FvH4_4g19650

Fip1 motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004701
Physical Location & Seq
Forward (+)
23227396 .. 23234462
7067 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19650.t1

Sequence Viewer

Length: 3555 bp
ATGGCAGATTTGGACGACGATTTCGGAGACATCTACACTGAAGTTGATACCCAGGCCTCTTCAGCCATCAATGGCGTCTCCAACTTCACTCAGTTCTATACTCAACCCGAAGAAGAAGAAGGAGAAGATAGAAATGATAGTAATAACGATAAAAAGAACGGCGGCGAGGGTTTGGTCTCCGATTCTAAGCAACTCAGTTCTGTGTCTGAGGAATTGGGCGGTAAAGTTTCGGGTTCCGAATCGAATGAGGAGTACAGTGATAGTGACAGTGAGGATGATCTCAATATAGTTTTGAATGATGAGGGCTGTAAAGGTATTCCCTTTCCGATTTCGGGTGGTGGGAGCATGAGGATTGATGATTGTGATGATGAGGATGATGATGCTTTTGTGGCTATCAAAGATTCCAAGTATGTATGGACTCAAGGATCGGCTTGTGCAAGCAATGGAAGAGGCAATGAATCTGTGGATTTGGTGTCTTCCATGCCGTTCGGAGGAGACCACTCTCAGATCAGTTGTGTTCGTGATACACCTAATCCAGTGGTGACTCGAAACAGATTTGGTTTTCGACTTCCTTGGTATAGGACAATACTGGACGTGGATATTGAATCGTTTGAGGAGAAGTCATGGAGGTATCCTGGAGTTGATTTAACAGATTACTTCAATTTTGATTTGAATGAGGACAGCTGGAAAGAGTACTGTAAATCCCTGCAACAACTTCGGCGGGTCAAATCCATGCAGTATGGCACGCCTGGCTCTTCAAAATTTTATCAGGCTAATGAAGCTGGATCTGAGCATGGCATATTCGCCCAGGAAATTATGTCTACAGGAGTGAATTATGCTGGATCATGGACATATGCTCCTCCATCATCAACAGTTTCTGATTGCAGACCCATAGGAAACACAATTCAGATAGAAGATAGTATTGTTGAACGCCAACCATCTTTTGATAGCAGGCATCCACGTAGTTGGAATTCTGATGTTGTGATACAGATTTCTGTGCAGGATCCCACCCAGGAAACCCCTAATTCTAGCGCGGAACATGGGCATACAAATAGCAGGGCCCATGAGAAATCTGAAAATGGGGAGTTTGATGCAAATGGAAACCAAGATTATATCTCATATGATGATGATTCATCATTAGGAAGTCCAGAAGTAGACGTGAGAACATTAGATGGGTGTTCCCAGAAGATCAGTGTGTCCCATCCAATGACGATAGACTCTGATGATCACAGAAATAACAAGGTCATTGATGTGGATGGGAATAGGCATAAGGAGGTGAATGGTATCTCATTGGAGGCTATTGAACTTGCTAATAAAGTGACAGAAAGTCCTGACAGGAATACTTCCTCTGCTGATCAATACATGATGGAAACACAATTATCGCTGAGTGATGATGATGATGAAGTTAGTCTCATTTCATCATGTTTTGAATCTGATTCTGAAGCTTCCAGGAACAGTGCTCATTTTGATCCAGAAGATATTCATACTCCTGTAAGGTCTGTGGTGAATTCACAAACTGAACCAAGCAAGTCTGCCACATCTTCTTTTAAGAACTCAAAAGGCAATTGCATCAAGAGAAAAAGAGTTGATATCCAAGATTATTCTATGTGTAGAAGTTCTTCTCAAAAGAAGCATAATCATCAGGGTGGGAGGCTCAACAGTATTGATGGACCAAGTAATCACAGAAACAATGACAATGATATGTCTCTCACCTCAGATACTGAGGACCCAAATGACTGGAATGGCTCAGAGAATCCTCGCGGACAGGAGGAGAGGCTGCGAGGTTCTGGTGATATTAATAGAGCAGATATTTCAGAATATAAAGAACCAAAATTTTCCTGCCATTATGGCATTAGAAATGCTGATAACCATGATCAGAATAAAAGAAAACAGAGAAAGTATTCGAGTAGAAAAGGTAGTCACCAGCTCCAACGGAAACTAGATCGATATGTGAATAGAACTTTTAATGAGAGGCAGAATTGTTGTGAAGATACGGATACAGATTGGAACCACTTTGGAATTACTCGGTTCACTGAGGAGAGGAGTCCTCTTACCAGCAGGGAGTCCAGGGGGTTGCATGCTAGGTATTCCTACACTGTTGGAAAAAGGGATGCTCAGTGGAGAAGGAACAGCAACAAATTGCTGTTTAAGAAAATACCCAATTCTAAGTGGCATGAAGACGATTTTCTGGGAGAAAAAGCTGGGAGATGCACATCATTCACTAGTCGAAAAAGGAATAATTTTGATGATTTTGATGAAAGGAAGTTCCCACCAGTCAGGAGACAACTGAATAACTGGGGACGAAGGGGTGGATATGTGGGCAGTCCCATGTATTTGGATGATTTACACTCTGAGAATTTTGAAGATGTACATTGCAAACCCAAAGAAAATCGACACTTGCGCCATCAGTCTTACAGAGATAAAGCTGATGATGGAAGCTGGAAACACTCCATATCACCAAGAATTGATGTTTTTTGCCCAAGAACAACAGACGAAAGATATTGGAGACATGTGAGAAAAATAGATGTTGAAGAAGCCAATGAGATTGATTGGTTTGATGATCACTATGATGGTTATGAAATTGAAGATAACGTGTATGCTAATGATCATTTAAGGTGGAGAAGATCGAACTGGGGTTCTGAAGTTATGCACTGGACTGAAGATCAGTTTACTGTCAGACATCATGCTGACAAATTGTATTCTGAGAAAGCATCCTGTTCTTATCGAAAATATGTGCGGCATGAAAAATTTCATGCTAAATATGGTCCTTTAAGTGATGGAATGCGTTATGATAATATGCAGCCAGAGCAGCGTAGATTGAAAATGCCCAGAAAAGAAATTGGTGCTAACTTTGTTAATAGAAGCGTTAAGATGTATAGAGGTAAGCATGAGCAGTCAGTGAGGTGCAGGAATTCGATGGACTTGGCTGTTAGGGAACGAAAGATCCTTACAAGATGCTCTAAAGCCAGAAATTTGATGCACAATGGCAGGCCAGAAAATATGGGTGCAGAGATTGGTGGAGAGTGGATGACTTCAGGGATTTCGCAAGCTTGTGAATCTGAGAAGGCGAGGGCAGTCAAAATCACCCAAAATATTATATGGAACCAAAACAACAAGAAAGGGCATGATATTTTTCCAGTTACGGCTCAAAATGCTGATTTGGATATAGAGGAGGGCCAGATTGTAACACAAGAACAGAATACCACACATCCTTTGCAGAGAAAACATGCTTCTGATTATACAGAACCAGCCGACAGCTTGATAAAGGGAGTGTTTGATAGTCGTAATGCCTCTAAAGGGAATAAAGTTGTAGAAGGATATGACAAGCAAAGGATTCTGCAAACAATGGCAAAAATGGAGCAACGTGGGGAGCGCTTTAAGGAGCCAATCACCTTGAAAAAGGAGCCAGACAAGCAGTTGATGCCTGAGGTTGATCCCACGGTTGAAACAGCTGATGAAAAGCAACACAGGCCAGCTCGAAAGAGGCAGTGGGGTGGTAGGTTCTCAAAGAATGACTCTCTAACGTTGGGGATTCCATTGAAAGCTAGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
Fip1

Protein Analysis

1185

Amino Acids

135.7

Weight (kDa)

5.64

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 198 - 240 8.1e-20 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 821, 1155
AccII CGCG 2 cut(s) 1034, 1761
AciI CCGC 6 cut(s) 162, 219, 721, 1034, 1761, 2737
AclI AACGTT 1 cut(s) 3524
AclWI GGATC 8 cut(s) 433, 793, 850, 998, 1011, 1463, 2938, 3428
AcsI RAATTY 8 cut(s) 761, 970, 1507, 1832, 2356, 2747, 2911, 2971
AcuI CTGAAG 6 cut(s) 45, 60, 1461, 2661, 2679, 3018
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 3 cut(s) 254, 695, 2370
AfeI AGCGCT 1 cut(s) 3374
AfiI CCNNNNNNNGG 3 cut(s) 332, 491, 3297
AflIII ACRYGT 2 cut(s) 2509, 2592
AhdI GACNNNNNGTC 1 cut(s) 1326
AhlI ACTAGT 1 cut(s) 2222
