pycom16g16280

5'-nucleotidase surE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006194
Physical Location & Seq
Reverse (-)
12216318 .. 12220408
4091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16280.7

Sequence Viewer

Length: 1080 bp
ATGAATTTTAAATTACAGTATCTTAAAACATCATTATTGAAATATTCAAGTTGGAATTTTATTTGCTTTCTTTCATTTTTAACTCTTCCTCCCTCTCACTCTGTCTTCTCCTCCAAAATTCCGAACTTTAACGAAACCAAAATACAGACAAACAAGGAAATGGAAAACACCACTGTCATCTCCGCCGACAAGCCCACCGTGATGGTCACAAACGACGACGGAATCGAAGGCCCTGGCATTCTCGCTCTCGTCCAAGTCCTCGTATCCACCCATCGCTACAATGTCCTCGTCTGTGCCCCCGACTCGGAGAAGTCGGCAGTGAGTCACTGCATCACATGGCTCCATCCGATTGCTGCCAACAAAGTGCATATCAATGGAGCAACAGTGTATGCTGTTTCTGGATCTCCAGCTGATTGTGCTTCTTTGGGTGTTTCCAAAACGCTTTTTCCTTCAATTCCTGACCTGGTAATTAGTGGCATAAACCAGGGCAGCAACTGCGGTTATCACATCGTTTACTCTGGAACGGTGGCTGGTGCCCGTGAGGCCTTTCTTAACGGTGTACCTGCTATCTCTATATCATATGATTGGGTTAGAGGTAAGAGCAACATTAATGACTTCACACTTGCTGCCGAGGCTTGCTTACCGATCATGAATGGAGTACTGGTGGAATTAAGAAACAAAACCTATCCTCAAACATGTTTCTTTAGGAATGTCGATCTTCCATGTAATGTTGCTAATCATAAGGGGTATAAGCTGACTAAGCAAGGCAAAAGTATAATAAAAATGGGGTGGAGGCAAGTTACTTCTGACACGGAAGGTGGAAAAATGTTGTCAACAATGGTAAATTCAACTGCATCAGCTGAAAGTGATACATCAACCACGCCAGCTGAAAATCTTTACTTCGCCAGAGAAGTAAGGGGTGCCCAAGTTGATGAGGATGATGACCACTGCTTTCTTCAAGAAGGATATATTACTGTCACTCCTCTTGGTGCCATAACAAATGCTGAGATAGACTGCCACACCTACTTAAAAGAGTGGCTTCCAAAAGTGGTTGAGCGTTCCTCTCCATCAGCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
SurE

Protein Analysis

360

Amino Acids

39.18

Weight (kDa)

6.14

Isoelectric Point (pI)

