Rorug04G0141000

5'-nucleotidase surE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006194
Physical Location & Seq
Reverse (-)
23302246 .. 23304711
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0141000.1

Sequence Viewer

Length: 2466 bp
ATGGAGCTGAGAAGGGTTTCAATCTCGAAACCTTGCTCTTTCTTAATCAAACGCCCATTAAGCTGCGTCCCACGTCCTCAGCCTCCGGCGCCGCCGCCGCCACCGAATAACCAGAGATTACTTGACCGGGTATCCTCTGTTCTCTCAAACTCATCTTTCAAGTACCCTCAATGTAGATCTCTCATATCCCTCTTGTCCCCTCATGAATTCGATGCCCTCTTCTATTCCGTCGCCACTTCCCCCTCCTCCAATGTCAACCCCAAAACTGCTCTCCGTTTCTTTCACTTCGCTTCCAAAGCTTTCAACTTCCATTTTACCCTCGCCTCTTATTGCCTTTTGGTTCGTGTACTAATTCTTTCCAATCTTAACTCGCCTGCGAGATTGCTTTTGATCCGCTTGATTGATGGCAATGTGCAAGCTTTGTATGCTCTCCCGCCCGCTGATAGGCACATTGAGATAGCCATGGCTATGTCCCACTTCAAAACCGCCTCCGAAAGATCTGCTGTGCTTCAGGCGTTGGATATGTTGATTCATCTTTACTGTACTCAGTTCAAGGATTTGGGTTTTTGTTCTGCCGTTCATGTTTTTGAGTTTTTCTCTAATAAGGCTATGTTTCCGTCCCTCAAGACTTGCCATTTTTTGTTGAATTCTTTAGTCAAGGCGGACCAACTTGATAAGAGCTACCGTGTGTTTGAAGTTCTGTCTCGTGGTGTTTCTCCGGATGTTTACTTGTTTACTACTGCAATTCAAGCATTTTGTAAGGGAGGGAAGGTTGATGAGGCATTAGGTTTGTTCTCTAAAATGGAGGCACTTGGCACTGCTCCAAATGTTGTTACTTACAACAATGTTATTCACGGGTTATGTAAGAGCAGAAGATTAGAGGAGGCTTTCCAATTCAAGGACAAGATGGTACAAAACAATGTGAATCCCAGTCTGACAACATATGGTGTGCTCCTTAATGGTTTGATTAAGTTGGACAAGTTTGACGACGCAAATCATGTTTTGAAGGAAATGTATAGCAAGGGATTTGTCCCCAATGAGGTTATCTATAACATATTGATTGACGGCTATTGTAAAACGGGAAGTACTAGTGAGGCACTGAAGTTAAGGGATGAAATGTTATACAATGGGGTAACTCCAAATTCTGTTACCATTAACTCCCTACTGCAGGGATTTTGTAAAAGCAACCACTTTGAGCATGCTGAGCAGGTCTTAGATAAGGTGCTATCCAGTGGTTCATCTGTAGACCAAGTCGTTTGTTTCTCAGTCATTCACTCGTTATGCAAGAACTCTAGGTTTGATTCTGCACTGAAATTCACAATTGAAATGCTATTAAGAAACTTCAGGCCCAGCGATAACTTGCTTACAACATTGGTTGTTGGGCTCTGTAAAGATGGAAAGCATTCTGAGGCATCTCAGCTTTGGTTTAGGCTATGGGAGAAAGGGTTTCCAGCCAACACAGTGACCTCAAATGCTCTAATTTATGGAGTTTGTGAATCTGGTAGCATGCAAGAGGTTGCTAGGCTACTCAAGGAAATGGTAAAGAGAGGCTTGGTATTGGATAGGATCTCATGCAACACACTCATCTTGGGTTGGTGCAAGGAGGGAAAAGTGGACGAAGCTTTTAAGATTAGGGACTGGATGGGTAAGAAAGGAATTTTGCCAGACACATATACTTACAATTTGCTAATGCATGGTCTATGTAATATGGGAAAAGTGGATGATGCTGTTAAACTTTGGGAGGAGTGCAGAAAACATGGTCTTGCCGATGTCTATACATATGGGGTGATGATAGATGGGTACTGTAGAGCTGACAGAATTAAAGACGGTGAAGACCTATTTATTGAGTTGGTGACTAAGAAACTTCAGCTGAATTTCATTGTTTATAATACGCTTATAAGAGCATACTGTACAAATGCGAACATGATTGGAGCCCTTGGTCTCTGCTGCGACATGAAAAAGAAGGGTATTGAACCAACTTGTACCACATATTCTTCTCTTATACATGGGATGTGCAATATTGGTAATGTCGGAGATGCAGAATACCTTCTTGATCAAATGAGGAAGGAGGGTTTGTTGCCCGATGTTGTTTGCTACACTGCACTAATTCATGGTTATTGTAAGCTAGGCCAGATGGATAAAGTAGGGAGTGTTCTTGGAGAGATGTCTTCATATAACGTACAACCTAATAAGATTACATACACTGTCATGATTGATGGGCATTGTAAACTAGGTAATATGGAAGAAGCAACTAAACTTCTATGCGAGATGGAAAAAAAGAGAATTGTCCCGGATGCTGTCACTTACAATGCCTTAACAAATGGATTTTGCAAGGAAAGGATGGTGGAAGAAGCTTTTGAAGTTTGTGATCAAATGTTCAGTAAAGGTCTAGCTTTAGATGAAATTACCTATACGACATTGGTTAGTGGGCTGCATCAAACAGCTACATGTGCAGACGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
