RchiOBHm_Chr4g0396881

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006689
Physical Location & Seq
Reverse (-)
13016616 .. 13023785
7170 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36925

Sequence Viewer

Length: 3015 bp
ATGGAGGGAGGGAGCTCAGAATTGCAGAGCATACAAGACGCCATCAGATCCTCAGATGTGGTGGAGAATCGGATTGAATTACTTAAGAAGCTTGGCGATTTGAAAATAACAAAGAAGTCTGAATTGGCTTCCCTTGCCGACTCGATATTCTGGGAGGAGTATACTTGCTTGGATTTAAGCCAGTGCATGTTAAATAGCGCTATATTACAGGTGGCTGCGAAGCACCTAGAATCAGACACATCCAACTGTCTAGCACATTTCCTTGTCCTTGGGACCAAGGCAAGCATATGGTGTGGAAAGCACCTAAAAATGACTCTTATGTCAACCGAGGAATCTCAAGAAGAAGAGCACGAGAACCTGTTCTTTCAGCTGTTTCTGGATCTGCTGAGCTTTTTGAGGGCTTGCTTTTCAGCTTTGGCAAGATACCCTGTTTCTGTTGAGAAGATGACAGTGGATGTAGTTGAAACATTCTTTGATGAAGAGTTAAACTTGATCAAAGATTCTATATCAGAGATAAAGAGAATTCAGTCATTTGTATCAGAAGAAGTAGTAGTAAAGGTCACACAGGAGGTCATTGATTCTGTAATAAGACTGTGTGGAGCATATGCCCGAGCTTTGAATTGGGAGTCTTGGGAAGAAAAACTCGAGAGGGATAAAACTGACATGGACTTTGAAGGGGCCAATAGTATGAATCATGTTATTAATGTAACAAAGTGTACAATAGAAAAACTATGTGAGATGGGCTTTATTGCAGCTAATAATGGCGGAAGTCTGGTACCGATTATTAATATGTCGTGGAAAGGTGTGGTCACTTTGCTTCAGCTCGGTGATGGGGTATTAGCAACAAAGGTGAATGTAGCAGCTCTAATATCAAATCTAATCTCATTGGTTAATGAATCTATAAGACATGCAGCTGAGGCATGGTTTTCATCACTGAAGGAAAACATCTCTGTGTCAGAAGCTAGAAAGGCATTTCTTCCTGTAAAGTTTTATTTGGTTAATGCAGTAAAAATATCTTCCCTATACCCATATCAAGCATATCTGGTGCAAAGTGAGATAACAAAATGTATCCTGATGATGTCAACCTTAAAGATTTCATTGAGCAATGAAAAACTGTTAAAAACTGCCAGTGATGTTTTCACAGAACTCTTGGAGAAGACATCCTTGGATTTGCTTATTGCTCTACTGAACTCAGTTCAAGTGAAGCGGGAGCTCAAGGTTGAGATTCTAGACTATCTCTTCGGTGAAGGAAGCTGCACAAATTCTGTTGCTGGAGATCCGACTACATGTGGGGAAGCATTGCCCGGAGAAAGGACCTTCTTGATTGGCCGTGTTTCATTGTTTCTTAATTTTCTGAGATTTTCTCTCGATCTTGACGACGATGTAAAGCTTGGGATTGCTGGAAAGCTTGGTTGGTTCTTTGATATGTTAGTAAATGAAGAGGTATATTCTTCCATTCTTCTTTTGCAAGTTCCTGTGCTATATGGTTCTGGAAAAACTGTGGAAGTTGTCCGGCAGCCTTTGTTTTCTTCTCTTTTGAATGCATTGCAAATCTACATGCTTGTGGTTTCTTCTGGTCCTGCTTGGAGGGAGTTGGAGTCTTTTCTTCTTGAGAATTTATTTCACCCCCACTTTCTTTGCTGGGAGATTGTAATGGAACTTTGGTGCTTTATGGTTCGCCATGCTGAGTCTGGGATGGTGAGTGGCATTGCTGGTAAACTTTGCTCATTAATGAAGTTGGTGGCATCTACTGAATCTGTTCTTGTAGCTGATTCTGCTCTGAGAAAAGTGGCAAGGTCAATCAGCATAATTCTTACTTTTGGTGCACAATCTATGGTAGACCAGGTTTACATGGCCATTGTACGTGATGAGGGAACCCAATTGTCATCAGTTATGCGTCTGGCGTTGTTCATGGAGGGCTTCCCACTGAATTTACTGTCAGACAAGATGAAAAGAATTGTGACGCAAAGGATAATTACTGATTACTGTCTCTTCATTGAAAATTTTGATGAGAAGTCAATGAAATCTCTTGATTGTGGTCTTTTTGGGGCCCCAGTTTTTGCTTTGTCAGCTTCGTTGCAGTCTCTTCAGATCAGCACATCTGATATCAATGTGAAGACCTTGAAGTTTTTAGTTGCCATCATTCATAACTACAGAGTTTGTGAAGATCAGCTAATGAAGGACCAGTACCGTAAGCTTCTGAGTGAAACATTAGTGATCATCTCAAATATGAAGCACCTATATGCATCTCTTGAAATGGAGAAAGTAATCTGTGAGCTTGAAAACCTGTTTATCTCAGGACCTGCTGTTTCTGATACCCAATTGTATGAATGCAAACCAGATCTGGCTCTTTTCATGGCAGGCCTTGCTCACATGGATATCAAAGAAGCTGATGAAAGTTCAAAGATCTCTGCTCTATGGGATTTGTATCACATGTTATTGGGAGAGCGGCACTGGGCATTTATTCATCTGGCAATTACAGCATTTGGATACTTTTCTGCTCGGACAAATTGCAATAAGCTCTGGAGATTCGTGCCTCAGAATGCAGCGCTTTCCTATGATCTGACTTCTGGAAAGGAGGCAAATCTGGAGCGATTTATGTCTGAGTTCAAGGTTTTTCTTGAGAAGGAAACGGCACTTCTCACAATAACGGCTAGCTCTGACCAGCTTGGTCTGCTTGTGAGGGAGGGTCTAAAACTAAAAGAAATGTTCCAAAAGATTTCAAATGTCGTCGTTGAGACTGCGGAATGTGACTACATGGAAATTGATGGTGAAAAGCAGGCCATGGAGATGAACGGTGGAAAACAATCCAATAGGAAAAGGAAATTTCCTGATGGAATTGAAAAGGGAATGGAACTGCTACAAAGTGGTATGAAGGTTATTGTGGATAGTATTTCTCAGTTGCAGCAAAATGATCAACCCAGTTCCAATGAACTACCCGACAACTTCTTGACCACCTTTTCTCGCCTTGAAGCTGAAATTTCTCAACTAGTTGGCCTGGCTGGTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

1004

Amino Acids

112.49

Weight (kDa)

5.02

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 261 - 841 1.7e-149 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
Acc36I ACCTGC 1 cut(s) 2311
Acc65I GGTACC 1 cut(s) 775
AccB1I GGYRCC 1 cut(s) 775
AccBSI CCGCTC 1 cut(s) 2449
AccI GTMKAC 2 cut(s) 161, 1839
AciI CCGC 4 cut(s) 765, 1207, 2449, 2744
AclWI GGATC 3 cut(s) 42, 387, 1271
AcoI YGGCCR 2 cut(s) 1327, 1854
AcsI RAATTY 7 cut(s) 522, 1261, 1615, 1930, 2002, 2825, 2979
AcuI CTGAAG 3 cut(s) 803, 956, 2072
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 4 cut(s) 718, 777, 1863, 2189
AfeI AGCGCT 2 cut(s) 199, 2550
AfiI CCNNNNNNNGG 1 cut(s) 1311
AflII CTTAAG 1 cut(s) 83
AflIII ACRYGT 2 cut(s) 1286, 2433
AhlI ACTAGT 1 cut(s) 2989
AjnI CCWGG 2 cut(s) 1842, 2997
AjuI GAANNNNNNNTTGG 6 cut(s) 107, 139, 1148, 1180, 1396, 1428
Alw21I GWGCWC 4 cut(s) 17, 351, 1215, 1828
Alw26I GTCTC 3 cut(s) 1994, 2088, 2732
Alw44I GTGCAC 1 cut(s) 1824
AlwI GGATC 3 cut(s) 42, 387, 1271
AlwNI CAGNNNCTG 1 cut(s) 2303
Ama87I CYCGRG 2 cut(s) 609, 644
Aor51HI AGCGCT 2 cut(s) 199, 2550
