Rh4CG146100

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006360
Physical Location & Seq
Reverse (-)
32162242 .. 32167973
5732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG146100.1

Sequence Viewer

Length: 1332 bp
ATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACACATAATCCTGCGCAAGTTAGGAGCTGAAGAGTCAGGGAGAGCCGCCTGCGTCAGCAAGCAACTCAGAGCTTCGGCCTCTGAAGAACCTCTTTGGTCCCATTTCTGTGCCCAGGAGCTCCACCTCTCGCAACCCTTGGATCCTCATGGCAACCTTGCTCCTTCTTTCAAGGCATGTTATCAATCATGGCGGGAATCTTTTCATATGTATCCTTGGTCCCTTGTCAAGCGAGTCAAAAGGTGTTGGGGCAAAATTACCAACTGGCTGACTCTTAATTTCCCAGAGGCTCAGTCTACGCTTAACAAAGGTGCATCAGAAGCTGACATTCAAGATTTGGAGAACAAATTGAAAGTAAAACTTCCCCTCCCAACCAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTAGAGGACAAGAATTGGTCAAGTAGCCGAAATGGCACTCTACTGGGCCTCATAGGTGGTTATTCTTTTTATGACCGCATGGTTAATGTCTGCTTGTTGCCTTTACATCGGATAGTATTAGCAACAAAGGACATCATGGAAACGCTTGATTTTTCAGCAACACCGGATTATATTGTTGTGGCTGCTTCTACATTTAGCCAAAAGTTGTTTTTCCTCAACTGTACAAGTGGTCAACTCTATGTTGGTATGGCCAACCTTCTGCTTGATGGTGAAATGCTTCCTTGTGTACCGAATGCGTTGGTTAATTCAGTTCATGATTACAATGGTGGTGATCGACAGCAGGATGCCATGCTTCTTTGGTTGGAAGAACACGGCCGCCGTCTAGAAAATGGAATTATTAAACTCCGTGAACAAGAAACTTTGAGAAGCATCTGTCAGTTTCCAGAGGAACCCCCTTTCTGTTCCACTGCCGTAACAAGTGGCGTACAGGTTCGTTGTTCTTCGGTCTTTGTCCCGGAGTTTACTAATTTTCAAGATACCATCAGGAAGTACTTTTTTTCCTATTCAATTCGCATGTCGCTTGTACCTGAAGGATGTAGCATCAATGGAATGACATTCAACTCTTGCCAACTGCTTTCAAGGCACTGGGTCATTCGTGCTGGTGATGTTGTGGTAGGTAATGTAGATGCAGAGGCTGTTATAGGAAAGTACCCTCTCTTGCATCCTGGTGAGCCAGAATTTGTATATGAGAGTTGCACGCCGCTGCCATCTTTGTCAGGTTCTATAGAAGGTTCTTTTGTGTTTGTCCCGGGCAGGTTGGCGGACCCAAAAGGCAGAGGATTTGAAGTTCTAGCTGCACGCTTTCCCCTCCAGCTGCCAGACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

443

Amino Acids

49.66

Weight (kDa)

