FvH4_4g10900

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006360
Physical Location & Seq
Forward (+)
14586721 .. 14591294
4574 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10900.t3

Sequence Viewer

Length: 1308 bp
ATGGGTTTGGAGGCAGTGGGAGATCTAGCGCTACACATAATCCTGCGCAAGTTAGGAGCTGAAGAGTCGGCGAGAGCAGCCTGCGTGAGCAAGCAACTCAGAGCTTCCGCCTCTGAAGACCCTCTTTGGTCCCATTTCTGTGCCCAGGACCTCCACCTCCACCAACCCTTGGATCCTCATGGCAACCTTGCCCCGTCTTTCAAGGCCAGTTATGGTTTGTGGCGGGAATCTTTCAGCATGTACCCATGGTCCCTTGTAAAGCGAGCCAAGAGGTGTTGGGCCAAAATCACAAACTGGCTCTCTCTTCATTTTCCAGAGGCTCACTCTTCCCTCAACAAGGGTGCATCAGAGGCTGACATTCAACACTTGGAGACCCAATTGAAAGTCAAACTTCCTCTCCCTACAAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTACACGATTCTCTACTGGGCCTTATTGGTGGTTACTCTTTTTATAACCACCTCGTCAATGTCTCCTTGTTGCCTTTACATCAAATAGTCTTAGAAACTAAGCATGTCATGCCAAAGCTAGGCTTTTCAGCCACACCAGATTATATTGTTGTGGCTTCTTCTTCTTCTACTTATAGCCAAAAGTTGTTTTTCCTCAACTGTACAACTGGCCAGCTCTATGTCGGTACCGCCAACCTTCTGCCTGATGCTGAAATGCTTCCCTGTGTACCCAATGCGTTACTTAATTCAGTTCATGATCACAATGGTGATCAACAACAAGATGGCATGCTTCTTTGGTTGGAAGAACACTACCGCCGTCTAGAAAATGGAATCATTAGACTCCGTGAACAAGAAGCTATGAGAAGCATCAGTCAGTTTCCAGAGGAATCCCCTCTCTGTTCCACTGCCGTAACAAATGGGGTACAGGTTCGTTGTTCTTCGGTGTTTGTCCCGGAGTTTTCTAACCTTCAAGATAACACCCAGAGGTACTATTTTTCCTATTCGATCCGCATGTCGCTTCTGCCTGAAGGATGTGGCATCAATGGAATGACATTTAACTCTTGCCAACTGCTTTCAAGGCACTGGGTCATTCGTGCTTATGATGTTGTGGTAGCTAATGTTAATGGAGAGGCTGTTATAGGAAAGTATCCTCTCTTGTGTCCAGGTGAGCAAGAATTTGTATATGAGAGTTGCACGCCTCTGCCATCTTCCTCGGGTTCTATAGAAGGTTCTTTCATGTTTGTCCCGGGCAGGTTGGCGGACCCAAAAGGCAGCAGATTTAGAGTTCCGGCTGCACGCTTTCCCCTCCAGCTGCCAGACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

436

Amino Acids

48.75

Weight (kDa)

