Rroxscaffold_2G00115680

5'-nucleotidase surE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006194
Physical Location & Seq
Forward (+)
42072434 .. 42078590
6157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115680.1

Sequence Viewer

Length: 738 bp
ATGTCGGTCAACCCATCGTCGACCGCTTGCTCTCCGATTACTTTGAGTGGACCACCAAGGACTACCGCTCCATGGACCTTTGGTGATCTCGTCGTGGAGCATCTCGCAGTTCCGGTCGACGGCCAGTCCTTTAGAGATCTGCCAGGGTTCTGTGGGAAGGACAGCGGCGGCACTCATCTTTTGGGTTGGTGGCTGCTTATGGAGATTCTTCATTATCATACTTTCTCGGGCAGTGGCCGGTCAATAAATCAAAGGCCACTAGGCCAACAATGGGTTTACTTTCCTAAAGCTATTATTCTCTCGGTCTGCTCACTTTGCAATCTCTTCCTCTCTCTTTCTCATTCCTTTCTCGCAATCTCTCTCTCTCTGCACCTCCAACAGTACAAGACTGCAAGCTTACCACAATTCCAATGGAAGTTGTTCACGTCCTCGTCTCCACCAATCGCTACGGTGTTCTCGTCGCGCTCCCCGACTCGGAAAACTCGCTTCGTGAGTCACCGCATCACTTGGCGTCGTCCGATTGCCGCCAAACAAGTTGACATCGCCGCAGCAACGGCTTTTGCTGTTTACGGGTCTCCAGCTGATTGTGCTTCTTTGGGCGTTTCTAAAAGCGTTTTTTTTCCTTCGGTTCCCGACTTGGTGATCGGCGGCATAAACAAGGGCAGCAACCGTGGTCACCACATTGTTTACTCGATACGCCAACAAGCGTTGCTACTCATAAGGGGTATAAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
SurE

Protein Analysis

245

Amino Acids

26.75

Weight (kDa)

