Rroxscaffold_5G00351360

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006360
Physical Location & Seq
Reverse (-)
27563150 .. 27570816
7667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351360.1

Sequence Viewer

Length: 1329 bp
ATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACAGATAATCCTGCGCAAGTTAGGAGCTGAAGAGTCAGGGAGAGCCGCCTGCGTGAGCAAGCAACTCAGAGCTTCGGCCTCTGAAGAACCTCTTTGGTCCCATTTCTGTGCCCAGGAGCTCCACCTCTCACAACCCTTGGATCCTCATGGCAACCTTGCTCCTTCTTTCAAGGCATGTTATCGATCATGGCGGGAATCTTTTCATATGTATCCTTGGTCCCTTGTCAAGCGAGTCAAAAGGTGTTGGGGCAAAATTACCAACTGGCTGACTCTTAATTTTCCAGAGGCTCAGTATACGCTTAACAAAGGTGCATCGGAAGCTGACATTCAAGAGTTGGAGAACACATTGAAAGTAAAACTTCCTCTCCCAACCAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTAGAGGACAAGAATTGGTCAAGTAGCCGAAATGGCACTCTACTGGGCCTCATAGGTGGTTATTCTTTTTATGACCGCATGGTAAATGTCTGCTTGTTGCCTTTACATCAGATAGTATTAGCAACAAAGGGCATCATGGAAAAGCTTGATTTTTTAGCAACAGCAGATTATATTGTTGTGGCTGCTTCTACATATAACCAAAAGTTGTTTTTCCTCAACTGTACAAGTGGTCAACTCTATGTTGGTACTGCCAACCTTCTGGTTGATGGTGAAATGCTTCCTTGTGTACCGAATGCGTTGGTTAATTCAGTTCATGATGACAACGGTGATCGACAGCAGGATGCCATGCTTCTTTGGTTGGAAGAACACGGCCGCCGTCTAGAAAATGGAATTATTAAACTCCGTGAACAAGAAACTATGAGAAGCATCTGTCAGTTTCCAGAGGAACCCCCTTTCTGTTCCACTGCCGTAACAAGTGGCGTACAGGTTCGTTGTTCTTCGGTCTTTGTCCCGGAGTTTACTAATCTTCAAGATACCATCCAGAAGTACTTTTTTTCCTATTCAATTCGCATGTCGCTTTTACCTGAAGGATGTAGCATCAATGGAATGACATTCAACTCCTGCCAACTGCTTTCGAGGCACTGGGTCATTCGTGCTGGGGATGTTGTGGTAGGTAATGTAAATGCAGAGGCTGTTATAGGAAAGTACCCTCTCTTGCGTCCAGGTGAGCCAGAATTTGTATATGAGAGTTGCACGACGCAGCCATCTTTGTCAGGTTCTATAGAAGGTTCTTTTGTGTTTGTCCCAGGCAGGTTGGCGGACCCAAAAGGCGGAGGATTTAAAGTTCCAGCTGCACGCTTTCCCCTCCAGCTGCCAGACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

442

Amino Acids

49.46

Weight (kDa)

