Rw4G011310

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006360
Physical Location & Seq
Reverse (-)
26824245 .. 26830012
5768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011310.1

Sequence Viewer

Length: 1329 bp
ATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACACATAATCCTGCGCAAGTTAGGAGCTGAAGAGTCAGGGAGAGCCGCCTGCGTCAGCAAGCAACTCAGAGCTTCGGCCTCTGAAGAACCTCTTTGGTCCCATTTCTGTGCCCAGGAGCTCCACCTCTCACAACCCTTGGATCCTCATGGCAACCTTGCTCCTTCTTTCAAGGCATGTTATCAATCATGGCGGGAATCTTTTCATATGTATCCTTGGTCCCTTGTCAAGCGAGTCAAAAGGTGTTGGGGCAAAATTACCAACTGGCTGACTCTTAATTTCCCTGAGGCTCAGTCTACGCTTAACAAAGGTGCATCAGAAGCTGACATTCAAGAGTTGGAGAACACATTGAAAGTAAAACTTCCCCTCCCAACCAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTAGAGGACAAGAATCGGTCAAGTAGCCGAAATGGCACTCTACTGGGCCTCATAGGTGGTTATTCTTTTTATGACCGCATGGTTAATGTCTGCTTGTTGCCTTTACATCAGATAGTATTAGCAACAAAGGACATCATGGAAACGCTTGATTTTTCAGCAACACCAGATTATATTGTTGTGGCTGCTTCTACATATAGCCAAAAGTTGTTTTTCCTCAACTGTACTAGTGGTCAACTCTATGTTGGTACGGCCAACCTTGTGCTTGATGGTGAAATGCTTCCTTGTGTACCGAATGCGTTGGTTAATTCAGTTCATGATTACAACGGTGATCGACAGCAGGATGCCATGCTTCTTTGGTTGGAAGAACATGGCCGCCGTCTAGAAAATGGAATTATTAAACTCCGTGAACAACAAACTATGAGAAGCATCTGTCAGTTTCCAGAGGAACCCCCTCTCTGTTCCACTGCCGTAACAAGTGGCGTACAGGTTCGTTGTTCTTCGGTCTTTGTCCCGGAGTTTACTAATCTTCAAGATACCATCCAGAAGTACTTTTTTTCCTATTCAATTCGCATGTCGCTTGTACCTGAAGGATGTAGCATCAATGGAATGACATTCAACTCTTGCCAACTGCTTTCGAGGCACTGGGTCATTCGTGCTGGTGATGTTGTGGTAGGTAGTGTAAATGCAGAGGCTGTTATAGGAAAGTACCCTCTCTTGCGTCCAGGTGAGCCAGAATTTGTATATGAGAGTTGCACGCAGCAGCCATCTTTGTCAGGTTCTATAGAAGGTTTTTTTGTGTTTGTCCCGGGCAGGTTGGCGGACCCAAAAGGTGGAGGATTTAAAGTTCCAGCTGCACGCTTTCCCCTCCAGCTGCCAGACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

442

Amino Acids

49.4

Weight (kDa)