SurE

Protein Analysis

821

Amino Acids

91.92

Weight (kDa)

7.94

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 181 - 320 1.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 232 - 284 2.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 236 - 269 3.1e-10 PPR repeat
PPR_2 PF13041 240 - 289 9e-15 PPR repeat family
PPR PF01535 244 - 272 8.4e-06 PPR repeat
PPR_3 PF13812 264 - 320 5.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 274 - 304 7.4e-11 PPR repeat
PPR_2 PF13041 275 - 324 2.2e-16 PPR repeat family
PPR PF01535 278 - 308 2.1e-06 PPR repeat
PPR_3 PF13812 300 - 357 1.1e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 331 - 438 2.7e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 334 - 391 1.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 341 - 373 2.3e-11 PPR repeat
PPR_2 PF13041 345 - 394 3.3e-17 PPR repeat family
PPR PF01535 348 - 378 3.8e-08 PPR repeat
PPR_1 PF12854 376 - 407 4.3e-06 PPR repeat
PPR_3 PF13812 508 - 565 2.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 516 - 546 2.9e-06 PPR repeat
PPR_2 PF13041 522 - 569 2.3e-17 PPR repeat family
PPR_1 PF12854 551 - 582 3.3e-11 PPR repeat
PPR PF01535 558 - 588 1.6e-06 PPR repeat
PPR_2 PF13041 558 - 603 3.9e-13 PPR repeat family
PPR_1 PF12854 589 - 616 5.1e-06 PPR repeat
PPR_3 PF13812 613 - 669 3.5e-09 Pentatricopeptide repeat domain
PPR_long PF17177 613 - 730 4e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 619 - 714 5.6e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 627 - 673 7.5e-14 PPR repeat family
PPR_3 PF13812 651 - 706 2.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 655 - 688 1e-10 PPR repeat
PPR_2 PF13041 666 - 703 1.4e-07 PPR repeat family
PPR_3 PF13812 683 - 738 3.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 691 - 714 6.5e-08 PPR repeat
PPR_2 PF13041 694 - 743 1.2e-17 PPR repeat family
PPR PF01535 697 - 726 2.1e-06 PPR repeat
PPR_long PF17177 714 - 808 8.5e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 717 - 774 2.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 726 - 758 7.6e-11 PPR repeat
PPR PF01535 732 - 762 3.6e-09 PPR repeat
