MD16G1154500.v1.1

Fip1 motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004701
Physical Location & Seq
Reverse (-)
12254545 .. 12261281
6737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1154500.v1.1.491

Sequence Viewer

Length: 3459 bp
ATGGAGGATTTTGACGATGATTTCGGGGATTTATATGCCGGCGTCGAAGCGCAGGCCAATTCGGCCATGAATGGCGCACCGGACTTCGCTCACTTTTACACAGAACCAGAAGAAGATGTCAGTGAGGATAATGGAAAGGCGAAAACCACTAGTCCTGGAACCGATTCTGACTGCGAGGAGTTGGGCGAGAAAGTTTCTGCTTTGGAGGAGAACGAGGATTACAGTGGAAGTGATAGCGAGGATGACCTGAAAATAGTGCTGAATGATGAGGACTGTGAGGGCAAAGTGTTTCCAAATGGCGGCCGAGGGCGCGTGAAGAATGATGAGCATGAGGATGAGGATAGTGATGGGTTTCCGGCTACGAAAGAGTACAAGTACATAAGGACTCAAGGACCAACTTCTCTAAGCAATGGAAAGGGCAGTGAGGCTGTGGGTTCAGCGTCTTCCATGTCGGTTAGAGGAGACCATGAAAACAGTTTGTGCTATCAACGCAAGGGTTTGAGCTCTCAGATTAGTTGTATTGGTGATACACCTAATCAAATGGTGCCTCAAAATAGATATGGTTTCAGACTTCCTTGGTACCGGACCATACTTGACGTCAACATTGAATCGTTTGAGGAGAAGCCATGGAGGTATCCTGAGGTTGATGTAACAGATTATTTCAATTTCGGTTTGAAAGAGGACAGTTGGAAACAGTACTGTATCTCCCTGGAACAACTACGGCTAACATCTATGCAGTTTAGGATCCCTGTCTCAAGAGCTTCAAAACTTTATCAGGCTAAAGAAGCTGGATCTGAGTATGATAAATTCGCCCAGGAAACTATTTATAAAGAAACGAACAATGCTGGACCAAGAAAGTTTGCTCCTCCATCTAGAGATTCTGATTCCAGTTTCAGAGAGTTGGAACTGCCAAAAGGAAGAGCAATTCAGATTGAAGATAGTATGGTTGAACGCCAACCATCTTTTGATGGTAGGCATCCACGTAGTTTGGATTCTGATGTTGTGATACAGATTGCGGTGCAGGACGGCACTGAGGAAACCACGAACTCTGGTGAGGAACAGGGCCATACAAATTGTACGGCACATGAGGCATCTGAAAATGTGGAATGTAATGCTAATGGTAACCGAACTATTCCTGCCAAAGAAGATGATTTATCAGTTGGAACTCCAGAAGGAAACACAAGGATAGCAGACAGTTGTTCCCGGAGGATCAGTGCCACTCATCCAATGACAATAGATTCTGATCAAAGAAATGACCAGTATGTTGATCTGGATGGGTACGACTATAGGCAGGGGAATGCTGTCTCATTGGAGACTACAGAAATTACAAATAAAACAAAGGACAATGTTGGTGGGGATACTTTATATGTTGATCAATGCATGATGGAAGCACAATTATCACTTGGTGATGACGATCAACTTAGCCCCACTTCCTTTGAAAGTGATTATGAGGCATCCAAGAATAGTGTTCATTTTGATCCAGAAGATATTAATACTCCTGCCACATCATCTTTTAAGAACCCAAAAAGCAATGGCATCAAGAGAAAAATAGTTGATATCAAAGATTACTCCATGCATAAAAGTCCTGCTCGAAAGGAGCATAAACATCAGCAAAGGAGGCTTGACAGTGTTGTTGAACCAAAGATTCATATTAATGATGATGCGTCCCCCACCTCAGAAATGGACGACCTTTATGACAGAGATAGCTCATTGAATTCTAGCAGACGGAAGAAAATTCTGCCAGATTTTGGCTGTAGTGATGGAGAAGATATTTCAGATTATAAAGAATCAAGGCATTATGGTAGAAGACATGCTGACAACCACGTTCATACTGCTAAAACAAATTATTTGGATAGAAAAGGTAGTCACAATATCCAAGACAAGGTCGGTCCATTTTTCAGAAAAAACTGGAATGAAAGGGAGTATTTTTGTGAAGATAGGGATACAGATTGGCATCGTTTTGGAAGAACACAATTCCCTAACGAGAGGAGTCTTCTCCCGAGCAGGGAGTCCAGAGGCTGGCACTCCAAGTATTCCCCACCCACTGTTAAAGAAAGGGATGCACAATTTAAAAGGAACGCCAACAAACTGCAGTTTAAGAAAATACCCAACCATGGTGGTGGCTGTTTCGGTTATAAACATGAAGATGACTTTGTGGGAGAAAAATTTGGGAGACGTGCTTCATTTACTGACCGAAAAAGGAATACTTTGGATGAATTTGATGAATGGCAGTTTCCTCGACTCAGGAGAGATTTAGGGGGAAGGGATGGATATGTTGACAAACCTGTTTTTGATTGGGATGATTCATGGTCTGGGAAAATTGAAGATGAATATTGCAGACATGGAGAAAATCAATACTTGTCCGATCAGTTGTACAGAGATTTACATGCAGGTGAAGGAAGATGGAATGATTCTCTATCACCAAGAAATGATGTTTCTTACTCAAGAACTGCTGAAAGATATGGGAGACGTGAACGAGAAATATACTCTGCTGAATCCAAAGAGAGTAACTGGTTTGATAATCGTTATGATGCTGATGAAATTGATGATATCATTTATCCGAGTGATCAGTTTAAGTGGAGAAGATCTAACTGGCGATCCTCGGTCCTACACTGGACTGAGGATCAGTTAACTGTCAGGCACCATGGTGACAAATTGTATTCTGAGAGGTCATCCCGTTCATATCAGAAATATGTAAGACATGAAAAATTTCATGCTAAATATGGTTCCTACAATGATGCAATGCATGTTGATATGCTATCAGAGCATGATAGATTAGAATTGAAGAGAAAAGGAAGTAGTGCTCACTGTATTAATAGTAGTTCTAAGATGTATATCGGTAAGCATGAGCGGACAGCCCTGAGGTGCAGAGGTTCGATTGACTTGGTTGTTGGGGAAGGAAAGTCCTCTGTAAGAAGCTACAAACGTGGAACTTTGATTCACAATGGTAGGCAGGAAAATATGGATCCAGAGATTGGTGGGGAGCAGACAACTTTAGCGGATTCTAGTGAATCAAGGACAGTCCAATTTAACACCCGAAAAGTTGGACACAACCAAAACAATGCGAAAGGGCTTGATAAGTTTCCAGTTACAGCTCAAAATGGTGATATGGATGTTGAAGAAGGCCAGATTGTAACAGACGGCCTCAATACAGCACACCTACAGAGAAGACATGCTTCTGAGTATTCAGCAGCAGCTTCCAAAGTGAAAAGGCGAACGTTTGCCGGAGAAAGCGGTTCCAGTGGGAACAAGGTTGCAGAAGGATATGACAACCAAAGGATTCTTGAGACAAAGGCAAAAATGGAGAAACGCGGGGAACGGTTTAAGGAGCCAATCATCCTGAAAAAAGAGCCAGACAAGTTGTCCAAGCCTGAGATTGATCTTGTAGTTGAAACAGCTGAAGCACAGCTACATAGGCCATCCCGAAAGAGGCGATGGGGCGGTATAGAGGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
Fip1

Protein Analysis

1153

Amino Acids

131.81

Weight (kDa)

5.49

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 199 - 241 5.2e-19 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 828, 1782, 2136
AarI CACCTGC 1 cut(s) 2381
AatII GACGTC 1 cut(s) 600
Acc36I ACCTGC 1 cut(s) 2381
Acc65I GGTACC 1 cut(s) 579
AccB1I GGYRCC 3 cut(s) 544, 579, 2640
AccB7I CCANNNNNTGG 3 cut(s) 1748, 2019, 2975
AccBSI CCGCTC 1 cut(s) 2851
AccII CGCG 2 cut(s) 312, 3312
AciI CCGC 7 cut(s) 300, 1016, 2851, 2999, 3234, 3312, 3441
AclI AACGTT 1 cut(s) 3218
AclWI GGATC 9 cut(s) 739, 752, 799, 1217, 1472, 2592, 2631, 2960, 2973
AcoI YGGCCR 2 cut(s) 63, 301
AcsI RAATTY 6 cut(s) 806, 1714, 1734, 2165, 2216, 2708
AcuI CTGAAG 1 cut(s) 3420
AcyI GRCGYC 2 cut(s) 42, 597
AdeI CACNNNGTG 1 cut(s) 1406
