FvH4_4g15450

5'-nucleotidase surE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006194
Physical Location & Seq
Reverse (-)
19115676 .. 19121088
5413 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15450.t1

Sequence Viewer

Length: 921 bp
ATGGCAAACAACATAAACACCGAAAAGCCCACCGTGATGGTCACCAACGACGACGGAATCGAAGGCCCCGGACTTCTCTCCCTCGTCCACGTCCTCGTCTCCACCAATCTCTACAATGTTCTCGTCTGCGCTCCCGACTCGGAAAACTCTGCTGTCAGTCACTGCATCACTTGGCGACGTCCGATTGCCGCCAAAATAGTCGACATCGCCGGAGCCACGGCCTTTGCAGTTTCCGGGACTCCTGCTGATTGTGCTTCTTTGGGTGTTTCTGAAGCACTGTTTCCTTCTGTTCCCGACTTGGTGATCAGCGGCATAAACAAGGGCAGTAACTGTGGTTATCACATTACTTACTCTGGTACCGTGGCTGGCGCTCGGGAAGCTTTCTTTAATGGTGTACCTGCTGTATCTGTATCTTATGATTGGGTTGAAGGCAAGAGTCGCATTAATTACTTCACACTTGCTGCTGAAGCTTGCTTACCTATCATAAATGCAATACTCGTGGAAATAAAAAATCAAACCTTTCCTCAAGGATGTTTTCTGAATGTAGATTTGCCAACGGACCTTGCTACTCATAAGGGGTATAAGCTGACTAAACAGGGTAACAGTATAATCAAAATGAAGTGGAAACAAGTTACTTCAAAGATGCCAAAAATGTTGTCATCGATGGAAATGGAGAAAGATTCAGCAGCATCTACTGGAATTGATACATCAACTACGCCAAAAGAAGATCCTTTGTTCGCAAGAGAGGTAATTGGTTTCCGACATGATAGTGATGATGATACCGACCACAAATGTGTTCAAGAAGGATATATTACTGTCACTCCTCTAGGTGCGTTCACTAATGCCGAGATAGATTGCAGCACCTACTTAAAAGAGTGGCTTCCAAATGTAGCTGACCGCACCTCTTCATCTGCCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
SurE

Protein Analysis

307

Amino Acids

32.88

Weight (kDa)

