Rh4AG274900

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012310
Physical Location & Seq
Reverse (-)
59101051 .. 59104125
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG274900.1

Sequence Viewer

Length: 996 bp
ATGTCCGCCTTATCGAGAGGCCTCATTTCCCGCCTCCGCCTCCGCCTCCTCTCCCTCAACCCCGCCACTTCTTCTTCCTCCTCTTCTACTTGTACCGCTATACCCGCCGCTCACGCTACGACGACGTCGTATTCTCATTTCTTCCGATGCTTCAGCTCCGAGGCCTTGGTGGACGGGGCCGAGATCGCCGAAGATCGCAGCCGGATTATCGAGGCCAGGCCCGGAGTTATGACCCCCCAATCGAAGCGGACCGGAGTCATCGCCGTCAAGTGTGGCATGTCGGCGCGCTGGGACAAATGGGGTGCTAGAGTTCCGATCACCGTGCTTTGGGTCGATGATAATATCGTCTCTCAGGTCAAAACCCCAGAGAAAGAAGGAATCTCTGCCCTACAGATTGGGTGCGGGCAGAAAAAAGCAAAACATCTGACAAAGCCTGAAGTGGGCCATTTCAGAGCTCAGGGTGTTCCACTTAAGAGGAAACTGCGGGAGTTTCCTGTAACAGAAGATGCGCTTCTCCCTGTTGGTATGTCAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGACGTTACAGGAATCACGAGAGGGAAAGGTTTTCAGGGTGGGATGAAAAGGTATGGTTTTAGAGGAATGCCAGCAAGTCATGGTGCTTCTAAGTCACACCGGAGTATTGGTTCTACAGGTCAGAGAGATGATGCTGGAAGGGTCTTTAAAGGCAGAAAGATGCCCGGGCGCATGGGTGGAAAACAGAGAACGGTGAAAAATGTATGGATCTACAAAATTGATCCCGCAAGGAACTTGATATGGGTGAGAGGCCAGGTCCCAGGTGCTGAAGGGAACTTTGTGTTTATCAAGGATGCTTTCTACAAGAAACCCGATGTCTCGATGCTACCATTTCCAACTTACTTTGCTCCAGAGGATGAAGATCCAAGCAAGTTAGAACCGCTGGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGCGGCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

331

Amino Acids

36.22

Weight (kDa)

