MD16G1236400.v1.1

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004710
Physical Location & Seq
Reverse (-)
24847685 .. 24850064
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1236400.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGAACAGGATCGCAGTGAGGTCGTTGGTCGGCGGTCTGCGCGGTGCCCGGAACTACAACCGGTACATTTCCACGGCGGTCGCTGCAAGGCCCTTGGACAGCGCGGAGTTCCTGAACCGTCGGAGCGGCGACGGTTTTAGCGGATTTTACTGGATGAGGATGATGAGCAGCGCGCCGCAACCTGTTGCGCCGCCGGTTGGTGCTACGTCGGCGGAGAAGGAGCAGAAGGAGAGCGGGGGCTCGGTGGCCGACGGCGGCAAGAAAGGAAGCGACAGCAACGTCGTTGTGTCGAACTACTGGGGAATTCAGAGGCCGAAGATCACAAGGGAGGACGGGACTGAGTGGCCTTGGAATTGCTTCATGCCATGGGAAACCTATAGGGCGGACCTATCCATCGATCTGGGCAAGCACCATGTGCCCAAGACTTTTCTAGACAGATTTGCCTTCAGGACCGTCAAGCTTCTTCGGATTCCAACGGATATTTTCTTTCAGAGACGGTATGGGTGCCGTGCAATGATGTTGGAAACTGTGGCAGCTGTCCCTGGCATGGTCGGAGGAATGCTGCTGCACTTAAGGTCTCTGCGCAAGTTTCAGCATAGTGGTGGTTGGATCAAGGCTTTACTTGAAGAGGCAGAGAACGAGAGAATGCACCTGATGACAATGGTTGAACTTGTGAAGCCCGTGTGGTACGAGAGACTGCTGGTTCTCGCTGTGCAGGGAGTCTTCTTCAATGCCTTCTTTGTTCTTTATATGATTTCCCCAAAACTGGCACATAGGATTGTTGGGTATCTGGAGGAGGAGGCTATACACTCTTATACAGAGTATTTGAAGGATATCGATAGTGGCAAGATTGAAAACGTACAGGCTCCTGCAATTGCTATTGACTACTGGAGGTTACCTAAAGATGCGAAGCTCAAGGATGTCATTACTGTGATTCGTGCTGATGAAGCTCATCATCGTGATGTCAACCATTTTGCATCTGACATTCATTACGAGGGCAAGGAATTGCGAGAAGCACCGGCTCCCCTTGGTTATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AOX

Protein Analysis

347

Amino Acids

39.35

Weight (kDa)

