Rw4G023830

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012310
Physical Location & Seq
Reverse (-)
49598444 .. 49601512
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G023830.1

Sequence Viewer

Length: 759 bp
ATGTCCGCCTTATCGAGAGGCCTCATTGCCCGCCTCCGCCTCCTCTCCCTCAACCCGGCCACTTCTTCTTCTTCCTCCTCTTCTACTTGTACCGCTATACCCGCCGCTCACGCTACGACGACGTCGTATTCTCATTTCTTCCGATGCTTCAGCTCCGAGGCCTTGGTGGACGGGGCCGAGATCGCCGAAGATCGCAGCCGGGTTATCGAGGCCAGGCCCGGAGTTATGACCCCCCAATCGAAGCGGACCGGAGTCATCGCCGTCAAGTGTGGCATGTCGGCGCGCTGGGACAAATGGGGTGCTAGGGTTCCGATCACCGTGCTTTGGGTCGATGATAATATCGTCTCTCAGGTCAAAACCCCAGAGAAAGAAGGAATCTCTGCCCTACAGATTGGGTGCGGGCAGAAAAAAGCAAAACATCTGACAAAGCCTGAAGTGGGCCATTTCAGAGCTCAGGGTGTTCCACTTAAGAGGAAACTGCGGGAGTTTCCGGTAACAGAAGATGCGCTTCTCCCTGTTGGTATGTCAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGATGTTACAGGAATCACGAGAGGGAAAGGTTTTCAGGGTGGGATGAAAAGGTATGGTTTTAGAGGAATGCCAGCAAGTCATGGTGCTTCTAAGTCACACCGAAGTATTGGTTCTACAGGTCAGAGAGATGATGCTGGAAGGGTCTTTAAAGGCAGAAAGATGCCCGGGCGCATGGGTGGAAACAGAGAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

252

Amino Acids

27.24

Weight (kDa)

