MD04G1044900.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006360
Physical Location & Seq
Reverse (-)
5227798 .. 5234434
6637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1044900.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGAATTTGGAGGCAGTGGGTGATCTTGCGCTGCACATAATCCTCAGCAAGTTAGGAGCGGAAGACTCAGCGAAAGCAGCATGCGTCAGCACCAAACTCAGAGCTTCCGCCTCCGATGAATCCCTTTGGTCCCTTTTCTGTGCTCGAGACCTTCACCTCTCGCAACCCCTCGACCCCCTTGGCAACCTCGCCCCTTCTTTCAAGGAAAGTTACCAACTATGGCGTGTATCTTTTAAAATGTATCCTTGGTCGCTTGTAAAGCGAGTCAAAAGATGCTGGGACAAAATTAGAAACTGGTTGACTATTAATTTTCCAGAGGCTGTTTCTACACTGAAAAAGGGTGCATCAGAAGCTAATATACAAGAGGTGGAGAAAATCTTGAAAGTGAAACTTCCTCTTCCAACAAGGATCCTGTATCGTTTTCATAATGGTCAAGCTTTTGAGGAAAAACATTTTCAAAATAGCCTGGTTGGCTGCCCATTGGGCCTCATAGGTGGTTATGCATTTTATGACCACTCGGTAACTGTGTATTTGATGCCTTTACGTCAGGTAGTCTTAGAAACAATGAAGATCAGACGTAAACTGGGTTTTACTGGTAGATCAGAATATGTTGTTGTGGCGGATTCTTGTACATATAGCGGGAAGTTATTTTTCCTGAACTGTACAAGTGGTCAACTCTATGTTGGTACCAGAAATCTTCCAACTGATGGGCAAATGCTTCCTTGCGTACCAGATGCGTTGATAAGTTCAATTCATGATTGCAATGTTGATCAACAGCAGGATGGCATGCTGCTTTGGTTAGAAGAGCATGGCCGTCGTTTAGAAAATGGCATTATTAAACTTCGTCAAGAAGAAAATTTCAGAACCATCAGTCAGTTTCCAGAAGAATCCCCTCTTTGTTCCACTGCTATAACAAATGGCGTAAAGGTTCGTGCTTCTGCTGTGTTTGTCCCAGAGCGTGCTGATCTTGAAAATACTTCTGAAAAGTATACATTTTCTTATTCGATCCGCATGTCCCTTCTTCCTGAAGGATGTGTGATTAATGGAATGCCATTCAGCTCTTGTCAGTTGAATTGGAGGCACTGGATCATTCGTTCTAATGATGTTGTGGTAGCTGATGTTGGAGGAGAAGCTGTTATTGGACAGTACCCACTCTTGCGTCCAGGTGAAGAGGAATTCGTTTATGAGAGTTACTCTTGTCTACCATCTTCTTCAGGTTCTATTGAAGGTTCTTTTACATTTGTCCCTGGCAGTTTGAGGGTGCCAAAGGGTGGCCCGTTTGAGGTTGCAGTGGCACGGTTTCCCCTCCAGCTGCCCAACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

444

Amino Acids

49.72

Weight (kDa)

7.06

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 324 - 415 2.5e-21 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 686
AccB1I GGYRCC 2 cut(s) 686, 1261
AccB7I CCANNNNNTGG 2 cut(s) 707, 1271
AccBSI CCGCTC 1 cut(s) 59
AccI GTMKAC 2 cut(s) 989, 1201
AciI CCGC 5 cut(s) 59, 108, 620, 639, 1009
AclWI GGATC 4 cut(s) 403, 416, 1000, 1094
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 3 cut(s) 4, 856, 1175
AcuI CTGAAG 2 cut(s) 1047, 1197
AfaI GTAC 5 cut(s) 631, 664, 688, 729, 1148
AfiI CCNNNNNNNGG 3 cut(s) 707, 1271, 1282
AgsI TTSAA 7 cut(s) 202, 382, 458, 750, 971, 1072, 1226
AjnI CCWGG 3 cut(s) 465, 1162, 1246
AjuI GAANNNNNNNTTGG 3 cut(s) 1122, 1154, 1310
AloI GAACNNNNNNTCC 2 cut(s) 1078, 1110
AluBI AGCT 7 cut(s) 104, 353, 437, 1059, 1115, 1133, 1312
