Rh4BG071000

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006689
Physical Location & Seq
Reverse (-)
12574744 .. 12581521
6778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG071000.1

Sequence Viewer

Length: 2958 bp
ATGCAGGTGGTGGAGAATCGGATTGAATTACTTAAGAAGCTTGGCGATTTGAAAATAACAGAGAAGTCTGAATTGGCTTCCCTTGCCGAGTGTTTGACAGAGTATACTTGCTTGGATTTAAGCCAGTGCATGTTAAATAGCGCTATATTACAGGTGGCTGCGAAGCACCTAGAATCAGACACATCCAACTGTCTAGCACATTTCCTTGTCCTTGGGACCAAGGCAAGCATATGGTGTGGAAAGCACCTAAAAATGACTCTTATGTCAACCGAGGAATCTCAAGAAGAAGAGCACGAGAACCTGTTCTTTCAGCTGTTTCTGGATCTGCTGAGCTTTTTGAGGGCTTGCTTTTCAGCTTTGGCAAGATACCCTGTTTCTGTTGAGAAGATGACAGTGGATGTAGTTGAAACATTCTTTGATGAAGGGTTAAACTTGATCAAAGATTCTATATCAGAGATAAAGAGAATTCAGTCATTTGTATCAGAAGAAGTCGTAGTAAAGGTTACACAGGAGGTCATTGATTCTGTAATAAGATTGTGTGGAGCATATGCCCGAGCTTTGAATTGGGAGTCTTGGGAAGAAAAACTCGAGAGGGATAAAACTGACATGGACTTTGAAGGGGCCAATAGTATGAATCATGTTATTAATGTAACAAAGTGTACAATAGAAAAACTATGTGAGATGGGCATTATTGCAGCTAATAATGGCGGAAGTCTGGTACCGATTATTAATATGTCGTGGAAAGGTGTGGTCACTTTGCTTCAGCTCGGTGATGGGGTATTAGCAACAAAGGTGAATGTAGCAGCTCTAATATCAAATCTAATCTCATTGGTTAATGAATCTATAAGACATGCAGCTGAGGCATGGTTTTCATCACTGAAGGAAAACATCTCTGTGTCAGAAGCTAGAAAGGCATTTCTTCCTATAAAGTTTTATTTGGTTAATGCAGTAAAAATATCTTCCCTATACCCATATCAAGCATATCTGGTGCAAAGTGAGATAACAAAATGTATCCTGATGATGTCAACCTTAAAGATTTCATTGAGCAATGAAAACCTGTTAAAAACTGCCAGTGATGTTTTCACAGAACTCTTGGAGAAGACATCCTTGGATTTGCTTATTGCTCTACTGAACTCAGTTCAAGTGAAGCGGGAGCTCAAGGTTGAGATTCTAGACTATCTCTTCGGTGAAGGAAGCTGCACAAATTCTGTTGATGGAGATCCGACTACATGTGGGGAAGCATTGCCCGGAGAAAGGACCTTCTTGATTGGCCGTGTTTCATTGTTTCTTAATTTTCTGAGATTTTCTCTCGATCTTGACGACGATGTAAAGCTTGGGATTGCTGGAAAGCTTGGTTGGTTCTTTGATATGTTAGTAAATGAAGAGGTATATTCTTCCATTCTTCTTTTGCAAGTTCCTGTGCTATATGGTTCTGGAAAAACTGTGGAAGTTGTCTGGCAACCTTTGTTTTCTTCTCTTTTGAATGCATTGCAAATCTACATGCTTGTCGTTTCTTCTGGTCCTGCTTGGAGGGAGCTGGAGTCTTTTCTTCTTGAGAATTTATTTCACCCCCACTTTCTTTGCTGGGAGATTGTAATGGAACTTTGGTGCTTTATGGTTCGCCATGCTGAGTCTGGGATGGTGAGTGGCATTGCTGGTAAACTTTGCTCATTAATGAAGTTGGTGGCATCTACTGAATCCGTTCTTGTAGCTGATTCTGCTCTGAGAAAAGTGGCAAGGTCAATCAGCATAATTCTTACTTTTGGTGCACAATCTATGGTAGACCAGGTTTACATGGCCATTGTACGTGATGAGGGAACCCAATTGTCATCAGTTATGCGTCTGGCGTTGTTCATGGAGGGCTTCCCACTGAATTTACTGTCAGACAAGATGAAAAGAATTGCGACGCAAAGGATAATTACTGATTACTGTCTCTTCGTTGAAAATTTTGATGAGAAGTCAATGAAATCTCTTGATTGTGGTCTTTTTGGGGCCCCAGTTTTTGCTTTGTCAGCTTCGTTGCAGTCTCTTCAGATCAGCACATCTGATATCAATGTGAAGACCTTGAAGTTTTTAGTTGCCATCATTCATAACTACAGAGTTTGTGAAGATCAGCTAATGAAGGACCAGTACTGTAAGCTTCTGAGTGAAACATTAGTGATCATCTCAAATATGAAGCACCTATATGCATCTCTTGAAATGGAGAAAGTAATCTGTGAGCTTGAAAACCTGTTTATCTCGGGACCTGCTGTTTCTGATACCCAATTGTATGAATGCAAACCAGATCTGGCTCTTTTCATGGCAGGCCTTGCTCACATGGATATCAAAGAAGCTGATGAAAGTTCCAAGATCTCTGCTCTATGGGATTTGTATCACATGTTATTGGGAGAGCGGCACTGGGCATTTATTCATTTGGCAATTACAGCATTTGGATACTTTTCTGCTCGGACAAATTGCAATAAGCTCTGGAGATTCGTGCCTCAGAATGCAGCGCTTTCCTATGATCTGACTTCTGGAAATGAGGCCAATCTGGAGCGATTTATGTCTGAGTTCAAGGTTTTTCTTGAGAAGGAAACGGCACTTCTCACAATAACGGCTAGCTCTGACCAGCTTGGTCTGCTTGTGAGGGAGGGTCTAAAACTAAAAGAAATGTTCCAAAAGATTTCAAATGTCGTCGTTGAGACTGCGGAATGTGACTACATGGAAATTGATGGTGAAAAGCAGGCCATGGAGATGAACGGTGGAAAACAATCCAATAGGAAAAGGAAATTTCCTGATGGAATTGAAAAGGGAATGGAACTGCTACAAAGTGGTATGAAGGTTATTGTGGATAGTATTTCTCAGTTGCAGCAAAATGATCAACTCAGTCCCAATGAACTACCCGACAACTTCTTGACCACCTTTTCTCGCCTTGAAGCTGAAATTTCTCAACTAGTTGGCCTGGCTGGTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

985

Amino Acids

110.31

Weight (kDa)

4.96

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 242 - 822 1e-149 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 2254
Acc65I GGTACC 1 cut(s) 718
AccB1I GGYRCC 1 cut(s) 718
AccBSI CCGCTC 1 cut(s) 2392
AccI GTMKAC 2 cut(s) 104, 1782
AciI CCGC 4 cut(s) 708, 1150, 2392, 2687
AclWI GGATC 2 cut(s) 330, 1214
AcoI YGGCCR 2 cut(s) 1270, 1797
AcsI RAATTY 7 cut(s) 465, 1204, 1558, 1873, 1945, 2768, 2922
AcuI CTGAAG 3 cut(s) 746, 899, 2015
AfaI GTAC 4 cut(s) 661, 720, 1806, 2132
AfeI AGCGCT 2 cut(s) 142, 2493
AfiI CCNNNNNNNGG 1 cut(s) 1254
AflII CTTAAG 1 cut(s) 32
AflIII ACRYGT 2 cut(s) 1229, 2376
AhlI ACTAGT 1 cut(s) 2932
AjnI CCWGG 2 cut(s) 1785, 2940
AjuI GAANNNNNNNTTGG 6 cut(s) 56, 88, 1091, 1123, 1339, 1371
Alw21I GWGCWC 3 cut(s) 294, 1158, 1771
Alw26I GTCTC 3 cut(s) 1937, 2031, 2675
Alw44I GTGCAC 1 cut(s) 1767
AlwI GGATC 2 cut(s) 330, 1214
Ama87I CYCGRG 3 cut(s) 552, 587, 2239
Aor51HI AGCGCT 2 cut(s) 142, 2493
AoxI GGCC 8 cut(s) 621, 1270, 1797, 1992, 2305, 2523, 2724, 2938
ApaI GGGCCC 1 cut(s) 1996