10.6

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 180 - 247 9e-14 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
AccBSI CCGCTC 1 cut(s) 107
AccII CGCG 1 cut(s) 283
AciI CCGC 9 cut(s) 6, 31, 37, 93, 102, 105, 244, 399, 481
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 2 cut(s) 133, 453
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 3 cut(s) 55, 324, 437
AflII CTTAAG 1 cut(s) 467
AjnI CCWGG 2 cut(s) 212, 550
AluBI AGCT 2 cut(s) 153, 452
AluI AGCT 2 cut(s) 153, 452
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 1 cut(s) 349
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 8 cut(s) 19, 57, 159, 174, 210, 215, 439, 553
ApeKI GCWGC 1 cut(s) 195
AspLEI GCGC 4 cut(s) 283, 285, 508, 735
AspS9I GGNCC 4 cut(s) 174, 216, 246, 439
AsuC2I CCSGG 5 cut(s) 56, 200, 219, 729, 730
AsuHPI GGTGA 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 1 cut(s) 246
BanII GRGCYC 1 cut(s) 454
BauI CACGAG 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 1 cut(s) 207
BceAI ACGGC 1 cut(s) 245
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 2 cut(s) 214, 552
BcnI CCSGG 5 cut(s) 56, 200, 219, 729, 730
BcoDI GTCTC 1 cut(s) 349
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 2 cut(s) 386, 678
BfrI CTTAAG 1 cut(s) 467
BisI GCNGC 2 cut(s) 105, 196
BlsI GCNGC 2 cut(s) 106, 197
Bme1390I CCNGG 7 cut(s) 56, 200, 214, 219, 552, 729, 730
Bme18I GGWCC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 728
BmgT120I GGNCC 4 cut(s) 174, 216, 246, 439
BmiI GGNNCC 2 cut(s) 175, 309
BmrFI CCNGG 7 cut(s) 56, 200, 214, 219, 552, 729, 730
BmsI GCATC 4 cut(s) 134, 493, 685, 714
BoxI GACNNNNGTC 1 cut(s) 251
Bpu10I CCTNAGC 1 cut(s) 453
BpuMI CCSGG 5 cut(s) 56, 200, 219, 729, 730
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 156, 162, 728
BsaWI WCCGGW 2 cut(s) 248, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 324, 437
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 2 cut(s) 214, 552
BseDI CCNNGG 3 cut(s) 156, 162, 728
BseGI GGATG 1 cut(s) 612
BseLI CCNNNNNNNGG 3 cut(s) 55, 324, 437
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 2 cut(s) 362, 467
BsePI GCGCGC 1 cut(s) 281
BseRI GAGGAG 2 cut(s) 32, 67
BseXI GCAGC 1 cut(s) 207
BseYI CCCAGC 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 8 cut(s) 21, 59, 161, 176, 212, 217, 441, 555
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 3 cut(s) 55, 324, 437
BsmAI GTCTC 1 cut(s) 349
BsmBI CGTCTC 1 cut(s) 349
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 1 cut(s) 636
BsnI GGCC 8 cut(s) 21, 59, 161, 176, 212, 217, 441, 555
BsoBI CYCGRG 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 3 cut(s) 180, 190, 312
BspACI CCGC 9 cut(s) 6, 31, 37, 93, 102, 105, 244, 399, 481
BspANI GGCC 8 cut(s) 21, 59, 161, 176, 212, 217, 441, 555
BspCNI CTCAG 2 cut(s) 361, 466
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 175, 309
BspTI CTTAAG 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 24
BssECI CCNNGG 3 cut(s) 156, 162, 728
BssHII GCGCGC 1 cut(s) 281
BssMI GATC 3 cut(s) 180, 190, 312
BssNI GRCGYC 1 cut(s) 122
BssSI CACGAG 1 cut(s) 580
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 580
Bst2UI CCWGG 2 cut(s) 214, 552
Bst4CI ACNGT 2 cut(s) 319, 756
Bst6I CTCTTC 1 cut(s) 85
BstACI GRCGYC 1 cut(s) 122
BstAFI CTTAAG 1 cut(s) 467
BstC8I GCNNGC 4 cut(s) 31, 283, 401, 636
BstDEI CTNAG 3 cut(s) 348, 453, 654
BstF5I GGATG 1 cut(s) 612
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 4 cut(s) 283, 285, 508, 735
BstKTI GATC 3 cut(s) 183, 193, 315
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 3 cut(s) 180, 190, 312
BstMWI GCNNNNNNNGC 3 cut(s) 101, 110, 182
BstNI CCWGG 2 cut(s) 214, 552
BstNSI RCATGY 1 cut(s) 277
BstPAI GACNNNNGTC 1 cut(s) 251
BstSCI CCNGG 7 cut(s) 54, 198, 212, 217, 550, 727, 728
BstSFI CTRYAG 2 cut(s) 386, 678
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 207
BsuRI GGCC 8 cut(s) 21, 59, 161, 176, 212, 217, 441, 555
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 1 cut(s) 612
Cac8I GCNNGC 4 cut(s) 31, 283, 401, 636
CfoI GCGC 4 cut(s) 283, 285, 508, 735
Cfr13I GGNCC 4 cut(s) 174, 216, 246, 439
Cfr9I CCCGGG 1 cut(s) 728
CpoI CGGWCCG 1 cut(s) 246
Csp6I GTAC 1 cut(s) 90
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 3 cut(s) 274, 644, 736
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 3 cut(s) 348, 453, 654
DpnI GATC 3 cut(s) 182, 192, 314
DpnII GATC 3 cut(s) 180, 190, 312
DraI TTTAAA 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 452
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 2 cut(s) 21, 161
Eco24I GRGCYC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 246