AfaI GTAC 7 cut(s) 371, 377, 581, 698, 1078, 1280, 2375
AfiI CCNNNNNNNGG 8 cut(s) 299, 1748, 1882, 2005, 2019, 2114, 2975, 3429
AhdI GACNNNNNGTC 1 cut(s) 3361
AhlI ACTAGT 1 cut(s) 149
AjiI CACGTC 2 cut(s) 2177, 2471
AjnI CCWGG 3 cut(s) 154, 708, 813
AleI CACNNNNGTG 1 cut(s) 2646
AluBI AGCT 9 cut(s) 504, 761, 788, 1707, 2919, 3095, 3197, 3398, 3409
AluI AGCT 9 cut(s) 504, 761, 788, 1707, 2919, 3095, 3197, 3398, 3409
Alw21I GWGCWC 2 cut(s) 506, 2806
Alw26I GTCTC 7 cut(s) 456, 757, 1307, 1309, 2167, 2461, 3281
AlwI GGATC 9 cut(s) 739, 752, 799, 1217, 1472, 2592, 2631, 2960, 2973
AlwNI CAGNNNCTG 1 cut(s) 2019
Ama87I CYCGRG 1 cut(s) 1999
AoxI GGCC 7 cut(s) 54, 63, 301, 1063, 3124, 3142, 3416
ApeKI GCWGC 2 cut(s) 3191, 3194
ApoI RAATTY 6 cut(s) 806, 1714, 1734, 2165, 2216, 2708
ArsI GACNNNNNNTTYG 1 cut(s) 37
AseI ATTAAT 3 cut(s) 1491, 1653, 2814
Asp700I GAANNNNTTC 2 cut(s) 163, 2709
Asp718I GGTACC 1 cut(s) 579
AspLEI GCGC 3 cut(s) 52, 77, 312
AspS9I GGNCC 6 cut(s) 392, 585, 848, 1063, 1889, 2605
AsuC2I CCSGG 1 cut(s) 1204
AsuHPI GGTGA 7 cut(s) 536, 1064, 1418, 2405, 2412, 2660, 3116
AvaI CYCGRG 1 cut(s) 1999
AvaII GGWCC 5 cut(s) 392, 585, 848, 1889, 2605
AxyI CCTNAGG 2 cut(s) 639, 2861
BamHI GGATCC 2 cut(s) 744, 2965
BanI GGYRCC 3 cut(s) 544, 579, 2640
BanII GRGCYC 1 cut(s) 506
BarI GAAGNNNNNNTAC 2 cut(s) 3393, 3425
BbsI GAAGAC 4 cut(s) 435, 1813, 1985, 3175
Bbv12I GWGCWC 2 cut(s) 506, 2806
BbvI GCAGC 2 cut(s) 3203, 3206
BceAI ACGGC 4 cut(s) 737, 1042, 1095, 3157
BcgI CGANNNNNNTGC 2 cut(s) 2219, 2253
BciT130I CCWGG 3 cut(s) 156, 710, 815
BciVI GTATCC 3 cut(s) 645, 1351, 1936
BclI TGATCA 3 cut(s) 1243, 1372, 2566
BcnI CCSGG 1 cut(s) 1204
BcoDI GTCTC 7 cut(s) 456, 757, 1307, 1309, 2167, 2461, 3281
BcuI ACTAGT 1 cut(s) 149
BfaI CTAG 4 cut(s) 150, 873, 1719, 3006
BfmI CTRYAG 5 cut(s) 1285, 1317, 1753, 2090, 3161
BfuAI ACCTGC 1 cut(s) 2381
BfuI GTATCC 3 cut(s) 645, 1351, 1936
BglI GCCNNNNNGGC 1 cut(s) 62
BglII AGATCT 1 cut(s) 2585
BisI GCNGC 3 cut(s) 301, 3192, 3195
BlsI GCNGC 3 cut(s) 302, 3193, 3196
BmcAI AGTACT 1 cut(s) 698
Bme1390I CCNGG 4 cut(s) 156, 710, 815, 1204
Bme18I GGWCC 5 cut(s) 392, 585, 848, 1889, 2605
BmeRI GACNNNNNGTC 1 cut(s) 3361
BmeT110I CYCGRG 1 cut(s) 1999
BmgBI CACGTC 2 cut(s) 2177, 2471
BmgT120I GGNCC 6 cut(s) 392, 585, 848, 1063, 1889, 2605
