Rh4BG135800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006360
Physical Location & Seq
Reverse (-)
24135477 .. 24140223
4747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG135800.1

Sequence Viewer

Length: 1332 bp
ATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACACATAATCCTGCGCAAGTTAGGAACTGAAGAGTCAGGGAGAGCCGCCTGCGTCAGCAAGCAACTCAGAGCTTCGGCCTCTGAAGAACCTCTTTGGTCCCATTTCTGTGCCCAGGAGCTCCACCTCTCACAACCCTTGGATCCTCATGGCAACCTTGCTCCTTCTTTCAAGGCATGTTATCAATCATGGCGGGAATCTTTTCATATGTATCCTTGGTCCCTTGTCAAGCGAGTCAAAAGGTGTTGGGGCAAAATTACCAACTGGCTTACTCTTAATTTCCCAGAGGCTCAGTCTACGCTTAACAAAGGTGCATCAGAAGCTGACATTCAAGAGTTGGAGAACACATTGAAAGTAAAACTCCCTCTCCCAACCAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTAGAGGACAAGAATTGGTCAAGTAGCCGAAATGGCACTCTACTGGGTCTCATAGGTGGTTATTCTTTTTATGACCGCATGGTAAATGTCTGCTTGTTGCCTTTACATCACATAGTATTAGCAACAAAGGACATCATGGAAACGCTTGATTTTTCAGCAACACCAGATTATATTGTTGTGGCTGCTTCTACTACACATAGCCAAAAGTTGTTTTTCCTCAACTGTACAAGTGGTCAACTCTATGTTGGTACGGCCAACCTTCTGCTTGATGGTGAAATGCTTCCTTGTGTACCGAATGCGTTGGTTAATTCAGTTCATGATTACAACGGTGATCGACAGCAGGATGCCATGCTTCTTTGGTTGGAAGAACACGGCCGCCGTCTAGAAAATGGAATTATTAAACTCCGTGAACAAGAAACTATGAGAAGCATCTGTCAGTTTCCAGAGGAACCCCCTTTCTGTTCCACTGCCGTAACAAGTGGCGTACAGGTTCGTTGTTCTTCGGTCTTTGTCCCGGAGTTTACTAATCTTCAAGATACCAACCAGAAGTACTTTTTTTCCTATTCAATTCGCATGTCGCTTCTACCTGAAGGATGTAGCATCAATGGAATGACATTCAACTCTTGCCAACTGCTTTCGAGGCACTGGGTCATTCGTGCTGGTGATGTTGTGGTAGGTAATGTAAATGCAGAGGCTGTTATAGGAAAGTACCCTCTCTTGCGTCCAGGTGAGCCAGAATTTGTATATGAGAGTTGCACGCCGCTGCCATCTTTGTCAGGTTCTATAGAAGGTTCTTTTGTGTTTGTCCCGGGCAGGTTGGCGGACCGAAAAGGCAGAGGATTTAGAGTTCTAGCTGCATGCTTTCCCCTCCAGCTGCCAGACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

443

Amino Acids

49.68

Weight (kDa)