9.87

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SurE PF01975 157 - 231 2.9e-14 Survival protein SurE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 68
AccI GTMKAC 2 cut(s) 20, 117
AccII CGCG 1 cut(s) 463
AciI CCGC 8 cut(s) 24, 66, 165, 168, 499, 525, 546, 648
AcoI YGGCCR 2 cut(s) 121, 235
AcyI GRCGYC 1 cut(s) 511
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 4 cut(s) 72, 119, 271, 474
AhdI GACNNNNNGTC 1 cut(s) 124
AjiI CACGTC 1 cut(s) 426
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 3 cut(s) 290, 396, 581
AluI AGCT 3 cut(s) 290, 396, 581
Alw26I GTCTC 2 cut(s) 438, 579
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 4 cut(s) 121, 235, 254, 262
ApeKI GCWGC 3 cut(s) 193, 548, 663
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 2 cut(s) 50, 75
AsuHPI GGTGA 4 cut(s) 95, 488, 652, 668
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 2 cut(s) 50, 75
BbvI GCAGC 3 cut(s) 180, 560, 675
BccI CCATC 1 cut(s) 22
BceAI ACGGC 2 cut(s) 136, 570
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 438, 579
BfaI CTAG 1 cut(s) 260
BglII AGATCT 1 cut(s) 136
BisI GCNGC 8 cut(s) 166, 169, 194, 525, 546, 549, 649, 664
BlsI GCNGC 8 cut(s) 167, 170, 195, 526, 547, 550, 650, 665
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 2 cut(s) 50, 75
BmeRI GACNNNNNGTC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 426
BmgT120I GGNCC 2 cut(s) 50, 75
BmiI GGNNCC 1 cut(s) 630
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 2 cut(s) 109, 510
BpmI CTGGAG 1 cut(s) 561
BsaHI GRCGYC 1 cut(s) 511
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 4 cut(s) 56, 71, 143, 670
BsaWI WCCGGW 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 119, 271, 474
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 4 cut(s) 56, 71, 143, 670
BseLI CCNNNNNNNGG 4 cut(s) 72, 119, 271, 474
BseNI ACTGG 1 cut(s) 124
BseXI GCAGC 3 cut(s) 180, 560, 675
BsgI GTGCAG 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 463
Bsh1285I CGRYCG 2 cut(s) 24, 117
BshFI GGCC 4 cut(s) 123, 237, 256, 264
BsiEI CGRYCG 2 cut(s) 24, 117
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 2 cut(s) 113, 238
BslI CCNNNNNNNGG 4 cut(s) 72, 119, 271, 474
BsmAI GTCTC 2 cut(s) 438, 579
BsmBI CGTCTC 1 cut(s) 438
BsnI GGCC 4 cut(s) 123, 237, 256, 264
Bso31I GGTCTC 1 cut(s) 579
BsoBI CYCGRG 1 cut(s) 226
Bsp143I GATC 3 cut(s) 85, 136, 642
Bsp19I CCATGG 1 cut(s) 71
BspACI CCGC 8 cut(s) 24, 66, 165, 168, 499, 525, 546, 648
BspANI GGCC 4 cut(s) 123, 237, 256, 264
BspFNI CGCG 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 630
BspTNI GGTCTC 1 cut(s) 579
BsrBI CCGCTC 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 4 cut(s) 56, 71, 143, 670
BssMI GATC 3 cut(s) 85, 136, 642
BssNI GRCGYC 1 cut(s) 511
BssT1I CCWWGG 2 cut(s) 56, 71
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 3 cut(s) 381, 451, 671
Bst6I CTCTTC 1 cut(s) 329
BstACI GRCGYC 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 28, 394
BstDSI CCRYGG 2 cut(s) 71, 670
BstEII GGTNACC 1 cut(s) 674
BstFNI CGCG 1 cut(s) 463
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 3 cut(s) 88, 139, 645
BstMAI GTCTC 2 cut(s) 438, 579
BstMBI GATC 3 cut(s) 85, 136, 642
BstMCI CGRYCG 2 cut(s) 24, 117
BstMWI GCNNNNNNNGC 3 cut(s) 315, 554, 587
BstNI CCWGG 1 cut(s) 144
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 1 cut(s) 463
BstV1I GCAGC 3 cut(s) 180, 560, 675
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 4 cut(s) 123, 237, 256, 264
BtgI CCRYGG 2 cut(s) 71, 670
BtgZI GCGATG 1 cut(s) 526
BtrI CACGTC 1 cut(s) 426
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 2 cut(s) 28, 394
CfoI GCGC 1 cut(s) 465
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 50, 75
CseI GACGC 1 cut(s) 500
Csp6I GTAC 1 cut(s) 382
CviAII CATG 1 cut(s) 72
CviJI RGCY 9 cut(s) 123, 193, 237, 256, 264, 290, 396, 557, 581
CviKI_1 RGCY 9 cut(s) 123, 193, 237, 256, 264, 290, 396, 557, 581
CviQI GTAC 1 cut(s) 382
DpnI GATC 3 cut(s) 87, 138, 644
DpnII GATC 3 cut(s) 85, 136, 642
DriI GACNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 2 cut(s) 121, 235
