Prupe.1G197800_v2.0.a1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0006360
Physical Location & Seq
Reverse (-)
18929568 .. 18937190
7623 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G197800.4

Sequence Viewer

Length: 1323 bp
ATGATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACACACAATCCTAAGCAAATTAGAAGCCGAAGACTCAGCAAGAGCAGCCTGCGTGAGCAAGAAACTCAGAGCTTCGGCCTCCGAAGACTCTCTTTGGTCCCATTTCTGTGCTCGAGACCTCGACCTTTCGCAACCCCTCGACCCTCATGGCAACCTCACCCCTTCTTTCAAGGAAGGTTACCAATTATGGCGTGAAGCTTTTCATATGTATCCTTGGTCGCTTGTAAAGCGAGTCAAAAAATGTTGGGACAAACTTAGAAACTGGTTGACTATTAATTTTCCAGAGGCTGAGTCTACACTGAACAAGGGTGCATCAGAAGATGATATTCAAGAGTTGGAGAAAATCTTGAAAGTGAAACTTCCTCTCCCAACGAGGATCCTCTATCGTTTTCATGATGGTCAAGATTTTGAGGACAAACATTTTCAAAATAGCCTGGTTGGCTGTCCATTGGGCATCATAGGTGGTTATTCATTTTATAACCACTTGGTAACTGTGTATTTGTTGCCTTTACGTCAGGTCATCTCAGAAACAAAGGAGATAACACCCAAACTGGATTTTCCTGGCAGATCGAAGTGTGTTGTTGTGGCTGCATCTTGTACATATAGTGAGAAGTTGTTTTTCCTGAACTGTACAAGTGGTCAACTCTATGTTGGTACCAGAAATCTTCTAGATGATGGGGAAATGCTTCCTTGTGTACCAAATGCGTTGATTAGTTCAGTTCATGATTGCAGTGTTGATCAACAGCAGGATGCAATGCTGCTTTGGTTAGAAGAACACGGCCGTCGTTTAGAAAATGGCATTATTAAACTTCGTCAAGAAGAAAATTTCAGAAGCATCAGTCAGTTTCCAGAAGAATCCCCTCTCTGTTCCACTGCTATAACAAATGGTGTAAAGGTTCGTGCTTCTGCTGTGTTTGTCCCGGAGCAGGCTACTTCTCAAAAGTATTCATTTGCTTATTCAATCCGCATGTCCCTTCTTCCTGAAGGATGCATCATCAATGGAATGACATTCAGCTCTTGCCAACTGCATTGGAGGCACTGGATCATCCGTGCTAAAGATGTCGTGATAGCTGATGTTAATGGAGAAGCTGTTGTAGGACAGTACCCACTTTTGCATCCAGGCGACAGCGAATTTGTTTATGAGAGTTGCACACTTCTACCATTTTCTTCTGGTTCTATTGAAGGTTCTTTTGCTTTTATCCCTGGCAGTTTGAGGGAGCCAAAGGGCGGCCCATTTGATGTTGCAGTGGCGCAGTTCCCCCTCCAGGTGCCCGACTACATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

441

Amino Acids

49.41

Weight (kDa)

5.6

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 513
Acc65I GGTACC 1 cut(s) 689
AccB1I GGYRCC 2 cut(s) 689, 1303
AccI GTMKAC 1 cut(s) 329
AciI CCGC 2 cut(s) 1000, 1263
AclWI GGATC 3 cut(s) 406, 419, 1085
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 2 cut(s) 859, 1166
AcuI CTGAAG 1 cut(s) 1038
AfaI GTAC 5 cut(s) 634, 667, 691, 732, 1139
AfeI AGCGCT 1 cut(s) 33
AfiI CCNNNNNNNGG 3 cut(s) 961, 1262, 1300
AgsI TTSAA 6 cut(s) 205, 365, 385, 461, 996, 1217
AjnI CCWGG 5 cut(s) 468, 595, 1153, 1237, 1299
AluBI AGCT 5 cut(s) 107, 233, 1050, 1106, 1124
AluI AGCT 5 cut(s) 107, 233, 1050, 1106, 1124
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 3 cut(s) 406, 419, 1085
AlwNI CAGNNNCTG 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 147
Aor51HI AGCGCT 1 cut(s) 33
