Rroxscaffold_5G00342060

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004710
Physical Location & Seq
Reverse (-)
10805471 .. 10807900
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342060.1

Sequence Viewer

Length: 1041 bp
ATGAACCGGATCGCTGCGAGGTCGGTGGCCGGAGCTCTGCGCCATGGCCGGAACTTCAACCGCCACATTTCGACGGTGCCGGTCGCTACAAGGACTTTGGACACCGCCGATTATCTTAACCGCCAGAACGCTACCGCTTTCGGCTCATTTTACTCCACCAGGATGATGAGCTCGGTGACGCCATCCGTCCAAACGCCGCCGCCGCCTCCGGCGGAGAAGGACCAGAAGGAGAGCTCCGCCTCCGACGCCAAGAAAGACGCCGTCGTTTCACATTATTGGGGGATTAAGAGGCCAGAGATCAGAAGGGAGGACGGCTCTGAGTGGCCATGGAATTGCTTCATGCCATGGGAGACTTATCAAGCAGATTTATCGATCGATCTGAGCAAGCACCATGTGCCGAAGACGTTTCTGGACAAAGTGGCTTACAGAACAGTCAAGCTTCTTCGAGTTCCAACTGATATCTTCTTTCAGAGACGGTATGGGTGCCGTGCAATGATGCTGGAAACTGTGGCAGCTGTCCCTGGTATGGTTGGAGGAATGCTGCTGCACTTAAGGTCTCTCCGAAAGTTCCAGCAGAGTGGTGGTTGGATCAAAGCCTTACTTGAAGAAGCAGAGAATGAGAGGATGCACTTGATGACAATGGTGGAACTCGTGAAGCCTGCATGGTACGAGAGGCTACTGGTTCTCACTGTGCAGGGAGTCTTCTTCAATGCCTTCTTTGTGCTCTATATGATTTCCCCCAAGCTAGCACACCGGGTTGTTGGATATCTAGAAGAGGAGGCTATTCACTCTTATACAGAGTACTTGAAGGATATTGATGGTGGCAAAATTGAAAATGTACCAGCTCCTGCTATAGCAATTGACTACTGGAGGCTGCCAAAAGATGCTACACTTAAGGATGTTATCACTGTGATTCGTGCAGATGAAGCTCACCATAGGGATGTCAACCATTTTGCATCTGTGAGTATCAAAGCTTTTCATAATTCCATGTTCTTTATTGTTCTACAAATTTATAACTGTCTCAGCGGTTCAAGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AOX

Protein Analysis

346

Amino Acids

39.54

Weight (kDa)

