Rh4AG138800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006360
Physical Location & Seq
Reverse (-)
35392717 .. 35408015
15299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG138800.1

Sequence Viewer

Length: 1437 bp
ATGGGTTTGGAGGCAGTGGGTGATCTAGCGCTACACATAATCCTGCGCAAGTTAGGAGCTGAAGAGTCAGGGAGAGCCGCCTGCGTCAGCAAGCAACTCAGAGCTTCGGCCTCTGAAGAACCTCTTTGGTCCCATTTCTGTGCCCAGGAGCTCCACCTCTCGCAACCCTTGGATCCTCATGGCAACCTTGCTCCTTCTTTCAAGGCATGTTATCAATCATGGCGGGAATCTTTTCATATGTATCCTTGGTCCCTTGTCAAGCGAGTCAAAAGGTGTTGGGGCAAAATTACCAACTGGCTGACTCTTAATTTCCCAGAGGCTCAGTCTACGCTTAACAAAGGTGCATCAGAAGCTGACATTCAAGATTTGGAGAACAAATTGAAAGTAAAACTTCCCCTCCCAACCAGGATTCTCTATCGTTTTCATGACGGTCAAGATTTAGAGGACAAGAATTGGTCAAGTAGCCGAAATGGCACTCTACTGGGCCTCATAGGTGGTTATTCTTTTTATGACCGCATGGTTAATGTCTGCTTGTTGCCTTTACATCAGATAGTATTAGCAACAAAGGACATCATGGAAACGCTTGATTTTTCAGCAACACCGGATTATATTGTTGTGGCTGCTTCTACATTTAGCCAAAAGTTGTTTTTCCTCAACTGTACAAGTGGTCAACTCTATGTTGGTATGGCCAACCTTCTGCTTGATGGTGAAATGCTTCCTTGTGTACCGAATGCGTTGGTTAATTCAGTTCATGATTACAATGGTGGTGATCGACAGCAGGATGCCATGCTTCTTTGGTTGGAAGAACACGGCCGCCGTCTAGAAAATGGAATTATTAAACTCCGTGAACAAGAAACTTTGAGAAGCATCTGTCAGTTTCCAGAGGAACCCCCTTTCTGTTCCACTGCCGTAACAAGTGGCGTACAGGTTCGTTGTTCTTCGGTCTTTGTCCCGGAGTTTACTAATTTTCAAGATACCATCAGGAAGTACTTTTTTTCCTATTCAATTCGCATGTCGCTTGTACCTGAAGGATGTAGCATCAATGGAATGACATTCAACTCTTGCCAACTGCTTTCAAGGCACTGGGTCATTCGTGCTGGTGATGTTGTGGTAGGTAATGTAGATGCAGAGGCTGTTATAGGAAAGTTTGTTCCGTATAATCGGTTAGATGAGTCAGTCCCCATAAATATTTGGCTCAAAAATGTCTTTGAAGGGAATTTGCAATTGTGTAGGAGGCCATTGACGACATGCAATTGGACTGAGATTAGGAGTCAAAATAAGATGTCAGAGGTTAACCACCTGGACCAGAAGACAAAGATCAATAAAAAAGAGAGAATAGAGAAGCGAGTACCTTGGAGAGATCTATTGGAGAAGGATGGAGGTGTCGGTGAAGACGTCAATGCCGTGAAGAATCGGGCCGTAGTGAGCTTACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 F-box SMI1_KNR4

Protein Analysis

478

Amino Acids

54.19

Weight (kDa)