5.06

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SurE PF01975 12 - 203 1.6e-52 Survival protein SurE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 181
Acc36I ACCTGC 1 cut(s) 406
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 201
AciI CCGC 3 cut(s) 189, 309, 898
AclWI GGATC 1 cut(s) 722
AcuI CTGAAG 2 cut(s) 291, 486
AcyI GRCGYC 1 cut(s) 178
AfaI GTAC 2 cut(s) 358, 396
AgsI TTSAA 3 cut(s) 428, 639, 800
AjiI CACGTC 1 cut(s) 91
AleI CACNNNNGTG 1 cut(s) 792
AluBI AGCT 4 cut(s) 380, 470, 586, 893
AluI AGCT 4 cut(s) 380, 470, 586, 893
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 1 cut(s) 722
AlwNI CAGNNNCTG 2 cut(s) 162, 330
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 2 cut(s) 64, 219
ApeKI GCWGC 3 cut(s) 461, 686, 858
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
AseI ATTAAT 1 cut(s) 444
Asp718I GGTACC 1 cut(s) 356
AspLEI GCGC 2 cut(s) 131, 371
AspS9I GGNCC 2 cut(s) 65, 559
AsuC2I CCSGG 2 cut(s) 69, 235
AsuHPI GGTGA 2 cut(s) 34, 313
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 559
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BanI GGYRCC 1 cut(s) 356
BarI GAAGNNNNNNTAC 2 cut(s) 459, 491
BauI CACGAG 1 cut(s) 497
BbvI GCAGC 3 cut(s) 448, 698, 870
BccI CCATC 2 cut(s) 31, 658
BceAI ACGGC 1 cut(s) 234
BclI TGATCA 1 cut(s) 303
BcnI CCSGG 2 cut(s) 69, 235
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 1 cut(s) 827
BfoI RGCGCY 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 406
BisI GCNGC 5 cut(s) 189, 310, 462, 687, 859
BlsI GCNGC 5 cut(s) 190, 311, 463, 688, 860
Bme1390I CCNGG 2 cut(s) 69, 235
Bme18I GGWCC 1 cut(s) 559
BmeT110I CYCGRG 1 cut(s) 372
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 65, 559
BmiI GGNNCC 3 cut(s) 67, 214, 358
BmrFI CCNGG 2 cut(s) 69, 235
BmsI GCATC 3 cut(s) 174, 633, 698
BpuEI CTTGAG 1 cut(s) 510
BpuMI CCSGG 2 cut(s) 69, 235
Bsa29I ATCGAT 1 cut(s) 662
BsaHI GRCGYC 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 67, 216, 360
BsaXI ACNNNNNCTCC 2 cut(s) 805, 835
Bse1I ACTGG 1 cut(s) 700
BseCI ATCGAT 1 cut(s) 662
BseDI CCNNGG 3 cut(s) 67, 216, 360
BseGI GGATG 1 cut(s) 536
BseNI ACTGG 1 cut(s) 700
BseRI GAGGAG 1 cut(s) 813
BseXI GCAGC 3 cut(s) 448, 698, 870
BshFI GGCC 2 cut(s) 66, 221
BshNI GGYRCC 1 cut(s) 356
BshVI ATCGAT 1 cut(s) 662
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 3 cut(s) 69, 210, 234
BslFI GGGAC 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 103
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 2 cut(s) 66, 221
BsoBI CYCGRG 1 cut(s) 372
Bsp143I GATC 2 cut(s) 303, 727
BspACI CCGC 3 cut(s) 189, 309, 898
BspANI GGCC 2 cut(s) 66, 221
BspDI ATCGAT 1 cut(s) 662
BspLI GGNNCC 3 cut(s) 67, 214, 358
BspMI ACCTGC 1 cut(s) 406
BspPI GGATC 1 cut(s) 722
BspT107I GGYRCC 1 cut(s) 356
BsrI ACTGG 1 cut(s) 700
BssECI CCNNGG 3 cut(s) 67, 216, 360
BssMI GATC 2 cut(s) 303, 727
