MD16G1136200.v1.1

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012310
Physical Location & Seq
Forward (+)
10435721 .. 10438655
2935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1136200.v1.1.491

Sequence Viewer

Length: 984 bp
ATGTCGGCGCTATCGAGAGGCCTCATTTCCCGCCTCCGCCTTCTCTCACTCAACCCCTCCTCTTCTTCTTCTCCTCCTACTGCTGCCGCTAATGCTACGACGTCGTTTACGCAGACTCAGTGCCATTTCTTCAGATGGTTCAGCTCTGAGGCCACGGTGGATGCGACGGAGGCCGTCGCGGACAGGAGACCGGTTATCGAAGCGAAGCCAGGGCCGATGAGCCCGGGTTCGAAGAGGACCGGGCTTATAGCCGTCAAGTGCGGGATGACTGCGCTCTGGGACAAATGGGGCGCTAGGGTTCCGATAACTGTGCTCTGGGTCGATGATAATATCGTCTCTCAGGTCAAGACCCCCGAGAAGGAAGGCTTCCCTGCTCTCCAGATTGGTTGCGGACAGAAGAAAGCAAAACATTTGACAAAGCCAGAGGTGGGTCATTTCCGAGCTCAGGGTGTTCCACTTAAGAGGAAATTGCATGAGTTTCCTGTATCAAATGATGCGCTTCTTCCTATTGGTATGGAAATTGGTGTTCGCCATTTTGTACCAGGCCAATATGTTGATGTCACAGGAATCACGCGAGGGAAAGGTTTCCAGGGTGGAATGAAAAGGCACGGTTTTAGTGGTATGCCAGCATCCCATGGCGCATCTCTGTCACACCGAAGTATTGGTTCTACAGGTCAGAGAGATGATGCTGGAAAGGTCTTTAAAGGCAGAAAAATGCCAGGGCGCATGGGTGGGAAACAGAGAACGGTGAAAAATGTATGGGTCTACAAAATTGATCCTGCAAGGAATTTGATGTGGGTGAAAGGCCAAGTCCCGGGTGCTGAAGGAAACTTTGTTTTCATAAGAGATGCTTTCTACAAGAAACCTGATGTTTCATCGCTACCATTTCCAACTTATTTTGCTTCAGAGGATGAGGATCCATCCAAGTTAGAACCACTAGTTGCTGATCTCGGAGAAGTAGATCCGTTCATGGTGGCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

328

Amino Acids

35.6

Weight (kDa)