BmiI GGNNCC 9 cut(s) 160, 546, 581, 746, 2642, 2728, 2967, 3238, 3330
BmrFI CCNGG 4 cut(s) 156, 710, 815, 1204
BmsI GCATC 9 cut(s) 985, 1100, 1463, 1545, 1651, 1963, 2050, 2521, 2728
BpiI GAAGAC 4 cut(s) 435, 1813, 1985, 3175
BpmI CTGGAG 1 cut(s) 1152
BpuEI CTTGAG 4 cut(s) 372, 739, 2428, 3305
BpuMI CCSGG 1 cut(s) 1204
BsaAI YACGTR 1 cut(s) 983
BsaBI GATNNNNATC 4 cut(s) 1242, 1413, 1953, 2834
BsaHI GRCGYC 2 cut(s) 42, 597
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 8 cut(s) 304, 575, 626, 708, 813, 2113, 2601, 2644
BsaWI WCCGGW 2 cut(s) 79, 582
BsaXI ACNNNNNCTCC 4 cut(s) 689, 719, 1198, 1228
Bsc4I CCNNNNNNNGG 8 cut(s) 299, 1748, 1882, 2005, 2019, 2114, 2975, 3429
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 8 cut(s) 888, 1258, 1913, 2516, 2597, 2618, 3086, 3240
Bse21I CCTNAGG 2 cut(s) 639, 2861
Bse3DI GCAATG 3 cut(s) 415, 1537, 2748
Bse8I GATNNNNATC 4 cut(s) 1242, 1413, 1953, 2834
BseBI CCWGG 3 cut(s) 156, 710, 815
BseDI CCNNGG 8 cut(s) 304, 575, 626, 708, 813, 2113, 2601, 2644
BseJI GATNNNNATC 4 cut(s) 1242, 1413, 1953, 2834
BseLI CCNNNNNNNGG 8 cut(s) 299, 1748, 1882, 2005, 2019, 2114, 2975, 3429
BseMI GCAATG 3 cut(s) 415, 1537, 2748
BseNI ACTGG 8 cut(s) 888, 1258, 1913, 2516, 2597, 2618, 3086, 3240
BseRI GAGGAG 6 cut(s) 191, 221, 474, 632, 855, 2002
BseX3I CGGCCG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 3203, 3206
BsgI GTGCAG 2 cut(s) 1040, 2887
Bsh1236I CGCG 2 cut(s) 312, 3312
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 7 cut(s) 56, 65, 303, 1065, 3126, 3144, 3418
BshNI GGYRCC 3 cut(s) 544, 579, 2640
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 2 cut(s) 506, 2806
BsiHKCI CYCGRG 1 cut(s) 1999
BsiSI CCGG 6 cut(s) 39, 80, 356, 583, 1204, 3225
BslFI GGGAC 1 cut(s) 1651
BslI CCNNNNNNNGG 8 cut(s) 299, 1748, 1882, 2005, 2019, 2114, 2975, 3429
BsmAI GTCTC 7 cut(s) 456, 757, 1307, 1309, 2167, 2461, 3281
BsmBI CGTCTC 2 cut(s) 2167, 2461
BsmFI GGGAC 1 cut(s) 1651
BsmI GAATGC 1 cut(s) 1303
BsnI GGCC 7 cut(s) 56, 65, 303, 1065, 3126, 3144, 3418
Bso31I GGTCTC 1 cut(s) 456
BsoBI CYCGRG 1 cut(s) 1999
Bsp1286I GDGCHC 2 cut(s) 506, 2806
Bsp1407I TGTACA 1 cut(s) 2373
Bsp19I CCATGG 3 cut(s) 626, 2113, 2644
BspACI CCGC 7 cut(s) 300, 1016, 2851, 2999, 3234, 3312, 3441
