FvH4_4g05930

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004710
Physical Location & Seq
Reverse (-)
5356169 .. 5358182
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05930.t1

Sequence Viewer

Length: 975 bp
ATGAATAGGGTTGCGTTGAGGTCCGTGATCGGAGGTCTGCGCAGTGGCCGGAGCTGGAACCAGTACATTTCTCCTGCTGCAGCTAGGAATGTTGAGAGTGCGGAGTTTCAGTGGAGGAAGATGATGAGCACGGGGTCGGCTTCAGCTGAGAAAGAGGAGAAGGAGAGTGAAGTGAAGAAAGTGGAGGATAGCACGGTGGTTTCGAATTACTGGGGGATTATGAGGCCCAAGATTACTAGGGAGGATGGGACTGAGTGGCCGTGGAATTGCTTCAAGCCATGGGATACTTATCGAGCAGACACGTCCATCGATCTGAGCAAGCACCATGTGCCCAAGAATTTTCAGGACAGAGTTGCCTTCAGGGCTGTCAAGTTTCTTCGAGTTCTATCAGATCTTTACTTCAAGGAGCGGCATGGATGCCACGCAATGATGTTGGAAACTGTCGCAGGTGTCCCTGGCATGGTGGGAGGAATGGTGCTGCACTTAAGATCTCTCCGGAGGTTCGAGCACAGTGGTGGTTGGATCAAAGCTTTGCTTGAAGAAGCAGAGAATGAGAGGATGCACCTTATGACAGTAGGGGAACTCGTGAAGCCCGTGTGGCACGAGAGACTACTGGTTCTGGCTGCGCAGGGAGTGTTCTTCAATGCCTTCTTTGTGTTCTATTTGCTTTCCCCCAAGATTGCACATAGATTTACTGGGTACCTGGAGGAGGAAGCTGTAATATCTTACACAGATTATTTGAAGGCTATTGAAGATGGCAAAATTGAAAATGTGCCGGCTCCTGCTATAGCCATCGACTACTGGAGGCTACCTAAAGATGCAAGACTTCTTGATGTTATTACTGTGATTCGTGCTGATGAAGCTCACCATCGTGATGTCAACCATTTCGCATCTCTGTCTGTGTTTCAAATGCAGGATATTCAATTTCAGGGAAAAGAACTGAGAGAAGCACCGGCTCCCATTGGTTATCACTAA

Protein Analysis

325

Amino Acids

37.24

Weight (kDa)