7.13

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF525 PF04379 324 - 382 5.5e-08 ApaG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1398
Acc16I TGCGCA 1 cut(s) 47
AccI GTMKAC 1 cut(s) 326
AciI CCGC 4 cut(s) 78, 223, 514, 814
AclWI GGATC 2 cut(s) 167, 180
AcoI YGGCCR 2 cut(s) 687, 811
AcsI RAATTY 1 cut(s) 1216
AcuI CTGAAG 3 cut(s) 81, 135, 1047
AcyI GRCGYC 1 cut(s) 1395
AfaI GTAC 6 cut(s) 661, 726, 924, 989, 1023, 1350
AfeI AGCGCT 1 cut(s) 30
AgsI TTSAA 8 cut(s) 202, 362, 382, 971, 1005, 1057, 1077, 1211
AjnI CCWGG 3 cut(s) 144, 404, 1299
AloI GAACNNNNNNTCC 2 cut(s) 1134, 1166
AluBI AGCT 5 cut(s) 59, 104, 151, 353, 1428
AluI AGCT 5 cut(s) 59, 104, 151, 353, 1428
Alw21I GWGCWC 1 cut(s) 153
AlwI GGATC 2 cut(s) 167, 180
AlwNI CAGNNNCTG 2 cut(s) 353, 1133
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 6 cut(s) 108, 484, 687, 811, 1235, 1416
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 1 cut(s) 1216
Asp700I GAANNNNTTC 2 cut(s) 231, 714
AspLEI GCGC 2 cut(s) 31, 48
AspS9I GGNCC 5 cut(s) 129, 249, 484, 1303, 1416
AsuC2I CCSGG 1 cut(s) 953
AsuHPI GGTGA 5 cut(s) 32, 719, 779, 1112, 1400
AvaII GGWCC 3 cut(s) 129, 249, 1303
BaeGI GKGCMC 1 cut(s) 145
BalI TGGCCA 1 cut(s) 689
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 153
BbsI GAAGAC 2 cut(s) 1316, 1398
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 1 cut(s) 607
BccI CCATC 3 cut(s) 698, 986, 1370
BceAI ACGGC 5 cut(s) 801, 826, 893, 1388, 1403
BciT130I CCWGG 3 cut(s) 146, 406, 1301
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 953
BfaI CTAG 2 cut(s) 26, 821
BfoI RGCGCY 1 cut(s) 32
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 471
BglII AGATCT 1 cut(s) 1360
BisI GCNGC 3 cut(s) 78, 621, 814
BlsI GCNGC 3 cut(s) 79, 622, 815
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 4 cut(s) 146, 406, 953, 1301
Bme18I GGWCC 3 cut(s) 129, 249, 1303
BmgT120I GGNCC 5 cut(s) 129, 249, 484, 1303, 1416
BmiI GGNNCC 4 cut(s) 131, 174, 251, 888
BmrFI CCNGG 4 cut(s) 146, 406, 953, 1301
BmrI ACTGGG 2 cut(s) 491, 1093
BmsI GCATC 5 cut(s) 353, 772, 876, 1047, 1114
BmuI ACTGGG 2 cut(s) 491, 1093
BpiI GAAGAC 2 cut(s) 1316, 1398
BpuMI CCSGG 1 cut(s) 953
BsaHI GRCGYC 1 cut(s) 1395
BsaJI CCNNGG 4 cut(s) 144, 168, 245, 1352
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bse1I ACTGG 3 cut(s) 299, 486, 1088
BseBI CCWGG 3 cut(s) 146, 406, 1301
BseDI CCNNGG 4 cut(s) 144, 168, 245, 1352
BseGI GGATG 3 cut(s) 787, 1037, 1381
BseMII CTCAG 3 cut(s) 112, 335, 1251
BseNI ACTGG 3 cut(s) 299, 486, 1088
BseSI GKGCMC 1 cut(s) 145
BseX3I CGGCCG 1 cut(s) 811
BseXI GCAGC 1 cut(s) 607
Bsh1285I CGRYCG 1 cut(s) 814
