Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0004455

Physical Location & Seq

Genomic Coordinates Forward (+)

55454055 .. 55458494

Gene Structure 4440 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG244400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2577 bp

ATGGAAATCTTTATATACCAGCAAGATCAATATGGTAACCTTGTTCCAGGACTATATGAATTTGATGCTGAAGTTGTTGAGACAGAGACGAATTTGTCAATACCTGTTCCAGATTTGCACTTTGAAGAAGTGGCGGCTGGAATTCAGCTGTTTTCTTTTACTAATCTGGAACCAGGCAACTTTTTGCTGACTATATCAGATATGAAGCATGATAAAAGCATCTCTAATATGCCATATGCTTATACCGTCTTTGTTGGCTATTGCAATGGGATAAACAGTGTTGTTAATGGAACTGGTTTAAATAGCTCCACGGCAGGAGAAAGATCAGAATTCTCAGTTTATTTGAATGATGCTTATCAAAATCCCTCTCCAGTTGAAATCGAAAGGCTTGAAATACAAATTGTAAGGGAAATTGACTCCTATCATGTGCAGTCGAGCATATTTCCTATCCAGATCATCAATGGGAGTGGACCTGCTCAAGGAATAAGATATGGTACAACCAGCCAAATAGAATTTGCTCCTTCACCATCTATGACCTTGACAAATTCTACTGTTGGGAACTTCAGCACTCTGGCAACTGCTCTCAATGTGGTATATACACCTGACAAGAGTGGGATTTATAAAATTTTTGTGTTTTGTGGAAATATTTTGCTGAATGGAGGCCGTCCCTTTGACAAGGAAGTTATAGCAGGGAGTGTTGATCCATCATGCTCAACAGTTGTCAAATTTTCTCCCAAGGTGCCAAGGACGATTAATAATGAAGTAGTAGTGAAGCTCGGGGATTCATTTTTCAATCCCGTCTTGTCACATCAATCTGGGCTGAAACTAGAGATTGTTTCTGTAAACAGTTCTGGCTTTTCAAGTTCCTTGTTTGTGGACAATAATGATGGGTCATACACTGTACACTATCTGGCTAAGGAGGTTGGAACTTATGAGATGTGTGCTTCATTTGATGGCAAACGTATTTCTGCCTGCCCCTTTGGGGTCAATGTCTACAGTAGCGAATATTTTCCCAGAGCCGAAAATGATACAATTTCTGTGTGGGAGGATGAGTCAATTGCTTTTGATGTTTTGGCCAATGACTACTTTGCTGGAAATAATGCTAGTGTTGTTGAGTTCTCCAAGCCAGGTCATGGTTCCCTTATTGAGTATGGAAGGCTCCTCCGGTATACACCTTATAAAGACTATTATGGAAATGATTCCTTTGTGTACACAATGTCTGATATAAACGGCAATCTTGCTACTGCTTTTGTAAACATATCTGTTCTCACCATCCCACCACAATTTGTTTCTTTTCCAAGTGAATTGCAAGCTACTGAAGGCGAGATTAATCCAAAATTTGGTGGTTTCTCTGGGTTTATGATAAGATATTCCGACATGATGGAGAACATCTCTGTTAATCTTAGTGCGCAATCTGGGACAGTATTTCTGTCTCCGATGCTAATGCAATTCTGGGAGCCCATTTGGAATGGACTTTCTGTGTACAAAGAGGATGGAGGAGTGAAGGGGTTGATATTACAAGGCAGTGTGGAAGTAATCAATTTTGCCCTTAAGTCAATACAGTACTATGGTAATGAGAACTTCTGTGGCTATGATACTATTCGGCTCACTACGAGGAATAGGAATGGAGTAAATGACTTGCATATTCCAGTTTTTGTGGAACCTATCAATGATCCTCCCTTTATTCATGCACCTGAGTTTATCATTCTGAAAAGTAATGAAAATGGGTCACTGATATATGACACAGAAACAGACAAGTTTGAATTCTATATTGGAGACCCAGATCTTCTTGCGTTCCCTGGTAATGAATCCCTTTTTTCGGTCATCTTCTCTATGGAAGTTAATGATGGATATTTGGTAACCAGCCTACCAGCTGAGCTCATCACTACAATAGAAGTGAAGACTAAGAATAGCTACCAGTGGCTACCACTTCAGACATATGTTACCATCTCAAAGCATTTCACGGTCAAAGCTAAGGGAATCAGATTTTATGCAACAGTTAATGAATGCAATACTGCTATGCAACGGCTGTTTTATCATGGTGGAGGAGAGCAAGATGCCATTTTGACAGTGACATTGAATGACCTGGGAAACTATGGGTGTTATTGTAACTGTTCCGAAAAGATATCAGTGCCCTTACTTGCTGAGGCTTCTGTTAATCTAATAAGAAGAAGGCCGATGAGTTCACTTGTGTCTCGTGGCTTAGGATCAGCTATTGTCATCGAGTCCATTACAGTGTTTTCACTGGGAGCAGCACTAATGTTTTTCACATGCAAATGTGCAACTATTCTTGTAAATGAAAGAAGAAACAGAGCTTCCAGGACTCCAGCTGACCCAGCAAATGCACATAGTTTCGGCAAAGGAACTTGGAGTAAAAGTTTTCCAGAGAACGCAACTCACCTTACTGCGTATTGTTCAAGTCCCCTGCTTGGCAGCCAACGTCCCAACTTTCGACAACGGTCCTGCCGTCAATCTAGAAATGGGGAATCAAGCAAGGCAACATATAAGGCTACTGGTGAACGCATTCAGCAGAATCCTGCGACTAGTTTTAAGCCCTTTTGTGTTGAGAAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009987 GO:0016020 GO:0016021 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0044425 GO:0044459 GO:0044464 GO:0044703 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Filamin

