Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0004455

Physical Location & Seq

Genomic Coordinates Forward (+)

22436125 .. 22441554

Gene Structure 5430 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g18570.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGGAAATCTTTATACACCAGCAAGATCAGTACAGTAACCTTGTTCCAGGGCTGTATGAATTTGATGCTGAAGTTGTTGAGACAGAGACGAATTTGTCCATTCCTGTTCCGGATTTGCACTTTGAAGAAGTGGCGGCTGGAATCCAGCTGTTTTCTTTTACCAATCTGGAACCAGGCAACTTTCTGTTGACTATATCAGATATGAAGCATGATAAAAGCATCTCTAATATGCCATATGCATATACCGTCTTTGTTGGCTATTGCAATGGGACAAACAGTGTTGTTAATGGAACCGGTCTAAATAGCTCCATCGCAGGAGAAAGATCAGAATTCTCAGTTTATTTGAATGATGCTTATCAAAATCCCTCTCCAGTTGGAATTGAGAGGCTTGAAATACAAATCTTAAGGGAAATTGACTTCTACCATGTGAAGTCTGGCATATTTCCTATCCAGATCATCAATGGGAGTGGACCTGCTCAAGGAATAAGATATGGTACAACCAACAAAATAGAATTAGCTCCTTCACCATCTGTGACCTCGATTAATTCTACTGTTGGGGGCGTCAGCACTTTGGCAACTGCTTTCAATGTGGTATATACACCTGAGAAGAGTGGGACTTACAAAATTCTTGTATTTTGTGGAAATATTGTGCTGAATGGAGGCCAACCCTTTTACAAGCAAGTTATAGCAGGGAGTGTTGATCCATTATGCTCAACAGTAGTCAAATTTTCTCCCAAGGTGGCAAGGATGATTAATAATGAAGTTGTAGTGCAACTTGGGGATTCATTTTTCAACCCTGTCTTGTCACACCAATCTGGGCTGAAACTAGAGATTGTTTCTGTAAACAGTTCTGGCTTTTCAAGTTCCTTGTTTGTGGACAATAACGATGGATCATACACTGTGCATTATATGGCTAAGGATGTTGGAACTTATGAGATCTGTGCTTCATTTGATGGCAAACGTATTTCTTCCTGCCCCTTTGGGGTCAATGTCTACAATAGCGAATATTTTCCTAAAGCCGAAAATGATACAGTTGCTGTGTGGGAGGACGAGTCAATCGCTTTTGATGTTCTGGCGAATGATTACTTTGCTGGAAATAATGCTAGTGTTATTGAATTCTCCAAGCCAGGTCATGGTTCCCTTATCGAGTATGGGAAGCTCCTCCGGTATACACCTTATAAAGACTATTATGGCAATGATTCCTTTGTGTACATTATGTCTGATATAAACGGCAATCTTGCTACTGCTCCTGTAAACATATTTATTCTCACCATCCCACCACAAGTTGTTTCTTTTCCAAGTGAATTGCAAGCCACTGAAGATGAGGTTAATCCAAAATTTGGTGGCTTCTCTGGGTTTATGATAAGATATTCTGACATGATGGAGAACATCTCTGTTAATCTTAGTGCTCAATCTGGGACAGTATATCTGTCTCCCATGCTAATGCAATTCTGGGAGCCCATTTGGAATGGACTTTCTGTATACAAAGAGGATGGAGGAGTGAACGGGTTGATATTACAAGGCAGCGTGGAAGCAATCAATTTTGCCCTTCAGTCAATTCAGTACTATGGAAATGAGAACTTCTGTGGCTATGATACAATTCGGCTCACTACGAGGAATAGGAATGGAGTAAATGATTTGCATATCCCAGTTCTTGTGGAACCTATCAATGATCCTCCTTTTATTCATGCACCTGAGTTTATTATTTTGAAAAGCAATGAAGATGAGTCACTGATATATGACAGAGAAACAGACAAGTTTGAGTTCTACATTGGAGATCCAGATCTTCTTGCGTTCCCTGGTAACGAATCTCTTTTTCTGGTCATCTTCTCGATGGAAGTTAATGATGGATATTTGGTAACCAGCCTACCAGCTGAGCTCATCACTACAGTAGAAGTGAAGATTAAGAATAGCTACCAGTGGCTACCTCTTCAGACATATGTTACCATCTCAAAGCATTTCATGGTCAAAGCTAAGGGAATAAGATTACATGCAACAGTTGATGAATGCAACACTGTTATGCAACAGCTGTTTTATCATGGTGGAGGAGAACAAGATGCCATCTTGACAGTGACATTGAATGACCTGGGAAACTATGGGTGTTATTGTAACTGTTCCGAACATATATCAGTGCCCTTACTTGCCGAGGCTTCTCTAAATCTTATAAGAAGAAGGCCGATGAGTTCACTTGTGGCTCGTGGCTTAGGATCAGCTATTGTCATCGAGTCCAGTATAGTGTTTTCACTCGGAGCAGCACTAATATTTTTCACATGCAAATGTGCAACTATCCTTGTAAATGAAAGAAGAAAGAGAGCTACCAGTACCCCAGCTGGACCAGCAAGTGAACATAGTTTGGGCAAAAGAACTTTGACTAAAAGTTTATCAGAGAATGCAACTCACGTTGCCGCATCTTGTTCAGAGGTTGCAACTCACCTTACCGCATCTTGTTCAACTCCCTTACTTGGCAGCCAACGTCCCAACTTTCGACAACGGTCCCGCCGTCAATCCGGAAATGAGGAATCAAGCAAGGCTGCATATAAGGCTACTGGTGAACACGTTCACCAGAATCCTGCGCCTAGTTTTCAGCCCTTTTTTGTCGAGAAGGAACAAAGTGAGCCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009987 GO:0016020 GO:0016021 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0044425 GO:0044459 GO:0044464 GO:0044703 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Filamin

Protein Analysis

875

Amino Acids

96.22

Weight (kDa)

4.95

Isoelectric Point (pI)

44.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Filamin	PF00630	275 - 327	1.5e-06	Filamin/ABP280 repeat
Big_9	PF17963	338 - 420	7.9e-12	Bacterial Ig domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004455)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150
fragaria_vesca	FvH4_4g18570
prunus_persica	Prupe.1G132400_v2.0.a1 Prupe.1G132400_v2.0.a1 Prupe.1G132400_v2.0.a1 Prupe.1G132400_v2.0.a1
pyrus_communis	pycom16g14000 pycom16g14010
rosa_chinensis	RchiOBHm_Chr4g0423171
rosa_laevigata	RLG00000007579
rosa_multiflora	Rmu_sc0000744.1_g000001 Rmu_sc0008829.1_g000002 Rmu_sc0020601.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365330
rosa_rugosa	Rorug04G0184800 Rorug04G0184900
rosa_samantha	Rh4AG244300 Rh4AG244400 Rh4BG249500 Rh4BG249600 Rh4CG258600 Rh4CG258700 Rh4DG244300 Rh4DG244400
rosa_wichuraiana	Rw4G021050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1179, 2165
AasI	GACNNNNNNGTC	1 cut(s)	94
Acc36I	ACCTGC	1 cut(s)	481
AccB7I	CCANNNNNTGG	2 cut(s)	1133, 1340
AccI	GTMKAC	3 cut(s)	993, 1169, 1482
AccIII	TCCGGA	2 cut(s)	109, 2507
AciI	CCGC	4 cut(s)	134, 2406, 2439, 2497
AclWI	GGATC	5 cut(s)	695, 898, 1667, 1772, 2215
AcsI	RAATTY	7 cut(s)	59, 91, 329, 624, 725, 1115, 1337
AcuI	CTGAAG	4 cut(s)	90, 1338, 1535, 1916
AcyI	GRCGYC	1 cut(s)	561
AfaI	GTAC	5 cut(s)	32, 496, 1211, 1565, 2323
AfiI	CCNNNNNNNGG	5 cut(s)	479, 982, 1133, 1340, 2462
AflII	CTTAAG	1 cut(s)	403
AflIII	ACRYGT	1 cut(s)	2554
AgeI	ACCGGT	1 cut(s)	293
AjnI	CCWGG	5 cut(s)	46, 172, 1126, 1798, 2085
Alw21I	GWGCWC	2 cut(s)	1411, 1881
Alw26I	GTCTC	3 cut(s)	74, 80, 1437
AlwI	GGATC	5 cut(s)	695, 898, 1667, 1772, 2215
AlwNI	CAGNNNCTG	1 cut(s)	1037
Aor13HI	TCCGGA	2 cut(s)	109, 2507
AoxI	GGCC	2 cut(s)	661, 2174
ApeKI	GCWGC	4 cut(s)	1524, 2252, 2466, 2531
ApoI	RAATTY	7 cut(s)	59, 91, 329, 624, 725, 1115, 1337
AseI	ATTAAT	2 cut(s)	543, 753
AsiGI	ACCGGT	1 cut(s)	293
Asp700I	GAANNNNTTC	2 cut(s)	1008, 2556
AspLEI	GCGC	1 cut(s)	2575
AspS9I	GGNCC	3 cut(s)	470, 2333, 2493
AsuHPI	GGTGA	5 cut(s)	516, 1261, 2423, 2552, 2561
AvaII	GGWCC	3 cut(s)	470, 2333, 2493
BaeGI	GKGCMC	1 cut(s)	2136
BanII	GRGCYC	2 cut(s)	1461, 1881
BauI	CACGAG	1 cut(s)	2196
Bbv12I	GWGCWC	2 cut(s)	1411, 1881
BbvI	GCAGC	4 cut(s)	1536, 2264, 2478, 2518
BceAI	ACGGC	2 cut(s)	1246, 2484
BciT130I	CCWGG	5 cut(s)	48, 174, 1128, 1800, 2087
BcoDI	GTCTC	3 cut(s)	74, 80, 1437
BfaI	CTAG	3 cut(s)	827, 1104, 2577
BfmI	CTRYAG	1 cut(s)	1887
BfrI	CTTAAG	1 cut(s)	403
BfuAI	ACCTGC	1 cut(s)	481
BglII	AGATCT	2 cut(s)	936, 1783
BisI	GCNGC	6 cut(s)	135, 1525, 2253, 2406, 2467, 2532
BlpI	GCTNAGC	1 cut(s)	1875
BlsI	GCNGC	6 cut(s)	136, 1526, 2254, 2407, 2468, 2533
BmcAI	AGTACT	1 cut(s)	1565
Bme1390I	CCNGG	5 cut(s)	48, 174, 1128, 1800, 2087
Bme18I	GGWCC	3 cut(s)	470, 2333, 2493
BmgT120I	GGNCC	3 cut(s)	470, 2333, 2493
BmiI	GGNNCC	6 cut(s)	171, 292, 1138, 1458, 1662, 2495
BmrFI	CCNGG	5 cut(s)	48, 174, 1128, 1800, 2087
BmrI	ACTGGG	1 cut(s)	1643
BmsI	GCATC	6 cut(s)	55, 228, 340, 2047, 2417, 2450
BmuI	ACTGGG	1 cut(s)	1643
BoxI	GACNNNNGTC	1 cut(s)	2491
BplI	GAGNNNNNCTC	2 cut(s)	1376, 1408
BpmI	CTGGAG	1 cut(s)	354
Bpu10I	CCTNAGC	3 cut(s)	915, 1974, 2203
Bpu1102I	GCTNAGC	1 cut(s)	1875
BpuEI	CTTGAG	1 cut(s)	462
BsaBI	GATNNNNATC	2 cut(s)	354, 1782
BsaHI	GRCGYC	1 cut(s)	561
BsaJI	CCNNGG	5 cut(s)	47, 735, 1798, 2086, 2145
BsaWI	WCCGGW	4 cut(s)	109, 293, 1164, 2507
BsaXI	ACNNNNNCTCC	2 cut(s)	1037, 1067
Bsc4I	CCNNNNNNNGG	5 cut(s)	479, 982, 1133, 1340, 2462
Bse118I	RCCGGY	1 cut(s)	293
Bse1I	ACTGG	6 cut(s)	371, 1649, 1918, 2229, 2319, 2551
Bse3DI	GCAATG	3 cut(s)	271, 1201, 1723
Bse8I	GATNNNNATC	2 cut(s)	354, 1782
BseAI	TCCGGA	2 cut(s)	109, 2507
BseBI	CCWGG	5 cut(s)	48, 174, 1128, 1800, 2087
