RchiOBHm_Chr4g0406481

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006360
Physical Location & Seq
Reverse (-)
28284028 .. 28290141
6114 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37783

Sequence Viewer

Length: 1410 bp
ATGAAGTCACCGAATCACTCTTTACAGCAGAAGAAGCTGGAAACAGCAACCGAGAGGCAGACTGAGAGAGAGAGAGAGATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACACATAATCCTGCGCAAGTTAGGAGCTGAAGAGTCAGGGAGAGCCGCGTGCGTCAGCAAGCAACTCAGAGCTTCGGCCTCTGAAGAACCTCTTTGGTCCCATTTCTGTGCCCAAGAGCTCCACCTCTCACAACCCTTGGATCCTCATGGCAACCTTGCTCCTTCTTTCAAGGCATGTTATCAATCATGGCGGGAATCTTTTCATATGTATCCTTGGTCCCTTGTCAAGCGAGTCAAAAGGTGTTGGGGCAAAATTACCAACTGGCTGACTCTTAATTTCCCAGAGGCTCAGTCTACGCTTAACAAAGGTGCGTCAGAAGCTGACATTCAAGAGTTGGAGAACACATTGAAAGTAAAACTTCCCCTCCCAACCAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTAGAGGACAAGAATTGGTCAAGTAGCCGAAATGGCACTCTACTGGGCCTCATAGGTGGTTATTCTTTTTATGACCGCATGGTTAATGTCCGCTTGTTGCCTTTACATCAGATAGTATTAGCAACAAAGGACATCATGGAAACGCTTGATTTTTCAGCAACACCGGATTATATTGTTGTGGCTGCTTCTACATTTAGCCAAAAGTTGTTTTTCCTCAACTGTACAAGTGGTCAACTCTATGTTGGTACGGCCAACCTTCTGCTTGATGGTGAAATGCTTCCTTGTGTACCGAATGCGTTGGTTAATTCAGTTCATGATTACAACGGTGGTGATCGACAGCAGGATGCCATGCTTCTTTGGTTGGAAGAACACGGCCGCCGTCTAGAAAATGGAATTATTAAACTCCGTGAACAACAAACTATGAGAAGCATCTGTCAGTTTCCAGAGGAACCCCCTTTCTGTTCCACTGCCGTAACAAGTGGCGTACAGGTTCGTTGTTCTTCGGTCTTTGTCCCGGAGTTTACTAATCTTCAAGATACCATCAGGAAGTACTTTTTTTCCTATTCAATTCGCATGTCGCTTGTACCTGAAGGATGTAGCATCAATGGAATGACATTCAACTCTTGCCAACTGCTTTCAAGGCACTGGGTCATTCGTGCTGGTGATGTTGTGGTAGGTAATGTAGATGCAGAGGCTGTTATAGGAAAGTACCCTCTCTTGCATCCTGGTGAGCCAGAATTTGTATATGAGAGTTGCACGCCGCTGCCATCTTTGTCAGGTTCTATAGAAGGTTCTTTTGTGTTTGTCCCGGGCAGGTTGGCGGACCCAAAAGGCAGAGGATTTGAAGTTCTAGCTGCACGCTTTCCCCTCCAGCTGCCAGACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

469

Amino Acids

52.76

Weight (kDa)