ApaLI GTGCAC 1 cut(s) 1767
ApeKI GCWGC 7 cut(s) 158, 695, 803, 854, 1197, 2489, 2848
ApoI RAATTY 7 cut(s) 465, 1204, 1558, 1873, 1945, 2768, 2922
ArsI GACNNNNNNTTYG 4 cut(s) 596, 628, 1166, 1198
AseI ATTAAT 3 cut(s) 645, 729, 1673
Asp700I GAANNNNTTC 2 cut(s) 302, 1701
Asp718I GGTACC 1 cut(s) 718
AspLEI GCGC 2 cut(s) 143, 2494
AspS9I GGNCC 8 cut(s) 216, 621, 1257, 1520, 1992, 1993, 2125, 2243
AsuC2I CCSGG 1 cut(s) 1248
AsuHPI GGTGA 6 cut(s) 782, 805, 1199, 1559, 1654, 2726
AsuNHI GCTAGC 1 cut(s) 2597
AvaI CYCGRG 3 cut(s) 552, 587, 2239
AvaII GGWCC 5 cut(s) 216, 1257, 1520, 2125, 2243
BaeGI GKGCMC 2 cut(s) 1771, 1996
BalI TGGCCA 1 cut(s) 1799
BanI GGYRCC 1 cut(s) 718
BanII GRGCYC 2 cut(s) 1158, 1996
BarI GAAGNNNNNNTAC 2 cut(s) 2114, 2146
BauI CACGAG 1 cut(s) 293
BbsI GAAGAC 2 cut(s) 1106, 2066
Bbv12I GWGCWC 3 cut(s) 294, 1158, 1771
BbvCI CCTCAGC 1 cut(s) 858
BbvI GCAGC 7 cut(s) 145, 707, 815, 866, 1184, 2501, 2860
BccI CCATC 7 cut(s) 676, 767, 1208, 1633, 2090, 2705, 2771
BceAI ACGGC 3 cut(s) 1257, 2592, 2610
BciT130I CCWGG 2 cut(s) 1787, 2942
BciVI GTATCC 2 cut(s) 1022, 2426
BclI TGATCA 3 cut(s) 435, 2160, 2857
BcnI CCSGG 1 cut(s) 1248
BcoDI GTCTC 3 cut(s) 1937, 2031, 2675
BcuI ACTAGT 1 cut(s) 2932
BfaI CTAG 6 cut(s) 170, 194, 906, 1172, 2598, 2933
BfmI CTRYAG 1 cut(s) 2095
BfoI RGCGCY 2 cut(s) 144, 2495
BfrI CTTAAG 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 2254
BfuI GTATCC 2 cut(s) 1022, 2426
BglII AGATCT 2 cut(s) 2284, 2349
BisI GCNGC 8 cut(s) 159, 696, 804, 855, 1198, 2393, 2490, 2849
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 8 cut(s) 160, 697, 805, 856, 1199, 2394, 2491, 2850
BmcAI AGTACT 1 cut(s) 2132
Bme1390I CCNGG 3 cut(s) 1248, 1787, 2942
Bme18I GGWCC 5 cut(s) 216, 1257, 1520, 2125, 2243
BmeT110I CYCGRG 3 cut(s) 552, 587, 2239
BmgT120I GGNCC 8 cut(s) 216, 621, 1257, 1520, 1992, 1993, 2125, 2243
BmiI GGNNCC 8 cut(s) 217, 622, 720, 1819, 1993, 1994, 1995, 2244
BmrFI CCNGG 3 cut(s) 1248, 1787, 2942
BmrI ACTGGG 2 cut(s) 1991, 2407
BmsI GCATC 2 cut(s) 1697, 2198
BmtI GCTAGC 1 cut(s) 2601
BmuI ACTGGG 2 cut(s) 1991, 2407
BpiI GAAGAC 2 cut(s) 1106, 2066
BplI GAGNNNNNCTC 2 cut(s) 1291, 1323