9.92

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 181 - 263 9.5e-17 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 128
AccBSI CCGCTC 1 cut(s) 110
AccII CGCG 1 cut(s) 286
AclWI GGATC 4 cut(s) 782, 782, 923, 968
AcuI CTGAAG 3 cut(s) 136, 456, 855
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 2 cut(s) 327, 440
AflII CTTAAG 1 cut(s) 470
AjnI CCWGG 4 cut(s) 215, 553, 819, 826
AloI GAACNNNNNNTCC 2 cut(s) 661, 693
AluBI AGCT 2 cut(s) 156, 455
AluI AGCT 2 cut(s) 156, 455
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 352, 889
AlwI GGATC 4 cut(s) 782, 782, 923, 968
AlwNI CAGNNNCTG 1 cut(s) 833
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 8 cut(s) 19, 162, 177, 213, 218, 442, 556, 817
ApeKI GCWGC 1 cut(s) 198
AspLEI GCGC 4 cut(s) 286, 288, 511, 738
AspS9I GGNCC 5 cut(s) 177, 219, 249, 442, 823
AsuC2I CCSGG 3 cut(s) 222, 732, 733
AsuHPI GGTGA 3 cut(s) 310, 772, 823
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 2 cut(s) 249, 823
BanII GRGCYC 1 cut(s) 457
BauI CACGAG 1 cut(s) 583
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 1 cut(s) 210
BceAI ACGGC 1 cut(s) 248
BcgI CGANNNNNNTGC 2 cut(s) 304, 338
BciT130I CCWGG 4 cut(s) 217, 555, 821, 828
BcnI CCSGG 3 cut(s) 222, 732, 733
BcoDI GTCTC 2 cut(s) 352, 889
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 2 cut(s) 389, 681
BfrI CTTAAG 1 cut(s) 470
BisI GCNGC 3 cut(s) 108, 199, 987
BlsI GCNGC 3 cut(s) 109, 200, 988
Bme1390I CCNGG 7 cut(s) 217, 222, 555, 732, 733, 821, 828
Bme18I GGWCC 2 cut(s) 249, 823
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 5 cut(s) 177, 219, 249, 442, 823
BmiI GGNNCC 2 cut(s) 178, 825
BmrFI CCNGG 7 cut(s) 217, 222, 555, 732, 733, 821, 828
BmsI GCATC 6 cut(s) 137, 496, 688, 717, 850, 879
BoxI GACNNNNGTC 1 cut(s) 254
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 1 cut(s) 456
BpuMI CCSGG 3 cut(s) 222, 732, 733
BsaBI GATNNNNATC 1 cut(s) 927
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 4 cut(s) 159, 165, 731, 826
BsaWI WCCGGW 2 cut(s) 251, 666
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 440
Bse8I GATNNNNATC 1 cut(s) 927
BseBI CCWGG 4 cut(s) 217, 555, 821, 828
BseDI CCNNGG 4 cut(s) 159, 165, 731, 826
BseGI GGATG 3 cut(s) 615, 865, 928
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 2 cut(s) 327, 440
BseMII CTCAG 2 cut(s) 365, 470
BsePI GCGCGC 1 cut(s) 284
BseRI GAGGAG 2 cut(s) 38, 70
BseXI GCAGC 1 cut(s) 210
BseYI CCCAGC 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 286
BshFI GGCC 8 cut(s) 21, 164, 179, 215, 220, 444, 558, 819
BsiHKAI GWGCWC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 5 cut(s) 202, 222, 252, 667, 732
BslFI GGGAC 2 cut(s) 305, 809
BslI CCNNNNNNNGG 2 cut(s) 327, 440
BsmAI GTCTC 2 cut(s) 352, 889
BsmBI CGTCTC 1 cut(s) 352
BsmFI GGGAC 2 cut(s) 305, 809
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 8 cut(s) 21, 164, 179, 215, 220, 444, 558, 819
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 1 cut(s) 457
Bsp143I GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
BspANI GGCC 8 cut(s) 21, 164, 179, 215, 220, 444, 558, 819
BspCNI CTCAG 2 cut(s) 364, 469
BspFNI CGCG 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 178, 825
BspPI GGATC 4 cut(s) 782, 782, 923, 968
BspTI CTTAAG 1 cut(s) 470
BsrBI CCGCTC 1 cut(s) 110
BssECI CCNNGG 4 cut(s) 159, 165, 731, 826
BssHII GCGCGC 1 cut(s) 284
BssMI GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 583
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 583
Bst2UI CCWGG 4 cut(s) 217, 555, 821, 828
