MD00G1101100.v1.1

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006689
Physical Location & Seq
Forward (+)
21434503 .. 21440611
6109 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1101100.v1.1.491

Sequence Viewer

Length: 3039 bp
ATGGAAGGAGGGAGCTCAAGCAGCGAATTGCAGAGCATAGAAGACGCCATTAGATCCTCGGACGTAGTGGAGAATCGTATTGAACTACTGGGGAAGCTTGGTGAGTTGAACATTACAGAGAAATCTGAATTGGCATCCCTTGCGAAATGTCTCACATATACTTGCTTGGATGTAAGCCAGTGCATGTTAAACAAGGCTATATTACATGTGGCTGTAAAACACCTAGAATCTGACATATCCAACGCTCTAGCACATTTCCTTGCCCTTGGGACCAAGGCTAACATATGGTGTGGTAAACACCTAAAAATGACTCTTATGTCGAGTGTGGAGTCTCAAGAGGAAGAGCACGACAATCTGTTCTTTGAGCTGCTTCTGGATCTTCTGAGCTTCTCTGGTGTTAGTTTCTCAGCTTTGGCAAGATTTCCTGTTTCTACCAACAAGCTGTCAATGGATATTGTTGAGAAATTCTTAGTGGAACAGTTAAACTTGATCAAAGATTCAATATCAGAGATTAAGAGAATTGAGCCATTTGAATCTGTAGGAAAGGTCATGTTGGAGGTCATTGATGCTGTAATAAGACTATGTGGAGCATATGCTCGAGCTGTAAATTGGGAGTCTTGGGAAGAAAAGCTTGAGAGGGACAAAACTGGAACGCATTTTGAAGGGGTCAGTAATATGAATCACGTTATTACTGTAATAAAGTATACAATAGAAAAATTGTGTGAGATAGGCGTTGTTGCTGCTAATAATGGAGGCAGTCTGGTAAAGGTTCTCAATTTTACATGGAAAGGTGTAGTCAGTCTACTTCAGCTTGGTGAGGGGGTATTCGCAACAAAGGTGAATGTAGCTGATATAGTTTCAAATCTAATCTCACTGGTCAATGAATCTTTAAAATGCGCAGCTGAGGCTTGGTCATCGTCTTTGAATGATGCCATTTCAGTGACAGAAGCTAGGAAGACATTTCTTCCAATAAAGTTTTATCTGATTAATGCAATAAAAATATCTACCCTGTATCCGTGTCAAGCATATCTGGTACAAAGGGAGATAACAAATTGCATCCTGATGATCTCAACCTTCAAAATTTCATTGAGCAATGAAAAACTGTTAAAAACCGCCGCTGAAGTTTTCGTAGAACTCTTGGAGAAAGCATCCTTGGATTTACTATTTTCGTTACTGAATTCATCTCAAGTGAAGCAGGAGTTCAAGGTTGAGATTCTAGATTCTCTGTTCGGTAAAGGAAGCTACACAGATACTAGCTCTGGAGATATGAGTAAATTTAATAAGATAAGTTCGTTGGATGAAATTATTTCTTTATTTGGGGAAGCATTGCCTGGAGAAAGAGCCTTGTTGCTTGGTCGTGTTTCGTTATTTCTTAGTTTGTTGAAATTTTCTGTTGATCTTGAAGAAGATGTAAAACTTGGGATTACTAGAAAGCTTGGTTGGTTCTTGGATATATTAATTGATGAAGAGGTATATTCTTCCATTTTGCTTTTGCAAGTTCCCGTGTTATATGGTTCTGGAGAAACTGTGGTAGTTGCCTGGCAGCCTATGTTTTCTTCACTTTTGAATGCATTGGAAATCTTCATGCTTGTGGTCTCTTCGACTCCTGTTTGGAGGGAGTTGGAGGCTTTCCTTCTTGAGAATATATTTCATCCTCACTTCCTTTGCTGGGAGGTTGTGATGGAACTTTGGTGCTTTATGCTGCGGTATGCTGAGCCAGGGACGGTGAGTGGCATTATTGGTAAACTCTGCTCATTACTGAAGTTAGTGGCATCTTCTGAATCAGTTCTTGTACCTGGTTCTGCTCTGCGAAAATTGGCAAGATCAATCAGCATGCTTCTTACTTTTGGCTCACCATCTATCGTAGACCAGGTTTACATGTCCATTGTTAGTGATGAGGGAATTCAGTCGTCATCAGTTATACGTCTAGCCTTATTCATGGAAGGATTTCCACTGAATTTACTCTCTGACAAGTTGAAAAGTATTGCAACTCAAAGAATCCTTACTGATTACTATGTCTTCATTGAAAATTTTGATGACAAATCTATGAGATCTTCTGGTTCCGGTGTTTTTGGCGTCCCAGTGTTTGCTTTGTCTGCTTCATTGGAGTCTCTCCGGATCAGTATATCCGATATCGATGTGAAGACTCTGAAGCTTTTAGTTTCTATCATTCATAACTACATAGTGTCTTCAGACAAACTAATGAAGGACCACTACCGTAAGCTTTTGAGTGAAACATTGGTAATCATCTCAAAAATGAAGCACCTATATGCATCTGACGAAATGGAGAAAGTAATCTTTGAGCTCAAAAACCTGTTTATCTCAGGATCTGGTGCAGCTTCTGATACACAATTGTATGAATGCAAACCAAATCTGGCTCTTTTCATGGCAGGCCTTGCTCATATGGAAATCAATGAAACTAATGAAAGTGCAAAGATCTCTACTCTCTGGGAGTTGTATCACATGTTGTTGAGTGAACGGCATTGGGCATTTATTCACTTGGCTATTACAGCATTTGGATACTTTTCTGCTCGGACTGGTTGCAATGAGCTTTGGAGATTTGTGCCGGAGACTGCAGCGCTTTCATATGATTTAGAATCTGGAAATGAGGCAACTGTGGAGAGATTCATGTCTGAGTTTAAGATATATCTCGAGAAGGAAACAGCGCTTCTTACAACGACACCTTGTTCTGACCAGCTTGGTCTGCTTGCGAGGGAGGGTCTAACTCTGAAAGAAATGTTTCAGAAGATTTCAGATACCATTGTGGATACTTCAGAACGTGACAACATGGAAATCGACTGTGAAAAGCAAACGGAGATCAATGGCGGGAAGCAAACCAATAAGAAAAGGAAACTTCCGGATGGAATTAGGAAGGGAATGGAATTGCTAGGGAGTGGTATGAAGGTTATTGTTGATGGTCTGTCCCAGTGGCAGCAAACGCAATTTTGCTCCAATGAACTTCACGACAAGTTCTTGACGAGCTTTTCTCGCCTTGAAGATGAAATTTCTCAACTAGTTGGCCTGGCTGGTAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

1013

Amino Acids

112.81

Weight (kDa)

5.09

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 256 - 851 3.5e-154 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
Acc16I TGCGCA 1 cut(s) 898
AccI GTMKAC 3 cut(s) 704, 802, 1866
AccIII TCCGGA 2 cut(s) 2115, 2857
AciI CCGC 4 cut(s) 1113, 1116, 1705, 2826
AclWI GGATC 4 cut(s) 48, 384, 2126, 2335
AcsI RAATTY 9 cut(s) 464, 1080, 1177, 1274, 1385, 1902, 1957, 2029, 3003
AcuI CTGAAG 6 cut(s) 791, 1140, 1781, 2171, 2175, 2757
AcyI GRCGYC 2 cut(s) 45, 2076
AfaI GTAC 2 cut(s) 1035, 1794
AfeI AGCGCT 2 cut(s) 2580, 2667
AfiI CCNNNNNNNGG 1 cut(s) 1669
AflIII ACRYGT 3 cut(s) 205, 1878, 2463
AhlI ACTAGT 1 cut(s) 3013
AjnI CCWGG 6 cut(s) 1330, 1538, 1717, 1795, 1869, 3021
AjuI GAANNNNNNNTTGG 6 cut(s) 113, 145, 1136, 1168, 1423, 1455
Alw21I GWGCWC 3 cut(s) 17, 348, 2307
Alw26I GTCTC 5 cut(s) 155, 336, 1600, 2115, 2564
AlwI GGATC 4 cut(s) 48, 384, 2126, 2335
AlwNI CAGNNNCTG 2 cut(s) 2330, 2342
Ama87I CYCGRG 2 cut(s) 597, 2651
Aor13HI TCCGGA 2 cut(s) 2115, 2857
Aor51HI AGCGCT 2 cut(s) 2580, 2667
AoxI GGCC 2 cut(s) 2392, 3019
ApeKI GCWGC 9 cut(s) 21, 367, 740, 899, 1543, 1702, 2336, 2576, 2932
ApoI RAATTY 9 cut(s) 464, 1080, 1177, 1274, 1385, 1902, 1957, 2029, 3003
AseI ATTAAT 2 cut(s) 987, 1457
Asp700I GAANNNNTTC 3 cut(s) 1785, 1947, 2739
AspLEI GCGC 3 cut(s) 899, 2581, 2668
AspS9I GGNCC 2 cut(s) 270, 2209
AsuHPI GGTGA 5 cut(s) 113, 827, 850, 1738, 1845
AvaI CYCGRG 2 cut(s) 597, 2651
