Rw4G006240

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004710
Physical Location & Seq
Reverse (-)
12772862 .. 12775328
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006240.1

Sequence Viewer

Length: 1017 bp
ATGAACCGGATCGCGGCGAGGTCGGTGGCCGGAGCTCTGCGCCATGGCCGGAACTTCAACCGCCACATTTCGACCACGCCGGTCGCTGCAAGGACTTTGGACACCGCCGATTATCTGAACCGCCAGAACGCCACCGCTTTCGGCTCATTTTACTCCAGCAGGATGATGAGCTCGGTGACGCCATCCGTCCAAACGCCGCCGCCTCCGGCGGAGAAGGACCAGAAGGAGAGCTCCGCCTCCGACGCCAAGAAAGACGCCGTCGTTTCACATTATTGGGGGATTAAGAGGCCAGAGATCAAAAGGGAGGACGGCTCTGAGTGGCCATGGAATTGCTTCATGCCATGGGAGACTTATCAAGCAGATTTATCGATCGATCTGAGCAAGCACCATGTGCCGAAGACGTTTCTGGACAAAGTGGCGTACAGAACAGTCAAGCTTCTTCGAATTCCGACTGATATCTTCTTTCAGAGACGGTATGGGTGCCGTGCAATGATGCTGGAAACTGTGGCAGCTGTCCCTGGTATGGTTGGAGGAATGCTGCTGCACTTAAGGTCTCTCCGAAAGTTCCAGCAGAGTGGTGGTTGGATCAAAGCCTTACTTGAAGAAGCAGAGAATGAGAGGATGCACTTGATGACAATGGTGGAACTCGTGAAGCCTGCATGGTACGAGAGGCTACTGGTTCTCACTGTGCAGGGAGTCTTCTTCAATGCCTTCTTTGTGCTCTATATGATTTCCCCCAAGCTAGCACACCGGGTTGTTGGATATCTAGAAGAGGAGGCCATTCACTCTTATACAGAGTACTTGAAGGATATTGATAGTGGCAAAATTGAAAATGTACCAGCTCCTGCTATAGCAATTGACTACTGGAGGCTGCCAAAAGATGCTACACTTAAGGATGTTATCACTGTGATTCGTGCAGATGAAGCTCACCATAGGGATGTCAACCATTTTGCATCTGATATTCATTATCAGGGAAAGGAATTGAGAGAAGCCCCAGCTCCCGTTGGATATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AOX

Protein Analysis

338

Amino Acids

38.59

Weight (kDa)