5.92

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 324 - 415 1.2e-20 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 1242
AccI GTMKAC 1 cut(s) 326
AciI CCGC 6 cut(s) 78, 223, 514, 814, 1199, 1259
AclWI GGATC 2 cut(s) 167, 180
AcoI YGGCCR 2 cut(s) 687, 811
AcsI RAATTY 1 cut(s) 1175
AcuI CTGAAG 3 cut(s) 81, 135, 1047
AfaI GTAC 6 cut(s) 661, 726, 924, 989, 1023, 1148
AfeI AGCGCT 1 cut(s) 30
AgsI TTSAA 8 cut(s) 202, 362, 382, 971, 1005, 1057, 1077, 1283
AjnI CCWGG 3 cut(s) 144, 404, 1162
AloI GAACNNNNNNTCC 2 cut(s) 1269, 1301
AluBI AGCT 6 cut(s) 59, 104, 151, 353, 1292, 1312
AluI AGCT 6 cut(s) 59, 104, 151, 353, 1292, 1312
Alw21I GWGCWC 1 cut(s) 153
AlwI GGATC 2 cut(s) 167, 180
AlwNI CAGNNNCTG 2 cut(s) 353, 1133
Ama87I CYCGRG 1 cut(s) 1246
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 4 cut(s) 108, 484, 687, 811
ApeKI GCWGC 4 cut(s) 620, 1201, 1292, 1312
ApoI RAATTY 1 cut(s) 1175
Asp700I GAANNNNTTC 2 cut(s) 231, 714
AspLEI GCGC 2 cut(s) 31, 48
AspS9I GGNCC 4 cut(s) 129, 249, 484, 1261
AsuC2I CCSGG 3 cut(s) 953, 1247, 1248
AsuHPI GGTGA 5 cut(s) 32, 719, 779, 1112, 1178
AvaI CYCGRG 1 cut(s) 1246
AvaII GGWCC 3 cut(s) 129, 249, 1261
BaeGI GKGCMC 1 cut(s) 145
BalI TGGCCA 1 cut(s) 689
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 4 cut(s) 607, 1188, 1279, 1299
BccI CCATC 3 cut(s) 698, 986, 1213
BceAI ACGGC 3 cut(s) 801, 826, 893
BciT130I CCWGG 3 cut(s) 146, 406, 1164
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 3 cut(s) 953, 1247, 1248
BfaI CTAG 3 cut(s) 26, 821, 1289
BfmI CTRYAG 1 cut(s) 1221
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 1242
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 471
BisI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
BlsI GCNGC 7 cut(s) 79, 622, 815, 1200, 1203, 1294, 1314
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
Bme18I GGWCC 3 cut(s) 129, 249, 1261
BmeT110I CYCGRG 1 cut(s) 1246
BmgT120I GGNCC 4 cut(s) 129, 249, 484, 1261
BmiI GGNNCC 5 cut(s) 131, 174, 251, 888, 1263
BmrFI CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
BmrI ACTGGG 2 cut(s) 491, 1093
BmsI GCATC 6 cut(s) 353, 772, 876, 1047, 1114, 1168
BmuI ACTGGG 2 cut(s) 491, 1093
BpmI CTGGAG 1 cut(s) 1292
BpuMI CCSGG 3 cut(s) 953, 1247, 1248
BsaJI CCNNGG 4 cut(s) 144, 168, 245, 1246
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bse1I ACTGG 3 cut(s) 299, 486, 1088
BseBI CCWGG 3 cut(s) 146, 406, 1164
BseDI CCNNGG 4 cut(s) 144, 168, 245, 1246
BseGI GGATG 3 cut(s) 787, 1037, 1159
BseMII CTCAG 2 cut(s) 112, 335
BseNI ACTGG 3 cut(s) 299, 486, 1088
BseSI GKGCMC 1 cut(s) 145
BseX3I CGGCCG 1 cut(s) 811
BseXI GCAGC 4 cut(s) 607, 1188, 1279, 1299
BsgI GTGCAG 1 cut(s) 1278
Bsh1285I CGRYCG 1 cut(s) 814
BshFI GGCC 4 cut(s) 110, 486, 689, 813
BsiEI CGRYCG 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 1246
BsiSI CCGG 3 cut(s) 602, 953, 1247
BslFI GGGAC 4 cut(s) 115, 235, 935, 1229
BsmFI GGGAC 4 cut(s) 115, 235, 935, 1229
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 4 cut(s) 110, 486, 689, 813
BsoBI CYCGRG 1 cut(s) 1246
Bsp1286I GDGCHC 2 cut(s) 145, 153
Bsp1407I TGTACA 1 cut(s) 659
Bsp143I GATC 3 cut(s) 22, 172, 769
BspACI CCGC 6 cut(s) 78, 223, 514, 814, 1199, 1259
BspANI GGCC 4 cut(s) 110, 486, 689, 813
BspCNI CTCAG 2 cut(s) 111, 334
BspHI TCATGA 2 cut(s) 424, 751
BspLI GGNNCC 5 cut(s) 131, 174, 251, 888, 1263
BspMI ACCTGC 1 cut(s) 1242
BspPI GGATC 2 cut(s) 167, 180
BsrGI TGTACA 1 cut(s) 659
BsrI ACTGG 3 cut(s) 299, 486, 1088
BssECI CCNNGG 4 cut(s) 144, 168, 245, 1246
BssMI GATC 3 cut(s) 22, 172, 769
BssT1I CCWWGG 2 cut(s) 168, 245
Bst2UI CCWGG 3 cut(s) 146, 406, 1164
Bst4CI ACNGT 2 cut(s) 431, 659