BpmI CTGGAG 3 cut(s) 1559, 2488, 2552
Bpu10I CCTNAGC 1 cut(s) 858
Bpu1102I GCTNAGC 1 cut(s) 329
BpuEI CTTGAG 4 cut(s) 264, 1142, 1574, 2585
BpuMI CCSGG 1 cut(s) 1248
BsaAI YACGTR 1 cut(s) 1808
BsaBI GATNNNNATC 2 cut(s) 1218, 2370
BsaJI CCNNGG 5 cut(s) 211, 219, 270, 1107, 2727
BsaXI ACNNNNNCTCC 2 cut(s) 1526, 1556
Bsc4I CCNNNNNNNGG 1 cut(s) 1254
Bse1I ACTGG 5 cut(s) 124, 1071, 1997, 2128, 2402
Bse3DI GCAATG 4 cut(s) 1054, 1241, 1487, 1650
Bse8I GATNNNNATC 2 cut(s) 1218, 2370
BseBI CCWGG 2 cut(s) 1787, 2942
BseDI CCNNGG 5 cut(s) 211, 219, 270, 1107, 2727
BseGI GGATG 4 cut(s) 182, 403, 1103, 1644
BseJI GATNNNNATC 2 cut(s) 1218, 2370
BseLI CCNNNNNNNGG 1 cut(s) 1254
BseMI GCAATG 4 cut(s) 1054, 1241, 1487, 1650
BseNI ACTGG 5 cut(s) 124, 1071, 1997, 2128, 2402
BseSI GKGCMC 2 cut(s) 1771, 1996
BseXI GCAGC 7 cut(s) 145, 707, 815, 866, 1184, 2501, 2860
BseYI CCCAGC 1 cut(s) 1584
BsgI GTGCAG 1 cut(s) 1183
BshFI GGCC 8 cut(s) 623, 1272, 1799, 1994, 2307, 2525, 2726, 2940
BshNI GGYRCC 1 cut(s) 718
BsiHKAI GWGCWC 3 cut(s) 294, 1158, 1771
BsiHKCI CYCGRG 3 cut(s) 552, 587, 2239
BsiSI CCGG 1 cut(s) 1248
BslFI GGGAC 3 cut(s) 229, 2256, 2853
BslI CCNNNNNNNGG 1 cut(s) 1254
BsmAI GTCTC 3 cut(s) 1937, 2031, 2675
BsmFI GGGAC 3 cut(s) 229, 2256, 2853
BsmI GAATGC 3 cut(s) 1489, 2279, 2491
BsnI GGCC 8 cut(s) 623, 1272, 1799, 1994, 2307, 2525, 2726, 2940
BsoBI CYCGRG 3 cut(s) 552, 587, 2239
Bsp120I GGGCCC 1 cut(s) 1992
Bsp1286I GDGCHC 4 cut(s) 294, 1158, 1771, 1996
Bsp1407I TGTACA 1 cut(s) 659
Bsp1720I GCTNAGC 1 cut(s) 329
Bsp19I CCATGG 1 cut(s) 2727
BspACI CCGC 4 cut(s) 708, 1150, 2392, 2687
BspANI GGCC 8 cut(s) 623, 1272, 1799, 1994, 2307, 2525, 2726, 2940
BspLI GGNNCC 8 cut(s) 217, 622, 720, 1819, 1993, 1994, 1995, 2244
BspMI ACCTGC 1 cut(s) 2254
BspOI GCTAGC 1 cut(s) 2601
BspPI GGATC 2 cut(s) 330, 1214
BspQI GCTCTTC 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 718
BspTI CTTAAG 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 2392
BsrDI GCAATG 4 cut(s) 1054, 1241, 1487, 1650
BsrGI TGTACA 1 cut(s) 659
BsrI ACTGG 5 cut(s) 124, 1071, 1997, 2128, 2402
BssECI CCNNGG 5 cut(s) 211, 219, 270, 1107, 2727