6.09

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 323 - 414 1.5e-19 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 1239
AccB7I CCANNNNNTGG 1 cut(s) 1268
AccI GTMKAC 1 cut(s) 326
AciI CCGC 5 cut(s) 78, 223, 514, 811, 1256
AclWI GGATC 2 cut(s) 167, 180
AcoI YGGCCR 2 cut(s) 687, 808
AcsI RAATTY 1 cut(s) 1172
AcuI CTGAAG 3 cut(s) 81, 135, 1044
AfaI GTAC 7 cut(s) 661, 685, 726, 921, 986, 1020, 1145
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 1268
AgsI TTSAA 6 cut(s) 202, 362, 382, 968, 1002, 1054
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 3 cut(s) 144, 404, 1159
AloI GAACNNNNNNTCC 2 cut(s) 1266, 1298
AluBI AGCT 6 cut(s) 59, 104, 151, 353, 1289, 1309
AluI AGCT 6 cut(s) 59, 104, 151, 353, 1289, 1309
Alw21I GWGCWC 1 cut(s) 153
AlwI GGATC 2 cut(s) 167, 180
AlwNI CAGNNNCTG 2 cut(s) 353, 1130
Ama87I CYCGRG 1 cut(s) 1243
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 4 cut(s) 108, 484, 687, 808
ApeKI GCWGC 5 cut(s) 620, 1195, 1198, 1289, 1309
ApoI RAATTY 1 cut(s) 1172
Asp700I GAANNNNTTC 2 cut(s) 231, 714
AspLEI GCGC 2 cut(s) 31, 48
AspS9I GGNCC 4 cut(s) 129, 249, 484, 1258
AsuC2I CCSGG 3 cut(s) 950, 1244, 1245
AsuHPI GGTGA 5 cut(s) 32, 719, 776, 1109, 1175
AvaI CYCGRG 1 cut(s) 1243
AvaII GGWCC 3 cut(s) 129, 249, 1258
AxyI CCTNAGG 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 5 cut(s) 607, 1207, 1210, 1276, 1296
BccI CCATC 3 cut(s) 698, 983, 1210
BceAI ACGGC 3 cut(s) 702, 798, 890
BciT130I CCWGG 3 cut(s) 146, 406, 1161
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 3 cut(s) 950, 1244, 1245
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 3 cut(s) 26, 663, 818
BfmI CTRYAG 1 cut(s) 1218
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 1239
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 471
BisI GCNGC 7 cut(s) 78, 621, 811, 1196, 1199, 1290, 1310
BlsI GCNGC 7 cut(s) 79, 622, 812, 1197, 1200, 1291, 1311
BmcAI AGTACT 1 cut(s) 986
Bme1390I CCNGG 6 cut(s) 146, 406, 950, 1161, 1244, 1245
Bme18I GGWCC 3 cut(s) 129, 249, 1258
BmeT110I CYCGRG 1 cut(s) 1243
BmgT120I GGNCC 4 cut(s) 129, 249, 484, 1258
BmiI GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
BmrFI CCNGG 6 cut(s) 146, 406, 950, 1161, 1244, 1245
BmrI ACTGGG 2 cut(s) 491, 1090
BmsI GCATC 4 cut(s) 353, 769, 873, 1044
BmuI ACTGGG 2 cut(s) 491, 1090
BpmI CTGGAG 1 cut(s) 1289
BpuMI CCSGG 3 cut(s) 950, 1244, 1245
BsaJI CCNNGG 4 cut(s) 144, 168, 245, 1243
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 1268
Bse1I ACTGG 3 cut(s) 299, 486, 1085
Bse21I CCTNAGG 1 cut(s) 315
BseBI CCWGG 3 cut(s) 146, 406, 1161
BseDI CCNNGG 4 cut(s) 144, 168, 245, 1243
BseGI GGATG 3 cut(s) 784, 975, 1034
BseLI CCNNNNNNNGG 1 cut(s) 1268
BseMII CTCAG 3 cut(s) 112, 306, 335
BseNI ACTGG 3 cut(s) 299, 486, 1085
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 5 cut(s) 607, 1207, 1210, 1276, 1296
BsgI GTGCAG 1 cut(s) 1275
BshFI GGCC 4 cut(s) 110, 486, 689, 810
BsiHKAI GWGCWC 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 1243
BsiSI CCGG 2 cut(s) 950, 1244
BslFI GGGAC 4 cut(s) 115, 235, 932, 1226
BslI CCNNNNNNNGG 1 cut(s) 1268
BsmFI GGGAC 4 cut(s) 115, 235, 932, 1226
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 4 cut(s) 110, 486, 689, 810
BsoBI CYCGRG 1 cut(s) 1243
Bsp1286I GDGCHC 2 cut(s) 145, 153
Bsp143I GATC 3 cut(s) 22, 172, 766
BspACI CCGC 5 cut(s) 78, 223, 514, 811, 1256
BspANI GGCC 4 cut(s) 110, 486, 689, 810
BspCNI CTCAG 3 cut(s) 111, 307, 334
BspHI TCATGA 2 cut(s) 424, 751
BspLI GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
