MD13G1192300.v1.1

5'-nucleotidase surE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006194
Physical Location & Seq
Reverse (-)
16553217 .. 16557833
4617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1192300.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGATGTGCCCAACACTGTTGCCGTTCATACTTCCGTATTTTACTCTTGCTCTATCATTAGCAGTCTATTTTCCTCCAAATTCCGAAACCAAAACTCAGAGAAACAAGGAAATGGAAAACGCCACTGTCATCTCCGGCGACAAGCCCACCGTGATGGTGACCAACGACGACGGAATTGAAGGGCCAGGACTTCTCGCTCTCGTCCAAGTCCTCGTCTCCACCCATCGCTACAATGTTCTCGTCTGTGCCCCCGACTCGGAGAAGTCAGCAGTGAGTCACTGCATCACATGGCGCCATCCGATTGCTGCCAAGAAAGTGCATATCGATGGAGCAACAGCGTATGCTGTTTCTGGATCTCCAGCTGATTGTGCTTCTTTGGGTGTTTCCAAAACGCTTTTTCCGACAATTCCTGACCTGGTAATCAGTGGTATAAACATGGGCAGCAACTGCGGTTATCACATTGTTTACTCTGGAACGGTGGCTGGTGCTCGCGAGGCCTTTTTCAATGGTGTGCCTGCAATATCTATATCATATGATTGGGTTGGAGGTAAGAGCAATATTAATGATTTCACAATTGCTGCCGAGGCTTGCTTACCCATCATAAATGGAGTACTGGTGGAATTAAGAAACAAAACCTATCCTCAAAGATATTTCTTGAATGTAGATCTGCCATGTGATGTTGCTAATCATAAGGGGTATAAGCTGACTAAGCAAGGTAAAAGCATAATAAAAATGGGGTGGAGGCAAGTTACTTCTGACTCGGAAGGCGGGAAAATGTTGTCAACAATGGTAAATTCGATTGCATCAGCTGAAATTGCTACATCAACCACACCAGCTGAAAATACTTATTTCGTGAGAGAAGTAAGGGGTGCCCAAGCTGATGAGGATGATAATGACCACCGCTTTCTTCAAGAAGGATATATTACTGTCACTCCTCTTGGTGCCATCTCTAATGCTGAGATAGAATGCCAGACCTACTTAAAAGAGTGGCTTCCAACCGTGGTTGAGCGCCCCTCTCCATCGGCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
SurE

Protein Analysis

344

Amino Acids

37.14

Weight (kDa)

