FvH4_4g05920

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004710
Physical Location & Seq
Reverse (-)
5344532 .. 5348817
4286 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05920.t1

Sequence Viewer

Length: 1026 bp
ATGAACCGGATCGCGGCGAGGTCGGTGGCCGGAGCTCTGCGCCATGGCCGCAACTTCAACCGCCACATTTCCACTGCTACCGTCGTTAGTACTACGCCGAAGTTCGACGCAGCCGACTATCTCCACCGCCAGAGCGCCACCGCTTTCGGCTCCTGGAGGATGATGAGCACGGTGACGCCGTCGATTCAAACGCCGCCTCCTTCGGCTGAGAAGGAGAAGGACAAGAAGGAGAGCTCCGGCTCCGGCTCCGACGCGAAGAAAGACGTCGTGATTTCGAATTATTGGGGAATTGCGAGGCCGAAGCTCACCAGGGAGGACGGCACTGAGTGGCCATGGAATTGCTTCATGCCATGGGAGACGTATGAAGCAGATTTGTCGATCGATCTGAGCAAGCACCATGTGCCGAAGACGTTTCTGGACAAAGTGGCGTACAGAACGGTCAAGCTTCTTCGAGTTCCGACTGATATCTTCTTCCAGAGACGGTATGGGTGCCGTGCAATGATGCTGGAAACTGTGGCAGCTGTCCCTGGTATGGTTGGAGGAATGCTGCTGCACTTAAGGTCTCTCCGAAAGTTTCAGCAGAGTGGTGGTTGGATCAAAGCCTTACTTGAAGAAGCAGAGAATGAGAGGATGCACTTGATGACAATGGTGGAACTTGTGAAGCCTGCATGGTATGAGAGGCTACTTGTTCTCACTGTGCAGGGAGTCTTCTTCAATGCCTTCTTCGGGCTCTATATGATTTCCCCCAAGGTTGCACACAGGGTTGTTGGATATCTGGAGGAGGAGGCTATTCACTCTTATACAGAGTACTTGAAGGATATTGATAGTGGCAAGATTGAAAATGTACCGGCCCCTGCTATAGCAATTGACTACTGGAGGCTACCAAAAGATGCTAAACTGAAGGATGTTATCACTGTGATTCGTGCTGATGAAGCTCACCATAGGGATGTCAACCATTTTGCATCTGATATTCATCATCAGGGAAAGGAACTGAGAGATGCCCCAGCTCCCATTGGATATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AOX

Protein Analysis

342

Amino Acids

38.76

Weight (kDa)

