Rh4BG280700

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012310
Physical Location & Seq
Reverse (-)
45472961 .. 45475402
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG280700.1

Sequence Viewer

Length: 720 bp
ATGTCGGCGCGCTGGGACAAATGGGGTGCTAGAGTTCCGATCACCGTGCTTTGGGTCGATGATAATATCGTCTCTCAGGTCAAAACCCCAGAGAAAGAAGGAATCTCTGCCCTACAGATTGGGTGCGGGCAGAAAAAAGCAAAACATCTGACAAAGCCTGAAGTGGGCCATTTCAGAGCTCAGGGTGTTCCACTTAAGAGGAAACTGCGGGAGTTTCCTGTAACAGAAGATGCGCTTCTCCCTGTTGGTATGTCAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGACGTTACAGGAATCACGAGAGGGAAAGGTTTTCAGGGTGGGATGAAAAGGTATGGTTTTAGAGGAATGCCAGCAAGTCATGGTGCTTCTAAGTCACACCGGAGTATTGGTTCTACAGGTCAGAGAGATGATGCTGGAAGGGTCTTTAAAGGCAGAAAGATGCCCGGGCGCATGGGTGGAAAACAGAGAACGGTGAAAAATGTATGGATCTACAAAATTGATCCCGCAAGGAACTTGATATGGGTGAGAGGCCAGGTCCCAGGTGCTGAAGGGAACTTTGTGTTTATCAAGGATGCTTTCTACAAGAAACCCGATGTCTCGATGCTACCATTTCCAACTTACTTTGCTCCAGAGGATGAAGATCCAAGCAAGTTAGAACCGCTGGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGCGGCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

239

Amino Acids

26.48

Weight (kDa)

