Rh4DG068400

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006689
Physical Location & Seq
Reverse (-)
10177444 .. 10183903
6460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG068400.1

Sequence Viewer

Length: 3009 bp
ATGGAGGGAGGGAGCTCAGAATTGCAGAGCATACAAGAAGCCATCAGATCCTCAGATGTGGTGGAGAATCGGATTGAATTACTTAAGAAGCTTGGCGATTTGAAAATAACAGAGAAGTCTGAATTGGCTTCCCTTGCCGAGTGTTTGACAGAGTATACTTGCTTGGATTTAAGCCAGTGCATGTTAAATAGCGCTATATTACAGGTGGCTGCAAAGCACCTAGAATCAGACACATCCAACTGTCTAGCACATTTCCTTGTCCTTGGGACCAAGGCAAGCATATGGTGTGGAAAGCACCTAAAAATGACTCTTATGTCAACCGAGGAATCTCAAGAAGAAGAGCACGGGAACCTGTTCTTTCAGCTGTTTCTGGATCTGCTGAGCTTTTTGAGGGCTTGCTTTTCAGCTTTGGCAAGATACCCTGTTTCTGTTGAGAAGATGACAGTGGATATAGTTGAAACATTCTTTGATGAAGAGTTAAACTTGATCAAATATTCTATATCAGAGATAAAGAGAATTCAGTCATTTGTATCAGAAGAAGTAGTAGTAAAGGTCACACAGGAGGTCATTGATTCTGTAATAAGACTGTGTGGAGCATATGCCCGAGCTTTGAATTGGGATTCTTGGGAAGAAAAACTCGAGAGGGATAAAACTGACATGGACTTTGAAGGGGCCAATGGTATGAATCATGTTATTAATGTAACAAAGTGTACAATAGAAAAACTATGTGAGATGGGCATTATTGCAGCTAATAATGGCGGAAGTCTGGTACCGATTATTAATATGTCGTGGAAAGGTGTGGTCACTTTGCTTCAGCTCGGTGATGGGGTATTAGCAACAAAGGTGAATGTAGCAGCTTTAATTTCAAATCTAATCTCATTGGTTAATGAATCTATAAGACATGCATCTGAGGCATGGTTTTCATCACTGAAGGAAAACATCTCTGTGTCAGAAGCTAGAAAGGCATTTCTTCCTGTAAAGTTTTATTTGGTTAATGCAGTAAAAATATCTTCCCTATACCCATATCAAGCATATCTGGTGCAAAGTGAGATAACAAAATGTATCCTGATGATGTCAACCTTAAAGATTTCATTGAGCAATGAAAAACTGTTAAAAACTGCCAGTGATGTTTTCACAGAACTCTTGGAGAAGACATCCTTGGATTTGCTTCTTGCTCTACTGAACTCAGTTCAAGTGAAGCGGGAGCTCAAGGTTGAGATTCTAGACTATCTCTTCAGTGAAGGAAGCTGCACAAATTCTGTTGCTGGAGATCCGACTACATGTGGGGAAGCATTGCCCGGAGAAAGAACCTTCTTGATTGGCCGTGTTTCATTGTTTCTTAATTTTCTGCGATTTTCTCTCGATCTTGACGATGATGTAAAGCTTGGGATTGCTGGAAAGCTTGGTTGGTTCTTTGATATGTTAGTAAATGAAGAGGTATATTCTTCCATTCTTCTTTTGCAAGTTCCTGTGCTATATGGTTCTGGAAAAACTGTGGAAGTTGTCTGGCAGCCTTTGTTTTCTTCTATTTTGAATGCATTGCAAATCTACATGCTTGTGGTTTCTTCTGGTCCTGCTTGGAGGGAGTTGGAGTCTTTTCTTCTTGAGAATTTATTTCACCCCCACTTTCTTTGCTGGGAGATTGTAATGGAACTTTGGTGCTTTATGGTTCGCCATGCTGAGTCAGGGATGGTGAGTGGCATTGCTGGTAAACTTTGCTCTTTAATGAAGTTGGTGGCATCTACTGAATCAGTTCTTGTAGCTGATTCTGCTCTGAGAAAAGTGGCAAGGTCAATCAGCATAATTCTTACTGTTGGTGCACAATCTATGGTAGACCAGGTTTACATGGCCATTGTACGTGATGAGGGAACCCAATTGTCATCAGTTATGCGTCTGGCGTTGTTCATGGAGGGCTTCCCACTGAATTTACTGTCAGACAAGATGAAAAGAATTGCGACGCAAAGGATAATTACTGATTACTGTCTCTTCATTGAAAATTTTGATGAGAAGTCAATGAAATCTCTTGATTGTGGTCTTTTTGGGGCCCCAGTTTTTGCTTTGTCAGCTTCGTTGCAGTCTCTTCAGATCAGCACATCTGATATCAATGTGAAGACCTTGAAGTTTTTAGTTGCCATCATTCATAACTACAGGGTTTGTGAAGATCAGCTAATGAAGGACCAGTACCGTAAGCTTCTGAGTGAAACATTAGTGATCATCTCAAATATGAAGCACCTATATGCATCTCTTGAAATGGAGAAAGTAATCTGTGAGCTTGAAAACCTGTTTATCTCGGGACCTGCTGTTTCTGATACCCAATTGTATGAATGCAAAACAGATCTGGCTCTTTTCATGGCAGGCCTTGCTCACATGGATATCAAAGAAGCTGATGAAAGTTCCAAGATCTCTGCTCTATGGGATTTGTATCACATGTTATTGGGAGAGCGGCACTGGGCATTTATTCATTTGGCAATTACAGCATTTGGATACTTTTCTGCTCGGACAAATTGCAATAAGCTCTGGAGATTCGTGCCTCAGAATGCAGCGCTTTCCTATGATCTGACTTCTGGAAATGAGGCAAATCTGGAGCGATTTATGTCTGAGTTCAAGGTTTTTCTTGAGAAGGAAACGGCACTTCTCACAATAACGGCTAGCTCTGACCAGCTTGGTCTGCTTGTGAGGGAGGGTCTAAAACTAAAAGAAATGTTCCAAAAGACTTCAAATGTCGTCGTTGAGACTGCGGAATGTGACTACATGGAAATTGATGGTGAAAAGCAGGCCATGGAGATGAACAGTGGAAAACAATCCAATAGGAAAAGGAAATTTCCTGATGGAATTGAAAAGGGAATGGAACTGCTACAAAGTGGTATGAAGGTTATTGTGGATAGTATTTCTCAGTTGCAGCAAAATGATCAACCCAGCTCCAATGAACTACCCGACAACTTCTTGACCACCTTTTCTCGCCTTGAAGCTGAAATTTCTCAACTAGTTGGCCTGGCTGGTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

1002

Amino Acids

112.1

Weight (kDa)

4.99

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 259 - 839 1.4e-148 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 313
Acc36I ACCTGC 1 cut(s) 2305
Acc65I GGTACC 1 cut(s) 769
AccB1I GGYRCC 1 cut(s) 769
AccBSI CCGCTC 1 cut(s) 2443
AccI GTMKAC 2 cut(s) 155, 1833
AciI CCGC 4 cut(s) 759, 1201, 2443, 2738
AclWI GGATC 3 cut(s) 42, 381, 1265
AcoI YGGCCR 2 cut(s) 1321, 1848
AcsI RAATTY 7 cut(s) 516, 1255, 1609, 1924, 1996, 2819, 2973
AcuI CTGAAG 4 cut(s) 797, 950, 1219, 2066
AfaI GTAC 4 cut(s) 712, 771, 1857, 2183
AfeI AGCGCT 2 cut(s) 193, 2544
AflII CTTAAG 1 cut(s) 83
AflIII ACRYGT 2 cut(s) 1280, 2427
AhlI ACTAGT 1 cut(s) 2983
AjnI CCWGG 2 cut(s) 1836, 2991
AjuI GAANNNNNNNTTGG 6 cut(s) 107, 139, 1142, 1174, 1390, 1422
Alw21I GWGCWC 4 cut(s) 17, 345, 1209, 1822
Alw26I GTCTC 3 cut(s) 1988, 2082, 2726
Alw44I GTGCAC 1 cut(s) 1818