6.38

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 342 - 434 2e-22 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
Acc16I TGCGCA 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 1218
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 1 cut(s) 169
AciI CCGC 6 cut(s) 108, 223, 666, 790, 985, 1235
AclWI GGATC 3 cut(s) 167, 180, 976
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 1 cut(s) 1151
AcuI CTGAAG 3 cut(s) 81, 135, 1023
AfaI GTAC 6 cut(s) 242, 640, 664, 705, 900, 965
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 3 cut(s) 169, 337, 557
AgsI TTSAA 5 cut(s) 202, 362, 382, 947, 1053
AjnI CCWGG 2 cut(s) 144, 1138
AleI CACNNNNGTG 1 cut(s) 741
AluBI AGCT 7 cut(s) 59, 104, 556, 652, 833, 1091, 1288
AluI AGCT 7 cut(s) 59, 104, 556, 652, 833, 1091, 1288
Alw26I GTCTC 2 cut(s) 365, 505
AlwI GGATC 3 cut(s) 167, 180, 976
AlwNI CAGNNNCTG 1 cut(s) 353
Ama87I CYCGRG 2 cut(s) 1189, 1222
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 4 cut(s) 204, 279, 457, 646
ApeKI GCWGC 4 cut(s) 77, 1248, 1268, 1288
ApoI RAATTY 1 cut(s) 1151
Asp700I GAANNNNTTC 1 cut(s) 693
Asp718I GGTACC 1 cut(s) 662
AspLEI GCGC 2 cut(s) 31, 48
AspS9I GGNCC 6 cut(s) 129, 148, 249, 279, 457, 1237
AsuC2I CCSGG 3 cut(s) 929, 1223, 1224
AsuHPI GGTGA 2 cut(s) 755, 1154
AvaI CYCGRG 2 cut(s) 1189, 1222
AvaII GGWCC 4 cut(s) 129, 148, 249, 1237
BaeGI GKGCMC 1 cut(s) 145
BalI TGGCCA 1 cut(s) 648
BamHI GGATCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 662
BbsI GAAGAC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 89, 1255, 1260, 1275
BccI CCATC 2 cut(s) 752, 1189
BceAI ACGGC 2 cut(s) 777, 869
BciT130I CCWGG 2 cut(s) 146, 1140
BciVI GTATCC 1 cut(s) 1134
BclI TGATCA 2 cut(s) 733, 745
BcnI CCSGG 3 cut(s) 929, 1223, 1224
BcoDI GTCTC 2 cut(s) 365, 505
BfaI CTAG 3 cut(s) 26, 557, 797
BfmI CTRYAG 1 cut(s) 1197
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 1218
BfuI GTATCC 1 cut(s) 1134
BglII AGATCT 1 cut(s) 22
BisI GCNGC 4 cut(s) 78, 1249, 1269, 1289
BlsI GCNGC 4 cut(s) 79, 1250, 1270, 1290
Bme1390I CCNGG 5 cut(s) 146, 929, 1140, 1223, 1224
Bme18I GGWCC 4 cut(s) 129, 148, 249, 1237
BmeT110I CYCGRG 2 cut(s) 1189, 1222
BmgT120I GGNCC 6 cut(s) 129, 148, 249, 279, 457, 1237
BmiI GGNNCC 5 cut(s) 131, 174, 251, 664, 1239
BmrFI CCNGG 5 cut(s) 146, 929, 1140, 1223, 1224
BmrI ACTGGG 2 cut(s) 464, 1069
BmsI GCATC 4 cut(s) 353, 673, 852, 1023
BmuI ACTGGG 2 cut(s) 464, 1069
BpiI GAAGAC 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 1 cut(s) 1268
BpuMI CCSGG 3 cut(s) 929, 1223, 1224
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 5 cut(s) 144, 168, 245, 1188, 1222
BsaXI ACNNNNNCTCC 4 cut(s) 381, 411, 1095, 1125
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 337, 557