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 133, 453
Eco88I CYCGRG 1 cut(s) 728
EcoICRI GAGCTC 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 212, 550
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 349
FaeI CATG 3 cut(s) 277, 647, 739
FaiI YATR 8 cut(s) 98, 227, 275, 524, 561, 618, 645, 737
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 302
FatI CATG 3 cut(s) 273, 643, 735
FauI CCCGC 4 cut(s) 38, 109, 392, 474
Fnu4HI GCNGC 2 cut(s) 105, 196
FokI GGATG 1 cut(s) 619
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 2 cut(s) 105, 196
FspBI CTAG 1 cut(s) 303
GlaI GCGC 4 cut(s) 282, 284, 507, 734
GluI GCNGC 2 cut(s) 105, 196
GsaI CCCAGC 1 cut(s) 289
HaeIII GGCC 8 cut(s) 21, 59, 161, 176, 212, 217, 441, 555
HapII CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
HhaI GCGC 4 cut(s) 283, 285, 508, 735
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 3 cut(s) 277, 647, 739
Hin6I GCGC 4 cut(s) 281, 283, 506, 733
HinP1I GCGC 4 cut(s) 281, 283, 506, 733
HinfI GANTC 3 cut(s) 252, 375, 576
HpaII CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 6 cut(s) 143, 157, 312, 423, 449, 687
Hpy188III TCNNGA 2 cut(s) 15, 580
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 3 cut(s) 121, 124, 127
HpyAV CCTTC 2 cut(s) 365, 696
HpyCH4III ACNGT 2 cut(s) 319, 756
HpyCH4IV ACGT 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 110, 182
HpyF3I CTNAG 3 cut(s) 348, 453, 654
HpySE526I ACGT 1 cut(s) 122
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 3 cut(s) 277, 647, 739
HspAI GCGC 4 cut(s) 281, 283, 506, 733
Kzo9I GATC 3 cut(s) 180, 190, 312
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 4 cut(s) 134, 493, 685, 714
MaeI CTAG 1 cut(s) 303
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 3 cut(s) 493, 568, 657
MalI GATC 3 cut(s) 182, 192, 314
MbiI CCGCTC 1 cut(s) 107
MboI GATC 3 cut(s) 180, 190, 312
MboII GAAGA 7 cut(s) 57, 60, 63, 72, 130, 200, 512
MfeI CAATTG 1 cut(s) 528
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 1 cut(s) 528
MlyI GAGTC 1 cut(s) 261
MseI TTAA 2 cut(s) 468, 711
MspCI CTTAAG 1 cut(s) 467
MspI CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
MspR9I CCNGG 7 cut(s) 56, 200, 214, 219, 552, 729, 730
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 636
MvaI CCWGG 2 cut(s) 214, 552
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 3 cut(s) 101, 110, 182
NciI CCSGG 5 cut(s) 56, 200, 219, 729, 730
NdeII GATC 3 cut(s) 180, 190, 312
NlaIII CATG 3 cut(s) 277, 647, 739
NlaIV GGNNCC 2 cut(s) 175, 309
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 657
NspI RCATGY 1 cut(s) 277
PauI GCGCGC 1 cut(s) 281
PceI AGGCCT 2 cut(s) 21, 161
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PctI GAATGC 1 cut(s) 636
PfeI GAWTC 2 cut(s) 375, 576
PflFI GACNNNGTC 1 cut(s) 121
PkrI GCNGC 2 cut(s) 106, 197
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PshAI GACNNNNGTC 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 454
Psp6I CCWGG 2 cut(s) 212, 550
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 2 cut(s) 212, 550
PspN4I GGNNCC 2 cut(s) 175, 309
PspPI GGNCC 4 cut(s) 174, 216, 246, 439
PsyI GACNNNGTC 1 cut(s) 121
PteI GCGCGC 1 cut(s) 281
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 2 cut(s) 468, 711
SatI GCNGC 2 cut(s) 105, 196
Sau3AI GATC 3 cut(s) 180, 190, 312
Sau96I GGNCC 4 cut(s) 174, 216, 246, 439
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 7 cut(s) 56, 200, 214, 219, 552, 729, 730
SduI GDGCHC 1 cut(s) 454
SetI ASST 7 cut(s) 125, 155, 354, 454, 595, 617, 685
SfaNI GCATC 4 cut(s) 134, 493, 685, 714
SfcI CTRYAG 2 cut(s) 386, 678
SinI GGWCC 1 cut(s) 246
SmaI CCCGGG 1 cut(s) 730
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SrfI GCCCGGGC 1 cut(s) 730
Sse9I AATT 1 cut(s) 528
SseBI AGGCCT 2 cut(s) 21, 161
SsiI CCGC 9 cut(s) 6, 31, 37, 93, 102, 105, 244, 399, 481
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 1 cut(s) 454
StuI AGGCCT 2 cut(s) 21, 161
StyD4I CCNGG 7 cut(s) 54, 198, 212, 217, 550, 727, 728
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 319, 756
TaiI ACGT 1 cut(s) 125
TaqI TCGA 4 cut(s) 14, 207, 239, 330
TasI AATT 1 cut(s) 528
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 2 cut(s) 375, 576
Tru1I TTAA 2 cut(s) 468, 711
Tru9I TTAA 2 cut(s) 468, 711
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 1 cut(s) 623
TspMI CCCGGG 1 cut(s) 728
Tth111I GACNNNGTC 1 cut(s) 121
Vha464I CTTAAG 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 246
XceI RCATGY 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 728
XspI CTAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.