PPR_2 PF13041 737 - 778 8.6e-14 PPR repeat family
PPR_1 PF12854 761 - 792 1.4e-10 PPR repeat
PPR_2 PF13041 764 - 812 4.3e-14 PPR repeat family
PPR PF01535 767 - 797 3.2e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1887, 1898
Acc36I ACCTGC 1 cut(s) 1198
AccB1I GGYRCC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 1245
AccIII TCCGGA 1 cut(s) 718
AciI CCGC 8 cut(s) 92, 95, 98, 394, 434, 438, 486, 662
AclWI GGATC 2 cut(s) 385, 1574
AcsI RAATTY 6 cut(s) 206, 646, 1141, 1313, 1656, 1873
AcuI CTGAAG 4 cut(s) 494, 1121, 1327, 1850
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 9 cut(s) 164, 348, 544, 912, 1087, 1802, 1912, 1984, 2181
AfiI CCNNNNNNNGG 4 cut(s) 444, 898, 1039, 1168
AflIII ACRYGT 1 cut(s) 2447
AhlI ACTAGT 1 cut(s) 1088
AjiI CACGTC 1 cut(s) 74
Alw21I GWGCWC 1 cut(s) 954
Alw26I GTCTC 2 cut(s) 708, 1946
AlwI GGATC 2 cut(s) 385, 1574
Aor13HI TCCGGA 1 cut(s) 718
AoxI GGCC 2 cut(s) 1346, 2128
ApeKI GCWGC 3 cut(s) 63, 1947, 2431
ApoI RAATTY 6 cut(s) 206, 646, 1141, 1313, 1656, 1873
Asp700I GAANNNNTTC 3 cut(s) 16, 1402, 2046
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 664, 1347
AsuC2I CCSGG 2 cut(s) 128, 2291
AsuHPI GGTGA 3 cut(s) 1798, 1841, 1864
AvaII GGWCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 88
BanII GRGCYC 2 cut(s) 1386, 1936
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 2 cut(s) 1839, 2160
Bbv12I GWGCWC 1 cut(s) 954
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 50, 1934, 2418
BccI CCATC 9 cut(s) 398, 901, 1388, 1636, 1790, 2128, 2210, 2263, 2335
BceAI ACGGC 2 cut(s) 560, 1081
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciVI GTATCC 1 cut(s) 142
BclI TGATCA 2 cut(s) 2053, 2368
BcnI CCSGG 2 cut(s) 128, 2291
BcoDI GTCTC 2 cut(s) 708, 1946
BcuI ACTAGT 1 cut(s) 1088
BfaI CTAG 6 cut(s) 1089, 1293, 1521, 2126, 2231, 2390
BfmI CTRYAG 3 cut(s) 1166, 1242, 1804
BfoI RGCGCY 1 cut(s) 92
BfuAI ACCTGC 1 cut(s) 1198
BfuI GTATCC 1 cut(s) 142
BglII AGATCT 2 cut(s) 176, 497
BisI GCNGC 6 cut(s) 64, 92, 95, 98, 1948, 2432
BlpI GCTNAGC 1 cut(s) 1203
BlsI GCNGC 6 cut(s) 65, 93, 96, 99, 1949, 2433
BmcAI AGTACT 1 cut(s) 1087
Bme1390I CCNGG 2 cut(s) 128, 2291
Bme18I GGWCC 1 cut(s) 664
BmgBI CACGTC 1 cut(s) 74
BmgT120I GGNCC 2 cut(s) 664, 1347
BmiI GGNNCC 2 cut(s) 90, 1933
BmrFI CCNGG 2 cut(s) 128, 2291
BmrI ACTGGG 1 cut(s) 924
BmsI GCATC 6 cut(s) 202, 1421, 1714, 2026, 2284, 2443
BmuI ACTGGG 1 cut(s) 924
BpiI GAAGAC 2 cut(s) 1839, 2160
BplI GAGNNNNNCTC 2 cut(s) 581, 613