Bse1I ACTGG 5 cut(s) 207, 299, 459, 649, 1064
BseBI CCWGG 2 cut(s) 146, 1140
BseDI CCNNGG 5 cut(s) 144, 168, 245, 1188, 1222
BseGI GGATG 1 cut(s) 1013
BseLI CCNNNNNNNGG 3 cut(s) 169, 337, 557
BseMII CTCAG 1 cut(s) 112
BseNI ACTGG 5 cut(s) 207, 299, 459, 649, 1064
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 4 cut(s) 89, 1255, 1260, 1275
BsgI GTGCAG 1 cut(s) 1254
BshFI GGCC 4 cut(s) 206, 281, 459, 648
BshNI GGYRCC 1 cut(s) 662
BsiHKCI CYCGRG 2 cut(s) 1189, 1222
BsiSI CCGG 3 cut(s) 929, 1223, 1265
BslFI GGGAC 4 cut(s) 115, 235, 911, 1205
BslI CCNNNNNNNGG 3 cut(s) 169, 337, 557
BsmAI GTCTC 2 cut(s) 365, 505
BsmFI GGGAC 4 cut(s) 115, 235, 911, 1205
BsnI GGCC 4 cut(s) 206, 281, 459, 648
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 2 cut(s) 1189, 1222
Bsp1286I GDGCHC 1 cut(s) 145
Bsp1407I TGTACA 1 cut(s) 638
Bsp143I GATC 5 cut(s) 22, 172, 733, 745, 981
Bsp19I CCATGG 1 cut(s) 245
BspACI CCGC 6 cut(s) 108, 223, 666, 790, 985, 1235
BspANI GGCC 4 cut(s) 206, 281, 459, 648
BspCNI CTCAG 1 cut(s) 111
BspHI TCATGA 2 cut(s) 424, 730
BspLI GGNNCC 5 cut(s) 131, 174, 251, 664, 1239
BspMI ACCTGC 1 cut(s) 1218
BspPI GGATC 3 cut(s) 167, 180, 976
BspT107I GGYRCC 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 365
BsrGI TGTACA 1 cut(s) 638
BsrI ACTGG 5 cut(s) 207, 299, 459, 649, 1064
BssECI CCNNGG 5 cut(s) 144, 168, 245, 1188, 1222
BssMI GATC 5 cut(s) 22, 172, 733, 745, 981
BssT1I CCWWGG 2 cut(s) 168, 245
Bst2UI CCWGG 2 cut(s) 146, 1140
Bst4CI ACNGT 2 cut(s) 431, 638
Bst6I CTCTTC 3 cut(s) 57, 309, 331
BstAPI GCANNNNNTGC 1 cut(s) 547
BstAUI TGTACA 1 cut(s) 638
BstC8I GCNNGC 7 cut(s) 82, 92, 264, 650, 764, 1172, 1273
BstDEI CTNAG 3 cut(s) 98, 529, 537
BstDSI CCRYGG 1 cut(s) 245
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 1013
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 48
BstKTI GATC 5 cut(s) 25, 175, 736, 748, 984
BstMAI GTCTC 2 cut(s) 365, 505
BstMBI GATC 5 cut(s) 22, 172, 733, 745, 981
BstMWI GCNNNNNNNGC 4 cut(s) 77, 350, 547, 1054
BstNI CCWGG 2 cut(s) 146, 1140
BstNSI RCATGY 4 cut(s) 241, 545, 766, 991
BstSCI CCNGG 5 cut(s) 144, 927, 1138, 1221, 1222
BstSFI CTRYAG 1 cut(s) 1197
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 4 cut(s) 89, 1255, 1260, 1275
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 22, 172
BstYI RGATCY 2 cut(s) 22, 172
BsuI GTATCC 1 cut(s) 1134
BsuRI GGCC 4 cut(s) 206, 281, 459, 648
BtgI CCRYGG 1 cut(s) 245
BtsCI GGATG 1 cut(s) 1013
BtsI GCAGTG 2 cut(s) 21, 879
BtsIMutI CAGTG 3 cut(s) 21, 879, 1057
BveI ACCTGC 1 cut(s) 1218
Cac8I GCNNGC 7 cut(s) 82, 92, 264, 650, 764, 1172, 1273
CaiI CAGNNNCTG 1 cut(s) 353
CciI TCATGA 2 cut(s) 424, 730
CfoI GCGC 2 cut(s) 31, 48
Cfr13I GGNCC 6 cut(s) 129, 148, 249, 279, 457, 1237