8.85

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 127 - 319 1.3e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 480
AccII CGCG 1 cut(s) 14
AciI CCGC 9 cut(s) 14, 61, 105, 121, 135, 197, 200, 209, 234
AclWI GGATC 2 cut(s) 17, 593
AcoI YGGCCR 3 cut(s) 27, 46, 320
AcsI RAATTY 1 cut(s) 444
AcyI GRCGYC 3 cut(s) 179, 243, 255
AdeI CACNNNGTG 1 cut(s) 391
AfaI GTAC 4 cut(s) 422, 665, 800, 837
AfiI CCNNNNNNNGG 2 cut(s) 13, 523
AflII CTTAAG 2 cut(s) 547, 890
AgsI TTSAA 5 cut(s) 58, 602, 706, 805, 830
AjnI CCWGG 1 cut(s) 517
AluBI AGCT 9 cut(s) 35, 171, 231, 436, 512, 742, 842, 926, 998
AluI AGCT 9 cut(s) 35, 171, 231, 436, 512, 742, 842, 926, 998
Alw21I GWGCWC 4 cut(s) 37, 173, 233, 723
Alw26I GTCTC 3 cut(s) 341, 463, 558
AlwI GGATC 2 cut(s) 17, 593
AlwNI CAGNNNCTG 1 cut(s) 845
AoxI GGCC 5 cut(s) 27, 46, 287, 320, 777
ApeKI GCWGC 5 cut(s) 86, 509, 538, 541, 871
ApoI RAATTY 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 2 cut(s) 187, 920
AsuII TTCGAA 1 cut(s) 442
AsuNHI GCTAGC 1 cut(s) 742
AvaII GGWCC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 322
BanI GGYRCC 1 cut(s) 480
BanII GRGCYC 3 cut(s) 37, 173, 233
BauI CACGAG 1 cut(s) 647
BbsI GAAGAC 2 cut(s) 404, 691
Bbv12I GWGCWC 4 cut(s) 37, 173, 233, 723
BbvI GCAGC 5 cut(s) 73, 521, 525, 528, 858
BccI CCATC 1 cut(s) 190
BceAI ACGGC 3 cut(s) 242, 325, 468
BcgI CGANNNNNNTGC 2 cut(s) 348, 382
BciT130I CCWGG 1 cut(s) 519
BcnI CCSGG 1 cut(s) 752
BcoDI GTCTC 3 cut(s) 341, 463, 558
BfaI CTAG 2 cut(s) 743, 767
BfmI CTRYAG 1 cut(s) 849
BfrI CTTAAG 2 cut(s) 547, 890
BisI GCNGC 8 cut(s) 15, 87, 197, 200, 510, 539, 542, 872
BlsI GCNGC 8 cut(s) 16, 88, 198, 201, 511, 540, 543, 873
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 2 cut(s) 519, 752
Bme18I GGWCC 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 482
BmrFI CCNGG 2 cut(s) 519, 752
BmsI GCATC 4 cut(s) 483, 612, 871, 962
BmtI GCTAGC 1 cut(s) 746
BpiI GAAGAC 2 cut(s) 404, 691
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 2 cut(s) 139, 886
Bpu14I TTCGAA 1 cut(s) 442
BpuMI CCSGG 1 cut(s) 752
Bsa29I ATCGAT 2 cut(s) 368, 372
BsaHI GRCGYC 3 cut(s) 179, 243, 255
BsaI GGTCTC 1 cut(s) 558
BsaJI CCNNGG 4 cut(s) 43, 323, 341, 517
BsaWI WCCGGW 1 cut(s) 6
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 523
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 681, 869
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 1 cut(s) 519
BseCI ATCGAT 2 cut(s) 368, 372
BseDI CCNNGG 4 cut(s) 43, 323, 341, 517
BseGI GGATG 5 cut(s) 168, 182, 627, 901, 943
BseLI CCNNNNNNNGG 2 cut(s) 13, 523
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 2 cut(s) 306, 368
BseNI ACTGG 2 cut(s) 681, 869
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 5 cut(s) 73, 521, 525, 528, 858
BseYI CCCAGC 1 cut(s) 994
BsgI GTGCAG 3 cut(s) 527, 710, 936
Bsh1236I CGCG 1 cut(s) 14
Bsh1285I CGRYCG 2 cut(s) 84, 372
BshFI GGCC 5 cut(s) 29, 48, 289, 322, 779