BseDI	CCNNGG	5 cut(s)	47, 735, 1798, 2086, 2145
BseGI	GGATG	4 cut(s)	753, 925, 1272, 1498
BseJI	GATNNNNATC	2 cut(s)	354, 1782
BseLI	CCNNNNNNNGG	5 cut(s)	479, 982, 1133, 1340, 2462
BseMI	GCAATG	3 cut(s)	271, 1201, 1723
BseMII	CTCAG	4 cut(s)	348, 594, 1686, 1866
BseNI	ACTGG	6 cut(s)	371, 1649, 1918, 2229, 2319, 2551
BseRI	GAGGAG	3 cut(s)	1151, 1512, 2061
BseSI	GKGCMC	1 cut(s)	2136
BseXI	GCAGC	4 cut(s)	1536, 2264, 2478, 2518
BseYI	CCCAGC	1 cut(s)	2326
BshFI	GGCC	2 cut(s)	663, 2176
BshTI	ACCGGT	1 cut(s)	293
BsiHKAI	GWGCWC	2 cut(s)	1411, 1881
BsiSI	CCGG	4 cut(s)	110, 294, 1165, 2508
BslFI	GGGAC	5 cut(s)	283, 628, 1432, 2460, 2479
BslI	CCNNNNNNNGG	5 cut(s)	479, 982, 1133, 1340, 2462
BsmAI	GTCTC	3 cut(s)	74, 80, 1437
BsmBI	CGTCTC	1 cut(s)	80
BsmFI	GGGAC	5 cut(s)	283, 628, 1432, 2460, 2479
BsmI	GAATGC	2 cut(s)	2012, 2395
BsnI	GGCC	2 cut(s)	663, 2176
Bsp1286I	GDGCHC	4 cut(s)	1411, 1461, 1881, 2136
Bsp13I	TCCGGA	2 cut(s)	109, 2507
Bsp1407I	TGTACA	1 cut(s)	1209
Bsp1720I	GCTNAGC	1 cut(s)	1875
BspACI	CCGC	4 cut(s)	134, 2406, 2439, 2497
BspANI	GGCC	2 cut(s)	663, 2176
BspCNI	CTCAG	4 cut(s)	347, 595, 1687, 1867
BspEI	TCCGGA	2 cut(s)	109, 2507
BspLI	GGNNCC	6 cut(s)	171, 292, 1138, 1458, 1662, 2495
BspMI	ACCTGC	1 cut(s)	481
BspPI	GGATC	5 cut(s)	695, 898, 1667, 1772, 2215
BspTI	CTTAAG	1 cut(s)	403
BsrDI	GCAATG	3 cut(s)	271, 1201, 1723
BsrFI	RCCGGY	1 cut(s)	293
BsrGI	TGTACA	1 cut(s)	1209
BsrI	ACTGG	6 cut(s)	371, 1649, 1918, 2229, 2319, 2551
BssAI	RCCGGY	1 cut(s)	293
BssECI	CCNNGG	5 cut(s)	47, 735, 1798, 2086, 2145
BssNAI	GTATAC	2 cut(s)	1170, 1483
BssNI	GRCGYC	1 cut(s)	561
BssSI	CACGAG	1 cut(s)	2196
BssT1I	CCWWGG	1 cut(s)	735
Bst1107I	GTATAC	2 cut(s)	1170, 1483
Bst2BI	CACGAG	1 cut(s)	2196
Bst2UI	CCWGG	5 cut(s)	48, 174, 1128, 1800, 2087
Bst6I	CTCTTC	2 cut(s)	602, 1935
BstACI	GRCGYC	1 cut(s)	561
BstAFI	CTTAAG	1 cut(s)	403
BstAUI	TGTACA	1 cut(s)	1209
BstC8I	GCNNGC	1 cut(s)	1311
BstDEI	CTNAG	8 cut(s)	334, 603, 915, 1403, 1695, 1875, 1974, 2203
BstEII	GGTNACC	1 cut(s)	1858
BstENI	CCTNNNNNAGG	1 cut(s)	477
BstF5I	GGATG	4 cut(s)	753, 925, 1272, 1498
BstHHI	GCGC	1 cut(s)	2575
BstMAI	GTCTC	3 cut(s)	74, 80, 1437
BstMWI	GCNNNNNNNGC	2 cut(s)	2336, 2540
BstNI	CCWGG	5 cut(s)	48, 174, 1128, 1800, 2087
BstNSI	RCATGY	2 cut(s)	1994, 2274
BstPAI	GACNNNNGTC	1 cut(s)	2491
BstPI	GGTNACC	1 cut(s)	1858