AjiI CACGTC 2 cut(s) 595, 1159
AjnI CCWGG 7 cut(s) 51, 634, 748, 807, 1011, 1448, 2066
AloI GAACNNNNNNTCC 2 cut(s) 2928, 2960
Alw21I GWGCWC 1 cut(s) 1462
Alw26I GTCTC 8 cut(s) 21, 82, 181, 489, 1415, 1710, 2275, 2500
AlwI GGATC 8 cut(s) 433, 793, 850, 998, 1011, 1463, 2938, 3428
AlwNI CAGNNNCTG 2 cut(s) 878, 1721
Aor51HI AGCGCT 1 cut(s) 3374
AoxI GGCC 5 cut(s) 54, 1059, 2990, 3175, 3470
ApaI GGGCCC 1 cut(s) 1063
ApeKI GCWGC 3 cut(s) 1777, 2800, 2809
ApoI RAATTY 8 cut(s) 761, 970, 1507, 1832, 2356, 2747, 2911, 2971
ArsI GACNNNNNNTTYG 1 cut(s) 37
AseI ATTAAT 1 cut(s) 1797
Asp700I GAANNNNTTC 4 cut(s) 1479, 1618, 1900, 2748
AspLEI GCGC 3 cut(s) 1034, 2403, 3375
AspS9I GGNCC 6 cut(s) 1059, 1060, 1670, 1726, 2765, 3175
AsuHPI GGTGA 9 cut(s) 553, 1288, 1516, 1702, 1802, 1913, 2448, 3076, 3382
AvaII GGWCC 3 cut(s) 1670, 1726, 2765
AxyI CCTNAGG 1 cut(s) 3426
BaeGI GKGCMC 1 cut(s) 1063
BamHI GGATCC 1 cut(s) 1003
BanII GRGCYC 1 cut(s) 1063
BarI GAAGNNNNNNTAC 2 cut(s) 1603, 1635
BbsI GAAGAC 2 cut(s) 468, 2184
Bbv12I GWGCWC 1 cut(s) 1462
BbvI GCAGC 3 cut(s) 1764, 2812, 2821
BceAI ACGGC 3 cut(s) 175, 469, 3159
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
BciVI GTATCC 2 cut(s) 642, 1990
BclI TGATCA 5 cut(s) 1225, 1354, 1873, 2560, 2605
BcoDI GTCTC 8 cut(s) 21, 82, 181, 489, 1415, 1710, 2275, 2500
BcuI ACTAGT 1 cut(s) 2222
BfaI CTAG 5 cut(s) 1029, 1940, 2082, 2223, 3546
BfmI CTRYAG 1 cut(s) 822
BfoI RGCGCY 1 cut(s) 3376
BfuI GTATCC 2 cut(s) 642, 1990
BglI GCCNNNNNGGC 1 cut(s) 1848
BisI GCNGC 5 cut(s) 163, 1778, 2738, 2801, 2810
BlsI GCNGC 5 cut(s) 164, 1779, 2739, 2802, 2811
BmcAI AGTACT 1 cut(s) 695
Bme1390I CCNGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
Bme18I GGWCC 3 cut(s) 1670, 1726, 2765
BmeRI GACNNNNNGTC 1 cut(s) 1326
BmgBI CACGTC 2 cut(s) 595, 1159
BmgT120I GGNCC 6 cut(s) 1059, 1060, 1670, 1726, 2765, 3175
BmiI GGNNCC 8 cut(s) 235, 1005, 1061, 1728, 2009, 3104, 3384, 3405
BmrFI CCNGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
BmrI ACTGGG 2 cut(s) 2305, 2641
BmuI ACTGGG 2 cut(s) 2305, 2641
BpiI GAAGAC 2 cut(s) 468, 2184
BplI GAGNNNNNCTC 2 cut(s) 2032, 2064
BpmI CTGGAG 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 1945
BsaAI YACGTR 1 cut(s) 962
BsaBI GATNNNNATC 1 cut(s) 2212
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 2 cut(s) 181, 489