6.08

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 324 - 415 1.1e-21 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 1242
AccI GTMKAC 1 cut(s) 326
AciI CCGC 6 cut(s) 78, 223, 514, 814, 1199, 1259
AclWI GGATC 2 cut(s) 167, 180
AcoI YGGCCR 2 cut(s) 690, 811
AcsI RAATTY 1 cut(s) 1175
AcuI CTGAAG 3 cut(s) 81, 135, 1047
AfaI GTAC 6 cut(s) 664, 688, 729, 924, 989, 1148
AfeI AGCGCT 1 cut(s) 30
AgsI TTSAA 6 cut(s) 202, 362, 382, 971, 1005, 1057
AjnI CCWGG 3 cut(s) 144, 404, 1162
AloI GAACNNNNNNTCC 2 cut(s) 1269, 1301
AluBI AGCT 5 cut(s) 104, 151, 353, 1292, 1312
AluI AGCT 5 cut(s) 104, 151, 353, 1292, 1312
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 1 cut(s) 491
AlwI GGATC 2 cut(s) 167, 180
AlwNI CAGNNNCTG 2 cut(s) 353, 1133
Ama87I CYCGRG 1 cut(s) 1246
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 3 cut(s) 108, 690, 811
ApeKI GCWGC 4 cut(s) 620, 1201, 1292, 1312
ApoI RAATTY 1 cut(s) 1175
Asp700I GAANNNNTTC 2 cut(s) 231, 717
AspLEI GCGC 2 cut(s) 31, 48
AspS9I GGNCC 3 cut(s) 129, 249, 1261
AsuC2I CCSGG 3 cut(s) 953, 1247, 1248
AsuHPI GGTGA 5 cut(s) 32, 722, 779, 1112, 1178
AvaI CYCGRG 1 cut(s) 1246
AvaII GGWCC 3 cut(s) 129, 249, 1261
BaeGI GKGCMC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 4 cut(s) 607, 1188, 1279, 1299
BccI CCATC 2 cut(s) 701, 1213
BceAI ACGGC 4 cut(s) 705, 801, 826, 893
BciT130I CCWGG 3 cut(s) 146, 406, 1164
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 3 cut(s) 953, 1247, 1248
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 3 cut(s) 26, 821, 1289
BfmI CTRYAG 1 cut(s) 1221
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 1242
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 471
BisI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
BlsI GCNGC 7 cut(s) 79, 622, 815, 1200, 1203, 1294, 1314
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
Bme18I GGWCC 3 cut(s) 129, 249, 1261
BmeT110I CYCGRG 1 cut(s) 1246
BmgT120I GGNCC 3 cut(s) 129, 249, 1261
BmiI GGNNCC 4 cut(s) 131, 174, 251, 888
BmrFI CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
BmrI ACTGGG 2 cut(s) 491, 1093
BmsI GCATC 4 cut(s) 353, 772, 876, 1047
BmuI ACTGGG 2 cut(s) 491, 1093
BpmI CTGGAG 1 cut(s) 1292
BpuMI CCSGG 3 cut(s) 953, 1247, 1248
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 4 cut(s) 144, 168, 245, 1246
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bse1I ACTGG 3 cut(s) 299, 486, 1088
BseBI CCWGG 3 cut(s) 146, 406, 1164
BseDI CCNNGG 4 cut(s) 144, 168, 245, 1246
BseGI GGATG 2 cut(s) 787, 1037
BseMII CTCAG 2 cut(s) 112, 335
BseNI ACTGG 3 cut(s) 299, 486, 1088
BseSI GKGCMC 1 cut(s) 145
BseX3I CGGCCG 1 cut(s) 811
BseXI GCAGC 4 cut(s) 607, 1188, 1279, 1299
Bsh1285I CGRYCG 1 cut(s) 814
BshFI GGCC 3 cut(s) 110, 692, 813
BsiEI CGRYCG 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 1246
BsiSI CCGG 2 cut(s) 953, 1247
BslFI GGGAC 4 cut(s) 115, 235, 935, 1229
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 4 cut(s) 115, 235, 935, 1229
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 3 cut(s) 110, 692, 813
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 1 cut(s) 1246
Bsp1286I GDGCHC 2 cut(s) 145, 153
Bsp1407I TGTACA 1 cut(s) 662
Bsp143I GATC 3 cut(s) 22, 172, 769
BspACI CCGC 6 cut(s) 78, 223, 514, 814, 1199, 1259
BspANI GGCC 3 cut(s) 110, 692, 813
BspCNI CTCAG 2 cut(s) 111, 334
BspHI TCATGA 2 cut(s) 424, 754
BspLI GGNNCC 4 cut(s) 131, 174, 251, 888
BspMI ACCTGC 1 cut(s) 1242
BspPI GGATC 2 cut(s) 167, 180
BspTNI GGTCTC 1 cut(s) 491
