Rh4AG074000

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006689
Physical Location & Seq
Forward (+)
15187752 .. 15191893
4142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG074000.1

Sequence Viewer

Length: 861 bp
ATGGAGGGAGGGAGCTCAGAATTGCAGAGCATACAAGACGCCATCAGATCCTCAGATGTGGTGAATACTTGGATTGAATTACTTAAGAAGCTTGGCGATTTGAAAATAACAGAGAAGTCTGAATTGGCTTCCCTTGCCGAGTGTTTGACAGAGTATACTTGCTTGGATTTAAGCCAGTGCATGTTAAATAGCGCTATATTACAGGTGGCTGCGAAGCACCTAGAATCAGACACATCCAACTGTCTAGCACATTTCCTTGTCCTTGGGACCAAGGCAAGCATATGGTGTGGAAAGCACCTAAAAATGACTCTTATGTCAACCGAGGAATCTCAAGAAGAAGAGCACGAGAACCTGTTCTTTCAGCTGTTTCTGGATCTGCTGAGCTTTTTGAGGGCTTGCTTTTCAGCTTTGGCAAGATACCCTGTTTCTGTTGAGAAGATGACAATAAAGAGAATTCAGTCATTTGTATCAGAAGAAGTCGTAGTAAAGGTTACACAGGAGGTCATTGATTCTGTAATAAGATTGTGTGGAGCATATGCCCGAGCTTTGAATTGGGAGTCTTGGGAAGAAAAACTCGAGAGGGATAAAACTGACATGGACTTTGAAGGGGCCAATACTAATAATGGCGGAAGTCTGGTACCGATTATTAATATGTCGTGGAAAGGTGTGGTCACTTTGCTTCAGCTCGGTGATGGGGTATTAGCAACAAAGGTGAATGTAGCAGCTCTAATATCAAATCTAATCTCATTGGTTAATGAATCTATAAGACATGCAGCTGAGGCATGGTTTTCATCACTGAAGGAAAACATCTCTGTGTCAGAAGCTAGAAAGAACTCTTGGAGAAGACATCCTTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

286

Amino Acids

32.03

Weight (kDa)