Bpu10I CCTNAGC 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 1203
BpuEI CTTGAG 2 cut(s) 608, 1514
BpuMI CCSGG 2 cut(s) 128, 2291
BsaHI GRCGYC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 1946
BsaJI CCNNGG 2 cut(s) 462, 1936
BsaWI WCCGGW 1 cut(s) 718
BsaXI ACNNNNNCTCC 6 cut(s) 756, 786, 1430, 1460, 2060, 2090
Bsc4I CCNNNNNNNGG 4 cut(s) 444, 898, 1039, 1168
Bse1I ACTGG 3 cut(s) 930, 1230, 1643
Bse3DI GCAATG 1 cut(s) 415
BseAI TCCGGA 1 cut(s) 718
BseDI CCNNGG 2 cut(s) 462, 1936
BseGI GGATG 7 cut(s) 727, 1117, 1647, 1726, 2016, 2299, 2346
BseLI CCNNNNNNNGG 4 cut(s) 444, 898, 1039, 1168
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 6 cut(s) 92, 560, 1194, 1278, 1398, 1430
BseNI ACTGG 3 cut(s) 930, 1230, 1643
BseRI GAGGAG 3 cut(s) 235, 896, 1757
BseXI GCAGC 3 cut(s) 50, 1934, 2418
BseYI CCCAGC 1 cut(s) 1349
BsgI GTGCAG 3 cut(s) 1290, 1768, 2085
BshFI GGCC 2 cut(s) 1348, 2130
BshNI GGYRCC 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 954
BsiSI CCGG 4 cut(s) 86, 127, 719, 2291
BslFI GGGAC 7 cut(s) 53, 181, 457, 604, 1016, 1649, 2273
BslI CCNNNNNNNGG 4 cut(s) 444, 898, 1039, 1168
BsmAI GTCTC 2 cut(s) 708, 1946
BsmFI GGGAC 7 cut(s) 53, 181, 457, 604, 1016, 1649, 2273
BsmI GAATGC 1 cut(s) 1402
BsnI GGCC 2 cut(s) 1348, 2130
Bso31I GGTCTC 1 cut(s) 1946
Bsp1286I GDGCHC 3 cut(s) 954, 1386, 1936
Bsp13I TCCGGA 1 cut(s) 718
Bsp1407I TGTACA 1 cut(s) 1910
Bsp143I GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
Bsp1720I GCTNAGC 1 cut(s) 1203
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 8 cut(s) 92, 95, 98, 394, 434, 438, 486, 662
BspANI GGCC 2 cut(s) 1348, 2130
BspCNI CTCAG 6 cut(s) 91, 559, 1195, 1277, 1399, 1429
BspEI TCCGGA 1 cut(s) 718
BspHI TCATGA 2 cut(s) 202, 2208
BspLI GGNNCC 2 cut(s) 90, 1933
BspMAI CTGCAG 1 cut(s) 1170
BspMI ACCTGC 1 cut(s) 1198
BspPI GGATC 2 cut(s) 385, 1574
BspT107I GGYRCC 1 cut(s) 88
BspTNI GGTCTC 1 cut(s) 1946
BsrDI GCAATG 1 cut(s) 415
BsrGI TGTACA 1 cut(s) 1910
BsrI ACTGG 3 cut(s) 930, 1230, 1643
BssECI CCNNGG 2 cut(s) 462, 1936
BssMI GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
BssNI GRCGYC 1 cut(s) 89
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 2 cut(s) 462, 1936
Bst2BI CACGAG 1 cut(s) 705
Bst4CI ACNGT 7 cut(s) 542, 686, 1462, 1805, 1829, 1910, 2206
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 1910
BstC8I GCNNGC 5 cut(s) 375, 417, 438, 1200, 1508
BstDEI CTNAG 9 cut(s) 8, 78, 546, 1203, 1213, 1264, 1407, 1416, 1857