7.25

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 107 - 296 2.2e-82 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 437
Acc16I TGCGCA 2 cut(s) 41, 627
Acc36I ACCTGC 1 cut(s) 437
Acc65I GGTACC 1 cut(s) 699
AccB1I GGYRCC 1 cut(s) 699
AccBSI CCGCTC 1 cut(s) 409
AccIII TCCGGA 1 cut(s) 495
AciI CCGC 2 cut(s) 101, 409
AclWI GGATC 1 cut(s) 530
AcoI YGGCCR 2 cut(s) 46, 257
AcsI RAATTY 1 cut(s) 337
AcuI CTGAAG 2 cut(s) 126, 343
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 2 cut(s) 65, 701
AfiI CCNNNNNNNGG 2 cut(s) 460, 709
AflII CTTAAG 1 cut(s) 484
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 9 cut(s) 274, 403, 539, 643, 742, 752, 767, 908, 923
AjiI CACGTC 1 cut(s) 303
AjnI CCWGG 2 cut(s) 454, 702
AleI CACNNNNGTG 2 cut(s) 513, 870
AluBI AGCT 6 cut(s) 54, 83, 146, 530, 716, 863
AluI AGCT 6 cut(s) 54, 83, 146, 530, 716, 863
Alw21I GWGCWC 2 cut(s) 131, 510
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 1 cut(s) 530
Aor13HI TCCGGA 1 cut(s) 495
AoxI GGCC 3 cut(s) 46, 224, 257
ApeKI GCWGC 4 cut(s) 77, 80, 478, 623
ApoI RAATTY 1 cut(s) 337
Asp700I GAANNNNTTC 1 cut(s) 269
Asp718I GGTACC 1 cut(s) 699
AspLEI GCGC 2 cut(s) 42, 628
AspS9I GGNCC 2 cut(s) 21, 225
AsuHPI GGTGA 1 cut(s) 857
AsuII TTCGAA 1 cut(s) 203
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 333
BanI GGYRCC 1 cut(s) 699
BauI CACGAG 2 cut(s) 584, 602
Bbv12I GWGCWC 2 cut(s) 131, 510
BbvI GCAGC 4 cut(s) 64, 92, 465, 610
BccI CCATC 5 cut(s) 239, 314, 749, 800, 876
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 2 cut(s) 456, 704
BciVI GTATCC 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 2 cut(s) 84, 237
BfmI CTRYAG 2 cut(s) 78, 786
BfrI CTTAAG 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 437
BfuI GTATCC 1 cut(s) 277
BglI GCCNNNNNGGC 2 cut(s) 362, 598
BglII AGATCT 2 cut(s) 391, 488
BisI GCNGC 5 cut(s) 78, 81, 410, 479, 624
BlsI GCNGC 5 cut(s) 79, 82, 411, 480, 625
Bme1390I CCNGG 2 cut(s) 456, 704
Bme18I GGWCC 1 cut(s) 21
BmgBI CACGTC 1 cut(s) 303
BmgT120I GGNCC 2 cut(s) 21, 225
BmiI GGNNCC 4 cut(s) 59, 701, 780, 957
BmrFI CCNGG 2 cut(s) 456, 704
BmrI ACTGGG 2 cut(s) 220, 705
BmsI GCATC 4 cut(s) 407, 549, 808, 899
BmuI ACTGGG 2 cut(s) 220, 705
BpmI CTGGAG 2 cut(s) 725, 823
Bpu14I TTCGAA 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 3 cut(s) 260, 278, 454
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 459, 489
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 709
Bse118I RCCGGY 2 cut(s) 775, 952
Bse1I ACTGG 5 cut(s) 61, 215, 618, 700, 806
Bse3DI GCAATG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 288
BseAI TCCGGA 1 cut(s) 495
BseBI CCWGG 2 cut(s) 456, 704
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 3 cut(s) 260, 278, 454
BseGI GGATG 3 cut(s) 250, 422, 564
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 460, 709
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 4 cut(s) 138, 243, 305, 932
BseNI ACTGG 5 cut(s) 61, 215, 618, 700, 806
BseRI GAGGAG 2 cut(s) 170, 722
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 4 cut(s) 64, 92, 465, 610
BsgI GTGCAG 1 cut(s) 464
BshFI GGCC 3 cut(s) 48, 226, 259
BshNI GGYRCC 1 cut(s) 699
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 2 cut(s) 131, 510
BsiSI CCGG 4 cut(s) 49, 496, 776, 953
BslFI GGGAC 2 cut(s) 262, 437
BslI CCNNNNNNNGG 2 cut(s) 460, 709
BsmAI GTCTC 1 cut(s) 601
BsmFI GGGAC 2 cut(s) 262, 437
BsnI GGCC 3 cut(s) 48, 226, 259
Bsp119I TTCGAA 1 cut(s) 203
Bsp1286I GDGCHC 3 cut(s) 131, 333, 510