AlwI GGATC 3 cut(s) 42, 381, 1265
Ama87I CYCGRG 3 cut(s) 603, 638, 2290
Aor51HI AGCGCT 2 cut(s) 193, 2544
AoxI GGCC 7 cut(s) 672, 1321, 1848, 2043, 2356, 2775, 2989
ApaI GGGCCC 1 cut(s) 2047
ApaLI GTGCAC 1 cut(s) 1818
ApeKI GCWGC 7 cut(s) 209, 746, 854, 1248, 1510, 2540, 2899
ApoI RAATTY 7 cut(s) 516, 1255, 1609, 1924, 1996, 2819, 2973
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AseI ATTAAT 2 cut(s) 696, 780
Asp700I GAANNNNTTC 2 cut(s) 353, 1752
Asp718I GGTACC 1 cut(s) 769
AspLEI GCGC 2 cut(s) 194, 2545
AspS9I GGNCC 7 cut(s) 267, 672, 1571, 2043, 2044, 2176, 2294
AsuC2I CCSGG 1 cut(s) 1299
AsuHPI GGTGA 5 cut(s) 833, 856, 1610, 1705, 2777
AsuNHI GCTAGC 1 cut(s) 2648
AvaI CYCGRG 3 cut(s) 603, 638, 2290
AvaII GGWCC 4 cut(s) 267, 1571, 2176, 2294
BaeGI GKGCMC 2 cut(s) 1822, 2047
BalI TGGCCA 1 cut(s) 1850
BanI GGYRCC 1 cut(s) 769
BanII GRGCYC 3 cut(s) 17, 1209, 2047
BarI GAAGNNNNNNTAC 2 cut(s) 2165, 2197
BbsI GAAGAC 2 cut(s) 1157, 2117
Bbv12I GWGCWC 4 cut(s) 17, 345, 1209, 1822
BbvI GCAGC 7 cut(s) 196, 758, 866, 1235, 1522, 2552, 2911
BccI CCATC 7 cut(s) 50, 727, 818, 1684, 2141, 2756, 2822
BceAI ACGGC 3 cut(s) 1308, 2643, 2661
BciT130I CCWGG 2 cut(s) 1838, 2993
BciVI GTATCC 2 cut(s) 1073, 2477
BclI TGATCA 3 cut(s) 486, 2211, 2908
BcnI CCSGG 1 cut(s) 1299
BcoDI GTCTC 3 cut(s) 1988, 2082, 2726
BcuI ACTAGT 1 cut(s) 2983
BfaI CTAG 6 cut(s) 221, 245, 957, 1223, 2649, 2984
BfmI CTRYAG 1 cut(s) 2146
BfoI RGCGCY 2 cut(s) 195, 2546
BfrI CTTAAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 2305
BfuI GTATCC 2 cut(s) 1073, 2477
BglII AGATCT 2 cut(s) 2335, 2400
BisI GCNGC 8 cut(s) 210, 747, 855, 1249, 1511, 2444, 2541, 2900
BlpI GCTNAGC 1 cut(s) 380
BlsI GCNGC 8 cut(s) 211, 748, 856, 1250, 1512, 2445, 2542, 2901
Bme1390I CCNGG 3 cut(s) 1299, 1838, 2993
Bme18I GGWCC 4 cut(s) 267, 1571, 2176, 2294
BmeT110I CYCGRG 3 cut(s) 603, 638, 2290
BmgT120I GGNCC 7 cut(s) 267, 672, 1571, 2043, 2044, 2176, 2294
BmiI GGNNCC 9 cut(s) 268, 350, 673, 771, 1870, 2044, 2045, 2046, 2295
BmrFI CCNGG 3 cut(s) 1299, 1838, 2993
BmrI ACTGGG 2 cut(s) 2042, 2458