AvaII GGWCC 2 cut(s) 270, 2209
BaeI ACNNNNGTAYC 2 cut(s) 2748, 2781
BanII GRGCYC 2 cut(s) 17, 2307
BarI GAAGNNNNNNTAC 2 cut(s) 2198, 2230
BbsI GAAGAC 5 cut(s) 48, 962, 2011, 2150, 2181
Bbv12I GWGCWC 3 cut(s) 17, 348, 2307
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 9 cut(s) 33, 354, 727, 911, 1555, 1689, 2348, 2588, 2944
BccI CCATC 4 cut(s) 1675, 1864, 2855, 2909
BceAI ACGGC 1 cut(s) 2495
BciT130I CCWGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
BciVI GTATCC 3 cut(s) 1023, 2513, 2761
BclI TGATCA 1 cut(s) 489
BcoDI GTCTC 5 cut(s) 155, 336, 1600, 2115, 2564
BcuI ACTAGT 1 cut(s) 3013
BfaI CTAG 9 cut(s) 224, 248, 951, 1217, 1254, 1428, 1928, 2888, 3014
BfmI CTRYAG 2 cut(s) 537, 2574
BfoI RGCGCY 2 cut(s) 2582, 2669
BfuI GTATCC 3 cut(s) 1023, 2513, 2761
BglII AGATCT 2 cut(s) 2051, 2436
BlpI GCTNAGC 1 cut(s) 1713
Bme1390I CCNGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
Bme18I GGWCC 2 cut(s) 270, 2209
BmeT110I CYCGRG 2 cut(s) 597, 2651
BmgT120I GGNCC 2 cut(s) 270, 2209
BmiI GGNNCC 2 cut(s) 271, 2062
BmrFI CCNGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
BmrI ACTGGG 3 cut(s) 98, 2075, 2920
BmsI GCATC 7 cut(s) 143, 556, 919, 1065, 1157, 1781, 2282
BmuI ACTGGG 3 cut(s) 98, 2075, 2920
BpiI GAAGAC 5 cut(s) 48, 962, 2011, 2150, 2181
BplI GAGNNNNNCTC 2 cut(s) 2971, 3003
BpmI CTGGAG 3 cut(s) 1281, 1353, 1539
Bpu10I CCTNAGC 1 cut(s) 903
Bpu1102I GCTNAGC 1 cut(s) 1713
BpuEI CTTGAG 4 cut(s) 318, 653, 1170, 1658
Bsa29I ATCGAT 1 cut(s) 2136
BsaHI GRCGYC 2 cut(s) 45, 2076
BsaI GGTCTC 1 cut(s) 1600
BsaJI CCNNGG 5 cut(s) 57, 265, 273, 1152, 1718
BsaWI WCCGGW 3 cut(s) 2063, 2115, 2857
BsaXI ACNNNNNCTCC 2 cut(s) 1512, 1542
Bsc4I CCNNNNNNNGG 1 cut(s) 1669
Bse1I ACTGG 7 cut(s) 93, 178, 652, 879, 2081, 2542, 2926
Bse3DI GCAATG 3 cut(s) 1099, 1325, 2551
BseAI TCCGGA 2 cut(s) 2115, 2857
BseBI CCWGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
BseCI ATCGAT 1 cut(s) 2136
BseDI CCNNGG 5 cut(s) 57, 265, 273, 1152, 1718
BseGI GGATG 7 cut(s) 134, 175, 1056, 1148, 1303, 1651, 2866
BseLI CCNNNNNNNGG 1 cut(s) 1669
BseMI GCAATG 3 cut(s) 1099, 1325, 2551
BseMII CTCAG 6 cut(s) 374, 420, 894, 1704, 2337, 2625
BseNI ACTGG 7 cut(s) 93, 178, 652, 879, 2081, 2542, 2926
BseXI GCAGC 9 cut(s) 33, 354, 727, 911, 1555, 1689, 2348, 2588, 2944
BseYI CCCAGC 1 cut(s) 1668
BsgI GTGCAG 1 cut(s) 2355
BshFI GGCC 2 cut(s) 2394, 3021
BshVI ATCGAT 1 cut(s) 2136