9.01

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 136 - 327 9.1e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
Acc16I TGCGCA 1 cut(s) 584
AccB1I GGYRCC 2 cut(s) 44, 504
AccBSI CCGCTC 2 cut(s) 126, 234
AccII CGCG 3 cut(s) 42, 104, 173
AclWI GGATC 2 cut(s) 17, 617
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 303
AcuI CTGAAG 1 cut(s) 430
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 3 cut(s) 65, 689, 861
AfiI CCNNNNNNNGG 3 cut(s) 197, 547, 766
AflII CTTAAG 1 cut(s) 571
AgeI ACCGGT 1 cut(s) 60
AgsI TTSAA 5 cut(s) 626, 668, 730, 829, 854
AjnI CCWGG 1 cut(s) 541
AluBI AGCT 4 cut(s) 460, 536, 913, 950
AluI AGCT 4 cut(s) 460, 536, 913, 950
Alw26I GTCTC 3 cut(s) 487, 582, 688
AlwI GGATC 2 cut(s) 17, 617
AoxI GGCC 4 cut(s) 89, 246, 311, 344
ApeKI GCWGC 5 cut(s) 83, 168, 533, 562, 565
ApoI RAATTY 1 cut(s) 303
AsiGI ACCGGT 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 356
AspLEI GCGC 6 cut(s) 42, 104, 173, 175, 190, 585
AspS9I GGNCC 3 cut(s) 90, 385, 450
AsuC2I CCSGG 1 cut(s) 49
AvaII GGWCC 2 cut(s) 385, 450
BaeGI GKGCMC 2 cut(s) 49, 420
BanI GGYRCC 2 cut(s) 44, 504
BanII GRGCYC 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 715
BbvI GCAGC 5 cut(s) 70, 180, 545, 549, 552
BccI CCATC 1 cut(s) 401
BceAI ACGGC 3 cut(s) 90, 268, 492
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 543
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 3 cut(s) 487, 582, 688
BfaI CTAG 1 cut(s) 431
BfmI CTRYAG 1 cut(s) 376
BfrI CTTAAG 1 cut(s) 571
BisI GCNGC 9 cut(s) 84, 127, 169, 176, 191, 256, 534, 563, 566
BlsI GCNGC 9 cut(s) 85, 128, 170, 177, 192, 257, 535, 564, 567
Bme1390I CCNGG 2 cut(s) 49, 543
Bme18I GGWCC 2 cut(s) 385, 450
BmgT120I GGNCC 3 cut(s) 90, 385, 450
BmiI GGNNCC 4 cut(s) 46, 506, 867, 1023
BmrFI CCNGG 2 cut(s) 49, 543
BmrI ACTGGG 1 cut(s) 307
BmsI GCATC 2 cut(s) 895, 986
BmuI ACTGGG 1 cut(s) 307
BpiI GAAGAC 1 cut(s) 715
BpmI CTGGAG 2 cut(s) 812, 910
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 49
Bsa29I ATCGAT 2 cut(s) 396, 837
BsaI GGTCTC 1 cut(s) 582
BsaJI CCNNGG 6 cut(s) 72, 93, 347, 365, 541, 1027
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 547, 766
Bse118I RCCGGY 3 cut(s) 60, 193, 1018
Bse1I ACTGG 4 cut(s) 155, 302, 771, 893
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 543
BseCI ATCGAT 2 cut(s) 396, 837
BseDI CCNNGG 6 cut(s) 72, 93, 347, 365, 541, 1027
BseGI GGATG 3 cut(s) 159, 165, 925
BseLI CCNNNNNNNGG 3 cut(s) 197, 547, 766
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 4 cut(s) 155, 302, 771, 893
BsePI GCGCGC 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 809, 812
BseSI GKGCMC 2 cut(s) 49, 420
BseXI GCAGC 5 cut(s) 70, 180, 545, 549, 552
BsgI GTGCAG 2 cut(s) 551, 734
Bsh1236I CGCG 3 cut(s) 42, 104, 173
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 4 cut(s) 91, 248, 313, 346
BshNI GGYRCC 2 cut(s) 44, 504
BshTI ACCGGT 1 cut(s) 60
BshVI ATCGAT 2 cut(s) 396, 837
BsiEI CGRYCG 1 cut(s) 81
BsiSI CCGG 4 cut(s) 49, 61, 194, 1019
BslFI GGGAC 2 cut(s) 349, 524
BslI CCNNNNNNNGG 3 cut(s) 197, 547, 766
BsmAI GTCTC 3 cut(s) 487, 582, 688
BsmBI CGTCTC 1 cut(s) 487
BsmFI GGGAC 2 cut(s) 349, 524
BsmI GAATGC 2 cut(s) 564, 651
BsnI GGCC 4 cut(s) 91, 248, 313, 346
Bso31I GGTCTC 1 cut(s) 582
Bsp1286I GDGCHC 3 cut(s) 49, 242, 420
Bsp143I GATC 4 cut(s) 9, 318, 397, 609
Bsp19I CCATGG 1 cut(s) 365
BspANI GGCC 4 cut(s) 91, 248, 313, 346
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 2 cut(s) 396, 837
BspFNI CGCG 3 cut(s) 42, 104, 173
BspLI GGNNCC 4 cut(s) 46, 506, 867, 1023
BspPI GGATC 2 cut(s) 17, 617
