Rorug04G0071800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006360
Physical Location & Seq
Reverse (-)
11037487 .. 11039873
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0071800.1

Sequence Viewer

Length: 1350 bp
ATGCTTTCTTCCCTAACCTCTCAACGCCTCAGACCCTTTCTTCTTCTTCATCTCTCCCACACTCAAACCCTCCTATTCTCCTCACTTCCTCACACTTCTCCCACCAAACCCATTTCAATTCCCAATCCCACAGACCCAAATCCTCAATCAACTTCACCAATTTACGACTACACCACAGTGCGTGAAACCCTTTCTTCTTACTGCAATGACTGGAAGAAAGCCCTCGACTTTTTCAACTGGGTTGAAACCCAGTACCACTTCCAACACACCACCGAAACCTACAATCGAATGCTTGACATTTTCGGTAAGTACTTCGAGTTTGAGCTTTCTTGGGACTTGGTTCACAAAATGAAACAGAACCCAAGTTCTGTTCCTGACCATACTACATTTCGTATAATGTTTAAGAGATATGTGTCTGCACATCTTGTAAAGGAAGCTATAGATACTTATAATAAGTTGGATGAGTTTAGTTTGAAGGATGAGACTTCTTATTGCAATCTTATTGATGCTCTTTGTGAGCATAAGCATGTGATTGAGGCCCAAGAGCTTTGCTCTTGGAAGAACAAAGAGTTGGGTTTTGATAAGAGTACTAAGCTGCATAATATAATTCTTCGGGGGTGGTCGAAAATGGGGTGGTGGGGAAAGTGTAGGGATTTTTGGGAAGAGATGGATAGGAGAGGTGTTTGTAAAGATTTGCATTCGTATTCGATATATATGGATATAATGTGTAAGAGTGGGAAGGCGTGGAAGGCTGTGAAGTTGTATAAGGAGGTGAAGAGGAAAGGGATCAAGCTGGATGTGGTGGCGTATAATACGGTTATTCGTGCTGTTGGTGATTCTGAGGGTGTTGATTTTGCAACTCGGATCTTGAGGGAGATGAAGGAATTGGGATGTGAACCTAATACTGCGACGTATAATACAATTATAAAGCTTTTGTGTGAGAATATGAGATTCAGGGAGGCATTTTCAATGCTTCGAATAATGTCTAAGAACAGTTGTGGCCCGGATGTTATTACGTACCAAATTCTTTTTAAGTATCTGGAGAAGCCGAATGAGATTCTTAGGCTGTTTGATAGGATGATTGAAAGTGGGGTTCAGCCGAGGATGGACACATATGTGATGATTATGAGGAAGTTTGGGAGATGGGGCTTCCTTAGACCGATGTTTATTGTGTGGCAGAAGATGGAAAAACTTGGGTGTAGTCCTAATGAGTCTGCTTACAATGCTTTAATAGATGCTCTGGTTGAAAAGGGTATGTTAGACATGGCTAGGAAGTACAATGAAGAGATGATAGCAAAGGGCCTTCCAGCTAGGCCAAGGGTGGAGTTGGGCACAAAACTGGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

449

Amino Acids

52.68

Weight (kDa)

