Rh4CG296000

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012310
Physical Location & Seq
Reverse (-)
55520200 .. 55523266
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG296000.1

Sequence Viewer

Length: 990 bp
ATGTCCGCCTTATCGAGAGGCCTCATTTCCCGCCTCCGCCTCCTCTCCCTCAACCCCGCCACTTCTTCTTCCTCCTCTTCTACTTGTACCGCTATACCCGCCGCTCACGCTACGACGACGTCGTATTCTCATTTCTTCCGATGCTTCAGCTCCGAGGCCTTGGTGGACGGGGCCGAGATCGCCGAAGATCGCAGCCGGGTTATCGAGGCCAAGCCCGGAGTTATGACCCCCCAATCGAAGCGGACCGGAGTCATCGCCGTCAAGTGTGGCATGTCGGCACGCTGGGACAAATGGGGTGCTAGGGTTCCGATCACCGTGCTTTGGGTCGATGATAATATCGTCTCTCAGGTCAAAACCCCAGAGAAAGAAGGAATCTCTGCCCTACAGATTGGGTGCGGGCAGACAAAAGCCAAACATCTGACAAAGCCTGAAGTGGGCCATTTCAGAGCTCAGGGTGTTCCACTTAAGAGGAAACTGCGGGAGTTTCCTGTAACAGAAGATGCGCTTCTCCCTGTTGGTATGTCAATCGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGATGTTACAGGAATCACGAGAGGGAAAGGTTTTCAGGGTGGGATGAAAAAGTATGGTTTTAGAGGAATGCCAGCAAGTCATGGTGCTTCTAAGTCGCACCGAAGTATTGGTTCTACAGGTCAGAGAGATGACGCTGGAAGGGTCTTTAAAGGCAGAAAGATGCCCGGGCGCATGGGTGGAAAACAGAGAACGGTGAAAAATGTATGGATCTACAAAATTGATCCCGCAAGGAACTTGATATGGGTGAGAGGCCAGGTCCCAGGTGCTGAAGGGAACTTTGTGTTTATCAAGGATGCTTTCTACAAGAAACCCGATGTCTCGATGCTACCATTTCCAACTTACTTTGCTCCAGAGGATGAAGATCCAAGCAAGTTAGAACCGCTGGTTGCTGATCTTGGAGAAGTAGATCCATTTATGTCGGCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

329

Amino Acids

35.87

Weight (kDa)