BshNI GGYRCC 1 cut(s) 480
BshVI ATCGAT 2 cut(s) 368, 372
BsiEI CGRYCG 2 cut(s) 84, 372
BsiHKAI GWGCWC 4 cut(s) 37, 173, 233, 723
BsiSI CCGG 6 cut(s) 7, 30, 49, 80, 206, 751
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 2 cut(s) 13, 523
BsmAI GTCTC 3 cut(s) 341, 463, 558
BsmBI CGTCTC 1 cut(s) 463
BsmFI GGGAC 1 cut(s) 500
BsmI GAATGC 1 cut(s) 540
BsnI GGCC 5 cut(s) 29, 48, 289, 322, 779
Bso31I GGTCTC 1 cut(s) 558
Bsp119I TTCGAA 1 cut(s) 442
Bsp1286I GDGCHC 4 cut(s) 37, 173, 233, 723
Bsp143I GATC 5 cut(s) 9, 294, 369, 373, 585
Bsp19I CCATGG 3 cut(s) 43, 323, 341
BspACI CCGC 9 cut(s) 14, 61, 105, 121, 135, 197, 200, 209, 234
BspANI GGCC 5 cut(s) 29, 48, 289, 322, 779
BspCNI CTCAG 2 cut(s) 307, 369
BspDI ATCGAT 2 cut(s) 368, 372
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 1 cut(s) 482
BspOI GCTAGC 1 cut(s) 746
BspPI GGATC 2 cut(s) 17, 593
BspT104I TTCGAA 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 480
BspTI CTTAAG 2 cut(s) 547, 890
BspTNI GGTCTC 1 cut(s) 558
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 2 cut(s) 681, 869
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 4 cut(s) 43, 323, 341, 517
BssMI GATC 5 cut(s) 9, 294, 369, 373, 585
BssNI GRCGYC 3 cut(s) 179, 243, 255
BssSI CACGAG 1 cut(s) 647
BssT1I CCWWGG 3 cut(s) 43, 323, 341
Bst2BI CACGAG 1 cut(s) 647
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 5 cut(s) 430, 474, 505, 688, 907
Bst6I CTCTTC 1 cut(s) 765
BstACI GRCGYC 3 cut(s) 179, 243, 255
BstAFI CTTAAG 2 cut(s) 547, 890
BstAPI GCANNNNNTGC 1 cut(s) 391
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 3 cut(s) 383, 657, 744
BstDEI CTNAG 2 cut(s) 315, 377
BstDSI CCRYGG 3 cut(s) 43, 323, 341
BstF5I GGATG 5 cut(s) 168, 182, 627, 901, 943
BstFNI CGCG 1 cut(s) 14
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 5 cut(s) 12, 297, 372, 376, 588
BstMAI GTCTC 3 cut(s) 341, 463, 558
BstMBI GATC 5 cut(s) 9, 294, 369, 373, 585
BstMCI CGRYCG 2 cut(s) 84, 372
BstMWI GCNNNNNNNGC 3 cut(s) 242, 391, 923
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 2 cut(s) 517, 750
BstSFI CTRYAG 1 cut(s) 849
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 5 cut(s) 73, 521, 525, 528, 858
BstV2I GAAGAC 2 cut(s) 404, 691
BstXI CCANNNNNNTGG 1 cut(s) 575
Bsu15I ATCGAT 2 cut(s) 368, 372
BsuRI GGCC 5 cut(s) 29, 48, 289, 322, 779
BsuTUI ATCGAT 2 cut(s) 368, 372
BtgI CCRYGG 3 cut(s) 43, 323, 341
BtsCI GGATG 5 cut(s) 168, 182, 627, 901, 943
BtsIMutI CAGTG 2 cut(s) 684, 903
Cac8I GCNNGC 3 cut(s) 383, 657, 744
CaiI CAGNNNCTG 1 cut(s) 845
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 217
ClaI ATCGAT 2 cut(s) 368, 372
CseI GACGC 3 cut(s) 187, 251, 263
Csp6I GTAC 4 cut(s) 421, 664, 799, 836
CviAII CATG 6 cut(s) 44, 324, 337, 342, 389, 660
CviQI GTAC 4 cut(s) 421, 664, 799, 836
DdeI CTNAG 2 cut(s) 315, 377
DpnI GATC 5 cut(s) 11, 296, 371, 375, 587
DpnII GATC 5 cut(s) 9, 294, 369, 373, 585
DraIII CACNNNGTG 1 cut(s) 391