9.19

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 128 - 318 5.5e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1014
AccB1I GGYRCC 2 cut(s) 76, 483
AclWI GGATC 2 cut(s) 17, 596
AcoI YGGCCR 3 cut(s) 27, 46, 323
AcsI RAATTY 1 cut(s) 1008
AcyI GRCGYC 3 cut(s) 179, 246, 258
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 3 cut(s) 668, 803, 840
AfiI CCNNNNNNNGG 1 cut(s) 526
AflII CTTAAG 2 cut(s) 550, 893
AgsI TTSAA 6 cut(s) 58, 605, 709, 808, 833, 1032
AjnI CCWGG 2 cut(s) 158, 520
AluBI AGCT 9 cut(s) 35, 171, 234, 439, 515, 745, 845, 929, 974
AluI AGCT 9 cut(s) 35, 171, 234, 439, 515, 745, 845, 929, 974
Alw21I GWGCWC 4 cut(s) 37, 173, 236, 726
Alw26I GTCTC 4 cut(s) 344, 466, 561, 1025
AlwI GGATC 2 cut(s) 17, 596
AlwNI CAGNNNCTG 1 cut(s) 848
AoxI GGCC 4 cut(s) 27, 46, 290, 323
ApeKI GCWGC 5 cut(s) 14, 512, 541, 544, 874
ApoI RAATTY 1 cut(s) 1008
Asp700I GAANNNNTTC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 220
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 2 cut(s) 187, 923
AsuNHI GCTAGC 1 cut(s) 745
AvaII GGWCC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 325
BanI GGYRCC 2 cut(s) 76, 483
BanII GRGCYC 3 cut(s) 37, 173, 236
BauI CACGAG 1 cut(s) 650
BbsI GAAGAC 2 cut(s) 407, 694
Bbv12I GWGCWC 4 cut(s) 37, 173, 236, 726
BbvI GCAGC 4 cut(s) 524, 528, 531, 861
BccI CCATC 2 cut(s) 190, 812
BceAI ACGGC 3 cut(s) 245, 328, 471
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 2 cut(s) 160, 522
BcnI CCSGG 1 cut(s) 755
BcoDI GTCTC 4 cut(s) 344, 466, 561, 1025
BfaI CTAG 2 cut(s) 746, 770
BfmI CTRYAG 1 cut(s) 852
BfrI CTTAAG 2 cut(s) 550, 893
BisI GCNGC 8 cut(s) 15, 197, 200, 203, 513, 542, 545, 875
BlsI GCNGC 8 cut(s) 16, 198, 201, 204, 514, 543, 546, 876
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 3 cut(s) 160, 522, 755
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 2 cut(s) 78, 485
BmrFI CCNGG 3 cut(s) 160, 522, 755
BmsI GCATC 4 cut(s) 486, 615, 874, 965
BmtI GCTAGC 1 cut(s) 749
BpiI GAAGAC 2 cut(s) 407, 694
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 1 cut(s) 889
BpuMI CCSGG 1 cut(s) 755
Bsa29I ATCGAT 2 cut(s) 371, 375
BsaHI GRCGYC 3 cut(s) 179, 246, 258
BsaI GGTCTC 1 cut(s) 561
BsaJI CCNNGG 4 cut(s) 43, 326, 344, 520
BsaWI WCCGGW 1 cut(s) 6
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 684, 872
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 2 cut(s) 160, 522
BseCI ATCGAT 2 cut(s) 371, 375
BseDI CCNNGG 4 cut(s) 43, 326, 344, 520
BseGI GGATG 5 cut(s) 168, 182, 630, 904, 946
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 3 cut(s) 309, 371, 1036
BseNI ACTGG 2 cut(s) 684, 872
BseRI GAGGAG 1 cut(s) 791
BseXI GCAGC 4 cut(s) 524, 528, 531, 861
BsgI GTGCAG 3 cut(s) 530, 713, 939
Bsh1285I CGRYCG 2 cut(s) 84, 375
BshFI GGCC 4 cut(s) 29, 48, 292, 325
BshNI GGYRCC 2 cut(s) 76, 483
