Rroxscaffold_5G00341740

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006689
Physical Location & Seq
Reverse (-)
10355711 .. 10358661
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00341740.1

Sequence Viewer

Length: 2502 bp
ATGCAGAGAATTCAGTCATTTGTATCAGAAGAAGTAGTAGTAAAGGTCACACAGGAGGTCATTGATTCTGTAATAAGACTGTGTGGAGCATATGCCCGAGCTTTGAATTGGGAGTCTTGGGAAGAAACACTCGAGAGGAATAAAACTGACACGGACTTTGAAGGAGCCAATAGTATGAATCATGTTATTAATGTAACAAAGTGTACAATAGAAAAACTATGTGAGATGGGCATTATTGCAGCTAATAATGGCGGAAGTCTGGTACCGATTATTAATATGTCATGGAAAGGTGTGGTCACTTTGCTTCAGCTCGGTGATGGGGTATTAGCAACAAAGGTGAATGTAGCAGCTCTAATTTCAAATCTAATCTCATTGGTTAATGAATCTATAAGACATGCAGCTGAGGCATGGTTTTCATCACTGAAGGAAAACATCTCTGTGTCAGAAGCTAGAAAGGCATTTCTTCCTGTAAAGTTTTATTTGGTTAATGCAGTAAAAATATCTTCCCTATACCCATATCAAGCATATCTGGTGCAAAGTGAGATAACAAAATGTATCCTGATGATGTCAACCTTAAAGATTTCATTGAGCAATGAAAAACTGTTAAAAACTGCCAGTGATGTTTTCACAGAACTCTTGGAGAAGACATCCTTGGATTTGCTTATTGCTCTACTGAACTCAGTTCAAGTGAAGCGGGAGCTTAAGGTTGAGATTCTAGACTATCTCTTCAGTGAAGGAAGCTGCACAAATTCTGTCACTGGAGATCCGACTCCATGTGGGGAAGCATTGCCCGGAGAAAGAACCTTCTTGATCGGCCGTGTTTCATTGTTTCTTAATTTTCTGAGATTTTCTCTCGATCTTGACGATGATGTAAAGCTTGGGATTGCTGGAAAGCTTGGTTGGTTCTTTGATATGTTAGTAAATGAAGAGGTATATTCTTCCATTCTTCTTTTGCAAGTTCCTGTGCTATATGGTTCTGGAAAAACTGTGGAAGTTGTCTGGCAGCCTTTGTTTTCTTCTCTTTTGAATGCATTGCAAATCTACATGCTTGTGGTTTCTTCTGGTCCTGCTTGGAGGGAGTTCGAGTCTTTTCTTCTTGAGAATTTATTTCACCCCCACTTTCTTTGCTGGGAGATTGTAATGGAACTTTGGTGCTTTATGGTTCGCCATGCTGAGTCAGGGATGGTGAGTGGCATTGCTGGTAAACTTTGCTCATTAATGAAATTGGTGGCATCTACTGAATCCGTTCTTGTAGCTGATTCTGCTCTGAGAAAAGTGGCAAGGTCAATCAGCATAATTCTTACTTTTGGTGCACAATCTATGGTAGACCAGGTTTACATGGCCATTGTACGTGATGAGGGAACCCAATTGTCATCAGTTATGCGTCTGGCGTTGTTCATGGAGGGCTTCCCACTGAATTTACTGTCAGACAAGATGAAAAGAATTGCGACGCAAAGGATAATTACTGATTACTGTCTCTTCATTGAAAATTTTGATGAGAAGTCAATGAAATCTCTTGATTGTGGTCTTTTTGGGGCCCCAGTTTTTGCCTTGTCAGCTTCGTTGCAGTCTCTTCAGATCAGCACATCTGATATCAATGTGAAGACCTTGAAGTTTTTAGTTGCCATCATTCATAACTACAGAGTTTGTGAAGATCAGCTAATGAAGGACCAGTACCGTAAGCTTCTGAGTGAAACATTAGTGATCATCTCAAATATGAAGCACTTATATGCATCTCTTGAAATGGAGAAAGTAATCTGTGAGCTTGAACACCTGTTTATCTCGGGACCTGCTGTTTCTGATACCCAATTGTATGAATGCAAACCAGATCTGGCTCTTTTCATGGCAGGCCTTGCTCACATGGATATCAAAGAAGCTGATGAAAGTTCCAAGATCTCTGCTCTATGGGATTTGTATCACATGTTATTGGGAGAGCGGCACTGGGCATTTATTCATTTGGCAATTACAGCATTTGGATACTTTTCTGCTCGGACAAATTGCAATAAGCTCTGGAGATTTGTGCCTCAGAATGCAGCACTTTCGTATGATCTGACTTCTGGAAATGAGGCAAATCTGGAGCGATTTATGTCTGAGTTCAAGGTTTTTCTTGAGAAGGAAACGGCACTTCTCACAATAACGGCTAGCTCTGACCAGCTTGGTCTGCTTGTGAGGGAGGGTCTAAAACTAAAAGAAATGTTCCAAAAGATTTCAAATGTCGTCGTTGAGACTGCAGAATGTGACTACATGGAAATTGATGGTGAAAAGCAGATCATGGAGATGAACAGTGGAAAACAATCCAATAGGAAAAGGAAATTTCCTGATGGAATTGAAAAGGGAATGGAACTGCTACAAAGTGGTATGAAGGTTATTGTGGATAGTATTTCTCAGTTGCAGCAAAATGATCAACCCAGCTCCAATGAACTACCCGACGACTTCTTGACCACCTTTTCTCGCCTTGAAGCTGAAATTTCTCAACTAGTTGGCCTGGCTGGTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

833

Amino Acids

93.3

Weight (kDa)

5.09

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 90 - 670 7.8e-150 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1798
Acc65I GGTACC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 262
AccBSI CCGCTC 1 cut(s) 1936
AccI GTMKAC 1 cut(s) 1326
AciI CCGC 3 cut(s) 252, 694, 1936
AclWI GGATC 1 cut(s) 758
AcoI YGGCCR 2 cut(s) 814, 1341
AcsI RAATTY 7 cut(s) 9, 748, 1102, 1417, 1489, 2312, 2466
AcuI CTGAAG 4 cut(s) 290, 443, 712, 1559
AfaI GTAC 4 cut(s) 205, 264, 1350, 1676
AflII CTTAAG 1 cut(s) 701
AflIII ACRYGT 1 cut(s) 1920
AhlI ACTAGT 1 cut(s) 2476
AjnI CCWGG 2 cut(s) 1329, 2484
AjuI GAANNNNNNNTTGG 4 cut(s) 635, 667, 883, 915
Alw21I GWGCWC 1 cut(s) 1315
Alw26I GTCTC 3 cut(s) 1481, 1575, 2219
Alw44I GTGCAC 1 cut(s) 1311
AlwI GGATC 1 cut(s) 758
Ama87I CYCGRG 3 cut(s) 96, 131, 1783
AoxI GGCC 5 cut(s) 814, 1341, 1536, 1849, 2482
ApaI GGGCCC 1 cut(s) 1540
ApaLI GTGCAC 1 cut(s) 1311
ApeKI GCWGC 7 cut(s) 239, 347, 398, 741, 1003, 2033, 2392
ApoI RAATTY 7 cut(s) 9, 748, 1102, 1417, 1489, 2312, 2466
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
AseI ATTAAT 3 cut(s) 189, 273, 1217
Asp700I GAANNNNTTC 1 cut(s) 1245
Asp718I GGTACC 1 cut(s) 262
AspS9I GGNCC 5 cut(s) 1064, 1536, 1537, 1669, 1787
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 5 cut(s) 326, 349, 1103, 1198, 2270
AsuNHI GCTAGC 1 cut(s) 2141
AvaI CYCGRG 3 cut(s) 96, 131, 1783
AvaII GGWCC 3 cut(s) 1064, 1669, 1787
BaeGI GKGCMC 2 cut(s) 1315, 1540
BalI TGGCCA 1 cut(s) 1343
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 1540
BarI GAAGNNNNNNTAC 2 cut(s) 1658, 1690
BbsI GAAGAC 2 cut(s) 650, 1610