Bsp13I TCCGGA 1 cut(s) 495
Bsp143I GATC 5 cut(s) 27, 310, 391, 488, 522
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 2 cut(s) 101, 409
BspANI GGCC 3 cut(s) 48, 226, 259
BspCNI CTCAG 4 cut(s) 139, 244, 306, 933
BspDI ATCGAT 1 cut(s) 309
BspEI TCCGGA 1 cut(s) 495
BspLI GGNNCC 4 cut(s) 59, 701, 780, 957
BspMAI CTGCAG 1 cut(s) 82
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 1 cut(s) 530
BspT104I TTCGAA 1 cut(s) 203
BspT107I GGYRCC 1 cut(s) 699
BspTI CTTAAG 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 2 cut(s) 775, 952
BsrI ACTGG 5 cut(s) 61, 215, 618, 700, 806
BssAI RCCGGY 2 cut(s) 775, 952
BssECI CCNNGG 3 cut(s) 260, 278, 454
BssMI GATC 5 cut(s) 27, 310, 391, 488, 522
BssSI CACGAG 2 cut(s) 584, 602
BssT1I CCWWGG 1 cut(s) 278
Bst2BI CACGAG 2 cut(s) 584, 602
Bst2UI CCWGG 2 cut(s) 456, 704
Bst4CI ACNGT 5 cut(s) 196, 442, 512, 574, 844
BstAFI CTTAAG 1 cut(s) 484
BstAPI GCANNNNNTGC 1 cut(s) 328
BstBI TTCGAA 1 cut(s) 203
BstC8I GCNNGC 2 cut(s) 320, 777
BstDEI CTNAG 4 cut(s) 147, 252, 314, 941
BstDSI CCRYGG 2 cut(s) 260, 278
BstENI CCTNNNNNAGG 1 cut(s) 707
BstF5I GGATG 3 cut(s) 250, 422, 564
BstHHI GCGC 2 cut(s) 42, 628
BstKTI GATC 5 cut(s) 30, 313, 394, 491, 525
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 5 cut(s) 27, 310, 391, 488, 522
BstMWI GCNNNNNNNGC 4 cut(s) 328, 362, 598, 860
BstNI CCWGG 2 cut(s) 456, 704
BstSCI CCNGG 2 cut(s) 454, 702
BstSFI CTRYAG 2 cut(s) 78, 786
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 64, 92, 465, 610
BstX2I RGATCY 2 cut(s) 391, 488
BstYI RGATCY 2 cut(s) 391, 488
Bsu15I ATCGAT 1 cut(s) 309
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 3 cut(s) 48, 226, 259
BsuTUI ATCGAT 1 cut(s) 309
BtgI CCRYGG 2 cut(s) 260, 278
BtrI CACGTC 1 cut(s) 303
BtsCI GGATG 3 cut(s) 250, 422, 564
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 3 cut(s) 49, 116, 517
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 2 cut(s) 320, 777
CfoI GCGC 2 cut(s) 42, 628
Cfr10I RCCGGY 2 cut(s) 775, 952
Cfr13I GGNCC 2 cut(s) 21, 225
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 2 cut(s) 64, 700
CviAII CATG 4 cut(s) 279, 326, 413, 460
CviQI GTAC 2 cut(s) 64, 700
DdeI CTNAG 4 cut(s) 147, 252, 314, 941
DpnI GATC 5 cut(s) 29, 312, 393, 490, 524
DpnII GATC 5 cut(s) 27, 310, 391, 488, 522
DraIII CACNNNGTG 1 cut(s) 328
EaeI YGGCCR 2 cut(s) 46, 257
Eco130I CCWWGG 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 2 cut(s) 126, 343
EcoNI CCTNNNNNAGG 1 cut(s) 707
EcoRII CCWGG 2 cut(s) 454, 702
EcoT14I CCWWGG 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 4 cut(s) 282, 329, 416, 463
FaiI YATR 8 cut(s) 221, 280, 327, 414, 461, 569, 687, 788
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 2 cut(s) 262, 437
FatI CATG 4 cut(s) 278, 325, 412, 459
Fnu4HI GCNGC 5 cut(s) 78, 81, 410, 479, 624
FokI GGATG 3 cut(s) 257, 429, 571
Fsp4HI GCNGC 5 cut(s) 78, 81, 410, 479, 624
FspBI CTAG 2 cut(s) 84, 237
FspI TGCGCA 2 cut(s) 41, 627
GlaI GCGC 2 cut(s) 41, 627
GluI GCNGC 5 cut(s) 78, 81, 410, 479, 624
GsuI CTGGAG 2 cut(s) 725, 823
HaeIII GGCC 3 cut(s) 48, 226, 259
HapII CCGG 4 cut(s) 49, 496, 776, 953
HhaI GCGC 2 cut(s) 42, 628
Hin1II CATG 4 cut(s) 282, 329, 416, 463
Hin6I GCGC 2 cut(s) 40, 626
HinP1I GCGC 2 cut(s) 40, 626
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 1 cut(s) 847
HpaII CCGG 4 cut(s) 49, 496, 776, 953
HphI GGTGA 1 cut(s) 857
Hpy166II GTNNAC 1 cut(s) 880
Hpy188I TCNGA 3 cut(s) 32, 315, 391
Hpy188III TCNNGA 5 cut(s) 344, 496, 586, 830, 872