9.87

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 89 - 171 4.3e-17 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 10
AciI CCGC 5 cut(s) 126, 208, 516, 671, 710
AclWI GGATC 4 cut(s) 506, 506, 647, 692
AcuI CTGAAG 2 cut(s) 180, 579
AfiI CCNNNNNNNGG 2 cut(s) 51, 164
AflII CTTAAG 1 cut(s) 194
AjnI CCWGG 3 cut(s) 277, 543, 550
AloI GAACNNNNNNTCC 2 cut(s) 385, 417
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 2 cut(s) 76, 613
AlwI GGATC 4 cut(s) 506, 506, 647, 692
AlwNI CAGNNNCTG 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 3 cut(s) 166, 280, 541
AspLEI GCGC 4 cut(s) 10, 12, 235, 462
AspS9I GGNCC 2 cut(s) 166, 547
AsuC2I CCSGG 2 cut(s) 456, 457
AsuHPI GGTGA 3 cut(s) 34, 496, 547
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 181
BauI CACGAG 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 181
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 3 cut(s) 279, 545, 552
BcnI CCSGG 2 cut(s) 456, 457
BcoDI GTCTC 2 cut(s) 76, 613
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 2 cut(s) 113, 405
BfrI CTTAAG 1 cut(s) 194
BisI GCNGC 1 cut(s) 711
BlsI GCNGC 1 cut(s) 712
Bme1390I CCNGG 5 cut(s) 279, 456, 457, 545, 552
Bme18I GGWCC 1 cut(s) 547
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 2 cut(s) 166, 547
BmiI GGNNCC 1 cut(s) 549
BmrFI CCNGG 5 cut(s) 279, 456, 457, 545, 552
BmsI GCATC 5 cut(s) 220, 412, 441, 574, 603
BpmI CTGGAG 1 cut(s) 624
Bpu10I CCTNAGC 1 cut(s) 180
BpuMI CCSGG 2 cut(s) 456, 457
BsaBI GATNNNNATC 1 cut(s) 651
BsaJI CCNNGG 2 cut(s) 455, 550
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 164
Bse8I GATNNNNATC 1 cut(s) 651
BseBI CCWGG 3 cut(s) 279, 545, 552
BseDI CCNNGG 2 cut(s) 455, 550
BseGI GGATG 3 cut(s) 339, 589, 652
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 2 cut(s) 51, 164
BseMII CTCAG 2 cut(s) 89, 194
BsePI GCGCGC 1 cut(s) 8
BseYI CCCAGC 1 cut(s) 12
Bsh1236I CGCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 168, 282, 543
BsiHKAI GWGCWC 1 cut(s) 181
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 2 cut(s) 391, 456
BslFI GGGAC 2 cut(s) 29, 533
BslI CCNNNNNNNGG 2 cut(s) 51, 164
BsmAI GTCTC 2 cut(s) 76, 613
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 29, 533
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 3 cut(s) 168, 282, 543
BsoBI CYCGRG 1 cut(s) 455
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 6 cut(s) 39, 498, 511, 652, 682, 697
BspACI CCGC 5 cut(s) 126, 208, 516, 671, 710
BspANI GGCC 3 cut(s) 168, 282, 543
BspCNI CTCAG 2 cut(s) 88, 193
BspFNI CGCG 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 549
BspPI GGATC 4 cut(s) 506, 506, 647, 692
BspTI CTTAAG 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 455, 550
BssHII GCGCGC 1 cut(s) 8
BssMI GATC 6 cut(s) 39, 498, 511, 652, 682, 697
BssSI CACGAG 1 cut(s) 307
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 279, 545, 552
Bst4CI ACNGT 2 cut(s) 46, 484
BstAFI CTTAAG 1 cut(s) 194
BstC8I GCNNGC 3 cut(s) 10, 128, 363
BstDEI CTNAG 3 cut(s) 75, 180, 381
BstF5I GGATG 3 cut(s) 339, 589, 652
BstFNI CGCG 1 cut(s) 10
BstHHI GCGC 4 cut(s) 10, 12, 235, 462
BstKTI GATC 6 cut(s) 42, 501, 514, 655, 685, 700
BstMAI GTCTC 2 cut(s) 76, 613
BstMBI GATC 6 cut(s) 39, 498, 511, 652, 682, 697
BstNI CCWGG 3 cut(s) 279, 545, 552
BstSCI CCNGG 5 cut(s) 277, 454, 455, 543, 550
BstSFI CTRYAG 2 cut(s) 113, 405
BstUI CGCG 1 cut(s) 10
BstX2I RGATCY 3 cut(s) 498, 652, 697
BstYI RGATCY 3 cut(s) 498, 652, 697
BsuRI GGCC 3 cut(s) 168, 282, 543
BtsCI GGATG 3 cut(s) 339, 589, 652
Cac8I GCNNGC 3 cut(s) 10, 128, 363
CaiI CAGNNNCTG 1 cut(s) 557
CfoI GCGC 4 cut(s) 10, 12, 235, 462
Cfr13I GGNCC 2 cut(s) 166, 547
Cfr9I CCCGGG 1 cut(s) 455
CviAII CATG 2 cut(s) 371, 463
CviJI RGCY 5 cut(s) 157, 168, 179, 282, 543
CviKI_1 RGCY 5 cut(s) 157, 168, 179, 282, 543
DdeI CTNAG 3 cut(s) 75, 180, 381
DpnI GATC 6 cut(s) 41, 500, 513, 654, 684, 699
DpnII GATC 6 cut(s) 39, 498, 511, 652, 682, 697
DraI TTTAAA 1 cut(s) 439
Ecl136II GAGCTC 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 2 cut(s) 180, 579