AluI AGCT 7 cut(s) 104, 353, 437, 1059, 1115, 1133, 1312
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 4 cut(s) 403, 416, 1000, 1094
AlwNI CAGNNNCTG 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 3 cut(s) 484, 811, 1273
ApeKI GCWGC 5 cut(s) 31, 77, 474, 790, 1312
ApoI RAATTY 3 cut(s) 4, 856, 1175
AseI ATTAAT 2 cut(s) 306, 1041
Asp718I GGTACC 1 cut(s) 686
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 129, 484, 1274
AsuHPI GGTGA 3 cut(s) 32, 146, 1178
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 1 cut(s) 129
BamHI GGATCC 1 cut(s) 408
BanI GGYRCC 2 cut(s) 686, 1261
BarI GAAGNNNNNNTAC 2 cut(s) 342, 374
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 145
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 5 cut(s) 18, 89, 461, 777, 1299
BccI CCATC 4 cut(s) 701, 776, 875, 1213
BceAI ACGGC 1 cut(s) 798
BcgI CGANNNNNNTGC 2 cut(s) 797, 831
BciT130I CCWGG 3 cut(s) 467, 1164, 1248
BciVI GTATCC 1 cut(s) 252
BclI TGATCA 1 cut(s) 769
BcoDI GTCTC 1 cut(s) 141
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 2 cut(s) 471, 483
BisI GCNGC 5 cut(s) 32, 78, 475, 791, 1313
BlsI GCNGC 5 cut(s) 33, 79, 476, 792, 1314
Bme1390I CCNGG 3 cut(s) 467, 1164, 1248
Bme18I GGWCC 1 cut(s) 129
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 3 cut(s) 129, 484, 1274
BmiI GGNNCC 4 cut(s) 131, 410, 688, 1263
BmrFI CCNGG 3 cut(s) 467, 1164, 1248
BmrI ACTGGG 1 cut(s) 593
BmsI GCATC 4 cut(s) 263, 353, 525, 724
BmuI ACTGGG 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 1178, 1210
BpmI CTGGAG 1 cut(s) 1292
Bpu10I CCTNAGC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 178, 245, 1246
BsaXI ACNNNNNCTCC 2 cut(s) 1119, 1149
Bsc4I CCNNNNNNNGG 3 cut(s) 707, 1271, 1282
Bse1I ACTGG 4 cut(s) 299, 588, 598, 1088
Bse3DI GCAATG 1 cut(s) 769
BseBI CCWGG 3 cut(s) 467, 1164, 1248
BseDI CCNNGG 3 cut(s) 178, 245, 1246
BseGI GGATG 2 cut(s) 787, 1037
BseLI CCNNNNNNNGG 3 cut(s) 707, 1271, 1282
BseMI GCAATG 1 cut(s) 769
BseMII CTCAG 3 cut(s) 58, 81, 112
BseNI ACTGG 4 cut(s) 299, 588, 598, 1088
BseRI GAGGAG 1 cut(s) 1140
BseXI GCAGC 5 cut(s) 18, 89, 461, 777, 1299
BseYI CCCAGC 1 cut(s) 276
BsgI GTGCAG 1 cut(s) 17
BshFI GGCC 3 cut(s) 486, 813, 1275
BshNI GGYRCC 2 cut(s) 686, 1261
BsiHKAI GWGCWC 1 cut(s) 145
BsiHKCI CYCGRG 1 cut(s) 144
BslFI GGGAC 5 cut(s) 115, 293, 935, 1000, 1229
BslI CCNNNNNNNGG 3 cut(s) 707, 1271, 1282
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 5 cut(s) 115, 293, 935, 1000, 1229
