MD13G1231500.v1.1

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004710
Physical Location & Seq
Reverse (-)
22989592 .. 22991566
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1231500.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGAACCGGGTCGTGTTGAGGTCGGTGGTCGGAGGTCTCGTCAATGGCCGGAGCTGCAATCGCTACATTTCTACGGCGGTCGCGGTGGATAGGCCGTTGGAGAGCTCGGAGTTTCTGAGTCGCCAGAACGGCGGGGTTTTGGGAGCGGTTCAGTGGAGGAGTAGGATGCTGATGAGCACGTCGTCGTCGTCAGGGCAAGCGACGTCGTTGGCGGAGAAGGAGCAGAAGGAGGAGAATGAGAGCACAAACGGCAACAATAGCGTTGACGTTTCGAGCTACTGGGGGATTCAGGGGCCCAAGATTAGGAGAGAGGACGGGACTGAGTGGCCGTGGAATTGCTTCAAGCCGTGGGAGACTTATGAGGCGGACTTATCGATTGATCTGAGCAAGCATCATGTGCCGAAGACATTTCTGGACAGAGTTGCGTTTAGGACGGTCAAGTCTCTTCGAGTTCTATCGGATCTTTACTTCCAGCAACGGCACGGATGCCATGCAATGATGCTGGAAACCGTGGCAGCAGTCCCCGGTATGGTAGGAGGAATGTTGCTGCATTTGAAGTCTCTCCGCAAGTTTGAGCACAGCGGCGGTTGGATCAAAGCTTTACTTGAAGAGGCAGAGAACGAGAGGATGCACCTGATGACGGTGGTGGAACTTGTGAAGCCCGCCTGGCACGAGAGGCTTCTGGTTCTTGCCGTGCAGGGTGTCTTCTTCAATGCCTTCTTCGGGCTTTATCTGGCTTCCCCCAAACTGGCTCATAGGGTTGTCGGTTATCTGGAGGAGGAGGCTGTGTTCTCCTATACAGAGTACTTGAAGGATATCGAAAATGGCAAAATTGAAAATGTAAAGGCTCCTGAAATTGCAATCGATTACTGGAGGCTGCCTAAAGGCGCGACACTCCACGATGTTATAACTGTGATTCGCGCTGATGAAGCTCACCATCGCGATGTCAACCATTTTGCATCTGATATTCATTTTCAGGGAAAACAATTGAGAGACGCACCAGCTCCCCTTGGATATCACTAA

Protein Analysis

341

Amino Acids

38.43

Weight (kDa)

