Rh4DG133200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006360
Physical Location & Seq
Reverse (-)
26558935 .. 26564967
6033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG133200.1

Sequence Viewer

Length: 1257 bp
ATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACACATAATCCTGCGCAAGTTAGGAGCTGAAGAGTCAGGGAGAGCCGCCTGCGTCAGCAAGCAACTCAGAGCTTCGGCCTCTGAAGAACCTCTTTGGTCCCATTTCTGTGCCCAGGAGCTCCACCTCTCACAACCCTTGGATCCTCATGGCAACCTTGCTCCTTCTTTCAAGGCATGTTATCAATCATGGCGGGAATCTTTTCATATGTATCCTTGGTCCCTTGTCAAGCGAGTCAAAAGGTGTTGGGGCAAAATTACCAACTGGCTGACTCTTAATTTCCCTGAGGCTCAGTCTACGCTTAACAAAGGTGCATCAGAAGCTGACATTCAAGAGTTGGAGAACACATTGAAAGTAAAACTTCCCCTCCCAACCAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTAGAGGACAAGAATCGGTCAAGTAGCCGAAATGGCACTCTACTGGGCCTCATAGGTGGTTATTCTTTTTATGACCGCATGGTTAATGTCTGCTTGTTGCCTTTACATCAGATAGTATTAGCAACAAAGGACATCATGGAAACGCTTGATTTTTCAGCAACACCAGATTATATTGTTGTGGCTGCTTCTACATATAGCCAAAAGTTGTTTTTCCTCAACTGTACTAGTGGTCAACTCTATGTTGGTACGGCCAACCTTGTGCTTGATGGTGAAATGCTTCCTTGTGTACCGAATGCGTTGGTTAATTCAGTTCATGATTACAACGGTGATCGACAGCAGGATGCCATGCTTCTTTGGTTGGAAGAACATGGCCGCCGTCTAGAAAATGGAATTATTAAACTCCGTGAACAACAAACTATGAGAAGCATCTGTCAGTTTCCAGAGGAACCCCCTCTCTGTTCCACTGCCGTAACAAGTGGCGTACAGGTTCGTTGTTCTTCGGTCTTTGTCCCGGAGTTTACTAATCTTCAAGATACCATCCAGAAGTACTTTTTTTCCTATTCAATTCGCATGTCGCTTGTACCTGAAGGATGTAGCATCAATGGAATGACATTCAACTCTTGCCAACTGCTTTCGAGGCACTGGGTCATTCGTGCTGGTGATGTTGTGGTAGGTAGTGTAAATGCAGAGGCTGTTATAGGAAAGGTACGATTTCACCTTGTCAATTACATTTGCTGTCTAATTCTTCCAAAGTCTTTTAGATCTGACAATTCTACACCTTTTCCTTTAGTGGACATGAGAATTATCATGATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

418

Amino Acids

47.07

Weight (kDa)