Eam1104I CTCTTC 1 cut(s) 329
Eam1105I GACNNNNNGTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 56, 71
Eco31I GGTCTC 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 50, 75
Eco88I CYCGRG 1 cut(s) 226
Eco91I GGTNACC 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 2 cut(s) 56, 71
ErhI CCWWGG 2 cut(s) 56, 71
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 1 cut(s) 75
FaiI YATR 6 cut(s) 73, 200, 219, 653, 719, 728
FatI CATG 1 cut(s) 71
FblI GTMKAC 2 cut(s) 20, 117
Fnu4HI GCNGC 8 cut(s) 166, 169, 194, 525, 546, 549, 649, 664
Fsp4HI GCNGC 8 cut(s) 166, 169, 194, 525, 546, 549, 649, 664
FspBI CTAG 1 cut(s) 260
GlaI GCGC 1 cut(s) 464
GluI GCNGC 8 cut(s) 166, 169, 194, 525, 546, 549, 649, 664
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 4 cut(s) 123, 237, 256, 264
HapII CCGG 2 cut(s) 113, 238
HgaI GACGC 1 cut(s) 500
HhaI GCGC 1 cut(s) 465
Hin1I GRCGYC 1 cut(s) 511
Hin1II CATG 1 cut(s) 75
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HincII GTYRAC 4 cut(s) 10, 21, 118, 538
HindII GTYRAC 4 cut(s) 10, 21, 118, 538
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 3 cut(s) 205, 472, 493
HpaII CCGG 2 cut(s) 113, 238
HphI GGTGA 4 cut(s) 95, 488, 652, 668
Hpy166II GTNNAC 9 cut(s) 10, 21, 50, 118, 277, 423, 538, 568, 688
Hpy188I TCNGA 3 cut(s) 36, 477, 519
Hpy188III TCNNGA 2 cut(s) 490, 632
Hpy8I GTNNAC 9 cut(s) 10, 21, 50, 118, 277, 423, 538, 568, 688
Hpy99I CGWCG 5 cut(s) 22, 95, 122, 463, 516
HpyAV CCTTC 2 cut(s) 151, 633
HpyCH4III ACNGT 3 cut(s) 381, 451, 671
HpyCH4IV ACGT 1 cut(s) 425
HpyCH4V TGCA 3 cut(s) 318, 370, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 315, 554, 587
HpySE526I ACGT 1 cut(s) 425
Hsp92I GRCGYC 1 cut(s) 511
Hsp92II CATG 1 cut(s) 75
HspAI GCGC 1 cut(s) 463
Kzo9I GATC 3 cut(s) 85, 136, 642
LmnI GCTCC 3 cut(s) 73, 97, 470
LpnPI CCDG 6 cut(s) 126, 129, 137, 156, 251, 591
Lsp1109I GCAGC 3 cut(s) 180, 560, 675
LweI GCATC 2 cut(s) 109, 510
MaeI CTAG 1 cut(s) 260
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 3 cut(s) 494, 674, 731
MalI GATC 3 cut(s) 87, 138, 644
MbiI CCGCTC 1 cut(s) 68
MboI GATC 3 cut(s) 85, 136, 642
MboII GAAGA 2 cut(s) 200, 316
MflI RGATCY 1 cut(s) 136
MluCI AATT 1 cut(s) 404
MlyI GAGTC 2 cut(s) 466, 502
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 3 cut(s) 338, 383, 439
MroXI GAANNNNTTC 1 cut(s) 419
MspA1I CMGCKG 2 cut(s) 165, 581
MspI CCGG 2 cut(s) 113, 238
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 3 cut(s) 315, 554, 587
NcoI CCATGG 1 cut(s) 71
NdeII GATC 3 cut(s) 85, 136, 642
NlaIII CATG 1 cut(s) 75
NlaIV GGNNCC 1 cut(s) 630
NmuCI GTSAC 3 cut(s) 494, 674, 731
PcsI WCGNNNNNNNCGW 2 cut(s) 455, 467
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 1 cut(s) 205
PkrI GCNGC 8 cut(s) 167, 170, 195, 526, 547, 550, 650, 665
PleI GAGTC 2 cut(s) 466, 501
PpsI GAGTC 2 cut(s) 466, 501
Psp6I CCWGG 1 cut(s) 142
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 630
PspPI GGNCC 2 cut(s) 50, 75
PsuI RGATCY 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SalI GTCGAC 2 cut(s) 19, 116
SatI GCNGC 8 cut(s) 166, 169, 194, 525, 546, 549, 649, 664
Sau3AI GATC 3 cut(s) 85, 136, 642
Sau96I GGNCC 2 cut(s) 50, 75
SchI GAGTC 2 cut(s) 466, 502
ScrFI CCNGG 1 cut(s) 144
SetI ASST 6 cut(s) 80, 292, 375, 398, 428, 583
SfaNI GCATC 2 cut(s) 109, 510
SinI GGWCC 2 cut(s) 50, 75
Sse9I AATT 1 cut(s) 404
SsiI CCGC 8 cut(s) 24, 66, 165, 168, 499, 525, 546, 648
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 2 cut(s) 56, 71
TaaI ACNGT 3 cut(s) 381, 451, 671
TaiI ACGT 1 cut(s) 428
TaqI TCGA 3 cut(s) 20, 117, 692
TaqII GACCGA 1 cut(s) 292
TasI AATT 1 cut(s) 404
TatI WGTACW 1 cut(s) 381
TauI GCSGC 5 cut(s) 168, 171, 527, 548, 651
TfiI GAWTC 1 cut(s) 205
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 3 cut(s) 494, 674, 731
TseI GCWGC 3 cut(s) 193, 548, 663
Tsp45I GTSAC 3 cut(s) 494, 674, 731
TspDTI ATGAA 1 cut(s) 200
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 50, 75
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XmiI GTMKAC 2 cut(s) 20, 117
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.