6.03

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 323 - 414 1.9e-20 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 1239
AccI GTMKAC 1 cut(s) 326
AciI CCGC 6 cut(s) 78, 223, 514, 811, 1256, 1269
AclWI GGATC 2 cut(s) 167, 180
AcoI YGGCCR 1 cut(s) 808
AcsI RAATTY 1 cut(s) 1172
AcuI CTGAAG 3 cut(s) 81, 135, 1044
AfaI GTAC 6 cut(s) 661, 685, 726, 921, 986, 1145
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 1268
AgsI TTSAA 6 cut(s) 202, 362, 382, 968, 1002, 1054
AjnI CCWGG 4 cut(s) 144, 404, 1159, 1243
AloI GAACNNNNNNTCC 2 cut(s) 1266, 1298
AluBI AGCT 7 cut(s) 59, 104, 151, 353, 583, 1289, 1309
AluI AGCT 7 cut(s) 59, 104, 151, 353, 583, 1289, 1309
Alw21I GWGCWC 1 cut(s) 153
AlwI GGATC 2 cut(s) 167, 180
AlwNI CAGNNNCTG 1 cut(s) 1130
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 3 cut(s) 108, 484, 808
ApeKI GCWGC 4 cut(s) 620, 1198, 1289, 1309
ApoI RAATTY 1 cut(s) 1172
Asp700I GAANNNNTTC 2 cut(s) 231, 714
AspLEI GCGC 2 cut(s) 31, 48
AspS9I GGNCC 4 cut(s) 129, 249, 484, 1258
AsuC2I CCSGG 1 cut(s) 950
AsuHPI GGTGA 4 cut(s) 32, 719, 776, 1175
AvaII GGWCC 3 cut(s) 129, 249, 1258
BaeGI GKGCMC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 4 cut(s) 607, 1210, 1276, 1296
BccI CCATC 3 cut(s) 698, 983, 1210
BceAI ACGGC 3 cut(s) 798, 823, 890
BciT130I CCWGG 4 cut(s) 146, 406, 1161, 1245
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 950
BfaI CTAG 2 cut(s) 26, 818
BfmI CTRYAG 2 cut(s) 32, 1218
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 1239
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 471
BisI GCNGC 6 cut(s) 78, 621, 811, 1199, 1290, 1310
BlsI GCNGC 6 cut(s) 79, 622, 812, 1200, 1291, 1311
BmcAI AGTACT 1 cut(s) 986
Bme1390I CCNGG 5 cut(s) 146, 406, 950, 1161, 1245
Bme18I GGWCC 3 cut(s) 129, 249, 1258
BmgT120I GGNCC 4 cut(s) 129, 249, 484, 1258
BmiI GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
BmrFI CCNGG 5 cut(s) 146, 406, 950, 1161, 1245
BmrI ACTGGG 2 cut(s) 491, 1090
BmsI GCATC 5 cut(s) 353, 579, 769, 873, 1044
BmuI ACTGGG 2 cut(s) 491, 1090
BpmI CTGGAG 1 cut(s) 1289
BpuMI CCSGG 1 cut(s) 950
Bsa29I ATCGAT 1 cut(s) 214
BsaJI CCNNGG 4 cut(s) 144, 168, 245, 1243
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 1268
Bse1I ACTGG 3 cut(s) 299, 486, 1085
BseBI CCWGG 4 cut(s) 146, 406, 1161, 1245
BseCI ATCGAT 1 cut(s) 214
BseDI CCNNGG 4 cut(s) 144, 168, 245, 1243
BseGI GGATG 4 cut(s) 784, 975, 1034, 1105
BseLI CCNNNNNNNGG 1 cut(s) 1268
BseMII CTCAG 2 cut(s) 112, 335
BseNI ACTGG 3 cut(s) 299, 486, 1085
BseSI GKGCMC 1 cut(s) 145
BseX3I CGGCCG 1 cut(s) 808
BseXI GCAGC 4 cut(s) 607, 1210, 1276, 1296
BseYI CCCAGC 1 cut(s) 1094
BsgI GTGCAG 1 cut(s) 1275
Bsh1285I CGRYCG 1 cut(s) 811
BshFI GGCC 3 cut(s) 110, 486, 810
BshVI ATCGAT 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 811
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 950
BslFI GGGAC 4 cut(s) 115, 235, 932, 1226
BslI CCNNNNNNNGG 1 cut(s) 1268
BsmFI GGGAC 4 cut(s) 115, 235, 932, 1226
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 3 cut(s) 110, 486, 810
Bsp1286I GDGCHC 2 cut(s) 145, 153
Bsp1407I TGTACA 1 cut(s) 659
Bsp143I GATC 4 cut(s) 22, 172, 215, 766
BspACI CCGC 6 cut(s) 78, 223, 514, 811, 1256, 1269
BspANI GGCC 3 cut(s) 110, 486, 810
BspCNI CTCAG 2 cut(s) 111, 334
BspDI ATCGAT 1 cut(s) 214
BspHI TCATGA 2 cut(s) 424, 751
BspLI GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
BspMI ACCTGC 1 cut(s) 1239
BspPI GGATC 2 cut(s) 167, 180
BsrGI TGTACA 1 cut(s) 659
BsrI ACTGG 3 cut(s) 299, 486, 1085
BssECI CCNNGG 4 cut(s) 144, 168, 245, 1243
BssMI GATC 4 cut(s) 22, 172, 215, 766
BssNAI GTATAC 1 cut(s) 327
BssT1I CCWWGG 2 cut(s) 168, 245
Bst1107I GTATAC 1 cut(s) 327
Bst2UI CCWGG 4 cut(s) 146, 406, 1161, 1245
