGCGCT 2 cut(s) 247, 2598
AoxI GGCC 8 cut(s) 726, 1375, 1902, 2097, 2410, 2628, 2829, 3043
ApaI GGGCCC 1 cut(s) 2101
ApaLI GTGCAC 1 cut(s) 1872
ApeKI GCWGC 7 cut(s) 263, 800, 908, 959, 1302, 2594, 2953
ApoI RAATTY 7 cut(s) 570, 1309, 1663, 1978, 2050, 2873, 3027
ArsI GACNNNNNNTTYG 4 cut(s) 701, 733, 1271, 1303
AseI ATTAAT 3 cut(s) 750, 834, 1778
Asp700I GAANNNNTTC 2 cut(s) 407, 1806
Asp718I GGTACC 1 cut(s) 823
AspLEI GCGC 2 cut(s) 248, 2599
AspS9I GGNCC 8 cut(s) 162, 321, 726, 1362, 1625, 2097, 2098, 2230
AsuC2I CCSGG 1 cut(s) 1353
AsuHPI GGTGA 6 cut(s) 887, 910, 1304, 1664, 1759, 2831
AsuNHI GCTAGC 1 cut(s) 2702
AvaI CYCGRG 2 cut(s) 657, 692
AvaII GGWCC 5 cut(s) 162, 321, 1362, 1625, 2230
BaeGI GKGCMC 2 cut(s) 1876, 2101
BalI TGGCCA 1 cut(s) 1904
BanI GGYRCC 2 cut(s) 823, 2347
BanII GRGCYC 2 cut(s) 17, 2101
BarI GAAGNNNNNNTAC 2 cut(s) 2219, 2251
BauI CACGAG 1 cut(s) 398
BbsI GAAGAC 2 cut(s) 1211, 2171
Bbv12I GWGCWC 3 cut(s) 17, 399, 1876
BbvCI CCTCAGC 1 cut(s) 963
BbvI GCAGC 7 cut(s) 250, 812, 920, 971, 1289, 2606, 2965
BccI CCATC 8 cut(s) 50, 781, 872, 1313, 1738, 2195, 2810, 2876
BceAI ACGGC 3 cut(s) 1362, 2697, 2715
BciT130I CCWGG 2 cut(s) 1892, 3047
BciVI GTATCC 2 cut(s) 1127, 2531
BclI TGATCA 3 cut(s) 540, 2265, 2962
BcnI CCSGG 1 cut(s) 1353
BcoDI GTCTC 3 cut(s) 2042, 2136, 2780
BcuI ACTAGT 1 cut(s) 3037
BfaI CTAG 6 cut(s) 275, 299, 1011, 1277, 2703, 3038
BfmI CTRYAG 1 cut(s) 2200
BfoI RGCGCY 2 cut(s) 249, 2600
BfrI CTTAAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 2359
BfuI GTATCC 2 cut(s) 1127, 2531
BglII AGATCT 2 cut(s) 2389, 2454
BisI GCNGC 8 cut(s) 264, 801, 909, 960, 1303, 2498, 2595, 2954
BlpI GCTNAGC 2 cut(s) 16, 434
BlsI GCNGC 8 cut(s) 265, 802, 910, 961, 1304, 2499, 2596, 2955
Bme1390I CCNGG 3 cut(s) 1353, 1892, 3047
Bme18I GGWCC 5 cut(s) 162, 321, 1362, 1625, 2230
BmeT110I CYCGRG 2 cut(s) 657, 692
BmgT120I GGNCC 8 cut(s) 162, 321, 726, 1362, 1625, 2097, 2098, 2230
BmiI GGNNCC 8 cut(s) 322, 727, 825, 1924, 2098, 2099, 2100, 2349
BmrFI CCNGG 3 cut(s) 1353, 1892, 3047
BmrI ACTGGG 2 cut(s) 2096, 2512
BmsI GCATC 2 cut(s) 1802, 2303
BmtI GCTAGC 1 cut(s) 2706
BmuI ACTGGG 2 cut(s) 2096, 2512
BpiI GAAGAC 2 cut(s) 1211, 2171