AoxI GGCC 7 cut(s) 678, 1327, 1854, 2049, 2362, 2781, 2995
ApaI GGGCCC 1 cut(s) 2053
ApaLI GTGCAC 1 cut(s) 1824
ApeKI GCWGC 8 cut(s) 215, 752, 860, 911, 1254, 1516, 2546, 2905
ApoI RAATTY 7 cut(s) 522, 1261, 1615, 1930, 2002, 2825, 2979
ArsI GACNNNNNNTTYG 4 cut(s) 653, 685, 1223, 1255
AseI ATTAAT 3 cut(s) 702, 786, 1730
Asp700I GAANNNNTTC 2 cut(s) 359, 1758
Asp718I GGTACC 1 cut(s) 775
AspLEI GCGC 2 cut(s) 200, 2551
AspS9I GGNCC 8 cut(s) 273, 678, 1314, 1577, 2049, 2050, 2182, 2300
AsuC2I CCSGG 1 cut(s) 1305
AsuHPI GGTGA 6 cut(s) 839, 862, 1256, 1616, 1711, 2783
AsuNHI GCTAGC 1 cut(s) 2654
AvaI CYCGRG 2 cut(s) 609, 644
AvaII GGWCC 5 cut(s) 273, 1314, 1577, 2182, 2300
BaeGI GKGCMC 2 cut(s) 1828, 2053
BalI TGGCCA 1 cut(s) 1856
BanI GGYRCC 1 cut(s) 775
BanII GRGCYC 3 cut(s) 17, 1215, 2053
BarI GAAGNNNNNNTAC 2 cut(s) 2171, 2203
BauI CACGAG 1 cut(s) 350
BbsI GAAGAC 2 cut(s) 1163, 2123
Bbv12I GWGCWC 4 cut(s) 17, 351, 1215, 1828
BbvCI CCTCAGC 1 cut(s) 915
BbvI GCAGC 8 cut(s) 202, 764, 872, 923, 1241, 1528, 2558, 2917
BccI CCATC 7 cut(s) 50, 733, 824, 1690, 2147, 2762, 2828
BceAI ACGGC 3 cut(s) 1314, 2649, 2667
BciT130I CCWGG 2 cut(s) 1844, 2999
BciVI GTATCC 2 cut(s) 1079, 2483
BclI TGATCA 3 cut(s) 492, 2217, 2914
BcnI CCSGG 1 cut(s) 1305
BcoDI GTCTC 3 cut(s) 1994, 2088, 2732
BcuI ACTAGT 1 cut(s) 2989
BfaI CTAG 6 cut(s) 227, 251, 963, 1229, 2655, 2990
BfmI CTRYAG 1 cut(s) 2152
BfoI RGCGCY 2 cut(s) 201, 2552
BfrI CTTAAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 2311
BfuI GTATCC 2 cut(s) 1079, 2483
BglII AGATCT 2 cut(s) 2341, 2406
BisI GCNGC 9 cut(s) 216, 753, 861, 912, 1255, 1517, 2450, 2547, 2906
BlpI GCTNAGC 1 cut(s) 386
BlsI GCNGC 9 cut(s) 217, 754, 862, 913, 1256, 1518, 2451, 2548, 2907
Bme1390I CCNGG 3 cut(s) 1305, 1844, 2999
Bme18I GGWCC 5 cut(s) 273, 1314, 1577, 2182, 2300
BmeT110I CYCGRG 2 cut(s) 609, 644
BmgT120I GGNCC 8 cut(s) 273, 678, 1314, 1577, 2049, 2050, 2182, 2300
BmiI GGNNCC 7 cut(s) 274, 679, 777, 1876, 2050, 2051, 2052
BmrFI CCNGG 3 cut(s) 1305, 1844, 2999
BmrI ACTGGG 3 cut(s) 2048, 2464, 2916
BmsI GCATC 2 cut(s) 1754, 2255
BmtI GCTAGC 1 cut(s) 2658
BmuI ACTGGG 3 cut(s) 2048, 2464, 2916
BpiI GAAGAC 2 cut(s) 1163, 2123
BplI GAGNNNNNCTC 2 cut(s) 1348, 1380