BspMI ACCTGC 1 cut(s) 1239
BspPI GGATC 2 cut(s) 167, 180
BsrI ACTGG 3 cut(s) 299, 486, 1085
BssECI CCNNGG 4 cut(s) 144, 168, 245, 1243
BssMI GATC 3 cut(s) 22, 172, 766
BssT1I CCWWGG 2 cut(s) 168, 245
Bst2UI CCWGG 3 cut(s) 146, 406, 1161
Bst4CI ACNGT 3 cut(s) 431, 659, 764
Bst6I CTCTTC 1 cut(s) 57
BstC8I GCNNGC 4 cut(s) 82, 92, 1193, 1294
BstDEI CTNAG 3 cut(s) 98, 315, 321
BstF5I GGATG 3 cut(s) 784, 975, 1034
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 48
BstKTI GATC 3 cut(s) 25, 175, 769
BstMBI GATC 3 cut(s) 22, 172, 766
BstMWI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
BstNI CCWGG 3 cut(s) 146, 406, 1161
BstNSI RCATGY 2 cut(s) 210, 1012
BstSCI CCNGG 6 cut(s) 144, 404, 948, 1159, 1242, 1243
BstSFI CTRYAG 1 cut(s) 1218
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 5 cut(s) 607, 1207, 1210, 1276, 1296
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 315
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 4 cut(s) 110, 486, 689, 810
BtsCI GGATG 3 cut(s) 784, 975, 1034
BtsI GCAGTG 2 cut(s) 21, 900
BtsIMutI CAGTG 3 cut(s) 21, 900, 1078
BveI ACCTGC 1 cut(s) 1239
Cac8I GCNNGC 4 cut(s) 82, 92, 1193, 1294
CaiI CAGNNNCTG 2 cut(s) 353, 1130
CciI TCATGA 2 cut(s) 424, 751
CfoI GCGC 2 cut(s) 31, 48
Cfr13I GGNCC 4 cut(s) 129, 249, 484, 1258
Cfr9I CCCGGG 1 cut(s) 1243
CseI GACGC 2 cut(s) 73, 1145
Csp6I GTAC 7 cut(s) 660, 684, 725, 920, 985, 1019, 1144
CviQI GTAC 7 cut(s) 660, 684, 725, 920, 985, 1019, 1144
DdeI CTNAG 3 cut(s) 98, 315, 321
DpnI GATC 3 cut(s) 24, 174, 768
DpnII GATC 3 cut(s) 22, 172, 766
DraI TTTAAA 1 cut(s) 1279
EaeI YGGCCR 2 cut(s) 687, 808
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 1271
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 168, 245
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 3 cut(s) 129, 249, 1258
Eco47III AGCGCT 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 81, 135, 1044
Eco81I CCTNAGG 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 1243
EcoICRI GAGCTC 1 cut(s) 151
EcoRII CCWGG 3 cut(s) 144, 404, 1159
EcoT14I CCWWGG 2 cut(s) 168, 245
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 2 cut(s) 168, 245
FalI AAGNNNNNCTT 4 cut(s) 108, 140, 375, 407
FaqI GGGAC 4 cut(s) 115, 235, 932, 1226
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 237
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 7 cut(s) 78, 621, 811, 1196, 1199, 1290, 1310
FokI GGATG 3 cut(s) 791, 962, 1041
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 7 cut(s) 78, 621, 811, 1196, 1199, 1290, 1310
FspBI CTAG 3 cut(s) 26, 663, 818
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 30, 47
GluI GCNGC 7 cut(s) 78, 621, 811, 1196, 1199, 1290, 1310
GsuI CTGGAG 1 cut(s) 1289
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 4 cut(s) 110, 486, 689, 810
HapII CCGG 2 cut(s) 950, 1244
HgaI GACGC 2 cut(s) 73, 1145
HhaI GCGC 2 cut(s) 31, 48
Hin6I GCGC 2 cut(s) 29, 46
HinP1I GCGC 2 cut(s) 29, 46
HincII GTYRAC 1 cut(s) 671
HindII GTYRAC 1 cut(s) 671
HinfI GANTC 6 cut(s) 65, 227, 264, 301, 409, 451
HpaII CCGG 2 cut(s) 950, 1244
HphI GGTGA 5 cut(s) 32, 719, 776, 1109, 1175
Hpy166II GTNNAC 5 cut(s) 327, 671, 725, 845, 957
Hpy188I TCNGA 5 cut(s) 101, 115, 349, 549, 1328
Hpy188III TCNNGA 8 cut(s) 362, 425, 434, 752, 818, 878, 968, 979
Hpy8I GTNNAC 5 cut(s) 327, 671, 725, 845, 957
HpyAV CCTTC 3 cut(s) 204, 1019, 1217
HpyCH4III ACNGT 3 cut(s) 431, 659, 764
HpyCH4V TGCA 4 cut(s) 344, 1124, 1191, 1292
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
HpyF3I CTNAG 3 cut(s) 98, 315, 321
HspAI GCGC 2 cut(s) 29, 46