8.83

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 231 - 276 4.4e-09 PPR repeat family
PPR_long PF17177 238 - 368 1.2e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 254 - 312 8.1e-15 Pentatricopeptide repeat domain
PPR_2 PF13041 266 - 313 3.2e-12 PPR repeat family
TPR_24 PF23276 283 - 355 9.6e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 297 - 328 2.4e-09 PPR repeat
PPR_2 PF13041 300 - 346 9.8e-14 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 450, 926
AclWI GGATC 2 cut(s) 794, 872
AcsI RAATTY 1 cut(s) 1023
AfaI GTAC 5 cut(s) 254, 311, 589, 1019, 1277
AgsI TTSAA 7 cut(s) 117, 235, 245, 475, 969, 1085, 1247
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AleI CACNNNNGTG 2 cut(s) 176, 1115
AluBI AGCT 7 cut(s) 325, 437, 547, 595, 793, 931, 1310
AluI AGCT 7 cut(s) 325, 437, 547, 595, 793, 931, 1310
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 2 cut(s) 794, 872
AoxI GGCC 4 cut(s) 537, 1000, 1300, 1313
ApeKI GCWGC 1 cut(s) 595
ApoI RAATTY 1 cut(s) 1023
AspS9I GGNCC 3 cut(s) 538, 1001, 1300
AsuC2I CCSGG 1 cut(s) 1004
AsuHPI GGTGA 3 cut(s) 147, 784, 845
AsuII TTCGAA 1 cut(s) 976
BaeGI GKGCMC 1 cut(s) 1334
BbvI GCAGC 1 cut(s) 582
BccI CCATC 4 cut(s) 661, 1099, 1137, 1177
BcnI CCSGG 1 cut(s) 1004
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 2 cut(s) 1269, 1311
BfmI CTRYAG 1 cut(s) 438
BisI GCNGC 1 cut(s) 596
BlsI GCNGC 1 cut(s) 597
BmcAI AGTACT 2 cut(s) 311, 589
Bme1390I CCNGG 1 cut(s) 1004
BmgT120I GGNCC 3 cut(s) 538, 1001, 1300
BmrFI CCNGG 1 cut(s) 1004
BmrI ACTGGG 2 cut(s) 244, 247
BmsI GCATC 2 cut(s) 496, 1225
BmuI ACTGGG 2 cut(s) 244, 247
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpmI CTGGAG 1 cut(s) 1061
Bpu14I TTCGAA 1 cut(s) 976
BpuEI CTTGAG 1 cut(s) 889
BpuMI CCSGG 1 cut(s) 1004
BsaAI YACGTR 1 cut(s) 1017
BsaJI CCNNGG 2 cut(s) 1100, 1316
Bse1I ACTGG 4 cut(s) 215, 242, 250, 1344
Bse3DI GCAATG 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 1100, 1316
BseGI GGATG 7 cut(s) 466, 484, 802, 896, 1012, 1083, 1110
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 43, 831
BseNI ACTGG 4 cut(s) 215, 242, 250, 1344
BseRI GAGGAG 1 cut(s) 70
BseSI GKGCMC 1 cut(s) 1334
BseXI GCAGC 1 cut(s) 582
BsgI GTGCAG 1 cut(s) 402
BshFI GGCC 4 cut(s) 539, 1002, 1302, 1315
BsiSI CCGG 1 cut(s) 1004
BslFI GGGAC 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 347
BsmI GAATGC 2 cut(s) 294, 697
BsnI GGCC 4 cut(s) 539, 1002, 1302, 1315
Bsp119I TTCGAA 1 cut(s) 976
Bsp1286I GDGCHC 1 cut(s) 1334
Bsp143I GATC 2 cut(s) 786, 864