BstSCI	CCNGG	5 cut(s)	46, 172, 1126, 1798, 2085
BstSFI	CTRYAG	1 cut(s)	1887
BstSLI	GKGCMC	1 cut(s)	2136
BstV1I	GCAGC	4 cut(s)	1536, 2264, 2478, 2518
BstX2I	RGATCY	3 cut(s)	936, 1777, 1783
BstYI	RGATCY	3 cut(s)	936, 1777, 1783
BstZ17I	GTATAC	2 cut(s)	1170, 1483
BsuRI	GGCC	2 cut(s)	663, 2176
BtgZI	GCGATG	1 cut(s)	295
BtsCI	GGATG	4 cut(s)	753, 925, 1272, 1498
BtsIMutI	CAGTG	8 cut(s)	283, 897, 1314, 1730, 1925, 2013, 2076, 2136
BveI	ACCTGC	1 cut(s)	481
Cac8I	GCNNGC	1 cut(s)	1311
CaiI	CAGNNNCTG	1 cut(s)	1037
CfoI	GCGC	1 cut(s)	2575
Cfr10I	RCCGGY	1 cut(s)	293
Cfr13I	GGNCC	3 cut(s)	470, 2333, 2493
CseI	GACGC	1 cut(s)	550
Csp6I	GTAC	5 cut(s)	31, 495, 1210, 1564, 2322
CspAI	ACCGGT	1 cut(s)	293
CspCI	CAANNNNNGTGG	2 cut(s)	448, 483
CviQI	GTAC	5 cut(s)	31, 495, 1210, 1564, 2322
DdeI	CTNAG	8 cut(s)	334, 603, 915, 1403, 1695, 1875, 1974, 2203
DrdI	GACNNNNNNGTC	1 cut(s)	94
DseDI	GACNNNNNNGTC	1 cut(s)	94
Eam1104I	CTCTTC	2 cut(s)	602, 1935
EarI	CTCTTC	2 cut(s)	602, 1935
Ecl136II	GAGCTC	1 cut(s)	1879
Eco130I	CCWWGG	1 cut(s)	735
Eco24I	GRGCYC	2 cut(s)	1461, 1881
Eco47I	GGWCC	3 cut(s)	470, 2333, 2493
Eco53kI	GAGCTC	1 cut(s)	1879
Eco57I	CTGAAG	4 cut(s)	90, 1338, 1535, 1916
Eco91I	GGTNACC	1 cut(s)	1858
EcoICRI	GAGCTC	1 cut(s)	1879
EcoNI	CCTNNNNNAGG	1 cut(s)	477
EcoO65I	GGTNACC	1 cut(s)	1858
EcoRI	GAATTC	2 cut(s)	329, 1115
EcoRII	CCWGG	5 cut(s)	46, 172, 1126, 1798, 2085
EcoT14I	CCWWGG	1 cut(s)	735
EcoT22I	ATGCAT	1 cut(s)	241
EcoT38I	GRGCYC	2 cut(s)	1461, 1881
ErhI	CCWWGG	1 cut(s)	735
Esp3I	CGTCTC	1 cut(s)	80
FaqI	GGGAC	5 cut(s)	283, 628, 1432, 2460, 2479
FauI	CCCGC	1 cut(s)	2504
FauNDI	CATATG	2 cut(s)	235, 1939
FblI	GTMKAC	3 cut(s)	993, 1169, 1482
Fnu4HI	GCNGC	6 cut(s)	135, 1525, 2253, 2406, 2467, 2532
FokI	GGATG	4 cut(s)	760, 932, 1259, 1505
FriOI	GRGCYC	2 cut(s)	1461, 1881
Fsp4HI	GCNGC	6 cut(s)	135, 1525, 2253, 2406, 2467, 2532
FspBI	CTAG	3 cut(s)	827, 1104, 2577
GlaI	GCGC	1 cut(s)	2574
GluI	GCNGC	6 cut(s)	135, 1525, 2253, 2406, 2467, 2532
GsaI	CCCAGC	1 cut(s)	2330
GsuI	CTGGAG	1 cut(s)	354
HaeIII	GGCC	2 cut(s)	663, 2176
HapII	CCGG	4 cut(s)	110, 294, 1165, 2508
HgaI	GACGC	1 cut(s)	550
HhaI	GCGC	1 cut(s)	2575
Hin1I	GRCGYC	1 cut(s)	561
Hin6I	GCGC	1 cut(s)	2573
HinP1I	GCGC	1 cut(s)	2573
HincII	GTYRAC	1 cut(s)	189
HindII	GTYRAC	1 cut(s)	189
