Rh4AG077300

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004710
Physical Location & Seq
Reverse (-)
15709916 .. 15739050
29135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG077300.1

Sequence Viewer

Length: 1005 bp
ATGAACCGGGTTGCGTTGAGGTCCGTAATCGGAGGTCTGCGCAGTGGCGGGGGCTGGAACCGGTACATTTCCACGGCGGCTGTTGCTAGGAATGTTGAGACAGCGGAGTTTCTGAAGCCGCAAAACGATGGCGTTCTTGGGGCGTTTCAGTGGAGGAGGATGATGAGCACGGGATCGGTTTCTGCTCAAACGTCGGAGGAGAAAGACCAGAAGGAGAGTGGAGCGAAGAAAGTGGATGATAGTATGGTGGTTTCGAATTACTGGGGGATTACGACGCCCAAGATTACTAGGGAGGATGGGACTGAGTGGCCATGGAATTGCTTTAGGCCATGGGAGAGTTATCAAGCGGACACCTCCATCGATCTGAACAAGCACCATCTGCCCAAGACGTTTCAGGACAAAATTGCGTTCAGGACTGTCAAGTTTCTTCGAGTTCTATCTGATCTTTACTTCAAGGAGCGGCATGGATGCCACGCAATGATGTTGGAAACTATCGCAGCTGTCCCTGGCATGGTGGGCGGAATGGTGCTACACTTAAGATCTCTCCGCAAGTTTGAGCATAGTGGTGGTTGGATCAAAGCTTTACTTGAAGAAGCAGAGAATGAGAGGATGCACCTTATGTCAGTAGTGGAACTTGTGAAGCCCGTGTGGCACGAGAGGCTACTGGTTCTGGCTGCGCAGGGGGTCTTCTTCAATGCCTTCTTTGTGCTCTATTTGCTTTCCCCCAAGGTGGCTCATAGGGTTACTGGGTATCTGGAGGAGGAAGCTGTAATCTCTTACACAGACTATTTGAAGGCTATCGAAAATGGCAAAATTGAAAATGTGCCGGCTCCTGCAATAGCCATTGACTACTGGAGGCTACCTAAAGATGCAACACTTCATGATGTTATTACTGTGATTCGTGCTGATGAAGCTCACCATAGGGATGTCAACCATTTTGCATCTGATATTCATTATCAGGGAAAGGAATTGAGAGAAGCCCCAGCTCCCGTTGGATATCACTAA

Protein Analysis

334

Amino Acids

37.93

Weight (kDa)