BplI GAGNNNNNCTC 2 cut(s) 1396, 1428
BpmI CTGGAG 3 cut(s) 1664, 2593, 2657
Bpu10I CCTNAGC 1 cut(s) 963
Bpu1102I GCTNAGC 2 cut(s) 16, 434
BpuEI CTTGAG 3 cut(s) 369, 1679, 2690
BpuMI CCSGG 1 cut(s) 1353
BsaAI YACGTR 1 cut(s) 1913
BsaBI GATNNNNATC 2 cut(s) 1323, 2475
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 5 cut(s) 316, 324, 375, 1212, 2832
BsaXI ACNNNNNCTCC 2 cut(s) 1631, 1661
Bsc4I CCNNNNNNNGG 1 cut(s) 1359
Bse1I ACTGG 5 cut(s) 229, 1176, 2102, 2233, 2507
Bse3DI GCAATG 5 cut(s) 20, 1159, 1346, 1592, 1755
Bse8I GATNNNNATC 2 cut(s) 1323, 2475
BseBI CCWGG 2 cut(s) 1892, 3047
BseDI CCNNGG 5 cut(s) 316, 324, 375, 1212, 2832
BseGI GGATG 4 cut(s) 287, 508, 1208, 1749
BseJI GATNNNNATC 2 cut(s) 1323, 2475
BseLI CCNNNNNNNGG 1 cut(s) 1359
BseMI GCAATG 5 cut(s) 20, 1159, 1346, 1592, 1755
BseNI ACTGG 5 cut(s) 229, 1176, 2102, 2233, 2507
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 2 cut(s) 1876, 2101
BseXI GCAGC 7 cut(s) 250, 812, 920, 971, 1289, 2606, 2965
BseYI CCCAGC 1 cut(s) 1689
BsgI GTGCAG 1 cut(s) 1288
BshFI GGCC 8 cut(s) 728, 1377, 1904, 2099, 2412, 2630, 2831, 3045
BshNI GGYRCC 2 cut(s) 823, 2347
BsiHKAI GWGCWC 3 cut(s) 17, 399, 1876
BsiHKCI CYCGRG 2 cut(s) 657, 692
BsiSI CCGG 1 cut(s) 1353
BslFI GGGAC 2 cut(s) 334, 2958
BslI CCNNNNNNNGG 1 cut(s) 1359
BsmAI GTCTC 3 cut(s) 2042, 2136, 2780
BsmFI GGGAC 2 cut(s) 334, 2958
BsmI GAATGC 3 cut(s) 1594, 2384, 2596
BsnI GGCC 8 cut(s) 728, 1377, 1904, 2099, 2412, 2630, 2831, 3045
BsoBI CYCGRG 2 cut(s) 657, 692
Bsp120I GGGCCC 1 cut(s) 2097
Bsp1286I GDGCHC 4 cut(s) 17, 399, 1876, 2101
Bsp1407I TGTACA 1 cut(s) 764
Bsp1720I GCTNAGC 2 cut(s) 16, 434
Bsp19I CCATGG 1 cut(s) 2832
BspACI CCGC 4 cut(s) 813, 1255, 2497, 2792
BspANI GGCC 8 cut(s) 728, 1377, 1904, 2099, 2412, 2630, 2831, 3045
BspLI GGNNCC 8 cut(s) 322, 727, 825, 1924, 2098, 2099, 2100, 2349
BspMI ACCTGC 1 cut(s) 2359
BspOI GCTAGC 1 cut(s) 2706
BspPI GGATC 3 cut(s) 42, 435, 1319
BspQI GCTCTTC 1 cut(s) 387
BspT107I GGYRCC 2 cut(s) 823, 2347
BspTI CTTAAG 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 2497
BsrDI GCAATG 5 cut(s) 20, 1159, 1346, 1592, 1755
BsrGI TGTACA 1 cut(s) 764
BsrI ACTGG 5 cut(s) 229, 1176, 2102, 2233, 2507