5.2

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 215 - 276 1.6e-13 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 313
Acc65I GGTACC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 637
AccI GTMKAC 1 cut(s) 155
AciI CCGC 1 cut(s) 627
AclWI GGATC 2 cut(s) 42, 381
AcsI RAATTY 1 cut(s) 453
AcuI CTGAAG 2 cut(s) 665, 818
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 639
AfeI AGCGCT 1 cut(s) 193
AflII CTTAAG 1 cut(s) 83
AgsI TTSAA 4 cut(s) 77, 103, 550, 605
AjuI GAANNNNNNNTTGG 5 cut(s) 107, 139, 820, 835, 852
Alw21I GWGCWC 2 cut(s) 17, 345
AlwI GGATC 2 cut(s) 42, 381
Ama87I CYCGRG 2 cut(s) 540, 575
Aor51HI AGCGCT 1 cut(s) 193
AoxI GGCC 1 cut(s) 609
ApeKI GCWGC 3 cut(s) 209, 722, 773
ApoI RAATTY 1 cut(s) 453
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AseI ATTAAT 1 cut(s) 648
Asp700I GAANNNNTTC 1 cut(s) 353
Asp718I GGTACC 1 cut(s) 637
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 267, 609
AsuHPI GGTGA 3 cut(s) 73, 701, 724
AvaI CYCGRG 2 cut(s) 540, 575
AvaII GGWCC 1 cut(s) 267
BanI GGYRCC 1 cut(s) 637
BanII GRGCYC 1 cut(s) 17
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 850
Bbv12I GWGCWC 2 cut(s) 17, 345
BbvCI CCTCAGC 1 cut(s) 777
BbvI GCAGC 3 cut(s) 196, 734, 785
BccI CCATC 2 cut(s) 50, 686
BfaI CTAG 3 cut(s) 221, 245, 825
BfoI RGCGCY 1 cut(s) 195
BfrI CTTAAG 1 cut(s) 83
BisI GCNGC 3 cut(s) 210, 723, 774
BlpI GCTNAGC 1 cut(s) 380
BlsI GCNGC 3 cut(s) 211, 724, 775
Bme18I GGWCC 1 cut(s) 267
BmeT110I CYCGRG 2 cut(s) 540, 575
BmgT120I GGNCC 2 cut(s) 267, 609
BmiI GGNNCC 3 cut(s) 268, 610, 639
BpiI GAAGAC 1 cut(s) 850
Bpu10I CCTNAGC 1 cut(s) 777
Bpu1102I GCTNAGC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 262, 270, 321, 851
Bse1I ACTGG 1 cut(s) 175
BseDI CCNNGG 4 cut(s) 262, 270, 321, 851
BseGI GGATG 2 cut(s) 233, 847
BseMII CTCAG 4 cut(s) 30, 66, 371, 768
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 3 cut(s) 196, 734, 785
BshFI GGCC 1 cut(s) 611
BshNI GGYRCC 1 cut(s) 637
BsiHKAI GWGCWC 2 cut(s) 17, 345
BsiHKCI CYCGRG 2 cut(s) 540, 575
BslFI GGGAC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 1 cut(s) 611
BsoBI CYCGRG 2 cut(s) 540, 575
Bsp1286I GDGCHC 2 cut(s) 17, 345
Bsp143I GATC 2 cut(s) 47, 373
Bsp1720I GCTNAGC 1 cut(s) 380
BspACI CCGC 1 cut(s) 627
BspANI GGCC 1 cut(s) 611
BspCNI CTCAG 4 cut(s) 29, 65, 372, 769
BspLI GGNNCC 3 cut(s) 268, 610, 639
BspPI GGATC 2 cut(s) 42, 381
BspQI GCTCTTC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 637
BspTI CTTAAG 1 cut(s) 83
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 4 cut(s) 262, 270, 321, 851
BssMI GATC 2 cut(s) 47, 373
BssNAI GTATAC 1 cut(s) 156
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 3 cut(s) 262, 270, 851
Bst1107I GTATAC 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 333
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 277, 397
BstDEI CTNAG 4 cut(s) 16, 52, 380, 777
BstF5I GGATG 2 cut(s) 233, 847
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 2 cut(s) 50, 376
BstMBI GATC 2 cut(s) 47, 373
BstMWI GCNNNNNNNGC 2 cut(s) 134, 779
BstNSI RCATGY 2 cut(s) 184, 773
BstV1I GCAGC 3 cut(s) 196, 734, 785
BstV2I GAAGAC 1 cut(s) 850
BstX2I RGATCY 2 cut(s) 47, 373
BstYI RGATCY 2 cut(s) 47, 373
BstZ17I GTATAC 1 cut(s) 156
BsuRI GGCC 1 cut(s) 611
BtsCI GGATG 2 cut(s) 233, 847
BtsIMutI CAGTG 2 cut(s) 182, 794
Cac8I GCNNGC 2 cut(s) 277, 397
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 267, 609
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 638
CviAII CATG 4 cut(s) 181, 595, 770, 783
CviQI GTAC 1 cut(s) 638
DdeI CTNAG 4 cut(s) 16, 52, 380, 777
DpnI GATC 2 cut(s) 49, 375
DpnII GATC 2 cut(s) 47, 373
DrdI GACNNNNNNGTC 1 cut(s) 313
DseDI GACNNNNNNGTC 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 1 cut(s) 642
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 3 cut(s) 262, 270, 851
Eco24I GRGCYC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 267
Eco47III AGCGCT 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 2 cut(s) 665, 818
Eco88I CYCGRG 2 cut(s) 540, 575
EcoICRI GAGCTC 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 453
