FvH4_4g21510

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012310
Physical Location & Seq
Reverse (-)
24568567 .. 24571938
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21510.t1

Sequence Viewer

Length: 969 bp
ATGTCCGCCTTATCCAGAGGCCTCATTTCCCGCCTCCGCCTCCTCTCCCTCAACCCCGCCACCTCTTCTTCTTCTTCTACTACTCTACCCGCTACGACGTCGTATTCCCATTTCTTCCGATGCTTCGCCTCCGAGGCCCTGGCCGACGGCGCCGAGATCGCCGAGGATCGGGGCCGGATCATCGAGGCCAAGCCCGGAGTTATGACCCCGCAGTCGAAGCGGACCGGGGTCATCGCCGTCAAGTGTGGGATGTCGGCGCGCTGGGACAAATGGGGTGCTAGGGTTCCAATCACCGTGCTTTGGGTCGATGATAATATCGTCTCTCAGGTTAAAACCCCCGAGAAAGAAGGAATCACTGCCTTACAGATTGGTTGCGGGCAGAAAAAGGCAAAACATCTGACAAAGCCTGAAGTGGGCCATTTCAGAGCTCAAGGTGTTCCACTTAAGAGAAAACTGAGGGAGTTTCCTGTAACAGAAGATGCGCTTCTCCCTGTTGGTATGTCAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGATGTTACAGGAATTACGAGAGGGCATGGTTTTCAGGGTGTGATGAAAAGGTATGGACACCGTGGAGGGCCAGCAAGTCATGGTGCTTCTAAGTCACACCGAAGTATTGGTTCTACTGGTCAGAGAGATGATGCTGGAAGGGTATTTAAGGCCAAAAAGATGCCCGGGCGCATGGGTGGAAAACAGAGAACGGTTAAGAATGTATGGATCTACAAAATTGACCCTGCAAGGAACTTGATATGGGTGAGGGGCCAGGTCCCAGGTGCTGAAGGAAACTTTGTGTTTATCAAGGATGCTTTTTACAAGAAACCCGATGTGTCGATCTTACCATTTCCAACTTACTTTGCTCCAGAGGATGAAGATCCAAGCAAGCTAGAACCGCTTGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGTGGCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

323

Amino Acids

35.18

Weight (kDa)