BssECI CCNNGG 5 cut(s) 316, 324, 375, 1212, 2832
BssNAI GTATAC 1 cut(s) 210
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 4 cut(s) 316, 324, 1212, 2832
Bst1107I GTATAC 1 cut(s) 210
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 1892, 3047
Bst4CI ACNGT 7 cut(s) 296, 499, 1549, 1986, 2036, 2240, 2846
Bst6I CTCTTC 6 cut(s) 387, 522, 1292, 1482, 2045, 2139
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 2414
BstAUI TGTACA 1 cut(s) 764
BstBAI YACGTR 1 cut(s) 1913
BstC8I GCNNGC 5 cut(s) 331, 451, 2410, 2704, 2829
BstDSI CCRYGG 1 cut(s) 2832
BstF5I GGATG 4 cut(s) 287, 508, 1208, 1749
BstH2I RGCGCY 2 cut(s) 249, 2600
BstHHI GCGC 2 cut(s) 248, 2599
BstMAI GTCTC 3 cut(s) 2042, 2136, 2780
BstMWI GCNNNNNNNGC 8 cut(s) 134, 965, 1016, 1823, 2117, 2414, 2528, 2722
BstNI CCWGG 2 cut(s) 1892, 3047
BstNSI RCATGY 5 cut(s) 238, 959, 1338, 1609, 2485
BstSCI CCNGG 3 cut(s) 1351, 1890, 3045
BstSFI CTRYAG 1 cut(s) 2200
BstSLI GKGCMC 2 cut(s) 1876, 2101
BstV1I GCAGC 7 cut(s) 250, 812, 920, 971, 1289, 2606, 2965
BstV2I GAAGAC 2 cut(s) 1211, 2171
BstX2I RGATCY 5 cut(s) 47, 427, 1324, 2389, 2454
BstYI RGATCY 5 cut(s) 47, 427, 1324, 2389, 2454
BstZ17I GTATAC 1 cut(s) 210
BsuI GTATCC 2 cut(s) 1127, 2531
BsuRI GGCC 8 cut(s) 728, 1377, 1904, 2099, 2412, 2630, 2831, 3045
BtgI CCRYGG 1 cut(s) 2832
BtsCI GGATG 4 cut(s) 287, 508, 1208, 1749
BtsIMutI CAGTG 6 cut(s) 236, 504, 980, 1183, 1973, 2500
BveI ACCTGC 1 cut(s) 2359
Cac8I GCNNGC 5 cut(s) 331, 451, 2410, 2704, 2829
CfoI GCGC 2 cut(s) 248, 2599
Cfr13I GGNCC 8 cut(s) 162, 321, 726, 1362, 1625, 2097, 2098, 2230
CseI GACGC 3 cut(s) 47, 1934, 2020
CsiI ACCWGGT 1 cut(s) 1890
Csp6I GTAC 4 cut(s) 765, 824, 1910, 2236
CspCI CAANNNNNGTGG 2 cut(s) 1667, 1702
CviQI GTAC 4 cut(s) 765, 824, 1910, 2236
DrdI GACNNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 2 cut(s) 1375, 1902
Eam1104I CTCTTC 6 cut(s) 387, 522, 1292, 1482, 2045, 2139
EarI CTCTTC 6 cut(s) 387, 522, 1292, 1482, 2045, 2139
EciI GGCGGA 1 cut(s) 828
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 4 cut(s) 316, 324, 1212, 2832
Eco147I AGGCCT 1 cut(s) 2412
Eco24I GRGCYC 2 cut(s) 17, 2101
Eco32I GATATC 2 cut(s) 2155, 2428