Protein Analysis

858

Amino Acids

94.9

Weight (kDa)

5.02

Isoelectric Point (pI)

39.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Filamin	PF00630	275 - 327	3.2e-06	Filamin/ABP280 repeat
Big_9	PF17963	338 - 420	1.6e-13	Bacterial Ig domain
Cadherin_5	PF17892	338 - 422	9e-06	Cadherin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004455)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150 AT5G49150
fragaria_vesca	FvH4_4g18570
prunus_persica	Prupe.1G132400_v2.0.a1 Prupe.1G132400_v2.0.a1 Prupe.1G132400_v2.0.a1 Prupe.1G132400_v2.0.a1
pyrus_communis	pycom16g14000 pycom16g14010
rosa_chinensis	RchiOBHm_Chr4g0423171
rosa_laevigata	RLG00000007579
rosa_multiflora	Rmu_sc0000744.1_g000001 Rmu_sc0008829.1_g000002 Rmu_sc0020601.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365330
rosa_rugosa	Rorug04G0184800 Rorug04G0184900
rosa_samantha	Rh4AG244300 Rh4AG244400 Rh4BG249500 Rh4BG249600 Rh4CG258600 Rh4CG258700 Rh4DG244300 Rh4DG244400
rosa_wichuraiana	Rw4G021050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	621, 1179
AasI	GACNNNNNNGTC	1 cut(s)	94
Acc16I	TGCGCA	1 cut(s)	1410
Acc36I	ACCTGC	1 cut(s)	481
AccB1I	GGYRCC	1 cut(s)	739
AccB7I	CCANNNNNTGG	2 cut(s)	1133, 1340
AccI	GTMKAC	2 cut(s)	993, 1169
AciI	CCGC	1 cut(s)	134
AclWI	GGATC	3 cut(s)	695, 1667, 2215
AcoI	YGGCCR	1 cut(s)	1074
AcuI	CTGAAG	4 cut(s)	90, 547, 1338, 1916
AfaI	GTAC	5 cut(s)	496, 903, 1211, 1484, 1565
AfiI	CCNNNNNNNGG	6 cut(s)	479, 982, 1133, 1340, 1819, 2429
AflII	CTTAAG	1 cut(s)	1550
AgsI	TTSAA	9 cut(s)	125, 346, 377, 392, 793, 861, 1763, 2080, 2418
AhlI	ACTAGT	1 cut(s)	2543
AjnI	CCWGG	6 cut(s)	46, 172, 1126, 1798, 2085, 2318
AjuI	GAANNNNNNNTTGG	2 cut(s)	1755, 1787
Alw21I	GWGCWC	1 cut(s)	1881
Alw26I	GTCTC	5 cut(s)	74, 80, 1437, 1770, 2199
AlwI	GGATC	3 cut(s)	695, 1667, 2215
Ama87I	CYCGRG	1 cut(s)	776
AoxI	GGCC	3 cut(s)	661, 1074, 2174
ApeKI	GCWGC	2 cut(s)	2252, 2433
AseI	ATTAAT	2 cut(s)	753, 1329
Asp700I	GAANNNNTTC	3 cut(s)	1008, 1198, 2523
AspLEI	GCGC	1 cut(s)	1411
AspS9I	GGNCC	2 cut(s)	470, 2460
AsuHPI	GGTGA	4 cut(s)	516, 1261, 2390, 2528
AvaI	CYCGRG	1 cut(s)	776
AvaII	GGWCC	2 cut(s)	470, 2460
BaeGI	GKGCMC	1 cut(s)	2136
BalI	TGGCCA	1 cut(s)	1076
BanI	GGYRCC	1 cut(s)	739
BanII	GRGCYC	2 cut(s)	1461, 1881
BauI	CACGAG	1 cut(s)	2196
