Rh4CG079100

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006689
Physical Location & Seq
Reverse (-)
14229085 .. 14235875
6791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG079100.1

Sequence Viewer

Length: 2829 bp
ATGTTAAATAGCGCTATATTACAGGTGGCTGCGAAGCACCTAGAATCAGACACATCCAACTGTCTAGCACATTTCCTTGTCCTTGGGACCAAGGCAAGCATATGGTGTGGAAAGCACCTAAAAATGACTCTTATGTCAACCGAGGAATCTCAAGAAGAAGAGCACGAGAACCTGTTCTTTCAGCTGTTTCTGGATCTGCTGAGCTTTTTGAGGGCTTGCTTTTCAGCTTTGGCAAGATACCCTGTTTCTGTTGAGAAGATGACAGTGGATGTAGTTGAAACATTCTTTGATGAAGGGTTAAACTTGATCAAAGATTCTATATCAGAGATAAAGAGAATTCAGTCATTTGTATCAGAAGAAGTCGTAGTAAAGGTTACACAGGAGGTCATTGATTCTGTAATAAGATTGTGTGGAGCATATGCCCGAGCTTTGAATTGGGAGTCTTGGGAAGAAAAACTCGAGAGGGATAAAACTGACATGGACTTTGAAGGGGCCAATAGTATGAATCATGTTATTAATGTAACAAAGTGTACAATAGAAAAACTATGTGAGATGGGCATTATTGCAGCTAATAATGGCGGAAGTCTGGTACCGATTATTAATATGTCGTGGAAAGGTGTGGTCACTTTGCTTCAGCTCGGTGATGGGGTATTAGCAACAAAGGTGAATGTAGCAGCTCTAATATCAAATCTAATCTCATTGGTTAATGAATCTATAAGACATGCAGCTGAGGCATGGTTTTCATCACTGAAGGAAAACATCTCTGTGTCAGAAGCTAGAAAGGCATTTCTTCCTATAAAGTTTTATTTGGTTAATGCAGTAAAAATATCTTCCCTATACCCATATCAAGCATATCTGGTGCAAAGTGAGATAACAAAATGTATCCTGATGATGTCAACCTTAAAGATTTCATTGAGCAATGAAAACCTGTTAAAAACTGCCAGTGATGTTTTCACAGAACTCTTGGAGAAGACATCCTTGGATTTGCTTATTGCTCTACTGAACTCAGTTCAAGTGAAGCGGGAGCTCAAGGTTGAGATTCTAGACTATCTCTTCGGTGAAGGAAGCTGCACAAATTCTGTTGATGGAGATCCGACTACATGTGGGGAAGCATTGCCCGGAGAAAGGACCTTCTTGATTGGCCGTGTTTCATTGTTTCTTAATTTTCTGAGATTTTCTCTCGATCTTGACGACGATGTAAAGCTTGGGATTGCTGGAAAGCTTGGTTGGTTCTTTGATATGTTAGTAAATGAAGAGGTATATTCTTCCATTCTTCTTTTGCAAGTTCCTGTGCTATATGGTTCTGGAAAAACTGTGGAAGTTGTCTGGCAACCTTTGTTTTCTTCTCTTTTGAATGCATTGCAAATCTACATGCTTGTCGTTTCTTCTGGTCCTGCTTGGAGGGAGCTGGAGTCTTTTCTTCTTGAGAATTTATTTCACCCCCACTTTCTTTGCTGGGAGATTGTAATGGAACTTTGGTGCTTTATGGTTCGCCATGCTGAGTCTGGGATGGTGAGTGGCATTGCTGGTAAACTTTGCTCATTAATGAAGTTGGTGGCATCTACTGAATCCGTTCTTGTAGCTGATTCTGCTCTGAGAAAAGTGGCAAGGTCAATCAGCATAATTCTTACTTTTGGTGCACAATCTATGGTAGACCAGGTTTACATGGCCATTGTACGTGATGAGGGAACCCAATTGTCATCAGTTATGCGTCTGGCGTTGTTCATGGAGGGCTTCCCACTGAATTTACTGTCAGACAAGATGAAAAGAATTGCGACGCAAAGGATAATTACTGATTACTGTCTCTTCGTTGAAAATTTTGATGAGAAGTCAATGAAATCTCTTGATTGTGGTCTTTTTGGGGCCCCAGTTTTTGCTTTGTCAGCTTCGTTGCAGTCTCTTCAGATCAGCACATCTGATATCAATGTGAAGACCTTGAAGTTTTTAGTTGCCATCATTCATAACTACAGAGTTTGTGAAGATCAGCTAATGAAGGACCAGTACTGTAAGCTTCTGAGTGAAACATTAGTGATCATCTCAAATATGAAGCACCTATATGCATCTCTTGAAATGGAGAAAGTAATCTGTGAGCTTGAAAACCTGTTTATCTCGGGACCTGCTGTTTCTGATACCCAATTGTATGAATGCAAACCAGATCTGGCTCTTTTCATGGCAGGCCTTGCTCACATGGATATCAAAGAAGCTGATGAAAGTTCCAAGATCTCTGCTCTATGGGATTTGTATCACATGTTATTGGGAGAGCGGCACTGGGCATTTATTCATTTGGCAATTACAGCATTTGGATACTTTTCTGCTCGGACAAATTGCAATAAGCTCTGGAGATTCGTGCCTCAGAATGCAGCGCTTTCCTATGATCTGACTTCTGGAAATGAGGCCAATCTGGAGCGATTTATGTCTGAGTTCAAGGTTTTTCTTGAGAAGGAAACGGCACTTCTCACAATAACGGCTAGCTCTGACCAGCTTGGTCTGCTTGTGAGGGAGGGTCTAAAACTAAAAGAAATGTTCCAAAAGATTTCAAATGTCGTCGTTGAGACTGCGGAATGTGACTACATGGAAATTGATGGTGAAAAGCAGGCCATGGAGATGAACGGTGGAAAACAATCCAATAGGAAAAGGAAATTTCCTGATGGAATTGAAAAGGGAATGGAACTGCTACAAAGTGGTATGAAGGTTATTGTGGATAGTATTTCTCAGTTGCAGCAAAATGATCAACTCAGTCCCAATGAACTACCCGACAACTTCTTGACCACCTTTTCTCGCCTTGAAGCTGAAATTTCTCAACTAGTTGGCCTGGCTGGTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

942

Amino Acids

105.46

Weight (kDa)

4.99

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 199 - 779 8.9e-150 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
Acc36I ACCTGC 1 cut(s) 2125
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 2263
AccI GTMKAC 1 cut(s) 1653
AciI CCGC 4 cut(s) 579, 1021, 2263, 2558
AclWI GGATC 2 cut(s) 201, 1085
AcoI YGGCCR 2 cut(s) 1141, 1668
AcsI RAATTY 7 cut(s) 336, 1075, 1429, 1744, 1816, 2639, 2793
AcuI CTGAAG 3 cut(s) 617, 770, 1886
AfaI GTAC 4 cut(s) 532, 591, 1677, 2003
AfeI AGCGCT 2 cut(s) 13, 2364
AfiI CCNNNNNNNGG 1 cut(s) 1125
AflIII ACRYGT 2 cut(s) 1100, 2247
AhlI ACTAGT 1 cut(s) 2803
AjnI CCWGG 2 cut(s) 1656, 2811
AjuI GAANNNNNNNTTGG 4 cut(s) 962, 994, 1210, 1242
Alw21I GWGCWC 3 cut(s) 165, 1029, 1642
Alw26I GTCTC 3 cut(s) 1808, 1902, 2546
Alw44I GTGCAC 1 cut(s) 1638
AlwI GGATC 2 cut(s) 201, 1085
Ama87I CYCGRG 3 cut(s) 423, 458, 2110
Aor51HI AGCGCT 2 cut(s) 13, 2364
AoxI GGCC 8 cut(s) 492, 1141, 1668, 1863, 2176, 2394, 2595, 2809
ApaI GGGCCC 1 cut(s) 1867
ApaLI GTGCAC 1 cut(s) 1638
ApeKI GCWGC 7 cut(s) 29, 566, 674, 725, 1068, 2360, 2719
ApoI RAATTY 7 cut(s) 336, 1075, 1429, 1744, 1816, 2639, 2793
ArsI GACNNNNNNTTYG 4 cut(s) 467, 499, 1037, 1069
AseI ATTAAT 3 cut(s) 516, 600, 1544
Asp700I GAANNNNTTC 2 cut(s) 173, 1572
Asp718I GGTACC 1 cut(s) 589
AspLEI GCGC 2 cut(s) 14, 2365
AspS9I GGNCC 8 cut(s) 87, 492, 1128, 1391, 1863, 1864, 1996, 2114
AsuC2I CCSGG 1 cut(s) 1119
AsuHPI GGTGA 6 cut(s) 653, 676, 1070, 1430, 1525, 2597
AsuNHI GCTAGC 1 cut(s) 2468
AvaI CYCGRG 3 cut(s) 423, 458, 2110
AvaII GGWCC 5 cut(s) 87, 1128, 1391, 1996, 2114