6.09

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 351 - 441 1.5e-20 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 125
Acc36I ACCTGC 1 cut(s) 1320
AccI GTMKAC 1 cut(s) 404
AccII CGCG 1 cut(s) 158
AciI CCGC 7 cut(s) 156, 301, 592, 607, 892, 1277, 1337
AclWI GGATC 2 cut(s) 245, 258
AcoI YGGCCR 2 cut(s) 765, 889
AcsI RAATTY 1 cut(s) 1253
AcuI CTGAAG 3 cut(s) 159, 213, 1125
AfaI GTAC 7 cut(s) 739, 763, 804, 1002, 1067, 1101, 1226
AfeI AGCGCT 1 cut(s) 108
AgsI TTSAA 8 cut(s) 280, 440, 460, 1049, 1083, 1135, 1155, 1361
AjnI CCWGG 2 cut(s) 482, 1240
AloI GAACNNNNNNTCC 2 cut(s) 1347, 1379
AluBI AGCT 7 cut(s) 37, 137, 182, 229, 431, 1370, 1390
AluI AGCT 7 cut(s) 37, 137, 182, 229, 431, 1370, 1390
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 2 cut(s) 245, 258
AlwNI CAGNNNCTG 2 cut(s) 431, 1211
Ama87I CYCGRG 1 cut(s) 1324
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 4 cut(s) 186, 562, 765, 889
ApeKI GCWGC 4 cut(s) 698, 1279, 1370, 1390
ApoI RAATTY 1 cut(s) 1253
Asp700I GAANNNNTTC 2 cut(s) 309, 792
AspLEI GCGC 2 cut(s) 109, 126
AspS9I GGNCC 4 cut(s) 207, 327, 562, 1339
AsuC2I CCSGG 3 cut(s) 1031, 1325, 1326
AsuHPI GGTGA 5 cut(s) 110, 797, 857, 1190, 1256
AvaI CYCGRG 1 cut(s) 1324
AvaII GGWCC 3 cut(s) 207, 327, 1339
BaeGI GKGCMC 1 cut(s) 223
BamHI GGATCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 685, 1266, 1357, 1377
BccI CCATC 4 cut(s) 73, 776, 1064, 1291
BceAI ACGGC 4 cut(s) 780, 879, 904, 971
BciT130I CCWGG 2 cut(s) 484, 1242
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 3 cut(s) 1031, 1325, 1326
BfaI CTAG 3 cut(s) 104, 899, 1367
BfmI CTRYAG 1 cut(s) 1299
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 1320
BfuI GTATCC 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 549
BisI GCNGC 7 cut(s) 156, 699, 892, 1277, 1280, 1371, 1391
BlsI GCNGC 7 cut(s) 157, 700, 893, 1278, 1281, 1372, 1392
BmcAI AGTACT 1 cut(s) 1067
Bme1390I CCNGG 5 cut(s) 484, 1031, 1242, 1325, 1326
Bme18I GGWCC 3 cut(s) 207, 327, 1339
BmeT110I CYCGRG 1 cut(s) 1324
BmgT120I GGNCC 4 cut(s) 207, 327, 562, 1339
BmiI GGNNCC 5 cut(s) 209, 252, 329, 966, 1341
BmrFI CCNGG 5 cut(s) 484, 1031, 1242, 1325, 1326
BmrI ACTGGG 2 cut(s) 569, 1171
BmsI GCATC 5 cut(s) 850, 954, 1125, 1192, 1246
BmuI ACTGGG 2 cut(s) 569, 1171
BpmI CTGGAG 1 cut(s) 1370
BpuMI CCSGG 3 cut(s) 1031, 1325, 1326
BsaJI CCNNGG 3 cut(s) 246, 323, 1324
BsaWI WCCGGW 1 cut(s) 679
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bse1I ACTGG 3 cut(s) 377, 564, 1166
BseBI CCWGG 2 cut(s) 484, 1242
BseDI CCNNGG 3 cut(s) 246, 323, 1324
BseGI GGATG 3 cut(s) 865, 1115, 1237
BseMII CTCAG 3 cut(s) 54, 190, 413
BseNI ACTGG 3 cut(s) 377, 564, 1166
BseSI GKGCMC 1 cut(s) 223
BseX3I CGGCCG 1 cut(s) 889
BseXI GCAGC 4 cut(s) 685, 1266, 1357, 1377
BsgI GTGCAG 1 cut(s) 1356
