FvH4_4g16175

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014716
Physical Location & Seq
Reverse (-)
20131416 .. 20133120
1705 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16175.t1

Sequence Viewer

Length: 990 bp
ATGTATGAAGATATTTGTTTTCAGTTACCAGTTGAAGGTGAATCCCCTAGTTTAATGAATGTAGATGATAGGTATCAGAAAAAGATTTCAAACGCTGAAGTTCCTCTATACCCTGGTTGTACAAAGTACTCAAAACTTTCAACCACCTTAATTATGTATACGTTGAAAGCTGAATCTGGTCTATCTAATTCTGGCTTTGATAAGTTCATGAGTGTGTTTGAAGATATGCTACCAGAGGCAAATACTTTCCCCGAGTCACTGTCCACAGTCAAGAAAACACTTAAAGATTTTGATTTAGGGTATGAGAAGATAGATGCATGTGTCAATGATTGTATCTTGTTTAGGGGAAAAGACTATGAGAACTTACAAAAATGTCCTGAATGTGATGCTCCGAGGTATAAAGTAAACTCACGTACTGGCAAGATAAAAGGAGTTGGTGCAAAAGTATTACGCTACTTTCCTATAATTCCAAGACTTCAACGGATGTTTCTTTCAGCATCTGTATGCCCTAAACAACCTGGAAATGACATTGATGTATACTTGCAGCCCCTTATAGATGACTTAAAACTGCTGTGGGATGGTGTTGACATGTATGATGCCTATTCTAAAGCCATGTTTAACCTCAGAGGTATCCTAATGTGGACTATTAATGATTTCCCGGCTTATAGGAATTTATCTGGGTTAACCAATAAAGGAGAATTTGCTTGTCCTGTTTGTGGTCCTGAAACTTGCTCTTACTGGCTACCACTTGCTCATGTGAAGGAAAAATCAAAAATTCGGGTAGCTTCACGTAGAGATCTAGAGTTACAAGAATTAATGGAAGATGTTCCAGAAGAAGCAAGAGAAGATAAGCTGGATTCTTCTGGACCTTACACCTTTACAAAGTCTGAGAAGGCAAAATTTCTTAGAGGCTGTGGATGCAAAGGTTTCCTGATGGATATTGCTCCAATATTGGAAACTGCATCAAGATTAAAGAGAGCAAAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

330

Amino Acids

37.38

Weight (kDa)