BsiHKAI GWGCWC 3 cut(s) 17, 348, 2307
BsiHKCI CYCGRG 2 cut(s) 597, 2651
BsiSI CCGG 4 cut(s) 2064, 2116, 2567, 2858
BslFI GGGAC 5 cut(s) 283, 653, 1735, 2063, 2908
BslI CCNNNNNNNGG 1 cut(s) 1669
BsmAI GTCTC 5 cut(s) 155, 336, 1600, 2115, 2564
BsmFI GGGAC 5 cut(s) 283, 653, 1735, 2063, 2908
BsmI GAATGC 2 cut(s) 1573, 2366
BsnI GGCC 2 cut(s) 2394, 3021
Bso31I GGTCTC 1 cut(s) 1600
BsoBI CYCGRG 2 cut(s) 597, 2651
Bsp1286I GDGCHC 3 cut(s) 17, 348, 2307
Bsp13I TCCGGA 2 cut(s) 2115, 2857
Bsp1720I GCTNAGC 1 cut(s) 1713
BspACI CCGC 4 cut(s) 1113, 1116, 1705, 2826
BspANI GGCC 2 cut(s) 2394, 3021
BspCNI CTCAG 6 cut(s) 375, 419, 895, 1705, 2336, 2626
BspDI ATCGAT 1 cut(s) 2136
BspEI TCCGGA 2 cut(s) 2115, 2857
BspLI GGNNCC 2 cut(s) 271, 2062
BspMAI CTGCAG 1 cut(s) 2578
BspPI GGATC 4 cut(s) 48, 384, 2126, 2335
BspQI GCTCTTC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 1600
BsrDI GCAATG 3 cut(s) 1099, 1325, 2551
BsrI ACTGG 7 cut(s) 93, 178, 652, 879, 2081, 2542, 2926
BssECI CCNNGG 5 cut(s) 57, 265, 273, 1152, 1718
BssNAI GTATAC 1 cut(s) 705
BssNI GRCGYC 2 cut(s) 45, 2076
BssT1I CCWWGG 3 cut(s) 265, 273, 1152
Bst1107I GTATAC 1 cut(s) 705
Bst2UI CCWGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
Bst4CI ACNGT 8 cut(s) 480, 694, 1104, 1528, 1726, 2219, 2617, 2801
Bst6I CTCTTC 3 cut(s) 336, 1461, 1603
BstACI GRCGYC 2 cut(s) 45, 2076
BstAPI GCANNNNNTGC 2 cut(s) 140, 2396
BstC8I GCNNGC 3 cut(s) 1835, 2392, 2709
BstDEI CTNAG 8 cut(s) 383, 406, 469, 903, 1373, 1713, 2323, 2634
BstF5I GGATG 7 cut(s) 134, 175, 1056, 1148, 1303, 1651, 2866
BstH2I RGCGCY 2 cut(s) 2582, 2669
BstHHI GCGC 3 cut(s) 899, 2581, 2668
BstMAI GTCTC 5 cut(s) 155, 336, 1600, 2115, 2564
BstMWI GCNNNNNNNGC 9 cut(s) 21, 140, 905, 2096, 2396, 2510, 2704, 2938, 2988
BstNI CCWGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
BstNSI RCATGY 5 cut(s) 187, 209, 1837, 1882, 2467
BstSCI CCNGG 6 cut(s) 1330, 1538, 1717, 1795, 1869, 3021
BstSFI CTRYAG 2 cut(s) 537, 2574
BstV1I GCAGC 9 cut(s) 33, 354, 727, 911, 1555, 1689, 2348, 2588, 2944
BstV2I GAAGAC 5 cut(s) 48, 962, 2011, 2150, 2181
BstX2I RGATCY 5 cut(s) 53, 376, 2051, 2327, 2436
BstYI RGATCY 5 cut(s) 53, 376, 2051, 2327, 2436
BstZ17I GTATAC 1 cut(s) 705
Bsu15I ATCGAT 1 cut(s) 2136
BsuI GTATCC 3 cut(s) 1023, 2513, 2761
BsuRI GGCC 2 cut(s) 2394, 3021
BsuTUI ATCGAT 1 cut(s) 2136
BtsCI GGATG 7 cut(s) 134, 175, 1056, 1148, 1303, 1651, 2866