Cfr9I CCCGGG 1 cut(s) 1222
Csp6I GTAC 6 cut(s) 241, 639, 663, 704, 899, 964
CviQI GTAC 6 cut(s) 241, 639, 663, 704, 899, 964
DdeI CTNAG 3 cut(s) 98, 529, 537
DpnI GATC 5 cut(s) 24, 174, 735, 747, 983
DpnII GATC 5 cut(s) 22, 172, 733, 745, 981
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 3 cut(s) 57, 309, 331
EarI CTCTTC 3 cut(s) 57, 309, 331
EciI GGCGGA 2 cut(s) 97, 1250
Eco130I CCWWGG 2 cut(s) 168, 245
Eco31I GGTCTC 1 cut(s) 365
Eco47I GGWCC 4 cut(s) 129, 148, 249, 1237
Eco47III AGCGCT 1 cut(s) 30
Eco57I CTGAAG 3 cut(s) 81, 135, 1023
Eco88I CYCGRG 2 cut(s) 1189, 1222
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 144, 1138
EcoT14I CCWWGG 2 cut(s) 168, 245
ErhI CCWWGG 2 cut(s) 168, 245
FalI AAGNNNNNCTT 6 cut(s) 108, 140, 375, 407, 545, 577
FaqI GGGAC 4 cut(s) 115, 235, 911, 1205
FauI CCCGC 1 cut(s) 216
FbaI TGATCA 2 cut(s) 733, 745
Fnu4HI GCNGC 4 cut(s) 78, 1249, 1269, 1289
FokI GGATG 1 cut(s) 1020
Fsp4HI GCNGC 4 cut(s) 78, 1249, 1269, 1289
FspBI CTAG 3 cut(s) 26, 557, 797
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 30, 47
GluI GCNGC 4 cut(s) 78, 1249, 1269, 1289
GsuI CTGGAG 1 cut(s) 1268
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 4 cut(s) 206, 281, 459, 648
HapII CCGG 3 cut(s) 929, 1223, 1265
HhaI GCGC 2 cut(s) 31, 48
Hin6I GCGC 2 cut(s) 29, 46
HinP1I GCGC 2 cut(s) 29, 46
HinfI GANTC 7 cut(s) 65, 227, 409, 446, 807, 816, 863
HpaII CCGG 3 cut(s) 929, 1223, 1265
HphI GGTGA 2 cut(s) 755, 1154
Hpy166II GTNNAC 2 cut(s) 704, 824
Hpy188I TCNGA 4 cut(s) 101, 115, 349, 1307
Hpy188III TCNNGA 7 cut(s) 314, 425, 434, 731, 797, 857, 947
Hpy8I GTNNAC 2 cut(s) 704, 824
HpyAV CCTTC 4 cut(s) 683, 953, 998, 1196
HpyCH4III ACNGT 2 cut(s) 431, 638
HpyCH4V TGCA 3 cut(s) 344, 1170, 1271
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 350, 547, 1054
HpyF3I CTNAG 3 cut(s) 98, 529, 537
HspAI GCGC 2 cut(s) 29, 46
KpnI GGTACC 1 cut(s) 666
Ksp22I TGATCA 2 cut(s) 733, 745
Kzo9I GATC 5 cut(s) 22, 172, 733, 745, 981
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 4 cut(s) 89, 1255, 1260, 1275
LweI GCATC 4 cut(s) 353, 673, 852, 1023
MaeI CTAG 3 cut(s) 26, 557, 797
MaeIII GTNAC 3 cut(s) 470, 714, 886
MalI GATC 5 cut(s) 24, 174, 735, 747, 983
MboI GATC 5 cut(s) 22, 172, 733, 745, 981
MfeI CAATTG 1 cut(s) 377
MflI RGATCY 2 cut(s) 22, 172
MhlI GDGCHC 1 cut(s) 145
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 3 cut(s) 377, 721, 1151
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 2 cut(s) 74, 810
MmeI TCCRAC 1 cut(s) 756
Mox20I TGGCCA 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 693
MscI TGGCCA 1 cut(s) 648
MseI TTAA 3 cut(s) 720, 1032, 1098
MslI CAYNNNNRTG 2 cut(s) 138, 741
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 1288