29.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1078
Acc36I ACCTGC 1 cut(s) 571
AccB1I GGYRCC 3 cut(s) 533, 920, 989
AciI CCGC 2 cut(s) 183, 498
AclWI GGATC 1 cut(s) 409
AcsI RAATTY 4 cut(s) 4, 55, 117, 844
AfaI GTAC 2 cut(s) 561, 660
AfiI CCNNNNNNNGG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 695
AgsI TTSAA 5 cut(s) 40, 48, 453, 849, 959
AjnI CCWGG 3 cut(s) 232, 462, 483
AluBI AGCT 5 cut(s) 410, 754, 860, 887, 1073
AluI AGCT 5 cut(s) 410, 754, 860, 887, 1073
AlwI GGATC 1 cut(s) 409
AlwNI CAGNNNCTG 1 cut(s) 495
AoxI GGCC 2 cut(s) 229, 543
ApeKI GCWGC 3 cut(s) 353, 489, 626
ApoI RAATTY 4 cut(s) 4, 55, 117, 844
AseI ATTAAT 1 cut(s) 609
AspS9I GGNCC 1 cut(s) 230
BaeGI GKGCMC 3 cut(s) 298, 538, 925
BanI GGYRCC 3 cut(s) 533, 920, 989
BbsI GAAGAC 1 cut(s) 97
BbvI GCAGC 3 cut(s) 340, 501, 613
BccI CCATC 4 cut(s) 196, 279, 351, 1075
BciT130I CCWGG 3 cut(s) 234, 464, 485
BciVI GTATCC 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 571
BfuI GTATCC 1 cut(s) 274
BglI GCCNNNNNGGC 1 cut(s) 542
BisI GCNGC 3 cut(s) 354, 490, 627
BlsI GCNGC 3 cut(s) 355, 491, 628
BmcAI AGTACT 1 cut(s) 660
Bme1390I CCNGG 3 cut(s) 234, 464, 485
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 4 cut(s) 341, 535, 922, 991
BmrFI CCNGG 3 cut(s) 234, 464, 485
BmsI GCATC 2 cut(s) 339, 863
BpiI GAAGAC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 1046, 1078
BpmI CTGGAG 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 232, 484, 630
BsaXI ACNNNNNCTCC 8 cut(s) 73, 103, 369, 399, 648, 678, 964, 994
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 666
BseBI CCWGG 3 cut(s) 234, 464, 485
BseDI CCNNGG 3 cut(s) 232, 484, 630
BseGI GGATG 2 cut(s) 343, 943
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 996
BseNI ACTGG 1 cut(s) 666
BseRI GAGGAG 2 cut(s) 100, 972
BseSI GKGCMC 3 cut(s) 298, 538, 925
BseXI GCAGC 3 cut(s) 340, 501, 613
BshFI GGCC 2 cut(s) 231, 545
BshNI GGYRCC 3 cut(s) 533, 920, 989
BslI CCNNNNNNNGG 1 cut(s) 304
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 2 cut(s) 231, 545
Bsp1286I GDGCHC 3 cut(s) 298, 538, 925
Bsp143I GATC 3 cut(s) 401, 645, 715
BspACI CCGC 2 cut(s) 183, 498
BspANI GGCC 2 cut(s) 231, 545
BspCNI CTCAG 1 cut(s) 997
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 4 cut(s) 341, 535, 922, 991
BspMI ACCTGC 1 cut(s) 571
BspPI GGATC 1 cut(s) 409
BspT107I GGYRCC 3 cut(s) 533, 920, 989
BsrI ACTGG 1 cut(s) 666
BssECI CCNNGG 3 cut(s) 232, 484, 630
BssMI GATC 3 cut(s) 401, 645, 715
Bst2UI CCWGG 3 cut(s) 234, 464, 485
Bst4CI ACNGT 7 cut(s) 18, 175, 199, 385, 526, 557, 976
Bst6I CTCTTC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 495
BstC8I GCNNGC 2 cut(s) 637, 885
BstDEI CTNAG 2 cut(s) 759, 1005
BstF5I GGATG 2 cut(s) 343, 943
BstKTI GATC 3 cut(s) 404, 648, 718
BstMBI GATC 3 cut(s) 401, 645, 715
BstMWI GCNNNNNNNGC 5 cut(s) 416, 495, 542, 632, 760
BstNI CCWGG 3 cut(s) 234, 464, 485
BstNSI RCATGY 1 cut(s) 699
BstSCI CCNGG 3 cut(s) 232, 462, 483
BstSLI GKGCMC 3 cut(s) 298, 538, 925
BstV1I GCAGC 3 cut(s) 340, 501, 613
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 401
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 401
BsuI GTATCC 1 cut(s) 274
BsuRI GGCC 2 cut(s) 231, 545
BtgZI GCGATG 1 cut(s) 257
BtsCI GGATG 2 cut(s) 343, 943
BtsI GCAGTG 3 cut(s) 324, 325, 946
BtsIMutI CAGTG 5 cut(s) 171, 324, 325, 390, 946
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 2 cut(s) 637, 885
CaiI CAGNNNCTG 1 cut(s) 495
CciI TCATGA 1 cut(s) 648
Cfr13I GGNCC 1 cut(s) 230
CsiI ACCWGGT 1 cut(s) 462
Csp6I GTAC 2 cut(s) 560, 659
CviAII CATG 4 cut(s) 336, 649, 696, 723
CviQI GTAC 2 cut(s) 560, 659
DdeI CTNAG 2 cut(s) 759, 1005
DpnI GATC 3 cut(s) 403, 647, 717
DpnII GATC 3 cut(s) 401, 645, 715
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
EciI GGCGGA 1 cut(s) 172
Eco147I AGGCCT 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 3 cut(s) 232, 462, 483