BtsIMutI CAGTG 6 cut(s) 185, 872, 945, 1952, 2088, 2933
Cac8I GCNNGC 3 cut(s) 1835, 2392, 2709
CaiI CAGNNNCTG 2 cut(s) 2330, 2342
CfoI GCGC 3 cut(s) 899, 2581, 2668
Cfr13I GGNCC 2 cut(s) 270, 2209
ClaI ATCGAT 1 cut(s) 2136
CseI GACGC 2 cut(s) 53, 2065
CsiI ACCWGGT 2 cut(s) 1795, 1869
Csp6I GTAC 2 cut(s) 1034, 1793
CviQI GTAC 2 cut(s) 1034, 1793
DdeI CTNAG 8 cut(s) 383, 406, 469, 903, 1373, 1713, 2323, 2634
DraI TTTAAA 1 cut(s) 891
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
Eam1104I CTCTTC 3 cut(s) 336, 1461, 1603
EarI CTCTTC 3 cut(s) 336, 1461, 1603
Ecl136II GAGCTC 2 cut(s) 15, 2305
Eco130I CCWWGG 3 cut(s) 265, 273, 1152
Eco147I AGGCCT 1 cut(s) 2394
Eco24I GRGCYC 2 cut(s) 17, 2307
Eco31I GGTCTC 1 cut(s) 1600
Eco32I GATATC 1 cut(s) 2134
Eco47I GGWCC 2 cut(s) 270, 2209
Eco47III AGCGCT 2 cut(s) 2580, 2667
Eco53kI GAGCTC 2 cut(s) 15, 2305
Eco57I CTGAAG 6 cut(s) 791, 1140, 1781, 2171, 2175, 2757
Eco88I CYCGRG 2 cut(s) 597, 2651
EcoICRI GAGCTC 2 cut(s) 15, 2305
EcoRI GAATTC 2 cut(s) 1177, 1902
EcoRII CCWGG 6 cut(s) 1330, 1538, 1717, 1795, 1869, 3021
EcoRV GATATC 1 cut(s) 2134
EcoT14I CCWWGG 3 cut(s) 265, 273, 1152
EcoT22I ATGCAT 2 cut(s) 1573, 2275
EcoT38I GRGCYC 2 cut(s) 17, 2307
ErhI CCWWGG 3 cut(s) 265, 273, 1152
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 5 cut(s) 283, 653, 1735, 2063, 2908
FauI CCCGC 1 cut(s) 2819
FauNDI CATATG 4 cut(s) 284, 592, 2403, 2587
FbaI TGATCA 1 cut(s) 489
FblI GTMKAC 3 cut(s) 704, 802, 1866
FokI GGATG 7 cut(s) 121, 182, 1043, 1135, 1310, 1638, 2873
FriOI GRGCYC 2 cut(s) 17, 2307
FspBI CTAG 9 cut(s) 224, 248, 951, 1217, 1254, 1428, 1928, 2888, 3014
FspI TGCGCA 1 cut(s) 898
GlaI GCGC 3 cut(s) 898, 2580, 2667
GsaI CCCAGC 1 cut(s) 1672
GsuI CTGGAG 3 cut(s) 1281, 1353, 1539
HaeII RGCGCY 2 cut(s) 2582, 2669
HaeIII GGCC 2 cut(s) 2394, 3021
HapII CCGG 4 cut(s) 2064, 2116, 2567, 2858
HgaI GACGC 2 cut(s) 53, 2065
HhaI GCGC 3 cut(s) 899, 2581, 2668
Hin1I GRCGYC 2 cut(s) 45, 2076
Hin6I GCGC 3 cut(s) 897, 2579, 2666
HinP1I GCGC 3 cut(s) 897, 2579, 2666
HindIII AAGCTT 5 cut(s) 95, 629, 1433, 2153, 2222
HpaII CCGG 4 cut(s) 2064, 2116, 2567, 2858
HphI GGTGA 5 cut(s) 113, 827, 850, 1738, 1845
Hpy166II GTNNAC 7 cut(s) 296, 705, 803, 1745, 1867, 1876, 2477
Hpy8I GTNNAC 7 cut(s) 296, 705, 803, 1745, 1867, 1876, 2477
HpyAV CCTTC 8 cut(s) 656, 1084, 1643, 1937, 2200, 2650, 2866, 2896
HpyCH4III ACNGT 8 cut(s) 480, 694, 1104, 1528, 1726, 2219, 2617, 2801