BbsI	GAAGAC	1 cut(s)	1907
Bbv12I	GWGCWC	1 cut(s)	1881
BbvCI	CCTCAGC	1 cut(s)	2145
BbvI	GCAGC	2 cut(s)	2264, 2445
BccI	CCATC	9 cut(s)	535, 712, 881, 947, 1280, 1375, 1487, 1841, 1955
BceAI	ACGGC	5 cut(s)	327, 648, 1246, 2042, 2451
BcgI	CGANNNNNNTGC	2 cut(s)	1426, 1460
BciT130I	CCWGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
BcoDI	GTCTC	5 cut(s)	74, 80, 1437, 1770, 2199
BcuI	ACTAGT	1 cut(s)	2543
BfaI	CTAG	4 cut(s)	827, 1104, 2475, 2544
BfmI	CTRYAG	1 cut(s)	994
BfrI	CTTAAG	1 cut(s)	1550
BfuAI	ACCTGC	1 cut(s)	481
BglII	AGATCT	1 cut(s)	1783
BisI	GCNGC	3 cut(s)	135, 2253, 2434
BlpI	GCTNAGC	1 cut(s)	1875
BlsI	GCNGC	3 cut(s)	136, 2254, 2435
BmcAI	AGTACT	1 cut(s)	1565
Bme1390I	CCNGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
Bme18I	GGWCC	2 cut(s)	470, 2460
BmeT110I	CYCGRG	1 cut(s)	776
BmgT120I	GGNCC	2 cut(s)	470, 2460
BmiI	GGNNCC	6 cut(s)	171, 741, 1138, 1160, 1458, 1662
BmrFI	CCNGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
BmrI	ACTGGG	1 cut(s)	2255
BmsI	GCATC	5 cut(s)	55, 228, 340, 1428, 2047
BmuI	ACTGGG	1 cut(s)	2255
BoxI	GACNNNNGTC	1 cut(s)	2458
BpiI	GAAGAC	1 cut(s)	1907
BplI	GAGNNNNNCTC	2 cut(s)	1376, 1408
BpmI	CTGGAG	2 cut(s)	354, 2310
Bpu10I	CCTNAGC	4 cut(s)	915, 1974, 2145, 2203
Bpu1102I	GCTNAGC	1 cut(s)	1875
BpuEI	CTTGAG	1 cut(s)	462
BsaBI	GATNNNNATC	1 cut(s)	354
BsaI	GGTCTC	1 cut(s)	1770
BsaJI	CCNNGG	5 cut(s)	309, 735, 743, 1798, 2086
BsaWI	WCCGGW	1 cut(s)	1164
BsaXI	ACNNNNNCTCC	4 cut(s)	1037, 1067, 2362, 2392
Bsc4I	CCNNNNNNNGG	6 cut(s)	479, 982, 1133, 1340, 1819, 2429
Bse1I	ACTGG	6 cut(s)	298, 371, 1649, 1918, 2250, 2518
Bse3DI	GCAATG	1 cut(s)	271
Bse8I	GATNNNNATC	1 cut(s)	354
BseBI	CCWGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
BseDI	CCNNGG	5 cut(s)	309, 735, 743, 1798, 2086
BseGI	GGATG	3 cut(s)	1054, 1272, 1498
BseJI	GATNNNNATC	1 cut(s)	354
BseLI	CCNNNNNNNGG	6 cut(s)	479, 982, 1133, 1340, 1819, 2429
BseMI	GCAATG	1 cut(s)	271
BseMII	CTCAG	4 cut(s)	348, 1686, 1866, 2136
BseNI	ACTGG	6 cut(s)	298, 371, 1649, 1918, 2250, 2518
BseRI	GAGGAG	3 cut(s)	1151, 1512, 2061
BseSI	GKGCMC	1 cut(s)	2136
BseXI	GCAGC	2 cut(s)	2264, 2445
BseYI	CCCAGC	1 cut(s)	2335
BsgI	GTGCAG	1 cut(s)	449
BshFI	GGCC	3 cut(s)	663, 1076, 2176
BshNI	GGYRCC	1 cut(s)	739
BsiHKAI	GWGCWC	1 cut(s)	1881
BsiHKCI	CYCGRG	1 cut(s)	776