BpmI CTGGAG 3 cut(s) 1293, 2545, 2609
Bpu10I CCTNAGC 1 cut(s) 915
Bpu1102I GCTNAGC 1 cut(s) 386
BpuEI CTTGAG 4 cut(s) 321, 1199, 1631, 2642
BpuMI CCSGG 1 cut(s) 1305
BsaAI YACGTR 1 cut(s) 1865
BsaBI GATNNNNATC 1 cut(s) 2427
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 5 cut(s) 268, 276, 327, 1164, 2784
BsaXI ACNNNNNCTCC 2 cut(s) 1583, 1613
Bsc4I CCNNNNNNNGG 1 cut(s) 1311
Bse1I ACTGG 6 cut(s) 181, 1128, 2054, 2185, 2459, 2922
Bse3DI GCAATG 4 cut(s) 1111, 1298, 1544, 1707
Bse8I GATNNNNATC 1 cut(s) 2427
BseBI CCWGG 2 cut(s) 1844, 2999
BseDI CCNNGG 5 cut(s) 268, 276, 327, 1164, 2784
BseGI GGATG 4 cut(s) 239, 460, 1160, 1701
BseJI GATNNNNATC 1 cut(s) 2427
BseLI CCNNNNNNNGG 1 cut(s) 1311
BseMI GCAATG 4 cut(s) 1111, 1298, 1544, 1707
BseNI ACTGG 6 cut(s) 181, 1128, 2054, 2185, 2459, 2922
BseRI GAGGAG 1 cut(s) 170
BseSI GKGCMC 2 cut(s) 1828, 2053
BseXI GCAGC 8 cut(s) 202, 764, 872, 923, 1241, 1528, 2558, 2917
BseYI CCCAGC 1 cut(s) 1641
BsgI GTGCAG 1 cut(s) 1240
BshFI GGCC 7 cut(s) 680, 1329, 1856, 2051, 2364, 2783, 2997
BshNI GGYRCC 1 cut(s) 775
BsiHKAI GWGCWC 4 cut(s) 17, 351, 1215, 1828
BsiHKCI CYCGRG 2 cut(s) 609, 644
BsiSI CCGG 2 cut(s) 1305, 1513
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 1311
BsmAI GTCTC 3 cut(s) 1994, 2088, 2732
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 3 cut(s) 1546, 2336, 2548
BsnI GGCC 7 cut(s) 680, 1329, 1856, 2051, 2364, 2783, 2997
BsoBI CYCGRG 2 cut(s) 609, 644
Bsp120I GGGCCC 1 cut(s) 2049
Bsp1286I GDGCHC 5 cut(s) 17, 351, 1215, 1828, 2053
Bsp1407I TGTACA 1 cut(s) 716
Bsp1720I GCTNAGC 1 cut(s) 386
Bsp19I CCATGG 1 cut(s) 2784
BspACI CCGC 4 cut(s) 765, 1207, 2449, 2744
BspANI GGCC 7 cut(s) 680, 1329, 1856, 2051, 2364, 2783, 2997
BspLI GGNNCC 7 cut(s) 274, 679, 777, 1876, 2050, 2051, 2052
BspMI ACCTGC 1 cut(s) 2311
BspOI GCTAGC 1 cut(s) 2658
BspPI GGATC 3 cut(s) 42, 387, 1271
BspQI GCTCTTC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 775
BspTI CTTAAG 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 2449
BsrDI GCAATG 4 cut(s) 1111, 1298, 1544, 1707
BsrGI TGTACA 1 cut(s) 716
BsrI ACTGG 6 cut(s) 181, 1128, 2054, 2185, 2459, 2922
BssECI CCNNGG 5 cut(s) 268, 276, 327, 1164, 2784
BssNAI GTATAC 1 cut(s) 162