BsaJI CCNNGG 6 cut(s) 51, 572, 807, 1011, 2067, 3438
BsaXI ACNNNNNCTCC 2 cut(s) 841, 871
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 491, 3297
Bse1I ACTGG 8 cut(s) 536, 594, 1742, 2273, 2300, 2636, 2657, 3137
Bse21I CCTNAGG 1 cut(s) 3426
Bse3DI GCAATG 3 cut(s) 448, 460, 2371
Bse8I GATNNNNATC 1 cut(s) 2212
BseBI CCWGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
BseCI ATCGAT 1 cut(s) 1945
BseDI CCNNGG 6 cut(s) 51, 572, 807, 1011, 2067, 3438
BseJI GATNNNNATC 1 cut(s) 2212
BseLI CCNNNNNNNGG 3 cut(s) 332, 491, 3297
BseMI GCAATG 3 cut(s) 448, 460, 2371
BseNI ACTGG 8 cut(s) 536, 594, 1742, 2273, 2300, 2636, 2657, 3137
BseRI GAGGAG 8 cut(s) 263, 507, 629, 849, 1784, 2051, 2056, 3185
BseSI GKGCMC 1 cut(s) 1063
BseXI GCAGC 3 cut(s) 1764, 2812, 2821
BseYI CCCAGC 1 cut(s) 2201
BsgI GTGCAG 3 cut(s) 1019, 2926, 3027
Bsh1236I CGCG 2 cut(s) 1034, 1761
BshFI GGCC 5 cut(s) 56, 1061, 2992, 3177, 3472
BshVI ATCGAT 1 cut(s) 1945
BsiHKAI GWGCWC 1 cut(s) 1462
BslFI GGGAC 3 cut(s) 1183, 2310, 2313
BslI CCNNNNNNNGG 3 cut(s) 332, 491, 3297
BsmAI GTCTC 8 cut(s) 21, 82, 181, 489, 1415, 1710, 2275, 2500
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 3 cut(s) 1183, 2310, 2313
BsmI GAATGC 1 cut(s) 2787
BsnI GGCC 5 cut(s) 56, 1061, 2992, 3177, 3472
Bso31I GGTCTC 2 cut(s) 181, 489
Bsp120I GGGCCC 1 cut(s) 1059
Bsp1286I GDGCHC 2 cut(s) 1063, 1462
Bsp1407I TGTACA 1 cut(s) 2368
BspACI CCGC 6 cut(s) 162, 219, 721, 1034, 1761, 2737
BspANI GGCC 5 cut(s) 56, 1061, 2992, 3177, 3472
BspDI ATCGAT 1 cut(s) 1945
BspFNI CGCG 2 cut(s) 1034, 1761
BspLI GGNNCC 8 cut(s) 235, 1005, 1061, 1728, 2009, 3104, 3384, 3405
BspPI GGATC 8 cut(s) 433, 793, 850, 998, 1011, 1463, 2938, 3428
BspQI GCTCTTC 1 cut(s) 760
BspTNI GGTCTC 2 cut(s) 181, 489
BsrDI GCAATG 3 cut(s) 448, 460, 2371
BsrGI TGTACA 1 cut(s) 2368
BsrI ACTGG 8 cut(s) 536, 594, 1742, 2273, 2300, 2636, 2657, 3137
BssECI CCNNGG 6 cut(s) 51, 572, 807, 1011, 2067, 3438
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 572
Bst2UI CCWGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
Bst4CI ACNGT 9 cut(s) 257, 269, 698, 874, 1457, 1661, 2098, 2674, 3442
Bst6I CTCTTC 3 cut(s) 64, 442, 760
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 3421
BstAUI TGTACA 1 cut(s) 2368
BstBAI YACGTR 1 cut(s) 962