Eco47I GGWCC 5 cut(s) 162, 321, 1362, 1625, 2230
Eco47III AGCGCT 2 cut(s) 247, 2598
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 851, 1004, 1283, 2120
Eco88I CYCGRG 2 cut(s) 657, 692
EcoICRI GAGCTC 1 cut(s) 15
EcoO109I RGGNCCY 3 cut(s) 1362, 2097, 2098
EcoRI GAATTC 1 cut(s) 570
EcoRII CCWGG 2 cut(s) 1890, 3045
EcoRV GATATC 2 cut(s) 2155, 2428
EcoT14I CCWWGG 4 cut(s) 316, 324, 1212, 2832
EcoT22I ATGCAT 2 cut(s) 1594, 2296
EcoT38I GRGCYC 2 cut(s) 17, 2101
ErhI CCWWGG 4 cut(s) 316, 324, 1212, 2832
FalI AAGNNNNNCTT 2 cut(s) 1196, 1228
FaqI GGGAC 2 cut(s) 334, 2958
FauI CCCGC 1 cut(s) 1248
FauNDI CATATG 2 cut(s) 335, 652
FbaI TGATCA 3 cut(s) 540, 2265, 2962
FblI GTMKAC 2 cut(s) 209, 1887
Fnu4HI GCNGC 8 cut(s) 264, 801, 909, 960, 1303, 2498, 2595, 2954
FokI GGATG 4 cut(s) 274, 515, 1195, 1756
FriOI GRGCYC 2 cut(s) 17, 2101
Fsp4HI GCNGC 8 cut(s) 264, 801, 909, 960, 1303, 2498, 2595, 2954
FspBI CTAG 6 cut(s) 275, 299, 1011, 1277, 2703, 3038
GlaI GCGC 2 cut(s) 247, 2598
GluI GCNGC 8 cut(s) 264, 801, 909, 960, 1303, 2498, 2595, 2954
GsaI CCCAGC 1 cut(s) 1693
GsuI CTGGAG 3 cut(s) 1664, 2593, 2657
HaeII RGCGCY 2 cut(s) 249, 2600
HaeIII GGCC 8 cut(s) 728, 1377, 1904, 2099, 2412, 2630, 2831, 3045
HapII CCGG 1 cut(s) 1353
HgaI GACGC 3 cut(s) 47, 1934, 2020
HhaI GCGC 2 cut(s) 248, 2599
Hin1I GRCGYC 1 cut(s) 39
Hin6I GCGC 2 cut(s) 246, 2597
HinP1I GCGC 2 cut(s) 246, 2597
HincII GTYRAC 2 cut(s) 372, 1131
HindII GTYRAC 2 cut(s) 372, 1131
HindIII AAGCTT 4 cut(s) 89, 1436, 1454, 2243
HpaII CCGG 1 cut(s) 1353
HphI GGTGA 6 cut(s) 887, 910, 1304, 1664, 1759, 2831
Hpy166II GTNNAC 8 cut(s) 210, 372, 765, 1131, 1766, 1874, 1888, 1897
Hpy8I GTNNAC 8 cut(s) 210, 372, 765, 1131, 1766, 1874, 1888, 1897
Hpy99I CGWCG 2 cut(s) 2014, 2783
HpyAV CCTTC 8 cut(s) 716, 979, 1258, 1289, 1375, 2221, 2668, 2917
HpyCH4III ACNGT 7 cut(s) 296, 499, 1549, 1986, 2036, 2240, 2846
HpyCH4IV ACGT 1 cut(s) 1912
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 965, 1016, 1823, 2117, 2414, 2528, 2722
HpySE526I ACGT 1 cut(s) 1912
Hsp92I GRCGYC 1 cut(s) 39
HspAI GCGC 2 cut(s) 246, 2597
KpnI GGTACC 1 cut(s) 827
Ksp22I TGATCA 3 cut(s) 540, 2265, 2962