BspANI GGCC 7 cut(s) 56, 65, 303, 1065, 3126, 3144, 3418
BspFNI CGCG 2 cut(s) 312, 3312
BspLI GGNNCC 9 cut(s) 160, 546, 581, 746, 2642, 2728, 2967, 3238, 3330
BspMAI CTGCAG 1 cut(s) 2094
BspMI ACCTGC 1 cut(s) 2381
BspPI GGATC 9 cut(s) 739, 752, 799, 1217, 1472, 2592, 2631, 2960, 2973
BspQI GCTCTTC 1 cut(s) 913
BspT107I GGYRCC 3 cut(s) 544, 579, 2640
BspTNI GGTCTC 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 2851
BsrDI GCAATG 3 cut(s) 415, 1537, 2748
BsrFI RCCGGY 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 2373
BsrI ACTGG 8 cut(s) 888, 1258, 1913, 2516, 2597, 2618, 3086, 3240
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 8 cut(s) 304, 575, 626, 708, 813, 2113, 2601, 2644
BssNI GRCGYC 2 cut(s) 42, 597
BssT1I CCWWGG 4 cut(s) 575, 626, 2113, 2644
Bst2UI CCWGG 3 cut(s) 156, 710, 815
Bst6I CTCTTC 2 cut(s) 913, 2780
BstACI GRCGYC 2 cut(s) 42, 597
BstAUI TGTACA 1 cut(s) 2373
BstBAI YACGTR 1 cut(s) 983
BstC8I GCNNGC 3 cut(s) 40, 54, 2021
BstDSI CCRYGG 3 cut(s) 626, 2113, 2644
BstEII GGTNACC 1 cut(s) 1121
BstFNI CGCG 2 cut(s) 312, 3312
BstHHI GCGC 3 cut(s) 52, 77, 312
BstMAI GTCTC 7 cut(s) 456, 757, 1307, 1309, 2167, 2461, 3281
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 8 cut(s) 62, 309, 489, 785, 1088, 1618, 2764, 3415
BstNI CCWGG 3 cut(s) 156, 710, 815
BstNSI RCATGY 4 cut(s) 1814, 2390, 2750, 3176
BstPI GGTNACC 1 cut(s) 1121
BstSCI CCNGG 4 cut(s) 154, 708, 813, 1202
BstSFI CTRYAG 5 cut(s) 1285, 1317, 1753, 2090, 3161
BstUI CGCG 2 cut(s) 312, 3312
BstV1I GCAGC 2 cut(s) 3203, 3206
BstV2I GAAGAC 4 cut(s) 435, 1813, 1985, 3175
BstX2I RGATCY 4 cut(s) 744, 791, 2585, 2965
BstXI CCANNNNNNTGG 1 cut(s) 2120
BstYI RGATCY 4 cut(s) 744, 791, 2585, 2965
BstZI CGGCCG 1 cut(s) 301
Bsu36I CCTNAGG 2 cut(s) 639, 2861
BsuI GTATCC 3 cut(s) 645, 1351, 1936
BsuRI GGCC 7 cut(s) 56, 65, 303, 1065, 3126, 3144, 3418
BtgI CCRYGG 3 cut(s) 626, 2113, 2644
BtgZI GCGATG 1 cut(s) 3448
BtrI CACGTC 2 cut(s) 2177, 2471
BtsI GCAGTG 1 cut(s) 427
BveI ACCTGC 1 cut(s) 2381
Cac8I GCNNGC 3 cut(s) 40, 54, 2021
CaiI CAGNNNCTG 1 cut(s) 2019
CfoI GCGC 3 cut(s) 52, 77, 312
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 6 cut(s) 392, 585, 848, 1063, 1889, 2605
CseI GACGC 3 cut(s) 31, 429, 1653
Csp6I GTAC 7 cut(s) 370, 376, 580, 697, 1077, 1279, 2374