BstC8I GCNNGC 7 cut(s) 439, 746, 953, 2079, 2990, 3048, 3474
BstDSI CCRYGG 1 cut(s) 3438
BstFNI CGCG 2 cut(s) 1034, 1761
BstH2I RGCGCY 1 cut(s) 3376
BstHHI GCGC 3 cut(s) 1034, 2403, 3375
BstMAI GTCTC 8 cut(s) 21, 82, 181, 489, 1415, 1710, 2275, 2500
BstNI CCWGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
BstNSI RCATGY 3 cut(s) 2081, 2513, 3230
BstSCI CCNGG 7 cut(s) 51, 634, 748, 807, 1011, 1448, 2066
BstSFI CTRYAG 1 cut(s) 822
BstSLI GKGCMC 1 cut(s) 1063
BstUI CGCG 2 cut(s) 1034, 1761
BstV1I GCAGC 3 cut(s) 1764, 2812, 2821
BstV2I GAAGAC 2 cut(s) 468, 2184
BstX2I RGATCY 3 cut(s) 785, 1003, 2943
BstXI CCANNNNNNTGG 3 cut(s) 966, 1737, 2335
BstYI RGATCY 3 cut(s) 785, 1003, 2943
Bsu15I ATCGAT 1 cut(s) 1945
Bsu36I CCTNAGG 1 cut(s) 3426
BsuI GTATCC 2 cut(s) 642, 1990
BsuRI GGCC 5 cut(s) 56, 1061, 2992, 3177, 3472
BsuTUI ATCGAT 1 cut(s) 1945
BtgI CCRYGG 1 cut(s) 3438
BtrI CACGTC 2 cut(s) 595, 1159
BtsI GCAGTG 1 cut(s) 3494
Cac8I GCNNGC 7 cut(s) 439, 746, 953, 2079, 2990, 3048, 3474
CaiI CAGNNNCTG 2 cut(s) 878, 1721
CfoI GCGC 3 cut(s) 1034, 2403, 3375
Cfr13I GGNCC 6 cut(s) 1059, 1060, 1670, 1726, 2765, 3175
ClaI ATCGAT 1 cut(s) 1945
CseI GACGC 1 cut(s) 64
Csp6I GTAC 3 cut(s) 253, 694, 2369
CviQI GTAC 3 cut(s) 253, 694, 2369
DriI GACNNNNNGTC 1 cut(s) 1326
Eam1104I CTCTTC 3 cut(s) 64, 442, 760
Eam1105I GACNNNNNGTC 1 cut(s) 1326
EarI CTCTTC 3 cut(s) 64, 442, 760
Eco130I CCWWGG 1 cut(s) 572
Eco147I AGGCCT 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 1063
Eco31I GGTCTC 2 cut(s) 181, 489
Eco32I GATATC 1 cut(s) 1591
Eco47I GGWCC 3 cut(s) 1670, 1726, 2765
Eco47III AGCGCT 1 cut(s) 3374
Eco57I CTGAAG 6 cut(s) 45, 60, 1461, 2661, 2679, 3018
Eco81I CCTNAGG 1 cut(s) 3426
EcoO109I RGGNCCY 2 cut(s) 1059, 1726
EcoRI GAATTC 3 cut(s) 970, 1507, 2911
EcoRII CCWGG 7 cut(s) 51, 634, 748, 807, 1011, 1448, 2066
EcoRV GATATC 1 cut(s) 1591
EcoT14I CCWWGG 1 cut(s) 572
EcoT38I GRGCYC 1 cut(s) 1063
ErhI CCWWGG 1 cut(s) 572
Esp3I CGTCTC 1 cut(s) 82
FaqI GGGAC 3 cut(s) 1183, 2310, 2313
FauI CCCGC 1 cut(s) 714
FauNDI CATATG 2 cut(s) 853, 1120
FbaI TGATCA 5 cut(s) 1225, 1354, 1873, 2560, 2605
FblI GTMKAC 2 cut(s) 821, 1155
Fnu4HI GCNGC 5 cut(s) 163, 1778, 2738, 2801, 2810
FriOI GRGCYC 1 cut(s) 1063
Fsp4HI GCNGC 5 cut(s) 163, 1778, 2738, 2801, 2810
FspBI CTAG 5 cut(s) 1029, 1940, 2082, 2223, 3546