8.99

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 130 - 322 7.1e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 489
AccII CGCG 2 cut(s) 14, 254
AciI CCGC 6 cut(s) 14, 49, 61, 127, 141, 194
AclWI GGATC 2 cut(s) 17, 602
AcoI YGGCCR 3 cut(s) 27, 46, 329
AcuI CTGAAG 1 cut(s) 920
AcyI GRCGYC 2 cut(s) 176, 264
AdeI CACNNNGTG 2 cut(s) 327, 400
AfaI GTAC 4 cut(s) 91, 431, 809, 846
AfiI CCNNNNNNNGG 3 cut(s) 13, 532, 726
AflII CTTAAG 1 cut(s) 556
AgsI TTSAA 6 cut(s) 58, 188, 611, 715, 814, 839
AjnI CCWGG 3 cut(s) 152, 308, 526
AluBI AGCT 7 cut(s) 35, 234, 304, 445, 521, 935, 1007
AluI AGCT 7 cut(s) 35, 234, 304, 445, 521, 935, 1007
Alw21I GWGCWC 3 cut(s) 37, 170, 236
Alw26I GTCTC 3 cut(s) 350, 472, 567
AlwI GGATC 2 cut(s) 17, 602
AoxI GGCC 5 cut(s) 27, 46, 296, 329, 849
ApeKI GCWGC 4 cut(s) 110, 518, 547, 550
Asp700I GAANNNNTTC 1 cut(s) 341
AspLEI GCGC 2 cut(s) 42, 137
AspS9I GGNCC 1 cut(s) 850
AsuHPI GGTGA 3 cut(s) 184, 298, 929
AsuII TTCGAA 1 cut(s) 275
BalI TGGCCA 1 cut(s) 331
BanI GGYRCC 1 cut(s) 489
BanII GRGCYC 3 cut(s) 37, 236, 732
BbsI GAAGAC 2 cut(s) 413, 700
Bbv12I GWGCWC 3 cut(s) 37, 170, 236
BbvI GCAGC 4 cut(s) 122, 530, 534, 537
BceAI ACGGC 3 cut(s) 163, 334, 477
BcgI CGANNNNNNTGC 2 cut(s) 357, 391
BciT130I CCWGG 3 cut(s) 154, 310, 528
BcoDI GTCTC 3 cut(s) 350, 472, 567
BfmI CTRYAG 1 cut(s) 858
BfoI RGCGCY 1 cut(s) 138
BfrI CTTAAG 1 cut(s) 556
BisI GCNGC 7 cut(s) 15, 49, 111, 194, 519, 548, 551
BlsI GCNGC 7 cut(s) 16, 50, 112, 195, 520, 549, 552
BmcAI AGTACT 2 cut(s) 91, 809
Bme1390I CCNGG 3 cut(s) 154, 310, 528
BmgT120I GGNCC 1 cut(s) 850
BmiI GGNNCC 5 cut(s) 151, 241, 247, 491, 852
BmrFI CCNGG 3 cut(s) 154, 310, 528
BmsI GCATC 5 cut(s) 492, 621, 880, 971, 988
BpiI GAAGAC 2 cut(s) 413, 700
BpmI CTGGAG 3 cut(s) 175, 797, 895
Bpu14I TTCGAA 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 381
BsaBI GATNNNNATC 1 cut(s) 972
BsaHI GRCGYC 2 cut(s) 176, 264
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 6 cut(s) 43, 309, 332, 350, 526, 747
BsaWI WCCGGW 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 532, 726
Bse118I RCCGGY 1 cut(s) 847
Bse1I ACTGG 1 cut(s) 878
Bse3DI GCAATG 1 cut(s) 504
Bse8I GATNNNNATC 1 cut(s) 972
BseBI CCWGG 3 cut(s) 154, 310, 528
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 6 cut(s) 43, 309, 332, 350, 526, 747
BseGI GGATG 4 cut(s) 165, 636, 910, 952
BseJI GATNNNNATC 1 cut(s) 972
BseLI CCNNNNNNNGG 3 cut(s) 13, 532, 726
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 4 cut(s) 198, 315, 377, 983
BseNI ACTGG 1 cut(s) 878
BseRI GAGGAG 2 cut(s) 794, 797
BseXI GCAGC 4 cut(s) 122, 530, 534, 537
BseYI CCCAGC 1 cut(s) 1003
BsgI GTGCAG 2 cut(s) 536, 719
Bsh1236I CGCG 2 cut(s) 14, 254
Bsh1285I CGRYCG 1 cut(s) 381
BshFI GGCC 5 cut(s) 29, 48, 298, 331, 851
BshNI GGYRCC 1 cut(s) 489