BssNAI GTATAC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 4 cut(s) 211, 219, 1107, 2727
Bst1107I GTATAC 1 cut(s) 105
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 2 cut(s) 1787, 2942
Bst4CI ACNGT 7 cut(s) 191, 394, 1444, 1881, 1931, 2135, 2741
Bst6I CTCTTC 5 cut(s) 282, 1187, 1377, 1940, 2034
BstAFI CTTAAG 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 2309
BstAUI TGTACA 1 cut(s) 659
BstBAI YACGTR 1 cut(s) 1808
BstC8I GCNNGC 5 cut(s) 226, 346, 2305, 2599, 2724
BstDSI CCRYGG 1 cut(s) 2727
BstF5I GGATG 4 cut(s) 182, 403, 1103, 1644
BstH2I RGCGCY 2 cut(s) 144, 2495
BstHHI GCGC 2 cut(s) 143, 2494
BstMAI GTCTC 3 cut(s) 1937, 2031, 2675
BstMWI GCNNNNNNNGC 8 cut(s) 83, 860, 911, 1718, 2012, 2309, 2423, 2617
BstNI CCWGG 2 cut(s) 1787, 2942
BstNSI RCATGY 5 cut(s) 133, 854, 1233, 1504, 2380
BstSCI CCNGG 3 cut(s) 1246, 1785, 2940
BstSFI CTRYAG 1 cut(s) 2095
BstSLI GKGCMC 2 cut(s) 1771, 1996
BstV1I GCAGC 7 cut(s) 145, 707, 815, 866, 1184, 2501, 2860
BstV2I GAAGAC 2 cut(s) 1106, 2066
BstX2I RGATCY 4 cut(s) 322, 1219, 2284, 2349
BstYI RGATCY 4 cut(s) 322, 1219, 2284, 2349
BstZ17I GTATAC 1 cut(s) 105
BsuI GTATCC 2 cut(s) 1022, 2426
BsuRI GGCC 8 cut(s) 623, 1272, 1799, 1994, 2307, 2525, 2726, 2940
BtgI CCRYGG 1 cut(s) 2727
BtsCI GGATG 4 cut(s) 182, 403, 1103, 1644
BtsIMutI CAGTG 6 cut(s) 131, 399, 875, 1078, 1868, 2395
BveI ACCTGC 1 cut(s) 2254
Cac8I GCNNGC 5 cut(s) 226, 346, 2305, 2599, 2724
CfoI GCGC 2 cut(s) 143, 2494
Cfr13I GGNCC 8 cut(s) 216, 621, 1257, 1520, 1992, 1993, 2125, 2243
CseI GACGC 2 cut(s) 1829, 1915
CsiI ACCWGGT 1 cut(s) 1785
Csp6I GTAC 4 cut(s) 660, 719, 1805, 2131
CspCI CAANNNNNGTGG 2 cut(s) 1562, 1597
CviQI GTAC 4 cut(s) 660, 719, 1805, 2131
DrdI GACNNNNNNGTC 1 cut(s) 262
DseDI GACNNNNNNGTC 1 cut(s) 262
EaeI YGGCCR 2 cut(s) 1270, 1797
Eam1104I CTCTTC 5 cut(s) 282, 1187, 1377, 1940, 2034
EarI CTCTTC 5 cut(s) 282, 1187, 1377, 1940, 2034
EciI GGCGGA 1 cut(s) 723
Ecl136II GAGCTC 1 cut(s) 1156
Eco130I CCWWGG 4 cut(s) 211, 219, 1107, 2727
Eco147I AGGCCT 1 cut(s) 2307
Eco24I GRGCYC 2 cut(s) 1158, 1996
Eco32I GATATC 2 cut(s) 2050, 2323
Eco47I GGWCC 5 cut(s) 216, 1257, 1520, 2125, 2243
Eco47III AGCGCT 2 cut(s) 142, 2493