BsiSI	CCGG	1 cut(s)	1165
BslFI	GGGAC	4 cut(s)	651, 1432, 2406, 2427
BslI	CCNNNNNNNGG	6 cut(s)	479, 982, 1133, 1340, 1819, 2429
BsmAI	GTCTC	5 cut(s)	74, 80, 1437, 1770, 2199
BsmBI	CGTCTC	1 cut(s)	80
BsmFI	GGGAC	4 cut(s)	651, 1432, 2406, 2427
BsmI	GAATGC	2 cut(s)	2012, 2523
BsnI	GGCC	3 cut(s)	663, 1076, 2176
Bso31I	GGTCTC	1 cut(s)	1770
BsoBI	CYCGRG	1 cut(s)	776
Bsp1286I	GDGCHC	3 cut(s)	1461, 1881, 2136
Bsp1407I	TGTACA	3 cut(s)	901, 1209, 1482
Bsp143I	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
Bsp1720I	GCTNAGC	1 cut(s)	1875
BspACI	CCGC	1 cut(s)	134
BspANI	GGCC	3 cut(s)	663, 1076, 2176
BspCNI	CTCAG	4 cut(s)	347, 1687, 1867, 2137
BspLI	GGNNCC	6 cut(s)	171, 741, 1138, 1160, 1458, 1662
BspMI	ACCTGC	1 cut(s)	481
BspPI	GGATC	3 cut(s)	695, 1667, 2215
BspT107I	GGYRCC	1 cut(s)	739
BspTI	CTTAAG	1 cut(s)	1550
BspTNI	GGTCTC	1 cut(s)	1770
BsrDI	GCAATG	1 cut(s)	271
BsrGI	TGTACA	3 cut(s)	901, 1209, 1482
BsrI	ACTGG	6 cut(s)	298, 371, 1649, 1918, 2250, 2518
BssECI	CCNNGG	5 cut(s)	309, 735, 743, 1798, 2086
BssMI	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
BssNAI	GTATAC	1 cut(s)	1170
BssSI	CACGAG	1 cut(s)	2196
BssT1I	CCWWGG	2 cut(s)	735, 743
Bst1107I	GTATAC	1 cut(s)	1170
Bst2BI	CACGAG	1 cut(s)	2196
Bst2UI	CCWGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
BstAFI	CTTAAG	1 cut(s)	1550
BstAUI	TGTACA	3 cut(s)	901, 1209, 1482
BstC8I	GCNNGC	2 cut(s)	973, 1311
BstDEI	CTNAG	9 cut(s)	334, 915, 1403, 1695, 1875, 1905, 1974, 2145, 2203
BstDSI	CCRYGG	1 cut(s)	309
BstEII	GGTNACC	2 cut(s)	35, 1858
BstENI	CCTNNNNNAGG	1 cut(s)	477
BstF5I	GGATG	3 cut(s)	1054, 1272, 1498
BstHHI	GCGC	1 cut(s)	1411
BstKTI	GATC	7 cut(s)	28, 326, 456, 703, 1675, 1786, 2210
BstMAI	GTCTC	5 cut(s)	74, 80, 1437, 1770, 2199
BstMBI	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
BstMWI	GCNNNNNNNGC	1 cut(s)	2336
BstNI	CCWGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
BstNSI	RCATGY	1 cut(s)	2274
BstPAI	GACNNNNGTC	1 cut(s)	2458
BstPI	GGTNACC	2 cut(s)	35, 1858
BstSCI	CCNGG	6 cut(s)	46, 172, 1126, 1798, 2085, 2318
BstSFI	CTRYAG	1 cut(s)	994
BstSLI	GKGCMC	1 cut(s)	2136
BstV1I	GCAGC	2 cut(s)	2264, 2445
BstV2I	GAAGAC	1 cut(s)	1907
BstX2I	RGATCY	1 cut(s)	1783
BstYI	RGATCY	1 cut(s)	1783
BstZ17I	GTATAC	1 cut(s)	1170
BsuRI	GGCC	3 cut(s)	663, 1076, 2176