5.53

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SurE PF01975 51 - 242 1.3e-53 Survival protein SurE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1030
AccB1I GGYRCC 3 cut(s) 291, 869, 941
AccII CGCG 1 cut(s) 492
AciI CCGC 3 cut(s) 450, 768, 901
AclWI GGATC 1 cut(s) 361
AcsI RAATTY 2 cut(s) 79, 793
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 1 cut(s) 612
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 4 cut(s) 179, 505, 658, 911
AjnI CCWGG 2 cut(s) 184, 414
AluBI AGCT 5 cut(s) 362, 703, 809, 836, 878
AluI AGCT 5 cut(s) 362, 703, 809, 836, 878
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 1 cut(s) 361
AlwNI CAGNNNCTG 1 cut(s) 447
AoxI GGCC 2 cut(s) 182, 495
ApeKI GCWGC 3 cut(s) 305, 441, 578
ApoI RAATTY 2 cut(s) 79, 793
AseI ATTAAT 1 cut(s) 561
AspLEI GCGC 2 cut(s) 294, 1011
AspS9I GGNCC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 169
BaeGI GKGCMC 3 cut(s) 11, 250, 874
BanI GGYRCC 3 cut(s) 291, 869, 941
Bbv12I GWGCWC 1 cut(s) 490
BbvI GCAGC 3 cut(s) 292, 453, 565
BccI CCATC 7 cut(s) 148, 231, 303, 320, 605, 953, 1027
BceAI ACGGC 1 cut(s) 7
BciT130I CCWGG 2 cut(s) 186, 416
BcoDI GTCTC 1 cut(s) 220
BfoI RGCGCY 2 cut(s) 295, 1012
BglII AGATCT 1 cut(s) 664
BisI GCNGC 3 cut(s) 306, 442, 579
BlsI GCNGC 3 cut(s) 307, 443, 580
BmcAI AGTACT 1 cut(s) 612
Bme1390I CCNGG 2 cut(s) 186, 416
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 3 cut(s) 293, 871, 943
BmrFI CCNGG 2 cut(s) 186, 416
BmsI GCATC 2 cut(s) 291, 812
BplI GAGNNNNNCTC 2 cut(s) 998, 1030
BpmI CTGGAG 1 cut(s) 342
Bsa29I ATCGAT 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 582, 999
BsaXI ACNNNNNCTCC 4 cut(s) 600, 630, 916, 946
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 1 cut(s) 618
BseBI CCWGG 2 cut(s) 186, 416
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 582, 999
BseGI GGATG 2 cut(s) 295, 892
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 2 cut(s) 110, 948
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 1 cut(s) 924
BseSI GKGCMC 3 cut(s) 11, 250, 874
BseXI GCAGC 3 cut(s) 292, 453, 565
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 2 cut(s) 184, 497
BshNI GGYRCC 3 cut(s) 291, 869, 941
BshVI ATCGAT 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 490
BsiSI CCGG 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 220
BsmBI CGTCTC 1 cut(s) 220
BsmI GAATGC 1 cut(s) 971
BsnI GGCC 2 cut(s) 184, 497
Bsp1286I GDGCHC 4 cut(s) 11, 250, 490, 874
Bsp143I GATC 2 cut(s) 353, 664
Bsp68I TCGCGA 1 cut(s) 492
BspACI CCGC 3 cut(s) 450, 768, 901
BspANI GGCC 2 cut(s) 184, 497
BspCNI CTCAG 2 cut(s) 109, 949
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 1 cut(s) 492
BspLI GGNNCC 3 cut(s) 293, 871, 943
BspPI GGATC 1 cut(s) 361
BspT107I GGYRCC 3 cut(s) 291, 869, 941
BsrI ACTGG 1 cut(s) 618
BssECI CCNNGG 2 cut(s) 582, 999
BssMI GATC 2 cut(s) 353, 664
BssNI GRCGYC 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 186, 416
Bst4CI ACNGT 6 cut(s) 18, 127, 151, 478, 928, 1000