BsmI GAATGC 1 cut(s) 1053
BsnI GGCC 3 cut(s) 486, 813, 1275
Bso31I GGTCTC 1 cut(s) 141
BsoBI CYCGRG 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 145
Bsp1407I TGTACA 2 cut(s) 629, 662
Bsp143I GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
BspACI CCGC 5 cut(s) 59, 108, 620, 639, 1009
BspANI GGCC 3 cut(s) 486, 813, 1275
BspCNI CTCAG 3 cut(s) 57, 80, 111
BspHI TCATGA 1 cut(s) 754
BspLI GGNNCC 4 cut(s) 131, 410, 688, 1263
BspPI GGATC 4 cut(s) 403, 416, 1000, 1094
BspQI GCTCTTC 1 cut(s) 798
BspT107I GGYRCC 2 cut(s) 686, 1261
BspTNI GGTCTC 1 cut(s) 141
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 769
BsrGI TGTACA 2 cut(s) 629, 662
BsrI ACTGG 4 cut(s) 299, 588, 598, 1088
BssECI CCNNGG 3 cut(s) 178, 245, 1246
BssMI GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
BssNAI GTATAC 1 cut(s) 990
BssT1I CCWWGG 2 cut(s) 178, 245
Bst1107I GTATAC 1 cut(s) 990
Bst2UI CCWGG 3 cut(s) 467, 1164, 1248
Bst4CI ACNGT 4 cut(s) 526, 662, 1146, 1299
Bst6I CTCTTC 3 cut(s) 402, 798, 1164
BstAUI TGTACA 2 cut(s) 629, 662
BstC8I GCNNGC 3 cut(s) 82, 788, 960
BstDEI CTNAG 4 cut(s) 44, 67, 98, 556
BstF5I GGATG 2 cut(s) 787, 1037
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 8 cut(s) 25, 411, 573, 602, 772, 967, 1008, 1089
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
BstMWI GCNNNNNNNGC 5 cut(s) 77, 259, 350, 471, 483
BstNI CCWGG 3 cut(s) 467, 1164, 1248
BstNSI RCATGY 3 cut(s) 84, 790, 1015
BstSCI CCNGG 3 cut(s) 465, 1162, 1246
BstV1I GCAGC 5 cut(s) 18, 89, 461, 777, 1299
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
BstZ17I GTATAC 1 cut(s) 990
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 486, 813, 1275
BtsCI GGATG 2 cut(s) 787, 1037
BtsI GCAGTG 3 cut(s) 21, 903, 1296
BtsIMutI CAGTG 5 cut(s) 21, 329, 903, 1081, 1296
Cac8I GCNNGC 3 cut(s) 82, 788, 960
CaiI CAGNNNCTG 1 cut(s) 320
CciI TCATGA 1 cut(s) 754
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 3 cut(s) 129, 484, 1274
CseI GACGC 2 cut(s) 73, 1148
Csp6I GTAC 5 cut(s) 630, 663, 687, 728, 1147
CviAII CATG 5 cut(s) 81, 755, 787, 809, 1012
CviQI GTAC 5 cut(s) 630, 663, 687, 728, 1147
DdeI CTNAG 4 cut(s) 44, 67, 98, 556
DpnI GATC 8 cut(s) 24, 410, 572, 601, 771, 966, 1007, 1088
DpnII GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
DraI TTTAAA 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 3 cut(s) 402, 798, 1164
EarI CTCTTC 3 cut(s) 402, 798, 1164
EciI GGCGGA 2 cut(s) 97, 635