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 892
BshFI GGCC 4 cut(s) 188, 564, 767, 891
BsiEI CGRYCG 1 cut(s) 892
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 1324
BsiSI CCGG 3 cut(s) 680, 1031, 1325
BslFI GGGAC 4 cut(s) 193, 313, 1013, 1307
BsmFI GGGAC 4 cut(s) 193, 313, 1013, 1307
BsmI GAATGC 1 cut(s) 814
BsnI GGCC 4 cut(s) 188, 564, 767, 891
BsoBI CYCGRG 1 cut(s) 1324
Bsp1286I GDGCHC 2 cut(s) 223, 231
Bsp1407I TGTACA 1 cut(s) 737
Bsp143I GATC 3 cut(s) 100, 250, 847
BspACI CCGC 7 cut(s) 156, 301, 592, 607, 892, 1277, 1337
BspANI GGCC 4 cut(s) 188, 564, 767, 891
BspCNI CTCAG 3 cut(s) 55, 189, 412
BspFNI CGCG 1 cut(s) 158
BspHI TCATGA 2 cut(s) 502, 829
BspLI GGNNCC 5 cut(s) 209, 252, 329, 966, 1341
BspMI ACCTGC 1 cut(s) 1320
BspPI GGATC 2 cut(s) 245, 258
BsrGI TGTACA 1 cut(s) 737
BsrI ACTGG 3 cut(s) 377, 564, 1166
BssECI CCNNGG 3 cut(s) 246, 323, 1324
BssMI GATC 3 cut(s) 100, 250, 847
BssT1I CCWWGG 2 cut(s) 246, 323
Bst2UI CCWGG 2 cut(s) 484, 1242
Bst4CI ACNGT 3 cut(s) 509, 737, 842
Bst6I CTCTTC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 737
BstC8I GCNNGC 4 cut(s) 160, 170, 1274, 1375
BstDEI CTNAG 3 cut(s) 63, 176, 399
BstF5I GGATG 3 cut(s) 865, 1115, 1237
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 2 cut(s) 109, 126
BstKTI GATC 3 cut(s) 103, 253, 850
BstMBI GATC 3 cut(s) 100, 250, 847
BstMCI CGRYCG 1 cut(s) 892
BstMWI GCNNNNNNNGC 4 cut(s) 34, 428, 549, 1156
BstNI CCWGG 2 cut(s) 484, 1242
BstNSI RCATGY 2 cut(s) 288, 1093
BstSCI CCNGG 5 cut(s) 482, 1029, 1240, 1323, 1324
BstSFI CTRYAG 1 cut(s) 1299
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 4 cut(s) 685, 1266, 1357, 1377
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BstZI CGGCCG 1 cut(s) 889
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 4 cut(s) 188, 564, 767, 891
BtsCI GGATG 3 cut(s) 865, 1115, 1237
BtsI GCAGTG 2 cut(s) 99, 981
BtsIMutI CAGTG 3 cut(s) 99, 981, 1159
BveI ACCTGC 1 cut(s) 1320
Cac8I GCNNGC 4 cut(s) 160, 170, 1274, 1375
CaiI CAGNNNCTG 2 cut(s) 431, 1211
CciI TCATGA 2 cut(s) 502, 829
CfoI GCGC 2 cut(s) 109, 126
Cfr13I GGNCC 4 cut(s) 207, 327, 562, 1339
Cfr9I CCCGGG 1 cut(s) 1324
CseI GACGC 2 cut(s) 151, 411
Csp6I GTAC 7 cut(s) 738, 762, 803, 1001, 1066, 1100, 1225
CviAII CATG 9 cut(s) 257, 285, 297, 503, 595, 652, 830, 865, 1090
CviQI GTAC 7 cut(s) 738, 762, 803, 1001, 1066, 1100, 1225
DdeI CTNAG 3 cut(s) 63, 176, 399
DpnI GATC 3 cut(s) 102, 252, 849
DpnII GATC 3 cut(s) 100, 250, 847
EaeI YGGCCR 2 cut(s) 765, 889
EagI CGGCCG 1 cut(s) 889
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 1 cut(s) 1352
Ecl136II GAGCTC 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 889
Eco130I CCWWGG 2 cut(s) 246, 323
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 3 cut(s) 207, 327, 1339
Eco47III AGCGCT 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 3 cut(s) 159, 213, 1125
