Rh5DG558700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014716
Physical Location & Seq
Reverse (-)
86187844 .. 86188218
375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG558700.1

Sequence Viewer

Length: 375 bp
ATGGGGAATCCAGAAAGGATTCGTTGTCCTTGTAACAATTGTATAAGTTTCAAACGCCACAACTTTAATGTAGTATATGACCACCTGATTTGCTATGGGATAGATCCTCTTTATAGTAGGTGGACATGGCATGGGGATGAGCCAACATTACCGGATGAAAATGTTGAAATGCCTGAAACATATAGAATGTTTCAGGATGCACTTTTTGAAGATAATGATGCATTTTCTGAAGAAAATGATGTTAGGGAACCTGGAGATGAAAGAGAAGAGCTTGATTTTGGACATTTATTAGAGGAGGCAAAACGTCCTATCTATAAAGGCTCTACTTTGTCAAAAATGTCGGCAACAGTAGCTATGATGTTGATACTTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 Transpos_assoc

Protein Analysis

124

Amino Acids

14.52

Weight (kDa)

4.59

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 5 - 45 5.2e-11 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 98
AcuI CTGAAG 1 cut(s) 249
AgsI TTSAA 3 cut(s) 52, 167, 209
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 2 cut(s) 271, 353
AluI AGCT 2 cut(s) 271, 353
AlwI GGATC 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 18
BciT130I CCWGG 1 cut(s) 252
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 187, 208
BpmI CTGGAG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 151
BseBI CCWGG 1 cut(s) 252
BseGI GGATG 3 cut(s) 142, 160, 202
BseRI GAGGAG 1 cut(s) 308
BsiSI CCGG 1 cut(s) 152
Bsp143I GATC 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 1 cut(s) 98
BspQI GCTCTTC 1 cut(s) 261
BssMI GATC 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 261
BstF5I GGATG 3 cut(s) 142, 160, 202
BstKTI GATC 1 cut(s) 106
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 1 cut(s) 350
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BtsCI GGATG 3 cut(s) 142, 160, 202
CspCI CAANNNNNGTGG 2 cut(s) 71, 106
CviAII CATG 2 cut(s) 126, 131
CviJI RGCY 4 cut(s) 142, 271, 321, 353
CviKI_1 RGCY 4 cut(s) 142, 271, 321, 353
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco57I CTGAAG 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 250
EcoT22I ATGCAT 1 cut(s) 223
FaeI CATG 2 cut(s) 129, 134
FatI CATG 2 cut(s) 125, 130
FokI GGATG 3 cut(s) 149, 167, 209
GsuI CTGGAG 1 cut(s) 273
HapII CCGG 1 cut(s) 152
Hin1II CATG 2 cut(s) 129, 134
HinfI GANTC 2 cut(s) 7, 19
HpaII CCGG 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 2 cut(s) 11, 194
Hpy8I GTNNAC 1 cut(s) 123
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 2 cut(s) 200, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 350
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 2 cut(s) 129, 134
Kzo9I GATC 1 cut(s) 103
LguI GCTCTTC 1 cut(s) 261
LpnPI CCDG 7 cut(s) 24, 98, 165, 179, 186, 237, 264
LweI GCATC 2 cut(s) 187, 208
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 32
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 3 cut(s) 221, 242, 278
MfeI CAATTG 1 cut(s) 37
MflI RGATCY 1 cut(s) 103
MluCI AATT 1 cut(s) 37
MnlI CCTC 3 cut(s) 117, 286, 289
Mph1103I ATGCAT 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 252
MunI CAATTG 1 cut(s) 37
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 1 cut(s) 350
NdeII GATC 1 cut(s) 103
NlaIII CATG 2 cut(s) 129, 134
NlaIV GGNNCC 1 cut(s) 249
NsiI ATGCAT 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 7, 19
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 249
PsuI RGATCY 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 261
SaqAI TTAA 1 cut(s) 66
Sau3AI GATC 1 cut(s) 103
ScrFI CCNGG 1 cut(s) 252
SetI ASST 6 cut(s) 87, 122, 253, 273, 307, 355
SfaNI GCATC 2 cut(s) 187, 208
SmiMI CAYNNNNRTG 1 cut(s) 135
Sse9I AATT 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 250
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 1 cut(s) 307
TasI AATT 1 cut(s) 37
TfiI GAWTC 2 cut(s) 7, 19
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TspDTI ATGAA 2 cut(s) 171, 273
XmnI GAANNNNTTC 1 cut(s) 18
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.