Bbv12I GWGCWC 1 cut(s) 1315
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 7 cut(s) 251, 359, 410, 728, 1015, 2045, 2404
BccI CCATC 6 cut(s) 220, 311, 1177, 1634, 2249, 2315
BceAI ACGGC 3 cut(s) 801, 2136, 2154
BcgI CGANNNNNNTGC 2 cut(s) 2022, 2056
BciT130I CCWGG 2 cut(s) 1331, 2486
BciVI GTATCC 2 cut(s) 566, 1970
BclI TGATCA 2 cut(s) 1704, 2401
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 3 cut(s) 1481, 1575, 2219
BcuI ACTAGT 1 cut(s) 2476
BfaI CTAG 4 cut(s) 450, 716, 2142, 2477
BfmI CTRYAG 2 cut(s) 1639, 2229
BfrI CTTAAG 1 cut(s) 701
BfuAI ACCTGC 1 cut(s) 1798
BfuI GTATCC 2 cut(s) 566, 1970
BglII AGATCT 2 cut(s) 1828, 1893
BisI GCNGC 8 cut(s) 240, 348, 399, 742, 1004, 1937, 2034, 2393
BlsI GCNGC 8 cut(s) 241, 349, 400, 743, 1005, 1938, 2035, 2394
Bme1390I CCNGG 3 cut(s) 792, 1331, 2486
Bme18I GGWCC 3 cut(s) 1064, 1669, 1787
BmeT110I CYCGRG 3 cut(s) 96, 131, 1783
BmgT120I GGNCC 5 cut(s) 1064, 1536, 1537, 1669, 1787
BmiI GGNNCC 7 cut(s) 166, 264, 1363, 1537, 1538, 1539, 1788
BmrFI CCNGG 3 cut(s) 792, 1331, 2486
BmrI ACTGGG 2 cut(s) 1535, 1951
BmsI GCATC 2 cut(s) 1241, 1742
BmtI GCTAGC 1 cut(s) 2145
BmuI ACTGGG 2 cut(s) 1535, 1951
BpiI GAAGAC 2 cut(s) 650, 1610
BplI GAGNNNNNCTC 2 cut(s) 835, 867
BpmI CTGGAG 3 cut(s) 780, 2032, 2096
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 2 cut(s) 1118, 2129
BpuMI CCSGG 1 cut(s) 792
BsaAI YACGTR 1 cut(s) 1352
BsaBI GATNNNNATC 1 cut(s) 1914
BsaJI CCNNGG 1 cut(s) 651
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bse1I ACTGG 5 cut(s) 615, 763, 1541, 1672, 1946
Bse3DI GCAATG 4 cut(s) 598, 785, 1031, 1194
Bse8I GATNNNNATC 1 cut(s) 1914
BseBI CCWGG 2 cut(s) 1331, 2486
BseDI CCNNGG 1 cut(s) 651
BseGI GGATG 2 cut(s) 647, 1188
BseJI GATNNNNATC 1 cut(s) 1914
BseMI GCAATG 4 cut(s) 598, 785, 1031, 1194
BseMII CTCAG 9 cut(s) 393, 693, 833, 1164, 1259, 1679, 2039, 2082, 2399
BseNI ACTGG 5 cut(s) 615, 763, 1541, 1672, 1946
BseSI GKGCMC 2 cut(s) 1315, 1540
BseX3I CGGCCG 1 cut(s) 814
BseXI GCAGC 7 cut(s) 251, 359, 410, 728, 1015, 2045, 2404
BseYI CCCAGC 2 cut(s) 1128, 2408
BsgI GTGCAG 1 cut(s) 727
Bsh1285I CGRYCG 1 cut(s) 817
BshFI GGCC 5 cut(s) 816, 1343, 1538, 1851, 2484
BshNI GGYRCC 1 cut(s) 262