9.77

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 177 - 260 1.8e-15 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 765
AccII CGCG 2 cut(s) 179, 574
AciI CCGC 6 cut(s) 31, 37, 87, 179, 261, 390
AclWI GGATC 4 cut(s) 770, 911, 924, 956
AcsI RAATTY 1 cut(s) 787
AcuI CTGAAG 3 cut(s) 115, 843, 888
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 1 cut(s) 540
AfiI CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
AflII CTTAAG 1 cut(s) 458
AgeI ACCGGT 1 cut(s) 190
AhlI ACTAGT 1 cut(s) 937
AjnI CCWGG 4 cut(s) 208, 541, 588, 718
AluBI AGCT 2 cut(s) 144, 443
AluI AGCT 2 cut(s) 144, 443
Alw21I GWGCWC 2 cut(s) 315, 445
Alw26I GTCTC 2 cut(s) 181, 340
AlwI GGATC 4 cut(s) 770, 911, 924, 956
Ama87I CYCGRG 3 cut(s) 223, 353, 814
AoxI GGCC 6 cut(s) 19, 150, 171, 212, 544, 805
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 787
AsiGI ACCGGT 1 cut(s) 190
Asp700I GAANNNNTTC 1 cut(s) 584
AspLEI GCGC 6 cut(s) 10, 274, 293, 499, 641, 726
AspS9I GGNCC 2 cut(s) 212, 237
AsuC2I CCSGG 5 cut(s) 224, 225, 241, 815, 816
AsuHPI GGTGA 2 cut(s) 760, 811
AsuII TTCGAA 1 cut(s) 230
AvaI CYCGRG 3 cut(s) 223, 353, 814
AvaII GGWCC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 916
BanII GRGCYC 2 cut(s) 224, 445
Bbv12I GWGCWC 2 cut(s) 315, 445
BbvI GCAGC 1 cut(s) 70
BccI CCATC 2 cut(s) 129, 928
BceAI ACGGC 2 cut(s) 158, 236
BcgI CGANNNNNNTGC 2 cut(s) 292, 326
BciT130I CCWGG 4 cut(s) 210, 543, 590, 720
BcnI CCSGG 5 cut(s) 224, 225, 241, 815, 816
BcoDI GTCTC 2 cut(s) 181, 340
BcuI ACTAGT 1 cut(s) 937
BfaI CTAG 2 cut(s) 294, 938
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 2 cut(s) 11, 294
BfrI CTTAAG 1 cut(s) 458
BisI GCNGC 2 cut(s) 84, 87
BlsI GCNGC 2 cut(s) 85, 88
Bme1390I CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
Bme18I GGWCC 1 cut(s) 237
BmeT110I CYCGRG 3 cut(s) 223, 353, 814
BmgT120I GGNCC 2 cut(s) 212, 237
BmiI GGNNCC 2 cut(s) 300, 918
BmrFI CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
BmsI GCATC 6 cut(s) 151, 484, 638, 650, 676, 838
BpmI CTGGAG 1 cut(s) 362
Bpu10I CCTNAGC 1 cut(s) 444
Bpu14I TTCGAA 1 cut(s) 230
BpuMI CCSGG 5 cut(s) 224, 225, 241, 815, 816
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 7 cut(s) 153, 209, 223, 589, 634, 719, 814
BsaWI WCCGGW 1 cut(s) 190
Bsc4I CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
Bse118I RCCGGY 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 4 cut(s) 210, 543, 590, 720
BseDI CCNNGG 7 cut(s) 153, 209, 223, 589, 634, 719, 814
BseGI GGATG 5 cut(s) 166, 270, 629, 916, 920
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
BseMII CTCAG 4 cut(s) 131, 138, 353, 458
BseRI GAGGAG 2 cut(s) 49, 63
BseXI GCAGC 1 cut(s) 70
Bsh1236I CGCG 2 cut(s) 179, 574
BshFI GGCC 6 cut(s) 21, 152, 173, 214, 546, 807
BshTI ACCGGT 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 315, 445
BsiHKCI CYCGRG 3 cut(s) 223, 353, 814
BsiSI CCGG 4 cut(s) 191, 224, 240, 815
BslFI GGGAC 2 cut(s) 293, 797
BslI CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
BsmAI GTCTC 2 cut(s) 181, 340
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 293, 797
BsnI GGCC 6 cut(s) 21, 152, 173, 214, 546, 807
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 3 cut(s) 223, 353, 814
Bsp119I TTCGAA 1 cut(s) 230
Bsp1286I GDGCHC 3 cut(s) 224, 315, 445
Bsp143I GATC 4 cut(s) 775, 916, 946, 961
Bsp19I CCATGG 1 cut(s) 634
BspACI CCGC 6 cut(s) 31, 37, 87, 179, 261, 390
BspANI GGCC 6 cut(s) 21, 152, 173, 214, 546, 807
BspCNI CTCAG 4 cut(s) 130, 139, 352, 457
BspFNI CGCG 2 cut(s) 179, 574