BstACI GRCGYC 1 cut(s) 292
BstAPI GCANNNNNTGC 1 cut(s) 447
BstC8I GCNNGC 3 cut(s) 490, 516, 589
BstDEI CTNAG 3 cut(s) 96, 708, 957
BstDSI CCRYGG 1 cut(s) 999
BstEII GGTNACC 1 cut(s) 157
BstF5I GGATG 2 cut(s) 295, 892
BstFNI CGCG 1 cut(s) 492
BstH2I RGCGCY 2 cut(s) 295, 1012
BstHHI GCGC 2 cut(s) 294, 1011
BstKTI GATC 2 cut(s) 356, 667
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 2 cut(s) 353, 664
BstMWI GCNNNNNNNGC 6 cut(s) 368, 447, 494, 584, 709, 815
BstNI CCWGG 2 cut(s) 186, 416
BstPI GGTNACC 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 184, 414
BstSLI GKGCMC 3 cut(s) 11, 250, 874
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 292, 453, 565
BstX2I RGATCY 2 cut(s) 353, 664
BstXI CCANNNNNNTGG 1 cut(s) 154
BstYI RGATCY 2 cut(s) 353, 664
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 2 cut(s) 184, 497
BsuTUI ATCGAT 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 999
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 2 cut(s) 295, 892
BtsI GCAGTG 2 cut(s) 276, 277
BtsIMutI CAGTG 5 cut(s) 14, 123, 276, 277, 430
BtuMI TCGCGA 1 cut(s) 492
Cac8I GCNNGC 3 cut(s) 490, 516, 589
CaiI CAGNNNCTG 1 cut(s) 447
CfoI GCGC 2 cut(s) 294, 1011
Cfr13I GGNCC 1 cut(s) 182
ClaI ATCGAT 1 cut(s) 324
CsiI ACCWGGT 1 cut(s) 414
Csp6I GTAC 1 cut(s) 611
CviAII CATG 3 cut(s) 288, 436, 672
CviQI GTAC 1 cut(s) 611
DdeI CTNAG 3 cut(s) 96, 708, 957
DinI GGCGCC 1 cut(s) 293
DpnI GATC 2 cut(s) 355, 666
DpnII GATC 2 cut(s) 353, 664
Eco147I AGGCCT 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 184, 414
EgeI GGCGCC 1 cut(s) 293
EheI GGCGCC 1 cut(s) 293
Esp3I CGTCTC 1 cut(s) 220
FaeI CATG 3 cut(s) 291, 439, 675
FalI AAGNNNNNCTT 2 cut(s) 975, 1007
FatI CATG 3 cut(s) 287, 435, 671
FauI CCCGC 1 cut(s) 761
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 306, 442, 579
FokI GGATG 2 cut(s) 282, 899
Fsp4HI GCNGC 3 cut(s) 306, 442, 579
GlaI GCGC 2 cut(s) 293, 1010
GluI GCNGC 3 cut(s) 306, 442, 579
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 2 cut(s) 295, 1012
HaeIII GGCC 2 cut(s) 184, 497
HapII CCGG 1 cut(s) 135
HhaI GCGC 2 cut(s) 294, 1011
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 3 cut(s) 291, 439, 675
Hin6I GCGC 2 cut(s) 292, 1009
HinP1I GCGC 2 cut(s) 292, 1009
HincII GTYRAC 1 cut(s) 783
HindII GTYRAC 1 cut(s) 783
HinfI GANTC 3 cut(s) 254, 274, 758
HpaII CCGG 1 cut(s) 135
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 466, 783
Hpy188I TCNGA 7 cut(s) 85, 99, 259, 300, 402, 757, 763
Hpy188III TCNNGA 7 cut(s) 351, 410, 471, 491, 655, 853, 911
Hpy8I GTNNAC 2 cut(s) 466, 783
Hpy99I CGWCG 2 cut(s) 170, 173
HpyAV CCTTC 3 cut(s) 173, 758, 908
HpyCH4III ACNGT 6 cut(s) 18, 127, 151, 478, 928, 1000
HpyCH4V TGCA 4 cut(s) 282, 319, 518, 803
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 447, 494, 584, 709, 815
HpyF3I CTNAG 3 cut(s) 96, 708, 957
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 3 cut(s) 291, 439, 675
HspAI GCGC 2 cut(s) 292, 1009
KasI GGCGCC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 353, 664