BsiEI CGRYCG 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 1315
BsiHKCI CYCGRG 3 cut(s) 96, 131, 1783
BsiSI CCGG 1 cut(s) 792
BslFI GGGAC 1 cut(s) 1800
BsmAI GTCTC 3 cut(s) 1481, 1575, 2219
BsmFI GGGAC 1 cut(s) 1800
BsmI GAATGC 3 cut(s) 1033, 1823, 2035
BsnI GGCC 5 cut(s) 816, 1343, 1538, 1851, 2484
BsoBI CYCGRG 3 cut(s) 96, 131, 1783
Bsp120I GGGCCC 1 cut(s) 1536
Bsp1286I GDGCHC 2 cut(s) 1315, 1540
Bsp1407I TGTACA 1 cut(s) 203
BspACI CCGC 3 cut(s) 252, 694, 1936
BspANI GGCC 5 cut(s) 816, 1343, 1538, 1851, 2484
BspCNI CTCAG 9 cut(s) 394, 692, 834, 1165, 1260, 1680, 2038, 2083, 2398
BspLI GGNNCC 7 cut(s) 166, 264, 1363, 1537, 1538, 1539, 1788
BspMAI CTGCAG 1 cut(s) 2233
BspMI ACCTGC 1 cut(s) 1798
BspOI GCTAGC 1 cut(s) 2145
BspPI GGATC 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 262
BspTI CTTAAG 1 cut(s) 701
BsrBI CCGCTC 1 cut(s) 1936
BsrDI GCAATG 4 cut(s) 598, 785, 1031, 1194
BsrGI TGTACA 1 cut(s) 203
BsrI ACTGG 5 cut(s) 615, 763, 1541, 1672, 1946
BssECI CCNNGG 1 cut(s) 651
BssT1I CCWWGG 1 cut(s) 651
Bst2UI CCWGG 2 cut(s) 1331, 2486
Bst4CI ACNGT 7 cut(s) 81, 603, 988, 1425, 1475, 1679, 2285
Bst6I CTCTTC 4 cut(s) 731, 921, 1484, 1578
BstAFI CTTAAG 1 cut(s) 701
BstAPI GCANNNNNTGC 1 cut(s) 1853
BstAUI TGTACA 1 cut(s) 203
BstBAI YACGTR 1 cut(s) 1352
BstC8I GCNNGC 2 cut(s) 1849, 2143
BstDEI CTNAG 9 cut(s) 402, 679, 842, 1173, 1268, 1688, 2025, 2091, 2385
BstF5I GGATG 2 cut(s) 647, 1188
BstMAI GTCTC 3 cut(s) 1481, 1575, 2219
BstMCI CGRYCG 1 cut(s) 817
BstMWI GCNNNNNNNGC 7 cut(s) 404, 455, 1262, 1556, 1853, 1967, 2161
BstNI CCWGG 2 cut(s) 1331, 2486
BstNSI RCATGY 3 cut(s) 398, 1048, 1924
BstSCI CCNGG 3 cut(s) 790, 1329, 2484
BstSFI CTRYAG 2 cut(s) 1639, 2229
BstSLI GKGCMC 2 cut(s) 1315, 1540
BstV1I GCAGC 7 cut(s) 251, 359, 410, 728, 1015, 2045, 2404
BstV2I GAAGAC 2 cut(s) 650, 1610
BstX2I RGATCY 3 cut(s) 763, 1828, 1893
BstYI RGATCY 3 cut(s) 763, 1828, 1893
BstZI CGGCCG 1 cut(s) 814
BsuI GTATCC 2 cut(s) 566, 1970
BsuRI GGCC 5 cut(s) 816, 1343, 1538, 1851, 2484
BtsCI GGATG 2 cut(s) 647, 1188
BtsIMutI CAGTG 7 cut(s) 419, 622, 736, 756, 1412, 1939, 2290
BveI ACCTGC 1 cut(s) 1798
Cac8I GCNNGC 2 cut(s) 1849, 2143
Cfr13I GGNCC 5 cut(s) 1064, 1536, 1537, 1669, 1787
CseI GACGC 2 cut(s) 1373, 1459