BspANI GGCC 4 cut(s) 539, 1002, 1302, 1315
BspCNI CTCAG 2 cut(s) 42, 832
BspPI GGATC 2 cut(s) 794, 872
BspT104I TTCGAA 1 cut(s) 976
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 4 cut(s) 215, 242, 250, 1344
BssECI CCNNGG 2 cut(s) 1100, 1316
BssMI GATC 2 cut(s) 786, 864
BssT1I CCWWGG 1 cut(s) 1316
Bst4CI ACNGT 3 cut(s) 178, 817, 995
Bst6I CTCTTC 3 cut(s) 657, 770, 1278
BstBAI YACGTR 1 cut(s) 1017
BstBI TTCGAA 1 cut(s) 976
BstDEI CTNAG 6 cut(s) 29, 591, 840, 987, 1061, 1154
BstF5I GGATG 7 cut(s) 466, 484, 802, 896, 1012, 1083, 1110
BstKTI GATC 2 cut(s) 789, 867
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 2 cut(s) 786, 864
BstMWI GCNNNNNNNGC 2 cut(s) 749, 1223
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 1 cut(s) 1002
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 1334
BstSNI TACGTA 1 cut(s) 1017
BstV1I GCAGC 1 cut(s) 582
BstX2I RGATCY 1 cut(s) 864
BstYI RGATCY 1 cut(s) 864
BsuRI GGCC 4 cut(s) 539, 1002, 1302, 1315
BtsCI GGATG 7 cut(s) 466, 484, 802, 896, 1012, 1083, 1110
BtsIMutI CAGTG 1 cut(s) 183
Cfr13I GGNCC 3 cut(s) 538, 1001, 1300
Csp6I GTAC 5 cut(s) 253, 310, 588, 1018, 1276
CspCI CAANNNNNGTGG 1 cut(s) 1323
CviAII CATG 2 cut(s) 527, 1264
CviQI GTAC 5 cut(s) 253, 310, 588, 1018, 1276
DdeI CTNAG 6 cut(s) 29, 591, 840, 987, 1061, 1154
DpnI GATC 2 cut(s) 788, 866
DpnII GATC 2 cut(s) 786, 864
Eam1104I CTCTTC 3 cut(s) 657, 770, 1278
EarI CTCTTC 3 cut(s) 657, 770, 1278
Eco105I TACGTA 1 cut(s) 1017
Eco130I CCWWGG 1 cut(s) 1316
EcoO109I RGGNCCY 1 cut(s) 1300
EcoT14I CCWWGG 1 cut(s) 1316
ErhI CCWWGG 1 cut(s) 1316
FaeI CATG 2 cut(s) 530, 1267
FaqI GGGAC 1 cut(s) 347
FatI CATG 2 cut(s) 526, 1263
FauNDI CATATG 1 cut(s) 1114
Fnu4HI GCNGC 1 cut(s) 596
FokI GGATG 7 cut(s) 473, 491, 809, 903, 1019, 1090, 1117
Fsp4HI GCNGC 1 cut(s) 596
FspBI CTAG 2 cut(s) 1269, 1311
GluI GCNGC 1 cut(s) 596
GsuI CTGGAG 1 cut(s) 1061
HaeIII GGCC 4 cut(s) 539, 1002, 1302, 1315
HapII CCGG 1 cut(s) 1004
Hin1II CATG 2 cut(s) 530, 1267
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 4 cut(s) 836, 951, 1057, 1211
HpaII CCGG 1 cut(s) 1004
HphI GGTGA 3 cut(s) 147, 784, 845
Hpy166II GTNNAC 2 cut(s) 343, 896
Hpy188I TCNGA 3 cut(s) 32, 841, 864
Hpy188III TCNNGA 3 cut(s) 374, 868, 1040
Hpy8I GTNNAC 2 cut(s) 343, 896
Hpy99I CGWCG 1 cut(s) 913
HpyAV CCTTC 5 cut(s) 469, 733, 742, 874, 1313
HpyCH4III ACNGT 3 cut(s) 178, 817, 995
HpyCH4IV ACGT 2 cut(s) 911, 1016
HpyCH4V TGCA 6 cut(s) 204, 419, 495, 598, 697, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 749, 1223