FaeI CATG 4 cut(s) 339, 652, 699, 726
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FatI CATG 4 cut(s) 335, 648, 695, 722
FauNDI CATATG 1 cut(s) 580
Fnu4HI GCNGC 3 cut(s) 354, 490, 627
FokI GGATG 2 cut(s) 330, 950
Fsp4HI GCNGC 3 cut(s) 354, 490, 627
GluI GCNGC 3 cut(s) 354, 490, 627
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 2 cut(s) 231, 545
Hin1II CATG 4 cut(s) 339, 652, 699, 726
HincII GTYRAC 1 cut(s) 834
HindII GTYRAC 1 cut(s) 834
HinfI GANTC 3 cut(s) 222, 302, 322
Hpy166II GTNNAC 3 cut(s) 514, 560, 834
Hpy188I TCNGA 4 cut(s) 123, 307, 348, 808
Hpy188III TCNNGA 5 cut(s) 399, 458, 519, 649, 959
Hpy8I GTNNAC 3 cut(s) 514, 560, 834
Hpy99I CGWCG 2 cut(s) 218, 221
HpyAV CCTTC 4 cut(s) 221, 459, 809, 956
HpyCH4III ACNGT 7 cut(s) 18, 175, 199, 385, 526, 557, 976
HpyCH4V TGCA 3 cut(s) 330, 367, 854
HpyF10VI GCNNNNNNNGC 5 cut(s) 416, 495, 542, 632, 760
HpyF3I CTNAG 2 cut(s) 759, 1005
Hsp92II CATG 4 cut(s) 339, 652, 699, 726
Kzo9I GATC 3 cut(s) 401, 645, 715
LmnI GCTCC 2 cut(s) 345, 377
Lsp1109I GCAGC 3 cut(s) 340, 501, 613
LweI GCATC 2 cut(s) 339, 863
MabI ACCWGGT 1 cut(s) 462
MaeIII GTNAC 4 cut(s) 205, 323, 799, 976
MalI GATC 3 cut(s) 403, 647, 717
MboI GATC 3 cut(s) 401, 645, 715
MboII GAAGA 4 cut(s) 77, 97, 710, 947
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 3 cut(s) 298, 538, 925
MluCI AATT 8 cut(s) 4, 11, 55, 117, 453, 468, 668, 844
MlyI GAGTC 2 cut(s) 296, 331
MmeI TCCRAC 1 cut(s) 32
MseI TTAA 8 cut(s) 9, 24, 80, 129, 552, 609, 671, 1028
MslI CAYNNNNRTG 2 cut(s) 200, 372
MspA1I CMGCKG 3 cut(s) 410, 860, 887
MspR9I CCNGG 3 cut(s) 234, 464, 485
Mva1269I GAATGC 1 cut(s) 237
MvaI CCWGG 3 cut(s) 234, 464, 485
MwoI GCNNNNNNNGC 5 cut(s) 416, 495, 542, 632, 760
NdeI CATATG 1 cut(s) 580
NdeII GATC 3 cut(s) 401, 645, 715
NlaIII CATG 4 cut(s) 339, 652, 699, 726
NlaIV GGNNCC 4 cut(s) 341, 535, 922, 991
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 3 cut(s) 205, 323, 976
NspI RCATGY 1 cut(s) 699
PagI TCATGA 1 cut(s) 648
PceI AGGCCT 1 cut(s) 545
PciI ACATGT 1 cut(s) 695
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 222
PkrI GCNGC 3 cut(s) 355, 491, 628
PleI GAGTC 2 cut(s) 296, 330
PpsI GAGTC 2 cut(s) 296, 330
PscI ACATGT 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 609
PsiI TTATAA 1 cut(s) 1078
Psp6I CCWGG 3 cut(s) 232, 462, 483
PspGI CCWGG 3 cut(s) 232, 462, 483
PspN4I GGNNCC 4 cut(s) 341, 535, 922, 991
PspPI GGNCC 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 495
PsuI RGATCY 1 cut(s) 401
PvuII CAGCTG 3 cut(s) 410, 860, 887
RsaI GTAC 2 cut(s) 561, 660
RsaNI GTAC 2 cut(s) 560, 659
RseI CAYNNNNRTG 2 cut(s) 200, 372
SaqAI TTAA 8 cut(s) 9, 24, 80, 129, 552, 609, 671, 1028
SatI GCNGC 3 cut(s) 354, 490, 627
Sau3AI GATC 3 cut(s) 401, 645, 715
Sau96I GGNCC 1 cut(s) 230
ScaI AGTACT 1 cut(s) 660
SchI GAGTC 2 cut(s) 296, 331
ScrFI CCNGG 3 cut(s) 234, 464, 485
SduI GDGCHC 3 cut(s) 298, 538, 925
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 2 cut(s) 339, 863
SmiMI CAYNNNNRTG 2 cut(s) 200, 372
Sse9I AATT 8 cut(s) 4, 11, 55, 117, 453, 468, 668, 844
SseBI AGGCCT 1 cut(s) 545
SsiI CCGC 2 cut(s) 183, 498
SspI AATATT 1 cut(s) 44
StuI AGGCCT 1 cut(s) 545
StyD4I CCNGG 3 cut(s) 232, 462, 483
TaaI ACNGT 7 cut(s) 18, 175, 199, 385, 526, 557, 976
TaqI TCGA 2 cut(s) 225, 714
TasI AATT 8 cut(s) 4, 11, 55, 117, 453, 468, 668, 844
TatI WGTACW 1 cut(s) 658
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 8 cut(s) 9, 24, 80, 129, 552, 609, 671, 1028
Tru9I TTAA 8 cut(s) 9, 24, 80, 129, 552, 609, 671, 1028
TscAI CASTG 5 cut(s) 178, 324, 332, 390, 953
TseFI GTSAC 3 cut(s) 205, 323, 976
TseI GCWGC 3 cut(s) 353, 489, 626
Tsp45I GTSAC 3 cut(s) 205, 323, 976
TspDTI ATGAA 3 cut(s) 17, 63, 665
TspGWI ACGGA 2 cut(s) 234, 827
TspRI CASTG 5 cut(s) 178, 324, 332, 390, 953
VspI ATTAAT 1 cut(s) 609
XapI RAATTY 4 cut(s) 4, 55, 117, 844
XceI RCATGY 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.