CsiI ACCWGGT 1 cut(s) 1329
Csp6I GTAC 4 cut(s) 204, 263, 1349, 1675
CspCI CAANNNNNGTGG 2 cut(s) 1106, 1141
CviQI GTAC 4 cut(s) 204, 263, 1349, 1675
DdeI CTNAG 9 cut(s) 402, 679, 842, 1173, 1268, 1688, 2025, 2091, 2385
EaeI YGGCCR 2 cut(s) 814, 1341
EagI CGGCCG 1 cut(s) 814
Eam1104I CTCTTC 4 cut(s) 731, 921, 1484, 1578
EarI CTCTTC 4 cut(s) 731, 921, 1484, 1578
EciI GGCGGA 1 cut(s) 267
EclXI CGGCCG 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 651
Eco147I AGGCCT 1 cut(s) 1851
Eco24I GRGCYC 1 cut(s) 1540
Eco32I GATATC 2 cut(s) 1594, 1867
Eco47I GGWCC 3 cut(s) 1064, 1669, 1787
Eco52I CGGCCG 1 cut(s) 814
Eco57I CTGAAG 4 cut(s) 290, 443, 712, 1559
Eco88I CYCGRG 3 cut(s) 96, 131, 1783
EcoO109I RGGNCCY 3 cut(s) 1536, 1537, 1787
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 2 cut(s) 1329, 2484
EcoRV GATATC 2 cut(s) 1594, 1867
EcoT14I CCWWGG 1 cut(s) 651
EcoT22I ATGCAT 2 cut(s) 1033, 1735
EcoT38I GRGCYC 1 cut(s) 1540
ErhI CCWWGG 1 cut(s) 651
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FaqI GGGAC 1 cut(s) 1800
FauI CCCGC 1 cut(s) 687
FauNDI CATATG 1 cut(s) 91
FbaI TGATCA 2 cut(s) 1704, 2401
FblI GTMKAC 1 cut(s) 1326
Fnu4HI GCNGC 8 cut(s) 240, 348, 399, 742, 1004, 1937, 2034, 2393
FokI GGATG 2 cut(s) 634, 1195
FriOI GRGCYC 1 cut(s) 1540
Fsp4HI GCNGC 8 cut(s) 240, 348, 399, 742, 1004, 1937, 2034, 2393
FspBI CTAG 4 cut(s) 450, 716, 2142, 2477
GluI GCNGC 8 cut(s) 240, 348, 399, 742, 1004, 1937, 2034, 2393
GsaI CCCAGC 2 cut(s) 1132, 2412
GsuI CTGGAG 3 cut(s) 780, 2032, 2096
HaeIII GGCC 5 cut(s) 816, 1343, 1538, 1851, 2484
HapII CCGG 1 cut(s) 792
HgaI GACGC 2 cut(s) 1373, 1459
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HindIII AAGCTT 3 cut(s) 875, 893, 1682
HpaII CCGG 1 cut(s) 792
HphI GGTGA 5 cut(s) 326, 349, 1103, 1198, 2270
Hpy166II GTNNAC 6 cut(s) 204, 570, 1205, 1313, 1327, 1336
Hpy8I GTNNAC 6 cut(s) 204, 570, 1205, 1313, 1327, 1336
Hpy99I CGWCG 3 cut(s) 1453, 2222, 2432
HpyAV CCTTC 7 cut(s) 155, 418, 728, 814, 1660, 2107, 2356
HpyCH4III ACNGT 7 cut(s) 81, 603, 988, 1425, 1475, 1679, 2285
HpyCH4IV ACGT 1 cut(s) 1351
HpyF10VI GCNNNNNNNGC 7 cut(s) 404, 455, 1262, 1556, 1853, 1967, 2161
HpyF3I CTNAG 9 cut(s) 402, 679, 842, 1173, 1268, 1688, 2025, 2091, 2385