6.64

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_21 PF02992 169 - 273 1.6e-29 Transposase family tnp2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 158, 537
AcsI RAATTY 5 cut(s) 670, 698, 774, 899, 983
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 3 cut(s) 121, 128, 415
AfiI CCNNNNNNNGG 2 cut(s) 35, 716
AflIII ACRYGT 1 cut(s) 588
AgsI TTSAA 6 cut(s) 35, 90, 141, 166, 221, 479
AjnI CCWGG 2 cut(s) 112, 517
AluBI AGCT 3 cut(s) 170, 785, 853
AluI AGCT 3 cut(s) 170, 785, 853
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 251
ApeKI GCWGC 1 cut(s) 544
ApoI RAATTY 5 cut(s) 670, 698, 774, 899, 983
AseI ATTAAT 2 cut(s) 648, 815
Asp700I GAANNNNTTC 1 cut(s) 825
AspS9I GGNCC 2 cut(s) 719, 866
AsuC2I CCSGG 1 cut(s) 659
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 2 cut(s) 719, 866
BarI GAAGNNNNNNTAC 2 cut(s) 213, 245
BbvI GCAGC 1 cut(s) 556
BccI CCATC 2 cut(s) 572, 928
BciT130I CCWGG 2 cut(s) 114, 519
BciVI GTATCC 1 cut(s) 641
BcnI CCSGG 1 cut(s) 659
BfaI CTAG 3 cut(s) 48, 800, 988
BfuI GTATCC 1 cut(s) 641
BglII AGATCT 1 cut(s) 796
BisI GCNGC 1 cut(s) 545
BlsI GCNGC 1 cut(s) 546
BmcAI AGTACT 1 cut(s) 128
Bme1390I CCNGG 3 cut(s) 114, 519, 659
Bme18I GGWCC 2 cut(s) 719, 866
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 719, 866
BmrFI CCNGG 3 cut(s) 114, 519, 659
BmsI GCATC 6 cut(s) 304, 376, 506, 586, 908, 971
BpuMI CCSGG 1 cut(s) 659
BsaAI YACGTR 2 cut(s) 413, 791
BsaBI GATNNNNATC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 112, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 716
Bse1I ACTGG 3 cut(s) 29, 421, 743
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 2 cut(s) 114, 519
BseDI CCNNGG 2 cut(s) 112, 392
BseGI GGATG 3 cut(s) 489, 583, 923
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 35, 716
BseMII CTCAG 2 cut(s) 637, 879
BseNI ACTGG 3 cut(s) 29, 421, 743
BseXI GCAGC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 659
BslI CCNNNNNNNGG 2 cut(s) 35, 716
BsoBI CYCGRG 1 cut(s) 251
Bsp1407I TGTACA 1 cut(s) 119
Bsp143I GATC 1 cut(s) 796
BspCNI CTCAG 2 cut(s) 636, 880
BspHI TCATGA 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 119
BsrI ACTGG 3 cut(s) 29, 421, 743
BssECI CCNNGG 2 cut(s) 112, 392
BssMI GATC 1 cut(s) 796
BssNAI GTATAC 2 cut(s) 159, 538
Bst1107I GTATAC 2 cut(s) 159, 538
Bst2UI CCWGG 2 cut(s) 114, 519
Bst4CI ACNGT 2 cut(s) 261, 268
BstAUI TGTACA 1 cut(s) 119
BstBAI YACGTR 2 cut(s) 413, 791
BstDEI CTNAG 3 cut(s) 623, 888, 905
BstF5I GGATG 3 cut(s) 489, 583, 923
BstKTI GATC 1 cut(s) 799
BstMBI GATC 1 cut(s) 796
BstMWI GCNNNNNNNGC 1 cut(s) 918
BstNI CCWGG 2 cut(s) 114, 519
BstNSI RCATGY 2 cut(s) 321, 592
BstSCI CCNGG 3 cut(s) 112, 517, 657
BstV1I GCAGC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
BstZ17I GTATAC 2 cut(s) 159, 538
BsuI GTATCC 1 cut(s) 641
BtsCI GGATG 3 cut(s) 489, 583, 923
BtsIMutI CAGTG 1 cut(s) 257
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 719, 866
Csp6I GTAC 3 cut(s) 120, 127, 414
CviAII CATG 5 cut(s) 208, 318, 589, 613, 755
CviJI RGCY 9 cut(s) 170, 195, 547, 611, 662, 742, 785, 853, 912
CviKI_1 RGCY 9 cut(s) 170, 195, 547, 611, 662, 742, 785, 853, 912
CviQI GTAC 3 cut(s) 120, 127, 414
DdeI CTNAG 3 cut(s) 623, 888, 905
DpnI GATC 1 cut(s) 798
DpnII GATC 1 cut(s) 796
Eco47I GGWCC 2 cut(s) 719, 866
Eco57I CTGAAG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 112, 517
EcoT22I ATGCAT 1 cut(s) 319
FaeI CATG 5 cut(s) 211, 321, 592, 616, 758
FatI CATG 5 cut(s) 207, 317, 588, 612, 754
FblI GTMKAC 2 cut(s) 158, 537
Fnu4HI GCNGC 1 cut(s) 545
FokI GGATG 3 cut(s) 496, 590, 930
Fsp4HI GCNGC 1 cut(s) 545
FspBI CTAG 3 cut(s) 48, 800, 988
GluI GCNGC 1 cut(s) 545
HapII CCGG 1 cut(s) 659