BstDSI CCRYGG 1 cut(s) 462
BstENI CCTNNNNNAGG 1 cut(s) 1166
BstF5I GGATG 7 cut(s) 727, 1117, 1647, 1726, 2016, 2299, 2346
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 6 cut(s) 179, 393, 500, 1569, 2056, 2371
BstMAI GTCTC 2 cut(s) 708, 1946
BstMBI GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
BstMWI GCNNNNNNNGC 8 cut(s) 60, 88, 97, 296, 425, 749, 1204, 2450
BstNSI RCATGY 3 cut(s) 1202, 1510, 2451
BstSCI CCNGG 2 cut(s) 126, 2289
BstSFI CTRYAG 3 cut(s) 1166, 1242, 1804
BstV1I GCAGC 3 cut(s) 50, 1934, 2418
BstV2I GAAGAC 2 cut(s) 1839, 2160
BstX2I RGATCY 3 cut(s) 176, 497, 1566
BstYI RGATCY 3 cut(s) 176, 497, 1566
BsuI GTATCC 1 cut(s) 142
BsuRI GGCC 2 cut(s) 1348, 2130
BtgI CCRYGG 1 cut(s) 462
BtrI CACGTC 1 cut(s) 74
BtsCI GGATG 7 cut(s) 727, 1117, 1647, 1726, 2016, 2299, 2346
BtsI GCAGTG 2 cut(s) 816, 2097
BtsIMutI CAGTG 7 cut(s) 816, 1097, 1237, 1307, 1467, 2097, 2202
BveI ACCTGC 1 cut(s) 1198
Cac8I GCNNGC 5 cut(s) 375, 417, 438, 1200, 1508
CciI TCATGA 2 cut(s) 202, 2208
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 664, 1347
CseI GACGC 2 cut(s) 55, 998
Csp6I GTAC 9 cut(s) 163, 347, 543, 911, 1086, 1801, 1911, 1983, 2180
CviQI GTAC 9 cut(s) 163, 347, 543, 911, 1086, 1801, 1911, 1983, 2180
DdeI CTNAG 9 cut(s) 8, 78, 546, 1203, 1213, 1264, 1407, 1416, 1857
DinI GGCGCC 1 cut(s) 90
DpnI GATC 6 cut(s) 178, 392, 499, 1568, 2055, 2370
DpnII GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 677
Eco130I CCWWGG 2 cut(s) 462, 1936
Eco24I GRGCYC 2 cut(s) 1386, 1936
Eco31I GGTCTC 1 cut(s) 1946
Eco47I GGWCC 1 cut(s) 664
Eco57I CTGAAG 4 cut(s) 494, 1121, 1327, 1850
EcoNI CCTNNNNNAGG 1 cut(s) 1166
EcoRI GAATTC 2 cut(s) 206, 646
EcoT14I CCWWGG 2 cut(s) 462, 1936
EcoT22I ATGCAT 1 cut(s) 1695
EcoT38I GRGCYC 2 cut(s) 1386, 1936
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 2 cut(s) 462, 1936
FalI AAGNNNNNCTT 2 cut(s) 1535, 1567
FaqI GGGAC 7 cut(s) 53, 181, 457, 604, 1016, 1649, 2273
FauI CCCGC 2 cut(s) 441, 445
FauNDI CATATG 2 cut(s) 943, 1780
FbaI TGATCA 2 cut(s) 2053, 2368
FblI GTMKAC 1 cut(s) 1245
Fnu4HI GCNGC 6 cut(s) 64, 92, 95, 98, 1948, 2432
FokI GGATG 7 cut(s) 734, 1124, 1654, 1733, 2023, 2306, 2353
FriOI GRGCYC 2 cut(s) 1386, 1936
Fsp4HI GCNGC 6 cut(s) 64, 92, 95, 98, 1948, 2432
FspBI CTAG 6 cut(s) 1089, 1293, 1521, 2126, 2231, 2390
GlaI GCGC 1 cut(s) 90
GluI GCNGC 6 cut(s) 64, 92, 95, 98, 1948, 2432
GsaI CCCAGC 1 cut(s) 1353
