Rorug04G0071900

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006360
Physical Location & Seq
Forward (+)
11048173 .. 11050211
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0071900.1

Sequence Viewer

Length: 963 bp
ATGGGAAAGCTCTCCAAGGCCGAGAAGAAGATCAACTACGATGCCAAGCTCTGCCGGCTCCTCGACGACTACGCCCAGGTTCTCATCGTCGCCGCCGATAACGTCGGCTCCAATCAGCTCCAGAACATCCGCCAGGGCTTACGCGGCCACTCCATCGTTCTCATGGGGAAGAACACCATGATGAAGAGGTCCATCAAGATCCATGCTGAAAAGACCGGTAACAATGCTATTCTCAACCTCGTCCCCCTCCTCGTGGGTAATGTGGGTTTGATCTTCACCAAGGGGGATTTGAAGGAAGTGAAAGAAGAGGTTGCCAAGTACAAGGTTGGGGCCCCTGCTCGTGTGGGTTTGGTTGCTCCAGTTGATGTTGTTGTTCCTCCTGGCAACACTGGACTCGACCCATCCCAGACCTCTTTCTTCCAGGTGCTTAACATTCCAACTAAGATTAACAAGGGAACTGTGGAAATCATTACCCCTGTTGAACTTATTAAGAAGGGTGACAAGGTTGGGTCTTCCGAGTCTGCTCTGCTTGCCAAGCTCGGGATCAGGCCATTCTCTTATGGTCTTGTTGTCCAGATGGTGTATGAAAATGGTTCTGTTTTCAGCCCAGAGGTGCTTGATCTTACTGAGGATGATCTCGCGTTGACGTTTGCTTCTAATGTCTCCAATGTCGCAGCATTGGCATTGGCTATTTCCTACCCAACAATTGCAGCTGCCCCCCATATGTTCCTCAATGCCTACAAGAATGTGTTGGCAGTTGCTGTGGCTACTGAGTATTCCTTCCCTCAAGCAGATAAGGTCAAGGAGTATCTGAAGGATCCTACCAAGTTTGCTGCTCTGGCTGCCCCTGCAGCTGCTACTGGTGCTGCTGCTACCCCTACTGCGGCTGCTAAGGTGGAGGCGAAGAAGGAAGAGCCTTCTGAGGAATCAGATGAGGACATGGGTCTTGGTTTATTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

320

Amino Acids

34.01

Weight (kDa)

6.36

Isoelectric Point (pI)

