MD16G1192900.v1.1

5'-nucleotidase surE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006194
Physical Location & Seq
Reverse (-)
17099736 .. 17111472
11737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1192900.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGAAAACACCACTGTCATCTCCGGCGGCAAGCCCACCGTGATGGTCACAAACGACGACGGAATCGAAGGCCCTGGCATTCTCGCTCTCGTCCAAGTCCTCGTATCCGCCCATCGCTACAATGTCCTCGTCTGTGCCCCCGACTCGGAGAAGTCGGCAGTGAGCCACTGCATCACATGGCTCCATCCGATTGCTGCCAAGAAAGTGCATATCAATGGAGCAACAGTGTATGCTGTTTCTGGATCTCCAGCTGATTGTGCTTCTTTGGGTGTTTCCAAAACGCTTTTTCCTTCAATTCCTGACCTGGTAATCAGTGGCATAAACCAGGGCAGCAACTGCGGTTATCACATTGTTTACTCTGGAACGGTGGCTGGTGCCCGTGAGGCCTTTCTTAACGGTGTACCTGCTATCTCTATATCATATGATTGGGTTAGAGGTAAGAGCAATATTAATGACTTCACACTTGCTGCCGAGGCTTGCTTACCGATCATAAATGGAGTACTGGTGGAATTAAGAAACAAAACCTATCCTCAAAGATGTTTCTTGAATGTAGATCTTCCATGTAATGTTGCTAATCATAAGGGGTATAAGCTGACTAAGCAAGGCAAAAGTATAATAAAAATGGGGTGGAGGCAAGTTACTTCTGACACTGAAGGCGGAAAAATGTTGTCAACAATGGTAAATTCAACTGCATCAGCTGAAAGTGATACATCAACCACACCAGCTGAAAATCTTTATTTCGCGAGAGAAGTAAGGGGTGCCCAAGTTGATGAGGATGATAATGACCACCGCTTTCTTCAAGAAGGATATATTACTGTCACTCCTCTTGGTGCCATAACAAATGCTGAGATAGACTGCCACACCTACTTGAAAGAGTGGCTTCCAAAAGTGGTTGAGCGCTCCTCTCCATCAGCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
SurE

Protein Analysis

307

Amino Acids

32.86

Weight (kDa)