Hpy8I GTNNAC 1 cut(s) 880
HpyAV CCTTC 4 cut(s) 154, 367, 658, 736
HpyCH4III ACNGT 5 cut(s) 196, 442, 512, 574, 844
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 6 cut(s) 80, 481, 562, 683, 821, 913
HpyF10VI GCNNNNNNNGC 4 cut(s) 328, 362, 598, 860
HpyF3I CTNAG 4 cut(s) 147, 252, 314, 941
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 4 cut(s) 282, 329, 416, 463
HspAI GCGC 2 cut(s) 40, 626
Kpn2I TCCGGA 1 cut(s) 495
KpnI GGTACC 1 cut(s) 703
KroI GCCGGC 1 cut(s) 775
KroNI GCCGGC 1 cut(s) 777
Kzo9I GATC 5 cut(s) 27, 310, 391, 488, 522
LmnI GCTCC 4 cut(s) 51, 406, 784, 961
Lsp1109I GCAGC 4 cut(s) 64, 92, 465, 610
LweI GCATC 4 cut(s) 407, 549, 808, 899
MaeI CTAG 2 cut(s) 84, 237
MaeII ACGT 1 cut(s) 302
MalI GATC 5 cut(s) 29, 312, 393, 490, 524
MbiI CCGCTC 1 cut(s) 409
MboI GATC 5 cut(s) 27, 310, 391, 488, 522
MboII GAAGA 6 cut(s) 130, 187, 368, 551, 631, 764
MflI RGATCY 2 cut(s) 391, 488
MhlI GDGCHC 3 cut(s) 131, 333, 510
MluCI AATT 5 cut(s) 205, 265, 337, 762, 923
MmeI TCCRAC 2 cut(s) 414, 500
MroI TCCGGA 1 cut(s) 495
MroNI GCCGGC 1 cut(s) 775
MroXI GAANNNNTTC 1 cut(s) 269
MseI TTAA 1 cut(s) 485
MslI CAYNNNNRTG 3 cut(s) 513, 870, 873
MspA1I CMGCKG 1 cut(s) 146
MspCI CTTAAG 1 cut(s) 484
MspI CCGG 4 cut(s) 49, 496, 776, 953
MspR9I CCNGG 2 cut(s) 456, 704
MvaI CCWGG 2 cut(s) 456, 704
MwoI GCNNNNNNNGC 4 cut(s) 328, 362, 598, 860
NaeI GCCGGC 1 cut(s) 777
NcoI CCATGG 1 cut(s) 278
NdeII GATC 5 cut(s) 27, 310, 391, 488, 522
NgoMIV GCCGGC 1 cut(s) 775
NlaIII CATG 4 cut(s) 282, 329, 416, 463
NlaIV GGNNCC 4 cut(s) 59, 701, 780, 957
NsbI TGCGCA 2 cut(s) 41, 627
NspV TTCGAA 1 cut(s) 203
OliI CACNNNNGTG 2 cut(s) 513, 870
PaqCI CACCTGC 1 cut(s) 437
PcsI WCGNNNNNNNCGW 2 cut(s) 200, 591
PdiI GCCGGC 1 cut(s) 777
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 847
PkrI GCNGC 5 cut(s) 79, 82, 411, 480, 625
Psp6I CCWGG 2 cut(s) 454, 702
PspGI CCWGG 2 cut(s) 454, 702
PspN4I GGNNCC 4 cut(s) 59, 701, 780, 957
PspPI GGNCC 2 cut(s) 21, 225
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 391, 488
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 2 cut(s) 65, 701
RsaNI GTAC 2 cut(s) 64, 700
RseI CAYNNNNRTG 3 cut(s) 513, 870, 873
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 5 cut(s) 78, 81, 410, 479, 624
Sau3AI GATC 5 cut(s) 27, 310, 391, 488, 522
Sau96I GGNCC 2 cut(s) 21, 225
ScrFI CCNGG 2 cut(s) 456, 704
SduI GDGCHC 3 cut(s) 131, 333, 510
SfaNI GCATC 4 cut(s) 407, 549, 808, 899
SfcI CTRYAG 2 cut(s) 78, 786
SfuI TTCGAA 1 cut(s) 203
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 3 cut(s) 513, 870, 873
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
Sse9I AATT 5 cut(s) 205, 265, 337, 762, 923
SsiI CCGC 2 cut(s) 101, 409
SspMI CTAG 2 cut(s) 84, 237
StyD4I CCNGG 2 cut(s) 454, 702
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 5 cut(s) 196, 442, 512, 574, 844
TaiI ACGT 1 cut(s) 305
TaqI TCGA 6 cut(s) 203, 292, 309, 379, 504, 795
TasI AATT 5 cut(s) 205, 265, 337, 762, 923
TatI WGTACW 1 cut(s) 63
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 1 cut(s) 847
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 3 cut(s) 49, 116, 517
TseI GCWGC 4 cut(s) 77, 80, 478, 623
TspDTI ATGAA 2 cut(s) 17, 873
TspGWI ACGGA 1 cut(s) 13
TspRI CASTG 3 cut(s) 49, 116, 517
Vha464I CTTAAG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 21
XagI CCTNNNNNAGG 1 cut(s) 707
XapI RAATTY 1 cut(s) 337
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 2 cut(s) 84, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.