BshVI ATCGAT 2 cut(s) 371, 375
BsiEI CGRYCG 2 cut(s) 84, 375
BsiHKAI GWGCWC 4 cut(s) 37, 173, 236, 726
BsiSI CCGG 6 cut(s) 7, 30, 49, 80, 209, 754
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 4 cut(s) 344, 466, 561, 1025
BsmBI CGTCTC 1 cut(s) 466
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 4 cut(s) 29, 48, 292, 325
Bso31I GGTCTC 1 cut(s) 561
Bsp1286I GDGCHC 4 cut(s) 37, 173, 236, 726
Bsp143I GATC 5 cut(s) 9, 297, 372, 376, 588
Bsp19I CCATGG 3 cut(s) 43, 326, 344
BspANI GGCC 4 cut(s) 29, 48, 292, 325
BspCNI CTCAG 3 cut(s) 310, 372, 1035
BspDI ATCGAT 2 cut(s) 371, 375
BspLI GGNNCC 2 cut(s) 78, 485
BspOI GCTAGC 1 cut(s) 749
BspPI GGATC 2 cut(s) 17, 596
BspT107I GGYRCC 2 cut(s) 76, 483
BspTI CTTAAG 2 cut(s) 550, 893
BspTNI GGTCTC 1 cut(s) 561
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 2 cut(s) 684, 872
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 4 cut(s) 43, 326, 344, 520
BssMI GATC 5 cut(s) 9, 297, 372, 376, 588
BssNI GRCGYC 3 cut(s) 179, 246, 258
BssSI CACGAG 1 cut(s) 650
BssT1I CCWWGG 3 cut(s) 43, 326, 344
Bst2BI CACGAG 1 cut(s) 650
Bst2UI CCWGG 2 cut(s) 160, 522
Bst4CI ACNGT 7 cut(s) 76, 433, 477, 508, 691, 910, 1019
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 3 cut(s) 179, 246, 258
BstAFI CTTAAG 2 cut(s) 550, 893
BstAPI GCANNNNNTGC 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 386, 660, 747
BstDEI CTNAG 3 cut(s) 318, 380, 1022
BstDSI CCRYGG 3 cut(s) 43, 326, 344
BstF5I GGATG 5 cut(s) 168, 182, 630, 904, 946
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 5 cut(s) 12, 300, 375, 379, 591
BstMAI GTCTC 4 cut(s) 344, 466, 561, 1025
BstMBI GATC 5 cut(s) 9, 297, 372, 376, 588
BstMCI CGRYCG 2 cut(s) 84, 375
BstMWI GCNNNNNNNGC 4 cut(s) 202, 245, 394, 926
BstNI CCWGG 2 cut(s) 160, 522
BstSCI CCNGG 3 cut(s) 158, 520, 753
BstSFI CTRYAG 1 cut(s) 852
BstV1I GCAGC 4 cut(s) 524, 528, 531, 861
BstV2I GAAGAC 2 cut(s) 407, 694
BstXI CCANNNNNNTGG 1 cut(s) 578
Bsu15I ATCGAT 2 cut(s) 371, 375
BsuRI GGCC 4 cut(s) 29, 48, 292, 325
BsuTUI ATCGAT 2 cut(s) 371, 375
BtgI CCRYGG 3 cut(s) 43, 326, 344
BtsCI GGATG 5 cut(s) 168, 182, 630, 904, 946
BtsIMutI CAGTG 2 cut(s) 687, 906
Cac8I GCNNGC 3 cut(s) 386, 660, 747
CaiI CAGNNNCTG 1 cut(s) 848
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 220
ClaI ATCGAT 2 cut(s) 371, 375
CseI GACGC 3 cut(s) 187, 254, 266
Csp6I GTAC 3 cut(s) 667, 802, 839
CviAII CATG 7 cut(s) 44, 327, 340, 345, 392, 663, 988
CviQI GTAC 3 cut(s) 667, 802, 839
DdeI CTNAG 3 cut(s) 318, 380, 1022
DpnI GATC 5 cut(s) 11, 299, 374, 378, 590
DpnII GATC 5 cut(s) 9, 297, 372, 376, 588
DraIII CACNNNGTG 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 27, 46, 323
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
EciI GGCGGA 2 cut(s) 226, 227
Ecl136II GAGCTC 3 cut(s) 35, 171, 234