5.9

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SurE PF01975 14 - 205 4e-54 Survival protein SurE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 919
Acc36I ACCTGC 1 cut(s) 412
AccB1I GGYRCC 3 cut(s) 374, 758, 830
AccII CGCG 1 cut(s) 743
AciI CCGC 5 cut(s) 27, 108, 339, 657, 790
AclWI GGATC 1 cut(s) 250
AcsI RAATTY 1 cut(s) 682
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 2 cut(s) 402, 501
AfeI AGCGCT 1 cut(s) 899
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 5 cut(s) 294, 547, 687, 800, 871
AjnI CCWGG 3 cut(s) 73, 303, 324
AluBI AGCT 5 cut(s) 251, 592, 698, 725, 914
AluI AGCT 5 cut(s) 251, 592, 698, 725, 914
AlwI GGATC 1 cut(s) 250
AlwNI CAGNNNCTG 1 cut(s) 336
Aor51HI AGCGCT 1 cut(s) 899
AoxI GGCC 2 cut(s) 70, 384
ApeKI GCWGC 3 cut(s) 194, 330, 467
ApoI RAATTY 1 cut(s) 682
AseI ATTAAT 1 cut(s) 450
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 1 cut(s) 71
BaeGI GKGCMC 3 cut(s) 139, 379, 763
BanI GGYRCC 3 cut(s) 374, 758, 830
BbvI GCAGC 3 cut(s) 181, 342, 454
BccI CCATC 4 cut(s) 37, 120, 192, 916
BciT130I CCWGG 3 cut(s) 75, 305, 326
BciVI GTATCC 1 cut(s) 115
BfoI RGCGCY 1 cut(s) 901
BfuAI ACCTGC 1 cut(s) 412
BfuI GTATCC 1 cut(s) 115
BglI GCCNNNNNGGC 1 cut(s) 383
BglII AGATCT 1 cut(s) 553
BisI GCNGC 4 cut(s) 28, 195, 331, 468
BlsI GCNGC 4 cut(s) 29, 196, 332, 469
BmcAI AGTACT 1 cut(s) 501
Bme1390I CCNGG 3 cut(s) 75, 305, 326
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 4 cut(s) 182, 376, 760, 832
BmrFI CCNGG 3 cut(s) 75, 305, 326
BmsI GCATC 2 cut(s) 180, 701
BplI GAGNNNNNCTC 2 cut(s) 887, 919
BpmI CTGGAG 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 73, 325, 471
BsaXI ACNNNNNCTCC 6 cut(s) 210, 240, 489, 519, 805, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 507
BseBI CCWGG 3 cut(s) 75, 305, 326
BseDI CCNNGG 3 cut(s) 73, 325, 471
BseGI GGATG 2 cut(s) 184, 781
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 1 cut(s) 507
BseRI GAGGAG 2 cut(s) 813, 892
BseSI GKGCMC 3 cut(s) 139, 379, 763
BseXI GCAGC 3 cut(s) 181, 342, 454
Bsh1236I CGCG 1 cut(s) 743
BshFI GGCC 2 cut(s) 72, 386
BshNI GGYRCC 3 cut(s) 374, 758, 830
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 145
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 2 cut(s) 72, 386
Bsp1286I GDGCHC 3 cut(s) 139, 379, 763
Bsp143I GATC 3 cut(s) 242, 486, 553
Bsp68I TCGCGA 1 cut(s) 743
BspACI CCGC 5 cut(s) 27, 108, 339, 657, 790
BspANI GGCC 2 cut(s) 72, 386
BspCNI CTCAG 1 cut(s) 838
BspFNI CGCG 1 cut(s) 743
BspLI GGNNCC 4 cut(s) 182, 376, 760, 832
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 1 cut(s) 250
BspT107I GGYRCC 3 cut(s) 374, 758, 830
BsrI ACTGG 1 cut(s) 507
BssECI CCNNGG 3 cut(s) 73, 325, 471
BssMI GATC 3 cut(s) 242, 486, 553
Bst2UI CCWGG 3 cut(s) 75, 305, 326
Bst4CI ACNGT 6 cut(s) 16, 40, 226, 367, 398, 817
BstAPI GCANNNNNTGC 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 32, 478
BstDEI CTNAG 2 cut(s) 597, 846
BstF5I GGATG 2 cut(s) 184, 781
BstFNI CGCG 1 cut(s) 743
BstH2I RGCGCY 1 cut(s) 901
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 3 cut(s) 245, 489, 556
BstMBI GATC 3 cut(s) 242, 486, 553
BstMWI GCNNNNNNNGC 5 cut(s) 257, 336, 383, 473, 598
BstNI CCWGG 3 cut(s) 75, 305, 326
BstSCI CCNGG 3 cut(s) 73, 303, 324
BstSLI GKGCMC 3 cut(s) 139, 379, 763
BstUI CGCG 1 cut(s) 743
BstV1I GCAGC 3 cut(s) 181, 342, 454
BstX2I RGATCY 2 cut(s) 242, 553
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 2 cut(s) 242, 553
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 2 cut(s) 72, 386
BtgZI GCGATG 1 cut(s) 98
BtsCI GGATG 2 cut(s) 184, 781
BtsI GCAGTG 2 cut(s) 165, 166
BtsIMutI CAGTG 6 cut(s) 12, 165, 166, 231, 319, 648
BtuMI TCGCGA 1 cut(s) 743
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 32, 478
CaiI CAGNNNCTG 1 cut(s) 336
CfoI GCGC 1 cut(s) 900
Cfr13I GGNCC 1 cut(s) 71
CsiI ACCWGGT 1 cut(s) 303
Csp6I GTAC 2 cut(s) 401, 500
CviAII CATG 2 cut(s) 177, 561
CviQI GTAC 2 cut(s) 401, 500
DdeI CTNAG 2 cut(s) 597, 846
DpnI GATC 3 cut(s) 244, 488, 555
DpnII GATC 3 cut(s) 242, 486, 553