Eco88I CYCGRG 1 cut(s) 1324
EcoICRI GAGCTC 1 cut(s) 229
EcoRII CCWGG 2 cut(s) 482, 1240
EcoT14I CCWWGG 2 cut(s) 246, 323
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 2 cut(s) 246, 323
FaeI CATG 9 cut(s) 260, 288, 300, 506, 598, 655, 833, 868, 1093
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 453, 485
FaqI GGGAC 4 cut(s) 193, 313, 1013, 1307
FatI CATG 9 cut(s) 256, 284, 296, 502, 594, 651, 829, 864, 1089
FauI CCCGC 1 cut(s) 294
FauNDI CATATG 1 cut(s) 315
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 7 cut(s) 156, 699, 892, 1277, 1280, 1371, 1391
FokI GGATG 3 cut(s) 872, 1122, 1224
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 7 cut(s) 156, 699, 892, 1277, 1280, 1371, 1391
FspBI CTAG 3 cut(s) 104, 899, 1367
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 2 cut(s) 108, 125
GluI GCNGC 7 cut(s) 156, 699, 892, 1277, 1280, 1371, 1391
GsuI CTGGAG 1 cut(s) 1370
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 4 cut(s) 188, 564, 767, 891
HapII CCGG 3 cut(s) 680, 1031, 1325
HgaI GACGC 2 cut(s) 151, 411
HhaI GCGC 2 cut(s) 109, 126
Hin1II CATG 9 cut(s) 260, 288, 300, 506, 598, 655, 833, 868, 1093
Hin6I GCGC 2 cut(s) 107, 124
HinP1I GCGC 2 cut(s) 107, 124
HincII GTYRAC 1 cut(s) 749
HindII GTYRAC 1 cut(s) 749
HinfI GANTC 6 cut(s) 13, 143, 305, 342, 379, 487
HpaII CCGG 3 cut(s) 680, 1031, 1325
HphI GGTGA 5 cut(s) 110, 797, 857, 1190, 1256
Hpy166II GTNNAC 5 cut(s) 405, 749, 803, 926, 1038
Hpy188I TCNGA 5 cut(s) 179, 193, 427, 627, 1409
Hpy188III TCNNGA 8 cut(s) 440, 503, 512, 830, 899, 959, 1049, 1060
Hpy8I GTNNAC 5 cut(s) 405, 749, 803, 926, 1038
HpyAV CCTTC 4 cut(s) 282, 782, 1100, 1298
HpyCH4III ACNGT 3 cut(s) 509, 737, 842
HpyCH4V TGCA 4 cut(s) 1205, 1237, 1272, 1373
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 428, 549, 1156
HpyF3I CTNAG 3 cut(s) 63, 176, 399
Hsp92II CATG 9 cut(s) 260, 288, 300, 506, 598, 655, 833, 868, 1093
HspAI GCGC 2 cut(s) 107, 124
Kzo9I GATC 3 cut(s) 100, 250, 847
LmnI GCTCC 3 cut(s) 134, 234, 274
Lsp1109I GCAGC 4 cut(s) 685, 1266, 1357, 1377
LweI GCATC 5 cut(s) 850, 954, 1125, 1192, 1246
MaeI CTAG 3 cut(s) 104, 899, 1367
MaeIII GTNAC 2 cut(s) 6, 988
MalI GATC 3 cut(s) 102, 252, 849
MboI GATC 3 cut(s) 100, 250, 847
MboII GAAGA 7 cut(s) 43, 152, 206, 893, 1008, 1037, 1396
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 223, 231
MluCI AATT 7 cut(s) 363, 385, 529, 820, 909, 1083, 1253
MlyI GAGTC 3 cut(s) 152, 351, 373
MmeI TCCRAC 2 cut(s) 426, 858
MroXI GAANNNNTTC 2 cut(s) 309, 792
MseI TTAA 5 cut(s) 384, 411, 600, 819, 915
MslI CAYNNNNRTG 2 cut(s) 216, 1242
MspA1I CMGCKG 2 cut(s) 1279, 1390
MspI CCGG 3 cut(s) 680, 1031, 1325
MspR9I CCNGG 5 cut(s) 484, 1031, 1242, 1325, 1326
Mva1269I GAATGC 1 cut(s) 814
MvaI CCWGG 2 cut(s) 484, 1242