CspCI CAANNNNNGTGG 8 cut(s) 969, 1004, 1333, 1368, 1417, 1452, 2098, 2133
CviQI GTAC 7 cut(s) 370, 376, 580, 697, 1077, 1279, 2374
DraI TTTAAA 1 cut(s) 2071
DraIII CACNNNGTG 1 cut(s) 1406
DriI GACNNNNNGTC 1 cut(s) 3361
EaeI YGGCCR 2 cut(s) 63, 301
EagI CGGCCG 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 913, 2780
Eam1105I GACNNNNNGTC 1 cut(s) 3361
EarI CTCTTC 2 cut(s) 913, 2780
Ecl136II GAGCTC 1 cut(s) 504
EclXI CGGCCG 1 cut(s) 301
Eco130I CCWWGG 4 cut(s) 575, 626, 2113, 2644
Eco24I GRGCYC 1 cut(s) 506
Eco31I GGTCTC 1 cut(s) 456
Eco32I GATATC 2 cut(s) 1558, 2551
Eco47I GGWCC 5 cut(s) 392, 585, 848, 1889, 2605
Eco52I CGGCCG 1 cut(s) 301
Eco53kI GAGCTC 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 3420
Eco81I CCTNAGG 2 cut(s) 639, 2861
Eco88I CYCGRG 1 cut(s) 1999
Eco91I GGTNACC 1 cut(s) 1121
EcoICRI GAGCTC 1 cut(s) 504
EcoO65I GGTNACC 1 cut(s) 1121
EcoRI GAATTC 1 cut(s) 1714
EcoRII CCWGG 3 cut(s) 154, 708, 813
EcoRV GATATC 2 cut(s) 1558, 2551
EcoT14I CCWWGG 4 cut(s) 575, 626, 2113, 2644
EcoT22I ATGCAT 3 cut(s) 1382, 1578, 2748
EcoT38I GRGCYC 1 cut(s) 506
ErhI CCWWGG 4 cut(s) 575, 626, 2113, 2644
Esp3I CGTCTC 2 cut(s) 2167, 2461
FalI AAGNNNNNCTT 4 cut(s) 2191, 2223, 3160, 3192
FaqI GGGAC 1 cut(s) 1651
FauI CCCGC 1 cut(s) 3305
FbaI TGATCA 3 cut(s) 1243, 1372, 2566
Fnu4HI GCNGC 3 cut(s) 301, 3192, 3195
FriOI GRGCYC 1 cut(s) 506
Fsp4HI GCNGC 3 cut(s) 301, 3192, 3195
FspBI CTAG 4 cut(s) 150, 873, 1719, 3006
GlaI GCGC 3 cut(s) 51, 76, 311
GluI GCNGC 3 cut(s) 301, 3192, 3195
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 7 cut(s) 56, 65, 303, 1065, 3126, 3144, 3418
HapII CCGG 6 cut(s) 39, 80, 356, 583, 1204, 3225
HgaI GACGC 3 cut(s) 31, 429, 1653
HhaI GCGC 3 cut(s) 52, 77, 312
Hin1I GRCGYC 2 cut(s) 42, 597
Hin6I GCGC 3 cut(s) 50, 75, 310
HinP1I GCGC 3 cut(s) 50, 75, 310
HincII GTYRAC 3 cut(s) 601, 2278, 2631
HindII GTYRAC 3 cut(s) 601, 2278, 2631
HpaI GTTAAC 1 cut(s) 2631
HpaII CCGG 6 cut(s) 39, 80, 356, 583, 1204, 3225
HphI GGTGA 7 cut(s) 536, 1064, 1418, 2405, 2412, 2660, 3116
Hpy166II GTNNAC 4 cut(s) 601, 2278, 2474, 2631
Hpy8I GTNNAC 4 cut(s) 601, 2278, 2474, 2631
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 6 cut(s) 1166, 2256, 2390, 2891, 3116, 3254
HpyCH4IV ACGT 7 cut(s) 597, 982, 1824, 2176, 2470, 2926, 3218