Bst4CI ACNGT 2 cut(s) 322, 760
Bst6I CTCTTC 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 125
BstAFI CTTAAG 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 286, 404, 639
BstDEI CTNAG 3 cut(s) 351, 456, 657
BstF5I GGATG 3 cut(s) 615, 865, 928
BstFNI CGCG 1 cut(s) 286
BstHHI GCGC 4 cut(s) 286, 288, 511, 738
BstKTI GATC 8 cut(s) 186, 196, 318, 777, 790, 931, 961, 976
BstMAI GTCTC 2 cut(s) 352, 889
BstMBI GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
BstMWI GCNNNNNNNGC 3 cut(s) 104, 113, 185
BstNI CCWGG 4 cut(s) 217, 555, 821, 828
BstNSI RCATGY 1 cut(s) 280
BstPAI GACNNNNGTC 1 cut(s) 254
BstSCI CCNGG 7 cut(s) 215, 220, 553, 730, 731, 819, 826
BstSFI CTRYAG 2 cut(s) 389, 681
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 210
BstX2I RGATCY 3 cut(s) 774, 928, 973
BstYI RGATCY 3 cut(s) 774, 928, 973
BsuRI GGCC 8 cut(s) 21, 164, 179, 215, 220, 444, 558, 819
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 615, 865, 928
Cac8I GCNNGC 3 cut(s) 286, 404, 639
CaiI CAGNNNCTG 1 cut(s) 833
CfoI GCGC 4 cut(s) 286, 288, 511, 738
Cfr13I GGNCC 5 cut(s) 177, 219, 249, 442, 823
Cfr9I CCCGGG 1 cut(s) 731
CpoI CGGWCCG 1 cut(s) 249
Csp6I GTAC 1 cut(s) 93
CspI CGGWCCG 1 cut(s) 249
CviAII CATG 3 cut(s) 277, 647, 739
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 3 cut(s) 351, 456, 657
DpnI GATC 8 cut(s) 185, 195, 317, 776, 789, 930, 960, 975
DpnII GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
DraI TTTAAA 1 cut(s) 715
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
EciI GGCGGA 2 cut(s) 26, 32
Ecl136II GAGCTC 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 165
Eco147I AGGCCT 2 cut(s) 21, 164
Eco24I GRGCYC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 249, 823
Eco53kI GAGCTC 1 cut(s) 455
Eco57I CTGAAG 3 cut(s) 136, 456, 855
Eco88I CYCGRG 1 cut(s) 731
EcoICRI GAGCTC 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 823
EcoRII CCWGG 4 cut(s) 215, 553, 819, 826
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 3 cut(s) 280, 650, 742
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 2 cut(s) 305, 809
FatI CATG 3 cut(s) 276, 646, 738
FauI CCCGC 6 cut(s) 38, 70, 112, 395, 477, 799
Fnu4HI GCNGC 3 cut(s) 108, 199, 987
FokI GGATG 3 cut(s) 622, 872, 935
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 3 cut(s) 108, 199, 987
FspBI CTAG 1 cut(s) 306
GlaI GCGC 4 cut(s) 285, 287, 510, 737
GluI GCNGC 3 cut(s) 108, 199, 987
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 8 cut(s) 21, 164, 179, 215, 220, 444, 558, 819
HapII CCGG 5 cut(s) 202, 222, 252, 667, 732
HhaI GCGC 4 cut(s) 286, 288, 511, 738
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 3 cut(s) 280, 650, 742
Hin6I GCGC 4 cut(s) 284, 286, 509, 736
HinP1I GCGC 4 cut(s) 284, 286, 509, 736
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 3 cut(s) 255, 378, 579
HpaII CCGG 5 cut(s) 202, 222, 252, 667, 732
HphI GGTGA 3 cut(s) 310, 772, 823
Hpy166II GTNNAC 2 cut(s) 172, 568
Hpy188I TCNGA 6 cut(s) 146, 160, 315, 426, 452, 690
Hpy188III TCNNGA 4 cut(s) 15, 583, 886, 917
Hpy8I GTNNAC 2 cut(s) 172, 568
Hpy99I CGWCG 3 cut(s) 124, 127, 130
HpyAV CCTTC 3 cut(s) 368, 699, 830
HpyCH4III ACNGT 2 cut(s) 322, 760
HpyCH4IV ACGT 2 cut(s) 125, 570
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 113, 185
HpyF3I CTNAG 3 cut(s) 351, 456, 657
HpySE526I ACGT 2 cut(s) 125, 570
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 3 cut(s) 280, 650, 742
HspAI GCGC 4 cut(s) 284, 286, 509, 736