6.96

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 124 - 315 6.2e-82 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 41, 678
AccBSI CCGCTC 1 cut(s) 460
AciI CCGC 8 cut(s) 48, 77, 104, 119, 347, 460, 519, 547
AclWI GGATC 2 cut(s) 181, 581
AcoI YGGCCR 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 134
AcyI GRCGYC 1 cut(s) 275
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 2 cut(s) 511, 730
AflII CTTAAG 1 cut(s) 535
AgeI ACCGGT 1 cut(s) 60
AgsI TTSAA 5 cut(s) 454, 590, 694, 793, 818
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 5 cut(s) 500, 581, 767, 914, 986
AluI AGCT 5 cut(s) 500, 581, 767, 914, 986
Alw21I GWGCWC 2 cut(s) 170, 711
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 2 cut(s) 181, 581
AoxI GGCC 2 cut(s) 308, 326
ApeKI GCWGC 2 cut(s) 497, 674
AsiGI ACCGGT 1 cut(s) 60
AspLEI GCGC 2 cut(s) 42, 679
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 908
AsuII TTCGAA 1 cut(s) 254
AvaII GGWCC 1 cut(s) 21
BalI TGGCCA 1 cut(s) 310
BauI CACGAG 1 cut(s) 653
BbsI GAAGAC 1 cut(s) 679
Bbv12I GWGCWC 2 cut(s) 170, 711
BbvI GCAGC 2 cut(s) 509, 661
BccI CCATC 4 cut(s) 122, 290, 365, 384
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 507
BcnI CCSGG 1 cut(s) 8
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 2 cut(s) 87, 288
BfrI CTTAAG 1 cut(s) 535
BglI GCCNNNNNGGC 1 cut(s) 649
BglII AGATCT 1 cut(s) 539
BisI GCNGC 5 cut(s) 78, 119, 461, 498, 675
BlsI GCNGC 5 cut(s) 79, 120, 462, 499, 676
Bme1390I CCNGG 2 cut(s) 8, 507
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 2 cut(s) 59, 831
BmrFI CCNGG 2 cut(s) 8, 507
BmrI ACTGGG 2 cut(s) 271, 756
BmsI GCATC 4 cut(s) 458, 600, 859, 950
BmuI ACTGGG 2 cut(s) 271, 756
BpiI GAAGAC 1 cut(s) 679
BpmI CTGGAG 2 cut(s) 776, 874
Bpu14I TTCGAA 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 8
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 275
BsaJI CCNNGG 5 cut(s) 72, 311, 329, 505, 726
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 730
Bse118I RCCGGY 2 cut(s) 60, 826
Bse1I ACTGG 4 cut(s) 266, 669, 751, 857
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 1 cut(s) 507
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 5 cut(s) 72, 311, 329, 505, 726
BseGI GGATG 6 cut(s) 165, 241, 301, 473, 615, 931
BseLI CCNNNNNNNGG 2 cut(s) 511, 730
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 4 cut(s) 266, 669, 751, 857
BseRI GAGGAG 3 cut(s) 169, 212, 773
BseXI GCAGC 2 cut(s) 509, 661
BseYI CCCAGC 1 cut(s) 982
BshFI GGCC 2 cut(s) 310, 328
BshTI ACCGGT 1 cut(s) 60
BshVI ATCGAT 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 170, 711
BsiSI CCGG 3 cut(s) 7, 61, 827
BslFI GGGAC 2 cut(s) 313, 488
BslI CCNNNNNNNGG 2 cut(s) 511, 730
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 2 cut(s) 313, 488
BsnI GGCC 2 cut(s) 310, 328
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 170, 711
Bsp143I GATC 5 cut(s) 173, 361, 442, 539, 573
Bsp19I CCATGG 2 cut(s) 311, 329
BspACI CCGC 8 cut(s) 48, 77, 104, 119, 347, 460, 519, 547
BspANI GGCC 2 cut(s) 310, 328
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 1 cut(s) 360
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 2 cut(s) 59, 831
BspPI GGATC 2 cut(s) 181, 581