BshVI ATCGAT 1 cut(s) 381
BsiEI CGRYCG 1 cut(s) 381
BsiHKAI GWGCWC 3 cut(s) 37, 170, 236
BsiSI CCGG 5 cut(s) 7, 30, 237, 243, 848
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 3 cut(s) 13, 532, 726
BsmAI GTCTC 3 cut(s) 350, 472, 567
BsmBI CGTCTC 2 cut(s) 350, 472
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 5 cut(s) 29, 48, 298, 331, 851
Bso31I GGTCTC 1 cut(s) 567
Bsp119I TTCGAA 1 cut(s) 275
Bsp1286I GDGCHC 4 cut(s) 37, 170, 236, 732
Bsp143I GATC 4 cut(s) 9, 378, 382, 594
Bsp19I CCATGG 3 cut(s) 43, 332, 350
BspACI CCGC 6 cut(s) 14, 49, 61, 127, 141, 194
BspANI GGCC 5 cut(s) 29, 48, 298, 331, 851
BspCNI CTCAG 4 cut(s) 199, 316, 378, 984
BspDI ATCGAT 1 cut(s) 381
BspFNI CGCG 2 cut(s) 14, 254
BspLI GGNNCC 5 cut(s) 151, 241, 247, 491, 852
BspPI GGATC 2 cut(s) 17, 602
BspT104I TTCGAA 1 cut(s) 275
BspT107I GGYRCC 1 cut(s) 489
BspTI CTTAAG 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 504
BsrFI RCCGGY 1 cut(s) 847
BsrI ACTGG 1 cut(s) 878
BssAI RCCGGY 1 cut(s) 847
BssECI CCNNGG 6 cut(s) 43, 309, 332, 350, 526, 747
BssMI GATC 4 cut(s) 9, 378, 382, 594
BssNI GRCGYC 2 cut(s) 176, 264
BssT1I CCWWGG 4 cut(s) 43, 332, 350, 747
Bst2UI CCWGG 3 cut(s) 154, 310, 528
Bst4CI ACNGT 7 cut(s) 82, 172, 439, 483, 514, 697, 916
BstACI GRCGYC 2 cut(s) 176, 264
BstAFI CTTAAG 1 cut(s) 556
BstAPI GCANNNNNTGC 1 cut(s) 400
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 392, 666
BstDEI CTNAG 4 cut(s) 207, 324, 386, 992
BstDSI CCRYGG 3 cut(s) 43, 332, 350
BstF5I GGATG 4 cut(s) 165, 636, 910, 952
BstFNI CGCG 2 cut(s) 14, 254
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 2 cut(s) 42, 137
BstKTI GATC 4 cut(s) 12, 381, 385, 597
BstMAI GTCTC 3 cut(s) 350, 472, 567
BstMBI GATC 4 cut(s) 9, 378, 382, 594
BstMCI CGRYCG 1 cut(s) 381
BstMWI GCNNNNNNNGC 3 cut(s) 48, 400, 932
BstNI CCWGG 3 cut(s) 154, 310, 528
BstSCI CCNGG 3 cut(s) 152, 308, 526
BstSFI CTRYAG 1 cut(s) 858
BstUI CGCG 2 cut(s) 14, 254
BstV1I GCAGC 4 cut(s) 122, 530, 534, 537
BstV2I GAAGAC 2 cut(s) 413, 700
Bsu15I ATCGAT 1 cut(s) 381
BsuRI GGCC 5 cut(s) 29, 48, 298, 331, 851
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 3 cut(s) 43, 332, 350
BtsCI GGATG 4 cut(s) 165, 636, 910, 952
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 4 cut(s) 72, 321, 693, 912
Cac8I GCNNGC 2 cut(s) 392, 666
CfoI GCGC 2 cut(s) 42, 137
Cfr10I RCCGGY 1 cut(s) 847
Cfr13I GGNCC 1 cut(s) 850
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 3 cut(s) 116, 184, 260
Csp6I GTAC 4 cut(s) 90, 430, 808, 845
CviAII CATG 6 cut(s) 44, 333, 346, 351, 398, 669
CviQI GTAC 4 cut(s) 90, 430, 808, 845
DdeI CTNAG 4 cut(s) 207, 324, 386, 992
DpnI GATC 4 cut(s) 11, 380, 384, 596
DpnII GATC 4 cut(s) 9, 378, 382, 594
DraIII CACNNNGTG 2 cut(s) 327, 400
EaeI YGGCCR 3 cut(s) 27, 46, 329
Ecl136II GAGCTC 2 cut(s) 35, 234