AoxI GGCC 3 cut(s) 111, 814, 1264
ApeKI GCWGC 3 cut(s) 80, 623, 793
ApoI RAATTY 2 cut(s) 859, 1166
ArsI GACNNNNNNTTYG 2 cut(s) 1151, 1183
AseI ATTAAT 1 cut(s) 309
Asp700I GAANNNNTTC 2 cut(s) 234, 720
Asp718I GGTACC 1 cut(s) 689
AspLEI GCGC 2 cut(s) 34, 1288
AspS9I GGNCC 2 cut(s) 132, 1265
AsuC2I CCSGG 1 cut(s) 956
AsuHPI GGTGA 2 cut(s) 35, 184
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 1 cut(s) 132
BaeGI GKGCMC 1 cut(s) 1308
BamHI GGATCC 1 cut(s) 411
BanI GGYRCC 2 cut(s) 689, 1303
BbsI GAAGAC 2 cut(s) 72, 126
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 92, 610, 780
BccI CCATC 2 cut(s) 425, 704
BceAI ACGGC 2 cut(s) 801, 829
BciT130I CCWGG 5 cut(s) 470, 597, 1155, 1239, 1301
BciVI GTATCC 1 cut(s) 255
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 1 cut(s) 956
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 2 cut(s) 29, 704
BfoI RGCGCY 1 cut(s) 35
BfuI GTATCC 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 474
BisI GCNGC 4 cut(s) 81, 624, 794, 1264
BlsI GCNGC 4 cut(s) 82, 625, 795, 1265
Bme1390I CCNGG 6 cut(s) 470, 597, 956, 1155, 1239, 1301
Bme18I GGWCC 1 cut(s) 132
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 2 cut(s) 132, 1265
BmiI GGNNCC 5 cut(s) 134, 413, 691, 1254, 1305
BmrFI CCNGG 6 cut(s) 470, 597, 956, 1155, 1239, 1301
BmsI GCATC 8 cut(s) 356, 498, 635, 775, 879, 1013, 1035, 1159
BpiI GAAGAC 2 cut(s) 72, 126
BpmI CTGGAG 1 cut(s) 1283
Bpu10I CCTNAGC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 956
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 248, 1237
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 1110, 1140
Bsc4I CCNNNNNNNGG 3 cut(s) 961, 1262, 1300
Bse1I ACTGG 3 cut(s) 302, 591, 1079
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 5 cut(s) 470, 597, 1155, 1239, 1301
BseDI CCNNGG 2 cut(s) 248, 1237
BseGI GGATG 4 cut(s) 790, 1028, 1080, 1150
BseLI CCNNNNNNNGG 3 cut(s) 961, 1262, 1300
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 4 cut(s) 84, 115, 315, 573
BseNI ACTGG 3 cut(s) 302, 591, 1079
BseSI GKGCMC 1 cut(s) 1308
BseX3I CGGCCG 1 cut(s) 814
BseXI GCAGC 3 cut(s) 92, 610, 780
Bsh1285I CGRYCG 1 cut(s) 817
BshFI GGCC 3 cut(s) 113, 816, 1266
BshNI GGYRCC 2 cut(s) 689, 1303
BsiEI CGRYCG 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 956
BslFI GGGAC 4 cut(s) 118, 296, 938, 991
BslI CCNNNNNNNGG 3 cut(s) 961, 1262, 1300
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 4 cut(s) 118, 296, 938, 991
BsnI GGCC 3 cut(s) 113, 816, 1266