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 5 cut(s) 12, 647, 1258, 1639, 2638
Lsp1109I GCAGC 7 cut(s) 250, 812, 920, 971, 1289, 2606, 2965
LweI GCATC 2 cut(s) 1802, 2303
MabI ACCWGGT 1 cut(s) 1890
MaeI CTAG 6 cut(s) 275, 299, 1011, 1277, 2703, 3038
MaeII ACGT 1 cut(s) 1912
MaeIII GTNAC 4 cut(s) 607, 754, 856, 2798
MbiI CCGCTC 1 cut(s) 2497
MfeI CAATTG 2 cut(s) 1928, 2369
MflI RGATCY 5 cut(s) 47, 427, 1324, 2389, 2454
MhlI GDGCHC 4 cut(s) 17, 399, 1876, 2101
MlsI TGGCCA 1 cut(s) 1904
MluNI TGGCCA 1 cut(s) 1904
MlyI GAGTC 4 cut(s) 355, 683, 1655, 1745
MmeI TCCRAC 2 cut(s) 315, 1352
Mox20I TGGCCA 1 cut(s) 1904
Mph1103I ATGCAT 2 cut(s) 1594, 2296
MroXI GAANNNNTTC 2 cut(s) 407, 1806
MscI TGGCCA 1 cut(s) 1904
MslI CAYNNNNRTG 3 cut(s) 998, 2289, 2837
Msp20I TGGCCA 1 cut(s) 1904
MspA1I CMGCKG 2 cut(s) 418, 962
MspCI CTTAAG 1 cut(s) 83
MspI CCGG 1 cut(s) 1353
MspR9I CCNGG 3 cut(s) 1353, 1892, 3047
MunI CAATTG 2 cut(s) 1928, 2369
Mva1269I GAATGC 3 cut(s) 1594, 2384, 2596
MvaI CCWGG 2 cut(s) 1892, 3047
MwoI GCNNNNNNNGC 8 cut(s) 134, 965, 1016, 1823, 2117, 2414, 2528, 2722
NciI CCSGG 1 cut(s) 1353
NcoI CCATGG 1 cut(s) 2832
NdeI CATATG 2 cut(s) 335, 652
NheI GCTAGC 1 cut(s) 2702
NlaIV GGNNCC 8 cut(s) 322, 727, 825, 1924, 2098, 2099, 2100, 2349
NmeAIII GCCGAG 2 cut(s) 163, 2324
NmuCI GTSAC 2 cut(s) 856, 2798
NsiI ATGCAT 2 cut(s) 1594, 2296
NspI RCATGY 5 cut(s) 238, 959, 1338, 1609, 2485
PaeR7I CTCGAG 1 cut(s) 692
PaqCI CACCTGC 1 cut(s) 2359
PceI AGGCCT 1 cut(s) 2412
PciI ACATGT 2 cut(s) 1334, 2481
PciSI GCTCTTC 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 1422
PctI GAATGC 3 cut(s) 1594, 2384, 2596
PdmI GAANNNNTTC 2 cut(s) 407, 1806
PkrI GCNGC 8 cut(s) 265, 802, 910, 961, 1304, 2499, 2596, 2955
PleI GAGTC 4 cut(s) 355, 682, 1654, 1744
PpsI GAGTC 4 cut(s) 355, 682, 1654, 1744
Ppu21I YACGTR 1 cut(s) 1913
PpuMI RGGWCCY 1 cut(s) 1362
PscI ACATGT 2 cut(s) 1334, 2481
PshBI ATTAAT 3 cut(s) 750, 834, 1778
Psp124BI GAGCTC 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 1362
Psp6I CCWGG 2 cut(s) 1890, 3045
PspFI CCCAGC 1 cut(s) 1689
PspGI CCWGG 2 cut(s) 1890, 3045
PspN4I GGNNCC 8 cut(s) 322, 727, 825, 1924, 2098, 2099, 2100, 2349
PspOMI GGGCCC 1 cut(s) 2097