GlaI GCGC 3 cut(s) 1033, 2402, 3374
GluI GCNGC 5 cut(s) 163, 1778, 2738, 2801, 2810
GsaI CCCAGC 1 cut(s) 2205
GsuI CTGGAG 1 cut(s) 657
HaeII RGCGCY 1 cut(s) 3376
HaeIII GGCC 5 cut(s) 56, 1061, 2992, 3177, 3472
HgaI GACGC 1 cut(s) 64
HhaI GCGC 3 cut(s) 1034, 2403, 3375
Hin1I GRCGYC 1 cut(s) 75
Hin6I GCGC 3 cut(s) 1032, 2401, 3373
HinP1I GCGC 3 cut(s) 1032, 2401, 3373
HindIII AAGCTT 2 cut(s) 1443, 3048
HphI GGTGA 9 cut(s) 553, 1288, 1516, 1702, 1802, 1913, 2448, 3076, 3382
Hpy166II GTNNAC 4 cut(s) 822, 1156, 2031, 2670
Hpy188III TCNNGA 6 cut(s) 521, 1148, 1331, 1472, 1573, 2278
Hpy8I GTNNAC 4 cut(s) 822, 1156, 2031, 2670
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 5 cut(s) 113, 2118, 2298, 3058, 3308
HpyCH4III ACNGT 9 cut(s) 257, 269, 698, 874, 1457, 1661, 2098, 2674, 3442
HpyCH4IV ACGT 6 cut(s) 594, 961, 1158, 2592, 3364, 3524
HpySE526I ACGT 6 cut(s) 594, 961, 1158, 2592, 3364, 3524
Hsp92I GRCGYC 1 cut(s) 75
HspAI GCGC 3 cut(s) 1032, 2401, 3373
Ksp22I TGATCA 5 cut(s) 1225, 1354, 1873, 2560, 2605
LguI GCTCTTC 1 cut(s) 760
LmnI GCTCC 7 cut(s) 342, 862, 1932, 3358, 3370, 3382, 3403
Lsp1109I GCAGC 3 cut(s) 1764, 2812, 2821
MaeI CTAG 5 cut(s) 1029, 1940, 2082, 2223, 3546
MaeII ACGT 6 cut(s) 594, 961, 1158, 2592, 3364, 3524
MaeIII GTNAC 6 cut(s) 263, 541, 1318, 1919, 3139, 3184
MfeI CAATTG 1 cut(s) 1564
MflI RGATCY 3 cut(s) 785, 1003, 2943
MhlI GDGCHC 2 cut(s) 1063, 1462
MlyI GAGTC 6 cut(s) 412, 538, 1211, 2053, 2072, 3509
MmeI TCCRAC 4 cut(s) 105, 947, 1954, 2080
MroXI GAANNNNTTC 4 cut(s) 1479, 1618, 1900, 2748
MslI CAYNNNNRTG 3 cut(s) 1250, 1644, 2568
MspA1I CMGCKG 2 cut(s) 684, 3452
MspR9I CCNGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
MunI CAATTG 1 cut(s) 1564
Mva1269I GAATGC 1 cut(s) 2787
MvaI CCWGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
MvnI CGCG 2 cut(s) 1034, 1761
NdeI CATATG 2 cut(s) 853, 1120
NlaIV GGNNCC 8 cut(s) 235, 1005, 1061, 1728, 2009, 3104, 3384, 3405
NmuCI GTSAC 4 cut(s) 263, 541, 1318, 1919
NspI RCATGY 3 cut(s) 2081, 2513, 3230
PaeI GCATGC 1 cut(s) 2081
PceI AGGCCT 1 cut(s) 56
PciI ACATGT 1 cut(s) 2509
PciSI GCTCTTC 1 cut(s) 760
PctI GAATGC 1 cut(s) 2787
PdmI GAANNNNTTC 4 cut(s) 1479, 1618, 1900, 2748
PfoI TCCNGGA 2 cut(s) 634, 1448
PkrI GCNGC 5 cut(s) 164, 1779, 2739, 2802, 2811
PleI GAGTC 6 cut(s) 412, 538, 1211, 2052, 2071, 3509