BspLI GGNNCC 2 cut(s) 300, 918
BspPI GGATC 4 cut(s) 770, 911, 924, 956
BspT104I TTCGAA 1 cut(s) 230
BspTI CTTAAG 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 7 cut(s) 153, 209, 223, 589, 634, 719, 814
BssMI GATC 4 cut(s) 775, 916, 946, 961
BssNI GRCGYC 1 cut(s) 101
BssT1I CCWWGG 1 cut(s) 634
Bst2UI CCWGG 4 cut(s) 210, 543, 590, 720
Bst4CI ACNGT 4 cut(s) 157, 310, 611, 748
Bst6I CTCTTC 2 cut(s) 67, 227
BstACI GRCGYC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 458
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 4 cut(s) 117, 147, 339, 444
BstDSI CCRYGG 2 cut(s) 153, 634
BstF5I GGATG 5 cut(s) 166, 270, 629, 916, 920
BstFNI CGCG 2 cut(s) 179, 574
BstH2I RGCGCY 2 cut(s) 11, 294
BstHHI GCGC 6 cut(s) 10, 274, 293, 499, 641, 726
BstKTI GATC 4 cut(s) 778, 919, 949, 964
BstMAI GTCTC 2 cut(s) 181, 340
BstMBI GATC 4 cut(s) 775, 916, 946, 961
BstMWI GCNNNNNNNGC 2 cut(s) 92, 170
BstNI CCWGG 4 cut(s) 210, 543, 590, 720
BstSCI CCNGG 9 cut(s) 208, 222, 223, 239, 541, 588, 718, 813, 814
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 2 cut(s) 179, 574
BstV1I GCAGC 1 cut(s) 70
BstX2I RGATCY 2 cut(s) 916, 961
BstYI RGATCY 2 cut(s) 916, 961
BsuRI GGCC 6 cut(s) 21, 152, 173, 214, 546, 807
BtgI CCRYGG 2 cut(s) 153, 634
BtgZI GCGATG 1 cut(s) 861
BtsCI GGATG 5 cut(s) 166, 270, 629, 916, 920
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 627
CfoI GCGC 6 cut(s) 10, 274, 293, 499, 641, 726
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 212, 237
Cfr9I CCCGGG 2 cut(s) 223, 814
Csp6I GTAC 1 cut(s) 539
CspAI ACCGGT 1 cut(s) 190
CviAII CATG 4 cut(s) 473, 635, 727, 970
CviQI GTAC 1 cut(s) 539
DdeI CTNAG 4 cut(s) 117, 147, 339, 444
DpnI GATC 4 cut(s) 777, 918, 948, 963
DpnII GATC 4 cut(s) 775, 916, 946, 961
DraI TTTAAA 1 cut(s) 703
Eam1104I CTCTTC 2 cut(s) 67, 227
EarI CTCTTC 2 cut(s) 67, 227
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 634
Eco147I AGGCCT 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 224, 445
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 443
Eco57I CTGAAG 3 cut(s) 115, 843, 888
Eco88I CYCGRG 3 cut(s) 223, 353, 814
EcoICRI GAGCTC 1 cut(s) 443
EcoRII CCWGG 4 cut(s) 208, 541, 588, 718
EcoT14I CCWWGG 1 cut(s) 634
EcoT38I GRGCYC 2 cut(s) 224, 445
ErhI CCWWGG 1 cut(s) 634
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 4 cut(s) 476, 638, 730, 973
FalI AAGNNNNNCTT 4 cut(s) 350, 382, 835, 867
FaqI GGGAC 2 cut(s) 293, 797
FatI CATG 4 cut(s) 472, 634, 726, 969
FauI CCCGC 2 cut(s) 38, 254
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 2 cut(s) 84, 87
FokI GGATG 5 cut(s) 173, 277, 616, 907, 923
FriOI GRGCYC 2 cut(s) 224, 445
Fsp4HI GCNGC 2 cut(s) 84, 87
FspBI CTAG 2 cut(s) 294, 938
GlaI GCGC 6 cut(s) 9, 273, 292, 498, 640, 725
GluI GCNGC 2 cut(s) 84, 87
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 2 cut(s) 11, 294
HaeIII GGCC 6 cut(s) 21, 152, 173, 214, 546, 807
HapII CCGG 4 cut(s) 191, 224, 240, 815
HhaI GCGC 6 cut(s) 10, 274, 293, 499, 641, 726
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 4 cut(s) 476, 638, 730, 973
Hin6I GCGC 6 cut(s) 8, 272, 291, 497, 639, 724
HinP1I GCGC 6 cut(s) 8, 272, 291, 497, 639, 724
HinfI GANTC 2 cut(s) 115, 567
HpaII CCGG 4 cut(s) 191, 224, 240, 815
HphI GGTGA 2 cut(s) 760, 811
Hpy166II GTNNAC 2 cut(s) 108, 766
Hpy188I TCNGA 7 cut(s) 134, 148, 303, 440, 678, 907, 953
Hpy188III TCNNGA 3 cut(s) 15, 346, 379
Hpy8I GTNNAC 2 cut(s) 108, 766
Hpy99I CGWCG 4 cut(s) 103, 106, 169, 179
HpyAV CCTTC 4 cut(s) 50, 352, 356, 818