BshFI GGCC 6 cut(s) 110, 486, 689, 813, 1237, 1418
BsiEI CGRYCG 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 2 cut(s) 602, 953
BslFI GGGAC 4 cut(s) 115, 235, 935, 1163
BsmFI GGGAC 4 cut(s) 115, 235, 935, 1163
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 6 cut(s) 110, 486, 689, 813, 1237, 1418
Bsp1286I GDGCHC 2 cut(s) 145, 153
Bsp1407I TGTACA 1 cut(s) 659
Bsp143I GATC 5 cut(s) 22, 172, 769, 1317, 1360
BspACI CCGC 4 cut(s) 78, 223, 514, 814
BspANI GGCC 6 cut(s) 110, 486, 689, 813, 1237, 1418
BspCNI CTCAG 3 cut(s) 111, 334, 1252
BspHI TCATGA 2 cut(s) 424, 751
BspLI GGNNCC 4 cut(s) 131, 174, 251, 888
BspPI GGATC 2 cut(s) 167, 180
BsrGI TGTACA 1 cut(s) 659
BsrI ACTGG 3 cut(s) 299, 486, 1088
BssECI CCNNGG 4 cut(s) 144, 168, 245, 1352
BssMI GATC 5 cut(s) 22, 172, 769, 1317, 1360
BssNI GRCGYC 1 cut(s) 1395
BssT1I CCWWGG 3 cut(s) 168, 245, 1352
Bst2UI CCWGG 3 cut(s) 146, 406, 1301
Bst4CI ACNGT 3 cut(s) 431, 659, 1434
Bst6I CTCTTC 1 cut(s) 57
BstACI GRCGYC 1 cut(s) 1395
BstAUI TGTACA 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 82, 92
BstDEI CTNAG 3 cut(s) 98, 321, 1260
BstF5I GGATG 3 cut(s) 787, 1037, 1381
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 48
BstKTI GATC 5 cut(s) 25, 175, 772, 1320, 1363
BstMBI GATC 5 cut(s) 22, 172, 769, 1317, 1360
BstMCI CGRYCG 1 cut(s) 814
BstMWI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
BstNI CCWGG 3 cut(s) 146, 406, 1301
BstNSI RCATGY 3 cut(s) 210, 1015, 1251
BstSCI CCNGG 4 cut(s) 144, 404, 951, 1299
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 2 cut(s) 1316, 1398
BstX2I RGATCY 2 cut(s) 172, 1360
BstYI RGATCY 2 cut(s) 172, 1360
BstZI CGGCCG 1 cut(s) 811
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 6 cut(s) 110, 486, 689, 813, 1237, 1418
BtsCI GGATG 3 cut(s) 787, 1037, 1381
BtsI GCAGTG 2 cut(s) 21, 903
BtsIMutI CAGTG 3 cut(s) 21, 903, 1081
Cac8I GCNNGC 2 cut(s) 82, 92
CaiI CAGNNNCTG 2 cut(s) 353, 1133
CciI TCATGA 2 cut(s) 424, 751
CfoI GCGC 2 cut(s) 31, 48
Cfr13I GGNCC 5 cut(s) 129, 249, 484, 1303, 1416
CseI GACGC 1 cut(s) 73
Csp6I GTAC 6 cut(s) 660, 725, 923, 988, 1022, 1349
CviQI GTAC 6 cut(s) 660, 725, 923, 988, 1022, 1349
DdeI CTNAG 3 cut(s) 98, 321, 1260
DpnI GATC 5 cut(s) 24, 174, 771, 1319, 1362
DpnII GATC 5 cut(s) 22, 172, 769, 1317, 1360
EaeI YGGCCR 2 cut(s) 687, 811
EagI CGGCCG 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 151
EclXI CGGCCG 1 cut(s) 811
Eco130I CCWWGG 3 cut(s) 168, 245, 1352
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 3 cut(s) 129, 249, 1303
Eco47III AGCGCT 1 cut(s) 30
Eco52I CGGCCG 1 cut(s) 811