BmsI GCATC 3 cut(s) 914, 1748, 2249
BmtI GCTAGC 1 cut(s) 2652
BmuI ACTGGG 2 cut(s) 2042, 2458
BpiI GAAGAC 2 cut(s) 1157, 2117
BpmI CTGGAG 3 cut(s) 1287, 2539, 2603
Bpu1102I GCTNAGC 1 cut(s) 380
BpuEI CTTGAG 4 cut(s) 315, 1193, 1625, 2636
BpuMI CCSGG 1 cut(s) 1299
BsaAI YACGTR 1 cut(s) 1859
BsaBI GATNNNNATC 1 cut(s) 2421
BsaJI CCNNGG 5 cut(s) 262, 270, 321, 1158, 2778
BsaXI ACNNNNNCTCC 2 cut(s) 1577, 1607
Bse1I ACTGG 5 cut(s) 175, 1122, 2048, 2179, 2453
Bse3DI GCAATG 4 cut(s) 1105, 1292, 1538, 1701
Bse8I GATNNNNATC 1 cut(s) 2421
BseBI CCWGG 2 cut(s) 1838, 2993
BseDI CCNNGG 5 cut(s) 262, 270, 321, 1158, 2778
BseGI GGATG 3 cut(s) 233, 1154, 1695
BseJI GATNNNNATC 1 cut(s) 2421
BseMI GCAATG 4 cut(s) 1105, 1292, 1538, 1701
BseNI ACTGG 5 cut(s) 175, 1122, 2048, 2179, 2453
BseSI GKGCMC 2 cut(s) 1822, 2047
BseXI GCAGC 7 cut(s) 196, 758, 866, 1235, 1522, 2552, 2911
BseYI CCCAGC 2 cut(s) 1635, 2915
BsgI GTGCAG 1 cut(s) 1234
BshFI GGCC 7 cut(s) 674, 1323, 1850, 2045, 2358, 2777, 2991
BshNI GGYRCC 1 cut(s) 769
BsiHKAI GWGCWC 4 cut(s) 17, 345, 1209, 1822
BsiHKCI CYCGRG 3 cut(s) 603, 638, 2290
BsiSI CCGG 1 cut(s) 1299
BslFI GGGAC 2 cut(s) 280, 2307
BsmAI GTCTC 3 cut(s) 1988, 2082, 2726
BsmFI GGGAC 2 cut(s) 280, 2307
BsmI GAATGC 3 cut(s) 1540, 2330, 2542
BsnI GGCC 7 cut(s) 674, 1323, 1850, 2045, 2358, 2777, 2991
BsoBI CYCGRG 3 cut(s) 603, 638, 2290
Bsp120I GGGCCC 1 cut(s) 2043
Bsp1286I GDGCHC 5 cut(s) 17, 345, 1209, 1822, 2047
Bsp1407I TGTACA 1 cut(s) 710
Bsp1720I GCTNAGC 1 cut(s) 380
Bsp19I CCATGG 1 cut(s) 2778
BspACI CCGC 4 cut(s) 759, 1201, 2443, 2738
BspANI GGCC 7 cut(s) 674, 1323, 1850, 2045, 2358, 2777, 2991
BspLI GGNNCC 9 cut(s) 268, 350, 673, 771, 1870, 2044, 2045, 2046, 2295
BspMI ACCTGC 1 cut(s) 2305
BspOI GCTAGC 1 cut(s) 2652
BspPI GGATC 3 cut(s) 42, 381, 1265
BspQI GCTCTTC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 769
BspTI CTTAAG 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 2443
BsrDI GCAATG 4 cut(s) 1105, 1292, 1538, 1701
BsrGI TGTACA 1 cut(s) 710
BsrI ACTGG 5 cut(s) 175, 1122, 2048, 2179, 2453
BssECI CCNNGG 5 cut(s) 262, 270, 321, 1158, 2778