Eco88I CYCGRG 1 cut(s) 455
EcoICRI GAGCTC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 547
EcoRII CCWGG 3 cut(s) 277, 543, 550
EcoT38I GRGCYC 1 cut(s) 181
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 2 cut(s) 374, 466
FaiI YATR 8 cut(s) 251, 288, 345, 372, 464, 496, 532, 707
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 2 cut(s) 29, 533
FatI CATG 2 cut(s) 370, 462
FauI CCCGC 3 cut(s) 119, 201, 523
Fnu4HI GCNGC 1 cut(s) 711
FokI GGATG 3 cut(s) 346, 596, 659
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 1 cut(s) 711
FspBI CTAG 1 cut(s) 30
GlaI GCGC 4 cut(s) 9, 11, 234, 461
GluI GCNGC 1 cut(s) 711
GsaI CCCAGC 1 cut(s) 16
GsuI CTGGAG 1 cut(s) 624
HaeIII GGCC 3 cut(s) 168, 282, 543
HapII CCGG 2 cut(s) 391, 456
HhaI GCGC 4 cut(s) 10, 12, 235, 462
Hin1II CATG 2 cut(s) 374, 466
Hin6I GCGC 4 cut(s) 8, 10, 233, 460
HinP1I GCGC 4 cut(s) 8, 10, 233, 460
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 102, 303
HpaII CCGG 2 cut(s) 391, 456
HphI GGTGA 3 cut(s) 34, 496, 547
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 4 cut(s) 39, 150, 176, 414
Hpy188III TCNNGA 3 cut(s) 307, 610, 641
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 3 cut(s) 92, 423, 554
HpyCH4III ACNGT 2 cut(s) 46, 484
HpyCH4IV ACGT 1 cut(s) 294
HpyF3I CTNAG 3 cut(s) 75, 180, 381
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 2 cut(s) 374, 466
HspAI GCGC 4 cut(s) 8, 10, 233, 460
Kzo9I GATC 6 cut(s) 39, 498, 511, 652, 682, 697
LmnI GCTCC 1 cut(s) 643
LweI GCATC 5 cut(s) 220, 412, 441, 574, 603
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 3 cut(s) 220, 295, 384
MalI GATC 6 cut(s) 41, 500, 513, 654, 684, 699
MboI GATC 6 cut(s) 39, 498, 511, 652, 682, 697
MboII GAAGA 2 cut(s) 239, 662
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 3 cut(s) 498, 652, 697
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 2 cut(s) 255, 507
MmeI TCCRAC 1 cut(s) 650
MnlI CCTC 5 cut(s) 192, 305, 347, 533, 637
MseI TTAA 2 cut(s) 195, 438
MspA1I CMGCKG 1 cut(s) 673
MspCI CTTAAG 1 cut(s) 194
MspI CCGG 2 cut(s) 391, 456
MspR9I CCNGG 5 cut(s) 279, 456, 457, 545, 552
MunI CAATTG 1 cut(s) 255
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 3 cut(s) 279, 545, 552
MvnI CGCG 1 cut(s) 10
NciI CCSGG 2 cut(s) 456, 457
NdeII GATC 6 cut(s) 39, 498, 511, 652, 682, 697
NlaIII CATG 2 cut(s) 374, 466
NlaIV GGNNCC 1 cut(s) 549
NmuCI GTSAC 1 cut(s) 384
PauI GCGCGC 1 cut(s) 8
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 2 cut(s) 102, 303
PkrI GCNGC 1 cut(s) 712
PpuMI RGGWCCY 1 cut(s) 547
Psp124BI GAGCTC 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 3 cut(s) 277, 543, 550
PspFI CCCAGC 1 cut(s) 12
PspGI CCWGG 3 cut(s) 277, 543, 550
PspN4I GGNNCC 1 cut(s) 549
PspPI GGNCC 2 cut(s) 166, 547
PspPPI RGGWCCY 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 3 cut(s) 498, 652, 697
PteI GCGCGC 1 cut(s) 8
SacI GAGCTC 1 cut(s) 181
SaqAI TTAA 2 cut(s) 195, 438
SatI GCNGC 1 cut(s) 711
Sau3AI GATC 6 cut(s) 39, 498, 511, 652, 682, 697
Sau96I GGNCC 2 cut(s) 166, 547
ScrFI CCNGG 5 cut(s) 279, 456, 457, 545, 552
SduI GDGCHC 1 cut(s) 181
SetI ASST 8 cut(s) 81, 181, 297, 322, 344, 412, 549, 556
SfaNI GCATC 5 cut(s) 220, 412, 441, 574, 603
SfcI CTRYAG 2 cut(s) 113, 405
SinI GGWCC 1 cut(s) 547
SmaI CCCGGG 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SrfI GCCCGGGC 1 cut(s) 457
Sse9I AATT 2 cut(s) 255, 507
SsiI CCGC 5 cut(s) 126, 208, 516, 671, 710
SspMI CTAG 1 cut(s) 30
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 5 cut(s) 277, 454, 455, 543, 550
TaaI ACNGT 2 cut(s) 46, 484
TaiI ACGT 1 cut(s) 297
TaqI TCGA 2 cut(s) 57, 611
TasI AATT 2 cut(s) 255, 507
TauI GCSGC 1 cut(s) 713
TfiI GAWTC 2 cut(s) 102, 303
Tru1I TTAA 2 cut(s) 195, 438
Tru9I TTAA 2 cut(s) 195, 438
TseFI GTSAC 1 cut(s) 384
Tsp45I GTSAC 1 cut(s) 384
TspDTI ATGAA 2 cut(s) 350, 663
TspMI CCCGGG 1 cut(s) 455
Vha464I CTTAAG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 547
XmaI CCCGGG 1 cut(s) 455
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.