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 3 cut(s) 81, 135, 1047
EcoICRI GAGCTC 1 cut(s) 151
EcoRII CCWGG 3 cut(s) 144, 404, 1299
EcoT14I CCWWGG 3 cut(s) 168, 245, 1352
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 3 cut(s) 168, 245, 1352
FalI AAGNNNNNCTT 4 cut(s) 108, 140, 375, 407
FaqI GGGAC 4 cut(s) 115, 235, 935, 1163
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 237
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 78, 621, 814
FokI GGATG 3 cut(s) 794, 1044, 1388
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 3 cut(s) 78, 621, 814
FspBI CTAG 2 cut(s) 26, 821
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 30, 47
GluI GCNGC 3 cut(s) 78, 621, 814
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 6 cut(s) 110, 486, 689, 813, 1237, 1418
HapII CCGG 2 cut(s) 602, 953
HgaI GACGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 31, 48
Hin1I GRCGYC 1 cut(s) 1395
Hin6I GCGC 2 cut(s) 29, 46
HinP1I GCGC 2 cut(s) 29, 46
HincII GTYRAC 2 cut(s) 671, 1294
HindII GTYRAC 2 cut(s) 671, 1294
HinfI GANTC 8 cut(s) 65, 227, 264, 301, 409, 1172, 1270, 1411
HpaI GTTAAC 1 cut(s) 1294
HpaII CCGG 2 cut(s) 602, 953
HphI GGTGA 5 cut(s) 32, 719, 779, 1112, 1400
Hpy166II GTNNAC 6 cut(s) 327, 671, 725, 848, 960, 1294
Hpy188I TCNGA 5 cut(s) 101, 115, 349, 549, 1288
Hpy188III TCNNGA 8 cut(s) 362, 425, 434, 752, 821, 881, 971, 982
Hpy8I GTNNAC 6 cut(s) 327, 671, 725, 848, 960, 1294
HpyAV CCTTC 5 cut(s) 204, 704, 1022, 1205, 1366
HpyCH4III ACNGT 3 cut(s) 431, 659, 1434
HpyCH4IV ACGT 1 cut(s) 1395
HpyCH4V TGCA 4 cut(s) 344, 1127, 1222, 1251
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
HpyF3I CTNAG 3 cut(s) 98, 321, 1260
HpySE526I ACGT 1 cut(s) 1395
Hsp92I GRCGYC 1 cut(s) 1395
HspAI GCGC 2 cut(s) 29, 46
KspAI GTTAAC 1 cut(s) 1294
Kzo9I GATC 5 cut(s) 22, 172, 769, 1317, 1360
LmnI GCTCC 4 cut(s) 56, 148, 156, 196
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 5 cut(s) 353, 772, 876, 1047, 1114
MaeI CTAG 2 cut(s) 26, 821
MaeII ACGT 1 cut(s) 1395
MaeIII GTNAC 1 cut(s) 910
MalI GATC 5 cut(s) 24, 174, 771, 1319, 1362
MboI GATC 5 cut(s) 22, 172, 769, 1317, 1360
MboII GAAGA 7 cut(s) 74, 128, 815, 930, 1321, 1403, 1420
MfeI CAATTG 2 cut(s) 1223, 1252
MflI RGATCY 2 cut(s) 172, 1360
MhlI GDGCHC 2 cut(s) 145, 153
MlsI TGGCCA 1 cut(s) 689
MluNI TGGCCA 1 cut(s) 689
MlyI GAGTC 5 cut(s) 74, 273, 295, 1181, 1279
MmeI TCCRAC 1 cut(s) 780
Mox20I TGGCCA 1 cut(s) 689
MroXI GAANNNNTTC 2 cut(s) 231, 714
MscI TGGCCA 1 cut(s) 689
MseI TTAA 6 cut(s) 306, 333, 522, 741, 837, 1293
MslI CAYNNNNRTG 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 689
MspI CCGG 2 cut(s) 602, 953