9.79

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 179 - 262 5.8e-16 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 122
AccBSI CCGCTC 1 cut(s) 104
AclWI GGATC 4 cut(s) 776, 776, 917, 962
AcuI CTGAAG 3 cut(s) 130, 450, 849
AcyI GRCGYC 1 cut(s) 119
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 2 cut(s) 321, 434
AflII CTTAAG 1 cut(s) 464
AjnI CCWGG 3 cut(s) 547, 813, 820
AluBI AGCT 2 cut(s) 150, 449
AluI AGCT 2 cut(s) 150, 449
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 2 cut(s) 346, 883
AlwI GGATC 4 cut(s) 776, 776, 917, 962
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 1 cut(s) 725
AoxI GGCC 7 cut(s) 19, 156, 171, 207, 436, 550, 811
ApeKI GCWGC 1 cut(s) 192
AspLEI GCGC 2 cut(s) 505, 732
AspS9I GGNCC 4 cut(s) 171, 243, 436, 817
AsuC2I CCSGG 4 cut(s) 197, 216, 726, 727
AsuHPI GGTGA 3 cut(s) 304, 766, 817
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 2 cut(s) 243, 817
BanII GRGCYC 1 cut(s) 451
BauI CACGAG 1 cut(s) 577
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 242
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 3 cut(s) 549, 815, 822
BcnI CCSGG 4 cut(s) 197, 216, 726, 727
BcoDI GTCTC 2 cut(s) 346, 883
BfaI CTAG 1 cut(s) 300
BfmI CTRYAG 2 cut(s) 383, 675
BfrI CTTAAG 1 cut(s) 464
BisI GCNGC 2 cut(s) 102, 193
BlsI GCNGC 2 cut(s) 103, 194
Bme1390I CCNGG 7 cut(s) 197, 216, 549, 726, 727, 815, 822
Bme18I GGWCC 2 cut(s) 243, 817
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 4 cut(s) 171, 243, 436, 817
BmiI GGNNCC 3 cut(s) 172, 306, 819
BmrFI CCNGG 7 cut(s) 197, 216, 549, 726, 727, 815, 822
BmsI GCATC 5 cut(s) 131, 490, 711, 844, 873
BoxI GACNNNNGTC 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 894
Bpu10I CCTNAGC 1 cut(s) 450
BpuMI CCSGG 4 cut(s) 197, 216, 726, 727
BsaBI GATNNNNATC 1 cut(s) 921
BsaHI GRCGYC 1 cut(s) 119
BsaJI CCNNGG 4 cut(s) 153, 159, 725, 820
BsaWI WCCGGW 1 cut(s) 245
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 434
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 3 cut(s) 549, 815, 822
BseDI CCNNGG 4 cut(s) 153, 159, 725, 820
BseGI GGATG 3 cut(s) 609, 859, 922
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 2 cut(s) 321, 434
BseMII CTCAG 2 cut(s) 359, 464
BseRI GAGGAG 2 cut(s) 32, 64
BseXI GCAGC 1 cut(s) 204
BseYI CCCAGC 1 cut(s) 282
BshFI GGCC 7 cut(s) 21, 158, 173, 209, 438, 552, 813
BsiHKAI GWGCWC 1 cut(s) 451
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 4 cut(s) 196, 216, 246, 726
BslFI GGGAC 2 cut(s) 299, 803
BslI CCNNNNNNNGG 2 cut(s) 321, 434
BsmAI GTCTC 2 cut(s) 346, 883
BsmBI CGTCTC 1 cut(s) 346
BsmFI GGGAC 2 cut(s) 299, 803
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 7 cut(s) 21, 158, 173, 209, 438, 552, 813
BsoBI CYCGRG 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
BspANI GGCC 7 cut(s) 21, 158, 173, 209, 438, 552, 813
BspCNI CTCAG 2 cut(s) 358, 463
BspLI GGNNCC 3 cut(s) 172, 306, 819
BspPI GGATC 4 cut(s) 776, 776, 917, 962
BspTI CTTAAG 1 cut(s) 464
BsrBI CCGCTC 1 cut(s) 104
BssECI CCNNGG 4 cut(s) 153, 159, 725, 820
BssMI GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
BssNI GRCGYC 1 cut(s) 119
BssSI CACGAG 1 cut(s) 577
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 577
Bst2UI CCWGG 3 cut(s) 549, 815, 822
Bst4CI ACNGT 2 cut(s) 316, 754
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 119
BstAFI CTTAAG 1 cut(s) 464
BstC8I GCNNGC 3 cut(s) 280, 398, 633
BstDEI CTNAG 3 cut(s) 345, 450, 651
BstF5I GGATG 3 cut(s) 609, 859, 922
BstHHI GCGC 2 cut(s) 505, 732
BstKTI GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
BstMAI GTCTC 2 cut(s) 346, 883
BstMBI GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
BstMWI GCNNNNNNNGC 3 cut(s) 98, 107, 179
BstNI CCWGG 3 cut(s) 549, 815, 822
BstNSI RCATGY 1 cut(s) 274
BstPAI GACNNNNGTC 1 cut(s) 248
BstSCI CCNGG 7 cut(s) 195, 214, 547, 724, 725, 813, 820
BstSFI CTRYAG 2 cut(s) 383, 675
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 3 cut(s) 768, 922, 967
BstYI RGATCY 3 cut(s) 768, 922, 967
BsuRI GGCC 7 cut(s) 21, 158, 173, 209, 438, 552, 813
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 609, 859, 922
Cac8I GCNNGC 3 cut(s) 280, 398, 633
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 2 cut(s) 505, 732
Cfr13I GGNCC 4 cut(s) 171, 243, 436, 817
Cfr9I CCCGGG 1 cut(s) 725
CpoI CGGWCCG 1 cut(s) 243
CseI GACGC 1 cut(s) 701
Csp6I GTAC 1 cut(s) 87
CspI CGGWCCG 1 cut(s) 243
CviAII CATG 3 cut(s) 271, 641, 733
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 3 cut(s) 345, 450, 651
DpnI GATC 8 cut(s) 179, 189, 311, 770, 783, 924, 954, 969
DpnII GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
DraI TTTAAA 1 cut(s) 709
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 2 cut(s) 21, 158
Eco24I GRGCYC 1 cut(s) 451
Eco47I GGWCC 2 cut(s) 243, 817
Eco53kI GAGCTC 1 cut(s) 449
Eco57I CTGAAG 3 cut(s) 130, 450, 849