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 350
BssT1I CCWWGG 4 cut(s) 268, 276, 1164, 2784
Bst1107I GTATAC 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 350
Bst2UI CCWGG 2 cut(s) 1844, 2999
Bst4CI ACNGT 9 cut(s) 248, 451, 594, 1116, 1501, 1938, 1988, 2192, 2798
Bst6I CTCTTC 6 cut(s) 339, 474, 1244, 1434, 1997, 2091
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 2366
BstAUI TGTACA 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 1865
BstC8I GCNNGC 5 cut(s) 283, 403, 2362, 2656, 2781
BstDSI CCRYGG 1 cut(s) 2784
BstF5I GGATG 4 cut(s) 239, 460, 1160, 1701
BstH2I RGCGCY 2 cut(s) 201, 2552
BstHHI GCGC 2 cut(s) 200, 2551
BstMAI GTCTC 3 cut(s) 1994, 2088, 2732
BstMWI GCNNNNNNNGC 8 cut(s) 134, 917, 968, 1775, 2069, 2366, 2480, 2674
BstNI CCWGG 2 cut(s) 1844, 2999
BstNSI RCATGY 5 cut(s) 190, 911, 1290, 1561, 2437
BstSCI CCNGG 3 cut(s) 1303, 1842, 2997
BstSFI CTRYAG 1 cut(s) 2152
BstSLI GKGCMC 2 cut(s) 1828, 2053
BstV1I GCAGC 8 cut(s) 202, 764, 872, 923, 1241, 1528, 2558, 2917
BstV2I GAAGAC 2 cut(s) 1163, 2123
BstX2I RGATCY 5 cut(s) 47, 379, 1276, 2341, 2406
BstYI RGATCY 5 cut(s) 47, 379, 1276, 2341, 2406
BstZ17I GTATAC 1 cut(s) 162
BsuI GTATCC 2 cut(s) 1079, 2483
BsuRI GGCC 7 cut(s) 680, 1329, 1856, 2051, 2364, 2783, 2997
BtgI CCRYGG 1 cut(s) 2784
BtsCI GGATG 4 cut(s) 239, 460, 1160, 1701
BtsIMutI CAGTG 6 cut(s) 188, 456, 932, 1135, 1925, 2452
BveI ACCTGC 1 cut(s) 2311
Cac8I GCNNGC 5 cut(s) 283, 403, 2362, 2656, 2781
CaiI CAGNNNCTG 1 cut(s) 2303
CfoI GCGC 2 cut(s) 200, 2551
Cfr13I GGNCC 8 cut(s) 273, 678, 1314, 1577, 2049, 2050, 2182, 2300
CseI GACGC 3 cut(s) 47, 1886, 1972
CsiI ACCWGGT 1 cut(s) 1842
Csp6I GTAC 4 cut(s) 717, 776, 1862, 2188
CspCI CAANNNNNGTGG 2 cut(s) 1619, 1654
CviQI GTAC 4 cut(s) 717, 776, 1862, 2188
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 2 cut(s) 1327, 1854
Eam1104I CTCTTC 6 cut(s) 339, 474, 1244, 1434, 1997, 2091
EarI CTCTTC 6 cut(s) 339, 474, 1244, 1434, 1997, 2091
EciI GGCGGA 1 cut(s) 780
Ecl136II GAGCTC 2 cut(s) 15, 1213
Eco130I CCWWGG 4 cut(s) 268, 276, 1164, 2784
Eco147I AGGCCT 1 cut(s) 2364
Eco24I GRGCYC 3 cut(s) 17, 1215, 2053
Eco32I GATATC 2 cut(s) 2107, 2380
Eco47I GGWCC 5 cut(s) 273, 1314, 1577, 2182, 2300