HpyCH4III ACNGT 4 cut(s) 157, 310, 611, 748
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 2 cut(s) 472, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 170
HpyF3I CTNAG 4 cut(s) 117, 147, 339, 444
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 4 cut(s) 476, 638, 730, 973
HspAI GCGC 6 cut(s) 8, 272, 291, 497, 639, 724
Kzo9I GATC 4 cut(s) 775, 916, 946, 961
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 6 cut(s) 151, 484, 638, 650, 676, 838
MaeI CTAG 2 cut(s) 294, 938
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 2 cut(s) 559, 648
MalI GATC 4 cut(s) 777, 918, 948, 963
MboI GATC 4 cut(s) 775, 916, 946, 961
MboII GAAGA 7 cut(s) 54, 57, 60, 121, 244, 409, 494
MflI RGATCY 2 cut(s) 916, 961
MhlI GDGCHC 3 cut(s) 224, 315, 445
MluCI AATT 4 cut(s) 467, 519, 771, 787
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 584
MseI TTAA 3 cut(s) 459, 702, 982
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 4 cut(s) 191, 224, 240, 815
MspR9I CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
MvaI CCWGG 4 cut(s) 210, 543, 590, 720
MvnI CGCG 2 cut(s) 179, 574
MwoI GCNNNNNNNGC 2 cut(s) 92, 170
NciI CCSGG 5 cut(s) 224, 225, 241, 815, 816
NcoI CCATGG 1 cut(s) 634
NdeII GATC 4 cut(s) 775, 916, 946, 961
NlaIII CATG 4 cut(s) 476, 638, 730, 973
NlaIV GGNNCC 2 cut(s) 300, 918
NmuCI GTSAC 2 cut(s) 559, 648
NspV TTCGAA 1 cut(s) 230
PceI AGGCCT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 161
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 1 cut(s) 567
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 2 cut(s) 85, 88
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
Psp124BI GAGCTC 1 cut(s) 445
Psp6I CCWGG 4 cut(s) 208, 541, 588, 718
PspGI CCWGG 4 cut(s) 208, 541, 588, 718
PspN4I GGNNCC 2 cut(s) 300, 918
PspPI GGNCC 2 cut(s) 212, 237
PsuI RGATCY 2 cut(s) 916, 961
RsaI GTAC 1 cut(s) 540
RsaNI GTAC 1 cut(s) 539
SacI GAGCTC 1 cut(s) 445
SaqAI TTAA 3 cut(s) 459, 702, 982
SatI GCNGC 2 cut(s) 84, 87
Sau3AI GATC 4 cut(s) 775, 916, 946, 961
Sau96I GGNCC 2 cut(s) 212, 237
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
SduI GDGCHC 3 cut(s) 224, 315, 445
SetI ASST 9 cut(s) 104, 146, 345, 429, 445, 586, 676, 699, 868
SfaNI GCATC 6 cut(s) 151, 484, 638, 650, 676, 838
SfcI CTRYAG 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 230
SinI GGWCC 1 cut(s) 237
SmaI CCCGGG 2 cut(s) 225, 816
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SpeI ACTAGT 1 cut(s) 937
Sse9I AATT 4 cut(s) 467, 519, 771, 787
SseBI AGGCCT 1 cut(s) 21
SsiI CCGC 6 cut(s) 31, 37, 87, 179, 261, 390
SspMI CTAG 2 cut(s) 294, 938
SstI GAGCTC 1 cut(s) 445
StuI AGGCCT 1 cut(s) 21
StyD4I CCNGG 9 cut(s) 208, 222, 223, 239, 541, 588, 718, 813, 814
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 4 cut(s) 157, 310, 611, 748
TaiI ACGT 1 cut(s) 104
TaqI TCGA 4 cut(s) 14, 198, 230, 321
TasI AATT 4 cut(s) 467, 519, 771, 787
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 3 cut(s) 459, 702, 982
Tru9I TTAA 3 cut(s) 459, 702, 982
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 2 cut(s) 559, 648
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 2 cut(s) 559, 648
TspDTI ATGAA 4 cut(s) 614, 829, 864, 958
TspGWI ACGGA 2 cut(s) 182, 954
TspMI CCCGGG 2 cut(s) 223, 814
TspRI CASTG 1 cut(s) 125
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 237
XapI RAATTY 1 cut(s) 787
XmaI CCCGGG 2 cut(s) 223, 814
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 2 cut(s) 294, 938
ZraI GACGTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.