Bso31I GGTCTC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 148, 1308
Bsp1407I TGTACA 2 cut(s) 632, 665
Bsp143I GATC 5 cut(s) 25, 411, 602, 772, 1077
BspACI CCGC 2 cut(s) 1000, 1263
BspANI GGCC 3 cut(s) 113, 816, 1266
BspCNI CTCAG 4 cut(s) 83, 114, 316, 572
BspHI TCATGA 2 cut(s) 427, 757
BspLI GGNNCC 5 cut(s) 134, 413, 691, 1254, 1305
BspPI GGATC 3 cut(s) 406, 419, 1085
BspT107I GGYRCC 2 cut(s) 689, 1303
BspTNI GGTCTC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 795
BsrGI TGTACA 2 cut(s) 632, 665
BsrI ACTGG 3 cut(s) 302, 591, 1079
BssECI CCNNGG 2 cut(s) 248, 1237
BssMI GATC 5 cut(s) 25, 411, 602, 772, 1077
BssT1I CCWWGG 1 cut(s) 248
Bst2UI CCWGG 5 cut(s) 470, 597, 1155, 1239, 1301
Bst4CI ACNGT 3 cut(s) 529, 665, 1137
BstAUI TGTACA 2 cut(s) 632, 665
BstC8I GCNNGC 2 cut(s) 85, 963
BstDEI CTNAG 6 cut(s) 47, 70, 101, 290, 324, 559
BstEII GGTNACC 1 cut(s) 212
BstF5I GGATG 4 cut(s) 790, 1028, 1080, 1150
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 2 cut(s) 34, 1288
BstKTI GATC 5 cut(s) 28, 414, 605, 775, 1080
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 5 cut(s) 25, 411, 602, 772, 1077
BstMCI CGRYCG 1 cut(s) 817
BstMWI GCNNNNNNNGC 4 cut(s) 80, 262, 474, 1069
BstNI CCWGG 5 cut(s) 470, 597, 1155, 1239, 1301
BstNSI RCATGY 1 cut(s) 1006
BstPI GGTNACC 1 cut(s) 212
BstSCI CCNGG 6 cut(s) 468, 595, 954, 1153, 1237, 1299
BstSLI GKGCMC 1 cut(s) 1308
BstV1I GCAGC 3 cut(s) 92, 610, 780
BstV2I GAAGAC 2 cut(s) 72, 126
BstX2I RGATCY 1 cut(s) 411
BstYI RGATCY 1 cut(s) 411
BstZI CGGCCG 1 cut(s) 814
BsuI GTATCC 1 cut(s) 255
BsuRI GGCC 3 cut(s) 113, 816, 1266
BtsCI GGATG 4 cut(s) 790, 1028, 1080, 1150
BtsI GCAGTG 4 cut(s) 24, 772, 906, 1287
BtsIMutI CAGTG 6 cut(s) 24, 332, 772, 906, 1072, 1287
Cac8I GCNNGC 2 cut(s) 85, 963
CaiI CAGNNNCTG 1 cut(s) 323
CciI TCATGA 2 cut(s) 427, 757
CfoI GCGC 2 cut(s) 34, 1288
Cfr13I GGNCC 2 cut(s) 132, 1265
Csp6I GTAC 5 cut(s) 633, 666, 690, 731, 1138
CviAII CATG 4 cut(s) 182, 428, 758, 1003
CviQI GTAC 5 cut(s) 633, 666, 690, 731, 1138
DdeI CTNAG 6 cut(s) 47, 70, 101, 290, 324, 559
DpnI GATC 5 cut(s) 27, 413, 604, 774, 1079
DpnII GATC 5 cut(s) 25, 411, 602, 772, 1077
EaeI YGGCCR 1 cut(s) 814
EagI CGGCCG 1 cut(s) 814
EclXI CGGCCG 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 248
Eco31I GGTCTC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 132
Eco47III AGCGCT 1 cut(s) 33
Eco52I CGGCCG 1 cut(s) 814
Eco57I CTGAAG 1 cut(s) 1038
Eco88I CYCGRG 1 cut(s) 147
Eco91I GGTNACC 1 cut(s) 212