BssNAI GTATAC 1 cut(s) 156
BssT1I CCWWGG 4 cut(s) 262, 270, 1158, 2778
Bst1107I GTATAC 1 cut(s) 156
Bst2UI CCWGG 2 cut(s) 1838, 2993
Bst6I CTCTTC 6 cut(s) 333, 468, 1238, 1428, 1991, 2085
BstAFI CTTAAG 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 2360
BstAUI TGTACA 1 cut(s) 710
BstBAI YACGTR 1 cut(s) 1859
BstC8I GCNNGC 5 cut(s) 277, 397, 2356, 2650, 2775
BstDSI CCRYGG 1 cut(s) 2778
BstF5I GGATG 3 cut(s) 233, 1154, 1695
BstH2I RGCGCY 2 cut(s) 195, 2546
BstHHI GCGC 2 cut(s) 194, 2545
BstMAI GTCTC 3 cut(s) 1988, 2082, 2726
BstMWI GCNNNNNNNGC 8 cut(s) 134, 911, 962, 1769, 2063, 2360, 2474, 2668
BstNI CCWGG 2 cut(s) 1838, 2993
BstNSI RCATGY 5 cut(s) 184, 905, 1284, 1555, 2431
BstSCI CCNGG 3 cut(s) 1297, 1836, 2991
BstSFI CTRYAG 1 cut(s) 2146
BstSLI GKGCMC 2 cut(s) 1822, 2047
BstV1I GCAGC 7 cut(s) 196, 758, 866, 1235, 1522, 2552, 2911
BstV2I GAAGAC 2 cut(s) 1157, 2117
BstX2I RGATCY 5 cut(s) 47, 373, 1270, 2335, 2400
BstYI RGATCY 5 cut(s) 47, 373, 1270, 2335, 2400
BstZ17I GTATAC 1 cut(s) 156
BsuI GTATCC 2 cut(s) 1073, 2477
BsuRI GGCC 7 cut(s) 674, 1323, 1850, 2045, 2358, 2777, 2991
BtgI CCRYGG 1 cut(s) 2778
BtsCI GGATG 3 cut(s) 233, 1154, 1695
BtsIMutI CAGTG 8 cut(s) 182, 450, 926, 1129, 1243, 1919, 2446, 2797
BveI ACCTGC 1 cut(s) 2305
Cac8I GCNNGC 5 cut(s) 277, 397, 2356, 2650, 2775
CfoI GCGC 2 cut(s) 194, 2545
Cfr13I GGNCC 7 cut(s) 267, 672, 1571, 2043, 2044, 2176, 2294
CseI GACGC 2 cut(s) 1880, 1966
CsiI ACCWGGT 1 cut(s) 1836
Csp6I GTAC 4 cut(s) 711, 770, 1856, 2182
CspCI CAANNNNNGTGG 2 cut(s) 1613, 1648
CviQI GTAC 4 cut(s) 711, 770, 1856, 2182
DrdI GACNNNNNNGTC 1 cut(s) 313
DseDI GACNNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 2 cut(s) 1321, 1848
Eam1104I CTCTTC 6 cut(s) 333, 468, 1238, 1428, 1991, 2085
EarI CTCTTC 6 cut(s) 333, 468, 1238, 1428, 1991, 2085
EciI GGCGGA 1 cut(s) 774
Ecl136II GAGCTC 2 cut(s) 15, 1207
Eco130I CCWWGG 4 cut(s) 262, 270, 1158, 2778
Eco147I AGGCCT 1 cut(s) 2358
Eco24I GRGCYC 3 cut(s) 17, 1209, 2047
Eco32I GATATC 2 cut(s) 2101, 2374
Eco47I GGWCC 4 cut(s) 267, 1571, 2176, 2294
Eco47III AGCGCT 2 cut(s) 193, 2544