Eco47III AGCGCT 2 cut(s) 199, 2550
Eco53kI GAGCTC 2 cut(s) 15, 1213
Eco57I CTGAAG 3 cut(s) 803, 956, 2072
Eco88I CYCGRG 2 cut(s) 609, 644
EcoICRI GAGCTC 2 cut(s) 15, 1213
EcoO109I RGGNCCY 4 cut(s) 1314, 2049, 2050, 2300
EcoRI GAATTC 1 cut(s) 522
EcoRII CCWGG 2 cut(s) 1842, 2997
EcoRV GATATC 2 cut(s) 2107, 2380
EcoT14I CCWWGG 4 cut(s) 268, 276, 1164, 2784
EcoT22I ATGCAT 2 cut(s) 1546, 2248
EcoT38I GRGCYC 3 cut(s) 17, 1215, 2053
ErhI CCWWGG 4 cut(s) 268, 276, 1164, 2784
FalI AAGNNNNNCTT 2 cut(s) 1148, 1180
FaqI GGGAC 1 cut(s) 286
FauI CCCGC 1 cut(s) 1200
FauNDI CATATG 2 cut(s) 287, 604
FbaI TGATCA 3 cut(s) 492, 2217, 2914
FblI GTMKAC 2 cut(s) 161, 1839
Fnu4HI GCNGC 9 cut(s) 216, 753, 861, 912, 1255, 1517, 2450, 2547, 2906
FokI GGATG 4 cut(s) 226, 467, 1147, 1708
FriOI GRGCYC 3 cut(s) 17, 1215, 2053
Fsp4HI GCNGC 9 cut(s) 216, 753, 861, 912, 1255, 1517, 2450, 2547, 2906
FspBI CTAG 6 cut(s) 227, 251, 963, 1229, 2655, 2990
GlaI GCGC 2 cut(s) 199, 2550
GluI GCNGC 9 cut(s) 216, 753, 861, 912, 1255, 1517, 2450, 2547, 2906
GsaI CCCAGC 1 cut(s) 1645
GsuI CTGGAG 3 cut(s) 1293, 2545, 2609
HaeII RGCGCY 2 cut(s) 201, 2552
HaeIII GGCC 7 cut(s) 680, 1329, 1856, 2051, 2364, 2783, 2997
HapII CCGG 2 cut(s) 1305, 1513
HgaI GACGC 3 cut(s) 47, 1886, 1972
HhaI GCGC 2 cut(s) 200, 2551
Hin1I GRCGYC 1 cut(s) 39
Hin6I GCGC 2 cut(s) 198, 2549
HinP1I GCGC 2 cut(s) 198, 2549
HincII GTYRAC 2 cut(s) 324, 1083
HindII GTYRAC 2 cut(s) 324, 1083
HindIII AAGCTT 4 cut(s) 89, 1388, 1406, 2195
HpaII CCGG 2 cut(s) 1305, 1513
HphI GGTGA 6 cut(s) 839, 862, 1256, 1616, 1711, 2783
Hpy166II GTNNAC 8 cut(s) 162, 324, 717, 1083, 1718, 1826, 1840, 1849
Hpy8I GTNNAC 8 cut(s) 162, 324, 717, 1083, 1718, 1826, 1840, 1849
Hpy99I CGWCG 2 cut(s) 1382, 2735
HpyAV CCTTC 7 cut(s) 668, 931, 1241, 1327, 2173, 2620, 2869
HpyCH4III ACNGT 9 cut(s) 248, 451, 594, 1116, 1501, 1938, 1988, 2192, 2798
HpyCH4IV ACGT 1 cut(s) 1864
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 917, 968, 1775, 2069, 2366, 2480, 2674
HpySE526I ACGT 1 cut(s) 1864
Hsp92I GRCGYC 1 cut(s) 39
HspAI GCGC 2 cut(s) 198, 2549
KpnI GGTACC 1 cut(s) 779
Ksp22I TGATCA 3 cut(s) 492, 2217, 2914