29.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L10 PF00466 6 - 107 1.2e-17 Ribosomal protein L10
RL10P_insert PF17777 113 - 182 8.3e-21 Insertion domain in 60S ribosomal protein L10P
Ribosomal_60s PF00428 233 - 319 1.8e-21 60s Acidic ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 144, 641
AciI CCGC 4 cut(s) 93, 130, 144, 884
AclWI GGATC 4 cut(s) 193, 551, 812, 825
AcoI YGGCCR 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 833
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 3 cut(s) 253, 540, 883
AgeI ACCGGT 1 cut(s) 215
AgsI TTSAA 2 cut(s) 292, 482
AjnI CCWGG 4 cut(s) 75, 132, 379, 420
AluBI AGCT 6 cut(s) 10, 49, 118, 538, 713, 854
AluI AGCT 6 cut(s) 10, 49, 118, 538, 713, 854
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 4 cut(s) 193, 551, 812, 825
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 4 cut(s) 18, 145, 330, 548
ApaI GGGCCC 1 cut(s) 334
AsiGI ACCGGT 1 cut(s) 215
AspS9I GGNCC 3 cut(s) 189, 330, 331
AsuHPI GGTGA 2 cut(s) 268, 509
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 334
BamHI GGATCC 1 cut(s) 817
BanII GRGCYC 1 cut(s) 334
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BauI CACGAG 2 cut(s) 251, 339
BbsI GAAGAC 1 cut(s) 504
BccI CCATC 4 cut(s) 161, 200, 409, 571
BciT130I CCWGG 4 cut(s) 77, 134, 381, 422
BcoDI GTCTC 1 cut(s) 667
BfmI CTRYAG 1 cut(s) 849
Bme1390I CCNGG 4 cut(s) 77, 134, 381, 422
Bme18I GGWCC 1 cut(s) 189
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 3 cut(s) 189, 330, 331
BmiI GGNNCC 6 cut(s) 59, 109, 331, 332, 333, 819
BmrFI CCNGG 4 cut(s) 77, 134, 381, 422
BmsI GCATC 1 cut(s) 31
BoxI GACNNNNGTC 1 cut(s) 942
BpiI GAAGAC 1 cut(s) 504
BpmI CTGGAG 2 cut(s) 104, 342
Bpu10I CCTNAGC 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 771
BsaJI CCNNGG 4 cut(s) 15, 75, 133, 279
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 540, 883
Bse118I RCCGGY 2 cut(s) 54, 215
Bse1I ACTGG 3 cut(s) 359, 394, 865
BseBI CCWGG 4 cut(s) 77, 134, 381, 422
BseDI CCNNGG 4 cut(s) 15, 75, 133, 279
BseGI GGATG 3 cut(s) 126, 401, 637
BseLI CCNNNNNNNGG 3 cut(s) 253, 540, 883
BseMII CTCAG 3 cut(s) 618, 762, 912
BseNI ACTGG 3 cut(s) 359, 394, 865
BseRI GAGGAG 2 cut(s) 50, 239
BseSI GKGCMC 1 cut(s) 334
Bsh1236I CGCG 2 cut(s) 144, 641
BshFI GGCC 4 cut(s) 20, 147, 332, 550
BshTI ACCGGT 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 55, 216
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 253, 540, 883
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 4 cut(s) 20, 147, 332, 550
BsoBI CYCGRG 1 cut(s) 539
Bsp120I GGGCCC 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
BspACI CCGC 4 cut(s) 93, 130, 144, 884
BspANI GGCC 4 cut(s) 20, 147, 332, 550
BspCNI CTCAG 3 cut(s) 619, 763, 913
BspFNI CGCG 2 cut(s) 144, 641
BspLI GGNNCC 6 cut(s) 59, 109, 331, 332, 333, 819
BspMAI CTGCAG 1 cut(s) 853
BspPI GGATC 4 cut(s) 193, 551, 812, 825
BspQI GCTCTTC 1 cut(s) 906
BsrFI RCCGGY 2 cut(s) 54, 215
BsrI ACTGG 3 cut(s) 359, 394, 865
BssAI RCCGGY 2 cut(s) 54, 215
BssECI CCNNGG 4 cut(s) 15, 75, 133, 279
BssMI GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
BssSI CACGAG 2 cut(s) 251, 339
BssT1I CCWWGG 2 cut(s) 15, 279
Bst2BI CACGAG 2 cut(s) 251, 339
Bst2UI CCWGG 4 cut(s) 77, 134, 381, 422
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 3 cut(s) 179, 300, 906
BstC8I GCNNGC 2 cut(s) 56, 531
BstDEI CTNAG 5 cut(s) 441, 627, 771, 891, 921
BstF5I GGATG 3 cut(s) 126, 401, 637
BstFNI CGCG 2 cut(s) 144, 641
BstKTI GATC 7 cut(s) 33, 201, 273, 546, 622, 637, 820
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
BstNI CCWGG 4 cut(s) 77, 134, 381, 422
BstPAI GACNNNNGTC 1 cut(s) 942
BstSCI CCNGG 4 cut(s) 75, 132, 379, 420
BstSFI CTRYAG 1 cut(s) 849
BstSLI GKGCMC 1 cut(s) 334
BstUI CGCG 2 cut(s) 144, 641
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 2 cut(s) 198, 817
BstYI RGATCY 2 cut(s) 198, 817
BsuRI GGCC 4 cut(s) 20, 147, 332, 550
BtsCI GGATG 3 cut(s) 126, 401, 637
BtsIMutI CAGTG 1 cut(s) 387
Cac8I GCNNGC 2 cut(s) 56, 531
CaiI CAGNNNCTG 1 cut(s) 761
Cfr10I RCCGGY 2 cut(s) 54, 215
Cfr13I GGNCC 3 cut(s) 189, 330, 331
Csp6I GTAC 1 cut(s) 319
CspAI ACCGGT 1 cut(s) 215
CviAII CATG 4 cut(s) 163, 178, 203, 940