Eco88I CYCGRG 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 3 cut(s) 547, 813, 820
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 3 cut(s) 274, 644, 736
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FaqI GGGAC 2 cut(s) 299, 803
FatI CATG 3 cut(s) 270, 640, 732
FauI CCCGC 6 cut(s) 38, 64, 106, 389, 471, 793
Fnu4HI GCNGC 2 cut(s) 102, 193
FokI GGATG 3 cut(s) 616, 866, 929
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 2 cut(s) 102, 193
FspBI CTAG 1 cut(s) 300
GlaI GCGC 2 cut(s) 504, 731
GluI GCNGC 2 cut(s) 102, 193
GsaI CCCAGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 894
HaeIII GGCC 7 cut(s) 21, 158, 173, 209, 438, 552, 813
HapII CCGG 4 cut(s) 196, 216, 246, 726
HgaI GACGC 1 cut(s) 701
HhaI GCGC 2 cut(s) 505, 732
Hin1I GRCGYC 1 cut(s) 119
Hin1II CATG 3 cut(s) 274, 644, 736
Hin6I GCGC 2 cut(s) 503, 730
HinP1I GCGC 2 cut(s) 503, 730
HinfI GANTC 3 cut(s) 249, 372, 573
HpaII CCGG 4 cut(s) 196, 216, 246, 726
HphI GGTGA 3 cut(s) 304, 766, 817
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 6 cut(s) 140, 154, 309, 420, 446, 684
Hpy188III TCNNGA 4 cut(s) 15, 577, 880, 911
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 3 cut(s) 118, 121, 124
HpyAV CCTTC 3 cut(s) 362, 693, 824
HpyCH4III ACNGT 2 cut(s) 316, 754
HpyCH4IV ACGT 1 cut(s) 119
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 107, 179
HpyF3I CTNAG 3 cut(s) 345, 450, 651
HpySE526I ACGT 1 cut(s) 119
Hsp92I GRCGYC 1 cut(s) 119
Hsp92II CATG 3 cut(s) 274, 644, 736
HspAI GCGC 2 cut(s) 503, 730
Kzo9I GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
LmnI GCTCC 2 cut(s) 155, 913
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 5 cut(s) 131, 490, 711, 844, 873
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 119
MaeIII GTNAC 2 cut(s) 490, 565
MalI GATC 8 cut(s) 179, 189, 311, 770, 783, 924, 954, 969
MbiI CCGCTC 1 cut(s) 104
MboI GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
MboII GAAGA 7 cut(s) 57, 60, 69, 127, 197, 509, 932
MflI RGATCY 3 cut(s) 768, 922, 967
MhlI GDGCHC 1 cut(s) 451
MluCI AATT 1 cut(s) 777
MlyI GAGTC 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 920
MseI TTAA 2 cut(s) 465, 708
MspA1I CMGCKG 1 cut(s) 943
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 4 cut(s) 196, 216, 246, 726
MspR9I CCNGG 7 cut(s) 197, 216, 549, 726, 727, 815, 822
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 3 cut(s) 549, 815, 822
MwoI GCNNNNNNNGC 3 cut(s) 98, 107, 179
NciI CCSGG 4 cut(s) 197, 216, 726, 727
NdeII GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
NlaIII CATG 3 cut(s) 274, 644, 736
NlaIV GGNNCC 3 cut(s) 172, 306, 819
NmeAIII GCCGAG 1 cut(s) 199
NspI RCATGY 1 cut(s) 274
PceI AGGCCT 2 cut(s) 21, 158
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 372, 573
PflFI GACNNNGTC 1 cut(s) 118
PkrI GCNGC 2 cut(s) 103, 194
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PpuMI RGGWCCY 1 cut(s) 817
PshAI GACNNNNGTC 1 cut(s) 248
Psp124BI GAGCTC 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 547, 813, 820
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 3 cut(s) 547, 813, 820
PspN4I GGNNCC 3 cut(s) 172, 306, 819
PspPI GGNCC 4 cut(s) 171, 243, 436, 817
PspPPI RGGWCCY 1 cut(s) 817
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 3 cut(s) 768, 922, 967
PsyI GACNNNGTC 1 cut(s) 118
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
Rsr2I CGGWCCG 1 cut(s) 243
RsrII CGGWCCG 1 cut(s) 243
SacI GAGCTC 1 cut(s) 451
SaqAI TTAA 2 cut(s) 465, 708
SatI GCNGC 2 cut(s) 102, 193
Sau3AI GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
Sau96I GGNCC 4 cut(s) 171, 243, 436, 817
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 7 cut(s) 197, 216, 549, 726, 727, 815, 822
SduI GDGCHC 1 cut(s) 451
SetI ASST 8 cut(s) 122, 152, 351, 451, 592, 682, 819, 826
SfaNI GCATC 5 cut(s) 131, 490, 711, 844, 873
SfcI CTRYAG 2 cut(s) 383, 675
SinI GGWCC 2 cut(s) 243, 817
SmaI CCCGGG 1 cut(s) 727
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SrfI GCCCGGGC 1 cut(s) 727
Sse9I AATT 1 cut(s) 777
SseBI AGGCCT 2 cut(s) 21, 158
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 1 cut(s) 451
StuI AGGCCT 2 cut(s) 21, 158
StyD4I CCNGG 7 cut(s) 195, 214, 547, 724, 725, 813, 820
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 316, 754
TaiI ACGT 1 cut(s) 122
TaqI TCGA 5 cut(s) 14, 204, 236, 327, 881
TasI AATT 1 cut(s) 777
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 2 cut(s) 372, 573
Tru1I TTAA 2 cut(s) 465, 708
Tru9I TTAA 2 cut(s) 465, 708
TseI GCWGC 1 cut(s) 192
TspDTI ATGAA 2 cut(s) 620, 933
TspMI CCCGGG 1 cut(s) 725
Tth111I GACNNNGTC 1 cut(s) 118
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 243, 817
XceI RCATGY 1 cut(s) 274
XmaI CCCGGG 1 cut(s) 725
XspI CTAG 1 cut(s) 300
ZraI GACGTC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.