Bst6I CTCTTC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 659
BstC8I GCNNGC 4 cut(s) 82, 92, 1196, 1297
BstDEI CTNAG 2 cut(s) 98, 321
BstF5I GGATG 3 cut(s) 787, 1037, 1159
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 48
BstKTI GATC 3 cut(s) 25, 175, 772
BstMBI GATC 3 cut(s) 22, 172, 769
BstMCI CGRYCG 1 cut(s) 814
BstMWI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
BstNI CCWGG 3 cut(s) 146, 406, 1164
BstNSI RCATGY 2 cut(s) 210, 1015
BstSCI CCNGG 6 cut(s) 144, 404, 951, 1162, 1245, 1246
BstSFI CTRYAG 1 cut(s) 1221
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 4 cut(s) 607, 1188, 1279, 1299
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BstZI CGGCCG 1 cut(s) 811
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 4 cut(s) 110, 486, 689, 813
BtsCI GGATG 3 cut(s) 787, 1037, 1159
BtsI GCAGTG 2 cut(s) 21, 903
BtsIMutI CAGTG 3 cut(s) 21, 903, 1081
BveI ACCTGC 1 cut(s) 1242
Cac8I GCNNGC 4 cut(s) 82, 92, 1196, 1297
CaiI CAGNNNCTG 2 cut(s) 353, 1133
CciI TCATGA 2 cut(s) 424, 751
CfoI GCGC 2 cut(s) 31, 48
Cfr13I GGNCC 4 cut(s) 129, 249, 484, 1261
Cfr9I CCCGGG 1 cut(s) 1246
CseI GACGC 1 cut(s) 73
Csp6I GTAC 6 cut(s) 660, 725, 923, 988, 1022, 1147
CviAII CATG 9 cut(s) 179, 207, 219, 425, 517, 574, 752, 787, 1012
CviQI GTAC 6 cut(s) 660, 725, 923, 988, 1022, 1147
DdeI CTNAG 2 cut(s) 98, 321
DpnI GATC 3 cut(s) 24, 174, 771
DpnII GATC 3 cut(s) 22, 172, 769
EaeI YGGCCR 2 cut(s) 687, 811
EagI CGGCCG 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 1274
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 1 cut(s) 811
Eco130I CCWWGG 2 cut(s) 168, 245
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 3 cut(s) 129, 249, 1261
Eco47III AGCGCT 1 cut(s) 30
Eco52I CGGCCG 1 cut(s) 811
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 81, 135, 1047
Eco88I CYCGRG 1 cut(s) 1246
EcoICRI GAGCTC 1 cut(s) 151
EcoRII CCWGG 3 cut(s) 144, 404, 1162
EcoT14I CCWWGG 2 cut(s) 168, 245
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 2 cut(s) 168, 245
FaeI CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 790, 1015
FalI AAGNNNNNCTT 4 cut(s) 108, 140, 375, 407
FaqI GGGAC 4 cut(s) 115, 235, 935, 1229
FatI CATG 9 cut(s) 178, 206, 218, 424, 516, 573, 751, 786, 1011
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 237
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
FokI GGATG 3 cut(s) 794, 1044, 1146
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
FspBI CTAG 3 cut(s) 26, 821, 1289
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 30, 47
GluI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
GsuI CTGGAG 1 cut(s) 1292
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 4 cut(s) 110, 486, 689, 813
HapII CCGG 3 cut(s) 602, 953, 1247
HgaI GACGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 31, 48
Hin1II CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 790, 1015
Hin6I GCGC 2 cut(s) 29, 46
HinP1I GCGC 2 cut(s) 29, 46
HincII GTYRAC 1 cut(s) 671
HindII GTYRAC 1 cut(s) 671
HinfI GANTC 5 cut(s) 65, 227, 264, 301, 409
HpaII CCGG 3 cut(s) 602, 953, 1247
HphI GGTGA 5 cut(s) 32, 719, 779, 1112, 1178
Hpy166II GTNNAC 5 cut(s) 327, 671, 725, 848, 960
Hpy188I TCNGA 5 cut(s) 101, 115, 349, 549, 1331
Hpy188III TCNNGA 8 cut(s) 362, 425, 434, 752, 821, 881, 971, 982
Hpy8I GTNNAC 5 cut(s) 327, 671, 725, 848, 960
HpyAV CCTTC 4 cut(s) 204, 704, 1022, 1220
HpyCH4III ACNGT 2 cut(s) 431, 659
HpyCH4V TGCA 5 cut(s) 344, 1127, 1159, 1194, 1295
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
HpyF3I CTNAG 2 cut(s) 98, 321
Hsp92II CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 790, 1015
HspAI GCGC 2 cut(s) 29, 46
Kzo9I GATC 3 cut(s) 22, 172, 769