BtgI	CCRYGG	1 cut(s)	309
BtsCI	GGATG	3 cut(s)	1054, 1272, 1498
BtsI	GCAGTG	1 cut(s)	1531
BtsIMutI	CAGTG	9 cut(s)	283, 897, 1531, 1730, 1925, 2076, 2136, 2241, 2243
BveI	ACCTGC	1 cut(s)	481
Cac8I	GCNNGC	2 cut(s)	973, 1311
CfoI	GCGC	1 cut(s)	1411
Cfr13I	GGNCC	2 cut(s)	470, 2460
Csp6I	GTAC	5 cut(s)	495, 902, 1210, 1483, 1564
CspCI	CAANNNNNGTGG	2 cut(s)	448, 483
CviAII	CATG	8 cut(s)	209, 425, 708, 1133, 1378, 1688, 2039, 2271
CviQI	GTAC	5 cut(s)	495, 902, 1210, 1483, 1564
DdeI	CTNAG	9 cut(s)	334, 915, 1403, 1695, 1875, 1905, 1974, 2145, 2203
DpnI	GATC	7 cut(s)	27, 325, 455, 702, 1674, 1785, 2209
DpnII	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
DraI	TTTAAA	1 cut(s)	300
DrdI	GACNNNNNNGTC	1 cut(s)	94
DseDI	GACNNNNNNGTC	1 cut(s)	94
EaeI	YGGCCR	1 cut(s)	1074
Ecl136II	GAGCTC	1 cut(s)	1879
Eco130I	CCWWGG	2 cut(s)	735, 743
Eco24I	GRGCYC	2 cut(s)	1461, 1881
Eco31I	GGTCTC	1 cut(s)	1770
Eco32I	GATATC	1 cut(s)	2127
Eco47I	GGWCC	2 cut(s)	470, 2460
Eco53kI	GAGCTC	1 cut(s)	1879
Eco57I	CTGAAG	4 cut(s)	90, 547, 1338, 1916
Eco88I	CYCGRG	1 cut(s)	776
Eco91I	GGTNACC	2 cut(s)	35, 1858
EcoICRI	GAGCTC	1 cut(s)	1879
EcoNI	CCTNNNNNAGG	1 cut(s)	477
EcoO65I	GGTNACC	2 cut(s)	35, 1858
EcoRI	GAATTC	3 cut(s)	141, 329, 1763
EcoRII	CCWGG	6 cut(s)	46, 172, 1126, 1798, 2085, 2318
EcoRV	GATATC	1 cut(s)	2127
EcoT14I	CCWWGG	2 cut(s)	735, 743
EcoT38I	GRGCYC	2 cut(s)	1461, 1881
ErhI	CCWWGG	2 cut(s)	735, 743
Esp3I	CGTCTC	1 cut(s)	80
FaeI	CATG	8 cut(s)	212, 428, 711, 1136, 1381, 1691, 2042, 2274
FaqI	GGGAC	4 cut(s)	651, 1432, 2406, 2427
FatI	CATG	8 cut(s)	208, 424, 707, 1132, 1377, 1687, 2038, 2270
FauNDI	CATATG	2 cut(s)	235, 1939
FblI	GTMKAC	2 cut(s)	993, 1169
Fnu4HI	GCNGC	3 cut(s)	135, 2253, 2434
FokI	GGATG	3 cut(s)	1061, 1259, 1505
FriOI	GRGCYC	2 cut(s)	1461, 1881
Fsp4HI	GCNGC	3 cut(s)	135, 2253, 2434
FspBI	CTAG	4 cut(s)	827, 1104, 2475, 2544
FspI	TGCGCA	1 cut(s)	1410
GlaI	GCGC	1 cut(s)	1410
GluI	GCNGC	3 cut(s)	135, 2253, 2434
GsaI	CCCAGC	1 cut(s)	2339
GsuI	CTGGAG	2 cut(s)	354, 2310
HaeIII	GGCC	3 cut(s)	663, 1076, 2176
HapII	CCGG	1 cut(s)	1165
HhaI	GCGC	1 cut(s)	1411
Hin1II	CATG	8 cut(s)	212, 428, 711, 1136, 1381, 1691, 2042, 2274
Hin6I	GCGC	1 cut(s)	1409
HinP1I	GCGC	1 cut(s)	1409
HpaII	CCGG	1 cut(s)	1165
HphI	GGTGA	4 cut(s)	516, 1261, 2390, 2528