9.84

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 172 - 255 3.5e-16 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
AatII GACGTC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 149
AccII CGCG 1 cut(s) 259
AciI CCGC 9 cut(s) 6, 31, 37, 57, 90, 209, 220, 375, 920
AclWI GGATC 5 cut(s) 174, 185, 755, 896, 941
AcoI YGGCCR 1 cut(s) 141
AcuI CTGAAG 2 cut(s) 429, 828
AcyI GRCGYC 2 cut(s) 98, 150
AfiI CCNNNNNNNGG 3 cut(s) 168, 300, 413
AflII CTTAAG 1 cut(s) 443
AjnI CCWGG 4 cut(s) 138, 526, 792, 799
AluBI AGCT 2 cut(s) 428, 913
AluI AGCT 2 cut(s) 428, 913
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 1 cut(s) 325
AlwI GGATC 5 cut(s) 174, 185, 755, 896, 941
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 2 cut(s) 338, 704
AspLEI GCGC 5 cut(s) 152, 259, 261, 484, 711
AspS9I GGNCC 7 cut(s) 136, 172, 222, 415, 608, 790, 796
AsuC2I CCSGG 4 cut(s) 195, 226, 705, 706
AsuHPI GGTGA 2 cut(s) 283, 796
AvaI CYCGRG 2 cut(s) 338, 704
AvaII GGWCC 2 cut(s) 222, 796
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 430
Bbv12I GWGCWC 1 cut(s) 430
BceAI ACGGC 2 cut(s) 163, 221
BciT130I CCWGG 4 cut(s) 140, 528, 794, 801
BcnI CCSGG 4 cut(s) 195, 226, 705, 706
BcoDI GTCTC 1 cut(s) 325
BfaI CTAG 2 cut(s) 279, 914
BfoI RGCGCY 1 cut(s) 153
BfrI CTTAAG 1 cut(s) 443
BglI GCCNNNNNGGC 1 cut(s) 134
Bme1390I CCNGG 8 cut(s) 140, 195, 226, 528, 705, 706, 794, 801
Bme18I GGWCC 2 cut(s) 222, 796
BmeT110I CYCGRG 2 cut(s) 338, 704
BmgT120I GGNCC 7 cut(s) 136, 172, 222, 415, 608, 790, 796
BmiI GGNNCC 5 cut(s) 151, 173, 285, 791, 798
BmrFI CCNGG 8 cut(s) 140, 195, 226, 528, 705, 706, 794, 801
BmsI GCATC 5 cut(s) 110, 469, 661, 690, 823
BoxI GACNNNNGTC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 414
BpuMI CCSGG 4 cut(s) 195, 226, 705, 706
BsaBI GATNNNNATC 1 cut(s) 900
BsaHI GRCGYC 2 cut(s) 98, 150
BsaJI CCNNGG 7 cut(s) 132, 138, 162, 225, 601, 704, 799
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 300, 413
Bse1I ACTGG 1 cut(s) 661
Bse8I GATNNNNATC 1 cut(s) 900
BseBI CCWGG 4 cut(s) 140, 528, 794, 801
BseDI CCNNGG 7 cut(s) 132, 138, 162, 225, 601, 704, 799
BseGI GGATG 3 cut(s) 255, 838, 901
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 3 cut(s) 168, 300, 413
BseMII CTCAG 2 cut(s) 338, 446
BseNI ACTGG 1 cut(s) 661
BsePI GCGCGC 1 cut(s) 257
BseRI GAGGAG 1 cut(s) 32
BseYI CCCAGC 1 cut(s) 261
Bsh1236I CGCG 1 cut(s) 259
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 430
BsiHKCI CYCGRG 2 cut(s) 338, 704
BsiSI CCGG 4 cut(s) 175, 195, 225, 705
BslFI GGGAC 2 cut(s) 278, 782
BslI CCNNNNNNNGG 3 cut(s) 168, 300, 413
BsmAI GTCTC 1 cut(s) 325
BsmBI CGTCTC 1 cut(s) 325
BsmFI GGGAC 2 cut(s) 278, 782
BsoBI CYCGRG 2 cut(s) 338, 704
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
BspACI CCGC 9 cut(s) 6, 31, 37, 57, 90, 209, 220, 375, 920
BspCNI CTCAG 2 cut(s) 337, 447
BspFNI CGCG 1 cut(s) 259
BspLI GGNNCC 5 cut(s) 151, 173, 285, 791, 798
BspPI GGATC 5 cut(s) 174, 185, 755, 896, 941
BspT107I GGYRCC 1 cut(s) 149
BspTI CTTAAG 1 cut(s) 443
BsrI ACTGG 1 cut(s) 661
BssECI CCNNGG 7 cut(s) 132, 138, 162, 225, 601, 704, 799
BssHII GCGCGC 1 cut(s) 257
BssMI GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
BssNI GRCGYC 2 cut(s) 98, 150