LmnI GCTCC 4 cut(s) 56, 148, 156, 196
Lsp1109I GCAGC 4 cut(s) 607, 1188, 1279, 1299
LweI GCATC 6 cut(s) 353, 772, 876, 1047, 1114, 1168
MaeI CTAG 3 cut(s) 26, 821, 1289
MaeIII GTNAC 1 cut(s) 910
MalI GATC 3 cut(s) 24, 174, 771
MboI GATC 3 cut(s) 22, 172, 769
MboII GAAGA 5 cut(s) 74, 128, 815, 930, 1318
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 145, 153
MlsI TGGCCA 1 cut(s) 689
MluCI AATT 9 cut(s) 285, 307, 377, 451, 742, 831, 964, 1005, 1175
MluNI TGGCCA 1 cut(s) 689
MlyI GAGTC 3 cut(s) 74, 273, 295
MmeI TCCRAC 1 cut(s) 780
Mox20I TGGCCA 1 cut(s) 689
MroXI GAANNNNTTC 2 cut(s) 231, 714
MscI TGGCCA 1 cut(s) 689
MseI TTAA 5 cut(s) 306, 333, 522, 741, 837
MslI CAYNNNNRTG 2 cut(s) 138, 1164
Msp20I TGGCCA 1 cut(s) 689
MspA1I CMGCKG 2 cut(s) 1201, 1312
MspI CCGG 3 cut(s) 602, 953, 1247
MspR9I CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 3 cut(s) 146, 406, 1164
MwoI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
NciI CCSGG 3 cut(s) 953, 1247, 1248
NdeI CATATG 1 cut(s) 237
NdeII GATC 3 cut(s) 22, 172, 769
NlaIII CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 790, 1015
NlaIV GGNNCC 5 cut(s) 131, 174, 251, 888, 1263
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 2 cut(s) 210, 1015
PagI TCATGA 2 cut(s) 424, 751
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 2 cut(s) 231, 714
PfeI GAWTC 2 cut(s) 227, 409
PfoI TCCNGGA 1 cut(s) 951
PkrI GCNGC 7 cut(s) 79, 622, 815, 1200, 1203, 1294, 1314
PleI GAGTC 3 cut(s) 73, 272, 295
PpsI GAGTC 3 cut(s) 73, 272, 295
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 144, 404, 1162
PspGI CCWGG 3 cut(s) 144, 404, 1162
PspN4I GGNNCC 5 cut(s) 131, 174, 251, 888, 1263
PspPI GGNCC 4 cut(s) 129, 249, 484, 1261
PstNI CAGNNNCTG 2 cut(s) 353, 1133
PsuI RGATCY 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 1312
RsaI GTAC 6 cut(s) 661, 726, 924, 989, 1023, 1148
RsaNI GTAC 6 cut(s) 660, 725, 923, 988, 1022, 1147
RseI CAYNNNNRTG 2 cut(s) 138, 1164
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 5 cut(s) 306, 333, 522, 741, 837
SatI GCNGC 7 cut(s) 78, 621, 814, 1199, 1202, 1293, 1313
Sau3AI GATC 3 cut(s) 22, 172, 769
Sau96I GGNCC 4 cut(s) 129, 249, 484, 1261
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 3 cut(s) 74, 273, 295
ScrFI CCNGG 6 cut(s) 146, 406, 953, 1164, 1247, 1248
SduI GDGCHC 2 cut(s) 145, 153
SfaNI GCATC 6 cut(s) 353, 772, 876, 1047, 1114, 1168
SfcI CTRYAG 1 cut(s) 1221
SinI GGWCC 3 cut(s) 129, 249, 1261
SmaI CCCGGG 1 cut(s) 1248
SmiMI CAYNNNNRTG 2 cut(s) 138, 1164
Sse9I AATT 9 cut(s) 285, 307, 377, 451, 742, 831, 964, 1005, 1175
SsiI CCGC 6 cut(s) 78, 223, 514, 814, 1199, 1259
SspMI CTAG 3 cut(s) 26, 821, 1289
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 6 cut(s) 144, 404, 951, 1162, 1245, 1246
StyI CCWWGG 2 cut(s) 168, 245
TaaI ACNGT 2 cut(s) 431, 659
TaqI TCGA 1 cut(s) 772
TaqII GACCGA 1 cut(s) 931
TasI AATT 9 cut(s) 285, 307, 377, 451, 742, 831, 964, 1005, 1175
TatI WGTACW 2 cut(s) 659, 987
TauI GCSGC 3 cut(s) 80, 816, 1201
TfiI GAWTC 2 cut(s) 227, 409
Tru1I TTAA 5 cut(s) 306, 333, 522, 741, 837
Tru9I TTAA 5 cut(s) 306, 333, 522, 741, 837
TscAI CASTG 3 cut(s) 21, 910, 1088
TseI GCWGC 4 cut(s) 620, 1201, 1292, 1312
TspDTI ATGAA 3 cut(s) 224, 413, 740
TspGWI ACGGA 1 cut(s) 833
TspMI CCCGGG 1 cut(s) 1246
TspRI CASTG 3 cut(s) 21, 910, 1088
VpaK11BI GGWCC 3 cut(s) 129, 249, 1261
XapI RAATTY 1 cut(s) 1175
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 2 cut(s) 210, 1015
XmaI CCCGGG 1 cut(s) 1246
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 2 cut(s) 231, 714
XspI CTAG 3 cut(s) 26, 821, 1289
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.