Eco130I CCWWGG 3 cut(s) 43, 326, 344
Eco24I GRGCYC 3 cut(s) 37, 173, 236
Eco31I GGTCTC 1 cut(s) 561
Eco32I GATATC 2 cut(s) 460, 767
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 3 cut(s) 35, 171, 234
EcoICRI GAGCTC 3 cut(s) 35, 171, 234
EcoRII CCWGG 2 cut(s) 158, 520
EcoRV GATATC 2 cut(s) 460, 767
EcoT14I CCWWGG 3 cut(s) 43, 326, 344
EcoT38I GRGCYC 3 cut(s) 37, 173, 236
ErhI CCWWGG 3 cut(s) 43, 326, 344
Esp3I CGTCTC 1 cut(s) 466
FaeI CATG 7 cut(s) 47, 330, 343, 348, 395, 666, 991
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 1 cut(s) 503
FatI CATG 7 cut(s) 43, 326, 339, 344, 391, 662, 987
Fnu4HI GCNGC 8 cut(s) 15, 197, 200, 203, 513, 542, 545, 875
FokI GGATG 5 cut(s) 169, 175, 637, 911, 953
FriOI GRGCYC 3 cut(s) 37, 173, 236
Fsp4HI GCNGC 8 cut(s) 15, 197, 200, 203, 513, 542, 545, 875
FspBI CTAG 2 cut(s) 746, 770
GlaI GCGC 1 cut(s) 41
GluI GCNGC 8 cut(s) 15, 197, 200, 203, 513, 542, 545, 875
GsuI CTGGAG 1 cut(s) 889
HaeIII GGCC 4 cut(s) 29, 48, 292, 325
HapII CCGG 6 cut(s) 7, 30, 49, 80, 209, 754
HgaI GACGC 3 cut(s) 187, 254, 266
HhaI GCGC 1 cut(s) 42
Hin1I GRCGYC 3 cut(s) 179, 246, 258
Hin1II CATG 7 cut(s) 47, 330, 343, 348, 395, 666, 991
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HindIII AAGCTT 2 cut(s) 437, 972
HinfI GANTC 2 cut(s) 699, 913
HpaII CCGG 6 cut(s) 7, 30, 49, 80, 209, 754
HphI GGTGA 2 cut(s) 187, 923
Hpy166II GTNNAC 1 cut(s) 946
Hpy188I TCNGA 6 cut(s) 244, 302, 319, 381, 471, 563
Hpy188III TCNNGA 4 cut(s) 410, 652, 770, 1032
Hpy8I GTNNAC 1 cut(s) 946
Hpy99I CGWCG 3 cut(s) 76, 248, 266
HpyAV CCTTC 5 cut(s) 211, 220, 297, 724, 802
HpyCH4III ACNGT 7 cut(s) 76, 433, 477, 508, 691, 910, 1019
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 7 cut(s) 491, 547, 628, 662, 694, 920, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 202, 245, 394, 926
HpyF3I CTNAG 3 cut(s) 318, 380, 1022
HpySE526I ACGT 1 cut(s) 404
Hsp92I GRCGYC 3 cut(s) 179, 246, 258
Hsp92II CATG 7 cut(s) 47, 330, 343, 348, 395, 666, 991
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 5 cut(s) 9, 297, 372, 376, 588
LmnI GCTCC 3 cut(s) 32, 239, 850
Lsp1109I GCAGC 4 cut(s) 524, 528, 531, 861
LweI GCATC 4 cut(s) 486, 615, 874, 965
MaeI CTAG 2 cut(s) 746, 770
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 175
MalI GATC 5 cut(s) 11, 299, 374, 378, 590
MboI GATC 5 cut(s) 9, 297, 372, 376, 588
MboII GAAGA 7 cut(s) 412, 434, 454, 617, 694, 697, 785
MfeI CAATTG 1 cut(s) 858
MhlI GDGCHC 4 cut(s) 37, 173, 236, 726
MlsI TGGCCA 1 cut(s) 325
MluCI AATT 5 cut(s) 331, 828, 858, 982, 1008
MluNI TGGCCA 1 cut(s) 325
MlyI GAGTC 1 cut(s) 708
MmeI TCCRAC 5 cut(s) 267, 476, 511, 566, 742
Mox20I TGGCCA 1 cut(s) 325
MroXI GAANNNNTTC 1 cut(s) 335
MscI TGGCCA 1 cut(s) 325
MseI TTAA 4 cut(s) 117, 285, 551, 894
MslI CAYNNNNRTG 2 cut(s) 161, 939
Msp20I TGGCCA 1 cut(s) 325