EaeI YGGCCR 3 cut(s) 27, 46, 320
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
EciI GGCGGA 2 cut(s) 223, 224
Ecl136II GAGCTC 3 cut(s) 35, 171, 231
Eco130I CCWWGG 3 cut(s) 43, 323, 341
Eco24I GRGCYC 3 cut(s) 37, 173, 233
Eco31I GGTCTC 1 cut(s) 558
Eco32I GATATC 3 cut(s) 457, 764, 1010
Eco47I GGWCC 1 cut(s) 217
Eco53kI GAGCTC 3 cut(s) 35, 171, 231
EcoICRI GAGCTC 3 cut(s) 35, 171, 231
EcoRI GAATTC 1 cut(s) 444
EcoRII CCWGG 1 cut(s) 517
EcoRV GATATC 3 cut(s) 457, 764, 1010
EcoT14I CCWWGG 3 cut(s) 43, 323, 341
EcoT38I GRGCYC 3 cut(s) 37, 173, 233
ErhI CCWWGG 3 cut(s) 43, 323, 341
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 6 cut(s) 47, 327, 340, 345, 392, 663
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FaqI GGGAC 1 cut(s) 500
FatI CATG 6 cut(s) 43, 323, 336, 341, 388, 659
Fnu4HI GCNGC 8 cut(s) 15, 87, 197, 200, 510, 539, 542, 872
FokI GGATG 5 cut(s) 169, 175, 634, 908, 950
FriOI GRGCYC 3 cut(s) 37, 173, 233
Fsp4HI GCNGC 8 cut(s) 15, 87, 197, 200, 510, 539, 542, 872
FspBI CTAG 2 cut(s) 743, 767
GlaI GCGC 1 cut(s) 41
GluI GCNGC 8 cut(s) 15, 87, 197, 200, 510, 539, 542, 872
GsaI CCCAGC 1 cut(s) 998
GsuI CTGGAG 2 cut(s) 139, 886
HaeIII GGCC 5 cut(s) 29, 48, 289, 322, 779
HapII CCGG 6 cut(s) 7, 30, 49, 80, 206, 751
HgaI GACGC 3 cut(s) 187, 251, 263
HhaI GCGC 1 cut(s) 42
Hin1I GRCGYC 3 cut(s) 179, 243, 255
Hin1II CATG 6 cut(s) 47, 327, 340, 345, 392, 663
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 2 cut(s) 696, 910
HpaII CCGG 6 cut(s) 7, 30, 49, 80, 206, 751
HphI GGTGA 2 cut(s) 187, 920
Hpy166II GTNNAC 1 cut(s) 943
Hpy188I TCNGA 8 cut(s) 117, 241, 316, 378, 450, 468, 560, 958
Hpy188III TCNNGA 3 cut(s) 407, 649, 767
Hpy8I GTNNAC 1 cut(s) 943
Hpy99I CGWCG 2 cut(s) 245, 263
HpyAV CCTTC 4 cut(s) 208, 217, 721, 799
HpyCH4III ACNGT 5 cut(s) 430, 474, 505, 688, 907
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 8 cut(s) 89, 488, 544, 625, 659, 691, 917, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 391, 923
HpyF3I CTNAG 2 cut(s) 315, 377
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 3 cut(s) 179, 243, 255
Hsp92II CATG 6 cut(s) 47, 327, 340, 345, 392, 663
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 5 cut(s) 9, 294, 369, 373, 585
LmnI GCTCC 4 cut(s) 32, 236, 847, 1003
Lsp1109I GCAGC 5 cut(s) 73, 521, 525, 528, 858
LweI GCATC 4 cut(s) 483, 612, 871, 962
MaeI CTAG 2 cut(s) 743, 767
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 175
MalI GATC 5 cut(s) 11, 296, 371, 375, 587
MboI GATC 5 cut(s) 9, 294, 369, 373, 585
MboII GAAGA 7 cut(s) 409, 431, 451, 614, 691, 694, 782
MfeI CAATTG 1 cut(s) 855
MhlI GDGCHC 4 cut(s) 37, 173, 233, 723
MlsI TGGCCA 1 cut(s) 322
MluCI AATT 5 cut(s) 328, 444, 825, 855, 980
MluNI TGGCCA 1 cut(s) 322
MlyI GAGTC 1 cut(s) 705
MmeI TCCRAC 6 cut(s) 264, 473, 508, 563, 739, 985
Mox20I TGGCCA 1 cut(s) 322
MroXI GAANNNNTTC 1 cut(s) 332
MscI TGGCCA 1 cut(s) 322
MseI TTAA 3 cut(s) 282, 548, 891