EcoT14I CCWWGG 3 cut(s) 262, 270, 851
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 3 cut(s) 262, 270, 851
FaeI CATG 4 cut(s) 184, 598, 773, 786
FalI AAGNNNNNCTT 1 cut(s) 835
FaqI GGGAC 1 cut(s) 280
FatI CATG 4 cut(s) 180, 594, 769, 782
FauNDI CATATG 2 cut(s) 281, 535
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 3 cut(s) 210, 723, 774
FokI GGATG 2 cut(s) 220, 834
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 3 cut(s) 210, 723, 774
FspBI CTAG 3 cut(s) 221, 245, 825
GlaI GCGC 1 cut(s) 193
GluI GCNGC 3 cut(s) 210, 723, 774
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 1 cut(s) 611
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 184, 598, 773, 786
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 6 cut(s) 224, 307, 326, 509, 557, 758
HphI GGTGA 3 cut(s) 73, 701, 724
Hpy166II GTNNAC 2 cut(s) 156, 318
Hpy188I TCNGA 7 cut(s) 19, 47, 55, 121, 229, 472, 820
Hpy188III TCNNGA 3 cut(s) 332, 371, 577
Hpy8I GTNNAC 2 cut(s) 156, 318
HpyAV CCTTC 2 cut(s) 599, 793
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 3 cut(s) 25, 180, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 779
HpyF3I CTNAG 4 cut(s) 16, 52, 380, 777
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 184, 598, 773, 786
HspAI GCGC 1 cut(s) 192
KpnI GGTACC 1 cut(s) 641
Kzo9I GATC 2 cut(s) 47, 373
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 2 cut(s) 12, 530
LpnPI CCDG 7 cut(s) 188, 188, 356, 365, 435, 482, 620
Lsp1109I GCAGC 3 cut(s) 196, 734, 785
MaeI CTAG 3 cut(s) 221, 245, 825
MaeIII GTNAC 2 cut(s) 490, 670
MalI GATC 2 cut(s) 49, 375
MboI GATC 2 cut(s) 47, 373
MboII GAAGA 6 cut(s) 347, 350, 448, 485, 578, 855
MflI RGATCY 2 cut(s) 47, 373
MhlI GDGCHC 2 cut(s) 17, 345
MluCI AATT 5 cut(s) 20, 77, 122, 453, 550
MlyI GAGTC 2 cut(s) 301, 566
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 6 cut(s) 61, 316, 384, 493, 573, 772
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 5 cut(s) 84, 170, 185, 648, 753
MslI CAYNNNNRTG 1 cut(s) 812
MspA1I CMGCKG 2 cut(s) 364, 776
MspCI CTTAAG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 134, 779
NdeI CATATG 2 cut(s) 281, 535
NdeII GATC 2 cut(s) 47, 373
NlaIII CATG 4 cut(s) 184, 598, 773, 786
NlaIV GGNNCC 3 cut(s) 268, 610, 639
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 670
NspI RCATGY 2 cut(s) 184, 773
PaeR7I CTCGAG 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 4 cut(s) 224, 326, 509, 758
PkrI GCNGC 3 cut(s) 211, 724, 775
PleI GAGTC 2 cut(s) 301, 565
PpsI GAGTC 2 cut(s) 301, 565
PshBI ATTAAT 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 17
PspN4I GGNNCC 3 cut(s) 268, 610, 639
PspPI GGNCC 2 cut(s) 267, 609
PsuI RGATCY 2 cut(s) 47, 373
PvuII CAGCTG 2 cut(s) 364, 776
RsaI GTAC 1 cut(s) 639
RsaNI GTAC 1 cut(s) 638
RseI CAYNNNNRTG 1 cut(s) 812
SacI GAGCTC 1 cut(s) 17
SapI GCTCTTC 1 cut(s) 333
SaqAI TTAA 5 cut(s) 84, 170, 185, 648, 753
SatI GCNGC 3 cut(s) 210, 723, 774
Sau3AI GATC 2 cut(s) 47, 373
Sau96I GGNCC 2 cut(s) 267, 609
SchI GAGTC 2 cut(s) 301, 566
SduI GDGCHC 2 cut(s) 17, 345
Sfr274I CTCGAG 1 cut(s) 575
SinI GGWCC 1 cut(s) 267
SlaI CTCGAG 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 3 cut(s) 83, 330, 575
SmoI CTYRAG 3 cut(s) 83, 330, 575
Sse9I AATT 5 cut(s) 20, 77, 122, 453, 550
SsiI CCGC 1 cut(s) 627
SspMI CTAG 3 cut(s) 221, 245, 825
SstI GAGCTC 1 cut(s) 17
StyI CCWWGG 3 cut(s) 262, 270, 851
TaaI ACNGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 576
TasI AATT 5 cut(s) 20, 77, 122, 453, 550
TfiI GAWTC 4 cut(s) 224, 326, 509, 758
Tru1I TTAA 5 cut(s) 84, 170, 185, 648, 753
Tru9I TTAA 5 cut(s) 84, 170, 185, 648, 753
TscAI CASTG 2 cut(s) 182, 801
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 3 cut(s) 209, 722, 773
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 2 cut(s) 771, 780
TspRI CASTG 2 cut(s) 182, 801
Vha464I CTTAAG 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 267
VspI ATTAAT 1 cut(s) 648
XapI RAATTY 1 cut(s) 453
XceI RCATGY 2 cut(s) 184, 773
XhoI CTCGAG 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 3 cut(s) 221, 245, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.