BspT107I GGYRCC 2 cut(s) 44, 504
BspTI CTTAAG 1 cut(s) 571
BspTNI GGTCTC 1 cut(s) 582
BsrBI CCGCTC 2 cut(s) 126, 234
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 3 cut(s) 60, 193, 1018
BsrI ACTGG 4 cut(s) 155, 302, 771, 893
BssAI RCCGGY 3 cut(s) 60, 193, 1018
BssECI CCNNGG 6 cut(s) 72, 93, 347, 365, 541, 1027
BssHII GCGCGC 1 cut(s) 171
BssMI GATC 4 cut(s) 9, 318, 397, 609
BssT1I CCWWGG 4 cut(s) 93, 347, 365, 1027
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 6 cut(s) 119, 134, 454, 498, 529, 931
Bst6I CTCTTC 1 cut(s) 621
BstAFI CTTAAG 1 cut(s) 571
BstAPI GCANNNNNTGC 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 173, 407
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 2 cut(s) 72, 365
BstEII GGTNACC 1 cut(s) 894
BstF5I GGATG 3 cut(s) 159, 165, 925
BstFNI CGCG 3 cut(s) 42, 104, 173
BstHHI GCGC 6 cut(s) 42, 104, 173, 175, 190, 585
BstKTI GATC 4 cut(s) 12, 321, 400, 612
BstMAI GTCTC 3 cut(s) 487, 582, 688
BstMBI GATC 4 cut(s) 9, 318, 397, 609
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 5 cut(s) 39, 83, 209, 415, 947
BstNI CCWGG 1 cut(s) 543
BstPI GGTNACC 1 cut(s) 894
BstSCI CCNGG 2 cut(s) 47, 541
BstSFI CTRYAG 1 cut(s) 376
BstSLI GKGCMC 2 cut(s) 49, 420
BstUI CGCG 3 cut(s) 42, 104, 173
BstV1I GCAGC 5 cut(s) 70, 180, 545, 549, 552
BstV2I GAAGAC 1 cut(s) 715
BstXI CCANNNNNNTGG 1 cut(s) 400
Bsu15I ATCGAT 2 cut(s) 396, 837
BsuRI GGCC 4 cut(s) 91, 248, 313, 346
BsuTUI ATCGAT 2 cut(s) 396, 837
BtgI CCRYGG 2 cut(s) 72, 365
BtsCI GGATG 3 cut(s) 159, 165, 925
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 21, 1036
Cac8I GCNNGC 2 cut(s) 173, 407
CfoI GCGC 6 cut(s) 42, 104, 173, 175, 190, 585
Cfr10I RCCGGY 3 cut(s) 60, 193, 1018
Cfr13I GGNCC 3 cut(s) 90, 385, 450
ClaI ATCGAT 2 cut(s) 396, 837
Csp6I GTAC 3 cut(s) 64, 688, 860
CspAI ACCGGT 1 cut(s) 60
CviAII CATG 4 cut(s) 361, 366, 413, 547
CviQI GTAC 3 cut(s) 64, 688, 860
DdeI CTNAG 1 cut(s) 339
DpnI GATC 4 cut(s) 11, 320, 399, 611
DpnII GATC 4 cut(s) 9, 318, 397, 609
DraIII CACNNNGTG 1 cut(s) 415
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
EciI GGCGGA 2 cut(s) 227, 398
Eco130I CCWWGG 4 cut(s) 93, 347, 365, 1027
Eco24I GRGCYC 1 cut(s) 242
Eco31I GGTCTC 1 cut(s) 582
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 2 cut(s) 385, 450
Eco57I CTGAAG 1 cut(s) 430
Eco91I GGTNACC 1 cut(s) 894
EcoO109I RGGNCCY 1 cut(s) 90
EcoO65I GGTNACC 1 cut(s) 894
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 541
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 4 cut(s) 93, 347, 365, 1027
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 4 cut(s) 93, 347, 365, 1027
Esp3I CGTCTC 1 cut(s) 487
FaeI CATG 4 cut(s) 364, 369, 416, 550
FaqI GGGAC 2 cut(s) 349, 524
FatI CATG 4 cut(s) 360, 365, 412, 546
FauI CCCGC 1 cut(s) 227
Fnu4HI GCNGC 9 cut(s) 84, 127, 169, 176, 191, 256, 534, 563, 566
FokI GGATG 3 cut(s) 166, 172, 932
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 9 cut(s) 84, 127, 169, 176, 191, 256, 534, 563, 566
FspBI CTAG 1 cut(s) 431
FspI TGCGCA 1 cut(s) 584
GlaI GCGC 6 cut(s) 41, 103, 172, 174, 189, 584
GluI GCNGC 9 cut(s) 84, 127, 169, 176, 191, 256, 534, 563, 566
GsuI CTGGAG 2 cut(s) 812, 910
HaeIII GGCC 4 cut(s) 91, 248, 313, 346
HapII CCGG 4 cut(s) 49, 61, 194, 1019
HhaI GCGC 6 cut(s) 42, 104, 173, 175, 190, 585
Hin1II CATG 4 cut(s) 364, 369, 416, 550
Hin6I GCGC 6 cut(s) 40, 102, 171, 173, 188, 583
HinP1I GCGC 6 cut(s) 40, 102, 171, 173, 188, 583
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 3 cut(s) 469, 720, 934
HpaII CCGG 4 cut(s) 49, 61, 194, 1019
Hpy166II GTNNAC 1 cut(s) 967