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 2 cut(s) 1348, 2130
HapII CCGG 4 cut(s) 86, 127, 719, 2291
HgaI GACGC 2 cut(s) 55, 998
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 89
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 4 cut(s) 297, 417, 1620, 2352
HinfI GANTC 4 cut(s) 529, 925, 1301, 1496
HpaII CCGG 4 cut(s) 86, 127, 719, 2291
HphI GGTGA 3 cut(s) 1798, 1841, 1864
Hpy166II GTNNAC 7 cut(s) 256, 347, 727, 735, 1246, 1615, 2228
Hpy188I TCNGA 4 cut(s) 493, 936, 1408, 2033
Hpy188III TCNNGA 6 cut(s) 25, 203, 625, 719, 2051, 2209
Hpy8I GTNNAC 7 cut(s) 256, 347, 727, 735, 1246, 1615, 2228
Hpy99I CGWCG 2 cut(s) 233, 992
HpyAV CCTTC 6 cut(s) 6, 763, 1000, 1957, 2057, 2059
HpyCH4III ACNGT 7 cut(s) 542, 686, 1462, 1805, 1829, 1910, 2206
HpyCH4IV ACGT 2 cut(s) 73, 2178
HpyF10VI GCNNNNNNNGC 8 cut(s) 60, 88, 97, 296, 425, 749, 1204, 2450
HpyF3I CTNAG 9 cut(s) 8, 78, 546, 1203, 1213, 1264, 1407, 1416, 1857
HpySE526I ACGT 2 cut(s) 73, 2178
Hsp92I GRCGYC 1 cut(s) 89
HspAI GCGC 1 cut(s) 89
KasI GGCGCC 1 cut(s) 88
Kpn2I TCCGGA 1 cut(s) 718
Ksp22I TGATCA 2 cut(s) 2053, 2368
Kzo9I GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
LmnI GCTCC 4 cut(s) 4, 826, 957, 1931
Lsp1109I GCAGC 3 cut(s) 50, 1934, 2418
LweI GCATC 6 cut(s) 202, 1421, 1714, 2026, 2284, 2443
MaeI CTAG 6 cut(s) 1089, 1293, 1521, 2126, 2231, 2390
MaeII ACGT 2 cut(s) 73, 2178
MaeIII GTNAC 6 cut(s) 832, 1132, 1147, 1462, 1852, 2299
MalI GATC 6 cut(s) 178, 392, 499, 1568, 2055, 2370
MboI GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
MboII GAAGA 7 cut(s) 211, 885, 1844, 1986, 2160, 2255, 2360
MfeI CAATTG 1 cut(s) 1320
MflI RGATCY 3 cut(s) 176, 497, 1566
MhlI GDGCHC 3 cut(s) 954, 1386, 1936
Mly113I GGCGCC 1 cut(s) 89
MmeI TCCRAC 3 cut(s) 498, 954, 2011
Mph1103I ATGCAT 1 cut(s) 1695
MroI TCCGGA 1 cut(s) 718
MroXI GAANNNNTTC 3 cut(s) 16, 1402, 2046
MslI CAYNNNNRTG 2 cut(s) 467, 2207
MspA1I CMGCKG 2 cut(s) 440, 1870
MspI CCGG 4 cut(s) 86, 127, 719, 2291
MspR9I CCNGG 2 cut(s) 128, 2291
MunI CAATTG 1 cut(s) 1320
Mva1269I GAATGC 1 cut(s) 1402
MwoI GCNNNNNNNGC 8 cut(s) 60, 88, 97, 296, 425, 749, 1204, 2450
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 2 cut(s) 128, 2291
NcoI CCATGG 1 cut(s) 462
NdeI CATATG 2 cut(s) 943, 1780
NdeII GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
NlaIV GGNNCC 2 cut(s) 90, 1933
NmuCI GTSAC 3 cut(s) 1462, 1852, 2299
NsiI ATGCAT 1 cut(s) 1695
NspI RCATGY 3 cut(s) 1202, 1510, 2451
PaeI GCATGC 2 cut(s) 1202, 1510