Bst4CI ACNGT 3 cut(s) 431, 659, 764
Bst6I CTCTTC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 659
BstC8I GCNNGC 3 cut(s) 82, 92, 1294
BstDEI CTNAG 2 cut(s) 98, 321
BstF5I GGATG 4 cut(s) 784, 975, 1034, 1105
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 48
BstKTI GATC 4 cut(s) 25, 175, 218, 769
BstMBI GATC 4 cut(s) 22, 172, 215, 766
BstMCI CGRYCG 1 cut(s) 811
BstMWI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
BstNI CCWGG 4 cut(s) 146, 406, 1161, 1245
BstNSI RCATGY 2 cut(s) 210, 1012
BstSCI CCNGG 5 cut(s) 144, 404, 948, 1159, 1243
BstSFI CTRYAG 2 cut(s) 32, 1218
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 4 cut(s) 607, 1210, 1276, 1296
BstX2I RGATCY 1 cut(s) 172
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 1 cut(s) 172
BstZ17I GTATAC 1 cut(s) 327
BstZI CGGCCG 1 cut(s) 808
Bsu15I ATCGAT 1 cut(s) 214
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 110, 486, 810
BsuTUI ATCGAT 1 cut(s) 214
BtsCI GGATG 4 cut(s) 784, 975, 1034, 1105
BtsI GCAGTG 2 cut(s) 21, 900
BtsIMutI CAGTG 3 cut(s) 21, 900, 1078
BveI ACCTGC 1 cut(s) 1239
Cac8I GCNNGC 3 cut(s) 82, 92, 1294
CaiI CAGNNNCTG 1 cut(s) 1130
CciI TCATGA 2 cut(s) 424, 751
CfoI GCGC 2 cut(s) 31, 48
Cfr13I GGNCC 4 cut(s) 129, 249, 484, 1258
ClaI ATCGAT 1 cut(s) 214
CseI GACGC 2 cut(s) 1145, 1204
Csp6I GTAC 6 cut(s) 660, 684, 725, 920, 985, 1144
CviAII CATG 9 cut(s) 179, 207, 219, 425, 517, 574, 752, 784, 1009
CviQI GTAC 6 cut(s) 660, 684, 725, 920, 985, 1144
DdeI CTNAG 2 cut(s) 98, 321
DpnI GATC 4 cut(s) 24, 174, 217, 768
DpnII GATC 4 cut(s) 22, 172, 215, 766
DraI TTTAAA 1 cut(s) 1279
EaeI YGGCCR 1 cut(s) 808
EagI CGGCCG 1 cut(s) 808
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 2 cut(s) 1271, 1284
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 1 cut(s) 808
Eco130I CCWWGG 2 cut(s) 168, 245
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 3 cut(s) 129, 249, 1258
Eco47III AGCGCT 1 cut(s) 30
Eco52I CGGCCG 1 cut(s) 808
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 81, 135, 1044
EcoICRI GAGCTC 1 cut(s) 151
EcoRII CCWGG 4 cut(s) 144, 404, 1159, 1243
EcoT14I CCWWGG 2 cut(s) 168, 245
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 2 cut(s) 168, 245
FaeI CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 787, 1012
FalI AAGNNNNNCTT 4 cut(s) 108, 140, 375, 407
FaqI GGGAC 4 cut(s) 115, 235, 932, 1226
FatI CATG 9 cut(s) 178, 206, 218, 424, 516, 573, 751, 783, 1008
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 237
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 6 cut(s) 78, 621, 811, 1199, 1290, 1310
FokI GGATG 4 cut(s) 791, 962, 1041, 1112
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 6 cut(s) 78, 621, 811, 1199, 1290, 1310
FspBI CTAG 2 cut(s) 26, 818
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 30, 47
GluI GCNGC 6 cut(s) 78, 621, 811, 1199, 1290, 1310
GsaI CCCAGC 1 cut(s) 1098
GsuI CTGGAG 1 cut(s) 1289
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 3 cut(s) 110, 486, 810
HapII CCGG 1 cut(s) 950
HgaI GACGC 2 cut(s) 1145, 1204
HhaI GCGC 2 cut(s) 31, 48
Hin1II CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 787, 1012
Hin6I GCGC 2 cut(s) 29, 46
HinP1I GCGC 2 cut(s) 29, 46
HincII GTYRAC 1 cut(s) 671
HindII GTYRAC 1 cut(s) 671
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 5 cut(s) 65, 227, 264, 301, 409
HpaII CCGG 1 cut(s) 950
HphI GGTGA 4 cut(s) 32, 719, 776, 1175
Hpy166II GTNNAC 5 cut(s) 327, 671, 725, 845, 957
Hpy188I TCNGA 5 cut(s) 101, 115, 349, 549, 1328
Hpy188III TCNNGA 9 cut(s) 314, 362, 425, 434, 752, 818, 878, 968, 979
Hpy8I GTNNAC 5 cut(s) 327, 671, 725, 845, 957
Hpy99I CGWCG 1 cut(s) 1198
HpyAV CCTTC 4 cut(s) 204, 704, 1019, 1217
HpyCH4III ACNGT 3 cut(s) 431, 659, 764