Hpy188I	TCNGA	9 cut(s)	199, 328, 1222, 1375, 1437, 1710, 1935, 1985, 2119
Hpy188III	TCNNGA	5 cut(s)	110, 167, 451, 2384, 2475
HpyAV	CCTTC	6 cut(s)	531, 1149, 1313, 1498, 2166, 2563
HpyCH4IV	ACGT	2 cut(s)	961, 2440
HpyF10VI	GCNNNNNNNGC	1 cut(s)	2336
HpyF3I	CTNAG	9 cut(s)	334, 915, 1403, 1695, 1875, 1905, 1974, 2145, 2203
HpySE526I	ACGT	2 cut(s)	961, 2440
Hsp92II	CATG	8 cut(s)	212, 428, 711, 1136, 1381, 1691, 2042, 2274
HspAI	GCGC	1 cut(s)	1409
Kzo9I	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
LmnI	GCTCC	5 cut(s)	311, 523, 1164, 1456, 2249
Lsp1109I	GCAGC	2 cut(s)	2264, 2445
LweI	GCATC	5 cut(s)	55, 228, 340, 1428, 2047
MaeI	CTAG	4 cut(s)	827, 1104, 2475, 2544
MaeII	ACGT	2 cut(s)	961, 2440
MaeIII	GTNAC	7 cut(s)	35, 804, 1728, 1858, 1942, 2071, 2108
MalI	GATC	7 cut(s)	27, 325, 455, 702, 1674, 1785, 2209
MboI	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
MboII	GAAGA	6 cut(s)	137, 1778, 1819, 1912, 2181, 2316
MfeI	CAATTG	1 cut(s)	1056
MflI	RGATCY	1 cut(s)	1783
MhlI	GDGCHC	3 cut(s)	1461, 1881, 2136
MlsI	TGGCCA	1 cut(s)	1076
MluNI	TGGCCA	1 cut(s)	1076
MlyI	GAGTC	4 cut(s)	410, 1061, 2234, 2317
MmeI	TCCRAC	2 cut(s)	904, 1398
Mox20I	TGGCCA	1 cut(s)	1076
MroXI	GAANNNNTTC	3 cut(s)	1008, 1198, 2523
MscI	TGGCCA	1 cut(s)	1076
MslI	CAYNNNNRTG	2 cut(s)	2234, 2275
Msp20I	TGGCCA	1 cut(s)	1076
MspA1I	CMGCKG	3 cut(s)	148, 1874, 2330
MspCI	CTTAAG	1 cut(s)	1550
MspI	CCGG	1 cut(s)	1165
MspR9I	CCNGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
MunI	CAATTG	1 cut(s)	1056
Mva1269I	GAATGC	2 cut(s)	2012, 2523
MvaI	CCWGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
MwoI	GCNNNNNNNGC	1 cut(s)	2336
NdeI	CATATG	2 cut(s)	235, 1939
NdeII	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
NlaIII	CATG	8 cut(s)	212, 428, 711, 1136, 1381, 1691, 2042, 2274
NlaIV	GGNNCC	6 cut(s)	171, 741, 1138, 1160, 1458, 1662
NmuCI	GTSAC	3 cut(s)	804, 1728, 2071
NsbI	TGCGCA	1 cut(s)	1410
NspI	RCATGY	1 cut(s)	2274
PcsI	WCGNNNNNNNCGW	1 cut(s)	2464
PctI	GAATGC	2 cut(s)	2012, 2523
PdmI	GAANNNNTTC	3 cut(s)	1008, 1198, 2523
PfeI	GAWTC	6 cut(s)	782, 1199, 1808, 1980, 2486, 2533
PflMI	CCANNNNNTGG	2 cut(s)	1133, 1340
PfoI	TCCNGGA	2 cut(s)	46, 2318
PkrI	GCNGC	3 cut(s)	136, 2254, 2435
PleI	GAGTC	4 cut(s)	410, 1060, 2233, 2317