6.6

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 323 - 381 4.9e-08 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 47
AccI GTMKAC 1 cut(s) 326
AciI CCGC 4 cut(s) 78, 223, 514, 811
AclWI GGATC 2 cut(s) 167, 180
AcoI YGGCCR 2 cut(s) 687, 808
AcuI CTGAAG 3 cut(s) 81, 135, 1044
AfaI GTAC 7 cut(s) 661, 685, 726, 921, 986, 1020, 1146
AfeI AGCGCT 1 cut(s) 30
AgsI TTSAA 6 cut(s) 202, 362, 382, 968, 1002, 1054
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 2 cut(s) 144, 404
AluBI AGCT 4 cut(s) 59, 104, 151, 353
AluI AGCT 4 cut(s) 59, 104, 151, 353
Alw21I GWGCWC 1 cut(s) 153
AlwI GGATC 2 cut(s) 167, 180
AlwNI CAGNNNCTG 2 cut(s) 353, 1130
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 4 cut(s) 108, 484, 687, 808
ApeKI GCWGC 1 cut(s) 620
Asp700I GAANNNNTTC 2 cut(s) 231, 714
AspLEI GCGC 2 cut(s) 31, 48
AspS9I GGNCC 3 cut(s) 129, 249, 484
AsuC2I CCSGG 1 cut(s) 950
AsuHPI GGTGA 5 cut(s) 32, 719, 776, 1109, 1145
AvaII GGWCC 2 cut(s) 129, 249
AxyI CCTNAGG 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 1 cut(s) 607
BccI CCATC 2 cut(s) 698, 983
BceAI ACGGC 3 cut(s) 702, 798, 890
BciT130I CCWGG 2 cut(s) 146, 406
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 950
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 3 cut(s) 26, 663, 818
BfoI RGCGCY 1 cut(s) 32
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 471
BglII AGATCT 1 cut(s) 1199
BisI GCNGC 3 cut(s) 78, 621, 811
BlsI GCNGC 3 cut(s) 79, 622, 812
BmcAI AGTACT 1 cut(s) 986
Bme1390I CCNGG 3 cut(s) 146, 406, 950
Bme18I GGWCC 2 cut(s) 129, 249
BmgT120I GGNCC 3 cut(s) 129, 249, 484
BmiI GGNNCC 4 cut(s) 131, 174, 251, 885
BmrFI CCNGG 3 cut(s) 146, 406, 950
BmrI ACTGGG 2 cut(s) 491, 1090
BmsI GCATC 4 cut(s) 353, 769, 873, 1044
BmuI ACTGGG 2 cut(s) 491, 1090
BpuMI CCSGG 1 cut(s) 950
BsaJI CCNNGG 3 cut(s) 144, 168, 245
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bse1I ACTGG 3 cut(s) 299, 486, 1085
Bse21I CCTNAGG 1 cut(s) 315
BseBI CCWGG 2 cut(s) 146, 406
BseDI CCNNGG 3 cut(s) 144, 168, 245
BseGI GGATG 3 cut(s) 784, 975, 1034
BseMII CTCAG 3 cut(s) 112, 306, 335
BseNI ACTGG 3 cut(s) 299, 486, 1085
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 1 cut(s) 607
BshFI GGCC 4 cut(s) 110, 486, 689, 810
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 950
BslFI GGGAC 3 cut(s) 115, 235, 932
BsmFI GGGAC 3 cut(s) 115, 235, 932
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 4 cut(s) 110, 486, 689, 810
Bsp1286I GDGCHC 2 cut(s) 145, 153
Bsp143I GATC 5 cut(s) 22, 172, 766, 1199, 1248
BspACI CCGC 4 cut(s) 78, 223, 514, 811
BspANI GGCC 4 cut(s) 110, 486, 689, 810
BspCNI CTCAG 3 cut(s) 111, 307, 334
BspHI TCATGA 3 cut(s) 424, 751, 1245
BspLI GGNNCC 4 cut(s) 131, 174, 251, 885
BspPI GGATC 2 cut(s) 167, 180
BsrI ACTGG 3 cut(s) 299, 486, 1085
BssECI CCNNGG 3 cut(s) 144, 168, 245
BssMI GATC 5 cut(s) 22, 172, 766, 1199, 1248
BssT1I CCWWGG 2 cut(s) 168, 245
Bst2UI CCWGG 2 cut(s) 146, 406
Bst4CI ACNGT 3 cut(s) 431, 659, 764
Bst6I CTCTTC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 82, 92