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 4 cut(s) 34, 428, 549, 1156
NciI CCSGG 3 cut(s) 1031, 1325, 1326
NdeI CATATG 1 cut(s) 315
NdeII GATC 3 cut(s) 100, 250, 847
NlaIII CATG 9 cut(s) 260, 288, 300, 506, 598, 655, 833, 868, 1093
NlaIV GGNNCC 5 cut(s) 209, 252, 329, 966, 1341
NmuCI GTSAC 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 125
NspI RCATGY 2 cut(s) 288, 1093
PagI TCATGA 2 cut(s) 502, 829
PctI GAATGC 1 cut(s) 814
PdmI GAANNNNTTC 2 cut(s) 309, 792
PfeI GAWTC 3 cut(s) 13, 305, 487
PfoI TCCNGGA 1 cut(s) 1029
PkrI GCNGC 7 cut(s) 157, 700, 893, 1278, 1281, 1372, 1392
PleI GAGTC 3 cut(s) 151, 350, 373
PpsI GAGTC 3 cut(s) 151, 350, 373
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 482, 1240
PspGI CCWGG 2 cut(s) 482, 1240
PspN4I GGNNCC 5 cut(s) 209, 252, 329, 966, 1341
PspPI GGNCC 4 cut(s) 207, 327, 562, 1339
PstNI CAGNNNCTG 2 cut(s) 431, 1211
PsuI RGATCY 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 1390
RsaI GTAC 7 cut(s) 739, 763, 804, 1002, 1067, 1101, 1226
RsaNI GTAC 7 cut(s) 738, 762, 803, 1001, 1066, 1100, 1225
RseI CAYNNNNRTG 2 cut(s) 216, 1242
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 5 cut(s) 384, 411, 600, 819, 915
SatI GCNGC 7 cut(s) 156, 699, 892, 1277, 1280, 1371, 1391
Sau3AI GATC 3 cut(s) 100, 250, 847
Sau96I GGNCC 4 cut(s) 207, 327, 562, 1339
ScaI AGTACT 1 cut(s) 1067
SchI GAGTC 3 cut(s) 152, 351, 373
ScrFI CCNGG 5 cut(s) 484, 1031, 1242, 1325, 1326
SduI GDGCHC 2 cut(s) 223, 231
SfaNI GCATC 5 cut(s) 850, 954, 1125, 1192, 1246
SfcI CTRYAG 1 cut(s) 1299
SinI GGWCC 3 cut(s) 207, 327, 1339
SmaI CCCGGG 1 cut(s) 1326
SmiMI CAYNNNNRTG 2 cut(s) 216, 1242
Sse9I AATT 7 cut(s) 363, 385, 529, 820, 909, 1083, 1253
SsiI CCGC 7 cut(s) 156, 301, 592, 607, 892, 1277, 1337
SspMI CTAG 3 cut(s) 104, 899, 1367
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 5 cut(s) 482, 1029, 1240, 1323, 1324
StyI CCWWGG 2 cut(s) 246, 323
TaaI ACNGT 3 cut(s) 509, 737, 842
TaqI TCGA 1 cut(s) 850
TaqII GACCGA 1 cut(s) 1009
TasI AATT 7 cut(s) 363, 385, 529, 820, 909, 1083, 1253
TatI WGTACW 2 cut(s) 737, 1065
TauI GCSGC 3 cut(s) 158, 894, 1279
TfiI GAWTC 3 cut(s) 13, 305, 487
Tru1I TTAA 5 cut(s) 384, 411, 600, 819, 915
Tru9I TTAA 5 cut(s) 384, 411, 600, 819, 915
TscAI CASTG 3 cut(s) 99, 988, 1166
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 4 cut(s) 698, 1279, 1370, 1390
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 4 cut(s) 17, 302, 491, 818
TspGWI ACGGA 1 cut(s) 911
TspMI CCCGGG 1 cut(s) 1324
TspRI CASTG 3 cut(s) 99, 988, 1166
VpaK11BI GGWCC 3 cut(s) 207, 327, 1339
XapI RAATTY 1 cut(s) 1253
XbaI TCTAGA 1 cut(s) 898
XceI RCATGY 2 cut(s) 288, 1093
XmaI CCCGGG 1 cut(s) 1324
XmiI GTMKAC 1 cut(s) 404
XmnI GAANNNNTTC 2 cut(s) 309, 792
XspI CTAG 3 cut(s) 104, 899, 1367
ZrmI AGTACT 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.