Bst2UI CCWGG 4 cut(s) 140, 528, 794, 801
Bst4CI ACNGT 3 cut(s) 295, 602, 733
Bst6I CTCTTC 1 cut(s) 70
BstACI GRCGYC 2 cut(s) 98, 150
BstAFI CTTAAG 1 cut(s) 443
BstC8I GCNNGC 4 cut(s) 259, 377, 612, 911
BstDEI CTNAG 3 cut(s) 324, 455, 630
BstDSI CCRYGG 1 cut(s) 601
BstF5I GGATG 3 cut(s) 255, 838, 901
BstFNI CGCG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 5 cut(s) 152, 259, 261, 484, 711
BstKTI GATC 8 cut(s) 159, 169, 180, 750, 864, 904, 934, 949
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
BstMWI GCNNNNNNNGC 5 cut(s) 134, 149, 158, 217, 919
BstNI CCWGG 4 cut(s) 140, 528, 794, 801
BstPAI GACNNNNGTC 1 cut(s) 227
BstSCI CCNGG 8 cut(s) 138, 193, 224, 526, 703, 704, 792, 799
BstUI CGCG 1 cut(s) 259
BstX2I RGATCY 3 cut(s) 747, 901, 946
BstYI RGATCY 3 cut(s) 747, 901, 946
BtgI CCRYGG 1 cut(s) 601
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 3 cut(s) 255, 838, 901
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 4 cut(s) 259, 377, 612, 911
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 5 cut(s) 152, 259, 261, 484, 711
Cfr13I GGNCC 7 cut(s) 136, 172, 222, 415, 608, 790, 796
Cfr9I CCCGGG 1 cut(s) 704
CpoI CGGWCCG 1 cut(s) 222
CspI CGGWCCG 1 cut(s) 222
CviAII CATG 3 cut(s) 566, 620, 712
DdeI CTNAG 3 cut(s) 324, 455, 630
DinI GGCGCC 1 cut(s) 151
DpnI GATC 8 cut(s) 158, 168, 179, 749, 863, 903, 933, 948
DpnII GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 428
Eco147I AGGCCT 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 222, 796
Eco53kI GAGCTC 1 cut(s) 428
Eco57I CTGAAG 2 cut(s) 429, 828
Eco88I CYCGRG 2 cut(s) 338, 704
EcoICRI GAGCTC 1 cut(s) 428
EcoO109I RGGNCCY 2 cut(s) 136, 796
EcoRII CCWGG 4 cut(s) 138, 526, 792, 799
EcoT38I GRGCYC 1 cut(s) 430
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
Esp3I CGTCTC 1 cut(s) 325
FaeI CATG 3 cut(s) 569, 623, 715
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FaqI GGGAC 2 cut(s) 278, 782
FatI CATG 3 cut(s) 565, 619, 711
FauI CCCGC 5 cut(s) 38, 64, 97, 216, 368
FokI GGATG 3 cut(s) 262, 845, 908
FriOI GRGCYC 1 cut(s) 430
FspBI CTAG 2 cut(s) 279, 914
GlaI GCGC 5 cut(s) 151, 258, 260, 483, 710
GsaI CCCAGC 1 cut(s) 265
GsuI CTGGAG 1 cut(s) 873
HaeII RGCGCY 1 cut(s) 153
HapII CCGG 4 cut(s) 175, 195, 225, 705
HhaI GCGC 5 cut(s) 152, 259, 261, 484, 711
Hin1I GRCGYC 2 cut(s) 98, 150
Hin1II CATG 3 cut(s) 569, 623, 715
Hin6I GCGC 5 cut(s) 150, 257, 259, 482, 709
HinP1I GCGC 5 cut(s) 150, 257, 259, 482, 709
HinfI GANTC 1 cut(s) 351
HpaII CCGG 4 cut(s) 175, 195, 225, 705
HphI GGTGA 2 cut(s) 283, 796
Hpy188I TCNGA 5 cut(s) 119, 133, 399, 425, 663
Hpy188III TCNNGA 2 cut(s) 15, 890
Hpy99I CGWCG 3 cut(s) 100, 103, 149
HpyAV CCTTC 3 cut(s) 341, 672, 803
HpyCH4III ACNGT 3 cut(s) 295, 602, 733
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 1 cut(s) 767
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 149, 158, 217, 919
HpyF3I CTNAG 3 cut(s) 324, 455, 630
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 2 cut(s) 98, 150
Hsp92II CATG 3 cut(s) 569, 623, 715
HspAI GCGC 5 cut(s) 150, 257, 259, 482, 709
KasI GGCGCC 1 cut(s) 149
Kzo9I GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
LmnI GCTCC 1 cut(s) 892
LweI GCATC 5 cut(s) 110, 469, 661, 690, 823