6.74

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 129 - 321 3.4e-81 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 908
AasI GACNNNNNNGTC 1 cut(s) 439
AatII GACGTC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 146
AccII CGCG 4 cut(s) 83, 890, 921, 942
AclWI GGATC 2 cut(s) 468, 599
AcoI YGGCCR 2 cut(s) 46, 326
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 5 cut(s) 303, 529, 640, 723, 748
AgsI TTSAA 6 cut(s) 343, 556, 608, 712, 811, 836
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 1 cut(s) 665
AloI GAACNNNNNNTCC 2 cut(s) 773, 805
AluBI AGCT 6 cut(s) 54, 105, 276, 599, 932, 1004
AluI AGCT 6 cut(s) 54, 105, 276, 599, 932, 1004
Alw21I GWGCWC 4 cut(s) 107, 179, 245, 579
Alw26I GTCTC 5 cut(s) 41, 347, 447, 564, 987
AlwI GGATC 2 cut(s) 468, 599
AoxI GGCC 4 cut(s) 46, 92, 293, 326
ApaI GGGCCC 1 cut(s) 297
ApeKI GCWGC 4 cut(s) 54, 515, 547, 877
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 2 cut(s) 890, 923
AspS9I GGNCC 2 cut(s) 293, 294
AsuC2I CCSGG 2 cut(s) 8, 525
AsuHPI GGTGA 1 cut(s) 926
BaeGI GKGCMC 1 cut(s) 297
BanII GRGCYC 2 cut(s) 107, 297
BauI CACGAG 1 cut(s) 671
BbsI GAAGAC 2 cut(s) 410, 697
Bbv12I GWGCWC 4 cut(s) 107, 179, 245, 579
BbvI GCAGC 4 cut(s) 41, 527, 534, 864
BccI CCATC 1 cut(s) 945
BceAI ACGGC 8 cut(s) 79, 90, 145, 265, 313, 331, 494, 677
BciT130I CCWGG 1 cut(s) 667
BcnI CCSGG 2 cut(s) 8, 525
BcoDI GTCTC 5 cut(s) 41, 347, 447, 564, 987
BglI GCCNNNNNGGC 2 cut(s) 129, 667
BisI GCNGC 5 cut(s) 55, 516, 548, 583, 878
BlsI GCNGC 5 cut(s) 56, 517, 549, 584, 879
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 3 cut(s) 8, 525, 667
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 293, 294
BmiI GGNNCC 3 cut(s) 294, 295, 849
BmrFI CCNGG 3 cut(s) 8, 525, 667
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 6 cut(s) 156, 400, 476, 489, 618, 968
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 2 cut(s) 410, 697
BpmI CTGGAG 2 cut(s) 794, 892
BpuMI CCSGG 2 cut(s) 8, 525
Bsa29I ATCGAT 2 cut(s) 374, 864
BsaHI GRCGYC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 5 cut(s) 329, 347, 510, 523, 1009
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 773, 803
Bsc4I CCNNNNNNNGG 5 cut(s) 303, 529, 640, 723, 748
Bse1I ACTGG 3 cut(s) 284, 753, 875
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 667
BseCI ATCGAT 2 cut(s) 374, 864
BseDI CCNNGG 5 cut(s) 329, 347, 510, 523, 1009
BseGI GGATG 3 cut(s) 171, 491, 633
BseLI CCNNNNNNNGG 5 cut(s) 303, 529, 640, 723, 748
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 107, 312, 374
BseNI ACTGG 3 cut(s) 284, 753, 875
BseRI GAGGAG 4 cut(s) 172, 245, 791, 794
BseSI GKGCMC 1 cut(s) 297
BseXI GCAGC 4 cut(s) 41, 527, 534, 864
BsgI GTGCAG 1 cut(s) 716
Bsh1236I CGCG 4 cut(s) 83, 890, 921, 942
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 4 cut(s) 48, 94, 295, 328
BshVI ATCGAT 2 cut(s) 374, 864
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 4 cut(s) 107, 179, 245, 579
BsiSI CCGG 3 cut(s) 7, 49, 525
BslFI GGGAC 2 cut(s) 331, 506
BslI CCNNNNNNNGG 5 cut(s) 303, 529, 640, 723, 748
BsmAI GTCTC 5 cut(s) 41, 347, 447, 564, 987
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 2 cut(s) 331, 506
BsnI GGCC 4 cut(s) 48, 94, 295, 328
Bso31I GGTCTC 1 cut(s) 41
Bsp120I GGGCCC 1 cut(s) 293
Bsp1286I GDGCHC 5 cut(s) 107, 179, 245, 297, 579
Bsp143I GATC 3 cut(s) 379, 460, 591