Eco53kI GAGCTC 1 cut(s) 1156
Eco57I CTGAAG 3 cut(s) 746, 899, 2015
Eco88I CYCGRG 3 cut(s) 552, 587, 2239
EcoICRI GAGCTC 1 cut(s) 1156
EcoO109I RGGNCCY 4 cut(s) 1257, 1992, 1993, 2243
EcoRI GAATTC 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 1785, 2940
EcoRV GATATC 2 cut(s) 2050, 2323
EcoT14I CCWWGG 4 cut(s) 211, 219, 1107, 2727
EcoT22I ATGCAT 2 cut(s) 1489, 2191
EcoT38I GRGCYC 2 cut(s) 1158, 1996
ErhI CCWWGG 4 cut(s) 211, 219, 1107, 2727
FalI AAGNNNNNCTT 2 cut(s) 1091, 1123
FaqI GGGAC 3 cut(s) 229, 2256, 2853
FauI CCCGC 1 cut(s) 1143
FauNDI CATATG 2 cut(s) 230, 547
FbaI TGATCA 3 cut(s) 435, 2160, 2857
FblI GTMKAC 2 cut(s) 104, 1782
Fnu4HI GCNGC 8 cut(s) 159, 696, 804, 855, 1198, 2393, 2490, 2849
FokI GGATG 4 cut(s) 169, 410, 1090, 1651
FriOI GRGCYC 2 cut(s) 1158, 1996
Fsp4HI GCNGC 8 cut(s) 159, 696, 804, 855, 1198, 2393, 2490, 2849
FspBI CTAG 6 cut(s) 170, 194, 906, 1172, 2598, 2933
GlaI GCGC 2 cut(s) 142, 2493
GluI GCNGC 8 cut(s) 159, 696, 804, 855, 1198, 2393, 2490, 2849
GsaI CCCAGC 1 cut(s) 1588
GsuI CTGGAG 3 cut(s) 1559, 2488, 2552
HaeII RGCGCY 2 cut(s) 144, 2495
HaeIII GGCC 8 cut(s) 623, 1272, 1799, 1994, 2307, 2525, 2726, 2940
HapII CCGG 1 cut(s) 1248
HgaI GACGC 2 cut(s) 1829, 1915
HhaI GCGC 2 cut(s) 143, 2494
Hin6I GCGC 2 cut(s) 141, 2492
HinP1I GCGC 2 cut(s) 141, 2492
HincII GTYRAC 2 cut(s) 267, 1026
HindII GTYRAC 2 cut(s) 267, 1026
HindIII AAGCTT 4 cut(s) 38, 1331, 1349, 2138
HpaII CCGG 1 cut(s) 1248
HphI GGTGA 6 cut(s) 782, 805, 1199, 1559, 1654, 2726
Hpy166II GTNNAC 8 cut(s) 105, 267, 660, 1026, 1661, 1769, 1783, 1792
Hpy8I GTNNAC 8 cut(s) 105, 267, 660, 1026, 1661, 1769, 1783, 1792
Hpy99I CGWCG 3 cut(s) 1325, 1909, 2678
HpyAV CCTTC 8 cut(s) 416, 611, 874, 1184, 1270, 2116, 2563, 2812
HpyCH4III ACNGT 7 cut(s) 191, 394, 1444, 1881, 1931, 2135, 2741
HpyCH4IV ACGT 1 cut(s) 1807
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 860, 911, 1718, 2012, 2309, 2423, 2617
HpySE526I ACGT 1 cut(s) 1807
HspAI GCGC 2 cut(s) 141, 2492
KpnI GGTACC 1 cut(s) 722
Ksp22I TGATCA 3 cut(s) 435, 2160, 2857
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 4 cut(s) 542, 1153, 1534, 2533