HinfI	GANTC	9 cut(s)	141, 782, 1052, 1199, 1727, 1808, 2225, 2519, 2566
HpaII	CCGG	4 cut(s)	110, 294, 1165, 2508
HphI	GGTGA	5 cut(s)	516, 1261, 2423, 2552, 2561
Hpy188I	TCNGA	9 cut(s)	199, 328, 1222, 1375, 1935, 2119, 2249, 2386, 2419
Hpy188III	TCNNGA	8 cut(s)	110, 167, 451, 1781, 1831, 2065, 2508, 2599
HpyAV	CCTTC	4 cut(s)	531, 1559, 2166, 2596
HpyCH4IV	ACGT	4 cut(s)	961, 2400, 2473, 2556
HpyF10VI	GCNNNNNNNGC	2 cut(s)	2336, 2540
HpyF3I	CTNAG	8 cut(s)	334, 603, 915, 1403, 1695, 1875, 1974, 2203
HpySE526I	ACGT	4 cut(s)	961, 2400, 2473, 2556
Hsp92I	GRCGYC	1 cut(s)	561
HspAI	GCGC	1 cut(s)	2573
Kpn2I	TCCGGA	2 cut(s)	109, 2507
LmnI	GCTCC	6 cut(s)	311, 523, 1164, 1252, 1456, 2249
Lsp1109I	GCAGC	4 cut(s)	1536, 2264, 2478, 2518
LweI	GCATC	6 cut(s)	55, 228, 340, 2047, 2417, 2450
MaeI	CTAG	3 cut(s)	827, 1104, 2577
MaeII	ACGT	4 cut(s)	961, 2400, 2473, 2556
MaeIII	GTNAC	9 cut(s)	35, 532, 804, 1728, 1802, 1858, 1942, 2071, 2108
MflI	RGATCY	3 cut(s)	936, 1777, 1783
MhlI	GDGCHC	4 cut(s)	1411, 1461, 1881, 2136
MlyI	GAGTC	3 cut(s)	1061, 1736, 2234
MmeI	TCCRAC	2 cut(s)	355, 904
Mph1103I	ATGCAT	1 cut(s)	241
MroI	TCCGGA	2 cut(s)	109, 2507
MroXI	GAANNNNTTC	2 cut(s)	1008, 2556
MseI	TTAA	8 cut(s)	285, 404, 543, 753, 1329, 1398, 1842, 1905
MslI	CAYNNNNRTG	3 cut(s)	1442, 2018, 2275
MspA1I	CMGCKG	4 cut(s)	148, 1874, 2029, 2330
MspCI	CTTAAG	1 cut(s)	403
MspI	CCGG	4 cut(s)	110, 294, 1165, 2508
MspR9I	CCNGG	5 cut(s)	48, 174, 1128, 1800, 2087
Mva1269I	GAATGC	2 cut(s)	2012, 2395
MvaI	CCWGG	5 cut(s)	48, 174, 1128, 1800, 2087
MwoI	GCNNNNNNNGC	2 cut(s)	2336, 2540
NdeI	CATATG	2 cut(s)	235, 1939
NlaIV	GGNNCC	6 cut(s)	171, 292, 1138, 1458, 1662, 2495
NmeAIII	GCCGAG	1 cut(s)	2170
NmuCI	GTSAC	4 cut(s)	532, 804, 1728, 2071
NsiI	ATGCAT	1 cut(s)	241
NspI	RCATGY	2 cut(s)	1994, 2274
PcsI	WCGNNNNNNNCGW	1 cut(s)	2497
PctI	GAATGC	2 cut(s)	2012, 2395
PdmI	GAANNNNTTC	2 cut(s)	1008, 2556
PfeI	GAWTC	6 cut(s)	141, 782, 1199, 1808, 2519, 2566
PflMI	CCANNNNNTGG	2 cut(s)	1133, 1340
PinAI	ACCGGT	1 cut(s)	293
PkrI	GCNGC	6 cut(s)	136, 1526, 2254, 2407, 2468, 2533
PleI	GAGTC	3 cut(s)	1060, 1735, 2233
PpsI	GAGTC	3 cut(s)	1060, 1735, 2233
PshAI	GACNNNNGTC	1 cut(s)	2491
PshBI	ATTAAT	2 cut(s)	543, 753
PsiI	TTATAA	2 cut(s)	1179, 2165
Psp124BI	GAGCTC	1 cut(s)	1881
Psp6I	CCWGG	5 cut(s)	46, 172, 1126, 1798, 2085
PspEI	GGTNACC	1 cut(s)	1858
PspFI	CCCAGC	1 cut(s)	2326
PspGI	CCWGG	5 cut(s)	46, 172, 1126, 1798, 2085
PspN4I	GGNNCC	6 cut(s)	171, 292, 1138, 1458, 1662, 2495
PspPI	GGNCC	3 cut(s)	470, 2333, 2493
PstNI	CAGNNNCTG	1 cut(s)	1037
PsuI	RGATCY	3 cut(s)	936, 1777, 1783
PvuII	CAGCTG	4 cut(s)	148, 1874, 2029, 2330
RsaI	GTAC	5 cut(s)	32, 496, 1211, 1565, 2323
RsaNI	GTAC	5 cut(s)	31, 495, 1210, 1564, 2322
RseI	CAYNNNNRTG	3 cut(s)	1442, 2018, 2275
SacI	GAGCTC	1 cut(s)	1881
SaqAI	TTAA	8 cut(s)	285, 404, 543, 753, 1329, 1398, 1842, 1905
SatI	GCNGC	6 cut(s)	135, 1525, 2253, 2406, 2467, 2532
Sau96I	GGNCC	3 cut(s)	470, 2333, 2493
ScaI	AGTACT	1 cut(s)	1565
SchI	GAGTC	3 cut(s)	1061, 1736, 2234
ScrFI	CCNGG	5 cut(s)	48, 174, 1128, 1800, 2087
SduI	GDGCHC	4 cut(s)	1411, 1461, 1881, 2136
SfaNI	GCATC	6 cut(s)	55, 228, 340, 2047, 2417, 2450
SfcI	CTRYAG	1 cut(s)	1887
SinI	GGWCC	3 cut(s)	470, 2333, 2493
SmiMI	CAYNNNNRTG	3 cut(s)	1442, 2018, 2275
SmlI	CTYRAG	2 cut(s)	403, 477
SmoI	CTYRAG	2 cut(s)	403, 477
SsiI	CCGC	4 cut(s)	134, 2406, 2439, 2497
SspI	AATATT	3 cut(s)	646, 1007, 2262
SspMI	CTAG	3 cut(s)	827, 1104, 2577
SstI	GAGCTC	1 cut(s)	1881
StyD4I	CCNGG	5 cut(s)	46, 172, 1126, 1798, 2085
StyI	CCWWGG	1 cut(s)	735
TaiI	ACGT	4 cut(s)	964, 2403, 2476, 2559
TaqI	TCGA	6 cut(s)	539, 1146, 1832, 2223, 2485, 2598
TatI	WGTACW	3 cut(s)	30, 1209, 1563
TauI	GCSGC	2 cut(s)	137, 2408
TfiI	GAWTC	6 cut(s)	141, 782, 1199, 1808, 2519, 2566
Tru1I	TTAA	8 cut(s)	285, 404, 543, 753, 1329, 1398, 1842, 1905
Tru9I	TTAA	8 cut(s)	285, 404, 543, 753, 1329, 1398, 1842, 1905
TscAI	CASTG	8 cut(s)	283, 904, 1321, 1737, 1925, 2020, 2076, 2136
TseFI	GTSAC	4 cut(s)	532, 804, 1728, 2071
TseI	GCWGC	4 cut(s)	1524, 2252, 2466, 2531
Tsp45I	GTSAC	4 cut(s)	532, 804, 1728, 2071
TspRI	CASTG	8 cut(s)	283, 904, 1321, 1737, 1925, 2020, 2076, 2136
Van91I	CCANNNNNTGG	2 cut(s)	1133, 1340
Vha464I	CTTAAG	1 cut(s)	403
VpaK11BI	GGWCC	3 cut(s)	470, 2333, 2493
VspI	ATTAAT	2 cut(s)	543, 753
XagI	CCTNNNNNAGG	1 cut(s)	477
XapI	RAATTY	7 cut(s)	59, 91, 329, 624, 725, 1115, 1337
XceI	RCATGY	2 cut(s)	1994, 2274
XcmI	CCANNNNNNNNNTGG	2 cut(s)	431, 458
XmiI	GTMKAC	3 cut(s)	993, 1169, 1482
XmnI	GAANNNNTTC	2 cut(s)	1008, 2556
XspI	CTAG	3 cut(s)	827, 1104, 2577
ZrmI	AGTACT	1 cut(s)	1565
Zsp2I	ATGCAT	1 cut(s)	241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.