HpyCH4V TGCA 4 cut(s) 344, 1124, 1191, 1292
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
HpyF3I CTNAG 2 cut(s) 98, 321
Hsp92II CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 787, 1012
HspAI GCGC 2 cut(s) 29, 46
Kzo9I GATC 4 cut(s) 22, 172, 215, 766
LmnI GCTCC 4 cut(s) 56, 148, 156, 196
Lsp1109I GCAGC 4 cut(s) 607, 1210, 1276, 1296
LweI GCATC 5 cut(s) 353, 579, 769, 873, 1044
MaeI CTAG 2 cut(s) 26, 818
MaeIII GTNAC 1 cut(s) 907
MalI GATC 4 cut(s) 24, 174, 217, 768
MboI GATC 4 cut(s) 22, 172, 215, 766
MboII GAAGA 6 cut(s) 74, 128, 812, 927, 956, 1315
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 145, 153
MluCI AATT 7 cut(s) 285, 307, 451, 742, 828, 1002, 1172
MlyI GAGTC 3 cut(s) 74, 273, 295
MmeI TCCRAC 2 cut(s) 348, 777
MroXI GAANNNNTTC 2 cut(s) 231, 714
MseI TTAA 5 cut(s) 306, 333, 741, 834, 1278
MslI CAYNNNNRTG 1 cut(s) 138
MspA1I CMGCKG 2 cut(s) 1289, 1309
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 5 cut(s) 146, 406, 950, 1161, 1245
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 4 cut(s) 146, 406, 1161, 1245
MwoI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
NciI CCSGG 1 cut(s) 950
NdeI CATATG 1 cut(s) 237
NdeII GATC 4 cut(s) 22, 172, 215, 766
NlaIII CATG 9 cut(s) 182, 210, 222, 428, 520, 577, 755, 787, 1012
NlaIV GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 2 cut(s) 210, 1012
PagI TCATGA 2 cut(s) 424, 751
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 2 cut(s) 231, 714
PfeI GAWTC 2 cut(s) 227, 409
PfoI TCCNGGA 1 cut(s) 948
PkrI GCNGC 6 cut(s) 79, 622, 812, 1200, 1291, 1311
PleI GAGTC 3 cut(s) 73, 272, 295
PpsI GAGTC 3 cut(s) 73, 272, 295
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 4 cut(s) 144, 404, 1159, 1243
PspFI CCCAGC 1 cut(s) 1094
PspGI CCWGG 4 cut(s) 144, 404, 1159, 1243
PspN4I GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
PspPI GGNCC 4 cut(s) 129, 249, 484, 1258
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 1 cut(s) 172
PvuII CAGCTG 2 cut(s) 1289, 1309
RsaI GTAC 6 cut(s) 661, 685, 726, 921, 986, 1145
RsaNI GTAC 6 cut(s) 660, 684, 725, 920, 985, 1144
RseI CAYNNNNRTG 1 cut(s) 138
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 5 cut(s) 306, 333, 741, 834, 1278
SatI GCNGC 6 cut(s) 78, 621, 811, 1199, 1290, 1310
Sau3AI GATC 4 cut(s) 22, 172, 215, 766
Sau96I GGNCC 4 cut(s) 129, 249, 484, 1258
ScaI AGTACT 1 cut(s) 986
SchI GAGTC 3 cut(s) 74, 273, 295
ScrFI CCNGG 5 cut(s) 146, 406, 950, 1161, 1245
SduI GDGCHC 2 cut(s) 145, 153
SfaNI GCATC 5 cut(s) 353, 579, 769, 873, 1044
SfcI CTRYAG 2 cut(s) 32, 1218
SinI GGWCC 3 cut(s) 129, 249, 1258
SmiMI CAYNNNNRTG 1 cut(s) 138
Sse9I AATT 7 cut(s) 285, 307, 451, 742, 828, 1002, 1172
SsiI CCGC 6 cut(s) 78, 223, 514, 811, 1256, 1269
SspMI CTAG 2 cut(s) 26, 818
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 5 cut(s) 144, 404, 948, 1159, 1243
StyI CCWWGG 2 cut(s) 168, 245
TaaI ACNGT 3 cut(s) 431, 659, 764
TaqI TCGA 3 cut(s) 214, 769, 1073
TaqII GACCGA 1 cut(s) 928
TasI AATT 7 cut(s) 285, 307, 451, 742, 828, 1002, 1172
TatI WGTACW 2 cut(s) 659, 984
TauI GCSGC 2 cut(s) 80, 813
TfiI GAWTC 2 cut(s) 227, 409
Tru1I TTAA 5 cut(s) 306, 333, 741, 834, 1278
Tru9I TTAA 5 cut(s) 306, 333, 741, 834, 1278
TscAI CASTG 3 cut(s) 21, 907, 1085
TseI GCWGC 4 cut(s) 620, 1198, 1289, 1309
TspDTI ATGAA 3 cut(s) 224, 413, 740
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 3 cut(s) 21, 907, 1085
VpaK11BI GGWCC 3 cut(s) 129, 249, 1258
XapI RAATTY 1 cut(s) 1172
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 2 cut(s) 210, 1012
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 2 cut(s) 231, 714
XspI CTAG 2 cut(s) 26, 818
ZrmI AGTACT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.