HpyCH4IV ACGT 4 cut(s) 63, 684, 1924, 2779
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 140, 905, 2096, 2396, 2510, 2704, 2938, 2988
HpyF3I CTNAG 8 cut(s) 383, 406, 469, 903, 1373, 1713, 2323, 2634
HpySE526I ACGT 4 cut(s) 63, 684, 1924, 2779
Hsp92I GRCGYC 2 cut(s) 45, 2076
HspAI GCGC 3 cut(s) 897, 2579, 2666
Kpn2I TCCGGA 2 cut(s) 2115, 2857
Ksp22I TGATCA 1 cut(s) 489
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 3 cut(s) 12, 587, 2954
Lsp1109I GCAGC 9 cut(s) 33, 354, 727, 911, 1555, 1689, 2348, 2588, 2944
LweI GCATC 7 cut(s) 143, 556, 919, 1065, 1157, 1781, 2282
MabI ACCWGGT 2 cut(s) 1795, 1869
MaeI CTAG 9 cut(s) 224, 248, 951, 1217, 1254, 1428, 1928, 2888, 3014
MaeII ACGT 4 cut(s) 63, 684, 1924, 2779
MaeIII GTNAC 3 cut(s) 940, 1170, 2780
MfeI CAATTG 1 cut(s) 2351
MflI RGATCY 5 cut(s) 53, 376, 2051, 2327, 2436
MhlI GDGCHC 3 cut(s) 17, 348, 2307
MlyI GAGTC 6 cut(s) 304, 338, 623, 1597, 2117, 2140
MmeI TCCRAC 4 cut(s) 264, 534, 1275, 1602
Mph1103I ATGCAT 2 cut(s) 1573, 2275
MroI TCCGGA 2 cut(s) 2115, 2857
MroXI GAANNNNTTC 3 cut(s) 1785, 1947, 2739
MseI TTAA 9 cut(s) 188, 482, 513, 890, 987, 1106, 1278, 1457, 2640
MslI CAYNNNNRTG 4 cut(s) 938, 1061, 1589, 2268
MspA1I CMGCKG 2 cut(s) 902, 1118
MspI CCGG 4 cut(s) 2064, 2116, 2567, 2858
MspR9I CCNGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
MunI CAATTG 1 cut(s) 2351
Mva1269I GAATGC 2 cut(s) 1573, 2366
MvaI CCWGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
MwoI GCNNNNNNNGC 9 cut(s) 21, 140, 905, 2096, 2396, 2510, 2704, 2938, 2988
NdeI CATATG 4 cut(s) 284, 592, 2403, 2587
NlaIV GGNNCC 2 cut(s) 271, 2062
NmuCI GTSAC 2 cut(s) 940, 2780
NsbI TGCGCA 1 cut(s) 898
NsiI ATGCAT 2 cut(s) 1573, 2275
NspI RCATGY 5 cut(s) 187, 209, 1837, 1882, 2467
PaeI GCATGC 1 cut(s) 1837
PaeR7I CTCGAG 2 cut(s) 597, 2651
PceI AGGCCT 1 cut(s) 2394
PciI ACATGT 3 cut(s) 205, 1878, 2463
PciSI GCTCTTC 1 cut(s) 336
PctI GAATGC 2 cut(s) 1573, 2366
PdmI GAANNNNTTC 3 cut(s) 1785, 1947, 2739
PleI GAGTC 6 cut(s) 304, 337, 622, 1597, 2116, 2140
PpsI GAGTC 6 cut(s) 304, 337, 622, 1597, 2116, 2140
PscI ACATGT 3 cut(s) 205, 1878, 2463
PshBI ATTAAT 2 cut(s) 987, 1457
Psp124BI GAGCTC 2 cut(s) 17, 2307
Psp6I CCWGG 6 cut(s) 1330, 1538, 1717, 1795, 1869, 3021
PspFI CCCAGC 1 cut(s) 1668
PspGI CCWGG 6 cut(s) 1330, 1538, 1717, 1795, 1869, 3021
PspN4I GGNNCC 2 cut(s) 271, 2062
PspPI GGNCC 2 cut(s) 270, 2209