Lsp1109I GCAGC 7 cut(s) 145, 707, 815, 866, 1184, 2501, 2860
LweI GCATC 2 cut(s) 1697, 2198
MabI ACCWGGT 1 cut(s) 1785
MaeI CTAG 6 cut(s) 170, 194, 906, 1172, 2598, 2933
MaeII ACGT 1 cut(s) 1807
MaeIII GTNAC 4 cut(s) 502, 649, 751, 2693
MbiI CCGCTC 1 cut(s) 2392
MfeI CAATTG 2 cut(s) 1823, 2264
MflI RGATCY 4 cut(s) 322, 1219, 2284, 2349
MhlI GDGCHC 4 cut(s) 294, 1158, 1771, 1996
MlsI TGGCCA 1 cut(s) 1799
MluNI TGGCCA 1 cut(s) 1799
MlyI GAGTC 4 cut(s) 250, 578, 1550, 1640
MmeI TCCRAC 2 cut(s) 210, 1247
Mox20I TGGCCA 1 cut(s) 1799
Mph1103I ATGCAT 2 cut(s) 1489, 2191
MroXI GAANNNNTTC 2 cut(s) 302, 1701
MscI TGGCCA 1 cut(s) 1799
MslI CAYNNNNRTG 3 cut(s) 893, 2184, 2732
Msp20I TGGCCA 1 cut(s) 1799
MspA1I CMGCKG 2 cut(s) 313, 857
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 1 cut(s) 1248
MspR9I CCNGG 3 cut(s) 1248, 1787, 2942
MunI CAATTG 2 cut(s) 1823, 2264
Mva1269I GAATGC 3 cut(s) 1489, 2279, 2491
MvaI CCWGG 2 cut(s) 1787, 2942
MwoI GCNNNNNNNGC 8 cut(s) 83, 860, 911, 1718, 2012, 2309, 2423, 2617
NciI CCSGG 1 cut(s) 1248
NcoI CCATGG 1 cut(s) 2727
NdeI CATATG 2 cut(s) 230, 547
NheI GCTAGC 1 cut(s) 2597
NlaIV GGNNCC 8 cut(s) 217, 622, 720, 1819, 1993, 1994, 1995, 2244
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 751, 2693
NsiI ATGCAT 2 cut(s) 1489, 2191
NspI RCATGY 5 cut(s) 133, 854, 1233, 1504, 2380
PaeR7I CTCGAG 1 cut(s) 587
PceI AGGCCT 1 cut(s) 2307
PciI ACATGT 2 cut(s) 1229, 2376
PciSI GCTCTTC 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 1317
PctI GAATGC 3 cut(s) 1489, 2279, 2491
PdmI GAANNNNTTC 2 cut(s) 302, 1701
PkrI GCNGC 8 cut(s) 160, 697, 805, 856, 1199, 2394, 2491, 2850
PleI GAGTC 4 cut(s) 250, 577, 1549, 1639
PpsI GAGTC 4 cut(s) 250, 577, 1549, 1639
Ppu21I YACGTR 1 cut(s) 1808
PpuMI RGGWCCY 2 cut(s) 1257, 2243
PscI ACATGT 2 cut(s) 1229, 2376
PshBI ATTAAT 3 cut(s) 645, 729, 1673
Psp124BI GAGCTC 1 cut(s) 1158
Psp5II RGGWCCY 2 cut(s) 1257, 2243
Psp6I CCWGG 2 cut(s) 1785, 2940
PspFI CCCAGC 1 cut(s) 1584
PspGI CCWGG 2 cut(s) 1785, 2940
PspN4I GGNNCC 8 cut(s) 217, 622, 720, 1819, 1993, 1994, 1995, 2244
PspOMI GGGCCC 1 cut(s) 1992
PspPI GGNCC 8 cut(s) 216, 621, 1257, 1520, 1992, 1993, 2125, 2243