MspA1I CMGCKG 2 cut(s) 515, 1026
MspCI CTTAAG 2 cut(s) 550, 893
MspI CCGG 6 cut(s) 7, 30, 49, 80, 209, 754
MspR9I CCNGG 3 cut(s) 160, 522, 755
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 2 cut(s) 160, 522
MwoI GCNNNNNNNGC 4 cut(s) 202, 245, 394, 926
NciI CCSGG 1 cut(s) 755
NcoI CCATGG 3 cut(s) 43, 326, 344
NdeII GATC 5 cut(s) 9, 297, 372, 376, 588
NheI GCTAGC 1 cut(s) 745
NlaIII CATG 7 cut(s) 47, 330, 343, 348, 395, 666, 991
NlaIV GGNNCC 2 cut(s) 78, 485
NmuCI GTSAC 1 cut(s) 175
PctI GAATGC 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 1 cut(s) 913
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 8 cut(s) 16, 198, 201, 204, 514, 543, 546, 876
Ple19I CGATCG 1 cut(s) 375
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
PsiI TTATAA 1 cut(s) 1014
Psp124BI GAGCTC 3 cut(s) 37, 173, 236
Psp6I CCWGG 2 cut(s) 158, 520
PspGI CCWGG 2 cut(s) 158, 520
PspN4I GGNNCC 2 cut(s) 78, 485
PspPI GGNCC 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 848
PsyI GACNNNGTC 1 cut(s) 260
PvuI CGATCG 1 cut(s) 375
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 3 cut(s) 668, 803, 840
RsaNI GTAC 3 cut(s) 667, 802, 839
RseI CAYNNNNRTG 2 cut(s) 161, 939
SacI GAGCTC 3 cut(s) 37, 173, 236
SaqAI TTAA 4 cut(s) 117, 285, 551, 894
SatI GCNGC 8 cut(s) 15, 197, 200, 203, 513, 542, 545, 875
Sau3AI GATC 5 cut(s) 9, 297, 372, 376, 588
Sau96I GGNCC 1 cut(s) 220
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 1 cut(s) 708
ScrFI CCNGG 3 cut(s) 160, 522, 755
SduI GDGCHC 4 cut(s) 37, 173, 236, 726
SfaNI GCATC 4 cut(s) 486, 615, 874, 965
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 2 cut(s) 161, 939
SmlI CTYRAG 2 cut(s) 550, 893
SmoI CTYRAG 2 cut(s) 550, 893
Sse9I AATT 5 cut(s) 331, 828, 858, 982, 1008
SspMI CTAG 2 cut(s) 746, 770
SstI GAGCTC 3 cut(s) 37, 173, 236
StyD4I CCNGG 3 cut(s) 158, 520, 753
StyI CCWWGG 3 cut(s) 43, 326, 344
TaaI ACNGT 7 cut(s) 76, 433, 477, 508, 691, 910, 1019
TaiI ACGT 1 cut(s) 407
TaqI TCGA 4 cut(s) 71, 371, 375, 445
TasI AATT 5 cut(s) 331, 828, 858, 982, 1008
TatI WGTACW 1 cut(s) 801
TauI GCSGC 3 cut(s) 199, 202, 205
TfiI GAWTC 1 cut(s) 913
Tru1I TTAA 4 cut(s) 117, 285, 551, 894
Tru9I TTAA 4 cut(s) 117, 285, 551, 894
TscAI CASTG 2 cut(s) 694, 913
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 5 cut(s) 14, 512, 541, 544, 874
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 4 cut(s) 17, 328, 939, 968
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 2 cut(s) 694, 913
Tth111I GACNNNGTC 1 cut(s) 260
Vha464I CTTAAG 2 cut(s) 550, 893
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 1 cut(s) 1008
XbaI TCTAGA 1 cut(s) 769
XcmI CCANNNNNNNNNTGG 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 335
XspI CTAG 2 cut(s) 746, 770
ZrmI AGTACT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.