Bsp68I TCGCGA 1 cut(s) 942
BspANI GGCC 4 cut(s) 48, 94, 295, 328
BspCNI CTCAG 3 cut(s) 108, 313, 375
BspDI ATCGAT 2 cut(s) 374, 864
BspFNI CGCG 4 cut(s) 83, 890, 921, 942
BspLI GGNNCC 3 cut(s) 294, 295, 849
BspPI GGATC 2 cut(s) 468, 599
BspTNI GGTCTC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 3 cut(s) 284, 753, 875
BssECI CCNNGG 5 cut(s) 329, 347, 510, 523, 1009
BssMI GATC 3 cut(s) 379, 460, 591
BssNI GRCGYC 1 cut(s) 203
BssSI CACGAG 1 cut(s) 671
BssT1I CCWWGG 1 cut(s) 1009
Bst2BI CACGAG 1 cut(s) 671
Bst2UI CCWGG 1 cut(s) 667
Bst4CI ACNGT 4 cut(s) 436, 511, 643, 913
Bst6I CTCTTC 2 cut(s) 450, 603
BstACI GRCGYC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 397
BstC8I GCNNGC 3 cut(s) 198, 389, 663
BstDEI CTNAG 3 cut(s) 116, 321, 383
BstDSI CCRYGG 3 cut(s) 329, 347, 510
BstF5I GGATG 3 cut(s) 171, 491, 633
BstFNI CGCG 4 cut(s) 83, 890, 921, 942
BstHHI GCGC 2 cut(s) 890, 923
BstKTI GATC 3 cut(s) 382, 463, 594
BstMAI GTCTC 5 cut(s) 41, 347, 447, 564, 987
BstMBI GATC 3 cut(s) 379, 460, 591
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 9 cut(s) 54, 60, 129, 249, 258, 397, 667, 676, 929
BstNI CCWGG 1 cut(s) 667
BstSCI CCNGG 3 cut(s) 6, 523, 665
BstSLI GKGCMC 1 cut(s) 297
BstUI CGCG 4 cut(s) 83, 890, 921, 942
BstV1I GCAGC 4 cut(s) 41, 527, 534, 864
BstV2I GAAGAC 2 cut(s) 410, 697
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
Bsu15I ATCGAT 2 cut(s) 374, 864
BsuRI GGCC 4 cut(s) 48, 94, 295, 328
BsuTUI ATCGAT 2 cut(s) 374, 864
BtgI CCRYGG 3 cut(s) 329, 347, 510
BtgZI GCGATG 2 cut(s) 923, 957
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 3 cut(s) 171, 491, 633
BtsIMutI CAGTG 1 cut(s) 158
BtuMI TCGCGA 1 cut(s) 942
Cac8I GCNNGC 3 cut(s) 198, 389, 663
CfoI GCGC 2 cut(s) 890, 923
Cfr13I GGNCC 2 cut(s) 293, 294
ClaI ATCGAT 2 cut(s) 374, 864
CseI GACGC 1 cut(s) 1004
Csp6I GTAC 1 cut(s) 805
CviAII CATG 2 cut(s) 395, 491
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 3 cut(s) 116, 321, 383
DpnI GATC 3 cut(s) 381, 462, 593
DpnII GATC 3 cut(s) 379, 460, 591
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 2 cut(s) 46, 326
Eam1104I CTCTTC 2 cut(s) 450, 603
EarI CTCTTC 2 cut(s) 450, 603
EciI GGCGGA 2 cut(s) 227, 380
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 1009
Eco24I GRGCYC 2 cut(s) 107, 297
Eco31I GGTCTC 1 cut(s) 41
Eco32I GATATC 2 cut(s) 817, 1016
Eco53kI GAGCTC 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 665
EcoRV GATATC 2 cut(s) 817, 1016
EcoT14I CCWWGG 1 cut(s) 1009
EcoT38I GRGCYC 2 cut(s) 107, 297
ErhI CCWWGG 1 cut(s) 1009
Esp3I CGTCTC 1 cut(s) 987
FaeI CATG 2 cut(s) 398, 494
FaiI YATR 7 cut(s) 360, 396, 492, 530, 756, 798, 908
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 2 cut(s) 331, 506
FatI CATG 2 cut(s) 394, 490
FauI CCCGC 2 cut(s) 125, 670
Fnu4HI GCNGC 5 cut(s) 55, 516, 548, 583, 878
FokI GGATG 3 cut(s) 178, 498, 640
FriOI GRGCYC 2 cut(s) 107, 297
Fsp4HI GCNGC 5 cut(s) 55, 516, 548, 583, 878
GlaI GCGC 2 cut(s) 889, 922
GluI GCNGC 5 cut(s) 55, 516, 548, 583, 878
GsuI CTGGAG 2 cut(s) 794, 892
HaeIII GGCC 4 cut(s) 48, 94, 295, 328
HapII CCGG 3 cut(s) 7, 49, 525
HgaI GACGC 1 cut(s) 1004
HhaI GCGC 2 cut(s) 890, 923
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 2 cut(s) 398, 494
Hin6I GCGC 2 cut(s) 888, 921
HinP1I GCGC 2 cut(s) 888, 921
HincII GTYRAC 2 cut(s) 265, 949
HindII GTYRAC 2 cut(s) 265, 949