HpySE526I ACGT 1 cut(s) 1351
KpnI GGTACC 1 cut(s) 266
Ksp22I TGATCA 2 cut(s) 1704, 2401
LmnI GCTCC 5 cut(s) 86, 164, 697, 2077, 2417
Lsp1109I GCAGC 7 cut(s) 251, 359, 410, 728, 1015, 2045, 2404
LweI GCATC 2 cut(s) 1241, 1742
MabI ACCWGGT 1 cut(s) 1329
MaeI CTAG 4 cut(s) 450, 716, 2142, 2477
MaeII ACGT 1 cut(s) 1351
MaeIII GTNAC 5 cut(s) 46, 193, 295, 754, 2237
MbiI CCGCTC 1 cut(s) 1936
MfeI CAATTG 2 cut(s) 1367, 1808
MflI RGATCY 3 cut(s) 763, 1828, 1893
MhlI GDGCHC 2 cut(s) 1315, 1540
MlsI TGGCCA 1 cut(s) 1343
MluNI TGGCCA 1 cut(s) 1343
MlyI GAGTC 4 cut(s) 122, 763, 1094, 1184
MmeI TCCRAC 1 cut(s) 791
Mox20I TGGCCA 1 cut(s) 1343
Mph1103I ATGCAT 2 cut(s) 1033, 1735
MroXI GAANNNNTTC 1 cut(s) 1245
MscI TGGCCA 1 cut(s) 1343
MseI TTAA 9 cut(s) 189, 273, 378, 486, 575, 605, 702, 834, 1217
MslI CAYNNNNRTG 4 cut(s) 437, 1049, 1728, 2276
Msp20I TGGCCA 1 cut(s) 1343
MspA1I CMGCKG 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 701
MspI CCGG 1 cut(s) 792
MspR9I CCNGG 3 cut(s) 792, 1331, 2486
MunI CAATTG 2 cut(s) 1367, 1808
Mva1269I GAATGC 3 cut(s) 1033, 1823, 2035
MvaI CCWGG 2 cut(s) 1331, 2486
MwoI GCNNNNNNNGC 7 cut(s) 404, 455, 1262, 1556, 1853, 1967, 2161
NciI CCSGG 1 cut(s) 792
NdeI CATATG 1 cut(s) 91
NheI GCTAGC 1 cut(s) 2141
NlaIV GGNNCC 7 cut(s) 166, 264, 1363, 1537, 1538, 1539, 1788
NmuCI GTSAC 4 cut(s) 46, 295, 754, 2237
NsiI ATGCAT 2 cut(s) 1033, 1735
NspI RCATGY 3 cut(s) 398, 1048, 1924
PaeR7I CTCGAG 1 cut(s) 131
PceI AGGCCT 1 cut(s) 1851
PciI ACATGT 1 cut(s) 1920
PcsI WCGNNNNNNNCGW 1 cut(s) 861
PctI GAATGC 3 cut(s) 1033, 1823, 2035
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 6 cut(s) 65, 178, 383, 712, 1241, 1259
PkrI GCNGC 8 cut(s) 241, 349, 400, 743, 1005, 1938, 2035, 2394
PleI GAGTC 4 cut(s) 121, 763, 1093, 1183
PpsI GAGTC 4 cut(s) 121, 763, 1093, 1183
Ppu21I YACGTR 1 cut(s) 1352
PpuMI RGGWCCY 1 cut(s) 1787
PscI ACATGT 1 cut(s) 1920
PshBI ATTAAT 3 cut(s) 189, 273, 1217
Psp5II RGGWCCY 1 cut(s) 1787
Psp6I CCWGG 2 cut(s) 1329, 2484
PspFI CCCAGC 2 cut(s) 1128, 2408
PspGI CCWGG 2 cut(s) 1329, 2484
PspN4I GGNNCC 7 cut(s) 166, 264, 1363, 1537, 1538, 1539, 1788
PspOMI GGGCCC 1 cut(s) 1536
PspPI GGNCC 5 cut(s) 1064, 1536, 1537, 1669, 1787
PspPPI RGGWCCY 1 cut(s) 1787