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 4 cut(s) 12, 599, 1210, 2590
Lsp1109I GCAGC 8 cut(s) 202, 764, 872, 923, 1241, 1528, 2558, 2917
LweI GCATC 2 cut(s) 1754, 2255
MabI ACCWGGT 1 cut(s) 1842
MaeI CTAG 6 cut(s) 227, 251, 963, 1229, 2655, 2990
MaeII ACGT 1 cut(s) 1864
MaeIII GTNAC 5 cut(s) 559, 706, 808, 1960, 2750
MbiI CCGCTC 1 cut(s) 2449
MfeI CAATTG 2 cut(s) 1880, 2321
MflI RGATCY 5 cut(s) 47, 379, 1276, 2341, 2406
MhlI GDGCHC 5 cut(s) 17, 351, 1215, 1828, 2053
MlsI TGGCCA 1 cut(s) 1856
MluNI TGGCCA 1 cut(s) 1856
MlyI GAGTC 5 cut(s) 134, 307, 635, 1607, 1697
MmeI TCCRAC 3 cut(s) 267, 1304, 1575
Mox20I TGGCCA 1 cut(s) 1856
Mph1103I ATGCAT 2 cut(s) 1546, 2248
MroXI GAANNNNTTC 2 cut(s) 359, 1758
MscI TGGCCA 1 cut(s) 1856
MslI CAYNNNNRTG 4 cut(s) 950, 1562, 2241, 2789
Msp20I TGGCCA 1 cut(s) 1856
MspA1I CMGCKG 2 cut(s) 370, 914
MspCI CTTAAG 1 cut(s) 83
MspI CCGG 2 cut(s) 1305, 1513
MspR9I CCNGG 3 cut(s) 1305, 1844, 2999
MunI CAATTG 2 cut(s) 1880, 2321
Mva1269I GAATGC 3 cut(s) 1546, 2336, 2548
MvaI CCWGG 2 cut(s) 1844, 2999
MwoI GCNNNNNNNGC 8 cut(s) 134, 917, 968, 1775, 2069, 2366, 2480, 2674
NciI CCSGG 1 cut(s) 1305
NcoI CCATGG 1 cut(s) 2784
NdeI CATATG 2 cut(s) 287, 604
NheI GCTAGC 1 cut(s) 2654
NlaIV GGNNCC 7 cut(s) 274, 679, 777, 1876, 2050, 2051, 2052
NmuCI GTSAC 4 cut(s) 559, 808, 1960, 2750
NsiI ATGCAT 2 cut(s) 1546, 2248
NspI RCATGY 5 cut(s) 190, 911, 1290, 1561, 2437
PaeR7I CTCGAG 1 cut(s) 644
PceI AGGCCT 1 cut(s) 2364
PciI ACATGT 2 cut(s) 1286, 2433
PciSI GCTCTTC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 1374
PctI GAATGC 3 cut(s) 1546, 2336, 2548
PdmI GAANNNNTTC 2 cut(s) 359, 1758
PkrI GCNGC 9 cut(s) 217, 754, 862, 913, 1256, 1518, 2451, 2548, 2907
PleI GAGTC 5 cut(s) 134, 307, 634, 1606, 1696
PpsI GAGTC 5 cut(s) 134, 307, 634, 1606, 1696
Ppu21I YACGTR 1 cut(s) 1865
PpuMI RGGWCCY 2 cut(s) 1314, 2300
PscI ACATGT 2 cut(s) 1286, 2433
PshBI ATTAAT 3 cut(s) 702, 786, 1730
Psp124BI GAGCTC 2 cut(s) 17, 1215
Psp5II RGGWCCY 2 cut(s) 1314, 2300
Psp6I CCWGG 2 cut(s) 1842, 2997
PspFI CCCAGC 1 cut(s) 1641
PspGI CCWGG 2 cut(s) 1842, 2997
PspN4I GGNNCC 7 cut(s) 274, 679, 777, 1876, 2050, 2051, 2052
PspOMI GGGCCC 1 cut(s) 2049
PspPI GGNCC 8 cut(s) 273, 678, 1314, 1577, 2049, 2050, 2182, 2300