BspT104I TTCGAA 1 cut(s) 254
BspTI CTTAAG 1 cut(s) 535
BsrBI CCGCTC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 2 cut(s) 60, 826
BsrI ACTGG 4 cut(s) 266, 669, 751, 857
BssAI RCCGGY 2 cut(s) 60, 826
BssECI CCNNGG 5 cut(s) 72, 311, 329, 505, 726
BssMI GATC 5 cut(s) 173, 361, 442, 539, 573
BssNI GRCGYC 1 cut(s) 275
BssSI CACGAG 1 cut(s) 653
BssT1I CCWWGG 3 cut(s) 311, 329, 726
Bst2BI CACGAG 1 cut(s) 653
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 2 cut(s) 418, 895
BstACI GRCGYC 1 cut(s) 275
BstAFI CTTAAG 1 cut(s) 535
BstAPI GCANNNNNTGC 1 cut(s) 379
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 828
BstDEI CTNAG 1 cut(s) 303
BstDSI CCRYGG 3 cut(s) 72, 311, 329
BstF5I GGATG 6 cut(s) 165, 241, 301, 473, 615, 931
BstHHI GCGC 2 cut(s) 42, 679
BstKTI GATC 5 cut(s) 176, 364, 445, 542, 576
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 5 cut(s) 173, 361, 442, 539, 573
BstMWI GCNNNNNNNGC 7 cut(s) 83, 379, 516, 649, 658, 715, 911
BstNI CCWGG 1 cut(s) 507
BstSCI CCNGG 2 cut(s) 6, 505
BstV1I GCAGC 2 cut(s) 509, 661
BstV2I GAAGAC 1 cut(s) 679
BstX2I RGATCY 1 cut(s) 539
BstYI RGATCY 1 cut(s) 539
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 2 cut(s) 310, 328
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 3 cut(s) 72, 311, 329
BtsCI GGATG 6 cut(s) 165, 241, 301, 473, 615, 931
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 155
Cac8I GCNNGC 1 cut(s) 828
CciI TCATGA 1 cut(s) 880
CfoI GCGC 2 cut(s) 42, 679
Cfr10I RCCGGY 2 cut(s) 60, 826
Cfr13I GGNCC 1 cut(s) 21
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 64
CspAI ACCGGT 1 cut(s) 60
CviAII CATG 5 cut(s) 312, 330, 464, 511, 881
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 1 cut(s) 303
DpnI GATC 5 cut(s) 175, 363, 444, 541, 575
DpnII GATC 5 cut(s) 173, 361, 442, 539, 573
EaeI YGGCCR 1 cut(s) 308
EciI GGCGGA 1 cut(s) 534
Eco130I CCWWGG 3 cut(s) 311, 329, 726
Eco32I GATATC 1 cut(s) 998
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 505
EcoRV GATATC 1 cut(s) 998
EcoT14I CCWWGG 3 cut(s) 311, 329, 726
ErhI CCWWGG 3 cut(s) 311, 329, 726
FaeI CATG 5 cut(s) 315, 333, 467, 514, 884
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 2 cut(s) 313, 488
FatI CATG 5 cut(s) 311, 329, 463, 510, 880
FauI CCCGC 1 cut(s) 41
Fnu4HI GCNGC 5 cut(s) 78, 119, 461, 498, 675
FokI GGATG 6 cut(s) 172, 248, 308, 480, 622, 938
Fsp4HI GCNGC 5 cut(s) 78, 119, 461, 498, 675
FspBI CTAG 2 cut(s) 87, 288
FspI TGCGCA 2 cut(s) 41, 678
GlaI GCGC 2 cut(s) 41, 678
GluI GCNGC 5 cut(s) 78, 119, 461, 498, 675
GsaI CCCAGC 1 cut(s) 986
GsuI CTGGAG 2 cut(s) 776, 874
HaeIII GGCC 2 cut(s) 310, 328
HapII CCGG 3 cut(s) 7, 61, 827
HgaI GACGC 1 cut(s) 283
HhaI GCGC 2 cut(s) 42, 679
Hin1I GRCGYC 1 cut(s) 275
Hin1II CATG 5 cut(s) 315, 333, 467, 514, 884
Hin6I GCGC 2 cut(s) 40, 677
HinP1I GCGC 2 cut(s) 40, 677
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 1 cut(s) 898
HpaII CCGG 3 cut(s) 7, 61, 827
HphI GGTGA 1 cut(s) 908
Hpy166II GTNNAC 1 cut(s) 931
Hpy188I TCNGA 6 cut(s) 32, 114, 196, 366, 442, 946
Hpy188III TCNNGA 4 cut(s) 395, 412, 755, 881
Hpy8I GTNNAC 1 cut(s) 931
Hpy99I CGWCG 2 cut(s) 196, 277
HpyAV CCTTC 3 cut(s) 205, 709, 787
HpyCH4III ACNGT 2 cut(s) 418, 895