Eco130I CCWWGG 2 cut(s) 178, 245
Eco31I GGTCTC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 1047, 1197
Eco88I CYCGRG 1 cut(s) 144
EcoRI GAATTC 1 cut(s) 1175
EcoRII CCWGG 3 cut(s) 465, 1162, 1246
EcoT14I CCWWGG 2 cut(s) 178, 245
EcoT22I ATGCAT 1 cut(s) 505
ErhI CCWWGG 2 cut(s) 178, 245
FaeI CATG 5 cut(s) 84, 758, 790, 812, 1015
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 5 cut(s) 115, 293, 935, 1000, 1229
FatI CATG 5 cut(s) 80, 754, 786, 808, 1011
FauI CCCGC 1 cut(s) 632
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 2 cut(s) 989, 1201
Fnu4HI GCNGC 5 cut(s) 32, 78, 475, 791, 1313
FokI GGATG 2 cut(s) 794, 1044
Fsp4HI GCNGC 5 cut(s) 32, 78, 475, 791, 1313
GlaI GCGC 1 cut(s) 30
GluI GCNGC 5 cut(s) 32, 78, 475, 791, 1313
GsaI CCCAGC 1 cut(s) 280
GsuI CTGGAG 1 cut(s) 1292
HaeIII GGCC 3 cut(s) 486, 813, 1275
HgaI GACGC 2 cut(s) 73, 1148
HhaI GCGC 1 cut(s) 31
Hin1II CATG 5 cut(s) 84, 758, 790, 812, 1015
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 2 cut(s) 300, 674
HindII GTYRAC 2 cut(s) 300, 674
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 5 cut(s) 65, 119, 264, 623, 887
HphI GGTGA 3 cut(s) 32, 146, 1178
Hpy166II GTNNAC 5 cut(s) 300, 581, 674, 990, 1202
Hpy188I TCNGA 8 cut(s) 101, 115, 349, 575, 604, 863, 982, 1331
Hpy188III TCNNGA 9 cut(s) 146, 314, 379, 655, 755, 848, 881, 968, 1025
Hpy8I GTNNAC 5 cut(s) 300, 581, 674, 990, 1202
Hpy99I CGWCG 1 cut(s) 819
HpyAV CCTTC 5 cut(s) 161, 204, 1022, 1028, 1220
HpyCH4III ACNGT 4 cut(s) 526, 662, 1146, 1299
HpyCH4IV ACGT 2 cut(s) 544, 577
HpyCH4V TGCA 5 cut(s) 34, 344, 503, 762, 1289
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 259, 350, 471, 483
HpyF3I CTNAG 4 cut(s) 44, 67, 98, 556
HpySE526I ACGT 2 cut(s) 544, 577
Hsp92II CATG 5 cut(s) 84, 758, 790, 812, 1015
HspAI GCGC 1 cut(s) 29
KpnI GGTACC 1 cut(s) 690
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 5 cut(s) 18, 89, 461, 777, 1299
LweI GCATC 4 cut(s) 263, 353, 525, 724
MaeII ACGT 2 cut(s) 544, 577
MaeIII GTNAC 3 cut(s) 209, 520, 1190
MalI GATC 8 cut(s) 24, 410, 572, 601, 771, 966, 1007, 1088
MbiI CCGCTC 1 cut(s) 59
MboI GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 7 cut(s) 4, 285, 307, 750, 856, 1072, 1175
MlyI GAGTC 2 cut(s) 59, 273
MmeI TCCRAC 3 cut(s) 425, 725, 1102
Mph1103I ATGCAT 1 cut(s) 505
MseI TTAA 4 cut(s) 234, 306, 837, 1041
MspA1I CMGCKG 1 cut(s) 1312
MspR9I CCNGG 3 cut(s) 467, 1164, 1248
Mva1269I GAATGC 1 cut(s) 1053