BsrGI TGTACA 1 cut(s) 662
BsrI ACTGG 3 cut(s) 299, 486, 1088
BssECI CCNNGG 4 cut(s) 144, 168, 245, 1246
BssMI GATC 3 cut(s) 22, 172, 769
BssT1I CCWWGG 2 cut(s) 168, 245
Bst2UI CCWGG 3 cut(s) 146, 406, 1164
Bst4CI ACNGT 3 cut(s) 431, 662, 767
Bst6I CTCTTC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 662
BstC8I GCNNGC 4 cut(s) 82, 92, 1196, 1297
BstDEI CTNAG 2 cut(s) 98, 321
BstF5I GGATG 2 cut(s) 787, 1037
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 48
BstKTI GATC 3 cut(s) 25, 175, 772
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 3 cut(s) 22, 172, 769
BstMCI CGRYCG 1 cut(s) 814
BstMWI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
BstNI CCWGG 3 cut(s) 146, 406, 1164
BstNSI RCATGY 3 cut(s) 210, 1015, 1299
BstSCI CCNGG 6 cut(s) 144, 404, 951, 1162, 1245, 1246
BstSFI CTRYAG 1 cut(s) 1221
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 4 cut(s) 607, 1188, 1279, 1299
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BstZI CGGCCG 1 cut(s) 811
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 110, 692, 813
BtsCI GGATG 2 cut(s) 787, 1037
BtsI GCAGTG 2 cut(s) 21, 903
BtsIMutI CAGTG 3 cut(s) 21, 903, 1081
BveI ACCTGC 1 cut(s) 1242
Cac8I GCNNGC 4 cut(s) 82, 92, 1196, 1297
CaiI CAGNNNCTG 2 cut(s) 353, 1133
CciI TCATGA 2 cut(s) 424, 754
CfoI GCGC 2 cut(s) 31, 48
Cfr13I GGNCC 3 cut(s) 129, 249, 1261
Cfr9I CCCGGG 1 cut(s) 1246
CpoI CGGWCCG 1 cut(s) 1261
CseI GACGC 2 cut(s) 73, 1148
Csp6I GTAC 6 cut(s) 663, 687, 728, 923, 988, 1147
CspI CGGWCCG 1 cut(s) 1261
CviQI GTAC 6 cut(s) 663, 687, 728, 923, 988, 1147
DdeI CTNAG 2 cut(s) 98, 321
DpnI GATC 3 cut(s) 24, 174, 771
DpnII GATC 3 cut(s) 22, 172, 769
EaeI YGGCCR 2 cut(s) 690, 811
EagI CGGCCG 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 1274
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 1 cut(s) 811
Eco130I CCWWGG 2 cut(s) 168, 245
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 3 cut(s) 129, 249, 1261
Eco47III AGCGCT 1 cut(s) 30
Eco52I CGGCCG 1 cut(s) 811
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 81, 135, 1047
Eco88I CYCGRG 1 cut(s) 1246
EcoICRI GAGCTC 1 cut(s) 151
EcoRII CCWGG 3 cut(s) 144, 404, 1162
EcoT14I CCWWGG 2 cut(s) 168, 245
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 2 cut(s) 168, 245
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 4 cut(s) 115, 235, 935, 1229
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 237
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
FokI GGATG 2 cut(s) 794, 1044
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
FspBI CTAG 3 cut(s) 26, 821, 1289
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 30, 47
GluI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
GsuI CTGGAG 1 cut(s) 1292
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 3 cut(s) 110, 692, 813
HapII CCGG 2 cut(s) 953, 1247
HgaI GACGC 2 cut(s) 73, 1148
HhaI GCGC 2 cut(s) 31, 48
Hin6I GCGC 2 cut(s) 29, 46
HinP1I GCGC 2 cut(s) 29, 46
HincII GTYRAC 1 cut(s) 674
HindII GTYRAC 1 cut(s) 674
HinfI GANTC 4 cut(s) 65, 227, 264, 409
HpaII CCGG 2 cut(s) 953, 1247
HphI GGTGA 5 cut(s) 32, 722, 779, 1112, 1178
Hpy166II GTNNAC 5 cut(s) 327, 674, 728, 848, 960
Hpy188I TCNGA 4 cut(s) 101, 115, 349, 1331
Hpy188III TCNNGA 7 cut(s) 362, 425, 434, 755, 821, 881, 971
Hpy8I GTNNAC 5 cut(s) 327, 674, 728, 848, 960
HpyAV CCTTC 4 cut(s) 204, 707, 1022, 1220
HpyCH4III ACNGT 3 cut(s) 431, 662, 767
HpyCH4V TGCA 4 cut(s) 344, 1127, 1194, 1295
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
HpyF3I CTNAG 2 cut(s) 98, 321