PagI TCATGA 2 cut(s) 202, 2208
PciI ACATGT 1 cut(s) 2447
PctI GAATGC 1 cut(s) 1402
PdmI GAANNNNTTC 3 cut(s) 16, 1402, 2046
PfeI GAWTC 4 cut(s) 529, 925, 1301, 1496
PflFI GACNNNGTC 1 cut(s) 1250
PfoI TCCNGGA 1 cut(s) 2289
PkrI GCNGC 6 cut(s) 65, 93, 96, 99, 1949, 2433
PluTI GGCGCC 1 cut(s) 92
PscI ACATGT 1 cut(s) 2447
PsiI TTATAA 2 cut(s) 1887, 1898
PspFI CCCAGC 1 cut(s) 1349
PspN4I GGNNCC 2 cut(s) 90, 1933
PspPI GGNCC 2 cut(s) 664, 1347
PsrI GAACNNNNNNTAC 4 cut(s) 123, 155, 2136, 2168
PstI CTGCAG 1 cut(s) 1170
PsuI RGATCY 3 cut(s) 176, 497, 1566
PsyI GACNNNGTC 1 cut(s) 1250
PvuII CAGCTG 1 cut(s) 1870
RsaI GTAC 9 cut(s) 164, 348, 544, 912, 1087, 1802, 1912, 1984, 2181
RsaNI GTAC 9 cut(s) 163, 347, 543, 911, 1086, 1801, 1911, 1983, 2180
RseI CAYNNNNRTG 2 cut(s) 467, 2207
SatI GCNGC 6 cut(s) 64, 92, 95, 98, 1948, 2432
Sau3AI GATC 6 cut(s) 176, 390, 497, 1566, 2053, 2368
Sau96I GGNCC 2 cut(s) 664, 1347
ScaI AGTACT 1 cut(s) 1087
ScrFI CCNGG 2 cut(s) 128, 2291
SduI GDGCHC 3 cut(s) 954, 1386, 1936
SfaNI GCATC 6 cut(s) 202, 1421, 1714, 2026, 2284, 2443
SfcI CTRYAG 3 cut(s) 1166, 1242, 1804
SfoI GGCGCC 1 cut(s) 90
SinI GGWCC 1 cut(s) 664
SmiMI CAYNNNNRTG 2 cut(s) 467, 2207
SmlI CTYRAG 2 cut(s) 623, 1529
SmoI CTYRAG 2 cut(s) 623, 1529
SpeI ACTAGT 1 cut(s) 1088
SphI GCATGC 2 cut(s) 1202, 1510
SsiI CCGC 8 cut(s) 92, 95, 98, 394, 434, 438, 486, 662
SspDI GGCGCC 1 cut(s) 88
SspI AATATT 1 cut(s) 2020
SspMI CTAG 6 cut(s) 1089, 1293, 1521, 2126, 2231, 2390
StyD4I CCNGG 2 cut(s) 126, 2289
StyI CCWWGG 2 cut(s) 462, 1936
TaaI ACNGT 7 cut(s) 542, 686, 1462, 1805, 1829, 1910, 2206
TaiI ACGT 2 cut(s) 76, 2181
TaqI TCGA 2 cut(s) 26, 210
TatI WGTACW 4 cut(s) 346, 542, 1085, 1910
TauI GCSGC 3 cut(s) 94, 97, 100
TfiI GAWTC 4 cut(s) 529, 925, 1301, 1496
TscAI CASTG 7 cut(s) 823, 1104, 1237, 1314, 1467, 2104, 2209
TseFI GTSAC 3 cut(s) 1462, 1852, 2299
TseI GCWGC 3 cut(s) 63, 1947, 2431
Tsp45I GTSAC 3 cut(s) 1462, 1852, 2299
TspGWI ACGGA 3 cut(s) 217, 263, 606
TspRI CASTG 7 cut(s) 823, 1104, 1237, 1314, 1467, 2104, 2209
Tth111I GACNNNGTC 1 cut(s) 1250
VpaK11BI GGWCC 1 cut(s) 664
XagI CCTNNNNNAGG 1 cut(s) 1166
XapI RAATTY 6 cut(s) 206, 646, 1141, 1313, 1656, 1873
XceI RCATGY 3 cut(s) 1202, 1510, 2451
XmiI GTMKAC 1 cut(s) 1245
XmnI GAANNNNTTC 3 cut(s) 16, 1402, 2046
XspI CTAG 6 cut(s) 1089, 1293, 1521, 2126, 2231, 2390
ZrmI AGTACT 1 cut(s) 1087
Zsp2I ATGCAT 1 cut(s) 1695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.