BstDEI CTNAG 3 cut(s) 98, 315, 321
BstF5I GGATG 3 cut(s) 784, 975, 1034
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 48
BstKTI GATC 5 cut(s) 25, 175, 769, 1202, 1251
BstMBI GATC 5 cut(s) 22, 172, 766, 1199, 1248
BstMWI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
BstNI CCWGG 2 cut(s) 146, 406
BstNSI RCATGY 2 cut(s) 210, 1012
BstSCI CCNGG 3 cut(s) 144, 404, 948
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 607
BstX2I RGATCY 2 cut(s) 172, 1199
BstYI RGATCY 2 cut(s) 172, 1199
Bsu36I CCTNAGG 1 cut(s) 315
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 4 cut(s) 110, 486, 689, 810
BtsCI GGATG 3 cut(s) 784, 975, 1034
BtsI GCAGTG 2 cut(s) 21, 900
BtsIMutI CAGTG 3 cut(s) 21, 900, 1078
Cac8I GCNNGC 2 cut(s) 82, 92
CaiI CAGNNNCTG 2 cut(s) 353, 1130
CciI TCATGA 3 cut(s) 424, 751, 1245
CfoI GCGC 2 cut(s) 31, 48
Cfr13I GGNCC 3 cut(s) 129, 249, 484
CseI GACGC 1 cut(s) 73
Csp6I GTAC 7 cut(s) 660, 684, 725, 920, 985, 1019, 1145
CviQI GTAC 7 cut(s) 660, 684, 725, 920, 985, 1019, 1145
DdeI CTNAG 3 cut(s) 98, 315, 321
DpnI GATC 5 cut(s) 24, 174, 768, 1201, 1250
DpnII GATC 5 cut(s) 22, 172, 766, 1199, 1248
EaeI YGGCCR 2 cut(s) 687, 808
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 168, 245
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 2 cut(s) 129, 249
Eco47III AGCGCT 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 81, 135, 1044
Eco81I CCTNAGG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 144, 404
EcoT14I CCWWGG 2 cut(s) 168, 245
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 2 cut(s) 168, 245
FalI AAGNNNNNCTT 4 cut(s) 108, 140, 375, 407
FaqI GGGAC 3 cut(s) 115, 235, 932
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 237
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 78, 621, 811
FokI GGATG 3 cut(s) 791, 962, 1041
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 3 cut(s) 78, 621, 811
FspBI CTAG 3 cut(s) 26, 663, 818
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 30, 47
GluI GCNGC 3 cut(s) 78, 621, 811
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 4 cut(s) 110, 486, 689, 810
HapII CCGG 1 cut(s) 950
HgaI GACGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 31, 48
Hin6I GCGC 2 cut(s) 29, 46
HinP1I GCGC 2 cut(s) 29, 46
HincII GTYRAC 1 cut(s) 671
HindII GTYRAC 1 cut(s) 671
HinfI GANTC 6 cut(s) 65, 227, 264, 301, 409, 451
HpaII CCGG 1 cut(s) 950
HphI GGTGA 5 cut(s) 32, 719, 776, 1109, 1145
Hpy166II GTNNAC 6 cut(s) 327, 671, 725, 845, 957, 1231
Hpy188I TCNGA 6 cut(s) 101, 115, 349, 549, 1204, 1256
Hpy188III TCNNGA 9 cut(s) 362, 425, 434, 752, 818, 878, 968, 979, 1246
Hpy8I GTNNAC 6 cut(s) 327, 671, 725, 845, 957, 1231
HpyAV CCTTC 2 cut(s) 204, 1019
HpyCH4III ACNGT 3 cut(s) 431, 659, 764
HpyCH4V TGCA 2 cut(s) 344, 1124
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
HpyF3I CTNAG 3 cut(s) 98, 315, 321
HspAI GCGC 2 cut(s) 29, 46
Kzo9I GATC 5 cut(s) 22, 172, 766, 1199, 1248
LmnI GCTCC 4 cut(s) 56, 148, 156, 196
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 4 cut(s) 353, 769, 873, 1044