BaeGI GKGCMC 2 cut(s) 1642, 1867
BalI TGGCCA 1 cut(s) 1670
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 1029, 1867
BarI GAAGNNNNNNTAC 2 cut(s) 1985, 2017
BauI CACGAG 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 977, 1937
Bbv12I GWGCWC 3 cut(s) 165, 1029, 1642
BbvCI CCTCAGC 1 cut(s) 729
BbvI GCAGC 7 cut(s) 16, 578, 686, 737, 1055, 2372, 2731
BccI CCATC 7 cut(s) 547, 638, 1079, 1504, 1961, 2576, 2642
BceAI ACGGC 3 cut(s) 1128, 2463, 2481
BciT130I CCWGG 2 cut(s) 1658, 2813
BciVI GTATCC 2 cut(s) 893, 2297
BclI TGATCA 3 cut(s) 306, 2031, 2728
BcnI CCSGG 1 cut(s) 1119
BcoDI GTCTC 3 cut(s) 1808, 1902, 2546
BcuI ACTAGT 1 cut(s) 2803
BfaI CTAG 6 cut(s) 41, 65, 777, 1043, 2469, 2804
BfmI CTRYAG 1 cut(s) 1966
BfoI RGCGCY 2 cut(s) 15, 2366
BfuAI ACCTGC 1 cut(s) 2125
BfuI GTATCC 2 cut(s) 893, 2297
BglII AGATCT 2 cut(s) 2155, 2220
BisI GCNGC 8 cut(s) 30, 567, 675, 726, 1069, 2264, 2361, 2720
BlpI GCTNAGC 1 cut(s) 200
BlsI GCNGC 8 cut(s) 31, 568, 676, 727, 1070, 2265, 2362, 2721
BmcAI AGTACT 1 cut(s) 2003
Bme1390I CCNGG 3 cut(s) 1119, 1658, 2813
Bme18I GGWCC 5 cut(s) 87, 1128, 1391, 1996, 2114
BmeT110I CYCGRG 3 cut(s) 423, 458, 2110
BmgT120I GGNCC 8 cut(s) 87, 492, 1128, 1391, 1863, 1864, 1996, 2114
BmiI GGNNCC 8 cut(s) 88, 493, 591, 1690, 1864, 1865, 1866, 2115
BmrFI CCNGG 3 cut(s) 1119, 1658, 2813
BmrI ACTGGG 2 cut(s) 1862, 2278
BmsI GCATC 2 cut(s) 1568, 2069
BmtI GCTAGC 1 cut(s) 2472
BmuI ACTGGG 2 cut(s) 1862, 2278
BpiI GAAGAC 2 cut(s) 977, 1937
BplI GAGNNNNNCTC 2 cut(s) 1162, 1194
BpmI CTGGAG 3 cut(s) 1430, 2359, 2423
Bpu10I CCTNAGC 1 cut(s) 729
Bpu1102I GCTNAGC 1 cut(s) 200
BpuEI CTTGAG 4 cut(s) 135, 1013, 1445, 2456
BpuMI CCSGG 1 cut(s) 1119
BsaAI YACGTR 1 cut(s) 1679
BsaBI GATNNNNATC 2 cut(s) 1089, 2241
BsaJI CCNNGG 5 cut(s) 82, 90, 141, 978, 2598
BsaXI ACNNNNNCTCC 2 cut(s) 1397, 1427
Bsc4I CCNNNNNNNGG 1 cut(s) 1125
Bse1I ACTGG 4 cut(s) 942, 1868, 1999, 2273
Bse3DI GCAATG 4 cut(s) 925, 1112, 1358, 1521
Bse8I GATNNNNATC 2 cut(s) 1089, 2241
BseBI CCWGG 2 cut(s) 1658, 2813
BseDI CCNNGG 5 cut(s) 82, 90, 141, 978, 2598
BseGI GGATG 4 cut(s) 53, 274, 974, 1515
BseJI GATNNNNATC 2 cut(s) 1089, 2241
BseLI CCNNNNNNNGG 1 cut(s) 1125
BseMI GCAATG 4 cut(s) 925, 1112, 1358, 1521
BseNI ACTGG 4 cut(s) 942, 1868, 1999, 2273
BseSI GKGCMC 2 cut(s) 1642, 1867
BseXI GCAGC 7 cut(s) 16, 578, 686, 737, 1055, 2372, 2731
BseYI CCCAGC 1 cut(s) 1455