MspR9I CCNGG 4 cut(s) 146, 406, 953, 1301
MunI CAATTG 2 cut(s) 1223, 1252
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 3 cut(s) 146, 406, 1301
MwoI GCNNNNNNNGC 3 cut(s) 350, 471, 1078
NciI CCSGG 1 cut(s) 953
NdeI CATATG 1 cut(s) 237
NdeII GATC 5 cut(s) 22, 172, 769, 1317, 1360
NlaIV GGNNCC 4 cut(s) 131, 174, 251, 888
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 3 cut(s) 210, 1015, 1251
PagI TCATGA 2 cut(s) 424, 751
PcsI WCGNNNNNNNCGW 2 cut(s) 1392, 1401
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 2 cut(s) 231, 714
PfeI GAWTC 3 cut(s) 227, 409, 1411
PfoI TCCNGGA 1 cut(s) 951
PkrI GCNGC 3 cut(s) 79, 622, 815
PleI GAGTC 5 cut(s) 73, 272, 295, 1180, 1278
PpsI GAGTC 5 cut(s) 73, 272, 295, 1180, 1278
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 144, 404, 1299
PspGI CCWGG 3 cut(s) 144, 404, 1299
PspN4I GGNNCC 4 cut(s) 131, 174, 251, 888
PspPI GGNCC 5 cut(s) 129, 249, 484, 1303, 1416
PstNI CAGNNNCTG 2 cut(s) 353, 1133
PsuI RGATCY 2 cut(s) 172, 1360
RsaI GTAC 6 cut(s) 661, 726, 924, 989, 1023, 1350
RsaNI GTAC 6 cut(s) 660, 725, 923, 988, 1022, 1349
RseI CAYNNNNRTG 1 cut(s) 138
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 6 cut(s) 306, 333, 522, 741, 837, 1293
SatI GCNGC 3 cut(s) 78, 621, 814
Sau3AI GATC 5 cut(s) 22, 172, 769, 1317, 1360
Sau96I GGNCC 5 cut(s) 129, 249, 484, 1303, 1416
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 5 cut(s) 74, 273, 295, 1181, 1279
ScrFI CCNGG 4 cut(s) 146, 406, 953, 1301
SduI GDGCHC 2 cut(s) 145, 153
SfaNI GCATC 5 cut(s) 353, 772, 876, 1047, 1114
SinI GGWCC 3 cut(s) 129, 249, 1303
SmiMI CAYNNNNRTG 1 cut(s) 138
SsiI CCGC 4 cut(s) 78, 223, 514, 814
SspI AATATT 1 cut(s) 1189
SspMI CTAG 2 cut(s) 26, 821
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 4 cut(s) 144, 404, 951, 1299
StyI CCWWGG 3 cut(s) 168, 245, 1352
TaaI ACNGT 3 cut(s) 431, 659, 1434
TaiI ACGT 1 cut(s) 1398
TaqI TCGA 1 cut(s) 772
TaqII GACCGA 1 cut(s) 931
TatI WGTACW 2 cut(s) 659, 987
TauI GCSGC 2 cut(s) 80, 816
TfiI GAWTC 3 cut(s) 227, 409, 1411
Tru1I TTAA 6 cut(s) 306, 333, 522, 741, 837, 1293
Tru9I TTAA 6 cut(s) 306, 333, 522, 741, 837, 1293
TscAI CASTG 3 cut(s) 21, 910, 1088
TseI GCWGC 1 cut(s) 620
TspDTI ATGAA 3 cut(s) 224, 413, 740
TspGWI ACGGA 2 cut(s) 833, 1143
TspRI CASTG 3 cut(s) 21, 910, 1088
VpaK11BI GGWCC 3 cut(s) 129, 249, 1303
XapI RAATTY 1 cut(s) 1216
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 3 cut(s) 210, 1015, 1251
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 2 cut(s) 231, 714
XspI CTAG 2 cut(s) 26, 821
ZraI GACGTC 1 cut(s) 1396
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.