HspAI GCGC 2 cut(s) 29, 46
Kzo9I GATC 3 cut(s) 22, 172, 769
LmnI GCTCC 3 cut(s) 148, 156, 196
Lsp1109I GCAGC 4 cut(s) 607, 1188, 1279, 1299
LweI GCATC 4 cut(s) 353, 772, 876, 1047
MaeI CTAG 3 cut(s) 26, 821, 1289
MaeIII GTNAC 1 cut(s) 910
MalI GATC 3 cut(s) 24, 174, 771
MboI GATC 3 cut(s) 22, 172, 769
MboII GAAGA 6 cut(s) 74, 128, 815, 930, 959, 1318
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 145, 153
MluCI AATT 7 cut(s) 285, 307, 451, 745, 831, 1005, 1175
MlyI GAGTC 2 cut(s) 74, 273
MmeI TCCRAC 2 cut(s) 348, 780
MroXI GAANNNNTTC 2 cut(s) 231, 717
MseI TTAA 4 cut(s) 306, 333, 744, 837
MslI CAYNNNNRTG 1 cut(s) 138
MspA1I CMGCKG 2 cut(s) 1201, 1312
MspI CCGG 2 cut(s) 953, 1247
MspR9I CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 146, 406, 1164
MwoI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
NciI CCSGG 3 cut(s) 953, 1247, 1248
NdeI CATATG 1 cut(s) 237
NdeII GATC 3 cut(s) 22, 172, 769
NlaIV GGNNCC 4 cut(s) 131, 174, 251, 888
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 3 cut(s) 210, 1015, 1299
PaeI GCATGC 1 cut(s) 1299
PagI TCATGA 2 cut(s) 424, 754
PctI GAATGC 1 cut(s) 739
PdmI GAANNNNTTC 2 cut(s) 231, 717
PfeI GAWTC 2 cut(s) 227, 409
PfoI TCCNGGA 1 cut(s) 951
PkrI GCNGC 7 cut(s) 79, 622, 815, 1200, 1203, 1294, 1314
PleI GAGTC 2 cut(s) 73, 272
PpsI GAGTC 2 cut(s) 73, 272
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 144, 404, 1162
PspGI CCWGG 3 cut(s) 144, 404, 1162
PspN4I GGNNCC 4 cut(s) 131, 174, 251, 888
PspPI GGNCC 3 cut(s) 129, 249, 1261
PstNI CAGNNNCTG 2 cut(s) 353, 1133
PsuI RGATCY 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 1312
RsaI GTAC 6 cut(s) 664, 688, 729, 924, 989, 1148
RsaNI GTAC 6 cut(s) 663, 687, 728, 923, 988, 1147
RseI CAYNNNNRTG 1 cut(s) 138
Rsr2I CGGWCCG 1 cut(s) 1261
RsrII CGGWCCG 1 cut(s) 1261
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 4 cut(s) 306, 333, 744, 837
SatI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
Sau3AI GATC 3 cut(s) 22, 172, 769
Sau96I GGNCC 3 cut(s) 129, 249, 1261
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 2 cut(s) 74, 273
ScrFI CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
SduI GDGCHC 2 cut(s) 145, 153
SfaNI GCATC 4 cut(s) 353, 772, 876, 1047
SfcI CTRYAG 1 cut(s) 1221
SinI GGWCC 3 cut(s) 129, 249, 1261
SmaI CCCGGG 1 cut(s) 1248
SmiMI CAYNNNNRTG 1 cut(s) 138
SphI GCATGC 1 cut(s) 1299
Sse9I AATT 7 cut(s) 285, 307, 451, 745, 831, 1005, 1175
SsiI CCGC 6 cut(s) 78, 223, 514, 814, 1199, 1259
SspMI CTAG 3 cut(s) 26, 821, 1289
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 6 cut(s) 144, 404, 951, 1162, 1245, 1246
StyI CCWWGG 2 cut(s) 168, 245
TaaI ACNGT 3 cut(s) 431, 662, 767
TaqI TCGA 2 cut(s) 772, 1076
TaqII GACCGA 2 cut(s) 931, 1278
TasI AATT 7 cut(s) 285, 307, 451, 745, 831, 1005, 1175
TatI WGTACW 2 cut(s) 662, 987
TauI GCSGC 3 cut(s) 80, 816, 1201
TfiI GAWTC 2 cut(s) 227, 409
Tru1I TTAA 4 cut(s) 306, 333, 744, 837
Tru9I TTAA 4 cut(s) 306, 333, 744, 837
TscAI CASTG 3 cut(s) 21, 910, 1088
TseI GCWGC 4 cut(s) 620, 1201, 1292, 1312
TspDTI ATGAA 3 cut(s) 224, 413, 743
TspGWI ACGGA 1 cut(s) 833
TspMI CCCGGG 1 cut(s) 1246
TspRI CASTG 3 cut(s) 21, 910, 1088
VpaK11BI GGWCC 3 cut(s) 129, 249, 1261
XapI RAATTY 1 cut(s) 1175
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 3 cut(s) 210, 1015, 1299
XmaI CCCGGG 1 cut(s) 1246
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 2 cut(s) 231, 717
XspI CTAG 3 cut(s) 26, 821, 1289
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.