BsgI GTGCAG 1 cut(s) 1054
BshFI GGCC 8 cut(s) 494, 1143, 1670, 1865, 2178, 2396, 2597, 2811
BshNI GGYRCC 1 cut(s) 589
BsiHKAI GWGCWC 3 cut(s) 165, 1029, 1642
BsiHKCI CYCGRG 3 cut(s) 423, 458, 2110
BsiSI CCGG 1 cut(s) 1119
BslFI GGGAC 3 cut(s) 100, 2127, 2724
BslI CCNNNNNNNGG 1 cut(s) 1125
BsmAI GTCTC 3 cut(s) 1808, 1902, 2546
BsmFI GGGAC 3 cut(s) 100, 2127, 2724
BsmI GAATGC 3 cut(s) 1360, 2150, 2362
BsnI GGCC 8 cut(s) 494, 1143, 1670, 1865, 2178, 2396, 2597, 2811
BsoBI CYCGRG 3 cut(s) 423, 458, 2110
Bsp120I GGGCCC 1 cut(s) 1863
Bsp1286I GDGCHC 4 cut(s) 165, 1029, 1642, 1867
Bsp1407I TGTACA 1 cut(s) 530
Bsp1720I GCTNAGC 1 cut(s) 200
Bsp19I CCATGG 1 cut(s) 2598
BspACI CCGC 4 cut(s) 579, 1021, 2263, 2558
BspANI GGCC 8 cut(s) 494, 1143, 1670, 1865, 2178, 2396, 2597, 2811
BspLI GGNNCC 8 cut(s) 88, 493, 591, 1690, 1864, 1865, 1866, 2115
BspMI ACCTGC 1 cut(s) 2125
BspOI GCTAGC 1 cut(s) 2472
BspPI GGATC 2 cut(s) 201, 1085
BspQI GCTCTTC 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 589
BsrBI CCGCTC 1 cut(s) 2263
BsrDI GCAATG 4 cut(s) 925, 1112, 1358, 1521
BsrGI TGTACA 1 cut(s) 530
BsrI ACTGG 4 cut(s) 942, 1868, 1999, 2273
BssECI CCNNGG 5 cut(s) 82, 90, 141, 978, 2598
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 4 cut(s) 82, 90, 978, 2598
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 1658, 2813
Bst4CI ACNGT 7 cut(s) 62, 265, 1315, 1752, 1802, 2006, 2612
Bst6I CTCTTC 5 cut(s) 153, 1058, 1248, 1811, 1905
BstAPI GCANNNNNTGC 1 cut(s) 2180
BstAUI TGTACA 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 1679
BstC8I GCNNGC 5 cut(s) 97, 217, 2176, 2470, 2595
BstDSI CCRYGG 1 cut(s) 2598
BstF5I GGATG 4 cut(s) 53, 274, 974, 1515
BstH2I RGCGCY 2 cut(s) 15, 2366
BstHHI GCGC 2 cut(s) 14, 2365
BstMAI GTCTC 3 cut(s) 1808, 1902, 2546
BstMWI GCNNNNNNNGC 7 cut(s) 731, 782, 1589, 1883, 2180, 2294, 2488
BstNI CCWGG 2 cut(s) 1658, 2813
BstNSI RCATGY 4 cut(s) 725, 1104, 1375, 2251
BstSCI CCNGG 3 cut(s) 1117, 1656, 2811
BstSFI CTRYAG 1 cut(s) 1966
BstSLI GKGCMC 2 cut(s) 1642, 1867
BstV1I GCAGC 7 cut(s) 16, 578, 686, 737, 1055, 2372, 2731
BstV2I GAAGAC 2 cut(s) 977, 1937
BstX2I RGATCY 4 cut(s) 193, 1090, 2155, 2220
BstYI RGATCY 4 cut(s) 193, 1090, 2155, 2220
BsuI GTATCC 2 cut(s) 893, 2297
BsuRI GGCC 8 cut(s) 494, 1143, 1670, 1865, 2178, 2396, 2597, 2811
BtgI CCRYGG 1 cut(s) 2598
BtsCI GGATG 4 cut(s) 53, 274, 974, 1515