Eco53kI GAGCTC 2 cut(s) 15, 1207
Eco57I CTGAAG 4 cut(s) 797, 950, 1219, 2066
Eco88I CYCGRG 3 cut(s) 603, 638, 2290
EcoICRI GAGCTC 2 cut(s) 15, 1207
EcoO109I RGGNCCY 3 cut(s) 2043, 2044, 2294
EcoRI GAATTC 1 cut(s) 516
EcoRII CCWGG 2 cut(s) 1836, 2991
EcoRV GATATC 2 cut(s) 2101, 2374
EcoT14I CCWWGG 4 cut(s) 262, 270, 1158, 2778
EcoT22I ATGCAT 3 cut(s) 907, 1540, 2242
EcoT38I GRGCYC 3 cut(s) 17, 1209, 2047
ErhI CCWWGG 4 cut(s) 262, 270, 1158, 2778
FalI AAGNNNNNCTT 2 cut(s) 1142, 1174
FaqI GGGAC 2 cut(s) 280, 2307
FauI CCCGC 1 cut(s) 1194
FauNDI CATATG 2 cut(s) 281, 598
FbaI TGATCA 3 cut(s) 486, 2211, 2908
FblI GTMKAC 2 cut(s) 155, 1833
Fnu4HI GCNGC 8 cut(s) 210, 747, 855, 1249, 1511, 2444, 2541, 2900
FokI GGATG 3 cut(s) 220, 1141, 1702
FriOI GRGCYC 3 cut(s) 17, 1209, 2047
Fsp4HI GCNGC 8 cut(s) 210, 747, 855, 1249, 1511, 2444, 2541, 2900
FspBI CTAG 6 cut(s) 221, 245, 957, 1223, 2649, 2984
GlaI GCGC 2 cut(s) 193, 2544
GluI GCNGC 8 cut(s) 210, 747, 855, 1249, 1511, 2444, 2541, 2900
GsaI CCCAGC 2 cut(s) 1639, 2919
GsuI CTGGAG 3 cut(s) 1287, 2539, 2603
HaeII RGCGCY 2 cut(s) 195, 2546
HaeIII GGCC 7 cut(s) 674, 1323, 1850, 2045, 2358, 2777, 2991
HapII CCGG 1 cut(s) 1299
HgaI GACGC 2 cut(s) 1880, 1966
HhaI GCGC 2 cut(s) 194, 2545
Hin6I GCGC 2 cut(s) 192, 2543
HinP1I GCGC 2 cut(s) 192, 2543
HincII GTYRAC 2 cut(s) 318, 1077
HindII GTYRAC 2 cut(s) 318, 1077
HindIII AAGCTT 4 cut(s) 89, 1382, 1400, 2189
HpaII CCGG 1 cut(s) 1299
HphI GGTGA 5 cut(s) 833, 856, 1610, 1705, 2777
Hpy166II GTNNAC 8 cut(s) 156, 318, 711, 1077, 1712, 1820, 1834, 1843
Hpy8I GTNNAC 8 cut(s) 156, 318, 711, 1077, 1712, 1820, 1834, 1843
Hpy99I CGWCG 2 cut(s) 1960, 2729
HpyAV CCTTC 7 cut(s) 662, 925, 1235, 1321, 2167, 2614, 2863
HpyCH4IV ACGT 1 cut(s) 1858
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 911, 962, 1769, 2063, 2360, 2474, 2668
HpySE526I ACGT 1 cut(s) 1858
HspAI GCGC 2 cut(s) 192, 2543
KpnI GGTACC 1 cut(s) 773
Ksp22I TGATCA 3 cut(s) 486, 2211, 2908
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 5 cut(s) 12, 593, 1204, 2584, 2924