EcoO65I GGTNACC 1 cut(s) 212
EcoRII CCWGG 5 cut(s) 468, 595, 1153, 1237, 1299
EcoT14I CCWWGG 1 cut(s) 248
EcoT22I ATGCAT 1 cut(s) 1028
ErhI CCWWGG 1 cut(s) 248
FaeI CATG 4 cut(s) 185, 431, 761, 1006
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 378, 410
FaqI GGGAC 4 cut(s) 118, 296, 938, 991
FatI CATG 4 cut(s) 181, 427, 757, 1002
FauNDI CATATG 1 cut(s) 240
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 4 cut(s) 81, 624, 794, 1264
FokI GGATG 4 cut(s) 797, 1035, 1067, 1137
Fsp4HI GCNGC 4 cut(s) 81, 624, 794, 1264
FspBI CTAG 2 cut(s) 29, 704
GlaI GCGC 2 cut(s) 33, 1287
GluI GCNGC 4 cut(s) 81, 624, 794, 1264
GsuI CTGGAG 1 cut(s) 1283
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 3 cut(s) 113, 816, 1266
HapII CCGG 1 cut(s) 956
HhaI GCGC 2 cut(s) 34, 1288
Hin1II CATG 4 cut(s) 185, 431, 761, 1006
Hin6I GCGC 2 cut(s) 32, 1286
HinP1I GCGC 2 cut(s) 32, 1286
HincII GTYRAC 2 cut(s) 303, 677
HindII GTYRAC 2 cut(s) 303, 677
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 5 cut(s) 68, 122, 267, 326, 890
HpaII CCGG 1 cut(s) 956
HphI GGTGA 2 cut(s) 35, 184
Hpy166II GTNNAC 4 cut(s) 303, 330, 677, 731
Hpy188I TCNGA 6 cut(s) 104, 118, 352, 562, 866, 1322
Hpy8I GTNNAC 4 cut(s) 303, 330, 677, 731
Hpy99I CGWCG 1 cut(s) 822
HpyAV CCTTC 5 cut(s) 203, 207, 1013, 1019, 1211
HpyCH4III ACNGT 3 cut(s) 529, 665, 1137
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 9 cut(s) 347, 626, 765, 788, 1026, 1063, 1150, 1185, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 262, 474, 1069
HpyF3I CTNAG 6 cut(s) 47, 70, 101, 290, 324, 559
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 4 cut(s) 185, 431, 761, 1006
HspAI GCGC 2 cut(s) 32, 1286
KpnI GGTACC 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 5 cut(s) 25, 411, 602, 772, 1077
LmnI GCTCC 2 cut(s) 958, 1252
Lsp1109I GCAGC 3 cut(s) 92, 610, 780
LweI GCATC 8 cut(s) 356, 498, 635, 775, 879, 1013, 1035, 1159
MaeI CTAG 2 cut(s) 29, 704
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 2 cut(s) 212, 523
MalI GATC 5 cut(s) 27, 413, 604, 774, 1079
MboI GATC 5 cut(s) 25, 411, 602, 772, 1077
MflI RGATCY 1 cut(s) 411
MhlI GDGCHC 2 cut(s) 148, 1308
MluCI AATT 5 cut(s) 53, 218, 310, 859, 1166
MlyI GAGTC 4 cut(s) 62, 116, 276, 335
MmeI TCCRAC 1 cut(s) 351
Mph1103I ATGCAT 1 cut(s) 1028
MroXI GAANNNNTTC 2 cut(s) 234, 720
MseI TTAA 3 cut(s) 309, 840, 1113
MslI CAYNNNNRTG 1 cut(s) 141
MspI CCGG 1 cut(s) 956
MspR9I CCNGG 6 cut(s) 470, 597, 956, 1155, 1239, 1301
MvaI CCWGG 5 cut(s) 470, 597, 1155, 1239, 1301