BtsIMutI CAGTG 5 cut(s) 270, 746, 949, 1739, 2266
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 5 cut(s) 97, 217, 2176, 2470, 2595
CfoI GCGC 2 cut(s) 14, 2365
Cfr13I GGNCC 8 cut(s) 87, 492, 1128, 1391, 1863, 1864, 1996, 2114
CseI GACGC 2 cut(s) 1700, 1786
CsiI ACCWGGT 1 cut(s) 1656
Csp6I GTAC 4 cut(s) 531, 590, 1676, 2002
CspCI CAANNNNNGTGG 2 cut(s) 1433, 1468
CviQI GTAC 4 cut(s) 531, 590, 1676, 2002
DrdI GACNNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 1141, 1668
Eam1104I CTCTTC 5 cut(s) 153, 1058, 1248, 1811, 1905
EarI CTCTTC 5 cut(s) 153, 1058, 1248, 1811, 1905
EciI GGCGGA 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 4 cut(s) 82, 90, 978, 2598
Eco147I AGGCCT 1 cut(s) 2178
Eco24I GRGCYC 2 cut(s) 1029, 1867
Eco32I GATATC 2 cut(s) 1921, 2194
Eco47I GGWCC 5 cut(s) 87, 1128, 1391, 1996, 2114
Eco47III AGCGCT 2 cut(s) 13, 2364
Eco53kI GAGCTC 1 cut(s) 1027
Eco57I CTGAAG 3 cut(s) 617, 770, 1886
Eco88I CYCGRG 3 cut(s) 423, 458, 2110
EcoICRI GAGCTC 1 cut(s) 1027
EcoO109I RGGNCCY 4 cut(s) 1128, 1863, 1864, 2114
EcoRI GAATTC 1 cut(s) 336
EcoRII CCWGG 2 cut(s) 1656, 2811
EcoRV GATATC 2 cut(s) 1921, 2194
EcoT14I CCWWGG 4 cut(s) 82, 90, 978, 2598
EcoT22I ATGCAT 2 cut(s) 1360, 2062
EcoT38I GRGCYC 2 cut(s) 1029, 1867
ErhI CCWWGG 4 cut(s) 82, 90, 978, 2598
FalI AAGNNNNNCTT 2 cut(s) 962, 994
FaqI GGGAC 3 cut(s) 100, 2127, 2724
FauI CCCGC 1 cut(s) 1014
FauNDI CATATG 2 cut(s) 101, 418
FbaI TGATCA 3 cut(s) 306, 2031, 2728
FblI GTMKAC 1 cut(s) 1653
Fnu4HI GCNGC 8 cut(s) 30, 567, 675, 726, 1069, 2264, 2361, 2720
FokI GGATG 4 cut(s) 40, 281, 961, 1522
FriOI GRGCYC 2 cut(s) 1029, 1867
Fsp4HI GCNGC 8 cut(s) 30, 567, 675, 726, 1069, 2264, 2361, 2720
FspBI CTAG 6 cut(s) 41, 65, 777, 1043, 2469, 2804
GlaI GCGC 2 cut(s) 13, 2364
GluI GCNGC 8 cut(s) 30, 567, 675, 726, 1069, 2264, 2361, 2720
GsaI CCCAGC 1 cut(s) 1459
GsuI CTGGAG 3 cut(s) 1430, 2359, 2423
HaeII RGCGCY 2 cut(s) 15, 2366
HaeIII GGCC 8 cut(s) 494, 1143, 1670, 1865, 2178, 2396, 2597, 2811
HapII CCGG 1 cut(s) 1119
HgaI GACGC 2 cut(s) 1700, 1786
HhaI GCGC 2 cut(s) 14, 2365
Hin6I GCGC 2 cut(s) 12, 2363
HinP1I GCGC 2 cut(s) 12, 2363
HincII GTYRAC 2 cut(s) 138, 897
HindII GTYRAC 2 cut(s) 138, 897
HindIII AAGCTT 3 cut(s) 1202, 1220, 2009
HpaII CCGG 1 cut(s) 1119
HphI GGTGA 6 cut(s) 653, 676, 1070, 1430, 1525, 2597
Hpy166II GTNNAC 7 cut(s) 138, 531, 897, 1532, 1640, 1654, 1663