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 309, 489, 785, 1088, 1618, 2764, 3415
HpySE526I ACGT 7 cut(s) 597, 982, 1824, 2176, 2470, 2926, 3218
Hsp92I GRCGYC 2 cut(s) 42, 597
HspAI GCGC 3 cut(s) 50, 75, 310
KpnI GGTACC 1 cut(s) 583
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 3 cut(s) 1243, 1372, 2566
KspAI GTTAAC 1 cut(s) 2631
LguI GCTCTTC 1 cut(s) 913
LmnI GCTCC 4 cut(s) 868, 1597, 2983, 3328
Lsp1109I GCAGC 2 cut(s) 3203, 3206
LweI GCATC 9 cut(s) 985, 1100, 1463, 1545, 1651, 1963, 2050, 2521, 2728
MaeI CTAG 4 cut(s) 150, 873, 1719, 3006
MaeII ACGT 7 cut(s) 597, 982, 1824, 2176, 2470, 2926, 3218
MaeIII GTNAC 7 cut(s) 649, 1121, 1865, 2507, 2648, 3088, 3133
MbiI CCGCTC 1 cut(s) 2851
MflI RGATCY 4 cut(s) 744, 791, 2585, 2965
MhlI GDGCHC 2 cut(s) 506, 2806
MlyI GAGTC 4 cut(s) 379, 1999, 2018, 2235
MmeI TCCRAC 4 cut(s) 668, 882, 1141, 3025
Mph1103I ATGCAT 3 cut(s) 1382, 1578, 2748
MroNI GCCGGC 1 cut(s) 38
MroXI GAANNNNTTC 2 cut(s) 163, 2709
MslI CAYNNNNRTG 6 cut(s) 333, 1653, 2118, 2145, 2391, 2646
MspA1I CMGCKG 1 cut(s) 3398
MspI CCGG 6 cut(s) 39, 80, 356, 583, 1204, 3225
MspR9I CCNGG 4 cut(s) 156, 710, 815, 1204
Mva1269I GAATGC 1 cut(s) 1303
MvaI CCWGG 3 cut(s) 156, 710, 815
MvnI CGCG 2 cut(s) 312, 3312
MwoI GCNNNNNNNGC 8 cut(s) 62, 309, 489, 785, 1088, 1618, 2764, 3415
NaeI GCCGGC 1 cut(s) 40
NciI CCSGG 1 cut(s) 1204
NcoI CCATGG 3 cut(s) 626, 2113, 2644
NgoMIV GCCGGC 1 cut(s) 38
NlaIV GGNNCC 9 cut(s) 160, 546, 581, 746, 2642, 2728, 2967, 3238, 3330
NmeAIII GCCGAG 1 cut(s) 329
NmuCI GTSAC 2 cut(s) 1865, 2648
NsiI ATGCAT 3 cut(s) 1382, 1578, 2748
NspI RCATGY 4 cut(s) 1814, 2390, 2750, 3176
OliI CACNNNNGTG 1 cut(s) 2646
PaqCI CACCTGC 1 cut(s) 2381
PciSI GCTCTTC 1 cut(s) 913
PctI GAATGC 1 cut(s) 1303
PdiI GCCGGC 1 cut(s) 40
PdmI GAANNNNTTC 2 cut(s) 163, 2709
PflFI GACNNNGTC 1 cut(s) 1883
PflMI CCANNNNNTGG 3 cut(s) 1748, 2019, 2975
PfoI TCCNGGA 2 cut(s) 154, 1202
PkrI GCNGC 3 cut(s) 302, 3193, 3196
PleI GAGTC 4 cut(s) 379, 1998, 2017, 2235
PpsI GAGTC 4 cut(s) 379, 1998, 2017, 2235
Ppu21I YACGTR 1 cut(s) 983
PshBI ATTAAT 3 cut(s) 1491, 1653, 2814
PsiI TTATAA 3 cut(s) 828, 1782, 2136
Psp124BI GAGCTC 1 cut(s) 506
Psp1406I AACGTT 1 cut(s) 3218
Psp6I CCWGG 3 cut(s) 154, 708, 813
PspEI GGTNACC 1 cut(s) 1121