Eco130I CCWWGG 4 cut(s) 43, 332, 350, 747
Eco24I GRGCYC 3 cut(s) 37, 236, 732
Eco31I GGTCTC 1 cut(s) 567
Eco32I GATATC 3 cut(s) 466, 773, 1019
Eco53kI GAGCTC 2 cut(s) 35, 234
Eco57I CTGAAG 1 cut(s) 920
EcoICRI GAGCTC 2 cut(s) 35, 234
EcoRII CCWGG 3 cut(s) 152, 308, 526
EcoRV GATATC 3 cut(s) 466, 773, 1019
EcoT14I CCWWGG 4 cut(s) 43, 332, 350, 747
EcoT38I GRGCYC 3 cut(s) 37, 236, 732
ErhI CCWWGG 4 cut(s) 43, 332, 350, 747
Esp3I CGTCTC 2 cut(s) 350, 472
FaeI CATG 6 cut(s) 47, 336, 349, 354, 401, 672
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 1 cut(s) 509
FatI CATG 6 cut(s) 43, 332, 345, 350, 397, 668
Fnu4HI GCNGC 7 cut(s) 15, 49, 111, 194, 519, 548, 551
FokI GGATG 4 cut(s) 172, 643, 917, 959
FriOI GRGCYC 3 cut(s) 37, 236, 732
Fsp4HI GCNGC 7 cut(s) 15, 49, 111, 194, 519, 548, 551
GlaI GCGC 2 cut(s) 41, 136
GluI GCNGC 7 cut(s) 15, 49, 111, 194, 519, 548, 551
GsaI CCCAGC 1 cut(s) 1007
GsuI CTGGAG 3 cut(s) 175, 797, 895
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 5 cut(s) 29, 48, 298, 331, 851
HapII CCGG 5 cut(s) 7, 30, 237, 243, 848
HgaI GACGC 3 cut(s) 116, 184, 260
HhaI GCGC 2 cut(s) 42, 137
Hin1I GRCGYC 2 cut(s) 176, 264
Hin1II CATG 6 cut(s) 47, 336, 349, 354, 401, 672
Hin6I GCGC 2 cut(s) 40, 135
HinP1I GCGC 2 cut(s) 40, 135
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 3 cut(s) 184, 705, 919
HpaII CCGG 5 cut(s) 7, 30, 237, 243, 848
HphI GGTGA 3 cut(s) 184, 298, 929
Hpy166II GTNNAC 1 cut(s) 952
Hpy188I TCNGA 5 cut(s) 250, 387, 459, 569, 967
Hpy188III TCNNGA 4 cut(s) 268, 416, 475, 776
Hpy8I GTNNAC 1 cut(s) 952
Hpy99I CGWCG 5 cut(s) 86, 110, 184, 254, 269
HpyAV CCTTC 7 cut(s) 205, 210, 211, 220, 730, 808, 895
HpyCH4III ACNGT 7 cut(s) 82, 172, 439, 483, 514, 697, 916
HpyCH4IV ACGT 3 cut(s) 264, 359, 410
HpyCH4V TGCA 7 cut(s) 497, 553, 634, 668, 700, 755, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 400, 932
HpyF3I CTNAG 4 cut(s) 207, 324, 386, 992
HpySE526I ACGT 3 cut(s) 264, 359, 410
Hsp92I GRCGYC 2 cut(s) 176, 264
Hsp92II CATG 6 cut(s) 47, 336, 349, 354, 401, 672
HspAI GCGC 2 cut(s) 40, 135
Kzo9I GATC 4 cut(s) 9, 378, 382, 594
LmnI GCTCC 6 cut(s) 32, 155, 239, 245, 251, 1012
Lsp1109I GCAGC 4 cut(s) 122, 530, 534, 537
LweI GCATC 5 cut(s) 492, 621, 880, 971, 988
MaeII ACGT 3 cut(s) 264, 359, 410
MaeIII GTNAC 1 cut(s) 172
MalI GATC 4 cut(s) 11, 380, 384, 596
MboI GATC 4 cut(s) 9, 378, 382, 594
MboII GAAGA 9 cut(s) 268, 418, 440, 460, 463, 623, 700, 703, 715
MfeI CAATTG 1 cut(s) 864
MhlI GDGCHC 4 cut(s) 37, 170, 236, 732
MlsI TGGCCA 1 cut(s) 331
MluCI AATT 4 cut(s) 277, 288, 337, 864
MluNI TGGCCA 1 cut(s) 331
MlyI GAGTC 1 cut(s) 714
MmeI TCCRAC 5 cut(s) 273, 482, 517, 572, 748
Mox20I TGGCCA 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 341
MscI TGGCCA 1 cut(s) 331
MseI TTAA 1 cut(s) 557