Lsp1109I GCAGC 7 cut(s) 196, 758, 866, 1235, 1522, 2552, 2911
LweI GCATC 3 cut(s) 914, 1748, 2249
MabI ACCWGGT 1 cut(s) 1836
MaeI CTAG 6 cut(s) 221, 245, 957, 1223, 2649, 2984
MaeII ACGT 1 cut(s) 1858
MaeIII GTNAC 4 cut(s) 553, 700, 802, 2744
MbiI CCGCTC 1 cut(s) 2443
MfeI CAATTG 2 cut(s) 1874, 2315
MflI RGATCY 5 cut(s) 47, 373, 1270, 2335, 2400
MhlI GDGCHC 5 cut(s) 17, 345, 1209, 1822, 2047
MlsI TGGCCA 1 cut(s) 1850
MluNI TGGCCA 1 cut(s) 1850
MlyI GAGTC 3 cut(s) 301, 1601, 1691
MmeI TCCRAC 3 cut(s) 261, 1298, 1569
Mox20I TGGCCA 1 cut(s) 1850
Mph1103I ATGCAT 3 cut(s) 907, 1540, 2242
MroXI GAANNNNTTC 2 cut(s) 353, 1752
MscI TGGCCA 1 cut(s) 1850
MslI CAYNNNNRTG 4 cut(s) 944, 1556, 2235, 2783
Msp20I TGGCCA 1 cut(s) 1850
MspA1I CMGCKG 1 cut(s) 364
MspCI CTTAAG 1 cut(s) 83
MspI CCGG 1 cut(s) 1299
MspR9I CCNGG 3 cut(s) 1299, 1838, 2993
MunI CAATTG 2 cut(s) 1874, 2315
Mva1269I GAATGC 3 cut(s) 1540, 2330, 2542
MvaI CCWGG 2 cut(s) 1838, 2993
MwoI GCNNNNNNNGC 8 cut(s) 134, 911, 962, 1769, 2063, 2360, 2474, 2668
NciI CCSGG 1 cut(s) 1299
NcoI CCATGG 1 cut(s) 2778
NdeI CATATG 2 cut(s) 281, 598
NheI GCTAGC 1 cut(s) 2648
NlaIV GGNNCC 9 cut(s) 268, 350, 673, 771, 1870, 2044, 2045, 2046, 2295
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 3 cut(s) 553, 802, 2744
NsiI ATGCAT 3 cut(s) 907, 1540, 2242
NspI RCATGY 5 cut(s) 184, 905, 1284, 1555, 2431
PaeR7I CTCGAG 1 cut(s) 638
PceI AGGCCT 1 cut(s) 2358
PciI ACATGT 2 cut(s) 1280, 2427
PciSI GCTCTTC 1 cut(s) 333
PcsI WCGNNNNNNNCGW 1 cut(s) 1368
PctI GAATGC 3 cut(s) 1540, 2330, 2542
PdmI GAANNNNTTC 2 cut(s) 353, 1752
PkrI GCNGC 8 cut(s) 211, 748, 856, 1250, 1512, 2445, 2542, 2901
PleI GAGTC 3 cut(s) 301, 1600, 1690
PpsI GAGTC 3 cut(s) 301, 1600, 1690
Ppu21I YACGTR 1 cut(s) 1859
PpuMI RGGWCCY 1 cut(s) 2294
PscI ACATGT 2 cut(s) 1280, 2427
PshBI ATTAAT 2 cut(s) 696, 780
Psp124BI GAGCTC 2 cut(s) 17, 1209
Psp5II RGGWCCY 1 cut(s) 2294
Psp6I CCWGG 2 cut(s) 1836, 2991
PspFI CCCAGC 2 cut(s) 1635, 2915
PspGI CCWGG 2 cut(s) 1836, 2991
PspN4I GGNNCC 9 cut(s) 268, 350, 673, 771, 1870, 2044, 2045, 2046, 2295
PspOMI GGGCCC 1 cut(s) 2043