PspGI CCWGG 3 cut(s) 154, 708, 813
PspN4I GGNNCC 9 cut(s) 160, 546, 581, 746, 2642, 2728, 2967, 3238, 3330
PspPI GGNCC 6 cut(s) 392, 585, 848, 1063, 1889, 2605
PsrI GAACNNNNNNTAC 2 cut(s) 203, 235
PstI CTGCAG 1 cut(s) 2094
PstNI CAGNNNCTG 1 cut(s) 2019
PsuI RGATCY 4 cut(s) 744, 791, 2585, 2965
PsyI GACNNNGTC 1 cut(s) 1883
PvuII CAGCTG 1 cut(s) 3398
RsaI GTAC 7 cut(s) 371, 377, 581, 698, 1078, 1280, 2375
RsaNI GTAC 7 cut(s) 370, 376, 580, 697, 1077, 1279, 2374
RseI CAYNNNNRTG 6 cut(s) 333, 1653, 2118, 2145, 2391, 2646
SacI GAGCTC 1 cut(s) 506
SapI GCTCTTC 1 cut(s) 913
SatI GCNGC 3 cut(s) 301, 3192, 3195
Sau96I GGNCC 6 cut(s) 392, 585, 848, 1063, 1889, 2605
ScaI AGTACT 1 cut(s) 698
SchI GAGTC 4 cut(s) 379, 1999, 2018, 2235
ScrFI CCNGG 4 cut(s) 156, 710, 815, 1204
SduI GDGCHC 2 cut(s) 506, 2806
SfaNI GCATC 9 cut(s) 985, 1100, 1463, 1545, 1651, 1963, 2050, 2521, 2728
SfcI CTRYAG 5 cut(s) 1285, 1317, 1753, 2090, 3161
SfiI GGCCNNNNNGGCC 1 cut(s) 62
SinI GGWCC 5 cut(s) 392, 585, 848, 1889, 2605
SmiMI CAYNNNNRTG 6 cut(s) 333, 1653, 2118, 2145, 2391, 2646
SmlI CTYRAG 4 cut(s) 387, 754, 2443, 3284
SmoI CTYRAG 4 cut(s) 387, 754, 2443, 3284
SpeI ACTAGT 1 cut(s) 149
SsiI CCGC 7 cut(s) 300, 1016, 2851, 2999, 3234, 3312, 3441
SspI AATATT 1 cut(s) 2333
SspMI CTAG 4 cut(s) 150, 873, 1719, 3006
SstI GAGCTC 1 cut(s) 506
StyD4I CCNGG 4 cut(s) 154, 708, 813, 1202
StyI CCWWGG 4 cut(s) 575, 626, 2113, 2644
TaiI ACGT 7 cut(s) 600, 985, 1827, 2179, 2473, 2929, 3221
TaqI TCGA 4 cut(s) 45, 1591, 2239, 2876
TaqII GACCGA 3 cut(s) 1877, 2208, 2593
TatI WGTACW 4 cut(s) 369, 375, 696, 2373
TauI GCSGC 1 cut(s) 303
TseFI GTSAC 2 cut(s) 1865, 2648
TseI GCWGC 2 cut(s) 3191, 3194
Tsp45I GTSAC 2 cut(s) 1865, 2648
TspGWI ACGGA 1 cut(s) 1741
Tth111I GACNNNGTC 1 cut(s) 1883
Van91I CCANNNNNTGG 3 cut(s) 1748, 2019, 2975
VpaK11BI GGWCC 5 cut(s) 392, 585, 848, 1889, 2605
VspI ATTAAT 3 cut(s) 1491, 1653, 2814
XapI RAATTY 6 cut(s) 806, 1714, 1734, 2165, 2216, 2708
XbaI TCTAGA 1 cut(s) 872
XceI RCATGY 4 cut(s) 1814, 2390, 2750, 3176
XcmI CCANNNNNNNNNTGG 1 cut(s) 1678
XmnI GAANNNNTTC 2 cut(s) 163, 2709
XspI CTAG 4 cut(s) 150, 873, 1719, 3006
ZraI GACGTC 1 cut(s) 598
ZrmI AGTACT 1 cut(s) 698
Zsp2I ATGCAT 3 cut(s) 1382, 1578, 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.