MaeI CTAG 3 cut(s) 26, 663, 818
MaeIII GTNAC 1 cut(s) 907
MalI GATC 5 cut(s) 24, 174, 768, 1201, 1250
MboI GATC 5 cut(s) 22, 172, 766, 1199, 1248
MboII GAAGA 7 cut(s) 74, 128, 812, 927, 956, 1175, 1243
MflI RGATCY 2 cut(s) 172, 1199
MhlI GDGCHC 2 cut(s) 145, 153
MluCI AATT 9 cut(s) 285, 307, 742, 828, 1002, 1162, 1179, 1207, 1239
MlyI GAGTC 3 cut(s) 74, 273, 295
MmeI TCCRAC 2 cut(s) 348, 777
MroXI GAANNNNTTC 2 cut(s) 231, 714
MseI TTAA 5 cut(s) 306, 333, 522, 741, 834
MslI CAYNNNNRTG 1 cut(s) 138
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 3 cut(s) 146, 406, 950
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 2 cut(s) 146, 406
MwoI GCNNNNNNNGC 3 cut(s) 350, 471, 1075
NciI CCSGG 1 cut(s) 950
NdeI CATATG 1 cut(s) 237
NdeII GATC 5 cut(s) 22, 172, 766, 1199, 1248
NlaIV GGNNCC 4 cut(s) 131, 174, 251, 885
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 2 cut(s) 210, 1012
PagI TCATGA 3 cut(s) 424, 751, 1245
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 2 cut(s) 231, 714
PfeI GAWTC 3 cut(s) 227, 409, 451
PfoI TCCNGGA 1 cut(s) 948
PkrI GCNGC 3 cut(s) 79, 622, 812
PleI GAGTC 3 cut(s) 73, 272, 295
PpsI GAGTC 3 cut(s) 73, 272, 295
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 144, 404
PspGI CCWGG 2 cut(s) 144, 404
PspN4I GGNNCC 4 cut(s) 131, 174, 251, 885
PspPI GGNCC 3 cut(s) 129, 249, 484
PstNI CAGNNNCTG 2 cut(s) 353, 1130
PsuI RGATCY 2 cut(s) 172, 1199
RsaI GTAC 7 cut(s) 661, 685, 726, 921, 986, 1020, 1146
RsaNI GTAC 7 cut(s) 660, 684, 725, 920, 985, 1019, 1145
RseI CAYNNNNRTG 1 cut(s) 138
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 5 cut(s) 306, 333, 522, 741, 834
SatI GCNGC 3 cut(s) 78, 621, 811
Sau3AI GATC 5 cut(s) 22, 172, 766, 1199, 1248
Sau96I GGNCC 3 cut(s) 129, 249, 484
ScaI AGTACT 1 cut(s) 986
SchI GAGTC 3 cut(s) 74, 273, 295
ScrFI CCNGG 3 cut(s) 146, 406, 950
SduI GDGCHC 2 cut(s) 145, 153
SfaNI GCATC 4 cut(s) 353, 769, 873, 1044
SinI GGWCC 2 cut(s) 129, 249
SmiMI CAYNNNNRTG 1 cut(s) 138
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 9 cut(s) 285, 307, 742, 828, 1002, 1162, 1179, 1207, 1239
SsiI CCGC 4 cut(s) 78, 223, 514, 811
SspMI CTAG 3 cut(s) 26, 663, 818
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 3 cut(s) 144, 404, 948
StyI CCWWGG 2 cut(s) 168, 245
TaaI ACNGT 3 cut(s) 431, 659, 764
TaqI TCGA 2 cut(s) 769, 1073
TaqII GACCGA 2 cut(s) 444, 928
TasI AATT 9 cut(s) 285, 307, 742, 828, 1002, 1162, 1179, 1207, 1239
TatI WGTACW 2 cut(s) 659, 984
TauI GCSGC 2 cut(s) 80, 813
TfiI GAWTC 3 cut(s) 227, 409, 451
Tru1I TTAA 5 cut(s) 306, 333, 522, 741, 834
Tru9I TTAA 5 cut(s) 306, 333, 522, 741, 834
TscAI CASTG 3 cut(s) 21, 907, 1085
TseI GCWGC 1 cut(s) 620
TspDTI ATGAA 3 cut(s) 224, 413, 740
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 3 cut(s) 21, 907, 1085
VpaK11BI GGWCC 2 cut(s) 129, 249
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 2 cut(s) 210, 1012
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 2 cut(s) 231, 714
XspI CTAG 3 cut(s) 26, 663, 818
ZrmI AGTACT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.