PspPI GGNCC 7 cut(s) 267, 672, 1571, 2043, 2044, 2176, 2294
PspPPI RGGWCCY 1 cut(s) 2294
PsuI RGATCY 5 cut(s) 47, 373, 1270, 2335, 2400
PvuII CAGCTG 1 cut(s) 364
RsaI GTAC 4 cut(s) 712, 771, 1857, 2183
RsaNI GTAC 4 cut(s) 711, 770, 1856, 2182
RseI CAYNNNNRTG 4 cut(s) 944, 1556, 2235, 2783
SacI GAGCTC 2 cut(s) 17, 1209
SapI GCTCTTC 1 cut(s) 333
SatI GCNGC 8 cut(s) 210, 747, 855, 1249, 1511, 2444, 2541, 2900
Sau96I GGNCC 7 cut(s) 267, 672, 1571, 2043, 2044, 2176, 2294
SchI GAGTC 3 cut(s) 301, 1601, 1691
ScrFI CCNGG 3 cut(s) 1299, 1838, 2993
SduI GDGCHC 5 cut(s) 17, 345, 1209, 1822, 2047
SexAI ACCWGGT 1 cut(s) 1836
SfaNI GCATC 3 cut(s) 914, 1748, 2249
SfcI CTRYAG 1 cut(s) 2146
Sfr274I CTCGAG 1 cut(s) 638
SinI GGWCC 4 cut(s) 267, 1571, 2176, 2294
SlaI CTCGAG 1 cut(s) 638
SmiMI CAYNNNNRTG 4 cut(s) 944, 1556, 2235, 2783
SmlI CTYRAG 6 cut(s) 83, 330, 638, 1208, 1604, 2615
SmoI CTYRAG 6 cut(s) 83, 330, 638, 1208, 1604, 2615
SpeI ACTAGT 1 cut(s) 2983
SseBI AGGCCT 1 cut(s) 2358
SsiI CCGC 4 cut(s) 759, 1201, 2443, 2738
SspI AATATT 1 cut(s) 494
SspMI CTAG 6 cut(s) 221, 245, 957, 1223, 2649, 2984
SstI GAGCTC 2 cut(s) 17, 1209
StuI AGGCCT 1 cut(s) 2358
StyD4I CCNGG 3 cut(s) 1297, 1836, 2991
StyI CCWWGG 4 cut(s) 262, 270, 1158, 2778
TaiI ACGT 1 cut(s) 1861
TaqI TCGA 2 cut(s) 639, 1362
TatI WGTACW 1 cut(s) 710
TauI GCSGC 1 cut(s) 2446
TscAI CASTG 8 cut(s) 182, 450, 933, 1129, 1243, 1926, 2453, 2797
TseFI GTSAC 3 cut(s) 553, 802, 2744
TseI GCWGC 7 cut(s) 209, 746, 854, 1248, 1510, 2540, 2899
Tsp45I GTSAC 3 cut(s) 553, 802, 2744
TspRI CASTG 8 cut(s) 182, 450, 933, 1129, 1243, 1926, 2453, 2797
Vha464I CTTAAG 1 cut(s) 83
VneI GTGCAC 1 cut(s) 1818
VpaK11BI GGWCC 4 cut(s) 267, 1571, 2176, 2294
VspI ATTAAT 2 cut(s) 696, 780
XapI RAATTY 7 cut(s) 516, 1255, 1609, 1924, 1996, 2819, 2973
XbaI TCTAGA 1 cut(s) 1222
XceI RCATGY 5 cut(s) 184, 905, 1284, 1555, 2431
XhoI CTCGAG 1 cut(s) 638
XmiI GTMKAC 2 cut(s) 155, 1833
XmnI GAANNNNTTC 2 cut(s) 353, 1752
XspI CTAG 6 cut(s) 221, 245, 957, 1223, 2649, 2984
Zsp2I ATGCAT 3 cut(s) 907, 1540, 2242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.