PspPPI RGGWCCY 2 cut(s) 1257, 2243
PsuI RGATCY 4 cut(s) 322, 1219, 2284, 2349
PvuII CAGCTG 2 cut(s) 313, 857
RsaI GTAC 4 cut(s) 661, 720, 1806, 2132
RsaNI GTAC 4 cut(s) 660, 719, 1805, 2131
RseI CAYNNNNRTG 3 cut(s) 893, 2184, 2732
SacI GAGCTC 1 cut(s) 1158
SapI GCTCTTC 1 cut(s) 282
SatI GCNGC 8 cut(s) 159, 696, 804, 855, 1198, 2393, 2490, 2849
Sau96I GGNCC 8 cut(s) 216, 621, 1257, 1520, 1992, 1993, 2125, 2243
ScaI AGTACT 1 cut(s) 2132
SchI GAGTC 4 cut(s) 250, 578, 1550, 1640
ScrFI CCNGG 3 cut(s) 1248, 1787, 2942
SduI GDGCHC 4 cut(s) 294, 1158, 1771, 1996
SexAI ACCWGGT 1 cut(s) 1785
SfaNI GCATC 2 cut(s) 1697, 2198
SfcI CTRYAG 1 cut(s) 2095
Sfr274I CTCGAG 1 cut(s) 587
SinI GGWCC 5 cut(s) 216, 1257, 1520, 2125, 2243
SlaI CTCGAG 1 cut(s) 587
SmiMI CAYNNNNRTG 3 cut(s) 893, 2184, 2732
SmlI CTYRAG 6 cut(s) 32, 279, 587, 1157, 1553, 2564
SmoI CTYRAG 6 cut(s) 32, 279, 587, 1157, 1553, 2564
SpeI ACTAGT 1 cut(s) 2932
SseBI AGGCCT 1 cut(s) 2307
SsiI CCGC 4 cut(s) 708, 1150, 2392, 2687
SspMI CTAG 6 cut(s) 170, 194, 906, 1172, 2598, 2933
SstI GAGCTC 1 cut(s) 1158
StuI AGGCCT 1 cut(s) 2307
StyD4I CCNGG 3 cut(s) 1246, 1785, 2940
StyI CCWWGG 4 cut(s) 211, 219, 1107, 2727
TaaI ACNGT 7 cut(s) 191, 394, 1444, 1881, 1931, 2135, 2741
TaiI ACGT 1 cut(s) 1810
TaqI TCGA 2 cut(s) 588, 1311
TatI WGTACW 2 cut(s) 659, 2130
TauI GCSGC 1 cut(s) 2395
TscAI CASTG 6 cut(s) 131, 399, 882, 1078, 1875, 2402
TseFI GTSAC 2 cut(s) 751, 2693
TseI GCWGC 7 cut(s) 158, 695, 803, 854, 1197, 2489, 2848
Tsp45I GTSAC 2 cut(s) 751, 2693
TspGWI ACGGA 1 cut(s) 1690
TspRI CASTG 6 cut(s) 131, 399, 882, 1078, 1875, 2402
Vha464I CTTAAG 1 cut(s) 32
VneI GTGCAC 1 cut(s) 1767
VpaK11BI GGWCC 5 cut(s) 216, 1257, 1520, 2125, 2243
VspI ATTAAT 3 cut(s) 645, 729, 1673
XapI RAATTY 7 cut(s) 465, 1204, 1558, 1873, 1945, 2768, 2922
XbaI TCTAGA 1 cut(s) 1171
XceI RCATGY 5 cut(s) 133, 854, 1233, 1504, 2380
XcmI CCANNNNNNNNNTGG 1 cut(s) 1631
XhoI CTCGAG 1 cut(s) 587
XmiI GTMKAC 2 cut(s) 104, 1782
XmnI GAANNNNTTC 2 cut(s) 302, 1701
XspI CTAG 6 cut(s) 170, 194, 906, 1172, 2598, 2933
ZrmI AGTACT 1 cut(s) 2132
Zsp2I ATGCAT 2 cut(s) 1489, 2191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.