BssNI GRCGYC 1 cut(s) 178
BssSI CACGAG 1 cut(s) 497
Bst2BI CACGAG 1 cut(s) 497
Bst4CI ACNGT 6 cut(s) 34, 279, 332, 361, 605, 817
Bst6I CTCTTC 1 cut(s) 910
BstACI GRCGYC 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 367, 472
BstDSI CCRYGG 2 cut(s) 216, 360
BstEII GGTNACC 1 cut(s) 40
BstF5I GGATG 1 cut(s) 536
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 131, 371
BstKTI GATC 2 cut(s) 306, 730
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 2 cut(s) 303, 727
BstMWI GCNNNNNNNGC 4 cut(s) 251, 377, 438, 467
BstPI GGTNACC 1 cut(s) 40
BstSCI CCNGG 2 cut(s) 67, 233
BstV1I GCAGC 3 cut(s) 448, 698, 870
BstX2I RGATCY 1 cut(s) 727
BstXI CCANNNNNNTGG 1 cut(s) 37
BstYI RGATCY 1 cut(s) 727
Bsu15I ATCGAT 1 cut(s) 662
BsuRI GGCC 2 cut(s) 66, 221
BsuTUI ATCGAT 1 cut(s) 662
BtgI CCRYGG 2 cut(s) 216, 360
BtgZI GCGATG 1 cut(s) 190
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 1 cut(s) 536
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 2 cut(s) 160, 275
BveI ACCTGC 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 367, 472
CaiI CAGNNNCTG 2 cut(s) 162, 330
CfoI GCGC 2 cut(s) 131, 371
Cfr13I GGNCC 2 cut(s) 65, 559
ClaI ATCGAT 1 cut(s) 662
Csp6I GTAC 2 cut(s) 357, 395
CspCI CAANNNNNGTGG 4 cut(s) 205, 240, 480, 515
CviAII CATG 1 cut(s) 764
CviQI GTAC 2 cut(s) 357, 395
DpnI GATC 2 cut(s) 305, 729
DpnII GATC 2 cut(s) 303, 727
Eam1104I CTCTTC 1 cut(s) 910
EarI CTCTTC 1 cut(s) 910
Eco47I GGWCC 1 cut(s) 559
Eco57I CTGAAG 2 cut(s) 291, 486
Eco88I CYCGRG 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 65
EcoO65I GGTNACC 1 cut(s) 40
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 1 cut(s) 767
FalI AAGNNNNNCTT 2 cut(s) 864, 896
FaqI GGGAC 1 cut(s) 250
FatI CATG 1 cut(s) 763
FbaI TGATCA 1 cut(s) 303
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 5 cut(s) 189, 310, 462, 687, 859
FokI GGATG 1 cut(s) 543
Fsp4HI GCNGC 5 cut(s) 189, 310, 462, 687, 859
FspBI CTAG 1 cut(s) 827
GlaI GCGC 2 cut(s) 130, 370
GluI GCNGC 5 cut(s) 189, 310, 462, 687, 859
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 2 cut(s) 66, 221
HapII CCGG 3 cut(s) 69, 210, 234
HhaI GCGC 2 cut(s) 131, 371
Hin1I GRCGYC 1 cut(s) 178
Hin1II CATG 1 cut(s) 767
Hin6I GCGC 2 cut(s) 129, 369
HinP1I GCGC 2 cut(s) 129, 369
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 2 cut(s) 378, 468
HinfI GANTC 5 cut(s) 57, 137, 238, 436, 680
HpaII CCGG 3 cut(s) 69, 210, 234
HphI GGTGA 2 cut(s) 34, 313
Hpy166II GTNNAC 4 cut(s) 88, 202, 395, 837
Hpy188I TCNGA 5 cut(s) 142, 183, 271, 540, 761
Hpy188III TCNNGA 4 cut(s) 134, 293, 374, 800
Hpy8I GTNNAC 4 cut(s) 88, 202, 395, 837
Hpy99I CGWCG 3 cut(s) 53, 56, 180
HpyAV CCTTC 4 cut(s) 56, 294, 422, 797
HpyCH4III ACNGT 6 cut(s) 34, 279, 332, 361, 605, 817
HpyCH4IV ACGT 2 cut(s) 90, 178
HpyCH4V TGCA 4 cut(s) 165, 227, 491, 858
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 377, 438, 467