Hin1II CATG 5 cut(s) 211, 321, 592, 616, 758
HincII GTYRAC 2 cut(s) 586, 684
HindII GTYRAC 2 cut(s) 586, 684
HinfI GANTC 4 cut(s) 41, 173, 254, 857
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 1 cut(s) 659
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 7 cut(s) 159, 264, 406, 538, 586, 642, 684
Hpy188I TCNGA 4 cut(s) 78, 393, 626, 889
Hpy188III TCNNGA 9 cut(s) 208, 271, 377, 722, 800, 830, 864, 931, 966
Hpy8I GTNNAC 7 cut(s) 159, 264, 406, 538, 586, 642, 684
HpyAV CCTTC 3 cut(s) 29, 754, 886
HpyCH4III ACNGT 2 cut(s) 261, 268
HpyCH4IV ACGT 3 cut(s) 161, 412, 790
HpyCH4V TGCA 5 cut(s) 317, 440, 544, 921, 962
HpyF10VI GCNNNNNNNGC 1 cut(s) 918
HpyF3I CTNAG 3 cut(s) 623, 888, 905
HpySE526I ACGT 3 cut(s) 161, 412, 790
Hsp92II CATG 5 cut(s) 211, 321, 592, 616, 758
KspAI GTTAAC 1 cut(s) 684
Kzo9I GATC 1 cut(s) 796
LmnI GCTCC 2 cut(s) 394, 949
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 6 cut(s) 304, 376, 506, 586, 908, 971
MaeI CTAG 3 cut(s) 48, 800, 988
MaeII ACGT 3 cut(s) 161, 412, 790
MaeIII GTNAC 3 cut(s) 24, 255, 804
MalI GATC 1 cut(s) 798
MboI GATC 1 cut(s) 796
MboII GAAGA 7 cut(s) 20, 233, 319, 833, 845, 852, 857
MflI RGATCY 1 cut(s) 796
MluCI AATT 9 cut(s) 150, 187, 465, 670, 698, 774, 812, 899, 983
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 6 cut(s) 114, 229, 387, 620, 632, 902
Mph1103I ATGCAT 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 825
MseI TTAA 9 cut(s) 53, 149, 282, 563, 618, 648, 683, 815, 971
MslI CAYNNNNRTG 2 cut(s) 212, 502
MspI CCGG 1 cut(s) 659
MspR9I CCNGG 3 cut(s) 114, 519, 659
MvaI CCWGG 2 cut(s) 114, 519
MwoI GCNNNNNNNGC 1 cut(s) 918
NciI CCSGG 1 cut(s) 659
NdeII GATC 1 cut(s) 796
NlaIII CATG 5 cut(s) 211, 321, 592, 616, 758
NmuCI GTSAC 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 319
NspI RCATGY 2 cut(s) 321, 592
PagI TCATGA 1 cut(s) 207
PciI ACATGT 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 825
PfeI GAWTC 3 cut(s) 41, 173, 857
PkrI GCNGC 1 cut(s) 546
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Ppu21I YACGTR 2 cut(s) 413, 791
PscI ACATGT 1 cut(s) 588
PshBI ATTAAT 2 cut(s) 648, 815
Psp6I CCWGG 2 cut(s) 112, 517
PspGI CCWGG 2 cut(s) 112, 517
PspPI GGNCC 2 cut(s) 719, 866
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 796
RsaI GTAC 3 cut(s) 121, 128, 415
RsaNI GTAC 3 cut(s) 120, 127, 414
RseI CAYNNNNRTG 2 cut(s) 212, 502
SaqAI TTAA 9 cut(s) 53, 149, 282, 563, 618, 648, 683, 815, 971
SatI GCNGC 1 cut(s) 545
Sau3AI GATC 1 cut(s) 796
Sau96I GGNCC 2 cut(s) 719, 866
ScaI AGTACT 1 cut(s) 128
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 3 cut(s) 114, 519, 659
SfaNI GCATC 6 cut(s) 304, 376, 506, 586, 908, 971
SinI GGWCC 2 cut(s) 719, 866
SmiMI CAYNNNNRTG 2 cut(s) 212, 502
Sse9I AATT 9 cut(s) 150, 187, 465, 670, 698, 774, 812, 899, 983
SspI AATATT 1 cut(s) 951
SspMI CTAG 3 cut(s) 48, 800, 988
StyD4I CCNGG 3 cut(s) 112, 517, 657
TaaI ACNGT 2 cut(s) 261, 268
TaiI ACGT 3 cut(s) 164, 415, 793
TasI AATT 9 cut(s) 150, 187, 465, 670, 698, 774, 812, 899, 983
TatI WGTACW 2 cut(s) 119, 126
TfiI GAWTC 3 cut(s) 41, 173, 857
Tru1I TTAA 9 cut(s) 53, 149, 282, 563, 618, 648, 683, 815, 971
Tru9I TTAA 9 cut(s) 53, 149, 282, 563, 618, 648, 683, 815, 971
TscAI CASTG 1 cut(s) 264
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 3 cut(s) 21, 71, 196
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 1 cut(s) 264
VpaK11BI GGWCC 2 cut(s) 719, 866
VspI ATTAAT 2 cut(s) 648, 815
XapI RAATTY 5 cut(s) 670, 698, 774, 899, 983
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 2 cut(s) 321, 592
XmiI GTMKAC 2 cut(s) 158, 537
XmnI GAANNNNTTC 1 cut(s) 825
XspI CTAG 3 cut(s) 48, 800, 988
ZrmI AGTACT 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.