MwoI GCNNNNNNNGC 4 cut(s) 80, 262, 474, 1069
NciI CCSGG 1 cut(s) 956
NdeI CATATG 1 cut(s) 240
NdeII GATC 5 cut(s) 25, 411, 602, 772, 1077
NlaIII CATG 4 cut(s) 185, 431, 761, 1006
NlaIV GGNNCC 5 cut(s) 134, 413, 691, 1254, 1305
NsiI ATGCAT 1 cut(s) 1028
NspI RCATGY 1 cut(s) 1006
PaeR7I CTCGAG 1 cut(s) 147
PagI TCATGA 2 cut(s) 427, 757
PdmI GAANNNNTTC 2 cut(s) 234, 720
PfeI GAWTC 1 cut(s) 890
PfoI TCCNGGA 1 cut(s) 954
PkrI GCNGC 4 cut(s) 82, 625, 795, 1265
PleI GAGTC 4 cut(s) 62, 116, 275, 334
PpsI GAGTC 4 cut(s) 62, 116, 275, 334
PshBI ATTAAT 1 cut(s) 309
PsiI TTATAA 1 cut(s) 513
Psp6I CCWGG 5 cut(s) 468, 595, 1153, 1237, 1299
PspEI GGTNACC 1 cut(s) 212
PspGI CCWGG 5 cut(s) 468, 595, 1153, 1237, 1299
PspN4I GGNNCC 5 cut(s) 134, 413, 691, 1254, 1305
PspPI GGNCC 2 cut(s) 132, 1265
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 411
RsaI GTAC 5 cut(s) 634, 667, 691, 732, 1139
RsaNI GTAC 5 cut(s) 633, 666, 690, 731, 1138
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 3 cut(s) 309, 840, 1113
SatI GCNGC 4 cut(s) 81, 624, 794, 1264
Sau3AI GATC 5 cut(s) 25, 411, 602, 772, 1077
Sau96I GGNCC 2 cut(s) 132, 1265
SchI GAGTC 4 cut(s) 62, 116, 276, 335
ScrFI CCNGG 6 cut(s) 470, 597, 956, 1155, 1239, 1301
SduI GDGCHC 2 cut(s) 148, 1308
SfaNI GCATC 8 cut(s) 356, 498, 635, 775, 879, 1013, 1035, 1159
Sfr274I CTCGAG 1 cut(s) 147
SinI GGWCC 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 147
SmoI CTYRAG 1 cut(s) 147
Sse9I AATT 5 cut(s) 53, 218, 310, 859, 1166
SsiI CCGC 2 cut(s) 1000, 1263
SspMI CTAG 2 cut(s) 29, 704
StyD4I CCNGG 6 cut(s) 468, 595, 954, 1153, 1237, 1299
StyI CCWWGG 1 cut(s) 248
TaaI ACNGT 3 cut(s) 529, 665, 1137
TaiI ACGT 1 cut(s) 550
TaqI TCGA 4 cut(s) 148, 156, 174, 605
TasI AATT 5 cut(s) 53, 218, 310, 859, 1166
TatI WGTACW 2 cut(s) 632, 665
TauI GCSGC 1 cut(s) 1266
TfiI GAWTC 1 cut(s) 890
Tru1I TTAA 3 cut(s) 309, 840, 1113
Tru9I TTAA 3 cut(s) 309, 840, 1113
TscAI CASTG 6 cut(s) 24, 339, 772, 913, 1079, 1287
TseI GCWGC 3 cut(s) 80, 623, 793
TspDTI ATGAA 5 cut(s) 227, 416, 495, 746, 972
TspGWI ACGGA 1 cut(s) 1073
TspRI CASTG 6 cut(s) 24, 339, 772, 913, 1079, 1287
VpaK11BI GGWCC 1 cut(s) 132
VspI ATTAAT 1 cut(s) 309
XapI RAATTY 2 cut(s) 859, 1166
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 1 cut(s) 1006
XhoI CTCGAG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 329
XmnI GAANNNNTTC 2 cut(s) 234, 720
XspI CTAG 2 cut(s) 29, 704
Zsp2I ATGCAT 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.