MvaI CCWGG 3 cut(s) 467, 1164, 1248
MwoI GCNNNNNNNGC 5 cut(s) 77, 259, 350, 471, 483
NdeII GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
NlaIII CATG 5 cut(s) 84, 758, 790, 812, 1015
NlaIV GGNNCC 4 cut(s) 131, 410, 688, 1263
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 3 cut(s) 84, 790, 1015
PaeI GCATGC 2 cut(s) 84, 790
PaeR7I CTCGAG 1 cut(s) 144
PagI TCATGA 1 cut(s) 754
PciSI GCTCTTC 1 cut(s) 798
PctI GAATGC 1 cut(s) 1053
PfeI GAWTC 3 cut(s) 119, 623, 887
PflMI CCANNNNNTGG 2 cut(s) 707, 1271
PkrI GCNGC 5 cut(s) 33, 79, 476, 792, 1314
PleI GAGTC 2 cut(s) 59, 272
PpsI GAGTC 2 cut(s) 59, 272
PshBI ATTAAT 2 cut(s) 306, 1041
Psp6I CCWGG 3 cut(s) 465, 1162, 1246
PspFI CCCAGC 1 cut(s) 276
PspGI CCWGG 3 cut(s) 465, 1162, 1246
PspN4I GGNNCC 4 cut(s) 131, 410, 688, 1263
PspPI GGNCC 3 cut(s) 129, 484, 1274
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 1 cut(s) 408
PvuII CAGCTG 1 cut(s) 1312
RsaI GTAC 5 cut(s) 631, 664, 688, 729, 1148
RsaNI GTAC 5 cut(s) 630, 663, 687, 728, 1147
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 4 cut(s) 234, 306, 837, 1041
SatI GCNGC 5 cut(s) 32, 78, 475, 791, 1313
Sau3AI GATC 8 cut(s) 22, 408, 570, 599, 769, 964, 1005, 1086
Sau96I GGNCC 3 cut(s) 129, 484, 1274
SchI GAGTC 2 cut(s) 59, 273
ScrFI CCNGG 3 cut(s) 467, 1164, 1248
SduI GDGCHC 1 cut(s) 145
SfaNI GCATC 4 cut(s) 263, 353, 525, 724
Sfr274I CTCGAG 1 cut(s) 144
SinI GGWCC 1 cut(s) 129
SlaI CTCGAG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
SphI GCATGC 2 cut(s) 84, 790
Sse9I AATT 7 cut(s) 4, 285, 307, 750, 856, 1072, 1175
SsiI CCGC 5 cut(s) 59, 108, 620, 639, 1009
StyD4I CCNGG 3 cut(s) 465, 1162, 1246
StyI CCWWGG 2 cut(s) 178, 245
TaaI ACNGT 4 cut(s) 526, 662, 1146, 1299
TaiI ACGT 2 cut(s) 547, 580
TaqI TCGA 3 cut(s) 145, 171, 1004
TasI AATT 7 cut(s) 4, 285, 307, 750, 856, 1072, 1175
TatI WGTACW 2 cut(s) 629, 662
TfiI GAWTC 3 cut(s) 119, 623, 887
Tru1I TTAA 4 cut(s) 234, 306, 837, 1041
Tru9I TTAA 4 cut(s) 234, 306, 837, 1041
TscAI CASTG 5 cut(s) 21, 336, 910, 1088, 1296
TseI GCWGC 5 cut(s) 31, 77, 474, 790, 1312
TspDTI ATGAA 5 cut(s) 17, 132, 413, 581, 743
TspRI CASTG 5 cut(s) 21, 336, 910, 1088, 1296
Van91I CCANNNNNTGG 2 cut(s) 707, 1271
VpaK11BI GGWCC 1 cut(s) 129
VspI ATTAAT 2 cut(s) 306, 1041
XapI RAATTY 3 cut(s) 4, 856, 1175
XceI RCATGY 3 cut(s) 84, 790, 1015
XhoI CTCGAG 1 cut(s) 144
XmiI GTMKAC 2 cut(s) 989, 1201
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.