LmnI GCTCC 1 cut(s) 329
Lsp1109I GCAGC 3 cut(s) 292, 453, 565
LweI GCATC 2 cut(s) 291, 812
MabI ACCWGGT 1 cut(s) 414
MaeIII GTNAC 4 cut(s) 157, 275, 748, 928
MalI GATC 2 cut(s) 355, 666
MboI GATC 2 cut(s) 353, 664
MboII GAAGA 1 cut(s) 899
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 2 cut(s) 353, 664
MhlI GDGCHC 4 cut(s) 11, 250, 490, 874
MluCI AATT 7 cut(s) 79, 174, 405, 573, 620, 793, 813
Mly113I GGCGCC 1 cut(s) 292
MlyI GAGTC 3 cut(s) 248, 283, 752
MmeI TCCRAC 3 cut(s) 425, 523, 1019
MseI TTAA 3 cut(s) 561, 623, 980
MslI CAYNNNNRTG 2 cut(s) 152, 324
MspA1I CMGCKG 3 cut(s) 362, 809, 836
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 2 cut(s) 186, 416
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 971
MvaI CCWGG 2 cut(s) 186, 416
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 6 cut(s) 368, 447, 494, 584, 709, 815
NarI GGCGCC 1 cut(s) 292
NdeI CATATG 1 cut(s) 532
NdeII GATC 2 cut(s) 353, 664
NlaIII CATG 3 cut(s) 291, 439, 675
NlaIV GGNNCC 3 cut(s) 293, 871, 943
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 3 cut(s) 157, 275, 928
NruI TCGCGA 1 cut(s) 492
PceI AGGCCT 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 1 cut(s) 971
PkrI GCNGC 3 cut(s) 307, 443, 580
PleI GAGTC 3 cut(s) 248, 282, 752
PluTI GGCGCC 1 cut(s) 295
PpsI GAGTC 3 cut(s) 248, 282, 752
PshBI ATTAAT 1 cut(s) 561
PsiI TTATAA 1 cut(s) 1030
Psp6I CCWGG 2 cut(s) 184, 414
PspEI GGTNACC 1 cut(s) 157
PspGI CCWGG 2 cut(s) 184, 414
PspN4I GGNNCC 3 cut(s) 293, 871, 943
PspPI GGNCC 1 cut(s) 182
PstNI CAGNNNCTG 1 cut(s) 447
PsuI RGATCY 2 cut(s) 353, 664
PvuII CAGCTG 3 cut(s) 362, 809, 836
RruI TCGCGA 1 cut(s) 492
RsaI GTAC 1 cut(s) 612
RsaNI GTAC 1 cut(s) 611
RseI CAYNNNNRTG 2 cut(s) 152, 324
SaqAI TTAA 3 cut(s) 561, 623, 980
SatI GCNGC 3 cut(s) 306, 442, 579
Sau3AI GATC 2 cut(s) 353, 664
Sau96I GGNCC 1 cut(s) 182
ScaI AGTACT 1 cut(s) 612
SchI GAGTC 3 cut(s) 248, 283, 752
ScrFI CCNGG 2 cut(s) 186, 416
SduI GDGCHC 4 cut(s) 11, 250, 490, 874
SexAI ACCWGGT 1 cut(s) 414
SfaNI GCATC 2 cut(s) 291, 812
SfoI GGCGCC 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 152, 324
Sse9I AATT 7 cut(s) 79, 174, 405, 573, 620, 793, 813
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 3 cut(s) 450, 768, 901
SspDI GGCGCC 1 cut(s) 291
SspI AATATT 1 cut(s) 559
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 184, 414
TaaI ACNGT 6 cut(s) 18, 127, 151, 478, 928, 1000
TaqI TCGA 2 cut(s) 324, 797
TasI AATT 7 cut(s) 79, 174, 405, 573, 620, 793, 813
TatI WGTACW 1 cut(s) 610
Tru1I TTAA 3 cut(s) 561, 623, 980
Tru9I TTAA 3 cut(s) 561, 623, 980
TscAI CASTG 5 cut(s) 21, 130, 276, 284, 430
TseFI GTSAC 3 cut(s) 157, 275, 928
TseI GCWGC 3 cut(s) 305, 441, 578
Tsp45I GTSAC 3 cut(s) 157, 275, 928
TspDTI ATGAA 1 cut(s) 16
TspGWI ACGGA 2 cut(s) 24, 186
TspRI CASTG 5 cut(s) 21, 130, 276, 284, 430
VspI ATTAAT 1 cut(s) 561
XapI RAATTY 2 cut(s) 79, 793
ZrmI AGTACT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.