HpySE526I ACGT 2 cut(s) 90, 178
Hsp92I GRCGYC 1 cut(s) 178
Hsp92II CATG 1 cut(s) 767
HspAI GCGC 2 cut(s) 129, 369
KpnI GGTACC 1 cut(s) 360
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 2 cut(s) 303, 727
LmnI GCTCC 2 cut(s) 136, 212
LpnPI CCDG 9 cut(s) 82, 223, 247, 255, 339, 351, 411, 581, 681
Lsp1109I GCAGC 3 cut(s) 448, 698, 870
LweI GCATC 3 cut(s) 174, 633, 698
MaeI CTAG 1 cut(s) 827
MaeII ACGT 2 cut(s) 90, 178
MaeIII GTNAC 6 cut(s) 40, 158, 326, 599, 631, 817
MalI GATC 2 cut(s) 305, 729
MboI GATC 2 cut(s) 303, 727
MboII GAAGA 2 cut(s) 737, 897
MflI RGATCY 1 cut(s) 727
MluCI AATT 3 cut(s) 445, 699, 750
MlyI GAGTC 3 cut(s) 131, 232, 445
MmeI TCCRAC 1 cut(s) 784
MnlI CCTC 6 cut(s) 92, 104, 534, 739, 834, 913
MseI TTAA 3 cut(s) 387, 444, 869
MslI CAYNNNNRTG 3 cut(s) 35, 768, 792
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 3 cut(s) 69, 210, 234
MspR9I CCNGG 2 cut(s) 69, 235
MwoI GCNNNNNNNGC 4 cut(s) 251, 377, 438, 467
NciI CCSGG 2 cut(s) 69, 235
NdeII GATC 2 cut(s) 303, 727
NlaIII CATG 1 cut(s) 767
NlaIV GGNNCC 3 cut(s) 67, 214, 358
NmeAIII GCCGAG 1 cut(s) 871
NmuCI GTSAC 3 cut(s) 40, 158, 817
OliI CACNNNNGTG 1 cut(s) 792
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 2 cut(s) 57, 680
PfoI TCCNGGA 1 cut(s) 233
PkrI GCNGC 5 cut(s) 190, 311, 463, 688, 860
PleI GAGTC 3 cut(s) 131, 232, 444
PpsI GAGTC 3 cut(s) 131, 232, 444
PshBI ATTAAT 1 cut(s) 444
PspEI GGTNACC 1 cut(s) 40
PspN4I GGNNCC 3 cut(s) 67, 214, 358
PspPI GGNCC 2 cut(s) 65, 559
PstNI CAGNNNCTG 2 cut(s) 162, 330
PsuI RGATCY 1 cut(s) 727
RsaI GTAC 2 cut(s) 358, 396
RsaNI GTAC 2 cut(s) 357, 395
RseI CAYNNNNRTG 3 cut(s) 35, 768, 792
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 3 cut(s) 387, 444, 869
SatI GCNGC 5 cut(s) 189, 310, 462, 687, 859
Sau3AI GATC 2 cut(s) 303, 727
Sau96I GGNCC 2 cut(s) 65, 559
SchI GAGTC 3 cut(s) 131, 232, 445
ScrFI CCNGG 2 cut(s) 69, 235
SfaNI GCATC 3 cut(s) 174, 633, 698
SinI GGWCC 1 cut(s) 559
SmiMI CAYNNNNRTG 3 cut(s) 35, 768, 792
SmlI CTYRAG 1 cut(s) 525
SmoI CTYRAG 1 cut(s) 525
Sse9I AATT 3 cut(s) 445, 699, 750
SsiI CCGC 3 cut(s) 189, 309, 898
SspMI CTAG 1 cut(s) 827
StyD4I CCNGG 2 cut(s) 67, 233
TaaI ACNGT 6 cut(s) 34, 279, 332, 361, 605, 817
TaiI ACGT 2 cut(s) 93, 181
TaqI TCGA 3 cut(s) 60, 201, 662
TasI AATT 3 cut(s) 445, 699, 750
TauI GCSGC 2 cut(s) 191, 312
TfiI GAWTC 2 cut(s) 57, 680
Tru1I TTAA 3 cut(s) 387, 444, 869
Tru9I TTAA 3 cut(s) 387, 444, 869
TscAI CASTG 2 cut(s) 167, 282
TseFI GTSAC 3 cut(s) 40, 158, 817
TseI GCWGC 3 cut(s) 461, 686, 858
Tsp45I GTSAC 3 cut(s) 40, 158, 817
TspDTI ATGAA 2 cut(s) 632, 897
TspGWI ACGGA 2 cut(s) 69, 572
TspRI CASTG 2 cut(s) 167, 282
VpaK11BI GGWCC 1 cut(s) 559
VspI ATTAAT 1 cut(s) 444
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 1 cut(s) 827
ZraI GACGTC 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.