HpyCH4IV ACGT 2 cut(s) 191, 389
HpyCH4V TGCA 4 cut(s) 613, 836, 872, 941
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 379, 516, 649, 658, 715, 911
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 2 cut(s) 191, 389
Hsp92I GRCGYC 1 cut(s) 275
Hsp92II CATG 5 cut(s) 315, 333, 467, 514, 884
HspAI GCGC 2 cut(s) 40, 677
KroI GCCGGC 1 cut(s) 826
KroNI GCCGGC 1 cut(s) 828
Kzo9I GATC 5 cut(s) 173, 361, 442, 539, 573
LmnI GCTCC 4 cut(s) 221, 457, 835, 991
Lsp1109I GCAGC 2 cut(s) 509, 661
LweI GCATC 4 cut(s) 458, 600, 859, 950
MaeI CTAG 2 cut(s) 87, 288
MaeII ACGT 2 cut(s) 191, 389
MaeIII GTNAC 1 cut(s) 742
MalI GATC 5 cut(s) 175, 363, 444, 541, 575
MbiI CCGCTC 1 cut(s) 460
MboI GATC 5 cut(s) 173, 361, 442, 539, 573
MboII GAAGA 5 cut(s) 238, 419, 602, 679, 682
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 2 cut(s) 170, 711
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 5 cut(s) 256, 316, 402, 813, 968
MluNI TGGCCA 1 cut(s) 310
MmeI TCCRAC 4 cut(s) 174, 465, 551, 973
Mox20I TGGCCA 1 cut(s) 310
MroNI GCCGGC 1 cut(s) 826
MscI TGGCCA 1 cut(s) 310
MseI TTAA 1 cut(s) 536
MslI CAYNNNNRTG 2 cut(s) 564, 924
Msp20I TGGCCA 1 cut(s) 310
MspA1I CMGCKG 2 cut(s) 104, 500
MspCI CTTAAG 1 cut(s) 535
MspI CCGG 3 cut(s) 7, 61, 827
MspR9I CCNGG 2 cut(s) 8, 507
MvaI CCWGG 1 cut(s) 507
MwoI GCNNNNNNNGC 7 cut(s) 83, 379, 516, 649, 658, 715, 911
NaeI GCCGGC 1 cut(s) 828
NciI CCSGG 1 cut(s) 8
NcoI CCATGG 2 cut(s) 311, 329
NdeII GATC 5 cut(s) 173, 361, 442, 539, 573
NgoMIV GCCGGC 1 cut(s) 826
NlaIII CATG 5 cut(s) 315, 333, 467, 514, 884
NlaIV GGNNCC 2 cut(s) 59, 831
NsbI TGCGCA 2 cut(s) 41, 678
NspV TTCGAA 1 cut(s) 254
PagI TCATGA 1 cut(s) 880
PdiI GCCGGC 1 cut(s) 828
PfeI GAWTC 1 cut(s) 898
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 5 cut(s) 79, 120, 462, 499, 676
Psp6I CCWGG 1 cut(s) 505
PspFI CCCAGC 1 cut(s) 982
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 2 cut(s) 59, 831
PspPI GGNCC 1 cut(s) 21
PsuI RGATCY 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
RseI CAYNNNNRTG 2 cut(s) 564, 924
SaqAI TTAA 1 cut(s) 536
SatI GCNGC 5 cut(s) 78, 119, 461, 498, 675
Sau3AI GATC 5 cut(s) 173, 361, 442, 539, 573
Sau96I GGNCC 1 cut(s) 21
ScrFI CCNGG 2 cut(s) 8, 507
SduI GDGCHC 2 cut(s) 170, 711
SfaNI GCATC 4 cut(s) 458, 600, 859, 950
SfuI TTCGAA 1 cut(s) 254
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 2 cut(s) 564, 924
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
Sse9I AATT 5 cut(s) 256, 316, 402, 813, 968
SsiI CCGC 8 cut(s) 48, 77, 104, 119, 347, 460, 519, 547
SspMI CTAG 2 cut(s) 87, 288
StyD4I CCNGG 2 cut(s) 6, 505
StyI CCWWGG 3 cut(s) 311, 329, 726
TaaI ACNGT 2 cut(s) 418, 895
TaiI ACGT 2 cut(s) 194, 392
TaqI TCGA 4 cut(s) 254, 360, 430, 801
TasI AATT 5 cut(s) 256, 316, 402, 813, 968
TauI GCSGC 3 cut(s) 80, 121, 463
TfiI GAWTC 1 cut(s) 898
Tru1I TTAA 1 cut(s) 536
Tru9I TTAA 1 cut(s) 536
TscAI CASTG 2 cut(s) 49, 155
TseI GCWGC 2 cut(s) 497, 674
TspDTI ATGAA 4 cut(s) 17, 869, 924, 941
TspGWI ACGGA 1 cut(s) 13
TspRI CASTG 2 cut(s) 49, 155
Vha464I CTTAAG 1 cut(s) 535
VpaK11BI GGWCC 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XspI CTAG 2 cut(s) 87, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.