Kzo9I GATC 3 cut(s) 22, 172, 766
LmnI GCTCC 4 cut(s) 56, 148, 156, 196
Lsp1109I GCAGC 5 cut(s) 607, 1207, 1210, 1276, 1296
LweI GCATC 4 cut(s) 353, 769, 873, 1044
MaeI CTAG 3 cut(s) 26, 663, 818
MaeIII GTNAC 1 cut(s) 907
MalI GATC 3 cut(s) 24, 174, 768
MboI GATC 3 cut(s) 22, 172, 766
MboII GAAGA 6 cut(s) 74, 128, 812, 927, 956, 1315
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 145, 153
MluCI AATT 6 cut(s) 285, 307, 742, 828, 1002, 1172
MlyI GAGTC 3 cut(s) 74, 273, 295
MmeI TCCRAC 2 cut(s) 348, 777
MroXI GAANNNNTTC 2 cut(s) 231, 714
MseI TTAA 6 cut(s) 306, 333, 522, 741, 834, 1278
MslI CAYNNNNRTG 1 cut(s) 138
MspA1I CMGCKG 2 cut(s) 1289, 1309
MspI CCGG 2 cut(s) 950, 1244
MspR9I CCNGG 6 cut(s) 146, 406, 950, 1161, 1244, 1245
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 3 cut(s) 146, 406, 1161
MwoI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
NciI CCSGG 3 cut(s) 950, 1244, 1245
NdeI CATATG 1 cut(s) 237
NdeII GATC 3 cut(s) 22, 172, 766
NlaIV GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 2 cut(s) 210, 1012
PagI TCATGA 2 cut(s) 424, 751
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 2 cut(s) 231, 714
PfeI GAWTC 3 cut(s) 227, 409, 451
PflMI CCANNNNNTGG 1 cut(s) 1268
PfoI TCCNGGA 1 cut(s) 948
PkrI GCNGC 7 cut(s) 79, 622, 812, 1197, 1200, 1291, 1311
PleI GAGTC 3 cut(s) 73, 272, 295
PpsI GAGTC 3 cut(s) 73, 272, 295
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 144, 404, 1159
PspGI CCWGG 3 cut(s) 144, 404, 1159
PspN4I GGNNCC 5 cut(s) 131, 174, 251, 885, 1260
PspPI GGNCC 4 cut(s) 129, 249, 484, 1258
PstNI CAGNNNCTG 2 cut(s) 353, 1130
PsuI RGATCY 1 cut(s) 172
PvuII CAGCTG 2 cut(s) 1289, 1309
RsaI GTAC 7 cut(s) 661, 685, 726, 921, 986, 1020, 1145
RsaNI GTAC 7 cut(s) 660, 684, 725, 920, 985, 1019, 1144
RseI CAYNNNNRTG 1 cut(s) 138
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 6 cut(s) 306, 333, 522, 741, 834, 1278
SatI GCNGC 7 cut(s) 78, 621, 811, 1196, 1199, 1290, 1310
Sau3AI GATC 3 cut(s) 22, 172, 766
Sau96I GGNCC 4 cut(s) 129, 249, 484, 1258
ScaI AGTACT 1 cut(s) 986
SchI GAGTC 3 cut(s) 74, 273, 295
ScrFI CCNGG 6 cut(s) 146, 406, 950, 1161, 1244, 1245
SduI GDGCHC 2 cut(s) 145, 153
SfaNI GCATC 4 cut(s) 353, 769, 873, 1044
SfcI CTRYAG 1 cut(s) 1218
SinI GGWCC 3 cut(s) 129, 249, 1258
SmaI CCCGGG 1 cut(s) 1245
SmiMI CAYNNNNRTG 1 cut(s) 138
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 6 cut(s) 285, 307, 742, 828, 1002, 1172
SsiI CCGC 5 cut(s) 78, 223, 514, 811, 1256
SspMI CTAG 3 cut(s) 26, 663, 818
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 6 cut(s) 144, 404, 948, 1159, 1242, 1243
StyI CCWWGG 2 cut(s) 168, 245
TaaI ACNGT 3 cut(s) 431, 659, 764
TaqI TCGA 2 cut(s) 769, 1073
TaqII GACCGA 2 cut(s) 444, 928
TasI AATT 6 cut(s) 285, 307, 742, 828, 1002, 1172
TatI WGTACW 2 cut(s) 659, 984
TauI GCSGC 2 cut(s) 80, 813
TfiI GAWTC 3 cut(s) 227, 409, 451
Tru1I TTAA 6 cut(s) 306, 333, 522, 741, 834, 1278
Tru9I TTAA 6 cut(s) 306, 333, 522, 741, 834, 1278
TscAI CASTG 3 cut(s) 21, 907, 1085
TseI GCWGC 5 cut(s) 620, 1195, 1198, 1289, 1309
TspDTI ATGAA 3 cut(s) 224, 413, 740
TspGWI ACGGA 1 cut(s) 830
TspMI CCCGGG 1 cut(s) 1243
TspRI CASTG 3 cut(s) 21, 907, 1085
Van91I CCANNNNNTGG 1 cut(s) 1268
VpaK11BI GGWCC 3 cut(s) 129, 249, 1258
XapI RAATTY 1 cut(s) 1172
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 2 cut(s) 210, 1012
XmaI CCCGGG 1 cut(s) 1243
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 2 cut(s) 231, 714
XspI CTAG 3 cut(s) 26, 663, 818
ZrmI AGTACT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.