Hpy8I GTNNAC 7 cut(s) 138, 531, 897, 1532, 1640, 1654, 1663
Hpy99I CGWCG 3 cut(s) 1196, 1780, 2549
HpyAV CCTTC 8 cut(s) 287, 482, 745, 1055, 1141, 1987, 2434, 2683
HpyCH4III ACNGT 7 cut(s) 62, 265, 1315, 1752, 1802, 2006, 2612
HpyCH4IV ACGT 1 cut(s) 1678
HpyF10VI GCNNNNNNNGC 7 cut(s) 731, 782, 1589, 1883, 2180, 2294, 2488
HpySE526I ACGT 1 cut(s) 1678
HspAI GCGC 2 cut(s) 12, 2363
KpnI GGTACC 1 cut(s) 593
Ksp22I TGATCA 3 cut(s) 306, 2031, 2728
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 4 cut(s) 413, 1024, 1405, 2404
Lsp1109I GCAGC 7 cut(s) 16, 578, 686, 737, 1055, 2372, 2731
LweI GCATC 2 cut(s) 1568, 2069
MabI ACCWGGT 1 cut(s) 1656
MaeI CTAG 6 cut(s) 41, 65, 777, 1043, 2469, 2804
MaeII ACGT 1 cut(s) 1678
MaeIII GTNAC 4 cut(s) 373, 520, 622, 2564
MbiI CCGCTC 1 cut(s) 2263
MfeI CAATTG 2 cut(s) 1694, 2135
MflI RGATCY 4 cut(s) 193, 1090, 2155, 2220
MhlI GDGCHC 4 cut(s) 165, 1029, 1642, 1867
MlsI TGGCCA 1 cut(s) 1670
MluNI TGGCCA 1 cut(s) 1670
MlyI GAGTC 4 cut(s) 121, 449, 1421, 1511
MmeI TCCRAC 2 cut(s) 81, 1118
Mox20I TGGCCA 1 cut(s) 1670
Mph1103I ATGCAT 2 cut(s) 1360, 2062
MroXI GAANNNNTTC 2 cut(s) 173, 1572
MscI TGGCCA 1 cut(s) 1670
MslI CAYNNNNRTG 3 cut(s) 764, 2055, 2603
Msp20I TGGCCA 1 cut(s) 1670
MspA1I CMGCKG 2 cut(s) 184, 728
MspI CCGG 1 cut(s) 1119
MspR9I CCNGG 3 cut(s) 1119, 1658, 2813
MunI CAATTG 2 cut(s) 1694, 2135
Mva1269I GAATGC 3 cut(s) 1360, 2150, 2362
MvaI CCWGG 2 cut(s) 1658, 2813
MwoI GCNNNNNNNGC 7 cut(s) 731, 782, 1589, 1883, 2180, 2294, 2488
NciI CCSGG 1 cut(s) 1119
NcoI CCATGG 1 cut(s) 2598
NdeI CATATG 2 cut(s) 101, 418
NheI GCTAGC 1 cut(s) 2468
NlaIV GGNNCC 8 cut(s) 88, 493, 591, 1690, 1864, 1865, 1866, 2115
NmuCI GTSAC 2 cut(s) 622, 2564
NsiI ATGCAT 2 cut(s) 1360, 2062
NspI RCATGY 4 cut(s) 725, 1104, 1375, 2251
PaeR7I CTCGAG 1 cut(s) 458
PceI AGGCCT 1 cut(s) 2178
PciI ACATGT 2 cut(s) 1100, 2247
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 1188
PctI GAATGC 3 cut(s) 1360, 2150, 2362
PdmI GAANNNNTTC 2 cut(s) 173, 1572
PkrI GCNGC 8 cut(s) 31, 568, 676, 727, 1070, 2265, 2362, 2721
PleI GAGTC 4 cut(s) 121, 448, 1420, 1510
PpsI GAGTC 4 cut(s) 121, 448, 1420, 1510
Ppu21I YACGTR 1 cut(s) 1679
PpuMI RGGWCCY 2 cut(s) 1128, 2114
PscI ACATGT 2 cut(s) 1100, 2247
PshBI ATTAAT 3 cut(s) 516, 600, 1544
Psp124BI GAGCTC 1 cut(s) 1029
Psp5II RGGWCCY 2 cut(s) 1128, 2114