PspPI GGNCC 8 cut(s) 162, 321, 726, 1362, 1625, 2097, 2098, 2230
PspPPI RGGWCCY 1 cut(s) 1362
PsuI RGATCY 5 cut(s) 47, 427, 1324, 2389, 2454
PvuII CAGCTG 2 cut(s) 418, 962
RsaI GTAC 4 cut(s) 766, 825, 1911, 2237
RsaNI GTAC 4 cut(s) 765, 824, 1910, 2236
RseI CAYNNNNRTG 3 cut(s) 998, 2289, 2837
SacI GAGCTC 1 cut(s) 17
SapI GCTCTTC 1 cut(s) 387
SatI GCNGC 8 cut(s) 264, 801, 909, 960, 1303, 2498, 2595, 2954
Sau96I GGNCC 8 cut(s) 162, 321, 726, 1362, 1625, 2097, 2098, 2230
SchI GAGTC 4 cut(s) 355, 683, 1655, 1745
ScrFI CCNGG 3 cut(s) 1353, 1892, 3047
SduI GDGCHC 4 cut(s) 17, 399, 1876, 2101
SexAI ACCWGGT 1 cut(s) 1890
SfaNI GCATC 2 cut(s) 1802, 2303
SfcI CTRYAG 1 cut(s) 2200
Sfr274I CTCGAG 1 cut(s) 692
SinI GGWCC 5 cut(s) 162, 321, 1362, 1625, 2230
SlaI CTCGAG 1 cut(s) 692
SmiMI CAYNNNNRTG 3 cut(s) 998, 2289, 2837
SmlI CTYRAG 5 cut(s) 83, 384, 692, 1658, 2669
SmoI CTYRAG 5 cut(s) 83, 384, 692, 1658, 2669
SpeI ACTAGT 1 cut(s) 3037
SseBI AGGCCT 1 cut(s) 2412
SsiI CCGC 4 cut(s) 813, 1255, 2497, 2792
SspMI CTAG 6 cut(s) 275, 299, 1011, 1277, 2703, 3038
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 2412
StyD4I CCNGG 3 cut(s) 1351, 1890, 3045
StyI CCWWGG 4 cut(s) 316, 324, 1212, 2832
TaaI ACNGT 7 cut(s) 296, 499, 1549, 1986, 2036, 2240, 2846
TaiI ACGT 1 cut(s) 1915
TaqI TCGA 3 cut(s) 191, 693, 1416
TatI WGTACW 1 cut(s) 764
TauI GCSGC 1 cut(s) 2500
TscAI CASTG 6 cut(s) 236, 504, 987, 1183, 1980, 2507
TseFI GTSAC 2 cut(s) 856, 2798
TseI GCWGC 7 cut(s) 263, 800, 908, 959, 1302, 2594, 2953
Tsp45I GTSAC 2 cut(s) 856, 2798
TspGWI ACGGA 1 cut(s) 1795
TspRI CASTG 6 cut(s) 236, 504, 987, 1183, 1980, 2507
Vha464I CTTAAG 1 cut(s) 83
VneI GTGCAC 1 cut(s) 1872
VpaK11BI GGWCC 5 cut(s) 162, 321, 1362, 1625, 2230
VspI ATTAAT 3 cut(s) 750, 834, 1778
XapI RAATTY 7 cut(s) 570, 1309, 1663, 1978, 2050, 2873, 3027
XbaI TCTAGA 1 cut(s) 1276
XceI RCATGY 5 cut(s) 238, 959, 1338, 1609, 2485
XcmI CCANNNNNNNNNTGG 1 cut(s) 1736
XhoI CTCGAG 1 cut(s) 692
XmiI GTMKAC 2 cut(s) 209, 1887
XmnI GAANNNNTTC 2 cut(s) 407, 1806
XspI CTAG 6 cut(s) 275, 299, 1011, 1277, 2703, 3038
Zsp2I ATGCAT 2 cut(s) 1594, 2296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.