HindIII AAGCTT 1 cut(s) 597
HinfI GANTC 3 cut(s) 118, 286, 916
HpaII CCGG 3 cut(s) 7, 49, 525
HphI GGTGA 1 cut(s) 926
Hpy166II GTNNAC 2 cut(s) 265, 949
Hpy188I TCNGA 6 cut(s) 32, 109, 117, 384, 460, 964
Hpy188III TCNNGA 4 cut(s) 413, 773, 851, 941
Hpy8I GTNNAC 2 cut(s) 265, 949
Hpy99I CGWCG 5 cut(s) 184, 187, 190, 205, 208
HpyAV CCTTC 4 cut(s) 211, 220, 727, 805
HpyCH4III ACNGT 4 cut(s) 436, 511, 643, 913
HpyCH4IV ACGT 3 cut(s) 179, 203, 267
HpyCH4V TGCA 7 cut(s) 57, 494, 550, 631, 697, 860, 959
HpyF10VI GCNNNNNNNGC 9 cut(s) 54, 60, 129, 249, 258, 397, 667, 676, 929
HpyF3I CTNAG 3 cut(s) 116, 321, 383
HpySE526I ACGT 3 cut(s) 179, 203, 267
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 2 cut(s) 398, 494
HspAI GCGC 2 cut(s) 888, 921
Kzo9I GATC 3 cut(s) 379, 460, 591
LmnI GCTCC 5 cut(s) 51, 143, 220, 853, 1009
Lsp1109I GCAGC 4 cut(s) 41, 527, 534, 864
LweI GCATC 6 cut(s) 156, 400, 476, 489, 618, 968
MaeII ACGT 3 cut(s) 179, 203, 267
MalI GATC 3 cut(s) 381, 462, 593
MbiI CCGCTC 1 cut(s) 146
MboI GATC 3 cut(s) 379, 460, 591
MboII GAAGA 6 cut(s) 415, 437, 620, 697, 700, 712
MfeI CAATTG 1 cut(s) 986
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 5 cut(s) 107, 179, 245, 297, 579
MluCI AATT 4 cut(s) 334, 831, 855, 986
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 3 cut(s) 10, 78, 569
MroXI GAANNNNTTC 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 942
MspA1I CMGCKG 1 cut(s) 582
MspI CCGG 3 cut(s) 7, 49, 525
MspR9I CCNGG 3 cut(s) 8, 525, 667
MunI CAATTG 1 cut(s) 986
MvaI CCWGG 1 cut(s) 667
MvnI CGCG 4 cut(s) 83, 890, 921, 942
MwoI GCNNNNNNNGC 9 cut(s) 54, 60, 129, 249, 258, 397, 667, 676, 929
NciI CCSGG 2 cut(s) 8, 525
NdeII GATC 3 cut(s) 379, 460, 591
NlaIII CATG 2 cut(s) 398, 494
NlaIV GGNNCC 3 cut(s) 294, 295, 849
NruI TCGCGA 1 cut(s) 942
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 185
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 2 cut(s) 286, 916
PkrI GCNGC 5 cut(s) 56, 517, 549, 584, 879
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
PsiI TTATAA 1 cut(s) 908
Psp124BI GAGCTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 665
PspGI CCWGG 1 cut(s) 665
PspN4I GGNNCC 3 cut(s) 294, 295, 849
PspOMI GGGCCC 1 cut(s) 293
PspPI GGNCC 2 cut(s) 293, 294
PsuI RGATCY 1 cut(s) 460
RruI TCGCGA 1 cut(s) 942
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
RseI CAYNNNNRTG 1 cut(s) 942
SacI GAGCTC 1 cut(s) 107
SatI GCNGC 5 cut(s) 55, 516, 548, 583, 878
Sau3AI GATC 3 cut(s) 379, 460, 591
Sau96I GGNCC 2 cut(s) 293, 294
ScaI AGTACT 1 cut(s) 806
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 3 cut(s) 8, 525, 667
SduI GDGCHC 5 cut(s) 107, 179, 245, 297, 579
SfaNI GCATC 6 cut(s) 156, 400, 476, 489, 618, 968
SmiMI CAYNNNNRTG 1 cut(s) 942
Sse9I AATT 4 cut(s) 334, 831, 855, 986
SstI GAGCTC 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 6, 523, 665
StyI CCWWGG 1 cut(s) 1009
TaaI ACNGT 4 cut(s) 436, 511, 643, 913
TaiI ACGT 3 cut(s) 182, 206, 270
TaqI TCGA 5 cut(s) 272, 374, 448, 819, 864
TasI AATT 4 cut(s) 334, 831, 855, 986
TatI WGTACW 1 cut(s) 804
TauI GCSGC 1 cut(s) 585
TfiI GAWTC 2 cut(s) 286, 916
TscAI CASTG 1 cut(s) 158
TseI GCWGC 4 cut(s) 54, 515, 547, 877
TspDTI ATGAA 3 cut(s) 17, 942, 959
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 338
ZraI GACGTC 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.