EciI GGCGGA 2 cut(s) 97, 672
Eco147I AGGCCT 1 cut(s) 386
Eco47III AGCGCT 1 cut(s) 899
Eco57I CTGAAG 1 cut(s) 672
EcoO109I RGGNCCY 1 cut(s) 71
EcoRII CCWGG 3 cut(s) 73, 303, 324
FaeI CATG 2 cut(s) 180, 564
FalI AAGNNNNNCTT 2 cut(s) 864, 896
FatI CATG 2 cut(s) 176, 560
FauNDI CATATG 1 cut(s) 421
Fnu4HI GCNGC 4 cut(s) 28, 195, 331, 468
FokI GGATG 2 cut(s) 171, 788
Fsp4HI GCNGC 4 cut(s) 28, 195, 331, 468
GlaI GCGC 1 cut(s) 899
GluI GCNGC 4 cut(s) 28, 195, 331, 468
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 901
HaeIII GGCC 2 cut(s) 72, 386
HapII CCGG 1 cut(s) 24
HhaI GCGC 1 cut(s) 900
Hin1II CATG 2 cut(s) 180, 564
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 1 cut(s) 672
HindII GTYRAC 1 cut(s) 672
HinfI GANTC 2 cut(s) 63, 143
HpaII CCGG 1 cut(s) 24
Hpy166II GTNNAC 3 cut(s) 355, 401, 672
Hpy188I TCNGA 3 cut(s) 148, 189, 646
Hpy188III TCNNGA 6 cut(s) 240, 299, 360, 544, 742, 800
Hpy8I GTNNAC 3 cut(s) 355, 401, 672
Hpy99I CGWCG 2 cut(s) 59, 62
HpyAV CCTTC 4 cut(s) 62, 300, 647, 797
HpyCH4III ACNGT 6 cut(s) 16, 40, 226, 367, 398, 817
HpyCH4V TGCA 3 cut(s) 171, 208, 692
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 336, 383, 473, 598
HpyF3I CTNAG 2 cut(s) 597, 846
Hsp92II CATG 2 cut(s) 180, 564
HspAI GCGC 1 cut(s) 898
Kzo9I GATC 3 cut(s) 242, 486, 553
LmnI GCTCC 3 cut(s) 186, 218, 905
Lsp1109I GCAGC 3 cut(s) 181, 342, 454
LweI GCATC 2 cut(s) 180, 701
MabI ACCWGGT 1 cut(s) 303
MaeIII GTNAC 3 cut(s) 46, 637, 817
MalI GATC 3 cut(s) 244, 488, 555
MboI GATC 3 cut(s) 242, 486, 553
MboII GAAGA 2 cut(s) 548, 788
MflI RGATCY 2 cut(s) 242, 553
MhlI GDGCHC 3 cut(s) 139, 379, 763
MluCI AATT 3 cut(s) 294, 509, 682
MlyI GAGTC 1 cut(s) 137
MseI TTAA 3 cut(s) 393, 450, 512
MslI CAYNNNNRTG 2 cut(s) 41, 213
MspA1I CMGCKG 3 cut(s) 251, 698, 725
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 3 cut(s) 75, 305, 326
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 3 cut(s) 75, 305, 326
MvnI CGCG 1 cut(s) 743
MwoI GCNNNNNNNGC 5 cut(s) 257, 336, 383, 473, 598
NdeI CATATG 1 cut(s) 421
NdeII GATC 3 cut(s) 242, 486, 553
NlaIII CATG 2 cut(s) 180, 564
NlaIV GGNNCC 4 cut(s) 182, 376, 760, 832
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 2 cut(s) 46, 817
NruI TCGCGA 1 cut(s) 743
PceI AGGCCT 1 cut(s) 386
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 1 cut(s) 63
PkrI GCNGC 4 cut(s) 29, 196, 332, 469
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PshBI ATTAAT 1 cut(s) 450
PsiI TTATAA 1 cut(s) 919
Psp6I CCWGG 3 cut(s) 73, 303, 324
PspGI CCWGG 3 cut(s) 73, 303, 324
PspN4I GGNNCC 4 cut(s) 182, 376, 760, 832
PspPI GGNCC 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 336
PsuI RGATCY 2 cut(s) 242, 553
PvuII CAGCTG 3 cut(s) 251, 698, 725
RruI TCGCGA 1 cut(s) 743
RsaI GTAC 2 cut(s) 402, 501
RsaNI GTAC 2 cut(s) 401, 500
RseI CAYNNNNRTG 2 cut(s) 41, 213
SaqAI TTAA 3 cut(s) 393, 450, 512
SatI GCNGC 4 cut(s) 28, 195, 331, 468
Sau3AI GATC 3 cut(s) 242, 486, 553
Sau96I GGNCC 1 cut(s) 71
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 3 cut(s) 75, 305, 326
SduI GDGCHC 3 cut(s) 139, 379, 763
SexAI ACCWGGT 1 cut(s) 303
SfaNI GCATC 2 cut(s) 180, 701
SmiMI CAYNNNNRTG 2 cut(s) 41, 213
Sse9I AATT 3 cut(s) 294, 509, 682
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 5 cut(s) 27, 108, 339, 657, 790
SspI AATATT 1 cut(s) 448
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 3 cut(s) 73, 303, 324
TaaI ACNGT 6 cut(s) 16, 40, 226, 367, 398, 817
TaqI TCGA 1 cut(s) 66
TasI AATT 3 cut(s) 294, 509, 682
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 1 cut(s) 63
Tru1I TTAA 3 cut(s) 393, 450, 512
Tru9I TTAA 3 cut(s) 393, 450, 512
TscAI CASTG 6 cut(s) 19, 165, 173, 231, 319, 655
TseFI GTSAC 2 cut(s) 46, 817
TseI GCWGC 3 cut(s) 194, 330, 467
Tsp45I GTSAC 2 cut(s) 46, 817
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 6 cut(s) 19, 165, 173, 231, 319, 655
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 1 cut(s) 682
ZrmI AGTACT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.