PspXI VCTCGAGB 1 cut(s) 597
PstI CTGCAG 1 cut(s) 2578
PstNI CAGNNNCTG 2 cut(s) 2330, 2342
PsuI RGATCY 5 cut(s) 53, 376, 2051, 2327, 2436
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 2 cut(s) 1035, 1794
RsaNI GTAC 2 cut(s) 1034, 1793
RseI CAYNNNNRTG 4 cut(s) 938, 1061, 1589, 2268
SacI GAGCTC 2 cut(s) 17, 2307
SapI GCTCTTC 1 cut(s) 336
SaqAI TTAA 9 cut(s) 188, 482, 513, 890, 987, 1106, 1278, 1457, 2640
Sau96I GGNCC 2 cut(s) 270, 2209
SchI GAGTC 6 cut(s) 304, 338, 623, 1597, 2117, 2140
ScrFI CCNGG 6 cut(s) 1332, 1540, 1719, 1797, 1871, 3023
SduI GDGCHC 3 cut(s) 17, 348, 2307
SexAI ACCWGGT 2 cut(s) 1795, 1869
SfaNI GCATC 7 cut(s) 143, 556, 919, 1065, 1157, 1781, 2282
SfcI CTRYAG 2 cut(s) 537, 2574
Sfr274I CTCGAG 2 cut(s) 597, 2651
SinI GGWCC 2 cut(s) 270, 2209
SlaI CTCGAG 2 cut(s) 597, 2651
SmiMI CAYNNNNRTG 4 cut(s) 938, 1061, 1589, 2268
SmlI CTYRAG 7 cut(s) 16, 333, 597, 632, 1185, 1637, 2651
SmoI CTYRAG 7 cut(s) 16, 333, 597, 632, 1185, 1637, 2651
SpeI ACTAGT 1 cut(s) 3013
SphI GCATGC 1 cut(s) 1837
SseBI AGGCCT 1 cut(s) 2394
SsiI CCGC 4 cut(s) 1113, 1116, 1705, 2826
SspMI CTAG 9 cut(s) 224, 248, 951, 1217, 1254, 1428, 1928, 2888, 3014
SstI GAGCTC 2 cut(s) 17, 2307
StuI AGGCCT 1 cut(s) 2394
StyD4I CCNGG 6 cut(s) 1330, 1538, 1717, 1795, 1869, 3021
StyI CCWWGG 3 cut(s) 265, 273, 1152
TaaI ACNGT 8 cut(s) 480, 694, 1104, 1528, 1726, 2219, 2617, 2801
TaiI ACGT 4 cut(s) 66, 687, 1927, 2782
TaqI TCGA 6 cut(s) 320, 598, 1601, 2136, 2652, 2796
TauI GCSGC 1 cut(s) 1118
Tru1I TTAA 9 cut(s) 188, 482, 513, 890, 987, 1106, 1278, 1457, 2640
Tru9I TTAA 9 cut(s) 188, 482, 513, 890, 987, 1106, 1278, 1457, 2640
TscAI CASTG 6 cut(s) 185, 879, 945, 1959, 2088, 2933
TseFI GTSAC 2 cut(s) 940, 2780
TseI GCWGC 9 cut(s) 21, 367, 740, 899, 1543, 1702, 2336, 2576, 2932
Tsp45I GTSAC 2 cut(s) 940, 2780
TspGWI ACGGA 2 cut(s) 1005, 2828
TspRI CASTG 6 cut(s) 185, 879, 945, 1959, 2088, 2933
VpaK11BI GGWCC 2 cut(s) 270, 2209
VspI ATTAAT 2 cut(s) 987, 1457
XapI RAATTY 9 cut(s) 464, 1080, 1177, 1274, 1385, 1902, 1957, 2029, 3003
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 5 cut(s) 187, 209, 1837, 1882, 2467
XhoI CTCGAG 2 cut(s) 597, 2651
XmiI GTMKAC 3 cut(s) 704, 802, 1866
XmnI GAANNNNTTC 3 cut(s) 1785, 1947, 2739
XspI CTAG 9 cut(s) 224, 248, 951, 1217, 1254, 1428, 1928, 2888, 3014
Zsp2I ATGCAT 2 cut(s) 1573, 2275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.