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 5 cut(s) 441, 627, 771, 891, 921
DpnI GATC 7 cut(s) 32, 200, 272, 545, 621, 636, 819
DpnII GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 3 cut(s) 179, 300, 906
EarI CTCTTC 3 cut(s) 179, 300, 906
EciI GGCGGA 1 cut(s) 119
Eco130I CCWWGG 2 cut(s) 15, 279
Eco24I GRGCYC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 539
EcoO109I RGGNCCY 2 cut(s) 330, 331
EcoRII CCWGG 4 cut(s) 75, 132, 379, 420
EcoT14I CCWWGG 2 cut(s) 15, 279
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 2 cut(s) 15, 279
FaeI CATG 4 cut(s) 166, 181, 206, 943
FaiI YATR 8 cut(s) 164, 179, 204, 561, 585, 723, 725, 941
FaqI GGGAC 1 cut(s) 227
FatI CATG 4 cut(s) 162, 177, 202, 939
FauNDI CATATG 1 cut(s) 723
FokI GGATG 3 cut(s) 113, 388, 644
FriOI GRGCYC 1 cut(s) 334
GsuI CTGGAG 2 cut(s) 104, 342
HaeIII GGCC 4 cut(s) 20, 147, 332, 550
HapII CCGG 2 cut(s) 55, 216
Hin1II CATG 4 cut(s) 166, 181, 206, 943
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HinfI GANTC 3 cut(s) 393, 518, 926
HpaII CCGG 2 cut(s) 55, 216
HphI GGTGA 2 cut(s) 268, 509
Hpy166II GTNNAC 1 cut(s) 645
Hpy188I TCNGA 4 cut(s) 517, 813, 922, 931
Hpy188III TCNNGA 4 cut(s) 121, 196, 541, 574
Hpy8I GTNNAC 1 cut(s) 645
Hpy99I CGWCG 3 cut(s) 68, 92, 107
HpyAV CCTTC 6 cut(s) 286, 487, 790, 808, 901, 927
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 2 cut(s) 102, 647
HpyCH4V TGCA 2 cut(s) 710, 851
HpyF3I CTNAG 5 cut(s) 441, 627, 771, 891, 921
HpySE526I ACGT 2 cut(s) 102, 647
Hsp92II CATG 4 cut(s) 166, 181, 206, 943
KroI GCCGGC 1 cut(s) 54
KroNI GCCGGC 1 cut(s) 56
Kzo9I GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 4 cut(s) 63, 113, 123, 361
LweI GCATC 1 cut(s) 31
MaeII ACGT 2 cut(s) 102, 647
MaeIII GTNAC 2 cut(s) 218, 497
MalI GATC 7 cut(s) 32, 200, 272, 545, 621, 636, 819
MboI GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 2 cut(s) 198, 817
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 1 cut(s) 705
MlyI GAGTC 2 cut(s) 387, 527
MmeI TCCRAC 1 cut(s) 461
MroNI GCCGGC 1 cut(s) 54
MseI TTAA 3 cut(s) 429, 447, 489
MslI CAYNNNNRTG 1 cut(s) 179
MspA1I CMGCKG 2 cut(s) 713, 854
MspI CCGG 2 cut(s) 55, 216
MspR9I CCNGG 4 cut(s) 77, 134, 381, 422
MunI CAATTG 1 cut(s) 705
MvaI CCWGG 4 cut(s) 77, 134, 381, 422
MvnI CGCG 2 cut(s) 144, 641
NaeI GCCGGC 1 cut(s) 56
NdeI CATATG 1 cut(s) 723
NdeII GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
NgoMIV GCCGGC 1 cut(s) 54
NlaIII CATG 4 cut(s) 166, 181, 206, 943
NlaIV GGNNCC 6 cut(s) 59, 109, 331, 332, 333, 819
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 906
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PdiI GCCGGC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 926
PinAI ACCGGT 1 cut(s) 215
PleI GAGTC 2 cut(s) 387, 526
PpsI GAGTC 2 cut(s) 387, 526
PshAI GACNNNNGTC 1 cut(s) 942
Psp6I CCWGG 4 cut(s) 75, 132, 379, 420
PspGI CCWGG 4 cut(s) 75, 132, 379, 420
PspN4I GGNNCC 6 cut(s) 59, 109, 331, 332, 333, 819
PspOMI GGGCCC 1 cut(s) 330
PspPI GGNCC 3 cut(s) 189, 330, 331
PstI CTGCAG 1 cut(s) 853
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 2 cut(s) 198, 817
PvuII CAGCTG 2 cut(s) 713, 854
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 3 cut(s) 429, 447, 489
Sau3AI GATC 7 cut(s) 30, 198, 270, 543, 619, 634, 817
Sau96I GGNCC 3 cut(s) 189, 330, 331
SchI GAGTC 2 cut(s) 387, 527
ScrFI CCNGG 4 cut(s) 77, 134, 381, 422
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 849
SinI GGWCC 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
Sse9I AATT 1 cut(s) 705
SsiI CCGC 4 cut(s) 93, 130, 144, 884
StyD4I CCNGG 4 cut(s) 75, 132, 379, 420
StyI CCWWGG 2 cut(s) 15, 279
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 2 cut(s) 105, 650
TaqI TCGA 3 cut(s) 63, 396, 957
TasI AATT 1 cut(s) 705
TatI WGTACW 1 cut(s) 318
TauI GCSGC 3 cut(s) 95, 147, 887
TfiI GAWTC 1 cut(s) 926
Tru1I TTAA 3 cut(s) 429, 447, 489
Tru9I TTAA 3 cut(s) 429, 447, 489
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 1 cut(s) 497
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 2 cut(s) 197, 600
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 189
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.