PpsI GAGTC 6 cut(s) 412, 538, 1211, 2052, 2071, 3509
Ppu21I YACGTR 1 cut(s) 962
PpuMI RGGWCCY 1 cut(s) 1726
PscI ACATGT 1 cut(s) 2509
PshBI ATTAAT 1 cut(s) 1797
Psp1406I AACGTT 1 cut(s) 3524
Psp5II RGGWCCY 1 cut(s) 1726
Psp6I CCWGG 7 cut(s) 51, 634, 748, 807, 1011, 1448, 2066
PspFI CCCAGC 1 cut(s) 2201
PspGI CCWGG 7 cut(s) 51, 634, 748, 807, 1011, 1448, 2066
PspN4I GGNNCC 8 cut(s) 235, 1005, 1061, 1728, 2009, 3104, 3384, 3405
PspOMI GGGCCC 1 cut(s) 1059
PspPI GGNCC 6 cut(s) 1059, 1060, 1670, 1726, 2765, 3175
PspPPI RGGWCCY 1 cut(s) 1726
PstNI CAGNNNCTG 2 cut(s) 878, 1721
PsuI RGATCY 3 cut(s) 785, 1003, 2943
PvuII CAGCTG 2 cut(s) 684, 3452
RsaI GTAC 3 cut(s) 254, 695, 2370
RsaNI GTAC 3 cut(s) 253, 694, 2369
RseI CAYNNNNRTG 3 cut(s) 1250, 1644, 2568
SapI GCTCTTC 1 cut(s) 760
SatI GCNGC 5 cut(s) 163, 1778, 2738, 2801, 2810
Sau96I GGNCC 6 cut(s) 1059, 1060, 1670, 1726, 2765, 3175
ScaI AGTACT 1 cut(s) 695
SchI GAGTC 6 cut(s) 412, 538, 1211, 2053, 2072, 3509
ScrFI CCNGG 7 cut(s) 53, 636, 750, 809, 1013, 1450, 2068
SduI GDGCHC 2 cut(s) 1063, 1462
SfcI CTRYAG 1 cut(s) 822
SinI GGWCC 3 cut(s) 1670, 1726, 2765
SmiMI CAYNNNNRTG 3 cut(s) 1250, 1644, 2568
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
SpeI ACTAGT 1 cut(s) 2222
SphI GCATGC 1 cut(s) 2081
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 6 cut(s) 162, 219, 721, 1034, 1761, 2737
SspI AATATT 1 cut(s) 3094
SspMI CTAG 5 cut(s) 1029, 1940, 2082, 2223, 3546
StuI AGGCCT 1 cut(s) 56
StyD4I CCNGG 7 cut(s) 51, 634, 748, 807, 1011, 1448, 2066
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 9 cut(s) 257, 269, 698, 874, 1457, 1661, 2098, 2674, 3442
TaiI ACGT 6 cut(s) 597, 964, 1161, 2595, 3367, 3527
TatI WGTACW 3 cut(s) 252, 693, 2368
TauI GCSGC 2 cut(s) 165, 2740
TseFI GTSAC 4 cut(s) 263, 541, 1318, 1919
TseI GCWGC 3 cut(s) 1777, 2800, 2809
Tsp45I GTSAC 4 cut(s) 263, 541, 1318, 1919
TspGWI ACGGA 2 cut(s) 1948, 2009
VpaK11BI GGWCC 3 cut(s) 1670, 1726, 2765
VspI ATTAAT 1 cut(s) 1797
XapI RAATTY 8 cut(s) 761, 970, 1507, 1832, 2356, 2747, 2911, 2971
XceI RCATGY 3 cut(s) 2081, 2513, 3230
XmiI GTMKAC 2 cut(s) 821, 1155
XmnI GAANNNNTTC 4 cut(s) 1479, 1618, 1900, 2748
XspI CTAG 5 cut(s) 1029, 1940, 2082, 2223, 3546
ZrmI AGTACT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.