Kzo9I GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
LmnI GCTCC 2 cut(s) 161, 919
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 6 cut(s) 137, 496, 688, 717, 850, 879
MaeI CTAG 1 cut(s) 306
MaeII ACGT 2 cut(s) 125, 570
MaeIII GTNAC 3 cut(s) 496, 571, 660
MalI GATC 8 cut(s) 185, 195, 317, 776, 789, 930, 960, 975
MbiI CCGCTC 1 cut(s) 110
MboI GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
MboII GAAGA 7 cut(s) 63, 66, 75, 133, 203, 515, 938
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 3 cut(s) 774, 928, 973
MhlI GDGCHC 1 cut(s) 457
MluCI AATT 2 cut(s) 531, 783
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 1 cut(s) 926
MseI TTAA 2 cut(s) 471, 714
MspA1I CMGCKG 1 cut(s) 949
MspCI CTTAAG 1 cut(s) 470
MspI CCGG 5 cut(s) 202, 222, 252, 667, 732
MspR9I CCNGG 7 cut(s) 217, 222, 555, 732, 733, 821, 828
MunI CAATTG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 4 cut(s) 217, 555, 821, 828
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 3 cut(s) 104, 113, 185
NciI CCSGG 3 cut(s) 222, 732, 733
NdeII GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
NlaIII CATG 3 cut(s) 280, 650, 742
NlaIV GGNNCC 2 cut(s) 178, 825
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 660
NspI RCATGY 1 cut(s) 280
PauI GCGCGC 1 cut(s) 284
PceI AGGCCT 2 cut(s) 21, 164
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 2 cut(s) 378, 579
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 3 cut(s) 109, 200, 988
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PpuMI RGGWCCY 1 cut(s) 823
PshAI GACNNNNGTC 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 457
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 4 cut(s) 215, 553, 819, 826
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 4 cut(s) 215, 553, 819, 826
PspN4I GGNNCC 2 cut(s) 178, 825
PspPI GGNCC 5 cut(s) 177, 219, 249, 442, 823
PspPPI RGGWCCY 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 3 cut(s) 774, 928, 973
PsyI GACNNNGTC 1 cut(s) 124
PteI GCGCGC 1 cut(s) 284
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
Rsr2I CGGWCCG 1 cut(s) 249
RsrII CGGWCCG 1 cut(s) 249
SacI GAGCTC 1 cut(s) 457
SaqAI TTAA 2 cut(s) 471, 714
SatI GCNGC 3 cut(s) 108, 199, 987
Sau3AI GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
Sau96I GGNCC 5 cut(s) 177, 219, 249, 442, 823
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 7 cut(s) 217, 222, 555, 732, 733, 821, 828
SduI GDGCHC 1 cut(s) 457
SfaNI GCATC 6 cut(s) 137, 496, 688, 717, 850, 879
SfcI CTRYAG 2 cut(s) 389, 681
SinI GGWCC 2 cut(s) 249, 823
SmaI CCCGGG 1 cut(s) 733
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
SrfI GCCCGGGC 1 cut(s) 733
Sse9I AATT 2 cut(s) 531, 783
SseBI AGGCCT 2 cut(s) 21, 164
SspMI CTAG 1 cut(s) 306
SstI GAGCTC 1 cut(s) 457
StuI AGGCCT 2 cut(s) 21, 164
StyD4I CCNGG 7 cut(s) 215, 220, 553, 730, 731, 819, 826
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 322, 760
TaiI ACGT 2 cut(s) 128, 573
TaqI TCGA 5 cut(s) 14, 210, 242, 333, 887
TasI AATT 2 cut(s) 531, 783
TauI GCSGC 2 cut(s) 110, 989
TfiI GAWTC 2 cut(s) 378, 579
Tru1I TTAA 2 cut(s) 471, 714
Tru9I TTAA 2 cut(s) 471, 714
TseFI GTSAC 1 cut(s) 660
TseI GCWGC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 660
TspDTI ATGAA 2 cut(s) 626, 939
TspMI CCCGGG 1 cut(s) 731
Tth111I GACNNNGTC 1 cut(s) 124
Vha464I CTTAAG 1 cut(s) 470
VpaK11BI GGWCC 2 cut(s) 249, 823
XceI RCATGY 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 731
XspI CTAG 1 cut(s) 306
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.