MslI CAYNNNNRTG 1 cut(s) 936
Msp20I TGGCCA 1 cut(s) 322
MspA1I CMGCKG 1 cut(s) 512
MspCI CTTAAG 2 cut(s) 547, 890
MspI CCGG 6 cut(s) 7, 30, 49, 80, 206, 751
MspR9I CCNGG 2 cut(s) 519, 752
MunI CAATTG 1 cut(s) 855
Mva1269I GAATGC 1 cut(s) 540
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 242, 391, 923
NciI CCSGG 1 cut(s) 752
NcoI CCATGG 3 cut(s) 43, 323, 341
NdeII GATC 5 cut(s) 9, 294, 369, 373, 585
NheI GCTAGC 1 cut(s) 742
NlaIII CATG 6 cut(s) 47, 327, 340, 345, 392, 663
NlaIV GGNNCC 1 cut(s) 482
NmuCI GTSAC 1 cut(s) 175
NspV TTCGAA 1 cut(s) 442
PctI GAATGC 1 cut(s) 540
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 1 cut(s) 910
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 8 cut(s) 16, 88, 198, 201, 511, 540, 543, 873
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 1 cut(s) 704
PpsI GAGTC 1 cut(s) 704
Psp124BI GAGCTC 3 cut(s) 37, 173, 233
Psp6I CCWGG 1 cut(s) 517
PspFI CCCAGC 1 cut(s) 994
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 1 cut(s) 482
PspPI GGNCC 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 845
PsyI GACNNNGTC 1 cut(s) 257
PvuI CGATCG 1 cut(s) 372
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 4 cut(s) 422, 665, 800, 837
RsaNI GTAC 4 cut(s) 421, 664, 799, 836
RseI CAYNNNNRTG 1 cut(s) 936
SacI GAGCTC 3 cut(s) 37, 173, 233
SaqAI TTAA 3 cut(s) 282, 548, 891
SatI GCNGC 8 cut(s) 15, 87, 197, 200, 510, 539, 542, 872
Sau3AI GATC 5 cut(s) 9, 294, 369, 373, 585
Sau96I GGNCC 1 cut(s) 217
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 1 cut(s) 705
ScrFI CCNGG 2 cut(s) 519, 752
SduI GDGCHC 4 cut(s) 37, 173, 233, 723
SfaNI GCATC 4 cut(s) 483, 612, 871, 962
SfcI CTRYAG 1 cut(s) 849
SfuI TTCGAA 1 cut(s) 442
SinI GGWCC 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 936
SmlI CTYRAG 2 cut(s) 547, 890
SmoI CTYRAG 2 cut(s) 547, 890
Sse9I AATT 5 cut(s) 328, 444, 825, 855, 980
SsiI CCGC 9 cut(s) 14, 61, 105, 121, 135, 197, 200, 209, 234
SspMI CTAG 2 cut(s) 743, 767
SstI GAGCTC 3 cut(s) 37, 173, 233
StyD4I CCNGG 2 cut(s) 517, 750
StyI CCWWGG 3 cut(s) 43, 323, 341
TaaI ACNGT 5 cut(s) 430, 474, 505, 688, 907
TaiI ACGT 1 cut(s) 404
TaqI TCGA 4 cut(s) 71, 368, 372, 442
TasI AATT 5 cut(s) 328, 444, 825, 855, 980
TatI WGTACW 1 cut(s) 798
TauI GCSGC 3 cut(s) 17, 199, 202
TfiI GAWTC 1 cut(s) 910
Tru1I TTAA 3 cut(s) 282, 548, 891
Tru9I TTAA 3 cut(s) 282, 548, 891
TscAI CASTG 2 cut(s) 691, 910
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 5 cut(s) 86, 509, 538, 541, 871
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 4 cut(s) 17, 325, 936, 953
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 2 cut(s) 691, 910
Tth111I GACNNNGTC 1 cut(s) 257
Vha464I CTTAAG 2 cut(s) 547, 890
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 1 cut(s) 444
XbaI TCTAGA 1 cut(s) 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 2 cut(s) 743, 767
ZrmI AGTACT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.