Psp6I CCWGG 2 cut(s) 1656, 2811
PspFI CCCAGC 1 cut(s) 1455
PspGI CCWGG 2 cut(s) 1656, 2811
PspN4I GGNNCC 8 cut(s) 88, 493, 591, 1690, 1864, 1865, 1866, 2115
PspOMI GGGCCC 1 cut(s) 1863
PspPI GGNCC 8 cut(s) 87, 492, 1128, 1391, 1863, 1864, 1996, 2114
PspPPI RGGWCCY 2 cut(s) 1128, 2114
PsuI RGATCY 4 cut(s) 193, 1090, 2155, 2220
PvuII CAGCTG 2 cut(s) 184, 728
RsaI GTAC 4 cut(s) 532, 591, 1677, 2003
RsaNI GTAC 4 cut(s) 531, 590, 1676, 2002
RseI CAYNNNNRTG 3 cut(s) 764, 2055, 2603
SacI GAGCTC 1 cut(s) 1029
SapI GCTCTTC 1 cut(s) 153
SatI GCNGC 8 cut(s) 30, 567, 675, 726, 1069, 2264, 2361, 2720
Sau96I GGNCC 8 cut(s) 87, 492, 1128, 1391, 1863, 1864, 1996, 2114
ScaI AGTACT 1 cut(s) 2003
SchI GAGTC 4 cut(s) 121, 449, 1421, 1511
ScrFI CCNGG 3 cut(s) 1119, 1658, 2813
SduI GDGCHC 4 cut(s) 165, 1029, 1642, 1867
SexAI ACCWGGT 1 cut(s) 1656
SfaNI GCATC 2 cut(s) 1568, 2069
SfcI CTRYAG 1 cut(s) 1966
Sfr274I CTCGAG 1 cut(s) 458
SinI GGWCC 5 cut(s) 87, 1128, 1391, 1996, 2114
SlaI CTCGAG 1 cut(s) 458
SmiMI CAYNNNNRTG 3 cut(s) 764, 2055, 2603
SmlI CTYRAG 5 cut(s) 150, 458, 1028, 1424, 2435
SmoI CTYRAG 5 cut(s) 150, 458, 1028, 1424, 2435
SpeI ACTAGT 1 cut(s) 2803
SseBI AGGCCT 1 cut(s) 2178
SsiI CCGC 4 cut(s) 579, 1021, 2263, 2558
SspMI CTAG 6 cut(s) 41, 65, 777, 1043, 2469, 2804
SstI GAGCTC 1 cut(s) 1029
StuI AGGCCT 1 cut(s) 2178
StyD4I CCNGG 3 cut(s) 1117, 1656, 2811
StyI CCWWGG 4 cut(s) 82, 90, 978, 2598
TaaI ACNGT 7 cut(s) 62, 265, 1315, 1752, 1802, 2006, 2612
TaiI ACGT 1 cut(s) 1681
TaqI TCGA 2 cut(s) 459, 1182
TatI WGTACW 2 cut(s) 530, 2001
TauI GCSGC 1 cut(s) 2266
TscAI CASTG 5 cut(s) 270, 753, 949, 1746, 2273
TseFI GTSAC 2 cut(s) 622, 2564
TseI GCWGC 7 cut(s) 29, 566, 674, 725, 1068, 2360, 2719
Tsp45I GTSAC 2 cut(s) 622, 2564
TspGWI ACGGA 1 cut(s) 1561
TspRI CASTG 5 cut(s) 270, 753, 949, 1746, 2273
VneI GTGCAC 1 cut(s) 1638
VpaK11BI GGWCC 5 cut(s) 87, 1128, 1391, 1996, 2114
VspI ATTAAT 3 cut(s) 516, 600, 1544
XapI RAATTY 7 cut(s) 336, 1075, 1429, 1744, 1816, 2639, 2793
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 4 cut(s) 725, 1104, 1375, 2251
XcmI CCANNNNNNNNNTGG 1 cut(s) 1502
XhoI CTCGAG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 1653
XmnI GAANNNNTTC 2 cut(s) 173, 1572
XspI CTAG 6 cut(s) 41, 65, 777, 1043, 2469, 2804
ZrmI AGTACT 1 cut(s) 2003
Zsp2I ATGCAT 2 cut(s) 1360, 2062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.