PpsI	GAGTC	4 cut(s)	410, 1060, 2233, 2317
PshAI	GACNNNNGTC	1 cut(s)	2458
PshBI	ATTAAT	2 cut(s)	753, 1329
PsiI	TTATAA	2 cut(s)	621, 1179
Psp124BI	GAGCTC	1 cut(s)	1881
Psp6I	CCWGG	6 cut(s)	46, 172, 1126, 1798, 2085, 2318
PspEI	GGTNACC	2 cut(s)	35, 1858
PspFI	CCCAGC	1 cut(s)	2335
PspGI	CCWGG	6 cut(s)	46, 172, 1126, 1798, 2085, 2318
PspN4I	GGNNCC	6 cut(s)	171, 741, 1138, 1160, 1458, 1662
PspPI	GGNCC	2 cut(s)	470, 2460
PsuI	RGATCY	1 cut(s)	1783
PvuII	CAGCTG	3 cut(s)	148, 1874, 2330
RsaI	GTAC	5 cut(s)	496, 903, 1211, 1484, 1565
RsaNI	GTAC	5 cut(s)	495, 902, 1210, 1483, 1564
RseI	CAYNNNNRTG	2 cut(s)	2234, 2275
SacI	GAGCTC	1 cut(s)	1881
SatI	GCNGC	3 cut(s)	135, 2253, 2434
Sau3AI	GATC	7 cut(s)	25, 323, 453, 700, 1672, 1783, 2207
Sau96I	GGNCC	2 cut(s)	470, 2460
ScaI	AGTACT	1 cut(s)	1565
SchI	GAGTC	4 cut(s)	410, 1061, 2234, 2317
ScrFI	CCNGG	6 cut(s)	48, 174, 1128, 1800, 2087, 2320
SduI	GDGCHC	3 cut(s)	1461, 1881, 2136
SfaNI	GCATC	5 cut(s)	55, 228, 340, 1428, 2047
SfcI	CTRYAG	1 cut(s)	994
SinI	GGWCC	2 cut(s)	470, 2460
SmiMI	CAYNNNNRTG	2 cut(s)	2234, 2275
SmlI	CTYRAG	2 cut(s)	477, 1550
SmoI	CTYRAG	2 cut(s)	477, 1550
SpeI	ACTAGT	1 cut(s)	2543
SsiI	CCGC	1 cut(s)	134
SspI	AATATT	2 cut(s)	646, 1007
SspMI	CTAG	4 cut(s)	827, 1104, 2475, 2544
SstI	GAGCTC	1 cut(s)	1881
StyD4I	CCNGG	6 cut(s)	46, 172, 1126, 1798, 2085, 2318
StyI	CCWWGG	2 cut(s)	735, 743
TaiI	ACGT	2 cut(s)	964, 2443
TaqI	TCGA	4 cut(s)	381, 434, 2223, 2452
TaqII	GACCGA	1 cut(s)	1810
TatI	WGTACW	4 cut(s)	901, 1209, 1482, 1563
TauI	GCSGC	1 cut(s)	137
TfiI	GAWTC	6 cut(s)	782, 1199, 1808, 1980, 2486, 2533
TscAI	CASTG	9 cut(s)	283, 904, 1531, 1737, 1925, 2076, 2136, 2241, 2250
TseFI	GTSAC	3 cut(s)	804, 1728, 2071
TseI	GCWGC	2 cut(s)	2252, 2433
Tsp45I	GTSAC	3 cut(s)	804, 1728, 2071
TspRI	CASTG	9 cut(s)	283, 904, 1531, 1737, 1925, 2076, 2136, 2241, 2250
Van91I	CCANNNNNTGG	2 cut(s)	1133, 1340
Vha464I	CTTAAG	1 cut(s)	1550
VpaK11BI	GGWCC	2 cut(s)	470, 2460
VspI	ATTAAT	2 cut(s)	753, 1329
XagI	CCTNNNNNAGG	1 cut(s)	477
XbaI	TCTAGA	1 cut(s)	2474
XceI	RCATGY	1 cut(s)	2274
XcmI	CCANNNNNNNNNTGG	1 cut(s)	458
XmiI	GTMKAC	2 cut(s)	993, 1169
XmnI	GAANNNNTTC	3 cut(s)	1008, 1198, 2523
XspI	CTAG	4 cut(s)	827, 1104, 2475, 2544
ZrmI	AGTACT	1 cut(s)	1565

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.