PspPPI RGGWCCY 2 cut(s) 1314, 2300
PstNI CAGNNNCTG 1 cut(s) 2303
PsuI RGATCY 5 cut(s) 47, 379, 1276, 2341, 2406
PvuII CAGCTG 2 cut(s) 370, 914
RsaI GTAC 4 cut(s) 718, 777, 1863, 2189
RsaNI GTAC 4 cut(s) 717, 776, 1862, 2188
RseI CAYNNNNRTG 4 cut(s) 950, 1562, 2241, 2789
SacI GAGCTC 2 cut(s) 17, 1215
SapI GCTCTTC 1 cut(s) 339
SatI GCNGC 9 cut(s) 216, 753, 861, 912, 1255, 1517, 2450, 2547, 2906
Sau96I GGNCC 8 cut(s) 273, 678, 1314, 1577, 2049, 2050, 2182, 2300
SchI GAGTC 5 cut(s) 134, 307, 635, 1607, 1697
ScrFI CCNGG 3 cut(s) 1305, 1844, 2999
SduI GDGCHC 5 cut(s) 17, 351, 1215, 1828, 2053
SexAI ACCWGGT 1 cut(s) 1842
SfaNI GCATC 2 cut(s) 1754, 2255
SfcI CTRYAG 1 cut(s) 2152
Sfr274I CTCGAG 1 cut(s) 644
SinI GGWCC 5 cut(s) 273, 1314, 1577, 2182, 2300
SlaI CTCGAG 1 cut(s) 644
SmiMI CAYNNNNRTG 4 cut(s) 950, 1562, 2241, 2789
SmlI CTYRAG 6 cut(s) 83, 336, 644, 1214, 1610, 2621
SmoI CTYRAG 6 cut(s) 83, 336, 644, 1214, 1610, 2621
SpeI ACTAGT 1 cut(s) 2989
SseBI AGGCCT 1 cut(s) 2364
SsiI CCGC 4 cut(s) 765, 1207, 2449, 2744
SspMI CTAG 6 cut(s) 227, 251, 963, 1229, 2655, 2990
SstI GAGCTC 2 cut(s) 17, 1215
StuI AGGCCT 1 cut(s) 2364
StyD4I CCNGG 3 cut(s) 1303, 1842, 2997
StyI CCWWGG 4 cut(s) 268, 276, 1164, 2784
TaaI ACNGT 9 cut(s) 248, 451, 594, 1116, 1501, 1938, 1988, 2192, 2798
TaiI ACGT 1 cut(s) 1867
TaqI TCGA 3 cut(s) 143, 645, 1368
TatI WGTACW 1 cut(s) 716
TauI GCSGC 1 cut(s) 2452
TscAI CASTG 6 cut(s) 188, 456, 939, 1135, 1932, 2459
TseFI GTSAC 4 cut(s) 559, 808, 1960, 2750
TseI GCWGC 8 cut(s) 215, 752, 860, 911, 1254, 1516, 2546, 2905
Tsp45I GTSAC 4 cut(s) 559, 808, 1960, 2750
TspRI CASTG 6 cut(s) 188, 456, 939, 1135, 1932, 2459
Vha464I CTTAAG 1 cut(s) 83
VneI GTGCAC 1 cut(s) 1824
VpaK11BI GGWCC 5 cut(s) 273, 1314, 1577, 2182, 2300
VspI ATTAAT 3 cut(s) 702, 786, 1730
XapI RAATTY 7 cut(s) 522, 1261, 1615, 1930, 2002, 2825, 2979
XbaI TCTAGA 1 cut(s) 1228
XceI RCATGY 5 cut(s) 190, 911, 1290, 1561, 2437
XcmI CCANNNNNNNNNTGG 1 cut(s) 1688
XhoI CTCGAG 1 cut(s) 644
XmiI GTMKAC 2 cut(s) 161, 1839
XmnI GAANNNNTTC 2 cut(s) 359, 1758
XspI CTAG 6 cut(s) 227, 251, 963, 1229, 2655, 2990
Zsp2I ATGCAT 2 cut(s) 1546, 2248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.