MslI CAYNNNNRTG 1 cut(s) 945
Msp20I TGGCCA 1 cut(s) 331
MspA1I CMGCKG 1 cut(s) 521
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 5 cut(s) 7, 30, 237, 243, 848
MspR9I CCNGG 3 cut(s) 154, 310, 528
MunI CAATTG 1 cut(s) 864
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 3 cut(s) 154, 310, 528
MvnI CGCG 2 cut(s) 14, 254
MwoI GCNNNNNNNGC 3 cut(s) 48, 400, 932
NcoI CCATGG 3 cut(s) 43, 332, 350
NdeII GATC 4 cut(s) 9, 378, 382, 594
NlaIII CATG 6 cut(s) 47, 336, 349, 354, 401, 672
NlaIV GGNNCC 5 cut(s) 151, 241, 247, 491, 852
NmuCI GTSAC 1 cut(s) 172
NspV TTCGAA 1 cut(s) 275
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 176
PctI GAATGC 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 341
PfeI GAWTC 2 cut(s) 184, 919
PflFI GACNNNGTC 1 cut(s) 178
PfoI TCCNGGA 1 cut(s) 152
PkrI GCNGC 7 cut(s) 16, 50, 112, 195, 520, 549, 552
Ple19I CGATCG 1 cut(s) 381
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
Psp124BI GAGCTC 2 cut(s) 37, 236
Psp6I CCWGG 3 cut(s) 152, 308, 526
PspFI CCCAGC 1 cut(s) 1003
PspGI CCWGG 3 cut(s) 152, 308, 526
PspN4I GGNNCC 5 cut(s) 151, 241, 247, 491, 852
PspPI GGNCC 1 cut(s) 850
PsyI GACNNNGTC 1 cut(s) 178
PvuI CGATCG 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 4 cut(s) 91, 431, 809, 846
RsaNI GTAC 4 cut(s) 90, 430, 808, 845
RseI CAYNNNNRTG 1 cut(s) 945
SacI GAGCTC 2 cut(s) 37, 236
SaqAI TTAA 1 cut(s) 557
SatI GCNGC 7 cut(s) 15, 49, 111, 194, 519, 548, 551
Sau3AI GATC 4 cut(s) 9, 378, 382, 594
Sau96I GGNCC 1 cut(s) 850
ScaI AGTACT 2 cut(s) 91, 809
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 3 cut(s) 154, 310, 528
SduI GDGCHC 4 cut(s) 37, 170, 236, 732
SfaNI GCATC 5 cut(s) 492, 621, 880, 971, 988
SfcI CTRYAG 1 cut(s) 858
SfuI TTCGAA 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 1 cut(s) 556
SmoI CTYRAG 1 cut(s) 556
Sse9I AATT 4 cut(s) 277, 288, 337, 864
SsiI CCGC 6 cut(s) 14, 49, 61, 127, 141, 194
SstI GAGCTC 2 cut(s) 37, 236
StyD4I CCNGG 3 cut(s) 152, 308, 526
StyI CCWWGG 4 cut(s) 43, 332, 350, 747
TaaI ACNGT 7 cut(s) 82, 172, 439, 483, 514, 697, 916
TaiI ACGT 3 cut(s) 267, 362, 413
TaqI TCGA 6 cut(s) 105, 182, 275, 377, 381, 451
TasI AATT 4 cut(s) 277, 288, 337, 864
TatI WGTACW 2 cut(s) 89, 807
TauI GCSGC 3 cut(s) 17, 51, 196
TfiI GAWTC 2 cut(s) 184, 919
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 4 cut(s) 79, 328, 700, 919
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 4 cut(s) 110, 518, 547, 550
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 5 cut(s) 17, 334, 378, 945, 962
TspRI CASTG 4 cut(s) 79, 328, 700, 919
Tth111I GACNNNGTC 1 cut(s) 178
Vha464I CTTAAG 1 cut(s) 556
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 341
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 2 cut(s) 91, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.