MaeI CTAG 2 cut(s) 279, 914
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 3 cut(s) 469, 544, 633
MalI GATC 8 cut(s) 158, 168, 179, 749, 863, 903, 933, 948
MboI GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
MboII GAAGA 7 cut(s) 57, 60, 63, 66, 106, 488, 911
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 3 cut(s) 747, 901, 946
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 3 cut(s) 504, 552, 756
Mly113I GGCGCC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 899
MseI TTAA 4 cut(s) 330, 444, 687, 735
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 4 cut(s) 175, 195, 225, 705
MspR9I CCNGG 8 cut(s) 140, 195, 226, 528, 705, 706, 794, 801
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 4 cut(s) 140, 528, 794, 801
MvnI CGCG 1 cut(s) 259
MwoI GCNNNNNNNGC 5 cut(s) 134, 149, 158, 217, 919
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 4 cut(s) 195, 226, 705, 706
NdeII GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
NlaIII CATG 3 cut(s) 569, 623, 715
NlaIV GGNNCC 5 cut(s) 151, 173, 285, 791, 798
NmeAIII GCCGAG 2 cut(s) 178, 187
NmuCI GTSAC 1 cut(s) 633
PauI GCGCGC 1 cut(s) 257
PceI AGGCCT 1 cut(s) 21
PfeI GAWTC 1 cut(s) 351
PluTI GGCGCC 1 cut(s) 153
PpuMI RGGWCCY 1 cut(s) 796
PshAI GACNNNNGTC 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 430
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 4 cut(s) 138, 526, 792, 799
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 4 cut(s) 138, 526, 792, 799
PspN4I GGNNCC 5 cut(s) 151, 173, 285, 791, 798
PspPI GGNCC 7 cut(s) 136, 172, 222, 415, 608, 790, 796
PspPPI RGGWCCY 1 cut(s) 796
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 3 cut(s) 747, 901, 946
PteI GCGCGC 1 cut(s) 257
Rsr2I CGGWCCG 1 cut(s) 222
RsrII CGGWCCG 1 cut(s) 222
SacI GAGCTC 1 cut(s) 430
SaqAI TTAA 4 cut(s) 330, 444, 687, 735
Sau3AI GATC 8 cut(s) 156, 166, 177, 747, 861, 901, 931, 946
Sau96I GGNCC 7 cut(s) 136, 172, 222, 415, 608, 790, 796
ScrFI CCNGG 8 cut(s) 140, 195, 226, 528, 705, 706, 794, 801
SduI GDGCHC 1 cut(s) 430
SetI ASST 9 cut(s) 65, 101, 330, 430, 436, 593, 798, 805, 915
SfaNI GCATC 5 cut(s) 110, 469, 661, 690, 823
SfoI GGCGCC 1 cut(s) 151
SinI GGWCC 2 cut(s) 222, 796
SmaI CCCGGG 1 cut(s) 706
SmlI CTYRAG 2 cut(s) 429, 443
SmoI CTYRAG 2 cut(s) 429, 443
SrfI GCCCGGGC 1 cut(s) 706
Sse9I AATT 3 cut(s) 504, 552, 756
SseBI AGGCCT 1 cut(s) 21
SsiI CCGC 9 cut(s) 6, 31, 37, 57, 90, 209, 220, 375, 920
SspDI GGCGCC 1 cut(s) 149
SspMI CTAG 2 cut(s) 279, 914
SstI GAGCTC 1 cut(s) 430
StuI AGGCCT 1 cut(s) 21
StyD4I CCNGG 8 cut(s) 138, 193, 224, 526, 703, 704, 792, 799
TaaI ACNGT 3 cut(s) 295, 602, 733
TaiI ACGT 1 cut(s) 101
TaqI TCGA 4 cut(s) 183, 215, 306, 860
TasI AATT 3 cut(s) 504, 552, 756
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 4 cut(s) 330, 444, 687, 735
Tru9I TTAA 4 cut(s) 330, 444, 687, 735
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 633
Tsp45I GTSAC 1 cut(s) 633
TspDTI ATGAA 2 cut(s) 599, 912
TspMI CCCGGG 1 cut(s) 704
TspRI CASTG 1 cut(s) 361
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 2 cut(s) 222, 796
XmaI CCCGGG 1 cut(s) 704
XspI CTAG 2 cut(s) 279, 914
ZraI GACGTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.