MspI CCGG 3 cut(s) 929, 1223, 1265
MspR9I CCNGG 5 cut(s) 146, 929, 1140, 1223, 1224
MunI CAATTG 1 cut(s) 377
MvaI CCWGG 2 cut(s) 146, 1140
MwoI GCNNNNNNNGC 4 cut(s) 77, 350, 547, 1054
NciI CCSGG 3 cut(s) 929, 1223, 1224
NcoI CCATGG 1 cut(s) 245
NdeII GATC 5 cut(s) 22, 172, 733, 745, 981
NlaIV GGNNCC 5 cut(s) 131, 174, 251, 664, 1239
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 4 cut(s) 241, 545, 766, 991
OliI CACNNNNGTG 1 cut(s) 741
PaeI GCATGC 1 cut(s) 766
PagI TCATGA 2 cut(s) 424, 730
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 5 cut(s) 227, 409, 446, 807, 863
PflMI CCANNNNNTGG 1 cut(s) 169
PfoI TCCNGGA 1 cut(s) 927
PkrI GCNGC 4 cut(s) 79, 1250, 1270, 1290
PleI GAGTC 2 cut(s) 73, 810
PpsI GAGTC 2 cut(s) 73, 810
PpuMI RGGWCCY 1 cut(s) 148
PsiI TTATAA 1 cut(s) 483
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 2 cut(s) 144, 1138
PspGI CCWGG 2 cut(s) 144, 1138
PspN4I GGNNCC 5 cut(s) 131, 174, 251, 664, 1239
PspPI GGNCC 6 cut(s) 129, 148, 249, 279, 457, 1237
PspPPI RGGWCCY 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 2 cut(s) 22, 172
PvuII CAGCTG 1 cut(s) 1288
RsaI GTAC 6 cut(s) 242, 640, 664, 705, 900, 965
RsaNI GTAC 6 cut(s) 241, 639, 663, 704, 899, 964
RseI CAYNNNNRTG 2 cut(s) 138, 741
SaqAI TTAA 3 cut(s) 720, 1032, 1098
SatI GCNGC 4 cut(s) 78, 1249, 1269, 1289
Sau3AI GATC 5 cut(s) 22, 172, 733, 745, 981
Sau96I GGNCC 6 cut(s) 129, 148, 249, 279, 457, 1237
SchI GAGTC 2 cut(s) 74, 810
ScrFI CCNGG 5 cut(s) 146, 929, 1140, 1223, 1224
SduI GDGCHC 1 cut(s) 145
SfaNI GCATC 4 cut(s) 353, 673, 852, 1023
SfcI CTRYAG 1 cut(s) 1197
SinI GGWCC 4 cut(s) 129, 148, 249, 1237
SmaI CCCGGG 1 cut(s) 1224
SmiMI CAYNNNNRTG 2 cut(s) 138, 741
SphI GCATGC 1 cut(s) 766
Sse9I AATT 3 cut(s) 377, 721, 1151
SsiI CCGC 6 cut(s) 108, 223, 666, 790, 985, 1235
SspMI CTAG 3 cut(s) 26, 557, 797
StyD4I CCNGG 5 cut(s) 144, 927, 1138, 1221, 1222
StyI CCWWGG 2 cut(s) 168, 245
TaaI ACNGT 2 cut(s) 431, 638
TaqI TCGA 1 cut(s) 980
TasI AATT 3 cut(s) 377, 721, 1151
TatI WGTACW 1 cut(s) 638
TfiI GAWTC 5 cut(s) 227, 409, 446, 807, 863
Tru1I TTAA 3 cut(s) 720, 1032, 1098
Tru9I TTAA 3 cut(s) 720, 1032, 1098
TscAI CASTG 3 cut(s) 21, 886, 1064
TseI GCWGC 4 cut(s) 77, 1248, 1268, 1288
TspDTI ATGAA 4 cut(s) 296, 413, 719, 1201
TspGWI ACGGA 1 cut(s) 809
TspMI CCCGGG 1 cut(s) 1222
TspRI CASTG 3 cut(s) 21, 886, 1064
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 4 cut(s) 129, 148, 249, 1237
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 1 cut(s) 1151
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 4 cut(s) 241, 545, 766, 991
XmaI CCCGGG 1 cut(s) 1222
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 3 cut(s) 26, 557, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.