Hpy188I TCNGA 6 cut(s) 123, 309, 468, 492, 554, 982
Hpy188III TCNNGA 5 cut(s) 112, 431, 448, 791, 959
Hpy8I GTNNAC 1 cut(s) 967
Hpy99I CGWCG 5 cut(s) 123, 134, 211, 254, 284
HpyAV CCTTC 5 cut(s) 211, 220, 454, 745, 823
HpyCH4III ACNGT 6 cut(s) 119, 134, 454, 498, 529, 931
HpyCH4IV ACGT 3 cut(s) 206, 279, 858
HpyCH4V TGCA 7 cut(s) 86, 512, 568, 649, 715, 872, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 83, 209, 415, 947
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 3 cut(s) 206, 279, 858
Hsp92II CATG 4 cut(s) 364, 369, 416, 550
HspAI GCGC 6 cut(s) 40, 102, 171, 173, 188, 583
Kzo9I GATC 4 cut(s) 9, 318, 397, 609
LmnI GCTCC 4 cut(s) 123, 220, 871, 1027
Lsp1109I GCAGC 5 cut(s) 70, 180, 545, 549, 552
LweI GCATC 2 cut(s) 895, 986
MaeI CTAG 1 cut(s) 431
MaeII ACGT 3 cut(s) 206, 279, 858
MaeIII GTNAC 1 cut(s) 894
MalI GATC 4 cut(s) 11, 320, 399, 611
MbiI CCGCTC 2 cut(s) 126, 234
MboI GATC 4 cut(s) 9, 318, 397, 609
MboII GAAGA 5 cut(s) 328, 455, 638, 715, 718
MfeI CAATTG 1 cut(s) 873
MhlI GDGCHC 3 cut(s) 49, 242, 420
MluCI AATT 4 cut(s) 303, 352, 873, 1004
MlyI GAGTC 1 cut(s) 729
MmeI TCCRAC 5 cut(s) 101, 497, 501, 532, 587
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 1 cut(s) 572
MslI CAYNNNNRTG 4 cut(s) 600, 929, 957, 960
MspA1I CMGCKG 1 cut(s) 536
MspCI CTTAAG 1 cut(s) 571
MspI CCGG 4 cut(s) 49, 61, 194, 1019
MspR9I CCNGG 2 cut(s) 49, 543
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 2 cut(s) 564, 651
MvaI CCWGG 1 cut(s) 543
MvnI CGCG 3 cut(s) 42, 104, 173
MwoI GCNNNNNNNGC 5 cut(s) 39, 83, 209, 415, 947
NciI CCSGG 1 cut(s) 49
NcoI CCATGG 1 cut(s) 365
NdeII GATC 4 cut(s) 9, 318, 397, 609
NlaIII CATG 4 cut(s) 364, 369, 416, 550
NlaIV GGNNCC 4 cut(s) 46, 506, 867, 1023
NsbI TGCGCA 1 cut(s) 584
PauI GCGCGC 1 cut(s) 171
PctI GAATGC 2 cut(s) 564, 651
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 469, 934
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 9 cut(s) 85, 128, 170, 177, 192, 257, 535, 564, 567
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
Psp6I CCWGG 1 cut(s) 541
PspEI GGTNACC 1 cut(s) 894
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 4 cut(s) 46, 506, 867, 1023
PspPI GGNCC 3 cut(s) 90, 385, 450
PteI GCGCGC 1 cut(s) 171
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 3 cut(s) 65, 689, 861
RsaNI GTAC 3 cut(s) 64, 688, 860
RseI CAYNNNNRTG 4 cut(s) 600, 929, 957, 960
SaqAI TTAA 1 cut(s) 572
SatI GCNGC 9 cut(s) 84, 127, 169, 176, 191, 256, 534, 563, 566
Sau3AI GATC 4 cut(s) 9, 318, 397, 609
Sau96I GGNCC 3 cut(s) 90, 385, 450
SchI GAGTC 1 cut(s) 729
ScrFI CCNGG 2 cut(s) 49, 543
SduI GDGCHC 3 cut(s) 49, 242, 420
SfaNI GCATC 2 cut(s) 895, 986
SfcI CTRYAG 1 cut(s) 376
SinI GGWCC 2 cut(s) 385, 450
SmiMI CAYNNNNRTG 4 cut(s) 600, 929, 957, 960
SmlI CTYRAG 2 cut(s) 571, 914
SmoI CTYRAG 2 cut(s) 571, 914
Sse9I AATT 4 cut(s) 303, 352, 873, 1004
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 47, 541
StyI CCWWGG 4 cut(s) 93, 347, 365, 1027
TaaI ACNGT 6 cut(s) 119, 134, 454, 498, 529, 931
TaiI ACGT 3 cut(s) 209, 282, 861
TaqI TCGA 3 cut(s) 290, 396, 837
TasI AATT 4 cut(s) 303, 352, 873, 1004
TauI GCSGC 4 cut(s) 129, 178, 193, 258
TfiI GAWTC 2 cut(s) 469, 934
Tru1I TTAA 1 cut(s) 572
Tru9I TTAA 1 cut(s) 572
TscAI CASTG 1 cut(s) 21
TseI GCWGC 5 cut(s) 83, 168, 533, 562, 565
TspDTI ATGAA 4 cut(s) 17, 349, 960, 977
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 1 cut(s) 21
Vha464I CTTAAG 1 cut(s) 571
VpaK11BI GGWCC 2 cut(s) 385, 450
XapI RAATTY 1 cut(s) 303
XbaI TCTAGA 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.