PstI CTGCAG 1 cut(s) 2233
PsuI RGATCY 3 cut(s) 763, 1828, 1893
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 4 cut(s) 205, 264, 1350, 1676
RsaNI GTAC 4 cut(s) 204, 263, 1349, 1675
RseI CAYNNNNRTG 4 cut(s) 437, 1049, 1728, 2276
SaqAI TTAA 9 cut(s) 189, 273, 378, 486, 575, 605, 702, 834, 1217
SatI GCNGC 8 cut(s) 240, 348, 399, 742, 1004, 1937, 2034, 2393
Sau96I GGNCC 5 cut(s) 1064, 1536, 1537, 1669, 1787
SchI GAGTC 4 cut(s) 122, 763, 1094, 1184
ScrFI CCNGG 3 cut(s) 792, 1331, 2486
SduI GDGCHC 2 cut(s) 1315, 1540
SexAI ACCWGGT 1 cut(s) 1329
SfaNI GCATC 2 cut(s) 1241, 1742
SfcI CTRYAG 2 cut(s) 1639, 2229
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 3 cut(s) 1064, 1669, 1787
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 4 cut(s) 437, 1049, 1728, 2276
SmlI CTYRAG 4 cut(s) 131, 701, 1097, 2108
SmoI CTYRAG 4 cut(s) 131, 701, 1097, 2108
SpeI ACTAGT 1 cut(s) 2476
SseBI AGGCCT 1 cut(s) 1851
SsiI CCGC 3 cut(s) 252, 694, 1936
SspMI CTAG 4 cut(s) 450, 716, 2142, 2477
StuI AGGCCT 1 cut(s) 1851
StyD4I CCNGG 3 cut(s) 790, 1329, 2484
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 7 cut(s) 81, 603, 988, 1425, 1475, 1679, 2285
TaiI ACGT 1 cut(s) 1354
TaqI TCGA 3 cut(s) 132, 855, 1083
TatI WGTACW 1 cut(s) 203
TauI GCSGC 1 cut(s) 1939
TfiI GAWTC 6 cut(s) 65, 178, 383, 712, 1241, 1259
Tru1I TTAA 9 cut(s) 189, 273, 378, 486, 575, 605, 702, 834, 1217
Tru9I TTAA 9 cut(s) 189, 273, 378, 486, 575, 605, 702, 834, 1217
TscAI CASTG 7 cut(s) 426, 622, 736, 763, 1419, 1946, 2290
TseFI GTSAC 4 cut(s) 46, 295, 754, 2237
TseI GCWGC 7 cut(s) 239, 347, 398, 741, 1003, 2033, 2392
Tsp45I GTSAC 4 cut(s) 46, 295, 754, 2237
TspGWI ACGGA 2 cut(s) 167, 1234
TspRI CASTG 7 cut(s) 426, 622, 736, 763, 1419, 1946, 2290
Vha464I CTTAAG 1 cut(s) 701
VneI GTGCAC 1 cut(s) 1311
VpaK11BI GGWCC 3 cut(s) 1064, 1669, 1787
VspI ATTAAT 3 cut(s) 189, 273, 1217
XapI RAATTY 7 cut(s) 9, 748, 1102, 1417, 1489, 2312, 2466
XbaI TCTAGA 1 cut(s) 715
XceI RCATGY 3 cut(s) 398, 1048, 1924
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 1326
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 4 cut(s) 450, 716, 2142, 2477
Zsp2I ATGCAT 2 cut(s) 1033, 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.