HpyF3I CTNAG 6 cut(s) 29, 591, 840, 987, 1061, 1154
HpySE526I ACGT 2 cut(s) 911, 1016
Hsp92II CATG 2 cut(s) 530, 1267
Kzo9I GATC 2 cut(s) 786, 864
Lsp1109I GCAGC 1 cut(s) 582
LweI GCATC 2 cut(s) 496, 1225
MaeI CTAG 2 cut(s) 1269, 1311
MaeII ACGT 2 cut(s) 911, 1016
MalI GATC 2 cut(s) 788, 866
MboI GATC 2 cut(s) 786, 864
MflI RGATCY 1 cut(s) 864
MhlI GDGCHC 1 cut(s) 1334
MluCI AATT 6 cut(s) 117, 159, 606, 884, 921, 1023
MlyI GAGTC 1 cut(s) 1220
MmeI TCCRAC 2 cut(s) 286, 438
MseI TTAA 3 cut(s) 402, 1032, 1229
MslI CAYNNNNRTG 3 cut(s) 176, 525, 1115
MspI CCGG 1 cut(s) 1004
MspR9I CCNGG 1 cut(s) 1004
Mva1269I GAATGC 2 cut(s) 294, 697
MwoI GCNNNNNNNGC 2 cut(s) 749, 1223
NciI CCSGG 1 cut(s) 1004
NdeI CATATG 1 cut(s) 1114
NdeII GATC 2 cut(s) 786, 864
NlaIII CATG 2 cut(s) 530, 1267
NmeAIII GCCGAG 1 cut(s) 1125
NspI RCATGY 1 cut(s) 530
NspV TTCGAA 1 cut(s) 976
OliI CACNNNNGTG 2 cut(s) 176, 1115
PctI GAATGC 2 cut(s) 294, 697
PfeI GAWTC 3 cut(s) 836, 951, 1057
PkrI GCNGC 1 cut(s) 597
PleI GAGTC 1 cut(s) 1219
PpsI GAGTC 1 cut(s) 1219
Ppu21I YACGTR 1 cut(s) 1017
PsiI TTATAA 2 cut(s) 450, 926
PspPI GGNCC 3 cut(s) 538, 1001, 1300
PsuI RGATCY 1 cut(s) 864
RsaI GTAC 5 cut(s) 254, 311, 589, 1019, 1277
RsaNI GTAC 5 cut(s) 253, 310, 588, 1018, 1276
RseI CAYNNNNRTG 3 cut(s) 176, 525, 1115
SaqAI TTAA 3 cut(s) 402, 1032, 1229
SatI GCNGC 1 cut(s) 596
Sau3AI GATC 2 cut(s) 786, 864
Sau96I GGNCC 3 cut(s) 538, 1001, 1300
ScaI AGTACT 2 cut(s) 311, 589
SchI GAGTC 1 cut(s) 1220
ScrFI CCNGG 1 cut(s) 1004
SduI GDGCHC 1 cut(s) 1334
SfaNI GCATC 2 cut(s) 496, 1225
SfcI CTRYAG 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 976
SmiMI CAYNNNNRTG 3 cut(s) 176, 525, 1115
SmlI CTYRAG 1 cut(s) 868
SmoI CTYRAG 1 cut(s) 868
SnaBI TACGTA 1 cut(s) 1017
Sse9I AATT 6 cut(s) 117, 159, 606, 884, 921, 1023
SspMI CTAG 2 cut(s) 1269, 1311
StyD4I CCNGG 1 cut(s) 1002
StyI CCWWGG 1 cut(s) 1316
TaaI ACNGT 3 cut(s) 178, 817, 995
TaiI ACGT 2 cut(s) 914, 1019
TaqI TCGA 6 cut(s) 225, 286, 315, 623, 707, 976
TaqII GACCGA 1 cut(s) 1174
TasI AATT 6 cut(s) 117, 159, 606, 884, 921, 1023
TatI WGTACW 3 cut(s) 309, 587, 1275
TfiI GAWTC 3 cut(s) 836, 951, 1057
Tru1I TTAA 3 cut(s) 402, 1032, 1229
Tru9I TTAA 3 cut(s) 402, 1032, 1229
TscAI CASTG 1 cut(s) 183
TseI GCWGC 1 cut(s) 595
TspDTI ATGAA 4 cut(s) 38, 365, 893, 1296
TspRI CASTG 1 cut(s) 183
XapI RAATTY 1 cut(s) 1023
XceI RCATGY 1 cut(s) 530
XspI CTAG 2 cut(s) 1269, 1311
ZrmI AGTACT 2 cut(s) 311, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.